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Cisbio Bioassays phospho erk
Phospho Erk, supplied by Cisbio Bioassays, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phospho erk/product/Cisbio Bioassays
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
phospho erk - by Bioz Stars, 2020-09
92/100 stars

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Article Title: Targeted Elimination of G Proteins and Arrestins Defines Their Specific Contributions to Both Intensity and Duration of G Protein-coupled Receptor Signaling *
Article Snippet: Quantification of phosphorylated ERK1/2 levels was performed using the phospho-ERK (Thr202 /Tyr204 ) cellular assay kit (Cisbio Bioassays).

HTRF Assay:

Article Title: G Protein-Coupled Receptor Signaling Analysis Using Homogenous Time-Resolved F?rster Resonance Energy Transfer (HTRF®) Technology
Article Snippet: .. An HTRF® based assay, Phospho-ERK (Cisbio Bioassays, Codolet, France) has been developed for measuring the level of phosphorylation of ERK1/2. .. This sandwich assay uses two antibodies; one labeled with a donor fluorophore targeting ERK1/2 and another antibody labeled with an acceptor fluorophore targeting the phosphorylated and activated kinases (pERK1/2).

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    Cisbio Bioassays htrf phospho erk kit
    Z ′-factor measurements for the <t>Phospho-ERK</t> assay . CHO cells stably expressing the muscarinic receptor 1 (M1) were used for the determination of Z ′-factor for the Phospho-ERK1/2 assay using the Phospho-ERK assay one-plate protocol. Cells were stimulated or not with 1.33 μM (EC 80 ) (A) or 4 μM (EC 100 ) (B) of carbachol for 15 min and <t>HTRF</t> signals were measured as described in Section “ Materials and Methods .” Solid lines show the means of the positive control (carbachol) and negative control (vehicle). Broken lines display three standard deviations from the mean of each data set [Adapted from the CisBio Bioassays website (see text footnote 2) with permission].
    Htrf Phospho Erk Kit, supplied by Cisbio Bioassays, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/htrf phospho erk kit/product/Cisbio Bioassays
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    htrf phospho erk kit - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

    84
    Cisbio Bioassays phospho erk assay erk1 2 phosphorylation
    K18 also reduced levels of agonist stimulated <t>ERK1/2</t> phosphorylation. pERK1/2 was detected in Flp-In-CHO cells stably expressing A 3 R (2000 cells/well) stimulated for 5 minutes with IB-MECA, with or without K18. A) Representative dose response curves for IB-MECA with K18 at the indicated concentration or DMSO control shown as mean ± SEM expressed as % 1μM PMA response. B ) pEC 50 values for individual repeats are shown as mean ± SEM . C ) Schild analysis of data represented in A/B .
    Phospho Erk Assay Erk1 2 Phosphorylation, supplied by Cisbio Bioassays, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho erk assay erk1 2 phosphorylation/product/Cisbio Bioassays
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho erk assay erk1 2 phosphorylation - by Bioz Stars, 2020-09
    84/100 stars
      Buy from Supplier

    92
    Cisbio Bioassays phospho erk
    K18 also reduced levels of agonist stimulated <t>ERK1/2</t> phosphorylation. pERK1/2 was detected in Flp-In-CHO cells stably expressing A 3 R (2000 cells/well) stimulated for 5 minutes with IB-MECA, with or without K18. A) Representative dose response curves for IB-MECA with K18 at the indicated concentration or DMSO control shown as mean ± SEM expressed as % 1μM PMA response. B ) pEC 50 values for individual repeats are shown as mean ± SEM . C ) Schild analysis of data represented in A/B .
    Phospho Erk, supplied by Cisbio Bioassays, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho erk/product/Cisbio Bioassays
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho erk - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    84
    Cisbio Bioassays homogeneous time resolved fluorescence htrf phospho erk t202 y204 cellular assay kit
    K18 also reduced levels of agonist stimulated <t>ERK1/2</t> phosphorylation. pERK1/2 was detected in Flp-In-CHO cells stably expressing A 3 R (2000 cells/well) stimulated for 5 minutes with IB-MECA, with or without K18. A) Representative dose response curves for IB-MECA with K18 at the indicated concentration or DMSO control shown as mean ± SEM expressed as % 1μM PMA response. B ) pEC 50 values for individual repeats are shown as mean ± SEM . C ) Schild analysis of data represented in A/B .
    Homogeneous Time Resolved Fluorescence Htrf Phospho Erk T202 Y204 Cellular Assay Kit, supplied by Cisbio Bioassays, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/homogeneous time resolved fluorescence htrf phospho erk t202 y204 cellular assay kit/product/Cisbio Bioassays
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    homogeneous time resolved fluorescence htrf phospho erk t202 y204 cellular assay kit - by Bioz Stars, 2020-09
    84/100 stars
      Buy from Supplier

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    Z ′-factor measurements for the Phospho-ERK assay . CHO cells stably expressing the muscarinic receptor 1 (M1) were used for the determination of Z ′-factor for the Phospho-ERK1/2 assay using the Phospho-ERK assay one-plate protocol. Cells were stimulated or not with 1.33 μM (EC 80 ) (A) or 4 μM (EC 100 ) (B) of carbachol for 15 min and HTRF signals were measured as described in Section “ Materials and Methods .” Solid lines show the means of the positive control (carbachol) and negative control (vehicle). Broken lines display three standard deviations from the mean of each data set [Adapted from the CisBio Bioassays website (see text footnote 2) with permission].

    Journal: Frontiers in Endocrinology

    Article Title: Homogeneous Time-Resolved Fluorescence-Based Assay to Monitor Extracellular Signal-Regulated Kinase Signaling in a High-Throughput Format

    doi: 10.3389/fendo.2014.00094

    Figure Lengend Snippet: Z ′-factor measurements for the Phospho-ERK assay . CHO cells stably expressing the muscarinic receptor 1 (M1) were used for the determination of Z ′-factor for the Phospho-ERK1/2 assay using the Phospho-ERK assay one-plate protocol. Cells were stimulated or not with 1.33 μM (EC 80 ) (A) or 4 μM (EC 100 ) (B) of carbachol for 15 min and HTRF signals were measured as described in Section “ Materials and Methods .” Solid lines show the means of the positive control (carbachol) and negative control (vehicle). Broken lines display three standard deviations from the mean of each data set [Adapted from the CisBio Bioassays website (see text footnote 2) with permission].

    Article Snippet: Cell lines, plasmids, and reagents We used the HTRF® Phospho-ERK kit (Catalog number 64ERKPEG) recently launched by Cisbio Bioassays.

    Techniques: Stable Transfection, Expressing, Positive Control, Negative Control

    EGF-promoted Phospho-ERK1/2 activation detected by the Phospho-ERK assay . (A) Total ERK levels quantified in various cell lines. (B) Kinetics of EGF-induced ERK1/2 activation in HEK293 cells endogenously expressing EGFR upon their stimulation with 100 nM of EGF. (C,D) The effect of cell density on the phosphorylation of ERK1/2 (C) versus total ERK1/2 (D) upon stimulation with increasing concentrations of EGF as indicated. EGF-induced Phospho-ERK1/2 in various cell lines endogenously expressing EGFR: NIH-3T3 (E) , SKOV3 (F) , and HEK293 (G) cells (10 5 cells/well) stimulated for 5 min with increasing concentrations of EGF before HTRF measurements were performed using the one-plate protocol. The data are mean ± SEM of three independent experiments performed in duplicate.

    Journal: Frontiers in Endocrinology

    Article Title: Homogeneous Time-Resolved Fluorescence-Based Assay to Monitor Extracellular Signal-Regulated Kinase Signaling in a High-Throughput Format

    doi: 10.3389/fendo.2014.00094

    Figure Lengend Snippet: EGF-promoted Phospho-ERK1/2 activation detected by the Phospho-ERK assay . (A) Total ERK levels quantified in various cell lines. (B) Kinetics of EGF-induced ERK1/2 activation in HEK293 cells endogenously expressing EGFR upon their stimulation with 100 nM of EGF. (C,D) The effect of cell density on the phosphorylation of ERK1/2 (C) versus total ERK1/2 (D) upon stimulation with increasing concentrations of EGF as indicated. EGF-induced Phospho-ERK1/2 in various cell lines endogenously expressing EGFR: NIH-3T3 (E) , SKOV3 (F) , and HEK293 (G) cells (10 5 cells/well) stimulated for 5 min with increasing concentrations of EGF before HTRF measurements were performed using the one-plate protocol. The data are mean ± SEM of three independent experiments performed in duplicate.

    Article Snippet: Cell lines, plasmids, and reagents We used the HTRF® Phospho-ERK kit (Catalog number 64ERKPEG) recently launched by Cisbio Bioassays.

    Techniques: Activation Assay, Expressing

    Comparison of the Phospho-ERK and western blot assays, and effect of cell density . A431 cells were used for the detection of the phosphorylation of ERK1/2 upon cell stimulation with 100 nM of EGF for 5 min (A) as well as the corresponding total ERK levels using the one-plate protocol (B) . S/N represents the signal-to-noise ratio through the different cell densities. For this, serial dilutions of whole cells or cell lysate were dispensed as indicated and analyzed side-by-side using the HTRF assay [top of (A,B) ] and western blot [bottom of (A,B) ] as described in Section “ Materials and Methods .” (C) The detection limit of the total ERK levels using the two methods was represented by plotting the total HTRF signals (HTRF) with the enhanced chemiluminescence (ECL) signal obtained by densitometry from the western blot (WB) normalized to the signal obtained with 60,000 cells/well as 100%. The data are mean ± SEM of three independent experiments performed in duplicate.

    Journal: Frontiers in Endocrinology

    Article Title: Homogeneous Time-Resolved Fluorescence-Based Assay to Monitor Extracellular Signal-Regulated Kinase Signaling in a High-Throughput Format

    doi: 10.3389/fendo.2014.00094

    Figure Lengend Snippet: Comparison of the Phospho-ERK and western blot assays, and effect of cell density . A431 cells were used for the detection of the phosphorylation of ERK1/2 upon cell stimulation with 100 nM of EGF for 5 min (A) as well as the corresponding total ERK levels using the one-plate protocol (B) . S/N represents the signal-to-noise ratio through the different cell densities. For this, serial dilutions of whole cells or cell lysate were dispensed as indicated and analyzed side-by-side using the HTRF assay [top of (A,B) ] and western blot [bottom of (A,B) ] as described in Section “ Materials and Methods .” (C) The detection limit of the total ERK levels using the two methods was represented by plotting the total HTRF signals (HTRF) with the enhanced chemiluminescence (ECL) signal obtained by densitometry from the western blot (WB) normalized to the signal obtained with 60,000 cells/well as 100%. The data are mean ± SEM of three independent experiments performed in duplicate.

    Article Snippet: Cell lines, plasmids, and reagents We used the HTRF® Phospho-ERK kit (Catalog number 64ERKPEG) recently launched by Cisbio Bioassays.

    Techniques: Western Blot, Cell Stimulation, HTRF Assay

    Inhibition of ERK1/2 pathway assessed by the Phospho-ERK assay . CHO cells stably expressing MOP3R (A) and HEK293 cells transiently expressing V2R (B) , PAR1 (C) , or NMUR2 (D) were used for the detection of ERK1/2 phosphorylation upon cell stimulation with 2 nM of DAMGO (A) , 100 nM of AVP (B) , 1 U/ml of thrombin (C) , or 30 nM of NmU-25 (D) for 5 min, using 10 5 cells/well and the two-plate protocol. To assess the inhibition, cells were first pre-treated either with CTOP (A) for 15 min or overnight with 200 ng/ml of PTX (B–D) before agonist stimulation and HTRF measurements. The data are mean ± SEM of three independent experiments performed in duplicate.

    Journal: Frontiers in Endocrinology

    Article Title: Homogeneous Time-Resolved Fluorescence-Based Assay to Monitor Extracellular Signal-Regulated Kinase Signaling in a High-Throughput Format

    doi: 10.3389/fendo.2014.00094

    Figure Lengend Snippet: Inhibition of ERK1/2 pathway assessed by the Phospho-ERK assay . CHO cells stably expressing MOP3R (A) and HEK293 cells transiently expressing V2R (B) , PAR1 (C) , or NMUR2 (D) were used for the detection of ERK1/2 phosphorylation upon cell stimulation with 2 nM of DAMGO (A) , 100 nM of AVP (B) , 1 U/ml of thrombin (C) , or 30 nM of NmU-25 (D) for 5 min, using 10 5 cells/well and the two-plate protocol. To assess the inhibition, cells were first pre-treated either with CTOP (A) for 15 min or overnight with 200 ng/ml of PTX (B–D) before agonist stimulation and HTRF measurements. The data are mean ± SEM of three independent experiments performed in duplicate.

    Article Snippet: Cell lines, plasmids, and reagents We used the HTRF® Phospho-ERK kit (Catalog number 64ERKPEG) recently launched by Cisbio Bioassays.

    Techniques: Inhibition, Stable Transfection, Expressing, Cell Stimulation

    Principle of the Phospho-ERK assay . (A) Principle of HTRF ® -based ERK1/2 assay that consists of three experimental steps: activation, cell lysis, and HTRF detection to quantify the total ERK1/2 as well as the phosphorylation of ERK1/2 mediated by the major cell surface receptors. This straightforward assay has been developed with two different protocols: (B) the one-plate protocol where all the assay steps are performed in the total volume of 20 μl using one 384-well small volume plate, and (C) the two-plate protocol in which the stimulation and lysis steps are performed in the total volume of 50 μl using the initial 96-well plate containing the cells, then the cell lysate is transferred into a 384-well small volume plate for HTRF detection after addition of HTRF conjugated-antibodies as described in Section “ Materials and Methods ” (Adapted from the CisBio Bioassays website 2 with permission).

    Journal: Frontiers in Endocrinology

    Article Title: Homogeneous Time-Resolved Fluorescence-Based Assay to Monitor Extracellular Signal-Regulated Kinase Signaling in a High-Throughput Format

    doi: 10.3389/fendo.2014.00094

    Figure Lengend Snippet: Principle of the Phospho-ERK assay . (A) Principle of HTRF ® -based ERK1/2 assay that consists of three experimental steps: activation, cell lysis, and HTRF detection to quantify the total ERK1/2 as well as the phosphorylation of ERK1/2 mediated by the major cell surface receptors. This straightforward assay has been developed with two different protocols: (B) the one-plate protocol where all the assay steps are performed in the total volume of 20 μl using one 384-well small volume plate, and (C) the two-plate protocol in which the stimulation and lysis steps are performed in the total volume of 50 μl using the initial 96-well plate containing the cells, then the cell lysate is transferred into a 384-well small volume plate for HTRF detection after addition of HTRF conjugated-antibodies as described in Section “ Materials and Methods ” (Adapted from the CisBio Bioassays website 2 with permission).

    Article Snippet: Cell lines, plasmids, and reagents We used the HTRF® Phospho-ERK kit (Catalog number 64ERKPEG) recently launched by Cisbio Bioassays.

    Techniques: Activation Assay, Lysis

    K18 also reduced levels of agonist stimulated ERK1/2 phosphorylation. pERK1/2 was detected in Flp-In-CHO cells stably expressing A 3 R (2000 cells/well) stimulated for 5 minutes with IB-MECA, with or without K18. A) Representative dose response curves for IB-MECA with K18 at the indicated concentration or DMSO control shown as mean ± SEM expressed as % 1μM PMA response. B ) pEC 50 values for individual repeats are shown as mean ± SEM . C ) Schild analysis of data represented in A/B .

    Journal: bioRxiv

    Article Title: Pharmacological Characterisation of Novel Adenosine Receptor A3R Antagonists

    doi: 10.1101/693796

    Figure Lengend Snippet: K18 also reduced levels of agonist stimulated ERK1/2 phosphorylation. pERK1/2 was detected in Flp-In-CHO cells stably expressing A 3 R (2000 cells/well) stimulated for 5 minutes with IB-MECA, with or without K18. A) Representative dose response curves for IB-MECA with K18 at the indicated concentration or DMSO control shown as mean ± SEM expressed as % 1μM PMA response. B ) pEC 50 values for individual repeats are shown as mean ± SEM . C ) Schild analysis of data represented in A/B .

    Article Snippet: Phospho-ERK assay ERK1/2 phosphorylation was measured using the homogeneous time resolved fluorescence (HTRF)® Phospho-ERK (T202/Y204) Cellular Assay Kit (Cisbio Bioassays, Codolet, France) two-plate format in accordance with the manufacturer’s instructions.

    Techniques: Stable Transfection, Expressing, Concentration Assay