phospho akt ser473  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho akt ser473
    Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phospho akt ser473 587f11 mouse mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho akt ser473 587f11 mouse mab
    Phospho Akt Ser473 587f11 Mouse Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phospho akt ser473  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho akt ser473
    Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    polyclonal antibodies against phospho akt ser473 p akt  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc polyclonal antibodies against phospho akt ser473 p akt
    4A. Western blot analysis of AKT, <t>phospho-AKT</t> <t>(Ser473)</t> <t>(p-AKT</t> [Ser473]), ERK1/2, and phospho-ERK1/2 (p-ERK1/2) expressions in NRAS Q61R and NRAS WT HDLECs. 4B. Multiplex protein assay of p-AKT (a), p-mTOR (b), p-ERK (c), in NRAS Q61R and NRAS WT HDLEC mixtures. Bars represent the mean ± SD of triplicate wells. *p<0.05, **p<0.01, ***p<0.001, compared with 0%. 4C. Multiplex protein assay of AKT (a), p-mTOR (b), and p-ERK(c) in NRAS Q61R HDLECs with or without treatment with sirolimus and trametinib. Bars represent the mean ± SD of triplicate wells. *p<0.05, **p<0.01, ***p<0.001, compared with no treatment. After sirolimus and trametinib treatment, the absorbance of NRAS Q61R HDLECs treated with sirolimus and trametinib decreased significantly compared with that of cells with no treatment.
    Polyclonal Antibodies Against Phospho Akt Ser473 P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Changes in cell morphology and function induced by the NRAS Q61R mutation in lymphatic endothelial cells"

    Article Title: Changes in cell morphology and function induced by the NRAS Q61R mutation in lymphatic endothelial cells

    Journal: PLOS ONE

    doi: 10.1371/journal.pone.0289187

    4A. Western blot analysis of AKT, phospho-AKT (Ser473) (p-AKT [Ser473]), ERK1/2, and phospho-ERK1/2 (p-ERK1/2) expressions in NRAS Q61R and NRAS WT HDLECs. 4B. Multiplex protein assay of p-AKT (a), p-mTOR (b), p-ERK (c), in NRAS Q61R and NRAS WT HDLEC mixtures. Bars represent the mean ± SD of triplicate wells. *p<0.05, **p<0.01, ***p<0.001, compared with 0%. 4C. Multiplex protein assay of AKT (a), p-mTOR (b), and p-ERK(c) in NRAS Q61R HDLECs with or without treatment with sirolimus and trametinib. Bars represent the mean ± SD of triplicate wells. *p<0.05, **p<0.01, ***p<0.001, compared with no treatment. After sirolimus and trametinib treatment, the absorbance of NRAS Q61R HDLECs treated with sirolimus and trametinib decreased significantly compared with that of cells with no treatment.
    Figure Legend Snippet: 4A. Western blot analysis of AKT, phospho-AKT (Ser473) (p-AKT [Ser473]), ERK1/2, and phospho-ERK1/2 (p-ERK1/2) expressions in NRAS Q61R and NRAS WT HDLECs. 4B. Multiplex protein assay of p-AKT (a), p-mTOR (b), p-ERK (c), in NRAS Q61R and NRAS WT HDLEC mixtures. Bars represent the mean ± SD of triplicate wells. *p<0.05, **p<0.01, ***p<0.001, compared with 0%. 4C. Multiplex protein assay of AKT (a), p-mTOR (b), and p-ERK(c) in NRAS Q61R HDLECs with or without treatment with sirolimus and trametinib. Bars represent the mean ± SD of triplicate wells. *p<0.05, **p<0.01, ***p<0.001, compared with no treatment. After sirolimus and trametinib treatment, the absorbance of NRAS Q61R HDLECs treated with sirolimus and trametinib decreased significantly compared with that of cells with no treatment.

    Techniques Used: Western Blot, Multiplex Assay

    phospho akt ser473  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho akt ser473
    Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho akt ser473/product/Cell Signaling Technology Inc
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    polyclonal antibodies against phospho akt ser473 p akt  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc polyclonal antibodies against phospho akt ser473 p akt
    Polyclonal Antibodies Against Phospho Akt Ser473 P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibodies against phospho akt ser473 p akt/product/Cell Signaling Technology Inc
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    phospho akt ser473  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho akt ser473
    Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phospho akt ser473  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho akt ser473
    Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phospho akt ser473  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho akt ser473
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    phospho akt ser473  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho akt ser473
    A. In vivo insulin stimulation of AKT <t>Ser473</t> phosphorylation in indicated tissues (phospho-AKT Ser473 over total-AKT). Male mice were fasted 4 hours prior to insulin injection. N = 6 for both groups. B. Phospho-proteomic analysis of in vivo insulin-stimulated liver tissues in (A). Left: Scattered plot of the analysis with indicated phosphor-proteins. Right: Heat map of averaged phosphor-proteins. N = 6 from control and N = 5 from KO liver, male mice. C. PDH activity in response to insulin in (A). N = 4 per group per each tissue. D. Metabolomics in serum from high-fat diet fed control and MBC UCP1 KO male mice. N = 6 for control and 7 for MBC UCP1 KO. E. Oxidative stress marker protein carbonyl content in the liver from control and MBC UCP1 KO male mice on a high-fat diet. N = 6 per group. F. Abundance of lipid oxidative stress marker malondialdehyde (MDA) content (left) and 4-hydroxylnonenal (4-HNE) content (right) in liver tissue. N = 5 per group for MDA content, N = 6 per group for 4-HNE quantification. G. Insulin tolerance test and AUC of high-fat diet fed control and MBC UCP1 KO male mice prior to glutathione supplementation. Mice were fasted for 4 hours prior to intraperitoneal injection of insulin (1 U kg −1 ). N = 9 control and 6 for MBC UCP KO mice. H. Insulin tolerance test and AUC of male mice in (G) following 10 days of glutathione supplementation (2 g kg −1 ). Mice received glutathione supplementation 18 hours prior to insulin tolerance test to limit acute glutathione response. N = 7 control mice and 6 for MBC UCP KO mice. I. Serum GSH levels in wild-type male mice following BSO treatment. N = 9 per group. J. Insulin tolerance test (0.5 U kg −1 ) in wild-type male mice treated with BSO for 20 days. N = 9 per group. K. Serum BCAA levels in (J). L. Correlation between serum BCAA and serum GSH levels in (J).
    Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "BCAA nitrogen flux in brown fat controls metabolic health independent of thermogenesis"

    Article Title: BCAA nitrogen flux in brown fat controls metabolic health independent of thermogenesis

    Journal: Cell

    doi: 10.1016/j.cell.2024.03.030

    A. In vivo insulin stimulation of AKT Ser473 phosphorylation in indicated tissues (phospho-AKT Ser473 over total-AKT). Male mice were fasted 4 hours prior to insulin injection. N = 6 for both groups. B. Phospho-proteomic analysis of in vivo insulin-stimulated liver tissues in (A). Left: Scattered plot of the analysis with indicated phosphor-proteins. Right: Heat map of averaged phosphor-proteins. N = 6 from control and N = 5 from KO liver, male mice. C. PDH activity in response to insulin in (A). N = 4 per group per each tissue. D. Metabolomics in serum from high-fat diet fed control and MBC UCP1 KO male mice. N = 6 for control and 7 for MBC UCP1 KO. E. Oxidative stress marker protein carbonyl content in the liver from control and MBC UCP1 KO male mice on a high-fat diet. N = 6 per group. F. Abundance of lipid oxidative stress marker malondialdehyde (MDA) content (left) and 4-hydroxylnonenal (4-HNE) content (right) in liver tissue. N = 5 per group for MDA content, N = 6 per group for 4-HNE quantification. G. Insulin tolerance test and AUC of high-fat diet fed control and MBC UCP1 KO male mice prior to glutathione supplementation. Mice were fasted for 4 hours prior to intraperitoneal injection of insulin (1 U kg −1 ). N = 9 control and 6 for MBC UCP KO mice. H. Insulin tolerance test and AUC of male mice in (G) following 10 days of glutathione supplementation (2 g kg −1 ). Mice received glutathione supplementation 18 hours prior to insulin tolerance test to limit acute glutathione response. N = 7 control mice and 6 for MBC UCP KO mice. I. Serum GSH levels in wild-type male mice following BSO treatment. N = 9 per group. J. Insulin tolerance test (0.5 U kg −1 ) in wild-type male mice treated with BSO for 20 days. N = 9 per group. K. Serum BCAA levels in (J). L. Correlation between serum BCAA and serum GSH levels in (J).
    Figure Legend Snippet: A. In vivo insulin stimulation of AKT Ser473 phosphorylation in indicated tissues (phospho-AKT Ser473 over total-AKT). Male mice were fasted 4 hours prior to insulin injection. N = 6 for both groups. B. Phospho-proteomic analysis of in vivo insulin-stimulated liver tissues in (A). Left: Scattered plot of the analysis with indicated phosphor-proteins. Right: Heat map of averaged phosphor-proteins. N = 6 from control and N = 5 from KO liver, male mice. C. PDH activity in response to insulin in (A). N = 4 per group per each tissue. D. Metabolomics in serum from high-fat diet fed control and MBC UCP1 KO male mice. N = 6 for control and 7 for MBC UCP1 KO. E. Oxidative stress marker protein carbonyl content in the liver from control and MBC UCP1 KO male mice on a high-fat diet. N = 6 per group. F. Abundance of lipid oxidative stress marker malondialdehyde (MDA) content (left) and 4-hydroxylnonenal (4-HNE) content (right) in liver tissue. N = 5 per group for MDA content, N = 6 per group for 4-HNE quantification. G. Insulin tolerance test and AUC of high-fat diet fed control and MBC UCP1 KO male mice prior to glutathione supplementation. Mice were fasted for 4 hours prior to intraperitoneal injection of insulin (1 U kg −1 ). N = 9 control and 6 for MBC UCP KO mice. H. Insulin tolerance test and AUC of male mice in (G) following 10 days of glutathione supplementation (2 g kg −1 ). Mice received glutathione supplementation 18 hours prior to insulin tolerance test to limit acute glutathione response. N = 7 control mice and 6 for MBC UCP KO mice. I. Serum GSH levels in wild-type male mice following BSO treatment. N = 9 per group. J. Insulin tolerance test (0.5 U kg −1 ) in wild-type male mice treated with BSO for 20 days. N = 9 per group. K. Serum BCAA levels in (J). L. Correlation between serum BCAA and serum GSH levels in (J).

    Techniques Used: In Vivo, Injection, Activity Assay, Marker

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Protease Inhibitor, Activity Assay, Reverse Transcription, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Software

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    Cell Signaling Technology Inc phospho akt ser473
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    Cell Signaling Technology Inc phospho akt ser473 587f11 mouse mab
    Phospho Akt Ser473 587f11 Mouse Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc polyclonal antibodies against phospho akt ser473 p akt
    4A. Western blot analysis of AKT, <t>phospho-AKT</t> <t>(Ser473)</t> <t>(p-AKT</t> [Ser473]), ERK1/2, and phospho-ERK1/2 (p-ERK1/2) expressions in NRAS Q61R and NRAS WT HDLECs. 4B. Multiplex protein assay of p-AKT (a), p-mTOR (b), p-ERK (c), in NRAS Q61R and NRAS WT HDLEC mixtures. Bars represent the mean ± SD of triplicate wells. *p<0.05, **p<0.01, ***p<0.001, compared with 0%. 4C. Multiplex protein assay of AKT (a), p-mTOR (b), and p-ERK(c) in NRAS Q61R HDLECs with or without treatment with sirolimus and trametinib. Bars represent the mean ± SD of triplicate wells. *p<0.05, **p<0.01, ***p<0.001, compared with no treatment. After sirolimus and trametinib treatment, the absorbance of NRAS Q61R HDLECs treated with sirolimus and trametinib decreased significantly compared with that of cells with no treatment.
    Polyclonal Antibodies Against Phospho Akt Ser473 P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    4A. Western blot analysis of AKT, phospho-AKT (Ser473) (p-AKT [Ser473]), ERK1/2, and phospho-ERK1/2 (p-ERK1/2) expressions in NRAS Q61R and NRAS WT HDLECs. 4B. Multiplex protein assay of p-AKT (a), p-mTOR (b), p-ERK (c), in NRAS Q61R and NRAS WT HDLEC mixtures. Bars represent the mean ± SD of triplicate wells. *p<0.05, **p<0.01, ***p<0.001, compared with 0%. 4C. Multiplex protein assay of AKT (a), p-mTOR (b), and p-ERK(c) in NRAS Q61R HDLECs with or without treatment with sirolimus and trametinib. Bars represent the mean ± SD of triplicate wells. *p<0.05, **p<0.01, ***p<0.001, compared with no treatment. After sirolimus and trametinib treatment, the absorbance of NRAS Q61R HDLECs treated with sirolimus and trametinib decreased significantly compared with that of cells with no treatment.

    Journal: PLOS ONE

    Article Title: Changes in cell morphology and function induced by the NRAS Q61R mutation in lymphatic endothelial cells

    doi: 10.1371/journal.pone.0289187

    Figure Lengend Snippet: 4A. Western blot analysis of AKT, phospho-AKT (Ser473) (p-AKT [Ser473]), ERK1/2, and phospho-ERK1/2 (p-ERK1/2) expressions in NRAS Q61R and NRAS WT HDLECs. 4B. Multiplex protein assay of p-AKT (a), p-mTOR (b), p-ERK (c), in NRAS Q61R and NRAS WT HDLEC mixtures. Bars represent the mean ± SD of triplicate wells. *p<0.05, **p<0.01, ***p<0.001, compared with 0%. 4C. Multiplex protein assay of AKT (a), p-mTOR (b), and p-ERK(c) in NRAS Q61R HDLECs with or without treatment with sirolimus and trametinib. Bars represent the mean ± SD of triplicate wells. *p<0.05, **p<0.01, ***p<0.001, compared with no treatment. After sirolimus and trametinib treatment, the absorbance of NRAS Q61R HDLECs treated with sirolimus and trametinib decreased significantly compared with that of cells with no treatment.

    Article Snippet: Monoclonal antibodies against AKT (2920S) and polyclonal antibodies against phospho-AKT (Ser473) (p-AKT [Ser473]; 9271S) were purchased from Cell Signaling Technology (Boston, MA, USA).

    Techniques: Western Blot, Multiplex Assay