Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Paeoniflorin Inhibits LPS-Induced Activation of Splenic CD4 + T Lymphocytes and Relieves Pathological Symptoms in MRL/lpr Mice by Suppressing IRAK1 Signaling

doi: 10.1155/2022/5161890

Figure Lengend Snippet: PF suppresses the activation of the downstream NF- κ B signaling pathway in vitro. (a) Expressions of NF- κ B mRNA in cells were determined by real-time PCR. Splenocytes of MRL/MP, splenocytes of MRL/lpr, and spleen CD4 + T lymphocytes of MRL/lpr were treated with 10 μ g/mL LPS either alone or with 50 μ mol/L PF for 24 h. (b) Levels of IKK α / β , p-IKK α / β , I κ B α , p-I κ B α , NF- κ B, and p-NF- κ B protein expression in cells were determined by Western Blot. The protein bands of IKK α / β , p-IKK α / β , I κ B α , p-I κ B α , NF- κ B, and p-NF- κ B and quantitative analysis of protein expression alteration in splenocytes of MRL/MP, splenocytes of MRL/lpr, and spleen CD4 + T lymphocytes of MRL/lpr were showed. Cells in the control group were treated with vehicle (1640 with 10% FBS). The results were shown as mean ± S.D ( n = 3). ∗ and ∗∗ indicated significant differences at the levels of p < 0.05 and p < 0.01.

Article Snippet: Antibodies against p-IKK α / β (2697, monoclonal antibody), α -tubulin (2144, polyclonal antibody), and anti-mouse/rabbit IgG (7076/7074, HRP-linked Antibody) were purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Activation Assay, In Vitro, Real-time Polymerase Chain Reaction, Expressing, Western Blot

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Paeoniflorin Inhibits LPS-Induced Activation of Splenic CD4 + T Lymphocytes and Relieves Pathological Symptoms in MRL/lpr Mice by Suppressing IRAK1 Signaling

doi: 10.1155/2022/5161890

Figure Lengend Snippet: PF inhibits the activation of the downstream NF- κ B signaling pathway in vivo. (a) Expressions of NF- κ B mRNA in mice were determined by real-time PCR. (b) The protein bands of IKK α / β , p-IKK α / β , I κ B α , p-I κ B α , NF- κ B, and p-NF- κ B. (c) Quantitative analysis of protein expression alteration in spleens and kidneys of MRL/lpr were showed. MRL/MP mice treated with distilled water were used as the control group, and MRL/lpr mice treated with distilled water were used as the model group. The therapeutic concentration of PF was 50 mg/kg. The results were shown as mean ± S.D ( n = 3). ∗ and ∗∗ indicated significant differences at the levels of p < 0.05 and p < 0.01.

Article Snippet: Antibodies against p-IKK α / β (2697, monoclonal antibody), α -tubulin (2144, polyclonal antibody), and anti-mouse/rabbit IgG (7076/7074, HRP-linked Antibody) were purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Activation Assay, In Vivo, Real-time Polymerase Chain Reaction, Expressing, Concentration Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Paeoniflorin Inhibits LPS-Induced Activation of Splenic CD4 + T Lymphocytes and Relieves Pathological Symptoms in MRL/lpr Mice by Suppressing IRAK1 Signaling

doi: 10.1155/2022/5161890

Figure Lengend Snippet: Schematic illustration of the potential protective effect of PF on the progression of SLE. PF ameliorates the inflammatory activity caused by diseases through the IRAK1/IKK α / β /NF- κ B signaling pathway in the process of SLE.

Article Snippet: Antibodies against p-IKK α / β (2697, monoclonal antibody), α -tubulin (2144, polyclonal antibody), and anti-mouse/rabbit IgG (7076/7074, HRP-linked Antibody) were purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Activity Assay

Journal: BioMed Research International

Article Title: Galangin Suppresses Renal Inflammation via the Inhibition of NF- κ B, PI3K/AKT and NLRP3 in Uric Acid Treated NRK-52E Tubular Epithelial Cells

doi: 10.1155/2019/3018357

Figure Lengend Snippet: Effect of galangin on NF- κ B in UA treated NRK-52E cells. Galangin decreased the expression of the phosphorylated form of I κ B α , IKK β , and p65, and inhibited I κ B α degradation in UA treated NRK-52E cells. Each of the protein bands was normalized to band of β -actin and reported as relative intensity. Results are expressed as mean ± SEM of three independent experiments. # p < 0.05, ## p < 0.01 vs control group; ∗p < 0.05, ∗∗p < 0.01 vs UA group.

Article Snippet: The antibodies used were the following: rabbit monoclonal anti-p-I κ B α (#2859S), rabbit polyclonal anti-I κ B α (#9242S), rabbit polyclonal anti-p-p65(#3031S), rabbit monoclonal anti-p65(#8242S), rabbit monoclonal anti-p-Ikk β (#2078S), rabbit monoclonal anti-Ikk β (#8943S), rabbit monoclonal anti-NLRP3(#15101S), rabbit monoclonal anti-ASC(#67824S), rabbit polyclonal anti-caspase-1(#2225S), rabbit polyclonal anti-p-PI3K(#4228S), rabbit polyclonal anti-PI3K(#4292S), mouse monoclonal anti-p-AKT(#4051S), mouse monoclonal anti-AKT(#2920S), and rabbit polyclonal anti- β -actin(#4967S), and purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Expressing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Hwangryun-Haedok-Tang Fermented with Lactobacillus casei Suppresses Ovariectomy-Induced Bone Loss

doi: 10.1155/2012/325791

Figure Lengend Snippet: fHRT-BU inhibits RANKL-induced p38, IKK α / β , and p65 phosphorylation in BMM cells. (a) BMMs (3 × 10 5 cells/well in a 6-well plate) were pretreated with HRT-BU or fHRT-BU (10 μ g/mL) for 2 h and then stimulated with RANKL (150 ng/mL) for the indicated times. Whole cell lysates (10 μ g) were analyzed by western blot analysis with indicated antibodies. MAP kinases, IKK α / β , and p65 activation was represented by the levels of protein phosphorylation. β -actin was used as loading control. (b) BMMs were pretreated with HRT-BU or fHRT-BU (10 μ g/mL) for 2 h and then stimulated with RANKL (150 ng/mL) for 3, 6, and 12 h. Total RNA was isolated on the indicated time and mRNA expression of ICAM-1, TNF- α , Nf κ b2, and I- κ B α was analyzed by QPCR. Data represents mean ± SD of three independent experiments.

Article Snippet: Antibodies specific for phospho-ERK1/2 (Thr202/Tyr204), ERK, phospho-JNK1/2 (Thr183/Tyr185), JNK, phospho-p38 (Thr180/Tyr182), p38, phospho-IKK α / β , I- κ B α , phospho-p65 (Ser536), and p65 were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Western Blot, Activation Assay, Isolation, Expressing