Journal: Developmental cell

Article Title: Epithelial tissue confinement inhibits cell growth and leads to volume-reducing divisions

doi: 10.1016/j.devcel.2023.05.018

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-Phospho-Histone H2A.X (Ser139) , Cell Signaling Technologies , Cat# 9718; RRID:AB_2118009.

Techniques: Virus, Recombinant, Purification, Bicinchoninic Acid Protein Assay, Imaging, RNA Sequencing Assay, Clone Assay, Sequencing, Expressing, Software

Journal: iScience

Article Title: WNK1 is required during male pachynema to sustain fertility

doi: 10.1016/j.isci.2023.107616

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-phospho-histone H2A.X Ser139 , Cell Signaling Technology , Cat# 9718; RRID: AB_2118009.

Techniques: Recombinant, In Situ, Software

Journal: Communications Medicine

Article Title: Ablative radiotherapy improves survival but does not cure autochthonous mouse models of prostate and colorectal cancer

doi: 10.1038/s43856-023-00336-3

Figure Lengend Snippet: a Comparison of measured and modeled dose distribution for a 2 cm radiation field. b Representative dose distribution of a 1 cm circular pelvic radiation field mapped onto coronal (top view) and sagittal (side view) CT image of a female mouse. The reference isodose (100%) is shown in pink. Areas enclosed by the red line receive a dose >110% of the reference dose. Areas outside the dark blue line receive a dose <10% of the reference dose. c Coronal and sagittal abdominopelvic CT image of a female mouse with organs outlined as indicated. Mean organ dose in Gy is shown for a prescribed dose of 37.5 Gy. Dose distribution of a 1 cm circular pelvic radiation field is shown as a dose wash to allow visualization of organs. Dose color scheme as in ( b ). d Evaluation of focal DNA damage in a longitudinal cross-section of colorectum by γ-H2AX staining. This animal was treated with 15 Gy to a 2 cm field centered in the pelvis 30 min prior to tissue collection. Nuclear γ-H2AX foci are seen only in the portion of the colorectum that was within the radiation field. The red signal at the anus is due to autofluorescence. Composite image was generated by stitching 24 individual images taken with a 10X objective. Scale bar = 2 mm. e Evaluation of focal DNA damage by γ-H2AX IHC in prostate glands. The animal was treated with 15 Gy to a 2 cm field centered over the prostate 30 min prior to tissue collection. The lower panel shows prostate tissue from an unirradiated control. Scale bar = 100 µm.

Article Snippet: For IHC the following primary antibodies were used: Ki67 (Biocare Medical, Cat# CRM 325, RRID:AB_2721189) diluted 1:50 in TBST, Cleaved Caspase-3 (Asp175) (Cell Signaling, Cat# 9664, RRID:AB_2070042) diluted 1:800 in TBST, CD8 (Cell Signaling, Cat# 98941, RRID:AB_2756376) diluted 1:400 in TBST, FoxP3 (Cell Signaling, Cat# 12653, RRID:AB_2797979) diluted 1:200 in TBST, Phospho-Histone H2A.X (Ser139) (Cell Signaling, Cat# 9718, RRID:AB_2118009) diluted 1:600 in TBST, p21 (Abcam, Cat# ab188224, RRID:AB_2734729) diluted 1:1000 in TBST.

Techniques: Staining, Generated

Journal: Cancer cell

Article Title: Loss of SYNCRIP unleashes APOBEC-driven mutagenesis, tumor heterogeneity, and AR-targeted therapy resistance in prostate cancer

doi: 10.1016/j.ccell.2023.06.010

Figure Lengend Snippet:

Article Snippet: Rabbit anti-Phospho Histone H2A.X (Ser139) , Cell Signaling Technology , Cat #2577S; RRID: AB_2118010.

Techniques: Magnetic Beads, Recombinant, Infection, Western Blot, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Transfection, Purification, Gel Extraction, Cell Viability Assay, Single Cell Gel Electrophoresis, Software