phospho camkii  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho camkii
    JOSD2 aggravates ISO-induced myocardial injury via activation of CaMKIIδ. WT mice were infected with AAV9 carrying JOSD2 cDNA (AAV9-JOSD2) or an empty vector (AAV9-NC) through tail vein. Four weeks post-injection, mice were infused with ISO. During the ISO-infused period, KN-93 was administered at 5 mg/kg/day. A Representative M-mode echocardiographic images from mice in each group. B Representative images from harvested heart tissues (scale bar: 2.5 mm). C–I Representative images and quantification of heart tissues stained with WGA (C–D), H&E (E), Sirius Red (F–G) and Masson (H–I) in sections of hearts (scale bar: 100 μm). J The content of ANP in plasma. K mRNA levels of hypertrophy-associated genes and fibrosis-associated genes in mouse heart tissues. Data was normalized to Actb. L Western blot analysis of hypertrophy-associated β-MyHC, ANP and fibrosis-associated COL-1, TGF-β1 in heart tissues. GAPDH was used as the loading control. M Western blot analysis of p-CaMKIIδ, CaMKIIδ in heart tissues. GAPDH was used as the loading control. All quantitative data is presented as Mean ± SEM; n = 6; ns = not significant; * = p < 0.05
    Phospho Camkii, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho camkii - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "JOSD2 mediates isoprenaline-induced heart failure by deubiquitinating CaMKIIδ in cardiomyocytes"

    Article Title: JOSD2 mediates isoprenaline-induced heart failure by deubiquitinating CaMKIIδ in cardiomyocytes

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-023-05037-7

    JOSD2 aggravates ISO-induced myocardial injury via activation of CaMKIIδ. WT mice were infected with AAV9 carrying JOSD2 cDNA (AAV9-JOSD2) or an empty vector (AAV9-NC) through tail vein. Four weeks post-injection, mice were infused with ISO. During the ISO-infused period, KN-93 was administered at 5 mg/kg/day. A Representative M-mode echocardiographic images from mice in each group. B Representative images from harvested heart tissues (scale bar: 2.5 mm). C–I Representative images and quantification of heart tissues stained with WGA (C–D), H&E (E), Sirius Red (F–G) and Masson (H–I) in sections of hearts (scale bar: 100 μm). J The content of ANP in plasma. K mRNA levels of hypertrophy-associated genes and fibrosis-associated genes in mouse heart tissues. Data was normalized to Actb. L Western blot analysis of hypertrophy-associated β-MyHC, ANP and fibrosis-associated COL-1, TGF-β1 in heart tissues. GAPDH was used as the loading control. M Western blot analysis of p-CaMKIIδ, CaMKIIδ in heart tissues. GAPDH was used as the loading control. All quantitative data is presented as Mean ± SEM; n = 6; ns = not significant; * = p < 0.05
    Figure Legend Snippet: JOSD2 aggravates ISO-induced myocardial injury via activation of CaMKIIδ. WT mice were infected with AAV9 carrying JOSD2 cDNA (AAV9-JOSD2) or an empty vector (AAV9-NC) through tail vein. Four weeks post-injection, mice were infused with ISO. During the ISO-infused period, KN-93 was administered at 5 mg/kg/day. A Representative M-mode echocardiographic images from mice in each group. B Representative images from harvested heart tissues (scale bar: 2.5 mm). C–I Representative images and quantification of heart tissues stained with WGA (C–D), H&E (E), Sirius Red (F–G) and Masson (H–I) in sections of hearts (scale bar: 100 μm). J The content of ANP in plasma. K mRNA levels of hypertrophy-associated genes and fibrosis-associated genes in mouse heart tissues. Data was normalized to Actb. L Western blot analysis of hypertrophy-associated β-MyHC, ANP and fibrosis-associated COL-1, TGF-β1 in heart tissues. GAPDH was used as the loading control. M Western blot analysis of p-CaMKIIδ, CaMKIIδ in heart tissues. GAPDH was used as the loading control. All quantitative data is presented as Mean ± SEM; n = 6; ns = not significant; * = p < 0.05

    Techniques Used: Activation Assay, Infection, Plasmid Preparation, Injection, Staining, Western Blot

    JOSD2 interacts CaMKIIδ directly and promotes CaMKIIδ activation. A-B MS/MS spectrum of the peptide showing FTDEYQLFEELGK and ESTESSNTTIEDEDVK from CaMKIIδ. Single-letter abbreviations: K, Lys; V, Val; I, Ile; G, Gly; Q, Gln; L, Leu; N, Asn; E, Glu; T, Thr; S, Ser; F, Phe; D, Asp; Y, Tyr. m/z, mass/charge ratio. C The complex of JOSD2 and CaMKIIδ in NRVMs were detected by Coimmunoprecipitation. NRVMs were transfected with Flag-tagged JOSD2. Lysates from cells were immunoprecipitated with an anti-Flag antibody. CaMKIIδ levels were detected by immunoblotting. IgG was used as a control for IP. D In HEK-293 T cells which express a few endogenous CaMKII, Flag-tagged JOSD2 and GFP-tagged CaMKIIδ were transfected. Lysates from cells were immunoprecipitated with anti-Flag antibody. GFP levels were detected by immunoblotting. IgG was used as a control for IP. E Representative western blot for CaMKIIδ and p-CaMKIIδ in NRVMs with gradient levels of JOSD2. F–G JOSD2 was silenced or overexpressed in NRVMs. Representative western blotting for CaMKIIδ and p-CaMKIIδ in NRVMs. GAPDH was used as a loading control. (H-I) Representative western blotting for CaMKIIδ and p-CaMKIIδ in heart tissues from ISO- and MI-induced mice. J–K Confocal images (J)and representative recordings (K) of SR Ca2+ determined by the Fluo-4 AM fluorescence signal in cardiomyocytes transfected with si-JOSD2 or si-NC before ISO stimulation for 24 h. n = 3 cells in each group. [Scale bar, 50 μm]. L–M Confocal images (L) and representative recordings (M) of SR Ca2+ determined by the Fluo-4 AM fluorescence signal in cardiomyocytes transfected with Flag-JOSD2 or empty vector (EV) before ISO stimulation for 24 h. n = 6 cells in each group. [Scale bar, 50 μm]
    Figure Legend Snippet: JOSD2 interacts CaMKIIδ directly and promotes CaMKIIδ activation. A-B MS/MS spectrum of the peptide showing FTDEYQLFEELGK and ESTESSNTTIEDEDVK from CaMKIIδ. Single-letter abbreviations: K, Lys; V, Val; I, Ile; G, Gly; Q, Gln; L, Leu; N, Asn; E, Glu; T, Thr; S, Ser; F, Phe; D, Asp; Y, Tyr. m/z, mass/charge ratio. C The complex of JOSD2 and CaMKIIδ in NRVMs were detected by Coimmunoprecipitation. NRVMs were transfected with Flag-tagged JOSD2. Lysates from cells were immunoprecipitated with an anti-Flag antibody. CaMKIIδ levels were detected by immunoblotting. IgG was used as a control for IP. D In HEK-293 T cells which express a few endogenous CaMKII, Flag-tagged JOSD2 and GFP-tagged CaMKIIδ were transfected. Lysates from cells were immunoprecipitated with anti-Flag antibody. GFP levels were detected by immunoblotting. IgG was used as a control for IP. E Representative western blot for CaMKIIδ and p-CaMKIIδ in NRVMs with gradient levels of JOSD2. F–G JOSD2 was silenced or overexpressed in NRVMs. Representative western blotting for CaMKIIδ and p-CaMKIIδ in NRVMs. GAPDH was used as a loading control. (H-I) Representative western blotting for CaMKIIδ and p-CaMKIIδ in heart tissues from ISO- and MI-induced mice. J–K Confocal images (J)and representative recordings (K) of SR Ca2+ determined by the Fluo-4 AM fluorescence signal in cardiomyocytes transfected with si-JOSD2 or si-NC before ISO stimulation for 24 h. n = 3 cells in each group. [Scale bar, 50 μm]. L–M Confocal images (L) and representative recordings (M) of SR Ca2+ determined by the Fluo-4 AM fluorescence signal in cardiomyocytes transfected with Flag-JOSD2 or empty vector (EV) before ISO stimulation for 24 h. n = 6 cells in each group. [Scale bar, 50 μm]

    Techniques Used: Activation Assay, Tandem Mass Spectroscopy, Transfection, Immunoprecipitation, Western Blot, Fluorescence, Plasmid Preparation

    phospho camkii  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc phospho camkii
    JOSD2 aggravates ISO-induced myocardial injury via activation of CaMKIIδ. WT mice were infected with AAV9 carrying JOSD2 cDNA (AAV9-JOSD2) or an empty vector (AAV9-NC) through tail vein. Four weeks post-injection, mice were infused with ISO. During the ISO-infused period, KN-93 was administered at 5 mg/kg/day. A Representative M-mode echocardiographic images from mice in each group. B Representative images from harvested heart tissues (scale bar: 2.5 mm). C–I Representative images and quantification of heart tissues stained with WGA (C–D), H&E (E), Sirius Red (F–G) and Masson (H–I) in sections of hearts (scale bar: 100 μm). J The content of ANP in plasma. K mRNA levels of hypertrophy-associated genes and fibrosis-associated genes in mouse heart tissues. Data was normalized to Actb. L Western blot analysis of hypertrophy-associated β-MyHC, ANP and fibrosis-associated COL-1, TGF-β1 in heart tissues. GAPDH was used as the loading control. M Western blot analysis of p-CaMKIIδ, CaMKIIδ in heart tissues. GAPDH was used as the loading control. All quantitative data is presented as Mean ± SEM; n = 6; ns = not significant; * = p < 0.05
    Phospho Camkii, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    phospho camkii - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "JOSD2 mediates isoprenaline-induced heart failure by deubiquitinating CaMKIIδ in cardiomyocytes"

    Article Title: JOSD2 mediates isoprenaline-induced heart failure by deubiquitinating CaMKIIδ in cardiomyocytes

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-023-05037-7

    JOSD2 aggravates ISO-induced myocardial injury via activation of CaMKIIδ. WT mice were infected with AAV9 carrying JOSD2 cDNA (AAV9-JOSD2) or an empty vector (AAV9-NC) through tail vein. Four weeks post-injection, mice were infused with ISO. During the ISO-infused period, KN-93 was administered at 5 mg/kg/day. A Representative M-mode echocardiographic images from mice in each group. B Representative images from harvested heart tissues (scale bar: 2.5 mm). C–I Representative images and quantification of heart tissues stained with WGA (C–D), H&E (E), Sirius Red (F–G) and Masson (H–I) in sections of hearts (scale bar: 100 μm). J The content of ANP in plasma. K mRNA levels of hypertrophy-associated genes and fibrosis-associated genes in mouse heart tissues. Data was normalized to Actb. L Western blot analysis of hypertrophy-associated β-MyHC, ANP and fibrosis-associated COL-1, TGF-β1 in heart tissues. GAPDH was used as the loading control. M Western blot analysis of p-CaMKIIδ, CaMKIIδ in heart tissues. GAPDH was used as the loading control. All quantitative data is presented as Mean ± SEM; n = 6; ns = not significant; * = p < 0.05
    Figure Legend Snippet: JOSD2 aggravates ISO-induced myocardial injury via activation of CaMKIIδ. WT mice were infected with AAV9 carrying JOSD2 cDNA (AAV9-JOSD2) or an empty vector (AAV9-NC) through tail vein. Four weeks post-injection, mice were infused with ISO. During the ISO-infused period, KN-93 was administered at 5 mg/kg/day. A Representative M-mode echocardiographic images from mice in each group. B Representative images from harvested heart tissues (scale bar: 2.5 mm). C–I Representative images and quantification of heart tissues stained with WGA (C–D), H&E (E), Sirius Red (F–G) and Masson (H–I) in sections of hearts (scale bar: 100 μm). J The content of ANP in plasma. K mRNA levels of hypertrophy-associated genes and fibrosis-associated genes in mouse heart tissues. Data was normalized to Actb. L Western blot analysis of hypertrophy-associated β-MyHC, ANP and fibrosis-associated COL-1, TGF-β1 in heart tissues. GAPDH was used as the loading control. M Western blot analysis of p-CaMKIIδ, CaMKIIδ in heart tissues. GAPDH was used as the loading control. All quantitative data is presented as Mean ± SEM; n = 6; ns = not significant; * = p < 0.05

    Techniques Used: Activation Assay, Infection, Plasmid Preparation, Injection, Staining, Western Blot

    JOSD2 interacts CaMKIIδ directly and promotes CaMKIIδ activation. A-B MS/MS spectrum of the peptide showing FTDEYQLFEELGK and ESTESSNTTIEDEDVK from CaMKIIδ. Single-letter abbreviations: K, Lys; V, Val; I, Ile; G, Gly; Q, Gln; L, Leu; N, Asn; E, Glu; T, Thr; S, Ser; F, Phe; D, Asp; Y, Tyr. m/z, mass/charge ratio. C The complex of JOSD2 and CaMKIIδ in NRVMs were detected by Coimmunoprecipitation. NRVMs were transfected with Flag-tagged JOSD2. Lysates from cells were immunoprecipitated with an anti-Flag antibody. CaMKIIδ levels were detected by immunoblotting. IgG was used as a control for IP. D In HEK-293 T cells which express a few endogenous CaMKII, Flag-tagged JOSD2 and GFP-tagged CaMKIIδ were transfected. Lysates from cells were immunoprecipitated with anti-Flag antibody. GFP levels were detected by immunoblotting. IgG was used as a control for IP. E Representative western blot for CaMKIIδ and p-CaMKIIδ in NRVMs with gradient levels of JOSD2. F–G JOSD2 was silenced or overexpressed in NRVMs. Representative western blotting for CaMKIIδ and p-CaMKIIδ in NRVMs. GAPDH was used as a loading control. (H-I) Representative western blotting for CaMKIIδ and p-CaMKIIδ in heart tissues from ISO- and MI-induced mice. J–K Confocal images (J)and representative recordings (K) of SR Ca2+ determined by the Fluo-4 AM fluorescence signal in cardiomyocytes transfected with si-JOSD2 or si-NC before ISO stimulation for 24 h. n = 3 cells in each group. [Scale bar, 50 μm]. L–M Confocal images (L) and representative recordings (M) of SR Ca2+ determined by the Fluo-4 AM fluorescence signal in cardiomyocytes transfected with Flag-JOSD2 or empty vector (EV) before ISO stimulation for 24 h. n = 6 cells in each group. [Scale bar, 50 μm]
    Figure Legend Snippet: JOSD2 interacts CaMKIIδ directly and promotes CaMKIIδ activation. A-B MS/MS spectrum of the peptide showing FTDEYQLFEELGK and ESTESSNTTIEDEDVK from CaMKIIδ. Single-letter abbreviations: K, Lys; V, Val; I, Ile; G, Gly; Q, Gln; L, Leu; N, Asn; E, Glu; T, Thr; S, Ser; F, Phe; D, Asp; Y, Tyr. m/z, mass/charge ratio. C The complex of JOSD2 and CaMKIIδ in NRVMs were detected by Coimmunoprecipitation. NRVMs were transfected with Flag-tagged JOSD2. Lysates from cells were immunoprecipitated with an anti-Flag antibody. CaMKIIδ levels were detected by immunoblotting. IgG was used as a control for IP. D In HEK-293 T cells which express a few endogenous CaMKII, Flag-tagged JOSD2 and GFP-tagged CaMKIIδ were transfected. Lysates from cells were immunoprecipitated with anti-Flag antibody. GFP levels were detected by immunoblotting. IgG was used as a control for IP. E Representative western blot for CaMKIIδ and p-CaMKIIδ in NRVMs with gradient levels of JOSD2. F–G JOSD2 was silenced or overexpressed in NRVMs. Representative western blotting for CaMKIIδ and p-CaMKIIδ in NRVMs. GAPDH was used as a loading control. (H-I) Representative western blotting for CaMKIIδ and p-CaMKIIδ in heart tissues from ISO- and MI-induced mice. J–K Confocal images (J)and representative recordings (K) of SR Ca2+ determined by the Fluo-4 AM fluorescence signal in cardiomyocytes transfected with si-JOSD2 or si-NC before ISO stimulation for 24 h. n = 3 cells in each group. [Scale bar, 50 μm]. L–M Confocal images (L) and representative recordings (M) of SR Ca2+ determined by the Fluo-4 AM fluorescence signal in cardiomyocytes transfected with Flag-JOSD2 or empty vector (EV) before ISO stimulation for 24 h. n = 6 cells in each group. [Scale bar, 50 μm]

    Techniques Used: Activation Assay, Tandem Mass Spectroscopy, Transfection, Immunoprecipitation, Western Blot, Fluorescence, Plasmid Preparation

    rabbit monoclonal anti phospho camkii thr286  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit monoclonal anti phospho camkii thr286
    Rabbit Monoclonal Anti Phospho Camkii Thr286, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho camkii thr286/product/Cell Signaling Technology Inc
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    rabbit monoclonal anti phospho camkii thr286  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal anti phospho camkii thr286
    Rabbit Monoclonal Anti Phospho Camkii Thr286, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho camkii thr286/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    phospho camkii t286  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho camkii t286
    Lack of Ng increases <t>CaMKII</t> signaling and periostin expression in the heart. A. Heart lysates from Ng−/− mice exhibit significant increases in Ca2+-CaM signaling. B. Ng−/− mice exhibit increased trends for CaM expression in the heart lysate. C. Phospho-CaMKII expression <t>(T286)</t> is significantly increased in the heart of Ng−/− mice. D-E. There are increased trends of protein expression of pan-CaMKII and CaN. F. Lack of Ng does not affect RyR expression in the heart. G. Significantly increased periostin expression is observed in the heart of Ng−/− mice (n = 4 per genotype). H. Ng siRNA knockdown (n = 6) in HL-1 cell line exhibits altered CaMKII activity compared to those of mock (n = 3) and lipofectamine (n = 3) controls. I. Phospho-CaMKII expression (T286) was significantly increased by Ng siRNA in HL-1, while there was no change in CaMKIIδ expression. *p < 0.05.
    Phospho Camkii T286, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho camkii t286/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho camkii t286 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Neurogranin regulates calcium-dependent cardiac hypertrophy"

    Article Title: Neurogranin regulates calcium-dependent cardiac hypertrophy

    Journal: Experimental and molecular pathology

    doi: 10.1016/j.yexmp.2022.104815

    Lack of Ng increases CaMKII signaling and periostin expression in the heart. A. Heart lysates from Ng−/− mice exhibit significant increases in Ca2+-CaM signaling. B. Ng−/− mice exhibit increased trends for CaM expression in the heart lysate. C. Phospho-CaMKII expression (T286) is significantly increased in the heart of Ng−/− mice. D-E. There are increased trends of protein expression of pan-CaMKII and CaN. F. Lack of Ng does not affect RyR expression in the heart. G. Significantly increased periostin expression is observed in the heart of Ng−/− mice (n = 4 per genotype). H. Ng siRNA knockdown (n = 6) in HL-1 cell line exhibits altered CaMKII activity compared to those of mock (n = 3) and lipofectamine (n = 3) controls. I. Phospho-CaMKII expression (T286) was significantly increased by Ng siRNA in HL-1, while there was no change in CaMKIIδ expression. *p < 0.05.
    Figure Legend Snippet: Lack of Ng increases CaMKII signaling and periostin expression in the heart. A. Heart lysates from Ng−/− mice exhibit significant increases in Ca2+-CaM signaling. B. Ng−/− mice exhibit increased trends for CaM expression in the heart lysate. C. Phospho-CaMKII expression (T286) is significantly increased in the heart of Ng−/− mice. D-E. There are increased trends of protein expression of pan-CaMKII and CaN. F. Lack of Ng does not affect RyR expression in the heart. G. Significantly increased periostin expression is observed in the heart of Ng−/− mice (n = 4 per genotype). H. Ng siRNA knockdown (n = 6) in HL-1 cell line exhibits altered CaMKII activity compared to those of mock (n = 3) and lipofectamine (n = 3) controls. I. Phospho-CaMKII expression (T286) was significantly increased by Ng siRNA in HL-1, while there was no change in CaMKIIδ expression. *p < 0.05.

    Techniques Used: Expressing, Activity Assay

    phospho camkii t286  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc phospho camkii t286
    Lack of Ng increases <t>CaMKII</t> signaling and periostin expression in the heart. A. Heart lysates from Ng−/− mice exhibit significant increases in Ca2+-CaM signaling. B. Ng−/− mice exhibit increased trends for CaM expression in the heart lysate. C. Phospho-CaMKII expression <t>(T286)</t> is significantly increased in the heart of Ng−/− mice. D-E. There are increased trends of protein expression of pan-CaMKII and CaN. F. Lack of Ng does not affect RyR expression in the heart. G. Significantly increased periostin expression is observed in the heart of Ng−/− mice (n = 4 per genotype). H. Ng siRNA knockdown (n = 6) in HL-1 cell line exhibits altered CaMKII activity compared to those of mock (n = 3) and lipofectamine (n = 3) controls. I. Phospho-CaMKII expression (T286) was significantly increased by Ng siRNA in HL-1, while there was no change in CaMKIIδ expression. *p < 0.05.
    Phospho Camkii T286, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho camkii t286/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho camkii t286 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Neurogranin regulates calcium-dependent cardiac hypertrophy"

    Article Title: Neurogranin regulates calcium-dependent cardiac hypertrophy

    Journal: Experimental and molecular pathology

    doi: 10.1016/j.yexmp.2022.104815

    Lack of Ng increases CaMKII signaling and periostin expression in the heart. A. Heart lysates from Ng−/− mice exhibit significant increases in Ca2+-CaM signaling. B. Ng−/− mice exhibit increased trends for CaM expression in the heart lysate. C. Phospho-CaMKII expression (T286) is significantly increased in the heart of Ng−/− mice. D-E. There are increased trends of protein expression of pan-CaMKII and CaN. F. Lack of Ng does not affect RyR expression in the heart. G. Significantly increased periostin expression is observed in the heart of Ng−/− mice (n = 4 per genotype). H. Ng siRNA knockdown (n = 6) in HL-1 cell line exhibits altered CaMKII activity compared to those of mock (n = 3) and lipofectamine (n = 3) controls. I. Phospho-CaMKII expression (T286) was significantly increased by Ng siRNA in HL-1, while there was no change in CaMKIIδ expression. *p < 0.05.
    Figure Legend Snippet: Lack of Ng increases CaMKII signaling and periostin expression in the heart. A. Heart lysates from Ng−/− mice exhibit significant increases in Ca2+-CaM signaling. B. Ng−/− mice exhibit increased trends for CaM expression in the heart lysate. C. Phospho-CaMKII expression (T286) is significantly increased in the heart of Ng−/− mice. D-E. There are increased trends of protein expression of pan-CaMKII and CaN. F. Lack of Ng does not affect RyR expression in the heart. G. Significantly increased periostin expression is observed in the heart of Ng−/− mice (n = 4 per genotype). H. Ng siRNA knockdown (n = 6) in HL-1 cell line exhibits altered CaMKII activity compared to those of mock (n = 3) and lipofectamine (n = 3) controls. I. Phospho-CaMKII expression (T286) was significantly increased by Ng siRNA in HL-1, while there was no change in CaMKIIδ expression. *p < 0.05.

    Techniques Used: Expressing, Activity Assay

    phospho camkii thr286  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc phospho camkii thr286
    Phospho Camkii Thr286, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho camkii thr286/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho camkii thr286 - by Bioz Stars, 2024-07
    86/100 stars

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    phospho camkii thr286  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc phospho camkii thr286
    Phospho Camkii Thr286, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho camkii thr286/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho camkii thr286 - by Bioz Stars, 2024-07
    86/100 stars

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    rabbit monoclonal anti phospho camkii thr286  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal anti phospho camkii thr286

    Rabbit Monoclonal Anti Phospho Camkii Thr286, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho camkii thr286/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti phospho camkii thr286 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "The regulation of enteric neuron connectivity by semaphorin 5A is affected by the autism-associated S956G missense mutation"

    Article Title: The regulation of enteric neuron connectivity by semaphorin 5A is affected by the autism-associated S956G missense mutation

    Journal: iScience

    doi: 10.1016/j.isci.2024.109638


    Figure Legend Snippet:

    Techniques Used: Recombinant, Western Blot, Mutagenesis, Bicinchoninic Acid Protein Assay, Software, Membrane

    rabbit monoclonal anti phospho camkii thr286  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit monoclonal anti phospho camkii thr286

    Rabbit Monoclonal Anti Phospho Camkii Thr286, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho camkii thr286/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti phospho camkii thr286 - by Bioz Stars, 2024-07
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    Images

    1) Product Images from "The regulation of enteric neuron connectivity by semaphorin 5A is affected by the autism-associated S956G missense mutation"

    Article Title: The regulation of enteric neuron connectivity by semaphorin 5A is affected by the autism-associated S956G missense mutation

    Journal: iScience

    doi: 10.1016/j.isci.2024.109638


    Figure Legend Snippet:

    Techniques Used: Recombinant, Western Blot, Mutagenesis, Bicinchoninic Acid Protein Assay, Software, Membrane

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    Cell Signaling Technology Inc phospho camkii
    JOSD2 aggravates ISO-induced myocardial injury via activation of CaMKIIδ. WT mice were infected with AAV9 carrying JOSD2 cDNA (AAV9-JOSD2) or an empty vector (AAV9-NC) through tail vein. Four weeks post-injection, mice were infused with ISO. During the ISO-infused period, KN-93 was administered at 5 mg/kg/day. A Representative M-mode echocardiographic images from mice in each group. B Representative images from harvested heart tissues (scale bar: 2.5 mm). C–I Representative images and quantification of heart tissues stained with WGA (C–D), H&E (E), Sirius Red (F–G) and Masson (H–I) in sections of hearts (scale bar: 100 μm). J The content of ANP in plasma. K mRNA levels of hypertrophy-associated genes and fibrosis-associated genes in mouse heart tissues. Data was normalized to Actb. L Western blot analysis of hypertrophy-associated β-MyHC, ANP and fibrosis-associated COL-1, TGF-β1 in heart tissues. GAPDH was used as the loading control. M Western blot analysis of p-CaMKIIδ, CaMKIIδ in heart tissues. GAPDH was used as the loading control. All quantitative data is presented as Mean ± SEM; n = 6; ns = not significant; * = p < 0.05
    Phospho Camkii, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho camkii/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    phospho camkii - by Bioz Stars, 2024-07
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    86
    Cell Signaling Technology Inc rabbit monoclonal anti phospho camkii thr286
    JOSD2 aggravates ISO-induced myocardial injury via activation of CaMKIIδ. WT mice were infected with AAV9 carrying JOSD2 cDNA (AAV9-JOSD2) or an empty vector (AAV9-NC) through tail vein. Four weeks post-injection, mice were infused with ISO. During the ISO-infused period, KN-93 was administered at 5 mg/kg/day. A Representative M-mode echocardiographic images from mice in each group. B Representative images from harvested heart tissues (scale bar: 2.5 mm). C–I Representative images and quantification of heart tissues stained with WGA (C–D), H&E (E), Sirius Red (F–G) and Masson (H–I) in sections of hearts (scale bar: 100 μm). J The content of ANP in plasma. K mRNA levels of hypertrophy-associated genes and fibrosis-associated genes in mouse heart tissues. Data was normalized to Actb. L Western blot analysis of hypertrophy-associated β-MyHC, ANP and fibrosis-associated COL-1, TGF-β1 in heart tissues. GAPDH was used as the loading control. M Western blot analysis of p-CaMKIIδ, CaMKIIδ in heart tissues. GAPDH was used as the loading control. All quantitative data is presented as Mean ± SEM; n = 6; ns = not significant; * = p < 0.05
    Rabbit Monoclonal Anti Phospho Camkii Thr286, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho camkii thr286/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti phospho camkii thr286 - by Bioz Stars, 2024-07
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    86
    Cell Signaling Technology Inc phospho camkii t286
    Lack of Ng increases <t>CaMKII</t> signaling and periostin expression in the heart. A. Heart lysates from Ng−/− mice exhibit significant increases in Ca2+-CaM signaling. B. Ng−/− mice exhibit increased trends for CaM expression in the heart lysate. C. Phospho-CaMKII expression <t>(T286)</t> is significantly increased in the heart of Ng−/− mice. D-E. There are increased trends of protein expression of pan-CaMKII and CaN. F. Lack of Ng does not affect RyR expression in the heart. G. Significantly increased periostin expression is observed in the heart of Ng−/− mice (n = 4 per genotype). H. Ng siRNA knockdown (n = 6) in HL-1 cell line exhibits altered CaMKII activity compared to those of mock (n = 3) and lipofectamine (n = 3) controls. I. Phospho-CaMKII expression (T286) was significantly increased by Ng siRNA in HL-1, while there was no change in CaMKIIδ expression. *p < 0.05.
    Phospho Camkii T286, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho camkii t286/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho camkii t286 - by Bioz Stars, 2024-07
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    86
    Cell Signaling Technology Inc phospho camkii thr286
    Lack of Ng increases <t>CaMKII</t> signaling and periostin expression in the heart. A. Heart lysates from Ng−/− mice exhibit significant increases in Ca2+-CaM signaling. B. Ng−/− mice exhibit increased trends for CaM expression in the heart lysate. C. Phospho-CaMKII expression <t>(T286)</t> is significantly increased in the heart of Ng−/− mice. D-E. There are increased trends of protein expression of pan-CaMKII and CaN. F. Lack of Ng does not affect RyR expression in the heart. G. Significantly increased periostin expression is observed in the heart of Ng−/− mice (n = 4 per genotype). H. Ng siRNA knockdown (n = 6) in HL-1 cell line exhibits altered CaMKII activity compared to those of mock (n = 3) and lipofectamine (n = 3) controls. I. Phospho-CaMKII expression (T286) was significantly increased by Ng siRNA in HL-1, while there was no change in CaMKIIδ expression. *p < 0.05.
    Phospho Camkii Thr286, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho camkii thr286/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho camkii thr286 - by Bioz Stars, 2024-07
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    Image Search Results


    JOSD2 aggravates ISO-induced myocardial injury via activation of CaMKIIδ. WT mice were infected with AAV9 carrying JOSD2 cDNA (AAV9-JOSD2) or an empty vector (AAV9-NC) through tail vein. Four weeks post-injection, mice were infused with ISO. During the ISO-infused period, KN-93 was administered at 5 mg/kg/day. A Representative M-mode echocardiographic images from mice in each group. B Representative images from harvested heart tissues (scale bar: 2.5 mm). C–I Representative images and quantification of heart tissues stained with WGA (C–D), H&E (E), Sirius Red (F–G) and Masson (H–I) in sections of hearts (scale bar: 100 μm). J The content of ANP in plasma. K mRNA levels of hypertrophy-associated genes and fibrosis-associated genes in mouse heart tissues. Data was normalized to Actb. L Western blot analysis of hypertrophy-associated β-MyHC, ANP and fibrosis-associated COL-1, TGF-β1 in heart tissues. GAPDH was used as the loading control. M Western blot analysis of p-CaMKIIδ, CaMKIIδ in heart tissues. GAPDH was used as the loading control. All quantitative data is presented as Mean ± SEM; n = 6; ns = not significant; * = p < 0.05

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: JOSD2 mediates isoprenaline-induced heart failure by deubiquitinating CaMKIIδ in cardiomyocytes

    doi: 10.1007/s00018-023-05037-7

    Figure Lengend Snippet: JOSD2 aggravates ISO-induced myocardial injury via activation of CaMKIIδ. WT mice were infected with AAV9 carrying JOSD2 cDNA (AAV9-JOSD2) or an empty vector (AAV9-NC) through tail vein. Four weeks post-injection, mice were infused with ISO. During the ISO-infused period, KN-93 was administered at 5 mg/kg/day. A Representative M-mode echocardiographic images from mice in each group. B Representative images from harvested heart tissues (scale bar: 2.5 mm). C–I Representative images and quantification of heart tissues stained with WGA (C–D), H&E (E), Sirius Red (F–G) and Masson (H–I) in sections of hearts (scale bar: 100 μm). J The content of ANP in plasma. K mRNA levels of hypertrophy-associated genes and fibrosis-associated genes in mouse heart tissues. Data was normalized to Actb. L Western blot analysis of hypertrophy-associated β-MyHC, ANP and fibrosis-associated COL-1, TGF-β1 in heart tissues. GAPDH was used as the loading control. M Western blot analysis of p-CaMKIIδ, CaMKIIδ in heart tissues. GAPDH was used as the loading control. All quantitative data is presented as Mean ± SEM; n = 6; ns = not significant; * = p < 0.05

    Article Snippet: Phospho-CaMKII (Cat# 12716 s), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Cat# 7076), and goat anti-rabbit IgG (Cat# 7074) were purchased from Cell Signaling Technology (CST, Danvers, MA, USA).

    Techniques: Activation Assay, Infection, Plasmid Preparation, Injection, Staining, Western Blot

    JOSD2 interacts CaMKIIδ directly and promotes CaMKIIδ activation. A-B MS/MS spectrum of the peptide showing FTDEYQLFEELGK and ESTESSNTTIEDEDVK from CaMKIIδ. Single-letter abbreviations: K, Lys; V, Val; I, Ile; G, Gly; Q, Gln; L, Leu; N, Asn; E, Glu; T, Thr; S, Ser; F, Phe; D, Asp; Y, Tyr. m/z, mass/charge ratio. C The complex of JOSD2 and CaMKIIδ in NRVMs were detected by Coimmunoprecipitation. NRVMs were transfected with Flag-tagged JOSD2. Lysates from cells were immunoprecipitated with an anti-Flag antibody. CaMKIIδ levels were detected by immunoblotting. IgG was used as a control for IP. D In HEK-293 T cells which express a few endogenous CaMKII, Flag-tagged JOSD2 and GFP-tagged CaMKIIδ were transfected. Lysates from cells were immunoprecipitated with anti-Flag antibody. GFP levels were detected by immunoblotting. IgG was used as a control for IP. E Representative western blot for CaMKIIδ and p-CaMKIIδ in NRVMs with gradient levels of JOSD2. F–G JOSD2 was silenced or overexpressed in NRVMs. Representative western blotting for CaMKIIδ and p-CaMKIIδ in NRVMs. GAPDH was used as a loading control. (H-I) Representative western blotting for CaMKIIδ and p-CaMKIIδ in heart tissues from ISO- and MI-induced mice. J–K Confocal images (J)and representative recordings (K) of SR Ca2+ determined by the Fluo-4 AM fluorescence signal in cardiomyocytes transfected with si-JOSD2 or si-NC before ISO stimulation for 24 h. n = 3 cells in each group. [Scale bar, 50 μm]. L–M Confocal images (L) and representative recordings (M) of SR Ca2+ determined by the Fluo-4 AM fluorescence signal in cardiomyocytes transfected with Flag-JOSD2 or empty vector (EV) before ISO stimulation for 24 h. n = 6 cells in each group. [Scale bar, 50 μm]

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: JOSD2 mediates isoprenaline-induced heart failure by deubiquitinating CaMKIIδ in cardiomyocytes

    doi: 10.1007/s00018-023-05037-7

    Figure Lengend Snippet: JOSD2 interacts CaMKIIδ directly and promotes CaMKIIδ activation. A-B MS/MS spectrum of the peptide showing FTDEYQLFEELGK and ESTESSNTTIEDEDVK from CaMKIIδ. Single-letter abbreviations: K, Lys; V, Val; I, Ile; G, Gly; Q, Gln; L, Leu; N, Asn; E, Glu; T, Thr; S, Ser; F, Phe; D, Asp; Y, Tyr. m/z, mass/charge ratio. C The complex of JOSD2 and CaMKIIδ in NRVMs were detected by Coimmunoprecipitation. NRVMs were transfected with Flag-tagged JOSD2. Lysates from cells were immunoprecipitated with an anti-Flag antibody. CaMKIIδ levels were detected by immunoblotting. IgG was used as a control for IP. D In HEK-293 T cells which express a few endogenous CaMKII, Flag-tagged JOSD2 and GFP-tagged CaMKIIδ were transfected. Lysates from cells were immunoprecipitated with anti-Flag antibody. GFP levels were detected by immunoblotting. IgG was used as a control for IP. E Representative western blot for CaMKIIδ and p-CaMKIIδ in NRVMs with gradient levels of JOSD2. F–G JOSD2 was silenced or overexpressed in NRVMs. Representative western blotting for CaMKIIδ and p-CaMKIIδ in NRVMs. GAPDH was used as a loading control. (H-I) Representative western blotting for CaMKIIδ and p-CaMKIIδ in heart tissues from ISO- and MI-induced mice. J–K Confocal images (J)and representative recordings (K) of SR Ca2+ determined by the Fluo-4 AM fluorescence signal in cardiomyocytes transfected with si-JOSD2 or si-NC before ISO stimulation for 24 h. n = 3 cells in each group. [Scale bar, 50 μm]. L–M Confocal images (L) and representative recordings (M) of SR Ca2+ determined by the Fluo-4 AM fluorescence signal in cardiomyocytes transfected with Flag-JOSD2 or empty vector (EV) before ISO stimulation for 24 h. n = 6 cells in each group. [Scale bar, 50 μm]

    Article Snippet: Phospho-CaMKII (Cat# 12716 s), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Cat# 7076), and goat anti-rabbit IgG (Cat# 7074) were purchased from Cell Signaling Technology (CST, Danvers, MA, USA).

    Techniques: Activation Assay, Tandem Mass Spectroscopy, Transfection, Immunoprecipitation, Western Blot, Fluorescence, Plasmid Preparation

    Lack of Ng increases CaMKII signaling and periostin expression in the heart. A. Heart lysates from Ng−/− mice exhibit significant increases in Ca2+-CaM signaling. B. Ng−/− mice exhibit increased trends for CaM expression in the heart lysate. C. Phospho-CaMKII expression (T286) is significantly increased in the heart of Ng−/− mice. D-E. There are increased trends of protein expression of pan-CaMKII and CaN. F. Lack of Ng does not affect RyR expression in the heart. G. Significantly increased periostin expression is observed in the heart of Ng−/− mice (n = 4 per genotype). H. Ng siRNA knockdown (n = 6) in HL-1 cell line exhibits altered CaMKII activity compared to those of mock (n = 3) and lipofectamine (n = 3) controls. I. Phospho-CaMKII expression (T286) was significantly increased by Ng siRNA in HL-1, while there was no change in CaMKIIδ expression. *p < 0.05.

    Journal: Experimental and molecular pathology

    Article Title: Neurogranin regulates calcium-dependent cardiac hypertrophy

    doi: 10.1016/j.yexmp.2022.104815

    Figure Lengend Snippet: Lack of Ng increases CaMKII signaling and periostin expression in the heart. A. Heart lysates from Ng−/− mice exhibit significant increases in Ca2+-CaM signaling. B. Ng−/− mice exhibit increased trends for CaM expression in the heart lysate. C. Phospho-CaMKII expression (T286) is significantly increased in the heart of Ng−/− mice. D-E. There are increased trends of protein expression of pan-CaMKII and CaN. F. Lack of Ng does not affect RyR expression in the heart. G. Significantly increased periostin expression is observed in the heart of Ng−/− mice (n = 4 per genotype). H. Ng siRNA knockdown (n = 6) in HL-1 cell line exhibits altered CaMKII activity compared to those of mock (n = 3) and lipofectamine (n = 3) controls. I. Phospho-CaMKII expression (T286) was significantly increased by Ng siRNA in HL-1, while there was no change in CaMKIIδ expression. *p < 0.05.

    Article Snippet: Cytoplasmic proteins were separated using 4–12% SDS-PAGE (BioRad, Hercules, CA) at 100 V for 1.5 h, transferred onto PVDF membranes at 25 V for 7 min using a Trans-Blot Turbo Transfer System (BioRad, Hercules, CA), and incubated with antibodies against Neurogranin #07–425-I, Calcineurin #07–1490, and Ryanodine Receptor 2 #AB9080 (Millipore, Billerica, MA), Periostin #PA5–34641 (Invitrogen, Carlsbad, CA, USA), Calmodulin #4830S, Phospho-CaMKII (T286) #12716S, and CaMKII (pan) #4436S (Cell Signaling, Danvers, MA), and GAPDH #SC-32233 (Santa Cruz, Dallas, TX).

    Techniques: Expressing, Activity Assay