phospho mek1 2ser217 221  (Cell Signaling Technology Inc)

 
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    Structured Review

    Cell Signaling Technology Inc phospho mek1 2ser217 221
    Phospho Mek1 2ser217 221, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho mek1 2ser217 221/product/Cell Signaling Technology Inc
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    phospho mek1 2ser217 221 - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    Western Blot:

    Article Title: Cytosolic phospholipase A2 alpha amplifies early cyclooxygenase-2 expression, oxidative stress and MAP kinase phosphorylation after cerebral ischemia in mice
    Article Snippet: .. Immunoblotting For Western analysis, primary antibodies included COX-2 (1:1000, Cayman Chemical Co. Ann Arbor, MI), cPLA2 α (1:500), phospho-cPLA2 α (1:500), ERK1/2 and phospho-ERK1/2 (1:1000), MEK1/2 and phospho-MEK1/2 (1:1000), p38 MAPK and phospho-p38 MAPK (1:1000) (all from Cell Signalling Technology, Inc. Danvers, MA). .. Protein samples were separated by electrophoresis and transferred to PVDF membranes.

    Incubation:

    Article Title: Differences in activity and phosphorylation of MAPK enzymes in esophageal squamous cells of GERD patients with and without Barrett's esophagus
    Article Snippet: .. After separation and transfer to nitrocellulose membranes, the membranes were incubated with primary antibodies to p53 and p21 (Oncogene, San Diego, CA), cytokeratins (CK) 13 and 4 (Novocastra, Newcastle upon Tyne, UK), phospho-MEK1/2 (serines 217/221), phospho-MEK1 (threonine 286), phospho-ERK1/2, or MKP-1 (Cell Signaling Technology). .. Horseradish peroxidase secondary antibodies were used, and chemiluminescence was determined using the Super Signal West Dura detection system (Pierce).

    Variant Assay:

    Article Title: A high level of liver-specific expression of oncogenic KrasV12 drives robust liver tumorigenesis in transgenic zebrafish
    Article Snippet: .. Immunodetections were performed using the following antibodies: K-Ras (F234), which detects ubiquitously expressed K-Ras (Santa Cruz Biotechnology); K-Ras-2B (C19), which detects the C-terminus of the K-Ras-2B splice variant (Santa Cruz Biotechnology); anti-phospho-MEK1/2 (Ser217/221; Cell Signaling Technology); anti-phospho-ERK1/2 (p44/42 MAPK; Thr202/Tyr204; Cell Signaling Technology); anti-p53 (Cell Signaling Technology); anti-phospho-MDM2 (Cell Signaling Technology); anti-p21 Waf1/Cip1 (Santa Cruz Biotechnology); and anti-β-actin (Sigma). .. Signals were detected using chemiluminescence (Pierce Biotechnology) and exposure to X-ray film (Kodak).

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    • phospho mek1 2ser217 ser221  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc phospho mek1 2ser217 ser221
    PAK1 Activation is downstream of Ras Day 4 mature osteoclasts were pre-treated with a farnesyl transferase inhibitor to block Ras activation or vehicle control for seven hours. Cells were serum starved and treated with 25 ng/ml M-CSF as indicated in the presence of inhibitor or vehicle control. Western blotting for phospho-PAK1Thr423, total PAK1/3, <t>phospho-MEK1/2Ser217/221,</t> total MEK1/2, phospho-ERKThr202/Tyr204, total ERK1/2 and tubulin was then performed.
    Phospho Mek1 2ser217 Ser221, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho mek1 2ser217 ser221/product/Cell Signaling Technology Inc
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    phospho mek1 2ser217 ser221 - by Bioz Stars, 2021-01
    99/100 stars
    		  Buy from Supplier
    phospho mek1 2ser217 221  (Cell Signaling Technology Inc)
    99
    Cell Signaling Technology Inc phospho mek1 2ser217 221
    PAK1 Activation is downstream of Ras Day 4 mature osteoclasts were pre-treated with a farnesyl transferase inhibitor to block Ras activation or vehicle control for seven hours. Cells were serum starved and treated with 25 ng/ml M-CSF as indicated in the presence of inhibitor or vehicle control. Western blotting for phospho-PAK1Thr423, total PAK1/3, <t>phospho-MEK1/2Ser217/221,</t> total MEK1/2, phospho-ERKThr202/Tyr204, total ERK1/2 and tubulin was then performed.
    Phospho Mek1 2ser217 221, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho mek1 2ser217 221/product/Cell Signaling Technology Inc
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    phospho mek1 2ser217 221 - by Bioz Stars, 2021-01
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    PAK1 Activation is downstream of Ras Day 4 mature osteoclasts were pre-treated with a farnesyl transferase inhibitor to block Ras activation or vehicle control for seven hours. Cells were serum starved and treated with 25 ng/ml M-CSF as indicated in the presence of inhibitor or vehicle control. Western blotting for phospho-PAK1Thr423, total PAK1/3, phospho-MEK1/2Ser217/221, total MEK1/2, phospho-ERKThr202/Tyr204, total ERK1/2 and tubulin was then performed.

    Journal: Journal of cellular physiology

    Article Title: PAK1 IS A NOVEL MEK-INDEPENDENT RAF TARGET CONTROLLING EXPRESSION OF THE IAP SURVIVIN IN M-CSF-MEDIATED OSTEOCLAST SURVIVAL

    doi: 10.1002/jcp.21550

    Figure Lengend Snippet: PAK1 Activation is downstream of Ras Day 4 mature osteoclasts were pre-treated with a farnesyl transferase inhibitor to block Ras activation or vehicle control for seven hours. Cells were serum starved and treated with 25 ng/ml M-CSF as indicated in the presence of inhibitor or vehicle control. Western blotting for phospho-PAK1Thr423, total PAK1/3, phospho-MEK1/2Ser217/221, total MEK1/2, phospho-ERKThr202/Tyr204, total ERK1/2 and tubulin was then performed.

    Article Snippet: Western blotting was carried out using antibodies directed against total and phospho-MEK1/2Ser217/Ser221 and phospho-MEK1Ser298, total and phospho-ERK, phospho-PAK1Thr423, total PAK1/3, phospho-RafSer338, (Cell Signaling Technology, Beverly, MA) Survivin (Santa Cruz Biotechnology, Santa Cruz, CA) and tubulin (E7, Hybridoma Bank, Iowa State, IA) at a 1:1000 dilution and corresponding secondary antibodies (Cell Signaling Technology) at a 1:10,000 dilution with chemiluminescent detection using the Pierce femto reagent (Pierce, Rockford, IL).

    Techniques: Activation Assay, Blocking Assay, Western Blot

    PAK1 is a Raf Target (A) Blocking PAK1 does not affect Raf activation. Day 4 mature osteoclasts were infected with the dnPAK1 or vector control adenoviruses for 18 hours. Following infection, osteoclasts were serum starved and treated with 25 ng/ml M-CSF as indicated. Levels of phospho-RafSer338, phospho-MEKSer298, phospho-MEK1/2Ser217/221, total-MEK1/2, phospho-ERK1/2Thr202/Tyr204, total-ERK1/2 and tubulin were assayed by western blotting. (B) Blocking Raf abolishes PAK1 activation. Day 4 mature osteoclasts were infected with dnRaf or vector control adenoviruses for 18 hours. Following infection, osteoclasts were serum starved and treated with M-CSF as indicated. Western blotting for phospho-PAK1Thr423, phospho-MEK1Ser298, phospho-MEK1/2Ser217/221, total MEK1/2, phospho-ERKThr202/Tyr204, total ERK1/2 and tubulin was then performed.

    Journal: Journal of cellular physiology

    Article Title: PAK1 IS A NOVEL MEK-INDEPENDENT RAF TARGET CONTROLLING EXPRESSION OF THE IAP SURVIVIN IN M-CSF-MEDIATED OSTEOCLAST SURVIVAL

    doi: 10.1002/jcp.21550

    Figure Lengend Snippet: PAK1 is a Raf Target (A) Blocking PAK1 does not affect Raf activation. Day 4 mature osteoclasts were infected with the dnPAK1 or vector control adenoviruses for 18 hours. Following infection, osteoclasts were serum starved and treated with 25 ng/ml M-CSF as indicated. Levels of phospho-RafSer338, phospho-MEKSer298, phospho-MEK1/2Ser217/221, total-MEK1/2, phospho-ERK1/2Thr202/Tyr204, total-ERK1/2 and tubulin were assayed by western blotting. (B) Blocking Raf abolishes PAK1 activation. Day 4 mature osteoclasts were infected with dnRaf or vector control adenoviruses for 18 hours. Following infection, osteoclasts were serum starved and treated with M-CSF as indicated. Western blotting for phospho-PAK1Thr423, phospho-MEK1Ser298, phospho-MEK1/2Ser217/221, total MEK1/2, phospho-ERKThr202/Tyr204, total ERK1/2 and tubulin was then performed.

    Article Snippet: Western blotting was carried out using antibodies directed against total and phospho-MEK1/2Ser217/Ser221 and phospho-MEK1Ser298, total and phospho-ERK, phospho-PAK1Thr423, total PAK1/3, phospho-RafSer338, (Cell Signaling Technology, Beverly, MA) Survivin (Santa Cruz Biotechnology, Santa Cruz, CA) and tubulin (E7, Hybridoma Bank, Iowa State, IA) at a 1:1000 dilution and corresponding secondary antibodies (Cell Signaling Technology) at a 1:10,000 dilution with chemiluminescent detection using the Pierce femto reagent (Pierce, Rockford, IL).

    Techniques: Blocking Assay, Activation Assay, Infection, Plasmid Preparation, Western Blot

    M-CSF Mediates Survival of Mature Osteoclasts through PAK1 (A) M-CSF Activates PAK1. Day 4 mature osteoclasts were serum starved for one hour and treated with 25 ng/ml M-CSF as indicated. Levels of phospho-PAK1Thr423, total PAK1/3, phospho-MEKSer298, phospho-MEK1/2Ser217/221, total-MEK1/2, phospho-ERK1/2Thr202/Tyr204, total-ERK1/2 and tubulin were assayed by western blotting. (B and C) PAK1 suppresses osteoclast apoptosis. Day 4 mature osteoclasts were infected with the dnPAK1 or vector control adenoviruses for 18 hours. Following infection, osteoclasts were serum starved and treated with M-CSF for eight hours. Shown are (B) Percent Apoptotic Osteoclasts and (C) Total Osteoclasts. *p

    Journal: Journal of cellular physiology

    Article Title: PAK1 IS A NOVEL MEK-INDEPENDENT RAF TARGET CONTROLLING EXPRESSION OF THE IAP SURVIVIN IN M-CSF-MEDIATED OSTEOCLAST SURVIVAL

    doi: 10.1002/jcp.21550

    Figure Lengend Snippet: M-CSF Mediates Survival of Mature Osteoclasts through PAK1 (A) M-CSF Activates PAK1. Day 4 mature osteoclasts were serum starved for one hour and treated with 25 ng/ml M-CSF as indicated. Levels of phospho-PAK1Thr423, total PAK1/3, phospho-MEKSer298, phospho-MEK1/2Ser217/221, total-MEK1/2, phospho-ERK1/2Thr202/Tyr204, total-ERK1/2 and tubulin were assayed by western blotting. (B and C) PAK1 suppresses osteoclast apoptosis. Day 4 mature osteoclasts were infected with the dnPAK1 or vector control adenoviruses for 18 hours. Following infection, osteoclasts were serum starved and treated with M-CSF for eight hours. Shown are (B) Percent Apoptotic Osteoclasts and (C) Total Osteoclasts. *p

    Article Snippet: Western blotting was carried out using antibodies directed against total and phospho-MEK1/2Ser217/Ser221 and phospho-MEK1Ser298, total and phospho-ERK, phospho-PAK1Thr423, total PAK1/3, phospho-RafSer338, (Cell Signaling Technology, Beverly, MA) Survivin (Santa Cruz Biotechnology, Santa Cruz, CA) and tubulin (E7, Hybridoma Bank, Iowa State, IA) at a 1:1000 dilution and corresponding secondary antibodies (Cell Signaling Technology) at a 1:10,000 dilution with chemiluminescent detection using the Pierce femto reagent (Pierce, Rockford, IL).

    Techniques: Western Blot, Infection, Plasmid Preparation