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bio-rad phosphate buffered saline
Phosphate Buffered Saline, supplied by bio-rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphate buffered saline - by Bioz Stars, 2021-03
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Related Articles

DC Protein Assay:

Article Title: Confounding factors of ultrafiltration and protein analysis in extracellular vesicle research
Article Snippet: .. A dilution series of 0 to 10% of Optiprep in PBS was obtained as a standard curve for performing absorbance readings with DC Protein assay (Bio-Rad, Hercules, California, USA), according to manufacturer’s instructions. ..

Protein Concentration:

Article Title: Human semen contains exosomes with potent anti-HIV-1 activity
Article Snippet: After 16 h of ultracentrifugation at 100,000 × g (SW41Ti rotor, Beckman Coulter), exosomes were collected from the main band at the 2 M/L and 0.25 M/L interface, resuspended in PBS and pelleted by ultracentrifugation for 30 min at 100,000 × g . .. The exosome pellets were resuspended in a volume of sterile PBS equivalent to the original volume of undiluted seminal plasma, aliquoted, protein concentration quantified by the Bradford method (Bio-Rad), and stored at −80°C until use. .. We determined that the method of purification did not alter the ability of SE to inhibit HIV replication.

other:

Article Title: A novel gonorrhea vaccine composed of MetQ lipoprotein formulated with CpG shortens experimental murine infection
Article Snippet: Round bottom high-binding 96 well microtiter plates (Nunc) were coated with 0.5 μg of rMetQ suspended in phosphate buffered saline (PBS) and were blocked with PBS supplemented with 3% Bovine Serum Albumin.

Article Title: A Newcastle disease virus (NDV) expressing membrane-anchored spike as a cost-effective inactivated SARS-CoV-2 vaccine
Article Snippet: The pelleted virus was re-suspended in 300 μL phosphate buffered saline (PBS) and stored at −80°C.

Article Title: Development of Novel High-Resolution Size-Guided Turbidimetry-Enabled Particle Purification Liquid Chromatography (PPLC): Extracellular Vesicles and Membraneless Condensates in Focus
Article Snippet: The 1× or 0.1× phosphate-buffered saline (PBS) was used as the mobile phase.

Incubation:

Article Title: The development and use of an ELISA-based method to follow the distribution of cellulase monocomponents during the hydrolysis of pretreated corn stover
Article Snippet: After incubation with each of the enzymes, the plate was washed, and the PAb, diluted in PBS with 1% (w/v) BSA, was added to each well. .. Following another washing step, the third antibody, a commercial GAR-AP (Bio-Rad) diluted in PBS with 1% (w/v) BSA, was added to the wells and incubated for another hour. .. After a final washing step, 35 mg/ml of p -nitrophenylphosphate (Bio-Rad), a substrate for alkaline phosphatase (AP), was added to the wells and the plate was incubated at room temperature for 30 minutes or until sufficient colour had developed.

Infection:

Article Title: Low doses of bioherbicide favour prion aggregation and propagation in vivo
Article Snippet: Then, fluorescence experiments were conducted as described previously by excitation of the A6 compound at 372 nm. .. Brains from a terminally sick mouse (infected with 22L prions) or from a non-infected mouse were homogenized in 10% (w/v) sterile PBS solution using microbead-containing tubes and a Ribolysor apparatus (Biorad, Marnes la Coquette, France). ..

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  • 86
    Bio-Rad pbs solution
    a SDS-PAGE of blank LN homogenate, free dye, <t>bevacizumab</t> in <t>PBS</t> (2 μg), bevacizumab–800CW conjugate (2 μg) in PBS and bevacizumab–800CW conjugate (2 μg) spiked in blank mouse lymph node
    Pbs Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs solution/product/Bio-Rad
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    86/100 stars
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    86
    Bio-Rad biotinylated goat anti swine igg
    PRRSV-specific <t>IgG</t> detection via immunoperoxidase monolayer assay (IPMA) and immunofluorescence assay (IFA). PRRSV-infected cells were incubated with 1:2 dilutions of oral fluid samples. PRRSV-specific IgG antibodies bound to infected cells were subsequently detected via IPMA (A) , using <t>biotinylated</t> goat-anti-swine IgG antibodies and horseradish peroxidase-conjugated streptavidine, or IFA (B) , using goat-anti-swine IgG antibodies and FITC-conjugated anti-goat antibodies.
    Biotinylated Goat Anti Swine Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti swine igg/product/Bio-Rad
    Average 86 stars, based on 1 article reviews
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    biotinylated goat anti swine igg - by Bioz Stars, 2021-03
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    99
    Bio-Rad bovine serum albumin pbs
    Histopathological changes shown by H E staining in the lung, heart and brain at 6 dpi. For each virus, selected tissues from mice in the virus-infected group (A, C, E, H, J, L, O, Q, S) , the <t>mAb</t> D7 treated group (B, D, F, I, K, M, P, R, T) and the <t>PBS</t> group (G, N, U) are shown, respectively. Short arrows indicate the lesions. All the images are shown at 100 × magnification.
    Bovine Serum Albumin Pbs, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bovine serum albumin pbs/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
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    85
    Bio-Rad mouse anti human er β
    ER- α , ER- β  and SRC-1 protein expression in paraffin-embedded invasive breast carcinoma and normal breast tissue specimens. ( A ) Immunohistochemical localisation of ER- α , ER- β  and SRC-1 (× 200) in primary breast cancer counterstained with haematoxylin and matched IgG controls. ( B ) ER- α , ER- β  and SRC-1, (× 200) protein expression in normal breast tissue. ( C ) Immunofluorescent colocalisation of ER- α  with SRC-1 (× 200) and ER- β  with SRC-1 (× 200) invasive breast carcinoma.
    Mouse Anti Human Er β, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human er β/product/Bio-Rad
    Average 85 stars, based on 1 article reviews
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    Image Search Results


    a SDS-PAGE of blank LN homogenate, free dye, bevacizumab in PBS (2 μg), bevacizumab–800CW conjugate (2 μg) in PBS and bevacizumab–800CW conjugate (2 μg) spiked in blank mouse lymph node

    Journal: The AAPS Journal

    Article Title: Pharmacokinetics, Lymph Node Uptake, and Mechanistic PK Model of Near-Infrared Dye-Labeled Bevacizumab After IV and SC Administration in Mice

    doi: 10.1208/s12248-012-9342-9

    Figure Lengend Snippet: a SDS-PAGE of blank LN homogenate, free dye, bevacizumab in PBS (2 μg), bevacizumab–800CW conjugate (2 μg) in PBS and bevacizumab–800CW conjugate (2 μg) spiked in blank mouse lymph node

    Article Snippet: Briefly, 8 μL of 500 μg/mL of bevacizumab IRDye 800CW conjugate or 500 μg/mL of bevacizumab in PBS solution was mixed with 12 μL of sample buffer (Bio-Rad, 2% SDS w/v , 25% glycerol v/v , 0.001% bromophenol blue w/v , 62.5 mM Tris–HCl, pH 6.8) with 5% β-mercaptoethanol, and 10 μL of each mixture was loaded and separated on a 10% polyacrylamide gel.

    Techniques: SDS Page

    PRRSV-specific IgG detection via immunoperoxidase monolayer assay (IPMA) and immunofluorescence assay (IFA). PRRSV-infected cells were incubated with 1:2 dilutions of oral fluid samples. PRRSV-specific IgG antibodies bound to infected cells were subsequently detected via IPMA (A) , using biotinylated goat-anti-swine IgG antibodies and horseradish peroxidase-conjugated streptavidine, or IFA (B) , using goat-anti-swine IgG antibodies and FITC-conjugated anti-goat antibodies.

    Journal: BMC Veterinary Research

    Article Title: Detection of total and PRRSV-specific antibodies in oral fluids collected with different rope types from PRRSV-vaccinated and experimentally infected pigs

    doi: 10.1186/1746-6148-10-134

    Figure Lengend Snippet: PRRSV-specific IgG detection via immunoperoxidase monolayer assay (IPMA) and immunofluorescence assay (IFA). PRRSV-infected cells were incubated with 1:2 dilutions of oral fluid samples. PRRSV-specific IgG antibodies bound to infected cells were subsequently detected via IPMA (A) , using biotinylated goat-anti-swine IgG antibodies and horseradish peroxidase-conjugated streptavidine, or IFA (B) , using goat-anti-swine IgG antibodies and FITC-conjugated anti-goat antibodies.

    Article Snippet: Subsequently, cells were incubated with biotinylated goat- anti-swine IgA (AbD Serotec; 1:10 in PBS) or biotinylated goat- anti-swine IgG (AbD Serotec; 1:10 in PBS) for 1 h at 37°C.

    Techniques: Immunofluorescence, Infection, Incubation

    Histopathological changes shown by H E staining in the lung, heart and brain at 6 dpi. For each virus, selected tissues from mice in the virus-infected group (A, C, E, H, J, L, O, Q, S) , the mAb D7 treated group (B, D, F, I, K, M, P, R, T) and the PBS group (G, N, U) are shown, respectively. Short arrows indicate the lesions. All the images are shown at 100 × magnification.

    Journal: Veterinary Research

    Article Title: Monoclonal antibody specific to HA2 glycopeptide protects mice from H3N2 influenza virus infection

    doi: 10.1186/s13567-015-0146-7

    Figure Lengend Snippet: Histopathological changes shown by H E staining in the lung, heart and brain at 6 dpi. For each virus, selected tissues from mice in the virus-infected group (A, C, E, H, J, L, O, Q, S) , the mAb D7 treated group (B, D, F, I, K, M, P, R, T) and the PBS group (G, N, U) are shown, respectively. Short arrows indicate the lesions. All the images are shown at 100 × magnification.

    Article Snippet: In brief, sections were blocked with 1% bovine serum albumin/PBS, stained with mAb D7 at a dilution of 1:5000 for one hour at 37 °C, followed by biotin conjugated goat anti-mouse immunoglobulin (Bio-Rad) at a dilution of 1:200 for 30 min at 37 °C.

    Techniques: Staining, Mouse Assay, Infection

    Body weight changes and viral loads in the lungs of mice treated with mAb D7. Three groups of 6-week-old BALB/c mice were challenged with approximately 10 7 TCID 50 of strains JS/10 (A, D) , GD/12 (B, E) and SD/05 (C, F) , respectively. In each virus group, mice were pretreated with 20 mg/kg of mAb D7, mAb IgG or PBS 1 day before viral challenge. Mice were monitored for body weight loss throughout the 14-day observation period. For body weight change (A, B, C) , the results are expressed in terms of percent body weight. * P

    Journal: Veterinary Research

    Article Title: Monoclonal antibody specific to HA2 glycopeptide protects mice from H3N2 influenza virus infection

    doi: 10.1186/s13567-015-0146-7

    Figure Lengend Snippet: Body weight changes and viral loads in the lungs of mice treated with mAb D7. Three groups of 6-week-old BALB/c mice were challenged with approximately 10 7 TCID 50 of strains JS/10 (A, D) , GD/12 (B, E) and SD/05 (C, F) , respectively. In each virus group, mice were pretreated with 20 mg/kg of mAb D7, mAb IgG or PBS 1 day before viral challenge. Mice were monitored for body weight loss throughout the 14-day observation period. For body weight change (A, B, C) , the results are expressed in terms of percent body weight. * P

    Article Snippet: In brief, sections were blocked with 1% bovine serum albumin/PBS, stained with mAb D7 at a dilution of 1:5000 for one hour at 37 °C, followed by biotin conjugated goat anti-mouse immunoglobulin (Bio-Rad) at a dilution of 1:200 for 30 min at 37 °C.

    Techniques: Mouse Assay

    Characterization of IFN-γ and TNF-α secretion from lung tissues of mice challenged with virus. Cytokine concentrations were measured by ELISA in supernatants of homogenates from the lungs infected with three virus strains. Mice were pretreated with 20 mg/kg of mAb D7, mAb IgG or PBS 1 day before challenged with virus JS/10 (A, D) , GD/12 (B, E) or SD/05 (C, F) , respectively. The cytokine levels were measured in the infected mice on days 2, 4, 6, 10 and 14 post challenged. The results are expressed in terms of pg/mL. * P

    Journal: Veterinary Research

    Article Title: Monoclonal antibody specific to HA2 glycopeptide protects mice from H3N2 influenza virus infection

    doi: 10.1186/s13567-015-0146-7

    Figure Lengend Snippet: Characterization of IFN-γ and TNF-α secretion from lung tissues of mice challenged with virus. Cytokine concentrations were measured by ELISA in supernatants of homogenates from the lungs infected with three virus strains. Mice were pretreated with 20 mg/kg of mAb D7, mAb IgG or PBS 1 day before challenged with virus JS/10 (A, D) , GD/12 (B, E) or SD/05 (C, F) , respectively. The cytokine levels were measured in the infected mice on days 2, 4, 6, 10 and 14 post challenged. The results are expressed in terms of pg/mL. * P

    Article Snippet: In brief, sections were blocked with 1% bovine serum albumin/PBS, stained with mAb D7 at a dilution of 1:5000 for one hour at 37 °C, followed by biotin conjugated goat anti-mouse immunoglobulin (Bio-Rad) at a dilution of 1:200 for 30 min at 37 °C.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Infection

    Viral loads in collected tissues and fecal samples of mice at 6 dpi. Mice were pretreated with 20 mg/kg of mAb D7, mAb IgG or PBS 1 day before viral challenge with virus JS/10 (A) , GD/12 (B) or SD/05 (C) , respectively. In each virus group, the lung, heart, brain, spleen, intestine, feces and blood of mice were collected for determination of viral loads using real-time PCR at 6 days post-challenge. * P

    Journal: Veterinary Research

    Article Title: Monoclonal antibody specific to HA2 glycopeptide protects mice from H3N2 influenza virus infection

    doi: 10.1186/s13567-015-0146-7

    Figure Lengend Snippet: Viral loads in collected tissues and fecal samples of mice at 6 dpi. Mice were pretreated with 20 mg/kg of mAb D7, mAb IgG or PBS 1 day before viral challenge with virus JS/10 (A) , GD/12 (B) or SD/05 (C) , respectively. In each virus group, the lung, heart, brain, spleen, intestine, feces and blood of mice were collected for determination of viral loads using real-time PCR at 6 days post-challenge. * P

    Article Snippet: In brief, sections were blocked with 1% bovine serum albumin/PBS, stained with mAb D7 at a dilution of 1:5000 for one hour at 37 °C, followed by biotin conjugated goat anti-mouse immunoglobulin (Bio-Rad) at a dilution of 1:200 for 30 min at 37 °C.

    Techniques: Mouse Assay, Real-time Polymerase Chain Reaction

    Immunohistochemical detection of influenza viral antigen in the lung, heart and brain at 6 dpi. For each virus, selected tissues from mice in the virus-infected group (A, C, E, H, J, L, O, Q, S) , the mAb D7 treated group (B, D, F, I, K, M, P, R, T) and the PBS group (G, N, U) are shown, respectively. All the images are shown at 400 × magnification.

    Journal: Veterinary Research

    Article Title: Monoclonal antibody specific to HA2 glycopeptide protects mice from H3N2 influenza virus infection

    doi: 10.1186/s13567-015-0146-7

    Figure Lengend Snippet: Immunohistochemical detection of influenza viral antigen in the lung, heart and brain at 6 dpi. For each virus, selected tissues from mice in the virus-infected group (A, C, E, H, J, L, O, Q, S) , the mAb D7 treated group (B, D, F, I, K, M, P, R, T) and the PBS group (G, N, U) are shown, respectively. All the images are shown at 400 × magnification.

    Article Snippet: In brief, sections were blocked with 1% bovine serum albumin/PBS, stained with mAb D7 at a dilution of 1:5000 for one hour at 37 °C, followed by biotin conjugated goat anti-mouse immunoglobulin (Bio-Rad) at a dilution of 1:200 for 30 min at 37 °C.

    Techniques: Immunohistochemistry, Mouse Assay, Infection

    ER- α , ER- β  and SRC-1 protein expression in paraffin-embedded invasive breast carcinoma and normal breast tissue specimens. ( A ) Immunohistochemical localisation of ER- α , ER- β  and SRC-1 (× 200) in primary breast cancer counterstained with haematoxylin and matched IgG controls. ( B ) ER- α , ER- β  and SRC-1, (× 200) protein expression in normal breast tissue. ( C ) Immunofluorescent colocalisation of ER- α  with SRC-1 (× 200) and ER- β  with SRC-1 (× 200) invasive breast carcinoma.

    Journal: British Journal of Cancer

    Article Title: Inverse relationship between ER-β and SRC-1 predicts outcome in endocrine-resistant breast cancer

    doi: 10.1038/sj.bjc.6602156

    Figure Lengend Snippet: ER- α , ER- β and SRC-1 protein expression in paraffin-embedded invasive breast carcinoma and normal breast tissue specimens. ( A ) Immunohistochemical localisation of ER- α , ER- β and SRC-1 (× 200) in primary breast cancer counterstained with haematoxylin and matched IgG controls. ( B ) ER- α , ER- β and SRC-1, (× 200) protein expression in normal breast tissue. ( C ) Immunofluorescent colocalisation of ER- α with SRC-1 (× 200) and ER- β with SRC-1 (× 200) invasive breast carcinoma.

    Article Snippet: Sections were incubated with primary antibodies; rabbit anti-human ER-α (1 μ g ml−1 ), goat anti-human SRC-1 (1 μ g ml−1 ) (Santa Cruz, CA, USA) and mouse anti human ER-β (one in 20 in PBS) (Serotec, Oxford, UK) – for 60 min at room temperature.

    Techniques: Expressing, Immunohistochemistry

    Kaplan–Meier estimates of disease-free survival (DFS). ( A ) Disease-free survival according to ER- β  expression and ( B ) according to SRC-1 expression.

    Journal: British Journal of Cancer

    Article Title: Inverse relationship between ER-β and SRC-1 predicts outcome in endocrine-resistant breast cancer

    doi: 10.1038/sj.bjc.6602156

    Figure Lengend Snippet: Kaplan–Meier estimates of disease-free survival (DFS). ( A ) Disease-free survival according to ER- β expression and ( B ) according to SRC-1 expression.

    Article Snippet: Sections were incubated with primary antibodies; rabbit anti-human ER-α (1 μ g ml−1 ), goat anti-human SRC-1 (1 μ g ml−1 ) (Santa Cruz, CA, USA) and mouse anti human ER-β (one in 20 in PBS) (Serotec, Oxford, UK) – for 60 min at room temperature.

    Techniques: Expressing