phospho mek1 2  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88
    Name:
    Phospho MEK1 Ser217 Ser221 Antibody 13HCLCP
    Description:
    Phospho MEK1 Ser217 Ser221 Oligoclonal Antibody for Western Blot
    Catalog Number:
    710291
    Price:
    None
    Applications:
    Build Your Own Immunoassay|Cell Analysis|ELISA|ELISAs for Cell Biology & Signal Transduction|Protein Assays and Analysis|Protein Biology|Ready-To-Use Immunoassay|Western Blot Detection|Western Blotting
    Category:
    Antibodies Secondary Detection Reagents
    Buy from Supplier


    Structured Review

    Thermo Fisher phospho mek1 2
    DA-Raf-dependent inactivation of <t>MEK1/2</t> suppresses TIMP4 expression and induces a subsequent proteolytic cascade MMP14–MMP2. ( A ) Total lungs and AEC2s were collected from X/Y (pink boxes) and X – /Y (blue boxes) mice at P5, and the expression levels of Timp4 were analyzed by using real-time PCR. ( B ) Lung lysates isolated from X/Y and X – /Y mice were subjected to the gelatin zymography analysis. ( C ) Activity levels of MMP14 in the lungs of X/Y (pink bar) and X – . The values represent means ± SD. ( D ) The expression levels of Mmp2 , Mmp9 , and Mmp14 in lungs at P5 from X/Y and X – /Y mice were analyzed by real-time PCR. Values represent the amounts of mRNA relative to those in WT littermates, which are arbitrarily defined as 1. ( E ) MMP2 activity ( Top ) was analyzed by zymography using lysates from developing lungs. The amounts of TIMP4 ( Middle ) and β-Tubulin ( Bottom ) were examined by Western blot analysis at indicated postnatal ages. ( F and G ) The expression levels of Timp4 in lungs at P6 from X/Y and X – /Y mice treated with DMSO or MEKi were examined by using real-time PCR ( F ) or Western blot analysis ( G ). ( H ) MMP2 activity levels were analyzed gelatin zymography by use of lung lysates isolated from X/Y and X – /Y mice treated with DMSO or the MEKi. In A , C , D , and F the analyses were performed with three to five independent mice per genotype. ** P
    Phospho MEK1 Ser217 Ser221 Oligoclonal Antibody for Western Blot
    https://www.bioz.com/result/phospho mek1 2/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho mek1 2 - by Bioz Stars, 2020-09
    88/100 stars

    Images

    1) Product Images from "DA-Raf–dependent inhibition of the Ras-ERK signaling pathway in type 2 alveolar epithelial cells controls alveolar formation"

    Article Title: DA-Raf–dependent inhibition of the Ras-ERK signaling pathway in type 2 alveolar epithelial cells controls alveolar formation

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.1321574111

    DA-Raf-dependent inactivation of MEK1/2 suppresses TIMP4 expression and induces a subsequent proteolytic cascade MMP14–MMP2. ( A ) Total lungs and AEC2s were collected from X/Y (pink boxes) and X – /Y (blue boxes) mice at P5, and the expression levels of Timp4 were analyzed by using real-time PCR. ( B ) Lung lysates isolated from X/Y and X – /Y mice were subjected to the gelatin zymography analysis. ( C ) Activity levels of MMP14 in the lungs of X/Y (pink bar) and X – . The values represent means ± SD. ( D ) The expression levels of Mmp2 , Mmp9 , and Mmp14 in lungs at P5 from X/Y and X – /Y mice were analyzed by real-time PCR. Values represent the amounts of mRNA relative to those in WT littermates, which are arbitrarily defined as 1. ( E ) MMP2 activity ( Top ) was analyzed by zymography using lysates from developing lungs. The amounts of TIMP4 ( Middle ) and β-Tubulin ( Bottom ) were examined by Western blot analysis at indicated postnatal ages. ( F and G ) The expression levels of Timp4 in lungs at P6 from X/Y and X – /Y mice treated with DMSO or MEKi were examined by using real-time PCR ( F ) or Western blot analysis ( G ). ( H ) MMP2 activity levels were analyzed gelatin zymography by use of lung lysates isolated from X/Y and X – /Y mice treated with DMSO or the MEKi. In A , C , D , and F the analyses were performed with three to five independent mice per genotype. ** P
    Figure Legend Snippet: DA-Raf-dependent inactivation of MEK1/2 suppresses TIMP4 expression and induces a subsequent proteolytic cascade MMP14–MMP2. ( A ) Total lungs and AEC2s were collected from X/Y (pink boxes) and X – /Y (blue boxes) mice at P5, and the expression levels of Timp4 were analyzed by using real-time PCR. ( B ) Lung lysates isolated from X/Y and X – /Y mice were subjected to the gelatin zymography analysis. ( C ) Activity levels of MMP14 in the lungs of X/Y (pink bar) and X – . The values represent means ± SD. ( D ) The expression levels of Mmp2 , Mmp9 , and Mmp14 in lungs at P5 from X/Y and X – /Y mice were analyzed by real-time PCR. Values represent the amounts of mRNA relative to those in WT littermates, which are arbitrarily defined as 1. ( E ) MMP2 activity ( Top ) was analyzed by zymography using lysates from developing lungs. The amounts of TIMP4 ( Middle ) and β-Tubulin ( Bottom ) were examined by Western blot analysis at indicated postnatal ages. ( F and G ) The expression levels of Timp4 in lungs at P6 from X/Y and X – /Y mice treated with DMSO or MEKi were examined by using real-time PCR ( F ) or Western blot analysis ( G ). ( H ) MMP2 activity levels were analyzed gelatin zymography by use of lung lysates isolated from X/Y and X – /Y mice treated with DMSO or the MEKi. In A , C , D , and F the analyses were performed with three to five independent mice per genotype. ** P

    Techniques Used: Expressing, Mouse Assay, Real-time Polymerase Chain Reaction, Isolation, Zymography, Activity Assay, Western Blot

    DA-Raf–dependent inactivation of MEK1/2 in AEC2 promotes AMYF differentiation during alveolar formation. ( A ) Distribution of phosphorylated MEK1/2 (P-MEK) was analyzed in lungs of X/Y mice at P5 by immunohistochemistry. Double-immunofluorescence staining for P-MEK (green) and proSP-C ( Upper ) or α-SMA ( Lower ) was performed. Arrows indicate representative cells with high P-MEK levels. (Scale bar, 100 μm.) ( B ) The percentages of cells containing high levels of P-MEK in lungs of X/Y (pink circles) and X – . Circles and lines indicate individual mice and median values, respectively. * P
    Figure Legend Snippet: DA-Raf–dependent inactivation of MEK1/2 in AEC2 promotes AMYF differentiation during alveolar formation. ( A ) Distribution of phosphorylated MEK1/2 (P-MEK) was analyzed in lungs of X/Y mice at P5 by immunohistochemistry. Double-immunofluorescence staining for P-MEK (green) and proSP-C ( Upper ) or α-SMA ( Lower ) was performed. Arrows indicate representative cells with high P-MEK levels. (Scale bar, 100 μm.) ( B ) The percentages of cells containing high levels of P-MEK in lungs of X/Y (pink circles) and X – . Circles and lines indicate individual mice and median values, respectively. * P

    Techniques Used: Mouse Assay, Immunohistochemistry, Double Immunofluorescence Staining

    DA-Raf–deficient mice have defective alveolar formation. ( A ) The protein levels of DA-Raf, Raf family members, and phosphorylated MEK1/2 in developing lungs from WT C57BL/6 mice were analyzed by Western blotting. ( B ) Sections from lungs at the indicated postnatal days of X/Y and X – /Y mice were stained with H E. Subdivision of prealveolar saccules (pas) by alveolar septa (arrowheads) at P7 results in the generation of alveoli (a) at P14 in X/Y mice. (Scale bar, 100 μm.) ( C and D ) The number of alveoli per lung ( C ) or per unit lung volume ( D ) in X/Y (pink bars) and X – at the noted postnatal stages. The values represent means ± SD of three independent mice per genotype. ** P
    Figure Legend Snippet: DA-Raf–deficient mice have defective alveolar formation. ( A ) The protein levels of DA-Raf, Raf family members, and phosphorylated MEK1/2 in developing lungs from WT C57BL/6 mice were analyzed by Western blotting. ( B ) Sections from lungs at the indicated postnatal days of X/Y and X – /Y mice were stained with H E. Subdivision of prealveolar saccules (pas) by alveolar septa (arrowheads) at P7 results in the generation of alveoli (a) at P14 in X/Y mice. (Scale bar, 100 μm.) ( C and D ) The number of alveoli per lung ( C ) or per unit lung volume ( D ) in X/Y (pink bars) and X – at the noted postnatal stages. The values represent means ± SD of three independent mice per genotype. ** P

    Techniques Used: Mouse Assay, Western Blot, Staining

    Related Articles

    Isolation:

    Article Title: EP4 Receptor–Associated Protein in Macrophages Ameliorates Colitis and Colitis-Associated Tumorigenesis
    Article Snippet: .. For analyses of the levels of phosphorylated forms of p105, MEK and ERK, freshly isolated macrophages were fixed in 4% paraformaldehyde/PBS, permeabilized in 0.5% Triton X-100/PBS, and stained with rabbit anti–phospho-p105, anti–phospho-MEK1/2, or anti–phospho-p44/42 MAPK antibody, followed by incubation with PE-conjugated donkey anti-rabbit IgG (eBioscience). .. Flow cytometry was performed using FACSAria II and analyzed using the FlowJo software (Tree Star Inc., Ashland, OR, USA).

    Labeling:

    Article Title: DA-Raf–dependent inhibition of the Ras-ERK signaling pathway in type 2 alveolar epithelial cells controls alveolar formation
    Article Snippet: .. For double-staining of lungs with the antibodies for phospho-MEK1/2 and proSP-C or TIMP4 and proSP-C, antibodies were labeled using Zenon labeling kit (Life Technologies), according to the manufacturer’s protocol. ..

    Incubation:

    Article Title: EP4 Receptor–Associated Protein in Macrophages Ameliorates Colitis and Colitis-Associated Tumorigenesis
    Article Snippet: .. For analyses of the levels of phosphorylated forms of p105, MEK and ERK, freshly isolated macrophages were fixed in 4% paraformaldehyde/PBS, permeabilized in 0.5% Triton X-100/PBS, and stained with rabbit anti–phospho-p105, anti–phospho-MEK1/2, or anti–phospho-p44/42 MAPK antibody, followed by incubation with PE-conjugated donkey anti-rabbit IgG (eBioscience). .. Flow cytometry was performed using FACSAria II and analyzed using the FlowJo software (Tree Star Inc., Ashland, OR, USA).

    other:

    Article Title: Trastuzumab induced in vivo tissue remodelling associated in vitro with inhibition of the active forms of AKT and PTEN and RhoB induction in an ovarian carcinoma model
    Article Snippet: Antibodies The following antibodies were used: anti-Phospho-ErbB2/HER-2 (Tyr1248) provided by Upstate Ab (Euromedex, Mundolsheim, France); anti-total HER-2 (c-erbB-2/HER-2/neu Ab-12, clone CB11) and anti-tubulin-β were from NeoMarkers Ab (Interchim, Montluçon, France); anti-active MAPK pAb, rabbit (pTEpY) was from Promega (Charbonnières-les-bains, France); the phospho-AKT antibody (CR 473 Lot-6) and the total AKT antibody (Lot-6) were from Ozyme (Saint-Quentin-en-Yvelines, France); the anti-ERK (c-16) and anti-RhoB rabbit were from Santa Cruz Biotechnology (Tebu-Bio SA, Le Perray en Yvelines, France); the anti-p27Kip was from Dakocytomation (Trappes, France); the anti-total and phosphorylated form of phosphatase and tensin homologue (PTEN) were from Cell Signaling (Ozyme); the anti-phospho-MEK1/2 (Ser218/Ser222) was from Zymed Laboratories (Invitrogen, Cergy Pontoise, France) and the peroxidase-conjugated secondary mouse or rabbit antibodies were from Bio-Rad (Marnes la Coquette, France).

    Staining:

    Article Title: EP4 Receptor–Associated Protein in Macrophages Ameliorates Colitis and Colitis-Associated Tumorigenesis
    Article Snippet: .. For analyses of the levels of phosphorylated forms of p105, MEK and ERK, freshly isolated macrophages were fixed in 4% paraformaldehyde/PBS, permeabilized in 0.5% Triton X-100/PBS, and stained with rabbit anti–phospho-p105, anti–phospho-MEK1/2, or anti–phospho-p44/42 MAPK antibody, followed by incubation with PE-conjugated donkey anti-rabbit IgG (eBioscience). .. Flow cytometry was performed using FACSAria II and analyzed using the FlowJo software (Tree Star Inc., Ashland, OR, USA).

    Double Staining:

    Article Title: DA-Raf–dependent inhibition of the Ras-ERK signaling pathway in type 2 alveolar epithelial cells controls alveolar formation
    Article Snippet: .. For double-staining of lungs with the antibodies for phospho-MEK1/2 and proSP-C or TIMP4 and proSP-C, antibodies were labeled using Zenon labeling kit (Life Technologies), according to the manufacturer’s protocol. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Thermo Fisher p erk 1 2
    Effect of HG, RCS, AGE and FL-926-16 on human PDA cell proliferation, ERK 1/2 phosphorylation status and YAP activation. MIA PaCa-2 ( a ) and Panc-1 ( b ) cell proliferation after 48 h incubation with high glucose (HG, 25 mM) vs normal glucose (NG, 5 mM), with or without the carbonyl trapping agent FL-926-16 (FL, 20 mM). PDA cell (MIA PaCa-2,  c ) proliferation after 48 h incubation with the RCS glyoxal (GO) and methylglyoxal (MGO), 200 μM each, or the preformed AGE CML (100 μg/mL), with or without 20 mM FL, and relative representative bright-field images (original magnification: 100X, scale bar: 200 μm);  n  = 5 wells in duplicate per condition. Western blot analysis for phosphorylated and total ERK 1/2 in lysates ( d ) and YAP in nuclear extracts ( e ) from MIA PaCa-2 cells exposed to MGO (200 μM) or CML (100 μg/mL) for 48 h, with or without FL (20 mM), and relative band densitometry analysis from three separate experiments. Each dot in ( a ), ( b ) and ( c ), represents one well and bars represent mean ± SEM. Each dot in ( d ) and ( e ) represents a single experiment and bars represent mean ± SEM. Post hoc multiple comparison: *** P
    P Erk 1 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p erk 1 2/product/Thermo Fisher
    Average 96 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    p erk 1 2 - by Bioz Stars, 2020-09
    96/100 stars
      Buy from Supplier

    88
    Thermo Fisher phosphorylated mek1 2 pmek1 2
    Effect of HG, RCS, AGE and FL-926-16 on human PDA cell proliferation, ERK 1/2 phosphorylation status and YAP activation. MIA PaCa-2 ( a ) and Panc-1 ( b ) cell proliferation after 48 h incubation with high glucose (HG, 25 mM) vs normal glucose (NG, 5 mM), with or without the carbonyl trapping agent FL-926-16 (FL, 20 mM). PDA cell (MIA PaCa-2,  c ) proliferation after 48 h incubation with the RCS glyoxal (GO) and methylglyoxal (MGO), 200 μM each, or the preformed AGE CML (100 μg/mL), with or without 20 mM FL, and relative representative bright-field images (original magnification: 100X, scale bar: 200 μm);  n  = 5 wells in duplicate per condition. Western blot analysis for phosphorylated and total ERK 1/2 in lysates ( d ) and YAP in nuclear extracts ( e ) from MIA PaCa-2 cells exposed to MGO (200 μM) or CML (100 μg/mL) for 48 h, with or without FL (20 mM), and relative band densitometry analysis from three separate experiments. Each dot in ( a ), ( b ) and ( c ), represents one well and bars represent mean ± SEM. Each dot in ( d ) and ( e ) represents a single experiment and bars represent mean ± SEM. Post hoc multiple comparison: *** P
    Phosphorylated Mek1 2 Pmek1 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated mek1 2 pmek1 2/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated mek1 2 pmek1 2 - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    94
    Thermo Fisher phospho mek1 mek2 ser217 ser221 monoclonal antibody c 158 9
    Effect of HG, RCS, AGE and FL-926-16 on human PDA cell proliferation, ERK 1/2 phosphorylation status and YAP activation. MIA PaCa-2 ( a ) and Panc-1 ( b ) cell proliferation after 48 h incubation with high glucose (HG, 25 mM) vs normal glucose (NG, 5 mM), with or without the carbonyl trapping agent FL-926-16 (FL, 20 mM). PDA cell (MIA PaCa-2,  c ) proliferation after 48 h incubation with the RCS glyoxal (GO) and methylglyoxal (MGO), 200 μM each, or the preformed AGE CML (100 μg/mL), with or without 20 mM FL, and relative representative bright-field images (original magnification: 100X, scale bar: 200 μm);  n  = 5 wells in duplicate per condition. Western blot analysis for phosphorylated and total ERK 1/2 in lysates ( d ) and YAP in nuclear extracts ( e ) from MIA PaCa-2 cells exposed to MGO (200 μM) or CML (100 μg/mL) for 48 h, with or without FL (20 mM), and relative band densitometry analysis from three separate experiments. Each dot in ( a ), ( b ) and ( c ), represents one well and bars represent mean ± SEM. Each dot in ( d ) and ( e ) represents a single experiment and bars represent mean ± SEM. Post hoc multiple comparison: *** P
    Phospho Mek1 Mek2 Ser217 Ser221 Monoclonal Antibody C 158 9, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho mek1 mek2 ser217 ser221 monoclonal antibody c 158 9/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho mek1 mek2 ser217 ser221 monoclonal antibody c 158 9 - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Effect of HG, RCS, AGE and FL-926-16 on human PDA cell proliferation, ERK 1/2 phosphorylation status and YAP activation. MIA PaCa-2 ( a ) and Panc-1 ( b ) cell proliferation after 48 h incubation with high glucose (HG, 25 mM) vs normal glucose (NG, 5 mM), with or without the carbonyl trapping agent FL-926-16 (FL, 20 mM). PDA cell (MIA PaCa-2,  c ) proliferation after 48 h incubation with the RCS glyoxal (GO) and methylglyoxal (MGO), 200 μM each, or the preformed AGE CML (100 μg/mL), with or without 20 mM FL, and relative representative bright-field images (original magnification: 100X, scale bar: 200 μm);  n  = 5 wells in duplicate per condition. Western blot analysis for phosphorylated and total ERK 1/2 in lysates ( d ) and YAP in nuclear extracts ( e ) from MIA PaCa-2 cells exposed to MGO (200 μM) or CML (100 μg/mL) for 48 h, with or without FL (20 mM), and relative band densitometry analysis from three separate experiments. Each dot in ( a ), ( b ) and ( c ), represents one well and bars represent mean ± SEM. Each dot in ( d ) and ( e ) represents a single experiment and bars represent mean ± SEM. Post hoc multiple comparison: *** P

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Diabetes promotes invasive pancreatic cancer by increasing systemic and tumour carbonyl stress in KrasG12D/+ mice

    doi: 10.1186/s13046-020-01665-0

    Figure Lengend Snippet: Effect of HG, RCS, AGE and FL-926-16 on human PDA cell proliferation, ERK 1/2 phosphorylation status and YAP activation. MIA PaCa-2 ( a ) and Panc-1 ( b ) cell proliferation after 48 h incubation with high glucose (HG, 25 mM) vs normal glucose (NG, 5 mM), with or without the carbonyl trapping agent FL-926-16 (FL, 20 mM). PDA cell (MIA PaCa-2, c ) proliferation after 48 h incubation with the RCS glyoxal (GO) and methylglyoxal (MGO), 200 μM each, or the preformed AGE CML (100 μg/mL), with or without 20 mM FL, and relative representative bright-field images (original magnification: 100X, scale bar: 200 μm); n  = 5 wells in duplicate per condition. Western blot analysis for phosphorylated and total ERK 1/2 in lysates ( d ) and YAP in nuclear extracts ( e ) from MIA PaCa-2 cells exposed to MGO (200 μM) or CML (100 μg/mL) for 48 h, with or without FL (20 mM), and relative band densitometry analysis from three separate experiments. Each dot in ( a ), ( b ) and ( c ), represents one well and bars represent mean ± SEM. Each dot in ( d ) and ( e ) represents a single experiment and bars represent mean ± SEM. Post hoc multiple comparison: *** P

    Article Snippet: Nuclear protein levels of YAP1 and cellular protein levels of total and EGFR phosphorylated at Tyr1068 (p-EGFR), total and p-ERK 1/2 and LATS1, a key kinase of the Hippo pathway [ ], were assessed by Western blotting (see Supplementary Table S1 for antibodies in Additional file ).

    Techniques: Activation Assay, Incubation, Western Blot

    Association between AGE levels (carbonyl stress burden), ERK 1/2 phosphorilation status and YAP activation in murine and human PaC. Representative Western blots for AGEs ( a ), phosphorylated and total ERK 1/2 ( b ), CTGF ( c ) and nuclear YAP ( d ) in control (Ctr), diabetic (Diab), and Diab treated with FL-926-16 (Diab+FL) KCM mice at the time of sacrifice and relative band densitometry analysis from five mice per group. Each dot represents one case and bars represent mean ± SEM. Dual-label immunofluorescence ( d ) for AGEs (red) and YAP (green). DAPI (blue): 4′,6-diamidino-2-phenylindole. Original magnification: 250X, scale bar: 200 μm. Active (non-phosphorylated) YAP immunohistochemistry staining ( e , upper panels, original magnification: 400X, scale bar: 200 μm) in representative low and high carbonyl stress human pancreatic adenocarcinomas as assessed by their AGE level ( e , lower panels, original magnification: 250X, scale bar: 200 μm). Linear regression analysis ( f ) of the correlation between the ratio of YAP-positive nuclei to total nuclei with percentage of tumour tissue positive for AGE staining ( n  = 14). Red dots = patients with a clinical diagnosis of diabetes mellitus prior to undergoing surgery; black dots = non-diabetic subjects. Post hoc multiple comparison: *** P

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Diabetes promotes invasive pancreatic cancer by increasing systemic and tumour carbonyl stress in KrasG12D/+ mice

    doi: 10.1186/s13046-020-01665-0

    Figure Lengend Snippet: Association between AGE levels (carbonyl stress burden), ERK 1/2 phosphorilation status and YAP activation in murine and human PaC. Representative Western blots for AGEs ( a ), phosphorylated and total ERK 1/2 ( b ), CTGF ( c ) and nuclear YAP ( d ) in control (Ctr), diabetic (Diab), and Diab treated with FL-926-16 (Diab+FL) KCM mice at the time of sacrifice and relative band densitometry analysis from five mice per group. Each dot represents one case and bars represent mean ± SEM. Dual-label immunofluorescence ( d ) for AGEs (red) and YAP (green). DAPI (blue): 4′,6-diamidino-2-phenylindole. Original magnification: 250X, scale bar: 200 μm. Active (non-phosphorylated) YAP immunohistochemistry staining ( e , upper panels, original magnification: 400X, scale bar: 200 μm) in representative low and high carbonyl stress human pancreatic adenocarcinomas as assessed by their AGE level ( e , lower panels, original magnification: 250X, scale bar: 200 μm). Linear regression analysis ( f ) of the correlation between the ratio of YAP-positive nuclei to total nuclei with percentage of tumour tissue positive for AGE staining ( n  = 14). Red dots = patients with a clinical diagnosis of diabetes mellitus prior to undergoing surgery; black dots = non-diabetic subjects. Post hoc multiple comparison: *** P

    Article Snippet: Nuclear protein levels of YAP1 and cellular protein levels of total and EGFR phosphorylated at Tyr1068 (p-EGFR), total and p-ERK 1/2 and LATS1, a key kinase of the Hippo pathway [ ], were assessed by Western blotting (see Supplementary Table S1 for antibodies in Additional file ).

    Techniques: Activation Assay, Western Blot, Mouse Assay, Immunofluorescence, Immunohistochemistry, Staining