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Modulation of PKA/CREB and ERK signaling by BF in B16F10 cells. ( a ) Representative immunoblots showing the expression of PKA, p-PKA, CREB and p-CREB. ( b ) Representative immunoblots showing the expression of ERK1/2, and p-ERK1/2. ( c – e ) Quantification of p-PKA/PKA ( c ), p-CREB/CREB ( d ), and p-ERK1/2/ERK1/2 ( e ) ratios normalized to β-actin. Data are presented as mean ± SD ( n = 3). * p < 0.05 vs. α-MSH group.

Journal: Molecules

Article Title: Baeckea frutescens Suppresses Melanogenesis via Modulation of PKA/CREB and ERK/MAPK Pathways: Insights from Cellular, Zebrafish, and In Silico Analyses

doi: 10.3390/molecules31101685

Figure Lengend Snippet: Modulation of PKA/CREB and ERK signaling by BF in B16F10 cells. ( a ) Representative immunoblots showing the expression of PKA, p-PKA, CREB and p-CREB. ( b ) Representative immunoblots showing the expression of ERK1/2, and p-ERK1/2. ( c – e ) Quantification of p-PKA/PKA ( c ), p-CREB/CREB ( d ), and p-ERK1/2/ERK1/2 ( e ) ratios normalized to β-actin. Data are presented as mean ± SD ( n = 3). * p < 0.05 vs. α-MSH group.

Article Snippet: Primary antibodies against TYR, DCT, MITF, CREB, p-CREB (S133), PKA C-α, p-PKA C-α, AKT, p-AKT (S473), ERK1/2, p-ERK1/2 (T202/Y204), JNK 1/2/3, p-JNK 1/2/3 (T183/T183/T221), GSK-3β, p-GSK-3β (S9), β-catenin, and β-actin were purchased from ABclonal (Woburn, MA, USA).

Techniques: Western Blot, Expressing