Journal: eLife

Article Title: ACE2 pathway regulates thermogenesis and energy metabolism

doi: 10.7554/eLife.72266

Figure Lengend Snippet: Primary brown adipocytes were isolated, cultured, and treated with Ang-(1-7) (10 –6 M) for 24 hr, Akt inhibitor MK2206 (20 μM) for 24 hr, PKA inhibitor H89 (30 μM) for 24 hr, or adenylylcyclase inhibitor SQ-22536 (10 μM) for 24 hours. ( A, B ) Representative western blots showing the changes of p-Akt308 and Akt in BAT of Ace2 -/y ( A ) and Lepr db/db + Ace2 mice ( B ) exposure at 4 °C (n = 3/each group). ( C ) Representative western blots showing the Akt, UCP1 and forkhead box protein O 1 (FoxO1) Changes (n = 3/each group). ( D ) Relative mRNA levels of thermogenic and mitochondrial genes (n = 4–6/each group). ( E ) Continuous measurement of oxygen consumption rate (OCR) in Ang-(1-7) and MK2206 treated primary brown adipocytes. Oxygen consumption was performed under basal conditions, following the addition of oligomycin (1 μM), the pharmacological uncoupler FCCP (1 μM) or the Complex III and I inhibitor antimycin A and rotenone (0.5 μM) (n = 3–4/each group). ( F, G ) Representative western blots showing the p-PKA and PKA changes in BAT of Ace2 -/y ( F ) and Lepr db/db + Ace2 mice ( G ) exposure at 4 °C (n = 3/each group). ( H ) Relative mRNA levels of thermogenic and mitochondrial genes (n = 4–6/each group). ( I ) Representative western blots showing the UCP1 and PGC1α changes (n = 3/each group). ( J ) Continuous measurement of OCR in Ang-(1-7) and H89-treated primary brown Adipocytes (n = 3–5/each group). Data represent mean ± SEM. *p< 0.05, **p < 0.01 vs GFP/WT group by Student’s t -test. *p < 0.05, **p < 0.01 vs control group, # p < 0.05, ## p < 0.01 vs Ang-(1-7) group by one-way ANOVA. ( K ) Mechanisms involved in ACE2 pathway activation-induced improvement of BAT function.

Article Snippet: Antibody , Anti-p-Akt308 (rabbit monoclonal) , Cell signaling , #13038 RRID: AB_2629447 , (1:1000).

Techniques: Isolation, Cell Culture, Western Blot, Activation Assay

Journal: eLife

Article Title: ACE2 pathway regulates thermogenesis and energy metabolism

doi: 10.7554/eLife.72266

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-p-Akt308 (rabbit monoclonal) , Cell signaling , #13038 RRID: AB_2629447 , (1:1000).

Techniques: Transfection, Construct, Sequencing, Software

Journal: Translational Cancer Research

Article Title: ET-1 promotes the growth and metastasis of esophageal squamous cell carcinoma via activating PI3K/Akt pathway

doi: 10.21037/tcr.2020.04.26

Figure Lengend Snippet: Knockdown of ET-1 blocked the PI3K/Akt pathway. Western blot was performed to detect the level of p-PI3K, PI3K, p-Akt473, p-Akt308 and Akt in ECA109 cells. ***, P<0.001, compared with the control group; ### , P<0.001, compared with the si-ET-1 group.

Article Snippet: The polyvinylidene fluoride (PVDF) membranes were incubated overnight at 4 °C with the primary antibodies ET-1 (1:1,000, #ab117529, Abcam, USA), p-PI3K (1:1,000, #17366, Cell Signaling Technology, USA), PI3K (1:1,000, #4249, Cell Signaling Technology, USA), p-Akt473 (1:2,000, #4060, Cell Signaling Technology, USA), p-Akt308 (1:1,000, #13038, Cell Signaling Technology, USA), Akt (1:1,000, #4685, Cell Signaling Technology, USA) and β-actin (1:1,000, #ab5694, Abcam, USA).

Techniques: Western Blot