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rabbit anti phospho ser10 histone3  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti phospho ser10 histone3
    Rabbit Anti Phospho Ser10 Histone3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti phospho ser10 histone3/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    rabbit anti phospho ser10 histone3 - by Bioz Stars, 2025-02
    86/100 stars

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    Carl Zeiss phospho histone3 ph3
    Wing imaginal disc phenotype of salm/salr knockdowns in the prothoracic gland. (A) Wing disc size in early L3 (GFP EL3; 90 h AEL) and late L3 (GFP LL3; 120 h AEL) phtm-Gal4 UAS-GFP/+ wing discs compared with UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (salm-i/salr-i) and tub-Gal80 ts UAS-salm-RNAi; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (Gal80ts/salm-i/salr-i) wing discs from larvae grown for 2 days at 25°C, for 5 days at 17°C and for 5 days at 29°C. All values are in pixels ×10 −3 . (B) Mitotic index of early L3 (GFP EL3; 90 h AEL) and late L3 (GFP LL3; 120 h AEL) control wing discs of phtm-Gal4 UAS-GFP/+ genotype, compared with the mitotic index of UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (salm-i/salr-i) and tub-Gal80 ts /+; UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (Gal80ts/salm-i/salr-i). Data are mean±s.d. *** P <0.001 (paired t -test). (C-E′) Examples of early (C) and late L3 (E) wing discs showing the expression of phospho-Histone 3 <t>(pH3;</t> red) and DAPI (blue) in control discs ( phtm-Gal4 UAS-GFP/UAS-GFP ; C,E) and salm/salr knockdowns ( UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi ; D). (F) tub-Gal80 ts /+; UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi wing discs from larvae grown for 2 days at 25°C, for 5 days at 17°C and for 5 days at 29°C. Independent red channels are shown in C′-F′, and the outline of the wing discs is shown in blue in C′-F′. (G-K) Expression of active DcpI (red) reveals apoptotic cells in control discs ( phtm-Gal4 UAS-GFP/UAS-GFP ; G) and in salm/salr knockdown discs of UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (H) and tub-Gal80 ts /+; UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (K) genotypes. The expression of Wingless (Wg; blue) is shown in G and I, and the corresponding PG of control discs and salm/salr knockdown discs are shown in H and J.
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    Image Search Results


    Wing imaginal disc phenotype of salm/salr knockdowns in the prothoracic gland. (A) Wing disc size in early L3 (GFP EL3; 90 h AEL) and late L3 (GFP LL3; 120 h AEL) phtm-Gal4 UAS-GFP/+ wing discs compared with UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (salm-i/salr-i) and tub-Gal80 ts UAS-salm-RNAi; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (Gal80ts/salm-i/salr-i) wing discs from larvae grown for 2 days at 25°C, for 5 days at 17°C and for 5 days at 29°C. All values are in pixels ×10 −3 . (B) Mitotic index of early L3 (GFP EL3; 90 h AEL) and late L3 (GFP LL3; 120 h AEL) control wing discs of phtm-Gal4 UAS-GFP/+ genotype, compared with the mitotic index of UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (salm-i/salr-i) and tub-Gal80 ts /+; UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (Gal80ts/salm-i/salr-i). Data are mean±s.d. *** P <0.001 (paired t -test). (C-E′) Examples of early (C) and late L3 (E) wing discs showing the expression of phospho-Histone 3 (pH3; red) and DAPI (blue) in control discs ( phtm-Gal4 UAS-GFP/UAS-GFP ; C,E) and salm/salr knockdowns ( UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi ; D). (F) tub-Gal80 ts /+; UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi wing discs from larvae grown for 2 days at 25°C, for 5 days at 17°C and for 5 days at 29°C. Independent red channels are shown in C′-F′, and the outline of the wing discs is shown in blue in C′-F′. (G-K) Expression of active DcpI (red) reveals apoptotic cells in control discs ( phtm-Gal4 UAS-GFP/UAS-GFP ; G) and in salm/salr knockdown discs of UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (H) and tub-Gal80 ts /+; UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (K) genotypes. The expression of Wingless (Wg; blue) is shown in G and I, and the corresponding PG of control discs and salm/salr knockdown discs are shown in H and J.

    Journal: Development (Cambridge, England)

    Article Title: Developmental analysis of Spalt function in the Drosophila prothoracic gland

    doi: 10.1242/dev.202751

    Figure Lengend Snippet: Wing imaginal disc phenotype of salm/salr knockdowns in the prothoracic gland. (A) Wing disc size in early L3 (GFP EL3; 90 h AEL) and late L3 (GFP LL3; 120 h AEL) phtm-Gal4 UAS-GFP/+ wing discs compared with UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (salm-i/salr-i) and tub-Gal80 ts UAS-salm-RNAi; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (Gal80ts/salm-i/salr-i) wing discs from larvae grown for 2 days at 25°C, for 5 days at 17°C and for 5 days at 29°C. All values are in pixels ×10 −3 . (B) Mitotic index of early L3 (GFP EL3; 90 h AEL) and late L3 (GFP LL3; 120 h AEL) control wing discs of phtm-Gal4 UAS-GFP/+ genotype, compared with the mitotic index of UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (salm-i/salr-i) and tub-Gal80 ts /+; UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (Gal80ts/salm-i/salr-i). Data are mean±s.d. *** P <0.001 (paired t -test). (C-E′) Examples of early (C) and late L3 (E) wing discs showing the expression of phospho-Histone 3 (pH3; red) and DAPI (blue) in control discs ( phtm-Gal4 UAS-GFP/UAS-GFP ; C,E) and salm/salr knockdowns ( UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi ; D). (F) tub-Gal80 ts /+; UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi wing discs from larvae grown for 2 days at 25°C, for 5 days at 17°C and for 5 days at 29°C. Independent red channels are shown in C′-F′, and the outline of the wing discs is shown in blue in C′-F′. (G-K) Expression of active DcpI (red) reveals apoptotic cells in control discs ( phtm-Gal4 UAS-GFP/UAS-GFP ; G) and in salm/salr knockdown discs of UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (H) and tub-Gal80 ts /+; UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (K) genotypes. The expression of Wingless (Wg; blue) is shown in G and I, and the corresponding PG of control discs and salm/salr knockdown discs are shown in H and J.

    Article Snippet: We measured the wing blade area from composite z -sections (projections) of wing blade images taken using a confocal microscope (LSM750; Zeiss) and counted the number of phospho-Histone3 (pH3)-positive cells.

    Techniques: Control, Expressing, Knockdown

    Reagents and tools table

    Journal: EMBO Reports

    Article Title: Chronic spindle assembly checkpoint activation causes myelosuppression and gastrointestinal atrophy

    doi: 10.1038/s44319-024-00160-3

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: phospho-Histone3-PE , Biolegend , Cat# 650807 clone 11D8 (1:250 for FACS)*.

    Techniques: Recombinant, Sequencing, Live Cell Imaging, Cell Cycle Assay, Staining, Lysis, Software, Plasmid Preparation, Microscopy