phosphorylated p src  (Cell Signaling Technology Inc)


Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc phosphorylated p src
    Effect of silencing KRT17 on FAK / SRC / ERK pathway. A Stand for the immunoblots of p-FAK, FAK, <t>p-Src,</t> Src, p-ERK, ERK, and β-actin in PANC-1 by the western blot. B The statistical plot of p-FAK, p-Src, and p-ERK protein expression levels in PANC-1. C The immunoblots of p-FAK, FAK, p-Src, Src, p-ERK, ERK, and β-actin in SW1990 by the western blot. D The statistical plot of p-FAK, p-Src, and p-ERK protein expression levels in SW1990. * P < 0.05 vs. si-NC.
    Phosphorylated P Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated p src/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated p src - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "MicroRNA-485-5p targets keratin17 to regulate pancreatic cancer cell proliferation and invasion via the FAK / SRC / ERK pathway"

    Article Title: MicroRNA-485-5p targets keratin17 to regulate pancreatic cancer cell proliferation and invasion via the FAK / SRC / ERK pathway

    Journal: Journal of Cancer

    doi: 10.7150/jca.90689

    Effect of silencing KRT17 on FAK / SRC / ERK pathway. A Stand for the immunoblots of p-FAK, FAK, p-Src, Src, p-ERK, ERK, and β-actin in PANC-1 by the western blot. B The statistical plot of p-FAK, p-Src, and p-ERK protein expression levels in PANC-1. C The immunoblots of p-FAK, FAK, p-Src, Src, p-ERK, ERK, and β-actin in SW1990 by the western blot. D The statistical plot of p-FAK, p-Src, and p-ERK protein expression levels in SW1990. * P < 0.05 vs. si-NC.
    Figure Legend Snippet: Effect of silencing KRT17 on FAK / SRC / ERK pathway. A Stand for the immunoblots of p-FAK, FAK, p-Src, Src, p-ERK, ERK, and β-actin in PANC-1 by the western blot. B The statistical plot of p-FAK, p-Src, and p-ERK protein expression levels in PANC-1. C The immunoblots of p-FAK, FAK, p-Src, Src, p-ERK, ERK, and β-actin in SW1990 by the western blot. D The statistical plot of p-FAK, p-Src, and p-ERK protein expression levels in SW1990. * P < 0.05 vs. si-NC.

    Techniques Used: Western Blot, Expressing

    phosphorylated p src  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc phosphorylated p src
    Phosphorylated P Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated p src/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated p src - by Bioz Stars, 2024-07
    86/100 stars

    Images

    phosphoryl src p src  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Cell Signaling Technology Inc phosphoryl src p src
    LicA inhibits metastasis via the <t>FAK/Src</t> signaling pathway of RCC cells. Different LicA concentrations (0, 6.25, 12.5, and 12.5 µM) were applied to ACHN cells, and the cells were then left to react for 24 h. ( A ) Western blotting was utilized to examine the expression of p-FAK, t-FAK, <t>p-Src,</t> and t-Src in cell lysates. β-actin served as a internal control. The ACHN cells were treated with 12.5 µM LicA in the presence or absence of ( B ) a FAK inhibitor (PF) or ( C ) an Src inhibitor (PP2) for 24 h. The cell lysates were examined using Western blotting to measure the protein levels of p-FAK, t-FAK, and LC3B. ( D , E ) Next, migration and Matrigel-invasion assays were used to assess migration and invasion. Data represent the mean ± SD of at least three separate experiments. ** p < 0.01, compared to un-treated control (0 µM); # p < 0.05, compared with LicA-treated alone.
    Phosphoryl Src P Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphoryl src p src/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphoryl src p src - by Bioz Stars, 2024-07
    96/100 stars

    Images

    1) Product Images from "Licochalcone A Suppresses Renal Cancer Cell Proliferation and Metastasis by Engagement of Sp1-Mediated LC3 Expression"

    Article Title: Licochalcone A Suppresses Renal Cancer Cell Proliferation and Metastasis by Engagement of Sp1-Mediated LC3 Expression

    Journal: Pharmaceutics

    doi: 10.3390/pharmaceutics15020684

    LicA inhibits metastasis via the FAK/Src signaling pathway of RCC cells. Different LicA concentrations (0, 6.25, 12.5, and 12.5 µM) were applied to ACHN cells, and the cells were then left to react for 24 h. ( A ) Western blotting was utilized to examine the expression of p-FAK, t-FAK, p-Src, and t-Src in cell lysates. β-actin served as a internal control. The ACHN cells were treated with 12.5 µM LicA in the presence or absence of ( B ) a FAK inhibitor (PF) or ( C ) an Src inhibitor (PP2) for 24 h. The cell lysates were examined using Western blotting to measure the protein levels of p-FAK, t-FAK, and LC3B. ( D , E ) Next, migration and Matrigel-invasion assays were used to assess migration and invasion. Data represent the mean ± SD of at least three separate experiments. ** p < 0.01, compared to un-treated control (0 µM); # p < 0.05, compared with LicA-treated alone.
    Figure Legend Snippet: LicA inhibits metastasis via the FAK/Src signaling pathway of RCC cells. Different LicA concentrations (0, 6.25, 12.5, and 12.5 µM) were applied to ACHN cells, and the cells were then left to react for 24 h. ( A ) Western blotting was utilized to examine the expression of p-FAK, t-FAK, p-Src, and t-Src in cell lysates. β-actin served as a internal control. The ACHN cells were treated with 12.5 µM LicA in the presence or absence of ( B ) a FAK inhibitor (PF) or ( C ) an Src inhibitor (PP2) for 24 h. The cell lysates were examined using Western blotting to measure the protein levels of p-FAK, t-FAK, and LC3B. ( D , E ) Next, migration and Matrigel-invasion assays were used to assess migration and invasion. Data represent the mean ± SD of at least three separate experiments. ** p < 0.01, compared to un-treated control (0 µM); # p < 0.05, compared with LicA-treated alone.

    Techniques Used: Western Blot, Expressing, Migration

    LicA inhibits RCC tumorigenesis and metastasis via the downregulation of the phosphoryl-Src/-FAK pathway. It does so by decreasing the Sp1 transcription activity, resulting in suppressed LC3B expression.
    Figure Legend Snippet: LicA inhibits RCC tumorigenesis and metastasis via the downregulation of the phosphoryl-Src/-FAK pathway. It does so by decreasing the Sp1 transcription activity, resulting in suppressed LC3B expression.

    Techniques Used: Activity Assay, Expressing

    anti phosphorylated p src  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc anti phosphorylated p src
    Anti Phosphorylated P Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phosphorylated p src/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti phosphorylated p src - by Bioz Stars, 2024-07
    86/100 stars

    Images

    anti phosphorylated p src  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc anti phosphorylated p src
    Anti Phosphorylated P Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phosphorylated p src/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti phosphorylated p src - by Bioz Stars, 2024-07
    86/100 stars

    Images

    phosphorylated src p src  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc phosphorylated src p src
    Suppression of <t>Src,</t> FAK, and ERK1/2 signaling pathways by antcin-H. (a) Time course-dependent experiment. 786-0 cells were treated without or with 100 μ M antcin-H for 4, 8, and 24 h. (b) Dose-dependent experiment. Cells were treated without or with 20, 50, and 100 μ M antcin-H for 24 h. After incubation, total protein lysates were isolated; the Western blotting analysis was performed to examine the levels <t>of</t> <t>phosphorylated-Src,</t> phosphorylated-FAK, phosphorylated-ERK1/2, phosphorylated-C/EBP- β , and c-Fos. β -Actin was used as an internal loading control.
    Phosphorylated Src P Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated src p src/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated src p src - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Antcin-H Isolated from Antrodia cinnamomea Inhibits Renal Cancer Cell Invasion Partly through Inactivation of FAK-ERK-C/EBP- β /c-Fos-MMP-7 Pathways"

    Article Title: Antcin-H Isolated from Antrodia cinnamomea Inhibits Renal Cancer Cell Invasion Partly through Inactivation of FAK-ERK-C/EBP- β /c-Fos-MMP-7 Pathways

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2017/5052870

    Suppression of Src, FAK, and ERK1/2 signaling pathways by antcin-H. (a) Time course-dependent experiment. 786-0 cells were treated without or with 100 μ M antcin-H for 4, 8, and 24 h. (b) Dose-dependent experiment. Cells were treated without or with 20, 50, and 100 μ M antcin-H for 24 h. After incubation, total protein lysates were isolated; the Western blotting analysis was performed to examine the levels of phosphorylated-Src, phosphorylated-FAK, phosphorylated-ERK1/2, phosphorylated-C/EBP- β , and c-Fos. β -Actin was used as an internal loading control.
    Figure Legend Snippet: Suppression of Src, FAK, and ERK1/2 signaling pathways by antcin-H. (a) Time course-dependent experiment. 786-0 cells were treated without or with 100 μ M antcin-H for 4, 8, and 24 h. (b) Dose-dependent experiment. Cells were treated without or with 20, 50, and 100 μ M antcin-H for 24 h. After incubation, total protein lysates were isolated; the Western blotting analysis was performed to examine the levels of phosphorylated-Src, phosphorylated-FAK, phosphorylated-ERK1/2, phosphorylated-C/EBP- β , and c-Fos. β -Actin was used as an internal loading control.

    Techniques Used: Incubation, Isolation, Western Blot

    Schematic model of the proposed signaling pathways involved in suppressing cell migration and invasion by antcin-H in human RCC 786-0 cells. Antcin-H inhibits Src, FAK, and ERK1/2 phosphorylated activation, in turn decreasing paxillin, c-Fos, and C/EBP- β activities, reducing the binding of c-Fos and phosphorylated-C/EBP- β to AP-1 and C/EBP- β response elements, thereby decreasing MMPs gene expression, especially MMP-7. Downregulation of MMP-7 and upregulation of TIMP-3 and TIMP-4 gene expression block the degradation of the extracellular matrix proteins and impair the cell invasion. Besides, reducing paxillin phosphorylation and vimentin expression prevents 786-0 cell motility.
    Figure Legend Snippet: Schematic model of the proposed signaling pathways involved in suppressing cell migration and invasion by antcin-H in human RCC 786-0 cells. Antcin-H inhibits Src, FAK, and ERK1/2 phosphorylated activation, in turn decreasing paxillin, c-Fos, and C/EBP- β activities, reducing the binding of c-Fos and phosphorylated-C/EBP- β to AP-1 and C/EBP- β response elements, thereby decreasing MMPs gene expression, especially MMP-7. Downregulation of MMP-7 and upregulation of TIMP-3 and TIMP-4 gene expression block the degradation of the extracellular matrix proteins and impair the cell invasion. Besides, reducing paxillin phosphorylation and vimentin expression prevents 786-0 cell motility.

    Techniques Used: Migration, Activation Assay, Binding Assay, Expressing, Blocking Assay


    Structured Review

    Santa Cruz Biotechnology phosphorylated c src
    Phosphorylated C Src, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated c src/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated c src - by Bioz Stars, 2024-07
    93/100 stars

    Images


    Structured Review

    Santa Cruz Biotechnology phosphorylated c src
    Phosphorylated C Src, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated c src/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated c src - by Bioz Stars, 2024-07
    93/100 stars

    Images


    Structured Review

    GeneTex phosphorylated src p src
    Ectopic expression of Hic-5 significantly enhanced the activation of <t>Src,</t> AKT and JNK for the cell migration of TFK1. TFK1 cells were treated with dasatinib (Dasa) at indicated concentrations ( a ) or transfected with Hic-5 expression plasmid (p-Hic-5) ( b – d ). Western blot of indicated molecules ( a – c ) and transwell migration assay of TFK1 ( d ) were performed. In ( b , c ), the Abs used for detecting p-Hic-5 encoded protein (Hic-5–GFP fusion protein) were Hic-5 and GFP, respectively, revealing the same molecular weight. In ( d ), the crystal-violet-stained, migrated cells were pictured under 40× magnification. ( e ) is the quantitative data of ( d ) using ImageJ software. Relative motility was calculated taking the data of the p-CMV vector as 1.0. The data shown are representative of two reproducible results.
    Phosphorylated Src P Src, supplied by GeneTex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated src p src/product/GeneTex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated src p src - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Suppressing of Src–Hic-5–JNK–AKT Signaling Reduced GAPDH Expression for Preventing the Progression of HuCCT1 Cholangiocarcinoma"

    Article Title: Suppressing of Src–Hic-5–JNK–AKT Signaling Reduced GAPDH Expression for Preventing the Progression of HuCCT1 Cholangiocarcinoma

    Journal: Pharmaceutics

    doi: 10.3390/pharmaceutics14122698

    Ectopic expression of Hic-5 significantly enhanced the activation of Src, AKT and JNK for the cell migration of TFK1. TFK1 cells were treated with dasatinib (Dasa) at indicated concentrations ( a ) or transfected with Hic-5 expression plasmid (p-Hic-5) ( b – d ). Western blot of indicated molecules ( a – c ) and transwell migration assay of TFK1 ( d ) were performed. In ( b , c ), the Abs used for detecting p-Hic-5 encoded protein (Hic-5–GFP fusion protein) were Hic-5 and GFP, respectively, revealing the same molecular weight. In ( d ), the crystal-violet-stained, migrated cells were pictured under 40× magnification. ( e ) is the quantitative data of ( d ) using ImageJ software. Relative motility was calculated taking the data of the p-CMV vector as 1.0. The data shown are representative of two reproducible results.
    Figure Legend Snippet: Ectopic expression of Hic-5 significantly enhanced the activation of Src, AKT and JNK for the cell migration of TFK1. TFK1 cells were treated with dasatinib (Dasa) at indicated concentrations ( a ) or transfected with Hic-5 expression plasmid (p-Hic-5) ( b – d ). Western blot of indicated molecules ( a – c ) and transwell migration assay of TFK1 ( d ) were performed. In ( b , c ), the Abs used for detecting p-Hic-5 encoded protein (Hic-5–GFP fusion protein) were Hic-5 and GFP, respectively, revealing the same molecular weight. In ( d ), the crystal-violet-stained, migrated cells were pictured under 40× magnification. ( e ) is the quantitative data of ( d ) using ImageJ software. Relative motility was calculated taking the data of the p-CMV vector as 1.0. The data shown are representative of two reproducible results.

    Techniques Used: Expressing, Activation Assay, Migration, Transfection, Plasmid Preparation, Western Blot, Transwell Migration Assay, Molecular Weight, Staining, Software

    phosphorylated forms p src tyr416  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc phosphorylated forms p src tyr416
    Phosphorylated Forms P Src Tyr416, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated forms p src tyr416/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated forms p src tyr416 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Cell Signaling Technology Inc phosphorylated p src
    Effect of silencing KRT17 on FAK / SRC / ERK pathway. A Stand for the immunoblots of p-FAK, FAK, <t>p-Src,</t> Src, p-ERK, ERK, and β-actin in PANC-1 by the western blot. B The statistical plot of p-FAK, p-Src, and p-ERK protein expression levels in PANC-1. C The immunoblots of p-FAK, FAK, p-Src, Src, p-ERK, ERK, and β-actin in SW1990 by the western blot. D The statistical plot of p-FAK, p-Src, and p-ERK protein expression levels in SW1990. * P < 0.05 vs. si-NC.
    Phosphorylated P Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated p src/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated p src - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc phosphoryl src p src
    LicA inhibits metastasis via the <t>FAK/Src</t> signaling pathway of RCC cells. Different LicA concentrations (0, 6.25, 12.5, and 12.5 µM) were applied to ACHN cells, and the cells were then left to react for 24 h. ( A ) Western blotting was utilized to examine the expression of p-FAK, t-FAK, <t>p-Src,</t> and t-Src in cell lysates. β-actin served as a internal control. The ACHN cells were treated with 12.5 µM LicA in the presence or absence of ( B ) a FAK inhibitor (PF) or ( C ) an Src inhibitor (PP2) for 24 h. The cell lysates were examined using Western blotting to measure the protein levels of p-FAK, t-FAK, and LC3B. ( D , E ) Next, migration and Matrigel-invasion assays were used to assess migration and invasion. Data represent the mean ± SD of at least three separate experiments. ** p < 0.01, compared to un-treated control (0 µM); # p < 0.05, compared with LicA-treated alone.
    Phosphoryl Src P Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphoryl src p src/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphoryl src p src - by Bioz Stars, 2024-07
    96/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc anti phosphorylated p src
    LicA inhibits metastasis via the <t>FAK/Src</t> signaling pathway of RCC cells. Different LicA concentrations (0, 6.25, 12.5, and 12.5 µM) were applied to ACHN cells, and the cells were then left to react for 24 h. ( A ) Western blotting was utilized to examine the expression of p-FAK, t-FAK, <t>p-Src,</t> and t-Src in cell lysates. β-actin served as a internal control. The ACHN cells were treated with 12.5 µM LicA in the presence or absence of ( B ) a FAK inhibitor (PF) or ( C ) an Src inhibitor (PP2) for 24 h. The cell lysates were examined using Western blotting to measure the protein levels of p-FAK, t-FAK, and LC3B. ( D , E ) Next, migration and Matrigel-invasion assays were used to assess migration and invasion. Data represent the mean ± SD of at least three separate experiments. ** p < 0.01, compared to un-treated control (0 µM); # p < 0.05, compared with LicA-treated alone.
    Anti Phosphorylated P Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phosphorylated p src/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti phosphorylated p src - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc phosphorylated src p src
    Suppression of <t>Src,</t> FAK, and ERK1/2 signaling pathways by antcin-H. (a) Time course-dependent experiment. 786-0 cells were treated without or with 100 μ M antcin-H for 4, 8, and 24 h. (b) Dose-dependent experiment. Cells were treated without or with 20, 50, and 100 μ M antcin-H for 24 h. After incubation, total protein lysates were isolated; the Western blotting analysis was performed to examine the levels <t>of</t> <t>phosphorylated-Src,</t> phosphorylated-FAK, phosphorylated-ERK1/2, phosphorylated-C/EBP- β , and c-Fos. β -Actin was used as an internal loading control.
    Phosphorylated Src P Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated src p src/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated src p src - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    93
    Santa Cruz Biotechnology phosphorylated c src
    Suppression of <t>Src,</t> FAK, and ERK1/2 signaling pathways by antcin-H. (a) Time course-dependent experiment. 786-0 cells were treated without or with 100 μ M antcin-H for 4, 8, and 24 h. (b) Dose-dependent experiment. Cells were treated without or with 20, 50, and 100 μ M antcin-H for 24 h. After incubation, total protein lysates were isolated; the Western blotting analysis was performed to examine the levels <t>of</t> <t>phosphorylated-Src,</t> phosphorylated-FAK, phosphorylated-ERK1/2, phosphorylated-C/EBP- β , and c-Fos. β -Actin was used as an internal loading control.
    Phosphorylated C Src, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated c src/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated c src - by Bioz Stars, 2024-07
    93/100 stars
      Buy from Supplier

    86
    GeneTex phosphorylated src p src
    Ectopic expression of Hic-5 significantly enhanced the activation of <t>Src,</t> AKT and JNK for the cell migration of TFK1. TFK1 cells were treated with dasatinib (Dasa) at indicated concentrations ( a ) or transfected with Hic-5 expression plasmid (p-Hic-5) ( b – d ). Western blot of indicated molecules ( a – c ) and transwell migration assay of TFK1 ( d ) were performed. In ( b , c ), the Abs used for detecting p-Hic-5 encoded protein (Hic-5–GFP fusion protein) were Hic-5 and GFP, respectively, revealing the same molecular weight. In ( d ), the crystal-violet-stained, migrated cells were pictured under 40× magnification. ( e ) is the quantitative data of ( d ) using ImageJ software. Relative motility was calculated taking the data of the p-CMV vector as 1.0. The data shown are representative of two reproducible results.
    Phosphorylated Src P Src, supplied by GeneTex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated src p src/product/GeneTex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated src p src - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc phosphorylated forms p src tyr416
    Ectopic expression of Hic-5 significantly enhanced the activation of <t>Src,</t> AKT and JNK for the cell migration of TFK1. TFK1 cells were treated with dasatinib (Dasa) at indicated concentrations ( a ) or transfected with Hic-5 expression plasmid (p-Hic-5) ( b – d ). Western blot of indicated molecules ( a – c ) and transwell migration assay of TFK1 ( d ) were performed. In ( b , c ), the Abs used for detecting p-Hic-5 encoded protein (Hic-5–GFP fusion protein) were Hic-5 and GFP, respectively, revealing the same molecular weight. In ( d ), the crystal-violet-stained, migrated cells were pictured under 40× magnification. ( e ) is the quantitative data of ( d ) using ImageJ software. Relative motility was calculated taking the data of the p-CMV vector as 1.0. The data shown are representative of two reproducible results.
    Phosphorylated Forms P Src Tyr416, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated forms p src tyr416/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated forms p src tyr416 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    Effect of silencing KRT17 on FAK / SRC / ERK pathway. A Stand for the immunoblots of p-FAK, FAK, p-Src, Src, p-ERK, ERK, and β-actin in PANC-1 by the western blot. B The statistical plot of p-FAK, p-Src, and p-ERK protein expression levels in PANC-1. C The immunoblots of p-FAK, FAK, p-Src, Src, p-ERK, ERK, and β-actin in SW1990 by the western blot. D The statistical plot of p-FAK, p-Src, and p-ERK protein expression levels in SW1990. * P < 0.05 vs. si-NC.

    Journal: Journal of Cancer

    Article Title: MicroRNA-485-5p targets keratin17 to regulate pancreatic cancer cell proliferation and invasion via the FAK / SRC / ERK pathway

    doi: 10.7150/jca.90689

    Figure Lengend Snippet: Effect of silencing KRT17 on FAK / SRC / ERK pathway. A Stand for the immunoblots of p-FAK, FAK, p-Src, Src, p-ERK, ERK, and β-actin in PANC-1 by the western blot. B The statistical plot of p-FAK, p-Src, and p-ERK protein expression levels in PANC-1. C The immunoblots of p-FAK, FAK, p-Src, Src, p-ERK, ERK, and β-actin in SW1990 by the western blot. D The statistical plot of p-FAK, p-Src, and p-ERK protein expression levels in SW1990. * P < 0.05 vs. si-NC.

    Article Snippet: Note the inner tank electrophoresis liquid needs to be filled, the outer tank is over 5 cm from the bottom; After loading, the connected electrophoresis instrument power supply, set the instrument parameters, the concentrator was 80 V, and the separating gum was 120 v. After electrophoresis with loading buffer to the bottom of the gel, stop electrophoresis, turn off the power supply: cut a PVDF membrane of suitable size according to the gel size and immerse it with methanol for 10 min; Gently disassemble the glass plates and remove the gel; Multi empty pads, thick filter paper, gel (black plate), PVDF membrane (white version) were placed on the splint in sequence according to the "black glue white membrane", and the splint constructed from the "sandwich structure" was inserted into the transmembrane tank at a constant current of 240 MMA for 120 min; The PVDF membrane was removed from the splint and placed into an antibody incubation tank containing Western blocking buffer; Tbst buffer wash 5 times, 5 min / time, the antibodies against KRT17, cyclinD1, CDK1, CDK2, phosphorylated (p)-FAK, FAK, phosphorylated (p)-Src, Src, phosphorylated (p)-ERK, ERK, and β-actin (Cell Signaling technology, Inc.) were poured into the antibody incubation tank, respectively, and the refrigerator at 4 ℃ overnight: tbst buffer wash 5 times, 5 min / time; Diluted secondary antibody (1:3000; Abcam, Cambridge, Ma, USA) working solution was added into the incubation tank for 3-4h at room temperature shaker; Wash 4 times with tbst buffer, 5 min / time; Luminescent detection solution was configured in a 1:1 ratio; Forceps after removing the membrane and blotting the excess liquid on the membrane, luminescence detection solution mixed with PVDF membrane, and placed it into a phosphorimager and imaged (BioRad).

    Techniques: Western Blot, Expressing

    LicA inhibits metastasis via the FAK/Src signaling pathway of RCC cells. Different LicA concentrations (0, 6.25, 12.5, and 12.5 µM) were applied to ACHN cells, and the cells were then left to react for 24 h. ( A ) Western blotting was utilized to examine the expression of p-FAK, t-FAK, p-Src, and t-Src in cell lysates. β-actin served as a internal control. The ACHN cells were treated with 12.5 µM LicA in the presence or absence of ( B ) a FAK inhibitor (PF) or ( C ) an Src inhibitor (PP2) for 24 h. The cell lysates were examined using Western blotting to measure the protein levels of p-FAK, t-FAK, and LC3B. ( D , E ) Next, migration and Matrigel-invasion assays were used to assess migration and invasion. Data represent the mean ± SD of at least three separate experiments. ** p < 0.01, compared to un-treated control (0 µM); # p < 0.05, compared with LicA-treated alone.

    Journal: Pharmaceutics

    Article Title: Licochalcone A Suppresses Renal Cancer Cell Proliferation and Metastasis by Engagement of Sp1-Mediated LC3 Expression

    doi: 10.3390/pharmaceutics15020684

    Figure Lengend Snippet: LicA inhibits metastasis via the FAK/Src signaling pathway of RCC cells. Different LicA concentrations (0, 6.25, 12.5, and 12.5 µM) were applied to ACHN cells, and the cells were then left to react for 24 h. ( A ) Western blotting was utilized to examine the expression of p-FAK, t-FAK, p-Src, and t-Src in cell lysates. β-actin served as a internal control. The ACHN cells were treated with 12.5 µM LicA in the presence or absence of ( B ) a FAK inhibitor (PF) or ( C ) an Src inhibitor (PP2) for 24 h. The cell lysates were examined using Western blotting to measure the protein levels of p-FAK, t-FAK, and LC3B. ( D , E ) Next, migration and Matrigel-invasion assays were used to assess migration and invasion. Data represent the mean ± SD of at least three separate experiments. ** p < 0.01, compared to un-treated control (0 µM); # p < 0.05, compared with LicA-treated alone.

    Article Snippet: Anti-phosphoryl-FAK (p-FAK), total-FAK (t-FAK), phosphoryl-Src (p-Src) and total-Src (t-Src) antibodies were purchased from Cell Signaling (Danvers, MA, USA).

    Techniques: Western Blot, Expressing, Migration

    LicA inhibits RCC tumorigenesis and metastasis via the downregulation of the phosphoryl-Src/-FAK pathway. It does so by decreasing the Sp1 transcription activity, resulting in suppressed LC3B expression.

    Journal: Pharmaceutics

    Article Title: Licochalcone A Suppresses Renal Cancer Cell Proliferation and Metastasis by Engagement of Sp1-Mediated LC3 Expression

    doi: 10.3390/pharmaceutics15020684

    Figure Lengend Snippet: LicA inhibits RCC tumorigenesis and metastasis via the downregulation of the phosphoryl-Src/-FAK pathway. It does so by decreasing the Sp1 transcription activity, resulting in suppressed LC3B expression.

    Article Snippet: Anti-phosphoryl-FAK (p-FAK), total-FAK (t-FAK), phosphoryl-Src (p-Src) and total-Src (t-Src) antibodies were purchased from Cell Signaling (Danvers, MA, USA).

    Techniques: Activity Assay, Expressing

    Suppression of Src, FAK, and ERK1/2 signaling pathways by antcin-H. (a) Time course-dependent experiment. 786-0 cells were treated without or with 100 μ M antcin-H for 4, 8, and 24 h. (b) Dose-dependent experiment. Cells were treated without or with 20, 50, and 100 μ M antcin-H for 24 h. After incubation, total protein lysates were isolated; the Western blotting analysis was performed to examine the levels of phosphorylated-Src, phosphorylated-FAK, phosphorylated-ERK1/2, phosphorylated-C/EBP- β , and c-Fos. β -Actin was used as an internal loading control.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Antcin-H Isolated from Antrodia cinnamomea Inhibits Renal Cancer Cell Invasion Partly through Inactivation of FAK-ERK-C/EBP- β /c-Fos-MMP-7 Pathways

    doi: 10.1155/2017/5052870

    Figure Lengend Snippet: Suppression of Src, FAK, and ERK1/2 signaling pathways by antcin-H. (a) Time course-dependent experiment. 786-0 cells were treated without or with 100 μ M antcin-H for 4, 8, and 24 h. (b) Dose-dependent experiment. Cells were treated without or with 20, 50, and 100 μ M antcin-H for 24 h. After incubation, total protein lysates were isolated; the Western blotting analysis was performed to examine the levels of phosphorylated-Src, phosphorylated-FAK, phosphorylated-ERK1/2, phosphorylated-C/EBP- β , and c-Fos. β -Actin was used as an internal loading control.

    Article Snippet: Immunoblotting was performed using primary antibodies against Src, phosphorylated-Src (p-Src), paxillin, and phosphorylated-paxillin (p-PXN) (Cell Signaling Technology, Beverly, MA) and ERK1/2, phosphorylated-ERK1/2 (p-ERK1/2), c-Fos, C/EBP- β , phosphorylated-C/EBP- β (p-C/EBP- β ), FAK, and phosphorylated-FAK (p-FAK) (Santa Cruz, CA, USA).

    Techniques: Incubation, Isolation, Western Blot

    Schematic model of the proposed signaling pathways involved in suppressing cell migration and invasion by antcin-H in human RCC 786-0 cells. Antcin-H inhibits Src, FAK, and ERK1/2 phosphorylated activation, in turn decreasing paxillin, c-Fos, and C/EBP- β activities, reducing the binding of c-Fos and phosphorylated-C/EBP- β to AP-1 and C/EBP- β response elements, thereby decreasing MMPs gene expression, especially MMP-7. Downregulation of MMP-7 and upregulation of TIMP-3 and TIMP-4 gene expression block the degradation of the extracellular matrix proteins and impair the cell invasion. Besides, reducing paxillin phosphorylation and vimentin expression prevents 786-0 cell motility.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Antcin-H Isolated from Antrodia cinnamomea Inhibits Renal Cancer Cell Invasion Partly through Inactivation of FAK-ERK-C/EBP- β /c-Fos-MMP-7 Pathways

    doi: 10.1155/2017/5052870

    Figure Lengend Snippet: Schematic model of the proposed signaling pathways involved in suppressing cell migration and invasion by antcin-H in human RCC 786-0 cells. Antcin-H inhibits Src, FAK, and ERK1/2 phosphorylated activation, in turn decreasing paxillin, c-Fos, and C/EBP- β activities, reducing the binding of c-Fos and phosphorylated-C/EBP- β to AP-1 and C/EBP- β response elements, thereby decreasing MMPs gene expression, especially MMP-7. Downregulation of MMP-7 and upregulation of TIMP-3 and TIMP-4 gene expression block the degradation of the extracellular matrix proteins and impair the cell invasion. Besides, reducing paxillin phosphorylation and vimentin expression prevents 786-0 cell motility.

    Article Snippet: Immunoblotting was performed using primary antibodies against Src, phosphorylated-Src (p-Src), paxillin, and phosphorylated-paxillin (p-PXN) (Cell Signaling Technology, Beverly, MA) and ERK1/2, phosphorylated-ERK1/2 (p-ERK1/2), c-Fos, C/EBP- β , phosphorylated-C/EBP- β (p-C/EBP- β ), FAK, and phosphorylated-FAK (p-FAK) (Santa Cruz, CA, USA).

    Techniques: Migration, Activation Assay, Binding Assay, Expressing, Blocking Assay

    Ectopic expression of Hic-5 significantly enhanced the activation of Src, AKT and JNK for the cell migration of TFK1. TFK1 cells were treated with dasatinib (Dasa) at indicated concentrations ( a ) or transfected with Hic-5 expression plasmid (p-Hic-5) ( b – d ). Western blot of indicated molecules ( a – c ) and transwell migration assay of TFK1 ( d ) were performed. In ( b , c ), the Abs used for detecting p-Hic-5 encoded protein (Hic-5–GFP fusion protein) were Hic-5 and GFP, respectively, revealing the same molecular weight. In ( d ), the crystal-violet-stained, migrated cells were pictured under 40× magnification. ( e ) is the quantitative data of ( d ) using ImageJ software. Relative motility was calculated taking the data of the p-CMV vector as 1.0. The data shown are representative of two reproducible results.

    Journal: Pharmaceutics

    Article Title: Suppressing of Src–Hic-5–JNK–AKT Signaling Reduced GAPDH Expression for Preventing the Progression of HuCCT1 Cholangiocarcinoma

    doi: 10.3390/pharmaceutics14122698

    Figure Lengend Snippet: Ectopic expression of Hic-5 significantly enhanced the activation of Src, AKT and JNK for the cell migration of TFK1. TFK1 cells were treated with dasatinib (Dasa) at indicated concentrations ( a ) or transfected with Hic-5 expression plasmid (p-Hic-5) ( b – d ). Western blot of indicated molecules ( a – c ) and transwell migration assay of TFK1 ( d ) were performed. In ( b , c ), the Abs used for detecting p-Hic-5 encoded protein (Hic-5–GFP fusion protein) were Hic-5 and GFP, respectively, revealing the same molecular weight. In ( d ), the crystal-violet-stained, migrated cells were pictured under 40× magnification. ( e ) is the quantitative data of ( d ) using ImageJ software. Relative motility was calculated taking the data of the p-CMV vector as 1.0. The data shown are representative of two reproducible results.

    Article Snippet: Rabbit polyclonal antibodies of Hic-5, phosphorylated Src (p-Src), and AKT (p-AKT) and Ki67 were purchased from GeneTex (Irvine, CA, USA), whereas mouse monoclonal antibodies of EGFR, c-Met, Her2, Her3, phosphorylated JNK (p-JNK) and rabbit polyclonal antibody of c-Src were from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).

    Techniques: Expressing, Activation Assay, Migration, Transfection, Plasmid Preparation, Western Blot, Transwell Migration Assay, Molecular Weight, Staining, Software