phospho iκb kinase ikk α β antibody  (Cell Signaling Technology Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    IKKα Antibody
    Description:
    The NF κB Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins 1 3 Most agents that activate NF κB do so through a common pathway based on phosphorylation induced proteasome mediated degradation of IκB 3 7 The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase IKK complex whose catalysis is generally carried out by three tightly associated IKK subunits IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit 8 9 Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ Ser176 and Ser180 in IKKα which causes conformational changes resulting in kinase activation 10 13
    Catalog Number:
    2682
    Price:
    None
    Applications:
    Western Blot, Immunoprecipitation
    Category:
    Primary Antibodies
    Source:
    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the 20 amino-terminal residues of human IKKα. Antibodies are purified by protein A and peptide affinity chromatography.
    Reactivity:
    Human Mouse Rat Monkey
    Buy from Supplier


    Structured Review

    Cell Signaling Technology Inc phospho iκb kinase ikk α β antibody
    The NF κB Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins 1 3 Most agents that activate NF κB do so through a common pathway based on phosphorylation induced proteasome mediated degradation of IκB 3 7 The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase IKK complex whose catalysis is generally carried out by three tightly associated IKK subunits IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit 8 9 Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ Ser176 and Ser180 in IKKα which causes conformational changes resulting in kinase activation 10 13
    https://www.bioz.com/result/phospho iκb kinase ikk α β antibody/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho iκb kinase ikk α β antibody - by Bioz Stars, 2020-09
    99/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Insulin Receptor and IRS-1 Co-immunoprecipitation with SOCS-3, and IKK?/? Phosphorylation are Increased in Obese Zucker Rat Skeletal Muscle
    Article Snippet: .. The PVDF membranes were blocked, and then incubated with either anti-IKKα (Cat# 2682, Cell Signaling Technology, Beverly, MA), anti-IKKβ (Cat# 2370, Cell Signaling Technology), anti-IKKα/β pS 176/180 (Cat# 2694, Cell Signaling Technology), or anti-IκBα (Cat# 4812, Cell Signaling Technology) primary antibody solutions. .. Phosphorylation of IκBα pS 32/36 was determined using immunoprecipitation followed by Western blotting.

    Article Title: Interleukin (IL) 1? Induction of IL-6 Is Mediated by a Novel Phosphatidylinositol 3-Kinase-dependent AKT/I?B Kinase ? Pathway Targeting Activator Protein-1 *
    Article Snippet: .. Co-immunoprecipitation of IKK α and AKT —Lysates from control and IL-1-treated cells were incubated overnight with control rabbit IgG, anti-IKKα antibody (Cell Signaling), or AKT antibody. ..

    Blocking Assay:

    Article Title: Gastrokine 1 inhibits gastrin-induced cell proliferation
    Article Snippet: .. After blocking, the membrane was subsequently probed with antibodies against NF-κB p-p65, NF-κB p65, IKKα/β, and IκB (Cell Signaling). .. Protein bands were detected using enhanced chemiluminescence reagents (Amersham Pharmacia Biotech).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Cell Signaling Technology Inc phosphorylated iκb kinase ikk α β
    HMFO-induced nuclear factor kappa B (NF-κB) activation by the phosphorylation of inhibitor of kappa B <t>(IκB)-α</t> and IκB kinase <t>(IKK)-α/β</t> in RAW 264.7 macrophages. Cells were stimulated with HMFO (50, 100, or 200 μg/mL) or LPS (1 μg/mL). ( A ) Nuclear extracts (N) were isolated from cells stimulated with HMFO or LPS. Level of p65 in the nuclear fractions was determined by immunoblotting analysis; ( B , C ) Phosphorylated IκB-α and IKKα/β isolated from cell lysates were determined by immunoblotting analysis. β-actin was used as an internal control. Values with the different letters are significantly different ( p
    Phosphorylated Iκb Kinase Ikk α β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated iκb kinase ikk α β/product/Cell Signaling Technology Inc
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    phosphorylated iκb kinase ikk α β - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    85
    Cell Signaling Technology Inc anti phosphorylated iκb kinase ikk α β polyclonal antibodies
    HMFO-induced nuclear factor kappa B (NF-κB) activation by the phosphorylation of inhibitor of kappa B <t>(IκB)-α</t> and IκB kinase <t>(IKK)-α/β</t> in RAW 264.7 macrophages. Cells were stimulated with HMFO (50, 100, or 200 μg/mL) or LPS (1 μg/mL). ( A ) Nuclear extracts (N) were isolated from cells stimulated with HMFO or LPS. Level of p65 in the nuclear fractions was determined by immunoblotting analysis; ( B , C ) Phosphorylated IκB-α and IKKα/β isolated from cell lysates were determined by immunoblotting analysis. β-actin was used as an internal control. Values with the different letters are significantly different ( p
    Anti Phosphorylated Iκb Kinase Ikk α β Polyclonal Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phosphorylated iκb kinase ikk α β polyclonal antibodies/product/Cell Signaling Technology Inc
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti phosphorylated iκb kinase ikk α β polyclonal antibodies - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    88
    Cell Signaling Technology Inc rabbit polyclonal anti phospho iκb kinase ikk α β antibody
    HMFO-induced nuclear factor kappa B (NF-κB) activation by the phosphorylation of inhibitor of kappa B <t>(IκB)-α</t> and IκB kinase <t>(IKK)-α/β</t> in RAW 264.7 macrophages. Cells were stimulated with HMFO (50, 100, or 200 μg/mL) or LPS (1 μg/mL). ( A ) Nuclear extracts (N) were isolated from cells stimulated with HMFO or LPS. Level of p65 in the nuclear fractions was determined by immunoblotting analysis; ( B , C ) Phosphorylated IκB-α and IKKα/β isolated from cell lysates were determined by immunoblotting analysis. β-actin was used as an internal control. Values with the different letters are significantly different ( p
    Rabbit Polyclonal Anti Phospho Iκb Kinase Ikk α β Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti phospho iκb kinase ikk α β antibody/product/Cell Signaling Technology Inc
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti phospho iκb kinase ikk α β antibody - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    Image Search Results


    HMFO-induced nuclear factor kappa B (NF-κB) activation by the phosphorylation of inhibitor of kappa B (IκB)-α and IκB kinase (IKK)-α/β in RAW 264.7 macrophages. Cells were stimulated with HMFO (50, 100, or 200 μg/mL) or LPS (1 μg/mL). ( A ) Nuclear extracts (N) were isolated from cells stimulated with HMFO or LPS. Level of p65 in the nuclear fractions was determined by immunoblotting analysis; ( B , C ) Phosphorylated IκB-α and IKKα/β isolated from cell lysates were determined by immunoblotting analysis. β-actin was used as an internal control. Values with the different letters are significantly different ( p

    Journal: Nutrients

    Article Title: Immune-Enhancing Effects of a High Molecular Weight Fraction of Cynanchum wilfordii Hemsley in Macrophages and Immunosuppressed Mice

    doi: 10.3390/nu8100600

    Figure Lengend Snippet: HMFO-induced nuclear factor kappa B (NF-κB) activation by the phosphorylation of inhibitor of kappa B (IκB)-α and IκB kinase (IKK)-α/β in RAW 264.7 macrophages. Cells were stimulated with HMFO (50, 100, or 200 μg/mL) or LPS (1 μg/mL). ( A ) Nuclear extracts (N) were isolated from cells stimulated with HMFO or LPS. Level of p65 in the nuclear fractions was determined by immunoblotting analysis; ( B , C ) Phosphorylated IκB-α and IKKα/β isolated from cell lysates were determined by immunoblotting analysis. β-actin was used as an internal control. Values with the different letters are significantly different ( p

    Article Snippet: Antibodies against phosphorylated-inhibitor of kappa B (IκB)-α, phosphorylated-IκB kinase (IKK)-α/β, and phosphorylated-p38 were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

    Techniques: Activation Assay, Isolation