Journal: Antioxidants

Article Title: Dietary Curcumin Attenuates Hepatic Cellular Senescence by Suppressing the MAPK/NF-κB Signaling Pathway in Aged Mice

doi: 10.3390/antiox12061165

Figure Lengend Snippet: Curcumin ameliorates hepatic cellular senescence by regulating the p38-MAPK signaling pathway in HFHSD-fed aged mice. Immunoblots and protein expression levels in the liver tissue lysates after 15 weeks of dietary interventions: ( A ) p-ERK1/2, ERK1/2, and p-ERK/ERK ratio; ( B ) p-JNK, JNK, and p-JNK/JNK ratio; ( C ) p-p38, p38, and p-p38/p38 ratio; and ( D ) p-MK2, MK2, and p-MK2/MK2 ratio. The results are expressed as mean ± SEM (* p < 0.05).

Article Snippet: The membranes were blocked in a blocking reagent (LI-COR, Lincoln, NE, USA) at room temperature for 1 h and incubated with the primary antibodies at 4 °C overnight as follows: phosphorylated extracellular signal-regulated kinase (p-ERK) 1/2, ERK1/2, phosphorylated c-Jun N-terminal kinase (p-JNK) 1/2, JNK1/2, p-p38, p38, phosphorylated MAP kinase-activated protein kinase 2 (p-MK2), MK2, p-p65, p65, p53, cyclophilin B, histone deacetylase 1 (HDAC1), β-tubulin, and β-actin from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Western Blot, Expressing

Journal: International Journal of Nanomedicine

Article Title: Effects triggered by platinum nanoparticles on primary keratinocytes

doi: 10.2147/IJN.S49612

Figure Lengend Snippet: ( A – D ) Activation of ERK, Akt, p38, and JNK by Western blot analysis. Normal human epidermal keratinocyte cells were cultured in 60 mm tissue-culture dishes at a density of 9.5 × 103 cells/cm 2 . The cells were exposed to 12.5 and 25 μg/mL PtNPs for 0.5, 4, and 24 hours. Untreated cells, harvested together at the 0.5-hour time point, served as a control. At the time of NP addition, the whole medium was exchanged in both treated and untreated cells. Tubulin was used as an internal control to monitor for equal loading. The data are representative of three independent experiments. Abbreviations: PtNPs, platinum nanoparticles; p-ERK, phosphorylated extracellular signal-regulated kinase; p-Akt, phosphorylated protein kinase B; p-JNK, phosphorylated c-Jun N-terminal kinase; Akt, protein kinase B.

Article Snippet: The following antibodies and dilutions were used: Akt (1:1,000; Cell Signaling Technology, Danvers, MA, USA), Phospho-Akt (1:2,000; Cell Signaling Technology), stress-activated protein kinase (SAPK)/c-Jun N-terminal kinase (JNK) (1:500; Cell Signaling Technology), Phospho-SAPK/JNK (1:500; Cell Signaling Technology), p38 MAPK (1:1,000; Cell Signaling Technology), Phospho-p38 MAPK (1:500; Cell Signaling Technology), phosphorylated extracellular signal-regulated kinase (p-ERK)-1/2 MAPK (1:2,000; Cell Signaling Technology), Phospho-ERK1/2 MAPK (1:1,000; Cell Signaling Technology).

Techniques: Activation Assay, Western Blot, Cell Culture