Journal: Development (Cambridge, England)
Article Title: Developmental analysis of Spalt function in the Drosophila prothoracic gland
doi: 10.1242/dev.202751
Figure Lengend Snippet: Wing imaginal disc phenotype of salm/salr knockdowns in the prothoracic gland. (A) Wing disc size in early L3 (GFP EL3; 90 h AEL) and late L3 (GFP LL3; 120 h AEL) phtm-Gal4 UAS-GFP/+ wing discs compared with UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (salm-i/salr-i) and tub-Gal80 ts UAS-salm-RNAi; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (Gal80ts/salm-i/salr-i) wing discs from larvae grown for 2 days at 25°C, for 5 days at 17°C and for 5 days at 29°C. All values are in pixels ×10 −3 . (B) Mitotic index of early L3 (GFP EL3; 90 h AEL) and late L3 (GFP LL3; 120 h AEL) control wing discs of phtm-Gal4 UAS-GFP/+ genotype, compared with the mitotic index of UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (salm-i/salr-i) and tub-Gal80 ts /+; UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (Gal80ts/salm-i/salr-i). Data are mean±s.d. *** P <0.001 (paired t -test). (C-E′) Examples of early (C) and late L3 (E) wing discs showing the expression of phospho-Histone 3 (pH3; red) and DAPI (blue) in control discs ( phtm-Gal4 UAS-GFP/UAS-GFP ; C,E) and salm/salr knockdowns ( UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi ; D). (F) tub-Gal80 ts /+; UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi wing discs from larvae grown for 2 days at 25°C, for 5 days at 17°C and for 5 days at 29°C. Independent red channels are shown in C′-F′, and the outline of the wing discs is shown in blue in C′-F′. (G-K) Expression of active DcpI (red) reveals apoptotic cells in control discs ( phtm-Gal4 UAS-GFP/UAS-GFP ; G) and in salm/salr knockdown discs of UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (H) and tub-Gal80 ts /+; UAS-salm-RNAi/+; phtm-Gal4 UAS-GFP/UAS-salr-RNAi (K) genotypes. The expression of Wingless (Wg; blue) is shown in G and I, and the corresponding PG of control discs and salm/salr knockdown discs are shown in H and J.
Article Snippet: We measured the wing blade area from composite z -sections (projections) of wing blade images taken using a confocal microscope (LSM750; Zeiss) and counted the number of phospho-Histone3 (pH3)-positive cells.
Techniques: Control, Expressing, Knockdown