phospho egfr y845  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho egfr y845
    Phospho Egfr Y845, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti phospho egfr y845  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho egfr y845
    Anti Phospho Egfr Y845, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phospho egfr y845  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho egfr y845
    Effect of genistein on expression and phosphorylation of <t>EGFR</t> during experimental CCl 4 -induced subacute liver damage. An increase in total protein and phosphorylation from livers with CCl 4 -induced subacute liver damage was observed. Genistein significantly increased total protein and pY845 and pY1068 EGFR. The analysis was determined by Western blot a semiquantitative analysis of the expression levels of p‑EGFR <t>(Y845,</t> Y992 and Y1068), β-actin was used as a loading control. Bars show the mean values ± standard deviations of the band density normalized to the total protein. n = 8, *p < 0.05 as compared to control group; #p < 0.05 as compared to liver damage group, respectively using an ANOVA and Tukey’s test
    Phospho Egfr Y845, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "EGF-receptor phosphorylation and downstream signaling are activated by genistein during subacute liver damage"

    Article Title: EGF-receptor phosphorylation and downstream signaling are activated by genistein during subacute liver damage

    Journal: Journal of Molecular Histology

    doi: 10.1007/s10735-023-10127-8

    Effect of genistein on expression and phosphorylation of EGFR during experimental CCl 4 -induced subacute liver damage. An increase in total protein and phosphorylation from livers with CCl 4 -induced subacute liver damage was observed. Genistein significantly increased total protein and pY845 and pY1068 EGFR. The analysis was determined by Western blot a semiquantitative analysis of the expression levels of p‑EGFR (Y845, Y992 and Y1068), β-actin was used as a loading control. Bars show the mean values ± standard deviations of the band density normalized to the total protein. n = 8, *p < 0.05 as compared to control group; #p < 0.05 as compared to liver damage group, respectively using an ANOVA and Tukey’s test
    Figure Legend Snippet: Effect of genistein on expression and phosphorylation of EGFR during experimental CCl 4 -induced subacute liver damage. An increase in total protein and phosphorylation from livers with CCl 4 -induced subacute liver damage was observed. Genistein significantly increased total protein and pY845 and pY1068 EGFR. The analysis was determined by Western blot a semiquantitative analysis of the expression levels of p‑EGFR (Y845, Y992 and Y1068), β-actin was used as a loading control. Bars show the mean values ± standard deviations of the band density normalized to the total protein. n = 8, *p < 0.05 as compared to control group; #p < 0.05 as compared to liver damage group, respectively using an ANOVA and Tukey’s test

    Techniques Used: Expressing, Western Blot

    phospho egfr y845  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho egfr y845
    Phospho Egfr Y845, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phospho y845 egfr  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho y845 egfr
    Phospho Y845 Egfr, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phospho egfr y845  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho egfr y845
    Phospho Egfr Y845, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phospho egfr y845  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho egfr y845
    Phospho Egfr Y845, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p egfr y845  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p egfr y845
    P Egfr Y845, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p egfr y845  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p egfr y845
    The effect of 4‐PP on the UVB‐induced phosphorylation of c‐Src and <t>EGFR</t> in HaCaT cells. (A) 4‐PP inhibits the UVB‐induced phosphorylation, EGFR (Y1068) and EGFR (Y1045) in HaCaT cells, but had no effect on phosphorylation of c‐Src. (B) 4‐PP inhibits the UVB‐induced phosphorylation of EGFR <t>(Y845)</t> in HaCaT cells. Cells were pretreated with 4‐PP for 1 h, irradiated with UVB, and then harvested after 15 min. Phosphorylation and expression levels were detected by western blot assay with specific antibodies. The data represent the mean ± SD of three independent experiments. # p < 0.05 between the control group and the group exposed to UVB alone; ** p < 0.01 and *** p < 0.001 between groups irradiated with UVB and 4‐PP and the group exposed to UVB alone. (C) 4‐PP directly binds to c‐Src in HaCaT cells. For the DARTS assay, cells lysate was treated with 4‐PP at the indicated concentrations for 1 h and were digested with pronase and assessed via western blot assay. The data represent the mean ± SD of three independent experiments. ** p < 0.01 between groups treated with pronase and 4‐PP and the group treated with pronase only
    P Egfr Y845, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "4‐phenylpyridine suppresses UVB ‐induced skin inflammation by targeting c‐Src in vitro and in vivo"

    Article Title: 4‐phenylpyridine suppresses UVB ‐induced skin inflammation by targeting c‐Src in vitro and in vivo

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.17422

    The effect of 4‐PP on the UVB‐induced phosphorylation of c‐Src and EGFR in HaCaT cells. (A) 4‐PP inhibits the UVB‐induced phosphorylation, EGFR (Y1068) and EGFR (Y1045) in HaCaT cells, but had no effect on phosphorylation of c‐Src. (B) 4‐PP inhibits the UVB‐induced phosphorylation of EGFR (Y845) in HaCaT cells. Cells were pretreated with 4‐PP for 1 h, irradiated with UVB, and then harvested after 15 min. Phosphorylation and expression levels were detected by western blot assay with specific antibodies. The data represent the mean ± SD of three independent experiments. # p < 0.05 between the control group and the group exposed to UVB alone; ** p < 0.01 and *** p < 0.001 between groups irradiated with UVB and 4‐PP and the group exposed to UVB alone. (C) 4‐PP directly binds to c‐Src in HaCaT cells. For the DARTS assay, cells lysate was treated with 4‐PP at the indicated concentrations for 1 h and were digested with pronase and assessed via western blot assay. The data represent the mean ± SD of three independent experiments. ** p < 0.01 between groups treated with pronase and 4‐PP and the group treated with pronase only
    Figure Legend Snippet: The effect of 4‐PP on the UVB‐induced phosphorylation of c‐Src and EGFR in HaCaT cells. (A) 4‐PP inhibits the UVB‐induced phosphorylation, EGFR (Y1068) and EGFR (Y1045) in HaCaT cells, but had no effect on phosphorylation of c‐Src. (B) 4‐PP inhibits the UVB‐induced phosphorylation of EGFR (Y845) in HaCaT cells. Cells were pretreated with 4‐PP for 1 h, irradiated with UVB, and then harvested after 15 min. Phosphorylation and expression levels were detected by western blot assay with specific antibodies. The data represent the mean ± SD of three independent experiments. # p < 0.05 between the control group and the group exposed to UVB alone; ** p < 0.01 and *** p < 0.001 between groups irradiated with UVB and 4‐PP and the group exposed to UVB alone. (C) 4‐PP directly binds to c‐Src in HaCaT cells. For the DARTS assay, cells lysate was treated with 4‐PP at the indicated concentrations for 1 h and were digested with pronase and assessed via western blot assay. The data represent the mean ± SD of three independent experiments. ** p < 0.01 between groups treated with pronase and 4‐PP and the group treated with pronase only

    Techniques Used: Irradiation, Expressing, Western Blot

    The effect of 4‐PP on UVB‐induced epidermal thickness, phosphorylation of EGFR (Y845) and COX‐2 expression in ICR mice. (A) 4‐PP inhibits UVB‐induced increasing mouse epidermal thickness. Haematoxylin and eosin‐stained images of UVB‐irradiated mouse skin. Images are representative of results from 5 tissue samples. (B) 4‐PP inhibits UVB‐induced phosphorylation of EGFR (Y845) in ICR mice. (C) and (D) Western blot assay and immunofluorescence assay show that 4‐PP inhibits UVB‐induced COX‐2 expression in ICR mice. Phosphorylation and expression levels of mice dorsal skin were detected by western blot assay. The data represent the mean ± SD of three independent experiments. # p < 0.05 between the control group and the group exposed to UVB alone; * p < 0.05, ** p < 0.01 and *** p < 0.001 between groups irradiated with UVB and 4‐PP and the group exposed to UVB alone
    Figure Legend Snippet: The effect of 4‐PP on UVB‐induced epidermal thickness, phosphorylation of EGFR (Y845) and COX‐2 expression in ICR mice. (A) 4‐PP inhibits UVB‐induced increasing mouse epidermal thickness. Haematoxylin and eosin‐stained images of UVB‐irradiated mouse skin. Images are representative of results from 5 tissue samples. (B) 4‐PP inhibits UVB‐induced phosphorylation of EGFR (Y845) in ICR mice. (C) and (D) Western blot assay and immunofluorescence assay show that 4‐PP inhibits UVB‐induced COX‐2 expression in ICR mice. Phosphorylation and expression levels of mice dorsal skin were detected by western blot assay. The data represent the mean ± SD of three independent experiments. # p < 0.05 between the control group and the group exposed to UVB alone; * p < 0.05, ** p < 0.01 and *** p < 0.001 between groups irradiated with UVB and 4‐PP and the group exposed to UVB alone

    Techniques Used: Expressing, Staining, Irradiation, Western Blot, Immunofluorescence

    anti phospho egfr y845  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho egfr y845
    Anti Phospho Egfr Y845, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p egfr y845  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p egfr y845
    BTC is both necessary and sufficient to stimulate <t>EGFR</t> signaling (A) NIH3T3 cells expressing either empty vector or the V5-tagged BTC ORF were analyzed for the indicated proteins by immunoblotting. ACTB was used as a loading control. (B) HSAEC1-KT cells expressing either empty vector or the V5-tagged BTC ORF were analyzed for the indicated proteins by immunoblotting. ACTB was used as a loading control. (C) PC9 cells expressing either a non-silencing shRNA or BTC shRNA were analyzed for indicated proteins by immunoblotting. ACTB was used as a loading control. (D) HCC2935 cells expressing either a non-silencing shRNA or BTC shRNA were analyzed for indicated proteins by immunoblotting. ACTB was used as a loading control. (E) Proteome Profiler Mouse Phospho-RTK Array membranes were incubated with lysates from NIH3T3 cells expressing either empty vector or V5-tagged BTC ORF. Array membranes incubated with lysates from vector or V5-tagged BTC ORF is shown. (F) Quantification of <t>p</t> <t>-EGFR,</t> p -ERBB2 and p -IGF1R for data shown in (E). (G) Proteome Profiler Human Phospho-RTK Array membranes showing relative RTK phosphorylation in PC9 cells expressing NS or BTC specific shRNAs is shown. (H) Quantification of p -EGFR, p -ERBB2 and p -ERBB3 for data shown in panel (G). Data are shown as the mean ±SEM See also and .
    P Egfr Y845, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Betacellulin promotes tumor development and EGFR mutant lung cancer growth by stimulating the EGFR pathway and suppressing apoptosis"

    Article Title: Betacellulin promotes tumor development and EGFR mutant lung cancer growth by stimulating the EGFR pathway and suppressing apoptosis

    Journal: iScience

    doi: 10.1016/j.isci.2022.104211

    BTC is both necessary and sufficient to stimulate EGFR signaling (A) NIH3T3 cells expressing either empty vector or the V5-tagged BTC ORF were analyzed for the indicated proteins by immunoblotting. ACTB was used as a loading control. (B) HSAEC1-KT cells expressing either empty vector or the V5-tagged BTC ORF were analyzed for the indicated proteins by immunoblotting. ACTB was used as a loading control. (C) PC9 cells expressing either a non-silencing shRNA or BTC shRNA were analyzed for indicated proteins by immunoblotting. ACTB was used as a loading control. (D) HCC2935 cells expressing either a non-silencing shRNA or BTC shRNA were analyzed for indicated proteins by immunoblotting. ACTB was used as a loading control. (E) Proteome Profiler Mouse Phospho-RTK Array membranes were incubated with lysates from NIH3T3 cells expressing either empty vector or V5-tagged BTC ORF. Array membranes incubated with lysates from vector or V5-tagged BTC ORF is shown. (F) Quantification of p -EGFR, p -ERBB2 and p -IGF1R for data shown in (E). (G) Proteome Profiler Human Phospho-RTK Array membranes showing relative RTK phosphorylation in PC9 cells expressing NS or BTC specific shRNAs is shown. (H) Quantification of p -EGFR, p -ERBB2 and p -ERBB3 for data shown in panel (G). Data are shown as the mean ±SEM See also and .
    Figure Legend Snippet: BTC is both necessary and sufficient to stimulate EGFR signaling (A) NIH3T3 cells expressing either empty vector or the V5-tagged BTC ORF were analyzed for the indicated proteins by immunoblotting. ACTB was used as a loading control. (B) HSAEC1-KT cells expressing either empty vector or the V5-tagged BTC ORF were analyzed for the indicated proteins by immunoblotting. ACTB was used as a loading control. (C) PC9 cells expressing either a non-silencing shRNA or BTC shRNA were analyzed for indicated proteins by immunoblotting. ACTB was used as a loading control. (D) HCC2935 cells expressing either a non-silencing shRNA or BTC shRNA were analyzed for indicated proteins by immunoblotting. ACTB was used as a loading control. (E) Proteome Profiler Mouse Phospho-RTK Array membranes were incubated with lysates from NIH3T3 cells expressing either empty vector or V5-tagged BTC ORF. Array membranes incubated with lysates from vector or V5-tagged BTC ORF is shown. (F) Quantification of p -EGFR, p -ERBB2 and p -IGF1R for data shown in (E). (G) Proteome Profiler Human Phospho-RTK Array membranes showing relative RTK phosphorylation in PC9 cells expressing NS or BTC specific shRNAs is shown. (H) Quantification of p -EGFR, p -ERBB2 and p -ERBB3 for data shown in panel (G). Data are shown as the mean ±SEM See also and .

    Techniques Used: Expressing, Plasmid Preparation, Western Blot, shRNA, Incubation


    Figure Legend Snippet:

    Techniques Used: Neutralization, Microarray, Mutagenesis, Recombinant, Transfection, Staining, Plasmid Preparation, shRNA, Software

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