Journal: Cell Reports

Article Title: A structural blueprint for interleukin-21 signal modulation

doi: 10.1016/j.celrep.2023.112657

Figure Lengend Snippet:

Article Snippet: Anti- phospho Akt (Ser473) (D9E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) , Cell Signaling Technologies , Cat#4075S; RRID: AB_916029.

Techniques: Blocking Assay, Purification, Recombinant, Lysis, Cell Culture, Expressing, Transfection, Enzyme-linked Immunosorbent Assay, Cell Isolation, Electron Microscopy, Software

Journal: bioRxiv

Article Title: ATP citrate lyase is an essential player of the metabolic rewiring induced by PTEN loss during T-ALL development

doi: 10.1101/2023.03.27.534353

Figure Lengend Snippet: (A) Principal component analysis of 4 pten mutant and 5 pten flox2 thymi issued from pre-leukemic 8 weeks old mice subjected to unsupervised metabolic analysis. (B) Heatmaps of the 50 most deregulated metabolites and (C) analysis of citrate, coenzyme A, glutamine, a-ketoglutarate, acetyl-coA and fatty acids in pre-leukemic PTEN Δ/Δ thymocytes or control counterparts . (D) Representative western blot analysis of phosphorylated ACLY (Ser455) and phoshorylated AKT (Ser473) in thymi isolated from 8 weeks old, pre-leukemic PTEN Δ/Δ mutant mice or PTEN flox2 control counterparts. (E) Histograms depicting phosphorylated ACLY levels (ser455) in the thymi of pre-leukemic PTEN Δ/Δ mutant or control animals in 3 independent experiments (data are shown as average ± SE).

Article Snippet: Protein samples were allowed to migrated on 4-20% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad Cat. 4561094) and then transferred on nitrocellulose membranes (Bio-Rad Cat. 1704158), which were subsequently incubated overnight at 4°C with the following primary antibodies: anti ATP citrate lysase antibody D1X6P (Cell Signaling 13390S), Anti Phospho ATP citrate Lysase antibody Ser455 (Cell Signaling 4331S), anti PTEN antibody (Cell Signaling 9188S), Anti Phospho-Akt Ser473 (D9E) XP® (Cell Signaling 4060S), Anti AKT antibody (Cell signaling 9272S) and anti-actin antibody (Abcam ab-14128).

Techniques: Mutagenesis, Western Blot, Isolation

Journal: bioRxiv

Article Title: ATP citrate lyase is an essential player of the metabolic rewiring induced by PTEN loss during T-ALL development

doi: 10.1101/2023.03.27.534353

Figure Lengend Snippet: (A) Phospho AKT (ser473) and (B) and phospho ACLY (ser455) levels on DN, DP, SP4 and SP8 from pre-leukemic PTEN Δ/Δ mutant or PTEN flox2 control counterparts. In mutant mice, which carried the YFP flox2 reporter, DN, DP, SP4 and SP8 cells are gated on YFP+ cells. Levels of Bcl-xL (C) and BCL-2 (D) in DP cells from PTEN Δ/Δ mutant, ACLY Δ/Δ mutant, PTEN Δ/Δ ;ACLY Δ/Δ double mutant mice or flox control animals (E) Sensitivity of DP cells from PTEN Δ/Δ mutant, ACLY Δ/Δ mutant, PTEN Δ/Δ ;ACLY Δ/Δ double mutant mice or flox control animals to dexamethasone treatment ex vivo. (F) Percentages of DP cells in PTEN Δ/Δ mutant, ACLY Δ/Δ mutant, PTEN Δ/Δ ;ACLY Δ/Δ double mutant mice or flox control animals receiving PBS or dexamethasone.

Article Snippet: Protein samples were allowed to migrated on 4-20% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad Cat. 4561094) and then transferred on nitrocellulose membranes (Bio-Rad Cat. 1704158), which were subsequently incubated overnight at 4°C with the following primary antibodies: anti ATP citrate lysase antibody D1X6P (Cell Signaling 13390S), Anti Phospho ATP citrate Lysase antibody Ser455 (Cell Signaling 4331S), anti PTEN antibody (Cell Signaling 9188S), Anti Phospho-Akt Ser473 (D9E) XP® (Cell Signaling 4060S), Anti AKT antibody (Cell signaling 9272S) and anti-actin antibody (Abcam ab-14128).

Techniques: Mutagenesis, Ex Vivo