phosphate buffered solution pbs  (Beyotime)

 
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    Name:
    GTP|Guanosine 5 triphosphate sodium salt hydrate
    Description:

    Catalog Number:
    ST1362-25MG
    Price:
    62.0
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    Structured Review

    Beyotime phosphate buffered solution pbs
    Reducing Ezrin suppressed metastasis of <t>EGF-injected</t> MG63 and U20S xenografts of BALB/c-nu mice. ( A ) BALB/c-mu mouse with osteosarcoma xenografts. ( B ) Liver and lung weights of tumorigenic mice. ( C ) HPRT mRNA level in livers and lungs of tumorigenic mice. ( D ) E-cadherin, vimentin, and p65 protein levels in livers and lungs of tumorigenic mice. (HPRT, hypoxanthine guanine phosphoribosyl transferase; <t>Con.+PBS,</t> tumor xenografts and PBS injection; siRNA+PBS, tumor xenografts with silencing Ezrin and PBS injection; Con.+EGF, tumor xenografts and EGF injection; siRNA+EGF, tumor xenografts with silencing Ezrin and EGF injection; * compared to Con.+PBS, P

    https://www.bioz.com/result/phosphate buffered solution pbs/product/Beyotime
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphate buffered solution pbs - by Bioz Stars, 2021-04
    86/100 stars

    Images

    1) Product Images from "Ezrin/NF-κB Pathway Regulates EGF-induced Epithelial-Mesenchymal Transition (EMT), Metastasis, and Progression of Osteosarcoma"

    Article Title: Ezrin/NF-κB Pathway Regulates EGF-induced Epithelial-Mesenchymal Transition (EMT), Metastasis, and Progression of Osteosarcoma

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    doi: 10.12659/MSM.906945

    Reducing Ezrin suppressed metastasis of EGF-injected MG63 and U20S xenografts of BALB/c-nu mice. ( A ) BALB/c-mu mouse with osteosarcoma xenografts. ( B ) Liver and lung weights of tumorigenic mice. ( C ) HPRT mRNA level in livers and lungs of tumorigenic mice. ( D ) E-cadherin, vimentin, and p65 protein levels in livers and lungs of tumorigenic mice. (HPRT, hypoxanthine guanine phosphoribosyl transferase; Con.+PBS, tumor xenografts and PBS injection; siRNA+PBS, tumor xenografts with silencing Ezrin and PBS injection; Con.+EGF, tumor xenografts and EGF injection; siRNA+EGF, tumor xenografts with silencing Ezrin and EGF injection; * compared to Con.+PBS, P
    Figure Legend Snippet: Reducing Ezrin suppressed metastasis of EGF-injected MG63 and U20S xenografts of BALB/c-nu mice. ( A ) BALB/c-mu mouse with osteosarcoma xenografts. ( B ) Liver and lung weights of tumorigenic mice. ( C ) HPRT mRNA level in livers and lungs of tumorigenic mice. ( D ) E-cadherin, vimentin, and p65 protein levels in livers and lungs of tumorigenic mice. (HPRT, hypoxanthine guanine phosphoribosyl transferase; Con.+PBS, tumor xenografts and PBS injection; siRNA+PBS, tumor xenografts with silencing Ezrin and PBS injection; Con.+EGF, tumor xenografts and EGF injection; siRNA+EGF, tumor xenografts with silencing Ezrin and EGF injection; * compared to Con.+PBS, P

    Techniques Used: Injection, Mouse Assay

    Related Articles

    Mouse Assay:

    Article Title: Ezrin/NF-κB Pathway Regulates EGF-induced Epithelial-Mesenchymal Transition (EMT), Metastasis, and Progression of Osteosarcoma
    Article Snippet: MG63 and U20S cells with silencing Ezrin were injected subcutaneously into the armpits of the mice according to the method described in a previous report [ ]. .. When the mice developed palpable xenografts (more than 0.5 cm diameter), we injected the tumor with phosphate-buffered solution (PBS) (Beyotime) or EGF (0.02 mg/kg) twice a week for 5 weeks. .. Eight weeks later, tumor xenografts were extracted, weighed, measured, and frozen in −178°C liquid nitrogen or harvested with tissue lysate buffer (Beyotime).

    Injection:

    Article Title: Ezrin/NF-κB Pathway Regulates EGF-induced Epithelial-Mesenchymal Transition (EMT), Metastasis, and Progression of Osteosarcoma
    Article Snippet: MG63 and U20S cells with silencing Ezrin were injected subcutaneously into the armpits of the mice according to the method described in a previous report [ ]. .. When the mice developed palpable xenografts (more than 0.5 cm diameter), we injected the tumor with phosphate-buffered solution (PBS) (Beyotime) or EGF (0.02 mg/kg) twice a week for 5 weeks. .. Eight weeks later, tumor xenografts were extracted, weighed, measured, and frozen in −178°C liquid nitrogen or harvested with tissue lysate buffer (Beyotime).

    other:

    Article Title: Fugan Wan alleviates hepatic fibrosis by inhibiting ACE/Ang II/AT-1R signaling pathway and enhancing ACE2/Ang 1-7/Mas signaling pathway in hepatic fibrosis rat models
    Article Snippet: The rats were anaesthetized using 3% pentobarbital sodium (Beyotime Biotech.

    Fluorescence:

    Article Title: Evaluation of plasma cytokine protein array profile: the highlighted PDGF-BB in rheumatoid arthritis.
    Article Snippet: The cytokines play critical roles in the complex pathogenesis of rheumatoid arthritis (RA), but the specific cytokines are still in need of being discovered. .. The cytokines play critical roles in the complex pathogenesis of rheumatoid arthritis (RA), but the specific cytokines are still in need of being discovered. .. The cytokines play critical roles in the complex pathogenesis of rheumatoid arthritis (RA), but the specific cytokines are still in need of being discovered.

    FACS:

    Article Title: Evaluation of plasma cytokine protein array profile: the highlighted PDGF-BB in rheumatoid arthritis.
    Article Snippet: The cytokines play critical roles in the complex pathogenesis of rheumatoid arthritis (RA), but the specific cytokines are still in need of being discovered. .. The cytokines play critical roles in the complex pathogenesis of rheumatoid arthritis (RA), but the specific cytokines are still in need of being discovered. .. The cytokines play critical roles in the complex pathogenesis of rheumatoid arthritis (RA), but the specific cytokines are still in need of being discovered.

    Protein Concentration:

    Article Title: Zishenwan Decreases Kidney Damage in Recurrent Urinary Tract Infection through the Inhibition of Toll-Like Receptor 4 Signal
    Article Snippet: Briefly, cells were collected, washed with PBS, and resuspended in 200 μ L binding buffer containing 5 μ L annexin V (10 μ g/mL) and 10 μ L PI (20 μ g/mL) for 30 min. Then samples were immediately analyzed using flow cytometry. .. Rat bladder smooth muscle cells and homogenate treated samples of tissues were lysed in RIPA (Beyotime, Shanghai, China) and the protein concentration was determined by a Bradford kit (Beyotime, Shanghai, China). .. The proteins were treated with SDS sample buffer at 100°C for 10 min and resolved by SDS-polyacrylamide gel electrophoresis (10% acrylamide) and transferred to polyvinylidene fluoride (PVDF) membrane.

    Western Blot:

    Article Title: Homozygous mutations in DZIP1 can induce asthenoteratospermia with severe MMAF
    Article Snippet: The empty and recombinant plasmids were transfected into HEK293T cells using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s recommendations. .. Western blottingTransfected cells and half of a mouse testis were homogenised in 200 µL RIPA (Beyotime) using a pellet pestle motor homogeniser and then heated at 100°C for 15 min. Lysates were fractionated by SDS-PAGE on 10% polyacrylamide gels, transferred to polyvinylidene fluoride membranes, and the membranes were blocked in TBST (3% Bovine serum albumin in tris-buffered saline with Tween-20) for 1 hour at room temperature (RT). .. Anti-DZIP1 antibody (mouse monoclonal, Santa Cruz) was diluted 1:1000 in TBST and incubated with the membranes overnight at 4°C.

    SDS Page:

    Article Title: Homozygous mutations in DZIP1 can induce asthenoteratospermia with severe MMAF
    Article Snippet: The empty and recombinant plasmids were transfected into HEK293T cells using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s recommendations. .. Western blottingTransfected cells and half of a mouse testis were homogenised in 200 µL RIPA (Beyotime) using a pellet pestle motor homogeniser and then heated at 100°C for 15 min. Lysates were fractionated by SDS-PAGE on 10% polyacrylamide gels, transferred to polyvinylidene fluoride membranes, and the membranes were blocked in TBST (3% Bovine serum albumin in tris-buffered saline with Tween-20) for 1 hour at room temperature (RT). .. Anti-DZIP1 antibody (mouse monoclonal, Santa Cruz) was diluted 1:1000 in TBST and incubated with the membranes overnight at 4°C.

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  • 93
    Beyotime phosphate buffered solution pbs
    Reducing Ezrin suppressed metastasis of <t>EGF-injected</t> MG63 and U20S xenografts of BALB/c-nu mice. ( A ) BALB/c-mu mouse with osteosarcoma xenografts. ( B ) Liver and lung weights of tumorigenic mice. ( C ) HPRT mRNA level in livers and lungs of tumorigenic mice. ( D ) E-cadherin, vimentin, and p65 protein levels in livers and lungs of tumorigenic mice. (HPRT, hypoxanthine guanine phosphoribosyl transferase; <t>Con.+PBS,</t> tumor xenografts and PBS injection; siRNA+PBS, tumor xenografts with silencing Ezrin and PBS injection; Con.+EGF, tumor xenografts and EGF injection; siRNA+EGF, tumor xenografts with silencing Ezrin and EGF injection; * compared to Con.+PBS, P
    Phosphate Buffered Solution Pbs, supplied by Beyotime, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffered solution pbs/product/Beyotime
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphate buffered solution pbs - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

    99
    Beyotime pbs solution
    Chemical structures and characterizations of untargeted and targeted <t>SHK</t> nanoformulations. Morphologies and R h s of (A) NG/SHK and (B) STP-NG/SHK. (C) Release profiles of SHK from NG/SHK and STP-NG/SHK in <t>PBS</t> without or with 10.0 mM GSH.
    Pbs Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs solution/product/Beyotime
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbs solution - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    98
    Beyotime mtt solution
    Chemical structures and characterizations of untargeted and targeted <t>SHK</t> nanoformulations. Morphologies and R h s of (A) NG/SHK and (B) STP-NG/SHK. (C) Release profiles of SHK from NG/SHK and STP-NG/SHK in <t>PBS</t> without or with 10.0 mM GSH.
    Mtt Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mtt solution/product/Beyotime
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mtt solution - by Bioz Stars, 2021-04
    98/100 stars
      Buy from Supplier

    Image Search Results


    Reducing Ezrin suppressed metastasis of EGF-injected MG63 and U20S xenografts of BALB/c-nu mice. ( A ) BALB/c-mu mouse with osteosarcoma xenografts. ( B ) Liver and lung weights of tumorigenic mice. ( C ) HPRT mRNA level in livers and lungs of tumorigenic mice. ( D ) E-cadherin, vimentin, and p65 protein levels in livers and lungs of tumorigenic mice. (HPRT, hypoxanthine guanine phosphoribosyl transferase; Con.+PBS, tumor xenografts and PBS injection; siRNA+PBS, tumor xenografts with silencing Ezrin and PBS injection; Con.+EGF, tumor xenografts and EGF injection; siRNA+EGF, tumor xenografts with silencing Ezrin and EGF injection; * compared to Con.+PBS, P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Ezrin/NF-κB Pathway Regulates EGF-induced Epithelial-Mesenchymal Transition (EMT), Metastasis, and Progression of Osteosarcoma

    doi: 10.12659/MSM.906945

    Figure Lengend Snippet: Reducing Ezrin suppressed metastasis of EGF-injected MG63 and U20S xenografts of BALB/c-nu mice. ( A ) BALB/c-mu mouse with osteosarcoma xenografts. ( B ) Liver and lung weights of tumorigenic mice. ( C ) HPRT mRNA level in livers and lungs of tumorigenic mice. ( D ) E-cadherin, vimentin, and p65 protein levels in livers and lungs of tumorigenic mice. (HPRT, hypoxanthine guanine phosphoribosyl transferase; Con.+PBS, tumor xenografts and PBS injection; siRNA+PBS, tumor xenografts with silencing Ezrin and PBS injection; Con.+EGF, tumor xenografts and EGF injection; siRNA+EGF, tumor xenografts with silencing Ezrin and EGF injection; * compared to Con.+PBS, P

    Article Snippet: When the mice developed palpable xenografts (more than 0.5 cm diameter), we injected the tumor with phosphate-buffered solution (PBS) (Beyotime) or EGF (0.02 mg/kg) twice a week for 5 weeks.

    Techniques: Injection, Mouse Assay

    Chemical structures and characterizations of untargeted and targeted SHK nanoformulations. Morphologies and R h s of (A) NG/SHK and (B) STP-NG/SHK. (C) Release profiles of SHK from NG/SHK and STP-NG/SHK in PBS without or with 10.0 mM GSH.

    Journal: Theranostics

    Article Title: Sarcoma-Targeting Peptide-Decorated Polypeptide Nanogel Intracellularly Delivers Shikonin for Upregulated Osteosarcoma Necroptosis and Diminished Pulmonary Metastasis

    doi: 10.7150/thno.18299

    Figure Lengend Snippet: Chemical structures and characterizations of untargeted and targeted SHK nanoformulations. Morphologies and R h s of (A) NG/SHK and (B) STP-NG/SHK. (C) Release profiles of SHK from NG/SHK and STP-NG/SHK in PBS without or with 10.0 mM GSH.

    Article Snippet: 143B cells were treated with 2.0 μΜ SHK, NG/SHK, or STP-NG/SHK for 24 h. Cells were washed twice with PBS solution and lysed with radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime Institute of Biotechnology, Beijing, P. R. China) and protease inhibitor (Thermo Scientific, Waltham, USA).

    Techniques:

    Chemical structures and characterizations of untargeted and targeted SHK nanoformulations. Morphologies and R h s of (A) NG/SHK and (B) STP-NG/SHK. (C) Release profiles of SHK from NG/SHK and STP-NG/SHK in PBS without or with 10.0 mM GSH.

    Journal: Theranostics

    Article Title: Sarcoma-Targeting Peptide-Decorated Polypeptide Nanogel Intracellularly Delivers Shikonin for Upregulated Osteosarcoma Necroptosis and Diminished Pulmonary Metastasis

    doi: 10.7150/thno.18299

    Figure Lengend Snippet: Chemical structures and characterizations of untargeted and targeted SHK nanoformulations. Morphologies and R h s of (A) NG/SHK and (B) STP-NG/SHK. (C) Release profiles of SHK from NG/SHK and STP-NG/SHK in PBS without or with 10.0 mM GSH.

    Article Snippet: Western Blot Analyses 143B cells were treated with 2.0 μΜ SHK, NG/SHK, or STP-NG/SHK for 24 h. Cells were washed twice with PBS solution and lysed with radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime Institute of Biotechnology, Beijing, P. R. China) and protease inhibitor (Thermo Scientific, Waltham, USA).

    Techniques: