phosphate buffered solution pbs  (ATCC)


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    ATCC phosphate buffered solution pbs
    Phosphate Buffered Solution Pbs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phosphate buffer solution pbs  (ATCC)


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    ATCC phosphate buffer solution pbs
    The characteristics of Sham control and 5/6 nephrectomized (5/6 Nx) mice <t>with</t> <t>phosphate</t> buffer solution <t>(PBS)</t> or Candida administration ( Candida ) at 20 weeks (wk) after right nephrectomy (20 week post Nx) (see method), as indicated by body weight ( A ), mean arterial pressure ( B ), hematocrit ( C ), renal function (24 h urine protein and serum creatinine) ( D , E ), serum gut-derived uremic toxins, including trimethylamine N-oxide (TMAO) and indoxyl sulfate ( F , G ), liver enzyme (alanine transaminase) ( H ), and serum cytokines (TNF-α, IL-6, and IL-10) ( I – K ) are demonstrated ( n = 11/group).
    Phosphate Buffer Solution Pbs, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Candida Administration in 5/6 Nephrectomized Mice Enhanced Fibrosis in Internal Organs: An Impact of Lipopolysaccharide and (1→3)-β-D-Glucan from Leaky Gut"

    Article Title: Candida Administration in 5/6 Nephrectomized Mice Enhanced Fibrosis in Internal Organs: An Impact of Lipopolysaccharide and (1→3)-β-D-Glucan from Leaky Gut

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms232415987

    The characteristics of Sham control and 5/6 nephrectomized (5/6 Nx) mice with phosphate buffer solution (PBS) or Candida administration ( Candida ) at 20 weeks (wk) after right nephrectomy (20 week post Nx) (see method), as indicated by body weight ( A ), mean arterial pressure ( B ), hematocrit ( C ), renal function (24 h urine protein and serum creatinine) ( D , E ), serum gut-derived uremic toxins, including trimethylamine N-oxide (TMAO) and indoxyl sulfate ( F , G ), liver enzyme (alanine transaminase) ( H ), and serum cytokines (TNF-α, IL-6, and IL-10) ( I – K ) are demonstrated ( n = 11/group).
    Figure Legend Snippet: The characteristics of Sham control and 5/6 nephrectomized (5/6 Nx) mice with phosphate buffer solution (PBS) or Candida administration ( Candida ) at 20 weeks (wk) after right nephrectomy (20 week post Nx) (see method), as indicated by body weight ( A ), mean arterial pressure ( B ), hematocrit ( C ), renal function (24 h urine protein and serum creatinine) ( D , E ), serum gut-derived uremic toxins, including trimethylamine N-oxide (TMAO) and indoxyl sulfate ( F , G ), liver enzyme (alanine transaminase) ( H ), and serum cytokines (TNF-α, IL-6, and IL-10) ( I – K ) are demonstrated ( n = 11/group).

    Techniques Used: Derivative Assay

    The characteristics of Sham control and 5/6 nephrectomized (5/6 Nx) mice with phosphate buffer solution (PBS) or Candida administration ( Candida ) at 20 weeks (wk) after right nephrectomy (20 week post Nx) (see method), as indicated by the gut barrier defect; FITC-dextran assay, endotoxemia, and serum (1→3)-β-D-glucan (BG) ( A – C ), area of organ fibrosis with the gene expression of Toll-like receptor 4 (TLR-4) and Dectin-1 in the kidney ( D – F ), liver ( G – I ), and heart ( J – L ) are demonstrated ( n = 11/group).
    Figure Legend Snippet: The characteristics of Sham control and 5/6 nephrectomized (5/6 Nx) mice with phosphate buffer solution (PBS) or Candida administration ( Candida ) at 20 weeks (wk) after right nephrectomy (20 week post Nx) (see method), as indicated by the gut barrier defect; FITC-dextran assay, endotoxemia, and serum (1→3)-β-D-glucan (BG) ( A – C ), area of organ fibrosis with the gene expression of Toll-like receptor 4 (TLR-4) and Dectin-1 in the kidney ( D – F ), liver ( G – I ), and heart ( J – L ) are demonstrated ( n = 11/group).

    Techniques Used: Expressing

    The representative kidney histopathological pictures stained by hematoxylin and eosin (H&E) ( upper part ) or Masson’s trichrome color ( lower part ) (original magnification 40–200×) of Sham control, 5/6 nephrectomized mice with phosphate buffer solution (5/6 Nx PBS) or Candida administration (5/6 Nx Candida ) are demonstrated.
    Figure Legend Snippet: The representative kidney histopathological pictures stained by hematoxylin and eosin (H&E) ( upper part ) or Masson’s trichrome color ( lower part ) (original magnification 40–200×) of Sham control, 5/6 nephrectomized mice with phosphate buffer solution (5/6 Nx PBS) or Candida administration (5/6 Nx Candida ) are demonstrated.

    Techniques Used: Staining

    The representative liver histopathological pictures stained by hematoxylin and eosin (H&E) ( upper part ) or Masson’s trichrome color ( lower part ) (original magnification 40–200×) of Sham control, 5/6 nephrectomized mice with phosphate buffer solution (5/6 Nx PBS) or Candida administration (5/6 Nx Candida ) are demonstrated.
    Figure Legend Snippet: The representative liver histopathological pictures stained by hematoxylin and eosin (H&E) ( upper part ) or Masson’s trichrome color ( lower part ) (original magnification 40–200×) of Sham control, 5/6 nephrectomized mice with phosphate buffer solution (5/6 Nx PBS) or Candida administration (5/6 Nx Candida ) are demonstrated.

    Techniques Used: Staining

    The representative heart histopathological pictures stained by hematoxylin and eosin (H&E) ( upper part ) or Masson’s trichrome color ( lower part ) (original magnification 40–200×) of Sham control, 5/6 nephrectomized mice with phosphate buffer solution (5/6 Nx PBS) or Candida administration (5/6 Nx Candida ) are demonstrated.
    Figure Legend Snippet: The representative heart histopathological pictures stained by hematoxylin and eosin (H&E) ( upper part ) or Masson’s trichrome color ( lower part ) (original magnification 40–200×) of Sham control, 5/6 nephrectomized mice with phosphate buffer solution (5/6 Nx PBS) or Candida administration (5/6 Nx Candida ) are demonstrated.

    Techniques Used: Staining

    phosphate buffered solution pbs  (ATCC)


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    ATCC phosphate buffered solution pbs
    Phosphate Buffered Solution Pbs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phosphate buffer solution pbs  (ATCC)


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    ATCC phosphate buffer solution pbs
    Phosphate Buffer Solution Pbs, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pbs phosphate buffer solution  (ATCC)


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    ATCC pbs phosphate buffer solution
    Pbs Phosphate Buffer Solution, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phosphate buffer solution pbs  (ATCC)


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    ATCC phosphate buffer solution pbs
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    phosphate buffer solution pbs  (ATCC)


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    ATCC phosphate buffer solution pbs
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    phosphate buffer solution pbs  (ATCC)


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    ATCC phosphate buffer solution pbs
    The translocation of bacteria-free DNA from the gut into the blood circulation in a non-surgical intestinal tight-junction injury model using dextran sulfate solution (DSS)-induced mucositis. Characteristics of mice with DSS or regular drinking water (Water) with oral gavage by bacterial lysate <t>or</t> <t>phosphate</t> buffer solution <t>(PBS)</t> started on days 5–7 of the experiments, as indicated by serum bacteria-free DNA ( A ), gut-barrier defect (FITC-dextran) ( B ), serum lipopolysaccharide (LPS) ( C ), serum pro-inflammatory cytokines (TNF-α and IL-6) ( D , E ) and colon injury score with representative histological pictures on hematoxylin and eosin (H&E) staining ( F , G ) are demonstrated ( n = 6–8/group). The colon pathology of control PBS gavage with regular drinking water (PBS–water) is not demonstrated due to the similarity with control bacterial lysate gavage with water (lysate–water). Arrows indicate inflammatory cell infiltration in DSS-induced intestinal injury. *, p < 0.05 between the indicated groups; the difference between groups was determined by one-way ANOVA with Tukey’s analysis.
    Phosphate Buffer Solution Pbs, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Blood Bacteria-Free DNA in Septic Mice Enhances LPS-Induced Inflammation in Mice through Macrophage Response"

    Article Title: Blood Bacteria-Free DNA in Septic Mice Enhances LPS-Induced Inflammation in Mice through Macrophage Response

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms23031907

    The translocation of bacteria-free DNA from the gut into the blood circulation in a non-surgical intestinal tight-junction injury model using dextran sulfate solution (DSS)-induced mucositis. Characteristics of mice with DSS or regular drinking water (Water) with oral gavage by bacterial lysate or phosphate buffer solution (PBS) started on days 5–7 of the experiments, as indicated by serum bacteria-free DNA ( A ), gut-barrier defect (FITC-dextran) ( B ), serum lipopolysaccharide (LPS) ( C ), serum pro-inflammatory cytokines (TNF-α and IL-6) ( D , E ) and colon injury score with representative histological pictures on hematoxylin and eosin (H&E) staining ( F , G ) are demonstrated ( n = 6–8/group). The colon pathology of control PBS gavage with regular drinking water (PBS–water) is not demonstrated due to the similarity with control bacterial lysate gavage with water (lysate–water). Arrows indicate inflammatory cell infiltration in DSS-induced intestinal injury. *, p < 0.05 between the indicated groups; the difference between groups was determined by one-way ANOVA with Tukey’s analysis.
    Figure Legend Snippet: The translocation of bacteria-free DNA from the gut into the blood circulation in a non-surgical intestinal tight-junction injury model using dextran sulfate solution (DSS)-induced mucositis. Characteristics of mice with DSS or regular drinking water (Water) with oral gavage by bacterial lysate or phosphate buffer solution (PBS) started on days 5–7 of the experiments, as indicated by serum bacteria-free DNA ( A ), gut-barrier defect (FITC-dextran) ( B ), serum lipopolysaccharide (LPS) ( C ), serum pro-inflammatory cytokines (TNF-α and IL-6) ( D , E ) and colon injury score with representative histological pictures on hematoxylin and eosin (H&E) staining ( F , G ) are demonstrated ( n = 6–8/group). The colon pathology of control PBS gavage with regular drinking water (PBS–water) is not demonstrated due to the similarity with control bacterial lysate gavage with water (lysate–water). Arrows indicate inflammatory cell infiltration in DSS-induced intestinal injury. *, p < 0.05 between the indicated groups; the difference between groups was determined by one-way ANOVA with Tukey’s analysis.

    Techniques Used: Translocation Assay, Staining

    sterile phosphate buffered saline solution pbs  (ATCC)


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    ATCC sterile phosphate buffered saline solution pbs
    Sterile Phosphate Buffered Saline Solution Pbs, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phosphate buffer pb solution  (ATCC)


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    ATCC phosphate buffer pb solution
    Phosphate Buffer Pb Solution, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phosphate buffer solution pbs  (ATCC)


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    ATCC phosphate buffer solution pbs
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    ATCC phosphate buffered solution pbs
    Phosphate Buffered Solution Pbs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC phosphate buffer solution pbs
    The characteristics of Sham control and 5/6 nephrectomized (5/6 Nx) mice <t>with</t> <t>phosphate</t> buffer solution <t>(PBS)</t> or Candida administration ( Candida ) at 20 weeks (wk) after right nephrectomy (20 week post Nx) (see method), as indicated by body weight ( A ), mean arterial pressure ( B ), hematocrit ( C ), renal function (24 h urine protein and serum creatinine) ( D , E ), serum gut-derived uremic toxins, including trimethylamine N-oxide (TMAO) and indoxyl sulfate ( F , G ), liver enzyme (alanine transaminase) ( H ), and serum cytokines (TNF-α, IL-6, and IL-10) ( I – K ) are demonstrated ( n = 11/group).
    Phosphate Buffer Solution Pbs, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC pbs phosphate buffer solution
    The characteristics of Sham control and 5/6 nephrectomized (5/6 Nx) mice <t>with</t> <t>phosphate</t> buffer solution <t>(PBS)</t> or Candida administration ( Candida ) at 20 weeks (wk) after right nephrectomy (20 week post Nx) (see method), as indicated by body weight ( A ), mean arterial pressure ( B ), hematocrit ( C ), renal function (24 h urine protein and serum creatinine) ( D , E ), serum gut-derived uremic toxins, including trimethylamine N-oxide (TMAO) and indoxyl sulfate ( F , G ), liver enzyme (alanine transaminase) ( H ), and serum cytokines (TNF-α, IL-6, and IL-10) ( I – K ) are demonstrated ( n = 11/group).
    Pbs Phosphate Buffer Solution, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC sterile phosphate buffered saline solution pbs
    The characteristics of Sham control and 5/6 nephrectomized (5/6 Nx) mice <t>with</t> <t>phosphate</t> buffer solution <t>(PBS)</t> or Candida administration ( Candida ) at 20 weeks (wk) after right nephrectomy (20 week post Nx) (see method), as indicated by body weight ( A ), mean arterial pressure ( B ), hematocrit ( C ), renal function (24 h urine protein and serum creatinine) ( D , E ), serum gut-derived uremic toxins, including trimethylamine N-oxide (TMAO) and indoxyl sulfate ( F , G ), liver enzyme (alanine transaminase) ( H ), and serum cytokines (TNF-α, IL-6, and IL-10) ( I – K ) are demonstrated ( n = 11/group).
    Sterile Phosphate Buffered Saline Solution Pbs, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC phosphate buffer pb solution
    The characteristics of Sham control and 5/6 nephrectomized (5/6 Nx) mice <t>with</t> <t>phosphate</t> buffer solution <t>(PBS)</t> or Candida administration ( Candida ) at 20 weeks (wk) after right nephrectomy (20 week post Nx) (see method), as indicated by body weight ( A ), mean arterial pressure ( B ), hematocrit ( C ), renal function (24 h urine protein and serum creatinine) ( D , E ), serum gut-derived uremic toxins, including trimethylamine N-oxide (TMAO) and indoxyl sulfate ( F , G ), liver enzyme (alanine transaminase) ( H ), and serum cytokines (TNF-α, IL-6, and IL-10) ( I – K ) are demonstrated ( n = 11/group).
    Phosphate Buffer Pb Solution, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The characteristics of Sham control and 5/6 nephrectomized (5/6 Nx) mice with phosphate buffer solution (PBS) or Candida administration ( Candida ) at 20 weeks (wk) after right nephrectomy (20 week post Nx) (see method), as indicated by body weight ( A ), mean arterial pressure ( B ), hematocrit ( C ), renal function (24 h urine protein and serum creatinine) ( D , E ), serum gut-derived uremic toxins, including trimethylamine N-oxide (TMAO) and indoxyl sulfate ( F , G ), liver enzyme (alanine transaminase) ( H ), and serum cytokines (TNF-α, IL-6, and IL-10) ( I – K ) are demonstrated ( n = 11/group).

    Journal: International Journal of Molecular Sciences

    Article Title: Candida Administration in 5/6 Nephrectomized Mice Enhanced Fibrosis in Internal Organs: An Impact of Lipopolysaccharide and (1→3)-β-D-Glucan from Leaky Gut

    doi: 10.3390/ijms232415987

    Figure Lengend Snippet: The characteristics of Sham control and 5/6 nephrectomized (5/6 Nx) mice with phosphate buffer solution (PBS) or Candida administration ( Candida ) at 20 weeks (wk) after right nephrectomy (20 week post Nx) (see method), as indicated by body weight ( A ), mean arterial pressure ( B ), hematocrit ( C ), renal function (24 h urine protein and serum creatinine) ( D , E ), serum gut-derived uremic toxins, including trimethylamine N-oxide (TMAO) and indoxyl sulfate ( F , G ), liver enzyme (alanine transaminase) ( H ), and serum cytokines (TNF-α, IL-6, and IL-10) ( I – K ) are demonstrated ( n = 11/group).

    Article Snippet: After that, the 5/6 Nx mice were orally administered phosphate buffer solution (PBS) (5/6 Nx PBS) or Candida albicans (5/6 Nx + Candida ) to explore the impact of gut fungi using C. albicans from the American Type Culture Collection (ATCC 90028) (Fisher Scientific, Waltham, MA, USA), which was cultured overnight on Sabouraud dextrose broth (SDB) (Oxoid, Hampshire, UK) at 35 °C for 48 h before enumeration using a hemocytometer.

    Techniques: Derivative Assay

    The characteristics of Sham control and 5/6 nephrectomized (5/6 Nx) mice with phosphate buffer solution (PBS) or Candida administration ( Candida ) at 20 weeks (wk) after right nephrectomy (20 week post Nx) (see method), as indicated by the gut barrier defect; FITC-dextran assay, endotoxemia, and serum (1→3)-β-D-glucan (BG) ( A – C ), area of organ fibrosis with the gene expression of Toll-like receptor 4 (TLR-4) and Dectin-1 in the kidney ( D – F ), liver ( G – I ), and heart ( J – L ) are demonstrated ( n = 11/group).

    Journal: International Journal of Molecular Sciences

    Article Title: Candida Administration in 5/6 Nephrectomized Mice Enhanced Fibrosis in Internal Organs: An Impact of Lipopolysaccharide and (1→3)-β-D-Glucan from Leaky Gut

    doi: 10.3390/ijms232415987

    Figure Lengend Snippet: The characteristics of Sham control and 5/6 nephrectomized (5/6 Nx) mice with phosphate buffer solution (PBS) or Candida administration ( Candida ) at 20 weeks (wk) after right nephrectomy (20 week post Nx) (see method), as indicated by the gut barrier defect; FITC-dextran assay, endotoxemia, and serum (1→3)-β-D-glucan (BG) ( A – C ), area of organ fibrosis with the gene expression of Toll-like receptor 4 (TLR-4) and Dectin-1 in the kidney ( D – F ), liver ( G – I ), and heart ( J – L ) are demonstrated ( n = 11/group).

    Article Snippet: After that, the 5/6 Nx mice were orally administered phosphate buffer solution (PBS) (5/6 Nx PBS) or Candida albicans (5/6 Nx + Candida ) to explore the impact of gut fungi using C. albicans from the American Type Culture Collection (ATCC 90028) (Fisher Scientific, Waltham, MA, USA), which was cultured overnight on Sabouraud dextrose broth (SDB) (Oxoid, Hampshire, UK) at 35 °C for 48 h before enumeration using a hemocytometer.

    Techniques: Expressing

    The representative kidney histopathological pictures stained by hematoxylin and eosin (H&E) ( upper part ) or Masson’s trichrome color ( lower part ) (original magnification 40–200×) of Sham control, 5/6 nephrectomized mice with phosphate buffer solution (5/6 Nx PBS) or Candida administration (5/6 Nx Candida ) are demonstrated.

    Journal: International Journal of Molecular Sciences

    Article Title: Candida Administration in 5/6 Nephrectomized Mice Enhanced Fibrosis in Internal Organs: An Impact of Lipopolysaccharide and (1→3)-β-D-Glucan from Leaky Gut

    doi: 10.3390/ijms232415987

    Figure Lengend Snippet: The representative kidney histopathological pictures stained by hematoxylin and eosin (H&E) ( upper part ) or Masson’s trichrome color ( lower part ) (original magnification 40–200×) of Sham control, 5/6 nephrectomized mice with phosphate buffer solution (5/6 Nx PBS) or Candida administration (5/6 Nx Candida ) are demonstrated.

    Article Snippet: After that, the 5/6 Nx mice were orally administered phosphate buffer solution (PBS) (5/6 Nx PBS) or Candida albicans (5/6 Nx + Candida ) to explore the impact of gut fungi using C. albicans from the American Type Culture Collection (ATCC 90028) (Fisher Scientific, Waltham, MA, USA), which was cultured overnight on Sabouraud dextrose broth (SDB) (Oxoid, Hampshire, UK) at 35 °C for 48 h before enumeration using a hemocytometer.

    Techniques: Staining

    The representative liver histopathological pictures stained by hematoxylin and eosin (H&E) ( upper part ) or Masson’s trichrome color ( lower part ) (original magnification 40–200×) of Sham control, 5/6 nephrectomized mice with phosphate buffer solution (5/6 Nx PBS) or Candida administration (5/6 Nx Candida ) are demonstrated.

    Journal: International Journal of Molecular Sciences

    Article Title: Candida Administration in 5/6 Nephrectomized Mice Enhanced Fibrosis in Internal Organs: An Impact of Lipopolysaccharide and (1→3)-β-D-Glucan from Leaky Gut

    doi: 10.3390/ijms232415987

    Figure Lengend Snippet: The representative liver histopathological pictures stained by hematoxylin and eosin (H&E) ( upper part ) or Masson’s trichrome color ( lower part ) (original magnification 40–200×) of Sham control, 5/6 nephrectomized mice with phosphate buffer solution (5/6 Nx PBS) or Candida administration (5/6 Nx Candida ) are demonstrated.

    Article Snippet: After that, the 5/6 Nx mice were orally administered phosphate buffer solution (PBS) (5/6 Nx PBS) or Candida albicans (5/6 Nx + Candida ) to explore the impact of gut fungi using C. albicans from the American Type Culture Collection (ATCC 90028) (Fisher Scientific, Waltham, MA, USA), which was cultured overnight on Sabouraud dextrose broth (SDB) (Oxoid, Hampshire, UK) at 35 °C for 48 h before enumeration using a hemocytometer.

    Techniques: Staining

    The representative heart histopathological pictures stained by hematoxylin and eosin (H&E) ( upper part ) or Masson’s trichrome color ( lower part ) (original magnification 40–200×) of Sham control, 5/6 nephrectomized mice with phosphate buffer solution (5/6 Nx PBS) or Candida administration (5/6 Nx Candida ) are demonstrated.

    Journal: International Journal of Molecular Sciences

    Article Title: Candida Administration in 5/6 Nephrectomized Mice Enhanced Fibrosis in Internal Organs: An Impact of Lipopolysaccharide and (1→3)-β-D-Glucan from Leaky Gut

    doi: 10.3390/ijms232415987

    Figure Lengend Snippet: The representative heart histopathological pictures stained by hematoxylin and eosin (H&E) ( upper part ) or Masson’s trichrome color ( lower part ) (original magnification 40–200×) of Sham control, 5/6 nephrectomized mice with phosphate buffer solution (5/6 Nx PBS) or Candida administration (5/6 Nx Candida ) are demonstrated.

    Article Snippet: After that, the 5/6 Nx mice were orally administered phosphate buffer solution (PBS) (5/6 Nx PBS) or Candida albicans (5/6 Nx + Candida ) to explore the impact of gut fungi using C. albicans from the American Type Culture Collection (ATCC 90028) (Fisher Scientific, Waltham, MA, USA), which was cultured overnight on Sabouraud dextrose broth (SDB) (Oxoid, Hampshire, UK) at 35 °C for 48 h before enumeration using a hemocytometer.

    Techniques: Staining