phosphorylated akt  (Cell Signaling Technology Inc)

 
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    Name:
    Akt Antibody
    Description:
    Akt also referred to as PKB or Rac plays a critical role in controlling survival and apoptosis 1 3 This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin sensitive pathway involving PI3 kinase 2 3 Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 4 and by phosphorylation within the carboxy terminus at Ser473 The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin mTOR in a rapamycin insensitive complex with rictor and Sin1 5 6 Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets including Bad 7 forkhead transcription factors 8 c Raf 9 and caspase 9 PTEN phosphatase is a major negative regulator of the PI3 kinase Akt signaling pathway 10 LY294002 is a specific PI3 kinase inhibitor 11 Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK 3α and β 12 13 Akt may also play a role in insulin stimulation of glucose transport 12 In addition to its role in survival and glycogen synthesis Akt is involved in cell cycle regulation by preventing GSK 3β mediated phosphorylation and degradation of cyclin D1 14 and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip1 15 and p21 Waf1 Cip1 16 Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin sensitive complex containing raptor 17 More importantly Akt phosphorylates and inactivates tuberin TSC2 an inhibitor of mTOR within the mTOR raptor complex 18 19
    Catalog Number:
    9272
    Price:
    None
    Category:
    Primary Antibodies
    Source:
    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of mouse Akt. Antibodies are purified by protein A and peptide affinity chromatography.
    Reactivity:
    Human Mouse Rat Hamster Monkey Chicken D melanogaster Bovine Dog Pig Guinea Pig
    Applications:
    Western Blot, Immunoprecipitation, Immunofluorescence, Flow Cytometry
    Buy from Supplier


    Structured Review

    Cell Signaling Technology Inc phosphorylated akt
    Time-dependent effects of pioglitazone on podocyte survival and cytoskeletal proteins and gene expression. ( A ) Decreased phosphorylated <t>Akt</t> was significantly restored when Pio was given with <t>PAN</t> or after (Pio0, PostPio), but not by pretreatment with Pio (prePio); *P
    Akt also referred to as PKB or Rac plays a critical role in controlling survival and apoptosis 1 3 This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin sensitive pathway involving PI3 kinase 2 3 Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 4 and by phosphorylation within the carboxy terminus at Ser473 The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin mTOR in a rapamycin insensitive complex with rictor and Sin1 5 6 Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets including Bad 7 forkhead transcription factors 8 c Raf 9 and caspase 9 PTEN phosphatase is a major negative regulator of the PI3 kinase Akt signaling pathway 10 LY294002 is a specific PI3 kinase inhibitor 11 Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK 3α and β 12 13 Akt may also play a role in insulin stimulation of glucose transport 12 In addition to its role in survival and glycogen synthesis Akt is involved in cell cycle regulation by preventing GSK 3β mediated phosphorylation and degradation of cyclin D1 14 and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip1 15 and p21 Waf1 Cip1 16 Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin sensitive complex containing raptor 17 More importantly Akt phosphorylates and inactivates tuberin TSC2 an inhibitor of mTOR within the mTOR raptor complex 18 19
    https://www.bioz.com/result/phosphorylated akt/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated akt - by Bioz Stars, 2021-03
    86/100 stars

    Images

    1) Product Images from "Protective effects of PPAR? agonist in acute nephrotic syndrome"

    Article Title: Protective effects of PPAR? agonist in acute nephrotic syndrome

    Journal: Nephrology Dialysis Transplantation

    doi: 10.1093/ndt/gfr240

    Time-dependent effects of pioglitazone on podocyte survival and cytoskeletal proteins and gene expression. ( A ) Decreased phosphorylated Akt was significantly restored when Pio was given with PAN or after (Pio0, PostPio), but not by pretreatment with Pio (prePio); *P
    Figure Legend Snippet: Time-dependent effects of pioglitazone on podocyte survival and cytoskeletal proteins and gene expression. ( A ) Decreased phosphorylated Akt was significantly restored when Pio was given with PAN or after (Pio0, PostPio), but not by pretreatment with Pio (prePio); *P

    Techniques Used: Expressing

    2) Product Images from "Over-expression of cathepsin B in hepatocellular carcinomas predicts poor prognosis of HCC patients"

    Article Title: Over-expression of cathepsin B in hepatocellular carcinomas predicts poor prognosis of HCC patients

    Journal: Molecular Cancer

    doi: 10.1186/s12943-016-0503-9

    CTSB regulates MMP-9 through PI3K/Akt signal pathway. a Stable expression of CTSB in MHCC-97 L increased the protein level of p-Akt and MMP-9. b Down-regulation of CTSB in MHCC-97H decreased the protein level of p-Akt and MMP-9. c MMP-9 protein of MHCC-97H/CTSB-shRNA cells stably transfected with pcDNA-MMP-9 increased most significant compared to MHCC-97H/CTSB-shRNA cells. d CTSB knockdown cells stably transfected with MMP-9 showed increased invasive ability. e Treatment of MHCC-97 L/CTSB cells with LY294002, a PI3K inhibitor, led to down-regulation of MMP-9 at protein levels. f MHCC-97 L/CTSB cells treated with LY294002 show decreased invasive ability. (** P
    Figure Legend Snippet: CTSB regulates MMP-9 through PI3K/Akt signal pathway. a Stable expression of CTSB in MHCC-97 L increased the protein level of p-Akt and MMP-9. b Down-regulation of CTSB in MHCC-97H decreased the protein level of p-Akt and MMP-9. c MMP-9 protein of MHCC-97H/CTSB-shRNA cells stably transfected with pcDNA-MMP-9 increased most significant compared to MHCC-97H/CTSB-shRNA cells. d CTSB knockdown cells stably transfected with MMP-9 showed increased invasive ability. e Treatment of MHCC-97 L/CTSB cells with LY294002, a PI3K inhibitor, led to down-regulation of MMP-9 at protein levels. f MHCC-97 L/CTSB cells treated with LY294002 show decreased invasive ability. (** P

    Techniques Used: Expressing, shRNA, Stable Transfection, Transfection

    3) Product Images from "Oncogenic role of ABHD5 in endometrial cancer"

    Article Title: Oncogenic role of ABHD5 in endometrial cancer

    Journal: Cancer Management and Research

    doi: 10.2147/CMAR.S188648

    The activation of AKT is necessary for the biological functions of ABHD5 in endometrial cancer cells. Notes: Ishikawa cells were transfected with pcDNA3.1–ABHD5 or pcDNA3.1 and treated with 10 µM MK-2206 (MK; Merck Millipore, Billerica, MA, USA) or vehicle (DMSO). ( A ) Cell proliferation was detected by CCK-8 assay (n=3 biological replicates). ( B ) Cell invasion was detected by Transwell assay (n=3 biological replicates), magnification 200×. ( C ) 2-NBDG uptake was measured (n=3 biological replicates). Fluorescent intensity was normalized to protein content and then divided by the value of the control (the first column). ( D ) Lactate production (n=3 biological replicates) was measured and divided by the value of the control (the first column). ** P
    Figure Legend Snippet: The activation of AKT is necessary for the biological functions of ABHD5 in endometrial cancer cells. Notes: Ishikawa cells were transfected with pcDNA3.1–ABHD5 or pcDNA3.1 and treated with 10 µM MK-2206 (MK; Merck Millipore, Billerica, MA, USA) or vehicle (DMSO). ( A ) Cell proliferation was detected by CCK-8 assay (n=3 biological replicates). ( B ) Cell invasion was detected by Transwell assay (n=3 biological replicates), magnification 200×. ( C ) 2-NBDG uptake was measured (n=3 biological replicates). Fluorescent intensity was normalized to protein content and then divided by the value of the control (the first column). ( D ) Lactate production (n=3 biological replicates) was measured and divided by the value of the control (the first column). ** P

    Techniques Used: Activation Assay, Transfection, CCK-8 Assay, Transwell Assay

    4) Product Images from "Eef1a2 Promotes Cell Growth, Inhibits Apoptosis and Activates JAK/STAT and AKT Signaling in Mouse Plasmacytomas"

    Article Title: Eef1a2 Promotes Cell Growth, Inhibits Apoptosis and Activates JAK/STAT and AKT Signaling in Mouse Plasmacytomas

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0010755

    Functional changes after Eef1a2 knockdown. (A) Functional classification of differentially expressed genes by Eef1a2 knockdown cells. (B) Downregulation of Eef1a2 impaired IL-6-induced AKT and STAT3 phosphorylation. Eef1a2 shRNA and control cells were treated with 100 ng/ml recombinant IL-6 and protein samples were prepared 15 and 30 min later. Western blot analyses were performed using the indicated antibodies. ( C ) qPCR analyses of gene expression levels in transiently transfected shRNAs in PCT-AP cell line with four plasmids expressing specifically targeting Eef1a2 (shRNA-1,2,3,4) and a control plasmid (shRNA-C). Error bar = ± S.E.
    Figure Legend Snippet: Functional changes after Eef1a2 knockdown. (A) Functional classification of differentially expressed genes by Eef1a2 knockdown cells. (B) Downregulation of Eef1a2 impaired IL-6-induced AKT and STAT3 phosphorylation. Eef1a2 shRNA and control cells were treated with 100 ng/ml recombinant IL-6 and protein samples were prepared 15 and 30 min later. Western blot analyses were performed using the indicated antibodies. ( C ) qPCR analyses of gene expression levels in transiently transfected shRNAs in PCT-AP cell line with four plasmids expressing specifically targeting Eef1a2 (shRNA-1,2,3,4) and a control plasmid (shRNA-C). Error bar = ± S.E.

    Techniques Used: Functional Assay, shRNA, Recombinant, Western Blot, Real-time Polymerase Chain Reaction, Expressing, Transfection, Plasmid Preparation

    5) Product Images from "Protective role of 5-azacytidine on myocardial infarction is associated with modulation of macrophage phenotype and inhibition of fibrosis"

    Article Title: Protective role of 5-azacytidine on myocardial infarction is associated with modulation of macrophage phenotype and inhibition of fibrosis

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.12248

    Effect of 5AZ on angiotensin II (AngII)-stimulated cardiac fibroblasts. (A) The cell number of cardiac fibroblasts was significantly increased after treatment with AngII for 5 days, and 5AZ blocked AngII-induced proliferation. (B) Increased mRNA of connective tissue growth factor (CTGF) and collagen type I (ColI), fibrosis-mediators, was reduced after 5AZ treatment. (C) AngII-induced expression of phosphorylated extracellular signal-regulated kinase, phosphorylated Akt and bcl-2 was reduced after 5AZ treatment. In bar graphs; (1) control; (2) AngII 0.1 μM for 1 day; (3) AngII 0.1 μM + 5AZ for 1 day; (4) AngII 1 μM for 1 day; (5) AngII 1 μM + 5AZ for 1 day; (6) AngII 0.1 μM for 5 days; (7) AngII 0.1 μM + 5AZ for 5 days; (8) AngII 1 μM for 5 days; (9) AngII 1 μM + 5AZ for 5 days.
    Figure Legend Snippet: Effect of 5AZ on angiotensin II (AngII)-stimulated cardiac fibroblasts. (A) The cell number of cardiac fibroblasts was significantly increased after treatment with AngII for 5 days, and 5AZ blocked AngII-induced proliferation. (B) Increased mRNA of connective tissue growth factor (CTGF) and collagen type I (ColI), fibrosis-mediators, was reduced after 5AZ treatment. (C) AngII-induced expression of phosphorylated extracellular signal-regulated kinase, phosphorylated Akt and bcl-2 was reduced after 5AZ treatment. In bar graphs; (1) control; (2) AngII 0.1 μM for 1 day; (3) AngII 0.1 μM + 5AZ for 1 day; (4) AngII 1 μM for 1 day; (5) AngII 1 μM + 5AZ for 1 day; (6) AngII 0.1 μM for 5 days; (7) AngII 0.1 μM + 5AZ for 5 days; (8) AngII 1 μM for 5 days; (9) AngII 1 μM + 5AZ for 5 days.

    Techniques Used: Expressing

    6) Product Images from "Erythropoietin administration partially prevents adipose tissue loss in experimental cancer cachexia models"

    Article Title: Erythropoietin administration partially prevents adipose tissue loss in experimental cancer cachexia models

    Journal: Journal of Lipid Research

    doi: 10.1194/jlr.M038406

    Representative patterns (lower) and densitometric analysis (upper) of (A) EPOR and (B) phosphorylated (Ser473) and total Akt expression measured in WAT protein extracts. C, control mice; EPO, erythropoietin administered; LLC, Lewis lung carcinoma-bearing
    Figure Legend Snippet: Representative patterns (lower) and densitometric analysis (upper) of (A) EPOR and (B) phosphorylated (Ser473) and total Akt expression measured in WAT protein extracts. C, control mice; EPO, erythropoietin administered; LLC, Lewis lung carcinoma-bearing

    Techniques Used: Expressing, Mouse Assay

    Related Articles

    Lysis:

    Article Title: Curcumin inhibits Akt/mTOR signaling through protein phosphatase-dependent mechanism *
    Article Snippet: [6-3 H] thymidine and L-[3, 4, 5-3 H] leucine were obtained from Perkin Elmer (Boston, MA). .. Calyculin A, siRNA against tuberin/TSC2, control scrambled siRNA, cell lysis buffer (10X) and antibodies against p-PI3K p85 (T458)/p55 (T199), p-PDK1 (S241), p-Akt (T308), p-Akt (S473), Akt, p-FoxO1 (S256), p-GSK3β (S9), p-mTOR (S2448), p-mTOR (S2481), mTOR, p-p70 S6K (T389), p-S6 ribosomal protein (S235/236), p-4E-BP1 (T37/46), p-eIF4G (S1108), Tuberin/TSC2, p-Tuberin/TSC2 (T1462), p-AMPKα (T172), p-ACC (S79), methylated and non-methylated PP2A catalytic (PP2A C) subunit were purchased from Cell Signaling Technology (Beverly, MA). .. Antibodies against HA tag, PDK1 (PKB kinase), β-actin, cyclin D1 and HRP-conjugated secondary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

    Methylation:

    Article Title: Curcumin inhibits Akt/mTOR signaling through protein phosphatase-dependent mechanism *
    Article Snippet: [6-3 H] thymidine and L-[3, 4, 5-3 H] leucine were obtained from Perkin Elmer (Boston, MA). .. Calyculin A, siRNA against tuberin/TSC2, control scrambled siRNA, cell lysis buffer (10X) and antibodies against p-PI3K p85 (T458)/p55 (T199), p-PDK1 (S241), p-Akt (T308), p-Akt (S473), Akt, p-FoxO1 (S256), p-GSK3β (S9), p-mTOR (S2448), p-mTOR (S2481), mTOR, p-p70 S6K (T389), p-S6 ribosomal protein (S235/236), p-4E-BP1 (T37/46), p-eIF4G (S1108), Tuberin/TSC2, p-Tuberin/TSC2 (T1462), p-AMPKα (T172), p-ACC (S79), methylated and non-methylated PP2A catalytic (PP2A C) subunit were purchased from Cell Signaling Technology (Beverly, MA). .. Antibodies against HA tag, PDK1 (PKB kinase), β-actin, cyclin D1 and HRP-conjugated secondary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

    Incubation:

    Article Title: Synergistic inhibition of mesothelioma cell growth by the combination of clofarabine and resveratrol involves Nrf2 downregulation
    Article Snippet: Proteins (40 μg per lane) were separated on NuPAGE 4-12% bis-tris polyacrylamide gels (Invitrogen) and then electrophoretically transferred to Immuno-Blot PVDF membranes. .. The membranes were incubated for 2 h at room temperature with a 1:500 dilution of anti-Nrf2 (Santa Cruz Biotechnology), anti-HO-1 (Stressgen Biotechnologies), anti-p-Akt, and anti-p-Erk antibodies (Cell Signaling Technologies). .. Next, HRP-conjugated secondary antibody was applied at a dilution of 1 : 5,000 and the signal was visualized using an ECL detection kit (Santa Cruz Biotechnology).

    Article Title: Valproic acid, an inhibitor of class I histone deacetylases, reverses acquired Erlotinib-resistance of lung adenocarcinoma cells: a Connectivity Mapping analysis and an experimental study
    Article Snippet: The proteins were separated by 10% SDS-polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane (Life Technologies, Gaithersburg, MD). .. The blots were then incubated in a fresh blocking solution with an appropriate dilution of the primary antibody at 4°C for 24 h. The sources of antibodies were as follows: GAPDH mouse polyclonal antibody (Santa Cruz); p-MAPK (Thr202/Tyr204), MAPK , and p-AKT (Ser473); and AKT rabbit monoclonal antibody (Cell Signaling), Bax , Bcl-2 , cytochrome C mouse monoclonal antibody, caspase-3 rabbit polyclonal antibody (Santa Cruz). .. After the blots were extensively washed, the membranes were incubated with horseradish peroxidase-coupled secondary antibody (1:2000, Zhongshan Biotech Company, China) at 25°C for 1 h. The bands were visualized and quantified using the Image-Pro Plus 5.0 software (Media Cybernetics). p-MAPK and p-AKT band intensities were normalized to MAPK and AKT band intensities, respectively.

    other:

    Article Title: Urea-induced ROS generation causes insulin resistance in mice with chronic renal failure
    Article Snippet: Antibodies and reagents used were as follows: anti-phosphotyrosine, anti–IRS-1, anti–pS636–IRS-1 (UBI); anti-Akt, anti–S473-Akt (Cell Signaling Technology); anti–O-linked glycosylation RL-2 (Affinity Bioreagents); anti-RBP4 (Alexis Biochemical); and anti–3-nitrotyrosine (Upstate).

    Article Title: Epstein-Barr Virus Latent Membrane Protein-1 Effects on Plakoglobin, Cell Growth and Migration
    Article Snippet: Rabbit anti-pAkt (Ser473); anti-pGSK3α/β (Ser21/9) and anti-Akt were purchased from Cell Signaling.

    Blocking Assay:

    Article Title: Valproic acid, an inhibitor of class I histone deacetylases, reverses acquired Erlotinib-resistance of lung adenocarcinoma cells: a Connectivity Mapping analysis and an experimental study
    Article Snippet: The proteins were separated by 10% SDS-polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane (Life Technologies, Gaithersburg, MD). .. The blots were then incubated in a fresh blocking solution with an appropriate dilution of the primary antibody at 4°C for 24 h. The sources of antibodies were as follows: GAPDH mouse polyclonal antibody (Santa Cruz); p-MAPK (Thr202/Tyr204), MAPK , and p-AKT (Ser473); and AKT rabbit monoclonal antibody (Cell Signaling), Bax , Bcl-2 , cytochrome C mouse monoclonal antibody, caspase-3 rabbit polyclonal antibody (Santa Cruz). .. After the blots were extensively washed, the membranes were incubated with horseradish peroxidase-coupled secondary antibody (1:2000, Zhongshan Biotech Company, China) at 25°C for 1 h. The bands were visualized and quantified using the Image-Pro Plus 5.0 software (Media Cybernetics). p-MAPK and p-AKT band intensities were normalized to MAPK and AKT band intensities, respectively.

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  • 86
    Cell Signaling Technology Inc pan akt
    Consistent activation of <t>AKT/mTOR</t> pathway in the absence of TGFBI under general culture condition. To detect the effects of TGFBI on AKT, mTOR, S6K1 and 4EBP1 under general culture condition within the sets of 5ATE/5AT1 (A) and H28V/H28T7 (B), respectively,
    Pan Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pan akt/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pan akt - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc akt pan
    PI3KC2β increases MAPK and <t>Akt</t> signalling downstream of EGFR and PDGFR. (A) and (B) NIH3T3-V, -C2β-DN and -C2β-WT cells were serum-deprived overnight and stimulated for 10 min. with EGF (A) or PDGF (B) as indicated. Cell lysates were analysed by immunobloting and MAPK and Akt pathway activation was assessed with indicated phospho-specific antibodies. (C) and (D) Lysates of NIH3T3-V, -C2β-DN and -C2β-WT cells grown in 10% FCS were analysed for activated signalling molecules implicated in the cell cycle control and caveolae formation such as <t>p53,</t> Bcl2, PTEN and caveolin 1, −2. WT1, −2 and DN1, −2 indicate individual clones.
    Akt Pan, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/akt pan/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    akt pan - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc phospho akt
    Autophagy, ER stress, and <t>p-AKT</t> change after CQ (Q), Rapa (R) and TMZ (T) in different combination treatment GBM8401 cells and U87MG cells were treated with CQ, Rapa and TMZ in different combinations, harvested at 24 hours and immunoblotted for LC3-I, LC3-II and ( A ) <t>p62,</t> ( B ) p-AKT, ( C ) p-mTOR and p-S6K, ( D ) GRP78 and CHOP.
    Phospho Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho akt/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho akt - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc in vitro synip phosphorylation akt substrate phosphorylation assay kit
    <t>Synip</t> phosphorylation by Akt2. (A) Candidate <t>Akt</t> phosphorylation consensus motif in Synip is presented. (B) Either FLAG-WT-Synip or FLAG-S99F-Synip was expressed in CHOIR cells by electroporation. After 15 min of insulin stimulation, Synip was immunoprecipitated by FLAG antibody and samples were separated on SDS-PAGE. Phosphorylated Synip signal was analyzed by Akt phosphospecific substrate antibody immunoblotting. These are representative experiments independently performed three times. (C) Synthesized oligo peptides were phosphorylated as described in Materials and methods. These were repeated four to five times. Recombinant Akt2 was capable of phosphorylating the Synip peptide compared with Akt1 (*, P
    In Vitro Synip Phosphorylation Akt Substrate Phosphorylation Assay Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/in vitro synip phosphorylation akt substrate phosphorylation assay kit/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    in vitro synip phosphorylation akt substrate phosphorylation assay kit - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Consistent activation of AKT/mTOR pathway in the absence of TGFBI under general culture condition. To detect the effects of TGFBI on AKT, mTOR, S6K1 and 4EBP1 under general culture condition within the sets of 5ATE/5AT1 (A) and H28V/H28T7 (B), respectively,

    Journal: International journal of oncology

    Article Title: Transforming growth factor-?-induced protein (TGFBI) suppresses mesothelioma progression through the Akt/mTOR pathway

    doi: 10.3892/ijo.2011.1097

    Figure Lengend Snippet: Consistent activation of AKT/mTOR pathway in the absence of TGFBI under general culture condition. To detect the effects of TGFBI on AKT, mTOR, S6K1 and 4EBP1 under general culture condition within the sets of 5ATE/5AT1 (A) and H28V/H28T7 (B), respectively,

    Article Snippet: Antibodies to EGF receptor, p85, phosphor-AKT, pan-AKT, phosphor-mTOR, mTOR, phosphor-p70S6, p-70S6, phosphor-4EBP1, 4EBP1, and β-actin were obtained from Cell Signaling Technology.

    Techniques: Activation Assay

    The potentiation of EGF-induced AKT/mTOR pathway in the absence of TGFBI. To detect the effects on AKT and mTOR in response to EGF treatment in sets of 5ATE/5AT1 (A), (C) and H28V/H28T7 (B), (D), respectively, cells were serum starved for 16 h, and then

    Journal: International journal of oncology

    Article Title: Transforming growth factor-?-induced protein (TGFBI) suppresses mesothelioma progression through the Akt/mTOR pathway

    doi: 10.3892/ijo.2011.1097

    Figure Lengend Snippet: The potentiation of EGF-induced AKT/mTOR pathway in the absence of TGFBI. To detect the effects on AKT and mTOR in response to EGF treatment in sets of 5ATE/5AT1 (A), (C) and H28V/H28T7 (B), (D), respectively, cells were serum starved for 16 h, and then

    Article Snippet: Antibodies to EGF receptor, p85, phosphor-AKT, pan-AKT, phosphor-mTOR, mTOR, phosphor-p70S6, p-70S6, phosphor-4EBP1, 4EBP1, and β-actin were obtained from Cell Signaling Technology.

    Techniques:

    PI3KC2β increases MAPK and Akt signalling downstream of EGFR and PDGFR. (A) and (B) NIH3T3-V, -C2β-DN and -C2β-WT cells were serum-deprived overnight and stimulated for 10 min. with EGF (A) or PDGF (B) as indicated. Cell lysates were analysed by immunobloting and MAPK and Akt pathway activation was assessed with indicated phospho-specific antibodies. (C) and (D) Lysates of NIH3T3-V, -C2β-DN and -C2β-WT cells grown in 10% FCS were analysed for activated signalling molecules implicated in the cell cycle control and caveolae formation such as p53, Bcl2, PTEN and caveolin 1, −2. WT1, −2 and DN1, −2 indicate individual clones.

    Journal: PLoS ONE

    Article Title: Phosphoinositide 3-Kinase C2? Regulates RhoA and the Actin Cytoskeleton through an Interaction with Dbl

    doi: 10.1371/journal.pone.0044945

    Figure Lengend Snippet: PI3KC2β increases MAPK and Akt signalling downstream of EGFR and PDGFR. (A) and (B) NIH3T3-V, -C2β-DN and -C2β-WT cells were serum-deprived overnight and stimulated for 10 min. with EGF (A) or PDGF (B) as indicated. Cell lysates were analysed by immunobloting and MAPK and Akt pathway activation was assessed with indicated phospho-specific antibodies. (C) and (D) Lysates of NIH3T3-V, -C2β-DN and -C2β-WT cells grown in 10% FCS were analysed for activated signalling molecules implicated in the cell cycle control and caveolae formation such as p53, Bcl2, PTEN and caveolin 1, −2. WT1, −2 and DN1, −2 indicate individual clones.

    Article Snippet: Reagents and Antibodies The following antibodies were used: PI3KC2β was described in , 9E10 myc epitope, p115RhoGEF, Dbl, Caveolin-2, Grb2, Src, RhoA, GFP, p53 (Santa Cruz Biotechnology, Santa Cruz, CA, USA); Caveolin-1, Akt (pan), phospho-Akt (Ser473), phospho-Akt (Thr308), phospho-SAPK/JNK (Thr183/Tyr185), phospho-S6 ribosomal protein (Ser240/244), phospho-GSK-3α/β (Ser21/9), phospho-FOXO1 (Thr24)/FOXO3a (Thr32), phospho-Erk1/2 (Thr202/Tyr204), p44/42 MAPK, Bcl-2, Bcl-XL, PTEN, phospho-Bad (Ser136) (Cell Signalling Technology, Danvers, MA, USA); FITC-conjugated anti-mouse and anti-rabbit antibodies, β-actin, anti-mouse IgG (Sigma-Aldrich, St Louis, MO, USA); anti-rabbit-IgG (R & d Systems, Minneapolis, MN,USA); Rac1 (BD Transduction Laboratories, USA).

    Techniques: Western Blot, Activation Assay, Clone Assay

    Autophagy, ER stress, and p-AKT change after CQ (Q), Rapa (R) and TMZ (T) in different combination treatment GBM8401 cells and U87MG cells were treated with CQ, Rapa and TMZ in different combinations, harvested at 24 hours and immunoblotted for LC3-I, LC3-II and ( A ) p62, ( B ) p-AKT, ( C ) p-mTOR and p-S6K, ( D ) GRP78 and CHOP.

    Journal: Oncotarget

    Article Title: Temozolomide, sirolimus and chloroquine is a new therapeutic combination that synergizes to disrupt lysosomal function and cholesterol homeostasis in GBM cells

    doi: 10.18632/oncotarget.23855

    Figure Lengend Snippet: Autophagy, ER stress, and p-AKT change after CQ (Q), Rapa (R) and TMZ (T) in different combination treatment GBM8401 cells and U87MG cells were treated with CQ, Rapa and TMZ in different combinations, harvested at 24 hours and immunoblotted for LC3-I, LC3-II and ( A ) p62, ( B ) p-AKT, ( C ) p-mTOR and p-S6K, ( D ) GRP78 and CHOP.

    Article Snippet: The membrane was then incubated with antibodies against β-actin (Sigma, 1:10000), GAPDH (Sigma, 1:10000), LC3 (Novus Biologicals Inc., Littleton, CO, 1:10000), SQSTM1/p62 (MBL international, 1:1000), PARP (Cell Signaling Technologies, 1:1000), phospho-mTOR, phospho-Akt (Ser 473) (Cell Signaling Technologies, 1:1000), phospho-p70S6K (Cell Signaling Technologies, 1:1000), ABCA1 (Abcam, 1:1000), LDLR (Abcam, 1:5000), SREBP1 (BD, 1:1000), SREBP2 (BD, 1:1000), CHOP (Cell Signaling Technologies, 1:1000) and GRP78 (Cell Signaling Technologies, 1:1000) overnight at 4°C in PBS containing 0.05% Tween 20 and 5% nonfat milk, followed by incubation for 1 h at room temperature with horseradish peroxidase-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) in the same buffer.

    Techniques:

    Synip phosphorylation by Akt2. (A) Candidate Akt phosphorylation consensus motif in Synip is presented. (B) Either FLAG-WT-Synip or FLAG-S99F-Synip was expressed in CHOIR cells by electroporation. After 15 min of insulin stimulation, Synip was immunoprecipitated by FLAG antibody and samples were separated on SDS-PAGE. Phosphorylated Synip signal was analyzed by Akt phosphospecific substrate antibody immunoblotting. These are representative experiments independently performed three times. (C) Synthesized oligo peptides were phosphorylated as described in Materials and methods. These were repeated four to five times. Recombinant Akt2 was capable of phosphorylating the Synip peptide compared with Akt1 (*, P

    Journal: The Journal of Cell Biology

    Article Title: Akt2 phosphorylates Synip to regulate docking and fusion of GLUT4-containing vesicles

    doi: 10.1083/jcb.200408182

    Figure Lengend Snippet: Synip phosphorylation by Akt2. (A) Candidate Akt phosphorylation consensus motif in Synip is presented. (B) Either FLAG-WT-Synip or FLAG-S99F-Synip was expressed in CHOIR cells by electroporation. After 15 min of insulin stimulation, Synip was immunoprecipitated by FLAG antibody and samples were separated on SDS-PAGE. Phosphorylated Synip signal was analyzed by Akt phosphospecific substrate antibody immunoblotting. These are representative experiments independently performed three times. (C) Synthesized oligo peptides were phosphorylated as described in Materials and methods. These were repeated four to five times. Recombinant Akt2 was capable of phosphorylating the Synip peptide compared with Akt1 (*, P

    Article Snippet: In vitro Synip phosphorylation Akt substrate phosphorylation assay kit was purchased from Upstate Cell Signal Solutions and we followed the manufacture's procedure.

    Techniques: Electroporation, Immunoprecipitation, SDS Page, Synthesized, Recombinant