phospho s1pr1  (Abcam)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Name:
    Anti EDG1 antibody EPR4538 2
    Description:

    Catalog Number:
    AB125074
    Price:
    None
    Buy from Supplier


    Structured Review

    Abcam phospho s1pr1
    Newly Generated DGCs Highly Express <t>S1PR1</t> (A) (Top) Procedure for RNA-seq analysis of new neurons 1 day after differentiating from adult hippocampal neural stem cells. (Bottom) Expression profiling of sphingolipid signaling-related genes. Mann-Whitney U test, SPHK1 p = 0.064, S1PR1 p = 0.064, ACSL1 p = 0.064, ASAH1 p = 0.064, SMPD1 p = 0.064, DGAT1 p = 0.064, ACSS2 p = 0.355, SGPL1 p = 0.064, ALDH3A2 p = 0.04, SPNS2 p = 1.000, SGMS1 p = 0.064, S1PR2 p = 0.165, CERS2 p = 0.064, CERS5 p = 0.355, CERK p = 0.355, SPHK2 p = 0.064, SPTLC1 p = 1.000, FAR1 p = 0.064, ASAH2 p = 0.643, DEGS1 p = 0.060, RAC p = 0.165, CERS4 p = 0.064, FHL2 p =1.000, UGCG p = 0.06, ACER2 p = 0.643. (B) (Left) Region of dentate gyrus dissected. (Right) S1PR isoform mRNA detection (S1PR5 was not detected). Scale bar, 100 μm. ). (D) (Top) Retroviral GFP was delivered to the DG to label newly born DGCs. (Bottom) Representative image showing expression of S1PR1 in a DGC at 7 dpi. Scale bar, 10 μm. (E) Schematic of hippocampal/cortical dissection and lipidomics analysis. (F) Concentrations of various sphingolipid metabolites in several brain regions. DG versus Ctx: p

    https://www.bioz.com/result/phospho s1pr1/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho s1pr1 - by Bioz Stars, 2021-05
    93/100 stars

    Images

    1) Product Images from "Circuit Integration Initiation of New Hippocampal Neurons in the Adult Brain"

    Article Title: Circuit Integration Initiation of New Hippocampal Neurons in the Adult Brain

    Journal: Cell reports

    doi: 10.1016/j.celrep.2019.12.084

    Newly Generated DGCs Highly Express S1PR1 (A) (Top) Procedure for RNA-seq analysis of new neurons 1 day after differentiating from adult hippocampal neural stem cells. (Bottom) Expression profiling of sphingolipid signaling-related genes. Mann-Whitney U test, SPHK1 p = 0.064, S1PR1 p = 0.064, ACSL1 p = 0.064, ASAH1 p = 0.064, SMPD1 p = 0.064, DGAT1 p = 0.064, ACSS2 p = 0.355, SGPL1 p = 0.064, ALDH3A2 p = 0.04, SPNS2 p = 1.000, SGMS1 p = 0.064, S1PR2 p = 0.165, CERS2 p = 0.064, CERS5 p = 0.355, CERK p = 0.355, SPHK2 p = 0.064, SPTLC1 p = 1.000, FAR1 p = 0.064, ASAH2 p = 0.643, DEGS1 p = 0.060, RAC p = 0.165, CERS4 p = 0.064, FHL2 p =1.000, UGCG p = 0.06, ACER2 p = 0.643. (B) (Left) Region of dentate gyrus dissected. (Right) S1PR isoform mRNA detection (S1PR5 was not detected). Scale bar, 100 μm. ). (D) (Top) Retroviral GFP was delivered to the DG to label newly born DGCs. (Bottom) Representative image showing expression of S1PR1 in a DGC at 7 dpi. Scale bar, 10 μm. (E) Schematic of hippocampal/cortical dissection and lipidomics analysis. (F) Concentrations of various sphingolipid metabolites in several brain regions. DG versus Ctx: p
    Figure Legend Snippet: Newly Generated DGCs Highly Express S1PR1 (A) (Top) Procedure for RNA-seq analysis of new neurons 1 day after differentiating from adult hippocampal neural stem cells. (Bottom) Expression profiling of sphingolipid signaling-related genes. Mann-Whitney U test, SPHK1 p = 0.064, S1PR1 p = 0.064, ACSL1 p = 0.064, ASAH1 p = 0.064, SMPD1 p = 0.064, DGAT1 p = 0.064, ACSS2 p = 0.355, SGPL1 p = 0.064, ALDH3A2 p = 0.04, SPNS2 p = 1.000, SGMS1 p = 0.064, S1PR2 p = 0.165, CERS2 p = 0.064, CERS5 p = 0.355, CERK p = 0.355, SPHK2 p = 0.064, SPTLC1 p = 1.000, FAR1 p = 0.064, ASAH2 p = 0.643, DEGS1 p = 0.060, RAC p = 0.165, CERS4 p = 0.064, FHL2 p =1.000, UGCG p = 0.06, ACER2 p = 0.643. (B) (Left) Region of dentate gyrus dissected. (Right) S1PR isoform mRNA detection (S1PR5 was not detected). Scale bar, 100 μm. ). (D) (Top) Retroviral GFP was delivered to the DG to label newly born DGCs. (Bottom) Representative image showing expression of S1PR1 in a DGC at 7 dpi. Scale bar, 10 μm. (E) Schematic of hippocampal/cortical dissection and lipidomics analysis. (F) Concentrations of various sphingolipid metabolites in several brain regions. DG versus Ctx: p

    Techniques Used: Generated, RNA Sequencing Assay, Expressing, MANN-WHITNEY, Dissection

    S1PR1 Knockdown Reduces Neurite Growth and Horizontal-to-Radial Repositioning (A) Description of shRNA expression vector and experimental timeline of shRNA viral injection and doxycycline induction. (B) (Left) Representative images of S1PR1 expression in shS1PR1 + and shLuc + DGCs at 5 dpi. (Right) Summary plot of the S1PR1 fluorescent signal intensity. *p
    Figure Legend Snippet: S1PR1 Knockdown Reduces Neurite Growth and Horizontal-to-Radial Repositioning (A) Description of shRNA expression vector and experimental timeline of shRNA viral injection and doxycycline induction. (B) (Left) Representative images of S1PR1 expression in shS1PR1 + and shLuc + DGCs at 5 dpi. (Right) Summary plot of the S1PR1 fluorescent signal intensity. *p

    Techniques Used: shRNA, Expressing, Plasmid Preparation, Injection

    Related Articles

    Immunohistochemistry:

    Article Title: Circuit Integration Initiation of New Hippocampal Neurons in the Adult Brain
    Article Snippet: .. Immunohistochemistry was performed by blocking sections in 1% donkey serum in phosphate-buffered saline with 0.025% Triton for 1 h at room temperature and then incubating them overnight with shaking at 4°C with the following primary antibodies: GFP (rabbit polyclonal antibody, 1:1,000; Sigma-Aldrich, St. Louis, MO), Prox1 (mouse monoclonal, 1:500; Millipore, Burlington, MA), DCX (goat polyclonal antibody, 1:1,000; Santa Cruz Biotechnology, Dallas, TX), S1PR1 (rabbit polyclonal antibody, 1:500; Abcam, Cambridge, UK), phospho-S1PR1 (rabbit polyclonal antibody, 1:500, Abcam, Cambridge, UK- requires antigen retrieval protocol as previously described ( )). .. The sections were then incubated for 3 h with shaking at room temperature with the following secondary antibodies: Alexa 488-conjugated donkey anti-rabbit antibody (1:1,000; The Jackson Laboratory, Bar Harbor, ME), and Alexa 594-conjugated donkey anti-goat (1:1,000; Abcam).

    Blocking Assay:

    Article Title: Circuit Integration Initiation of New Hippocampal Neurons in the Adult Brain
    Article Snippet: .. Immunohistochemistry was performed by blocking sections in 1% donkey serum in phosphate-buffered saline with 0.025% Triton for 1 h at room temperature and then incubating them overnight with shaking at 4°C with the following primary antibodies: GFP (rabbit polyclonal antibody, 1:1,000; Sigma-Aldrich, St. Louis, MO), Prox1 (mouse monoclonal, 1:500; Millipore, Burlington, MA), DCX (goat polyclonal antibody, 1:1,000; Santa Cruz Biotechnology, Dallas, TX), S1PR1 (rabbit polyclonal antibody, 1:500; Abcam, Cambridge, UK), phospho-S1PR1 (rabbit polyclonal antibody, 1:500, Abcam, Cambridge, UK- requires antigen retrieval protocol as previously described ( )). .. The sections were then incubated for 3 h with shaking at room temperature with the following secondary antibodies: Alexa 488-conjugated donkey anti-rabbit antibody (1:1,000; The Jackson Laboratory, Bar Harbor, ME), and Alexa 594-conjugated donkey anti-goat (1:1,000; Abcam).

    Article Title: Mitochondrial biogenesis is transcriptionally repressed in lysosomal lipid storage diseases
    Article Snippet: 50 μg of sample proteins per well were subjected to Sodium dodecyl sulfate -polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (Amersham, Life Technologies). .. After blocking in 5% Milk in TBS tween, membranes were immunoblotted with the following antibodies: Sqstm1 (Abcam, ab110252), Hprt (Abcam, ab10479), Klf2 (Abcam, ab203591), Etv1 (Abcam, ab184120), Lc3b (Cell signaling, 3868), Pan Akt (Cell signaling, 4691), Phospho Akt (Cell signaling, 4060), Total Erk1/2(Cell signaling, 4695), Phospho Erk1/2 (Cell signaling, 4376), Tfam (Abcam, ab138351), P70s6k1 (Cell signaling, 2708), Phospho P70s6k1 (9234), Acc (Cell signaling, 3676), Phospho Acc (Cell signaling, 3661), Ampkα (Cell signaling, 5832), Phospho Ampkα (Cell signaling, 2535), Nrf1 (Abcam, ab175932), Atp5a, Uqcrc2, Sdhb and mtCO1 OXPHOS cocktail (Abcam, ab110413), S1pr1 (Abcam, ab125074), Cleaved PARP (Cell signaling, 5625), Cleaved Caspase-3 (Cell signaling, 9664), Laminin A/C (Cell signaling,) and Vdac1 (Abcam, ab14734). .. Band densitometric quantifications were determined using ImageJ software 1.48 v. Following normalization with Hprt, all control samples of each experiment were centered at one to ease relative comparisons with experimental samples.

    Article Title: Inhibition of miR-155-5p attenuates the valvular damage induced by rheumatic heart disease
    Article Snippet: Equal amounts of protein (20 µ g) were separated by 10% SDS-PAGE at 60 V for 30 min and 120 V for 70 min by a blotting system (Bio-Rad Laboratories, Inc.). .. The separated protein was electrotransferred to 0.22 µm PVDF membranes (EMD Millipore) at a constant 300 mA for 90 min. After electrotransfer, the membranes were blocked with 3% BSA blocking solution (Sangon Biotech Co., Ltd.) for 1 h at room temperature and then probed with the following antibodies for 12 h at 4°C: S1PR1 (1:3,000; cat. no. ab125074; Abcam), SOCS1 (1:1,000; cat. no. 3950; Cell Signaling Technology, Inc.), STAT3 (1:1,000; cat. no. ab68153; Abcam), p-STAT3 (1:1,000; cat. no. 9145; Cell Signaling Technology, Inc.) and β-tubulin (1:3,000; cat. no. 10068-1-AP; ProteinTech Group, Inc.). .. The membranes were incubated with HRP-conjugated secondary antibody (10,000; cat. no. ab6721; Abcam) for 1 h at room temperature.

    Mouse Assay:

    Article Title: Endoplasmic reticulum resident oxidase ERO1-Lalpha promotes hepatocellular carcinoma metastasis and angiogenesis through the S1PR1/STAT3/VEGF-A pathway
    Article Snippet: .. Primary antibodies were from rabbits against S1PR1, VEGF-A (Abcam, Cambridge, UK), STAT3, p-STAT3, E-cadherin, vimentin, Slug, and GAPDH (Cell Signaling Technology, Danvers, MA, USA); and from mice against ERO1α (Santa Cruz Biotechnology, Santa Cruz, CA, USA). .. ERO1α knockdown and overexpression Commercially available lentiviral-mediated ERO1α knockdown vector or the negative control (shERO1α/shNC) and lentiviral-mediated overexpressing ERO1α vector or scrambled lentiviral construct (ERO1α/Vector) were designed and produced (GenePharma Co. Ltd Shanghai, China).

    SDS Page:

    Article Title: microRNA-148a inhibits hepatocellular carcinoma cell invasion by targeting sphingosine-1-phosphate receptor 1
    Article Snippet: Western blotting Tissues or cells were solubilized in cold radioimmunoprecipitation assay lysis buffer. .. Proteins were separated with 12% SDS-PAGE and transferred onto a polyvinylidene difluoride (PVDF) membrane, which was then incubated with Tris-buffered saline and Tween 20 (Sigma-Aldrich, St. Louis, MO, USA) containing 5% milk at room temperature for 3 h. The PVDF membrane was subsequently incubated with rabbit monoclonal anti-S1PR1 (ab125074; 1:200 dilution) and -GAPDH (ab181602; 1:200 dilution) primary antibodies (Abcam, Cambridge, UK) at room temperature for 3 h. Following washing three times with phosphate-buffered saline-Tween 20, the membrane was incubated with the goat anti-rabbit secondary antibodies (Abcam) at room temperature for 40 min. Chemiluminescent detection was performed using an enhanced chemiluminescence kit (Pierce Chemical). .. The relative protein expression was analyzed by Image-Pro® Plus software 6.0 (Media Cybernetics, Inc., Silver Spring, MD, USA), and presented as the density ratio versus GAPDH.

    Incubation:

    Article Title: microRNA-148a inhibits hepatocellular carcinoma cell invasion by targeting sphingosine-1-phosphate receptor 1
    Article Snippet: Western blotting Tissues or cells were solubilized in cold radioimmunoprecipitation assay lysis buffer. .. Proteins were separated with 12% SDS-PAGE and transferred onto a polyvinylidene difluoride (PVDF) membrane, which was then incubated with Tris-buffered saline and Tween 20 (Sigma-Aldrich, St. Louis, MO, USA) containing 5% milk at room temperature for 3 h. The PVDF membrane was subsequently incubated with rabbit monoclonal anti-S1PR1 (ab125074; 1:200 dilution) and -GAPDH (ab181602; 1:200 dilution) primary antibodies (Abcam, Cambridge, UK) at room temperature for 3 h. Following washing three times with phosphate-buffered saline-Tween 20, the membrane was incubated with the goat anti-rabbit secondary antibodies (Abcam) at room temperature for 40 min. Chemiluminescent detection was performed using an enhanced chemiluminescence kit (Pierce Chemical). .. The relative protein expression was analyzed by Image-Pro® Plus software 6.0 (Media Cybernetics, Inc., Silver Spring, MD, USA), and presented as the density ratio versus GAPDH.

    Article Title: Toll-Like Receptor 4 Is Essential for the Expression of Sphingosine-1-Phosphate-Dependent Asthma-Like Disease in Mice
    Article Snippet: Samples (40 μg/lane) were separated on a 10% SDS-PAGE gel and transferred to a PVDF membrane. .. Membrane were blocked by incubation in PBS containing 0.1% v/v Tween 20 and 5% non-fat dry milk for 2 h, followed by a overnight incubation at 4°C with rabbit polyclonal anti-TLR4 (1:1,000; H-80, sc-10741; Santa Cruz) or rabbit monoclonal anti-S1P1 (1:1,000, ab125074 Abcam, UK) The filters were washed with PBS containing 0.1% v/v Tween 20 and incubated for 2 h with anti-horseradish peroxidase-conjugate secondary antibody. ..

    Electrotransfer:

    Article Title: Inhibition of miR-155-5p attenuates the valvular damage induced by rheumatic heart disease
    Article Snippet: Equal amounts of protein (20 µ g) were separated by 10% SDS-PAGE at 60 V for 30 min and 120 V for 70 min by a blotting system (Bio-Rad Laboratories, Inc.). .. The separated protein was electrotransferred to 0.22 µm PVDF membranes (EMD Millipore) at a constant 300 mA for 90 min. After electrotransfer, the membranes were blocked with 3% BSA blocking solution (Sangon Biotech Co., Ltd.) for 1 h at room temperature and then probed with the following antibodies for 12 h at 4°C: S1PR1 (1:3,000; cat. no. ab125074; Abcam), SOCS1 (1:1,000; cat. no. 3950; Cell Signaling Technology, Inc.), STAT3 (1:1,000; cat. no. ab68153; Abcam), p-STAT3 (1:1,000; cat. no. 9145; Cell Signaling Technology, Inc.) and β-tubulin (1:3,000; cat. no. 10068-1-AP; ProteinTech Group, Inc.). .. The membranes were incubated with HRP-conjugated secondary antibody (10,000; cat. no. ab6721; Abcam) for 1 h at room temperature.

    Staining:

    Article Title: Moesin Controls Clathrin-Mediated S1PR1 Internalization in T Cells
    Article Snippet: Mouse CD4+ T cells were fixed, permeabilized, and stained with the anti-S1PR1 (5 µg/ml; ab11424) or control rabbit IgG followed by Alexa Fluor 488-conjugated anti-rabbit IgG. .. The cells were also stained with the anti-S1PR1 pre-absorbed with the antigen peptide (ab39763; Abcam) at a molar ratio of 1∶20 overnight. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Abcam phospho s1pr1
    Newly Generated DGCs Highly Express <t>S1PR1</t> (A) (Top) Procedure for RNA-seq analysis of new neurons 1 day after differentiating from adult hippocampal neural stem cells. (Bottom) Expression profiling of sphingolipid signaling-related genes. Mann-Whitney U test, SPHK1 p = 0.064, S1PR1 p = 0.064, ACSL1 p = 0.064, ASAH1 p = 0.064, SMPD1 p = 0.064, DGAT1 p = 0.064, ACSS2 p = 0.355, SGPL1 p = 0.064, ALDH3A2 p = 0.04, SPNS2 p = 1.000, SGMS1 p = 0.064, S1PR2 p = 0.165, CERS2 p = 0.064, CERS5 p = 0.355, CERK p = 0.355, SPHK2 p = 0.064, SPTLC1 p = 1.000, FAR1 p = 0.064, ASAH2 p = 0.643, DEGS1 p = 0.060, RAC p = 0.165, CERS4 p = 0.064, FHL2 p =1.000, UGCG p = 0.06, ACER2 p = 0.643. (B) (Left) Region of dentate gyrus dissected. (Right) S1PR isoform mRNA detection (S1PR5 was not detected). Scale bar, 100 μm. ). (D) (Top) Retroviral GFP was delivered to the DG to label newly born DGCs. (Bottom) Representative image showing expression of S1PR1 in a DGC at 7 dpi. Scale bar, 10 μm. (E) Schematic of hippocampal/cortical dissection and lipidomics analysis. (F) Concentrations of various sphingolipid metabolites in several brain regions. DG versus Ctx: p
    Phospho S1pr1, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho s1pr1/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho s1pr1 - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

    94
    Abcam anti stat3 phospho y705 antibody ep2147y
    Reverse transcription-quantitative PCR and western blot analysis. (A) The relative mRNA expression of S1PR1, SOCS1 and <t>STAT3</t> in the four groups. (B) Western blot analysis of S1PR1, SOCS1, STAT3 and p-STAT3 in the four groups. (C) The relative protein expression of S1PR1, SOCS1, STAT3 and p-STAT3 in the four groups. (D) The ratio of phosphorylated vs. total protein for STAT3. This figure shows that expression of S1PR1 and SOCS1 was increased and expression of p-STAT3 was decreased after AAV-injection. Data are shown as the mean ± standard deviation; * P
    Anti Stat3 Phospho Y705 Antibody Ep2147y, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti stat3 phospho y705 antibody ep2147y/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti stat3 phospho y705 antibody ep2147y - by Bioz Stars, 2021-05
    94/100 stars
      Buy from Supplier

    99
    Abcam rabbit monoclonal antibodies against s1pr1
    Correlation of <t>S1PR1/STAT3</t> signaling pathway and clinical outcome. (A) A table of summary of S1PR1 and clinical outcome in different types of tumor patients. (B) Correlation of S1PR1 and SOCS3 transcriptional levels in gastric cancer patients. (C) Immuno score levels of pY-STAT3 in different stage of gastric cancer patients were compared.
    Rabbit Monoclonal Antibodies Against S1pr1, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal antibodies against s1pr1/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal antibodies against s1pr1 - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    s1pr1  (Abcam)
    93
    Abcam s1pr1
    Correlation of <t>S1PR1/STAT3</t> signaling pathway and clinical outcome. (A) A table of summary of S1PR1 and clinical outcome in different types of tumor patients. (B) Correlation of S1PR1 and SOCS3 transcriptional levels in gastric cancer patients. (C) Immuno score levels of pY-STAT3 in different stage of gastric cancer patients were compared.
    S1pr1, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s1pr1/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    s1pr1 - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    Newly Generated DGCs Highly Express S1PR1 (A) (Top) Procedure for RNA-seq analysis of new neurons 1 day after differentiating from adult hippocampal neural stem cells. (Bottom) Expression profiling of sphingolipid signaling-related genes. Mann-Whitney U test, SPHK1 p = 0.064, S1PR1 p = 0.064, ACSL1 p = 0.064, ASAH1 p = 0.064, SMPD1 p = 0.064, DGAT1 p = 0.064, ACSS2 p = 0.355, SGPL1 p = 0.064, ALDH3A2 p = 0.04, SPNS2 p = 1.000, SGMS1 p = 0.064, S1PR2 p = 0.165, CERS2 p = 0.064, CERS5 p = 0.355, CERK p = 0.355, SPHK2 p = 0.064, SPTLC1 p = 1.000, FAR1 p = 0.064, ASAH2 p = 0.643, DEGS1 p = 0.060, RAC p = 0.165, CERS4 p = 0.064, FHL2 p =1.000, UGCG p = 0.06, ACER2 p = 0.643. (B) (Left) Region of dentate gyrus dissected. (Right) S1PR isoform mRNA detection (S1PR5 was not detected). Scale bar, 100 μm. ). (D) (Top) Retroviral GFP was delivered to the DG to label newly born DGCs. (Bottom) Representative image showing expression of S1PR1 in a DGC at 7 dpi. Scale bar, 10 μm. (E) Schematic of hippocampal/cortical dissection and lipidomics analysis. (F) Concentrations of various sphingolipid metabolites in several brain regions. DG versus Ctx: p

    Journal: Cell reports

    Article Title: Circuit Integration Initiation of New Hippocampal Neurons in the Adult Brain

    doi: 10.1016/j.celrep.2019.12.084

    Figure Lengend Snippet: Newly Generated DGCs Highly Express S1PR1 (A) (Top) Procedure for RNA-seq analysis of new neurons 1 day after differentiating from adult hippocampal neural stem cells. (Bottom) Expression profiling of sphingolipid signaling-related genes. Mann-Whitney U test, SPHK1 p = 0.064, S1PR1 p = 0.064, ACSL1 p = 0.064, ASAH1 p = 0.064, SMPD1 p = 0.064, DGAT1 p = 0.064, ACSS2 p = 0.355, SGPL1 p = 0.064, ALDH3A2 p = 0.04, SPNS2 p = 1.000, SGMS1 p = 0.064, S1PR2 p = 0.165, CERS2 p = 0.064, CERS5 p = 0.355, CERK p = 0.355, SPHK2 p = 0.064, SPTLC1 p = 1.000, FAR1 p = 0.064, ASAH2 p = 0.643, DEGS1 p = 0.060, RAC p = 0.165, CERS4 p = 0.064, FHL2 p =1.000, UGCG p = 0.06, ACER2 p = 0.643. (B) (Left) Region of dentate gyrus dissected. (Right) S1PR isoform mRNA detection (S1PR5 was not detected). Scale bar, 100 μm. ). (D) (Top) Retroviral GFP was delivered to the DG to label newly born DGCs. (Bottom) Representative image showing expression of S1PR1 in a DGC at 7 dpi. Scale bar, 10 μm. (E) Schematic of hippocampal/cortical dissection and lipidomics analysis. (F) Concentrations of various sphingolipid metabolites in several brain regions. DG versus Ctx: p

    Article Snippet: Immunohistochemistry was performed by blocking sections in 1% donkey serum in phosphate-buffered saline with 0.025% Triton for 1 h at room temperature and then incubating them overnight with shaking at 4°C with the following primary antibodies: GFP (rabbit polyclonal antibody, 1:1,000; Sigma-Aldrich, St. Louis, MO), Prox1 (mouse monoclonal, 1:500; Millipore, Burlington, MA), DCX (goat polyclonal antibody, 1:1,000; Santa Cruz Biotechnology, Dallas, TX), S1PR1 (rabbit polyclonal antibody, 1:500; Abcam, Cambridge, UK), phospho-S1PR1 (rabbit polyclonal antibody, 1:500, Abcam, Cambridge, UK- requires antigen retrieval protocol as previously described ( )).

    Techniques: Generated, RNA Sequencing Assay, Expressing, MANN-WHITNEY, Dissection

    S1PR1 Knockdown Reduces Neurite Growth and Horizontal-to-Radial Repositioning (A) Description of shRNA expression vector and experimental timeline of shRNA viral injection and doxycycline induction. (B) (Left) Representative images of S1PR1 expression in shS1PR1 + and shLuc + DGCs at 5 dpi. (Right) Summary plot of the S1PR1 fluorescent signal intensity. *p

    Journal: Cell reports

    Article Title: Circuit Integration Initiation of New Hippocampal Neurons in the Adult Brain

    doi: 10.1016/j.celrep.2019.12.084

    Figure Lengend Snippet: S1PR1 Knockdown Reduces Neurite Growth and Horizontal-to-Radial Repositioning (A) Description of shRNA expression vector and experimental timeline of shRNA viral injection and doxycycline induction. (B) (Left) Representative images of S1PR1 expression in shS1PR1 + and shLuc + DGCs at 5 dpi. (Right) Summary plot of the S1PR1 fluorescent signal intensity. *p

    Article Snippet: Immunohistochemistry was performed by blocking sections in 1% donkey serum in phosphate-buffered saline with 0.025% Triton for 1 h at room temperature and then incubating them overnight with shaking at 4°C with the following primary antibodies: GFP (rabbit polyclonal antibody, 1:1,000; Sigma-Aldrich, St. Louis, MO), Prox1 (mouse monoclonal, 1:500; Millipore, Burlington, MA), DCX (goat polyclonal antibody, 1:1,000; Santa Cruz Biotechnology, Dallas, TX), S1PR1 (rabbit polyclonal antibody, 1:500; Abcam, Cambridge, UK), phospho-S1PR1 (rabbit polyclonal antibody, 1:500, Abcam, Cambridge, UK- requires antigen retrieval protocol as previously described ( )).

    Techniques: shRNA, Expressing, Plasmid Preparation, Injection

    Reverse transcription-quantitative PCR and western blot analysis. (A) The relative mRNA expression of S1PR1, SOCS1 and STAT3 in the four groups. (B) Western blot analysis of S1PR1, SOCS1, STAT3 and p-STAT3 in the four groups. (C) The relative protein expression of S1PR1, SOCS1, STAT3 and p-STAT3 in the four groups. (D) The ratio of phosphorylated vs. total protein for STAT3. This figure shows that expression of S1PR1 and SOCS1 was increased and expression of p-STAT3 was decreased after AAV-injection. Data are shown as the mean ± standard deviation; * P

    Journal: International Journal of Molecular Medicine

    Article Title: Inhibition of miR-155-5p attenuates the valvular damage induced by rheumatic heart disease

    doi: 10.3892/ijmm.2019.4420

    Figure Lengend Snippet: Reverse transcription-quantitative PCR and western blot analysis. (A) The relative mRNA expression of S1PR1, SOCS1 and STAT3 in the four groups. (B) Western blot analysis of S1PR1, SOCS1, STAT3 and p-STAT3 in the four groups. (C) The relative protein expression of S1PR1, SOCS1, STAT3 and p-STAT3 in the four groups. (D) The ratio of phosphorylated vs. total protein for STAT3. This figure shows that expression of S1PR1 and SOCS1 was increased and expression of p-STAT3 was decreased after AAV-injection. Data are shown as the mean ± standard deviation; * P

    Article Snippet: Immunohistochemistry The valve tissues stained for S1PR1 (1:80; cat. no. ab77076; Abcam), STAT3 (1:75; cat. no. ab69153; Abcam), phosphorylated (p-)STAT3 (1:70; cat. no. ab76315; Abcam), IL-6 (1:65; cat. no. ab9324; Abcam) and IL-17 (1:90; cat. no. ab214588; Abcam) were analyzed by immunohistochemistry.

    Techniques: Real-time Polymerase Chain Reaction, Western Blot, Expressing, Injection, Standard Deviation

    Immunohistochemistry analysis of S1PR1, STAT3 and p-STAT3 in valve tissues. (A) Immunohistochemistry for S1PR1, STAT3 and p-STAT3 in valve tissues; magnification, ×400; the arrows represent positive cells. (B) The IHS. (C) The positive cells percentage. This figure shows that expression of S1PR1 was increased and expression of p-STAT3 was decreased after AAV-injection. (D) The ratio of phosphorylated vs. total protein for STAT3 (ISH score). (E) The ratio of phosphorylated vs. total protein for STAT3 (positive cells percentage). Data are shown as the mean ± standard deviation; * P

    Journal: International Journal of Molecular Medicine

    Article Title: Inhibition of miR-155-5p attenuates the valvular damage induced by rheumatic heart disease

    doi: 10.3892/ijmm.2019.4420

    Figure Lengend Snippet: Immunohistochemistry analysis of S1PR1, STAT3 and p-STAT3 in valve tissues. (A) Immunohistochemistry for S1PR1, STAT3 and p-STAT3 in valve tissues; magnification, ×400; the arrows represent positive cells. (B) The IHS. (C) The positive cells percentage. This figure shows that expression of S1PR1 was increased and expression of p-STAT3 was decreased after AAV-injection. (D) The ratio of phosphorylated vs. total protein for STAT3 (ISH score). (E) The ratio of phosphorylated vs. total protein for STAT3 (positive cells percentage). Data are shown as the mean ± standard deviation; * P

    Article Snippet: Immunohistochemistry The valve tissues stained for S1PR1 (1:80; cat. no. ab77076; Abcam), STAT3 (1:75; cat. no. ab69153; Abcam), phosphorylated (p-)STAT3 (1:70; cat. no. ab76315; Abcam), IL-6 (1:65; cat. no. ab9324; Abcam) and IL-17 (1:90; cat. no. ab214588; Abcam) were analyzed by immunohistochemistry.

    Techniques: Immunohistochemistry, Expressing, Injection, In Situ Hybridization, Standard Deviation

    Correlation of S1PR1/STAT3 signaling pathway and clinical outcome. (A) A table of summary of S1PR1 and clinical outcome in different types of tumor patients. (B) Correlation of S1PR1 and SOCS3 transcriptional levels in gastric cancer patients. (C) Immuno score levels of pY-STAT3 in different stage of gastric cancer patients were compared.

    Journal: EBioMedicine

    Article Title: S1PR1 predicts patient survival and promotes chemotherapy drug resistance in gastric cancer cells through STAT3 constitutive activation

    doi: 10.1016/j.ebiom.2018.10.005

    Figure Lengend Snippet: Correlation of S1PR1/STAT3 signaling pathway and clinical outcome. (A) A table of summary of S1PR1 and clinical outcome in different types of tumor patients. (B) Correlation of S1PR1 and SOCS3 transcriptional levels in gastric cancer patients. (C) Immuno score levels of pY-STAT3 in different stage of gastric cancer patients were compared.

    Article Snippet: Rabbit monoclonal antibodies against S1PR1, Mcl-1, Bcl-xl, Survivin and GAPDH were purchased from Abcam (Cambridge, MA, USA).

    Techniques:

    Gastric cancer cell lines have different sensitivity to DDP treatment. (A) Cell viability of SGC7901 and SGC7901/DDP cells, cells were treated with fold diluted DDP. Data were expressed as mean ± SD, n = 4. (B) Cell viability of indicated gastric tumor cells, cells were treated with fold diluted DDP after transfection of S1PR1 overexpression or empty vector. Data were expressed as mean ± SD, n = 4. (C) The correlation of S1PR1 and STAT3 transcriptional levels in patients with a progressive disease outcome. (D) The correlation of S1PR1 and STAT3 transcriptional levels in patients with a complete remission/response outcome.

    Journal: EBioMedicine

    Article Title: S1PR1 predicts patient survival and promotes chemotherapy drug resistance in gastric cancer cells through STAT3 constitutive activation

    doi: 10.1016/j.ebiom.2018.10.005

    Figure Lengend Snippet: Gastric cancer cell lines have different sensitivity to DDP treatment. (A) Cell viability of SGC7901 and SGC7901/DDP cells, cells were treated with fold diluted DDP. Data were expressed as mean ± SD, n = 4. (B) Cell viability of indicated gastric tumor cells, cells were treated with fold diluted DDP after transfection of S1PR1 overexpression or empty vector. Data were expressed as mean ± SD, n = 4. (C) The correlation of S1PR1 and STAT3 transcriptional levels in patients with a progressive disease outcome. (D) The correlation of S1PR1 and STAT3 transcriptional levels in patients with a complete remission/response outcome.

    Article Snippet: Rabbit monoclonal antibodies against S1PR1, Mcl-1, Bcl-xl, Survivin and GAPDH were purchased from Abcam (Cambridge, MA, USA).

    Techniques: Transfection, Over Expression, Plasmid Preparation

    S1PR1 and pY-STAT3 were highly expressed in gastric cancer patients. (A) upper panel, Kaplan-Meier plots for overall survival of high and low transcript levels of S1PR1 in gastric cancer patients; lower panel transcript levels of S1PR1 in 4 stages of gastric cancer patients. (B) Immunohistochemistry staining of S1PR1 of gastric cancer patient tissues. Upper panel: a stage IIIb sample, lower panel: a stage IIa sample. Scale bar: 200 μM left panel, 100 μM right panel. (C) Immuno score levels of S1PR1 in different stage of gastric cancer patients were compared. (D) Correlation of S1PR1 and STAT3 transcript levels in gastric cancer patients. (E) Immunohistochemistry staining of pY-STAT3 of gastric cancer patient tissues. Upper panel stage IIIc, lower panel stage IIa Scale bar: 200 μM left panel, 100 μM right panel. (F) Correlation of S1PR1 and pY-STAT3 protein levels determined by IS in gastric cancer patients. (G) Left panel: Immunofluorescence double staining of S1PR1(green) and pY-STAT3(red) in gastric tumor, para-cancerous and normal samples, DAPI(blue) was adopted to reveal the nuclear of the cells. Scale bar: 20 μM. Right panel, quantification of patient tissue sections for percentages of overlapping red (pY-STAT3) and green (S1PR1) channels, shown as Manders colocalization coefficients M1 (p-STAT3 to S1PR1) and M2 (S1PR1 to p-STAT3), respectively.

    Journal: EBioMedicine

    Article Title: S1PR1 predicts patient survival and promotes chemotherapy drug resistance in gastric cancer cells through STAT3 constitutive activation

    doi: 10.1016/j.ebiom.2018.10.005

    Figure Lengend Snippet: S1PR1 and pY-STAT3 were highly expressed in gastric cancer patients. (A) upper panel, Kaplan-Meier plots for overall survival of high and low transcript levels of S1PR1 in gastric cancer patients; lower panel transcript levels of S1PR1 in 4 stages of gastric cancer patients. (B) Immunohistochemistry staining of S1PR1 of gastric cancer patient tissues. Upper panel: a stage IIIb sample, lower panel: a stage IIa sample. Scale bar: 200 μM left panel, 100 μM right panel. (C) Immuno score levels of S1PR1 in different stage of gastric cancer patients were compared. (D) Correlation of S1PR1 and STAT3 transcript levels in gastric cancer patients. (E) Immunohistochemistry staining of pY-STAT3 of gastric cancer patient tissues. Upper panel stage IIIc, lower panel stage IIa Scale bar: 200 μM left panel, 100 μM right panel. (F) Correlation of S1PR1 and pY-STAT3 protein levels determined by IS in gastric cancer patients. (G) Left panel: Immunofluorescence double staining of S1PR1(green) and pY-STAT3(red) in gastric tumor, para-cancerous and normal samples, DAPI(blue) was adopted to reveal the nuclear of the cells. Scale bar: 20 μM. Right panel, quantification of patient tissue sections for percentages of overlapping red (pY-STAT3) and green (S1PR1) channels, shown as Manders colocalization coefficients M1 (p-STAT3 to S1PR1) and M2 (S1PR1 to p-STAT3), respectively.

    Article Snippet: Rabbit monoclonal antibodies against S1PR1, Mcl-1, Bcl-xl, Survivin and GAPDH were purchased from Abcam (Cambridge, MA, USA).

    Techniques: Immunohistochemistry, Staining, Immunofluorescence, Double Staining

    S1PR1-STAT3 formed a positive regulatory loop in GC cells and contributed to drug resistance. (A) Expression of indicated genes of SGC7901/DDP cells after treated with different concentrations of S1P and DDP for 24 h was detected by Western blot. (B) Expression of indicated genes of SGC7901 cells transfected with S1PR1 over-expression and empty vectors for 48 h was detected by Western blot. (C) SGC7901 cells transfected with S1PR1 overexpression or empty vector cells were treated with fold diluted DDP for 24 h and the cell viability was detected by MTS method. Data were expressed as mean ± SD, n = 4. (D) Expression of indicated genes of SGC7901/DDP cells transfected with S1PR1 siRNA and mock siRNA for 48 h was determined by Western blot. (E) Cell viability of SGC7901/DDP cells, tested by MTS method. Cells were treated with fold diluted DDP for 24 h with or without S1PR1 knocking down. Data were expressed as mean ± SD, n = 4. (F) Cell apoptosis levels of SGC7091/DDP cells were determined by AnnexinV/PI double staining with FACS after indicated treatment for 24 h. The ratio of double negative population of the cells was presented with mean ± SD, n = 5. *P

    Journal: EBioMedicine

    Article Title: S1PR1 predicts patient survival and promotes chemotherapy drug resistance in gastric cancer cells through STAT3 constitutive activation

    doi: 10.1016/j.ebiom.2018.10.005

    Figure Lengend Snippet: S1PR1-STAT3 formed a positive regulatory loop in GC cells and contributed to drug resistance. (A) Expression of indicated genes of SGC7901/DDP cells after treated with different concentrations of S1P and DDP for 24 h was detected by Western blot. (B) Expression of indicated genes of SGC7901 cells transfected with S1PR1 over-expression and empty vectors for 48 h was detected by Western blot. (C) SGC7901 cells transfected with S1PR1 overexpression or empty vector cells were treated with fold diluted DDP for 24 h and the cell viability was detected by MTS method. Data were expressed as mean ± SD, n = 4. (D) Expression of indicated genes of SGC7901/DDP cells transfected with S1PR1 siRNA and mock siRNA for 48 h was determined by Western blot. (E) Cell viability of SGC7901/DDP cells, tested by MTS method. Cells were treated with fold diluted DDP for 24 h with or without S1PR1 knocking down. Data were expressed as mean ± SD, n = 4. (F) Cell apoptosis levels of SGC7091/DDP cells were determined by AnnexinV/PI double staining with FACS after indicated treatment for 24 h. The ratio of double negative population of the cells was presented with mean ± SD, n = 5. *P

    Article Snippet: Rabbit monoclonal antibodies against S1PR1, Mcl-1, Bcl-xl, Survivin and GAPDH were purchased from Abcam (Cambridge, MA, USA).

    Techniques: Expressing, Western Blot, Transfection, Over Expression, Plasmid Preparation, Double Staining, FACS

    Hyper expression of S1PR1 in gastric cancer cells identified drug resistance. (A) Transcript levels determined by QPCR and (B) protein expression levels determined by Western blot of indicated genes of SGC7901 and SGC7901/DDP gastric cancer cell lines. Data were expressed as mean ± SD, n = 5. *P

    Journal: EBioMedicine

    Article Title: S1PR1 predicts patient survival and promotes chemotherapy drug resistance in gastric cancer cells through STAT3 constitutive activation

    doi: 10.1016/j.ebiom.2018.10.005

    Figure Lengend Snippet: Hyper expression of S1PR1 in gastric cancer cells identified drug resistance. (A) Transcript levels determined by QPCR and (B) protein expression levels determined by Western blot of indicated genes of SGC7901 and SGC7901/DDP gastric cancer cell lines. Data were expressed as mean ± SD, n = 5. *P

    Article Snippet: Rabbit monoclonal antibodies against S1PR1, Mcl-1, Bcl-xl, Survivin and GAPDH were purchased from Abcam (Cambridge, MA, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

    FTY720 enhanced gastric tumor cells drug sensitivity in vivo . (A) Images of tumor bearing mice of each treatment group at the end of the experiment. (B) Body weight of mice of tumor bearing mice of each treatment group. Data were expressed as mean ± SD, n = 10. (C) H E staining and Immunohistochemistry staining of S1PR1, pY-STAT3 and Ki67 of gastric tumor tissue removed from mice. Scale bar: 10 μM.

    Journal: EBioMedicine

    Article Title: S1PR1 predicts patient survival and promotes chemotherapy drug resistance in gastric cancer cells through STAT3 constitutive activation

    doi: 10.1016/j.ebiom.2018.10.005

    Figure Lengend Snippet: FTY720 enhanced gastric tumor cells drug sensitivity in vivo . (A) Images of tumor bearing mice of each treatment group at the end of the experiment. (B) Body weight of mice of tumor bearing mice of each treatment group. Data were expressed as mean ± SD, n = 10. (C) H E staining and Immunohistochemistry staining of S1PR1, pY-STAT3 and Ki67 of gastric tumor tissue removed from mice. Scale bar: 10 μM.

    Article Snippet: Rabbit monoclonal antibodies against S1PR1, Mcl-1, Bcl-xl, Survivin and GAPDH were purchased from Abcam (Cambridge, MA, USA).

    Techniques: In Vivo, Mouse Assay, Staining, Immunohistochemistry