Journal: Nucleic Acids Research

Article Title: ATM, KAP1 and the Epstein–Barr virus polymerase processivity factor direct traffic at the intersection of transcription and replication

doi: 10.1093/nar/gkad823

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit anti-phospho-Histone H2AX (Ser139) antibody , Cell Signaling Technology , 9718S.

Techniques: Purification, Gel Extraction, DNA Extraction, RNA Extraction, Plasmid Preparation, SYBR Green Assay, Electroporation, In Vitro, Transfection, Recombinant

Journal: Nature Communications

Article Title: Self-triggered thermoelectric nanoheterojunction for cancer catalytic and immunotherapy

doi: 10.1038/s41467-023-40954-y

Figure Lengend Snippet: Cell viability of BST NSs, CaO 2 NPs and BST/CaO 2 NS-treated a CT26 cells and b TE1 cells by CCK8 assays. Data are presented as the mean ± s.d. ( n = 5 biologically independent cells). Statistical differences were analyzed by Student’s two-sided t -test. Representative fluorescence images and quantification of intracellular ROS by c CLSM and d FCM. Scale bar = 100 µm. Three times each experiment was repeated independently with similar results. e Representative confocal microscopy images of mitochondria-selective JC-1-stained CT26 cells after different treatments. Scale bar = 10 µm. Three times each experiment was repeated independently with similar results. f Representative confocal microscopy images of γ-H2AX-stained CT26 cells after different treatments. Scale bar = 10 µm. Three times each experiment was repeated independently with similar results. g Confocal imaging of CT26 cells stained with PI (red fluorescence) and Calcein-AM (green fluorescence) to distinguish dead cells and live cells after different treatments. Scale bar = 100 µm. Three times each experiment was repeated independently with similar results. h FCM images of CT26 cells stained with PI (red fluorescence) and Annexin V-FITC (green fluorescence) to measure cell apoptosis after treatment under different conditions.

Article Snippet: Phospho-Histone H2AX (Ser139) (D7T2V), Cell Signaling (Product # 80312), Dilution 1:200; IHC-Leica® Bond™ 1:200–1:800; Immunohistochemistry (Paraffin) 1:200–1:800; Immunofluorescence (Immunocytochemistry) 1:100–1:400; Flow Cytometry; Species Reactivity: Human, Mouse, Rat, Monkey.

Techniques: Fluorescence, Confocal Microscopy, Staining, Imaging

Journal: Nature Communications

Article Title: Self-triggered thermoelectric nanoheterojunction for cancer catalytic and immunotherapy

doi: 10.1038/s41467-023-40954-y

Figure Lengend Snippet: a , b Immunofluorescence images of the tumors and major organs (heart, liver, spleen, lung, and kidney) obtained from mice injected with BST/CaO 2 NSs. The nucleus was stained with DAPI (blue), the damaged DNA was stained with γ-H2AX foci (red), and the apoptotic cells were stained using the apoptosis marker C-CAS3 (green). Scale bars: 1000 µm for the first line and 100 µm for the second and third lines. c , d TUNEL staining of the tumors and major organs (heart, liver, spleen, lung, and kidney) obtained from mice injected with BST/CaO 2 NSs. Scale bars: 1000 µm for the first line and 100 µm for the second and third lines. Three times each experiment was repeated independently with similar results.

Article Snippet: Phospho-Histone H2AX (Ser139) (D7T2V), Cell Signaling (Product # 80312), Dilution 1:200; IHC-Leica® Bond™ 1:200–1:800; Immunohistochemistry (Paraffin) 1:200–1:800; Immunofluorescence (Immunocytochemistry) 1:100–1:400; Flow Cytometry; Species Reactivity: Human, Mouse, Rat, Monkey.

Techniques: Immunofluorescence, Injection, Staining, Marker, TUNEL Assay