phosphate buffered saline pbs solution  (Thermo Fisher)


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    Name:
    Phosphate Buffered Saline
    Description:
    Recover microorganisms from multiple sample types including rinse samples from clinical instruments and environmental monitoring samples by creating a pH balanced environment with Thermo Scientific Phosphate Buffered Saline with Poly 80 PBST a buffer solution commonly used in biological research
    Catalog Number:
    r112502
    Price:
    None
    Applications:
    Blood Culture|Clinical|Clinical Microbiology
    Category:
    Cell Culture Transfection Reagents
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    Structured Review

    Thermo Fisher phosphate buffered saline pbs solution
    Inertio-elastic focusing of bioparticles based on deformability and shape ( a ) Deformation statistics of WBCs in <t>PBS,</t> a low-molecular weight (357 <t>kDa)</t> HA solution and a high-molecular weight (1,650 kDa) HA solution. The magnitude of WBC stretching is expressed in terms of aspect ratio AR = a x /a z . Scale bar, 10 µm. The error bars indicate the s.d. in the WBC aspect ratio at each flow rate. ( b ) LEF and particle trajectory analysis images of WBCs in PBS, 357 kDa HA solution and 1,650 kDa HA solution at Q = 13 ml min −1 . ( c ) Particle trajectory analysis (PTA) images of anisotropic PEG particles in 1,650 kDa HA solution at Q = 20 ml min −1 . Dashed red lines indicate channel centerline. Scale bar, 30 µm. Measurements of lateral position z and instantaneous orientation angle θ are plotted for each PEG particle in water (blue) and in the HA solution (green).
    Recover microorganisms from multiple sample types including rinse samples from clinical instruments and environmental monitoring samples by creating a pH balanced environment with Thermo Scientific Phosphate Buffered Saline with Poly 80 PBST a buffer solution commonly used in biological research
    https://www.bioz.com/result/phosphate buffered saline pbs solution/product/Thermo Fisher
    Average 99 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    phosphate buffered saline pbs solution - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Inertio-elastic focusing of bioparticles in microchannels at high throughput"

    Article Title: Inertio-elastic focusing of bioparticles in microchannels at high throughput

    Journal: Nature communications

    doi: 10.1038/ncomms5120

    Inertio-elastic focusing of bioparticles based on deformability and shape ( a ) Deformation statistics of WBCs in PBS, a low-molecular weight (357 kDa) HA solution and a high-molecular weight (1,650 kDa) HA solution. The magnitude of WBC stretching is expressed in terms of aspect ratio AR = a x /a z . Scale bar, 10 µm. The error bars indicate the s.d. in the WBC aspect ratio at each flow rate. ( b ) LEF and particle trajectory analysis images of WBCs in PBS, 357 kDa HA solution and 1,650 kDa HA solution at Q = 13 ml min −1 . ( c ) Particle trajectory analysis (PTA) images of anisotropic PEG particles in 1,650 kDa HA solution at Q = 20 ml min −1 . Dashed red lines indicate channel centerline. Scale bar, 30 µm. Measurements of lateral position z and instantaneous orientation angle θ are plotted for each PEG particle in water (blue) and in the HA solution (green).
    Figure Legend Snippet: Inertio-elastic focusing of bioparticles based on deformability and shape ( a ) Deformation statistics of WBCs in PBS, a low-molecular weight (357 kDa) HA solution and a high-molecular weight (1,650 kDa) HA solution. The magnitude of WBC stretching is expressed in terms of aspect ratio AR = a x /a z . Scale bar, 10 µm. The error bars indicate the s.d. in the WBC aspect ratio at each flow rate. ( b ) LEF and particle trajectory analysis images of WBCs in PBS, 357 kDa HA solution and 1,650 kDa HA solution at Q = 13 ml min −1 . ( c ) Particle trajectory analysis (PTA) images of anisotropic PEG particles in 1,650 kDa HA solution at Q = 20 ml min −1 . Dashed red lines indicate channel centerline. Scale bar, 30 µm. Measurements of lateral position z and instantaneous orientation angle θ are plotted for each PEG particle in water (blue) and in the HA solution (green).

    Techniques Used: Molecular Weight, Flow Cytometry

    2) Product Images from "Periodic and simultaneous quantification of blood viscosity and red blood cell aggregation using a microfluidic platform under in-vitro closed-loop circulation"

    Article Title: Periodic and simultaneous quantification of blood viscosity and red blood cell aggregation using a microfluidic platform under in-vitro closed-loop circulation

    Journal: Biomicrofluidics

    doi: 10.1063/1.5017052

    Schematics for the periodic and simultaneous measurement of blood viscosity RBC aggregation using a microfluidic platform under in-vitro closed-loop circulation. (A) Schematics of the experimental setup including an in-vitro closed-loop circulation and a microfluidic platform. (a) In-vitro closed-loop circulation established by connecting several components (peristaltic pump, ACU, FD, and reservoir) with polyethylene tubes (L 5 , L 6 , L 7 , L 8 , and L 9 ) in series. After the reservoir was filled with blood (10 ml), a peristaltic pump was set to ω = 15 rpm. The air cavity in the ACU was adjusted to 2.5 ml. A specific concentration of dextran solution (i.e., C dextran = 100 mg/ml) was delivered into the reservoir by operating the syringe pump. (b) A microfluidic platform including a microfluidic device (VA), PV, and syringe pump. The outlet of the FD was connected to the inlet (B) of the VA with a polyethylene tube (L 2 ). To control the blood flow from the FD to the VA, a PV was installed in front of the inlet (B). 1× PBS solution was supplied into inlet (A) with a syringe pump. (B) Flow-rate profile of two fluids with a syringe pump (i.e., Q PBS and Q Dextran ) and PV operation (i.e., open and close) for each period. (C) Quantification of image intensity (⟨I⟩) and blood flow-rate (Q μ PIV ). (a) Quantification of the image intensity (⟨I⟩) within the ROI (484 × 150 pixels) in the upper channel. (b) Quantification of blood velocity fields with a time-resolved micro-PIV technique. The blood flow-rate (Q μ PIV ) was quantified by multiplying averaged blood velocity (⟨U⟩) by the cross-sectional area (A c ) (i.e., Q μ PIV = ⟨U⟩·A c ). (c) A simple fluidic circuit with discrete elements including fluidic resistances (R P , R B ), and flow rates (Q PBS , Q Blood ). The interface between two fluids was evaluated by converting the gray image into a binary image. (D) For a preliminary demonstration, blood (Hct = 50%) was prepared by adding normal RBCs to autologous plasma. Here, the period was set to T = 400 s. The inset shows microscopic images at a specific time (t) [(a) t = 10 s, (b) t = 100 s, (c) t = 150 s, (d) t = 200 s, (e) t = 300 s, and (f) t = 400 s]. During each period, at constant blood flow (i.e., 0
    Figure Legend Snippet: Schematics for the periodic and simultaneous measurement of blood viscosity RBC aggregation using a microfluidic platform under in-vitro closed-loop circulation. (A) Schematics of the experimental setup including an in-vitro closed-loop circulation and a microfluidic platform. (a) In-vitro closed-loop circulation established by connecting several components (peristaltic pump, ACU, FD, and reservoir) with polyethylene tubes (L 5 , L 6 , L 7 , L 8 , and L 9 ) in series. After the reservoir was filled with blood (10 ml), a peristaltic pump was set to ω = 15 rpm. The air cavity in the ACU was adjusted to 2.5 ml. A specific concentration of dextran solution (i.e., C dextran = 100 mg/ml) was delivered into the reservoir by operating the syringe pump. (b) A microfluidic platform including a microfluidic device (VA), PV, and syringe pump. The outlet of the FD was connected to the inlet (B) of the VA with a polyethylene tube (L 2 ). To control the blood flow from the FD to the VA, a PV was installed in front of the inlet (B). 1× PBS solution was supplied into inlet (A) with a syringe pump. (B) Flow-rate profile of two fluids with a syringe pump (i.e., Q PBS and Q Dextran ) and PV operation (i.e., open and close) for each period. (C) Quantification of image intensity (⟨I⟩) and blood flow-rate (Q μ PIV ). (a) Quantification of the image intensity (⟨I⟩) within the ROI (484 × 150 pixels) in the upper channel. (b) Quantification of blood velocity fields with a time-resolved micro-PIV technique. The blood flow-rate (Q μ PIV ) was quantified by multiplying averaged blood velocity (⟨U⟩) by the cross-sectional area (A c ) (i.e., Q μ PIV = ⟨U⟩·A c ). (c) A simple fluidic circuit with discrete elements including fluidic resistances (R P , R B ), and flow rates (Q PBS , Q Blood ). The interface between two fluids was evaluated by converting the gray image into a binary image. (D) For a preliminary demonstration, blood (Hct = 50%) was prepared by adding normal RBCs to autologous plasma. Here, the period was set to T = 400 s. The inset shows microscopic images at a specific time (t) [(a) t = 10 s, (b) t = 100 s, (c) t = 150 s, (d) t = 200 s, (e) t = 300 s, and (f) t = 400 s]. During each period, at constant blood flow (i.e., 0

    Techniques Used: In Vitro, Concentration Assay, Flow Cytometry

    Related Articles

    Flow Cytometry:

    Article Title: Condensin II protein dysfunction impacts mitochondrial respiration and mitochondrial oxidative stress responses
    Article Snippet: .. MitoTracker Cells were washed once with PBS and then incubated with 200 nM MitoTracker Green FM (Thermo Fisher Scientific, M7514) in RPMI medium without FBS for 30 min and flow cytometry analysis performed. .. After incubation, cells were washed with PBS and detached from the plate with cell dissociation buffer (Thermo Fisher Scientific, 131510140).

    Magnetic Beads:

    Article Title: Specificity tuning of antibody fragments to neutralize two human chemokines with a single agent
    Article Snippet: .. scFv phage libraries (1012 Pfu) were blocked with phosphate buffered saline (PBS) containing 3% (w/v) skimmed milk and then deselected on streptavidin magnetic beads (Dynal M-280). .. Deselected phage was incubated with NusA-hCXCL10 biotinylated fusion protein (10 nM for the first round of selection and 1 nM for the followings).

    Cytometry:

    Article Title: Condensin II protein dysfunction impacts mitochondrial respiration and mitochondrial oxidative stress responses
    Article Snippet: .. MitoTracker Cells were washed once with PBS and then incubated with 200 nM MitoTracker Green FM (Thermo Fisher Scientific, M7514) in RPMI medium without FBS for 30 min and flow cytometry analysis performed. .. After incubation, cells were washed with PBS and detached from the plate with cell dissociation buffer (Thermo Fisher Scientific, 131510140).

    Incubation:

    Article Title: The site of water stress governs the pattern of ABA synthesis and transport in peanut
    Article Snippet: .. After three 5 min washes with distilled water, the sections were blocked with 10% BSA in PBS, then incubated with a dilution (10 mM PBS, 1% BSA, pH 7.2) of anti-ABA monoclonal antibodies (1:2000 in 5% BSA/PBS; Invitrogen, CA, USA) and anti-AhNCED1 antibodies (our laboratory storage, Hu et al. ) overnight at 4 °C. .. Slides were rinsed three times with PBS and incubated with a biotin-labeled goat anti-rat IgG antibody for 20 min at 37 °C.

    Article Title: Condensin II protein dysfunction impacts mitochondrial respiration and mitochondrial oxidative stress responses
    Article Snippet: .. MitoTracker Cells were washed once with PBS and then incubated with 200 nM MitoTracker Green FM (Thermo Fisher Scientific, M7514) in RPMI medium without FBS for 30 min and flow cytometry analysis performed. .. After incubation, cells were washed with PBS and detached from the plate with cell dissociation buffer (Thermo Fisher Scientific, 131510140).

    Western Blot:

    Article Title: Tumor-Specific Delivery of Immune Checkpoint Inhibitors by Engineered AAV Vectors
    Article Snippet: .. Medium was exchanged after 24 h. For the detection and quantification of released antibodies by Western blot and nivolumab-ELISA, cells were washed in PBS before serum-free medium (virus production serum free medium, VPSFM, Thermo Fisher Scientific) was added. .. Four days after transduction, conditioned media were harvested, centrifuged at 300x g for 5–7 min to remove cell debris and stored at 4°C (or −80°C for long-term storage) for analysis.

    Staining:

    Article Title: Intrabodies against the Polysialyltransferases ST8SiaII and ST8SiaIV inhibit Polysialylation of NCAM in rhabdomyosarcoma tumor cells
    Article Snippet: .. Staining with antibodies was performed for 30 min at 4 °C in a 96-well microtitre plate (Nunclon™ Surface plate, Nunc) in 100 μl PBS containing 2% FCS (Invitrogen). .. For detection of the primary antibodies an goat anti-mouse RPE-labeled antibody (Dianova) was used.

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    Thermo Fisher hoechst 33342
    Dynamics of Bacteriocytes and Associated Nuclei during Whitefly Embryogenesis (A) Localization of bacteriocytes and associated nuclei in eggs at 0, 4, 5–6, 7, and 8 days post-oviposition, revealed by <t>Hoechst</t> 33342 staining of DNA. The bacterial symbionts that pack the cytoplasm of bacteriocytes are evident in the bacteriocyte periphery. bc, bacteriocyte; red arrow, bacteriocyte nucleus; green arrow, egg nucleus. (B) Bacteriocyte volume in eggs at 0, 4, 5–6, 7, and 8 days post-oviposition. Bacteriocyte volume was calculated from the diameter, assuming that the cell is a sphere, and it varied significantly with time (ANOVA: F 4,24 = 25.06, p
    Hoechst 33342, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hoechst 33342/product/Thermo Fisher
    Average 99 stars, based on 6136 article reviews
    Price from $9.99 to $1999.99
    hoechst 33342 - by Bioz Stars, 2020-08
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    93
    Thermo Fisher pbs casein solution
    Humoral and cellular immune responses of the mice from the prophylactic bioassay utilizing the oral cancer orthotopic model consisting of C3H/He mice challenged with the AT-84 E7 Luc cell line. Groups of five female C3H/He mice were immunized with one of the following treatments: 100 μ g of KLH or FLH, 50 μ g of KLH or FLH plus 10 μ g of QS-21, and 10 μ g of QS-21 or 100 μ l of <t>PBS.</t> At 10 and 25 days, serum samples were taken to determine the titers of <t>IgG</t> subclasses by indirect ELISA. DTH determination was performed on day 15 of the bioassay. (a) Total anti-hemocyanin IgG titers in mouse sera. Data are shown as means ± SEM. Anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with QS-21 at day 10 and at day 21. ∗∗∗∗ P
    Pbs Casein Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    94
    Thermo Fisher pbs solution
    Confocal micrographs showing the uptake by MCs of C6-LP ( A ), 6%-TRX-C6-LP ( B ), 11%-TRX-C6-LP ( C ), and 14%-TRX-C6-LP ( D ) after 24 h incubation (C6: green). Notes: MCs were incubated with C6-LP (control) or TRX-C6-LPs at a lipid concentration of 0.8 mg/mL for 2 h in serum-free RPMI 1640 at <t>37°C</t> and then washed with <t>PBS</t> to terminate the uptake process. After fixation in 10% neutral buffer formalin, the cells were counterstained with DAPI (blue) for observation under a laser confocal microscope. Magnification ×400. Abbreviations: C6, Coumarin-6; C6-LP, Coumarin-6-loaded liposomes; DAPI, dihydrochloride; MCs, mesangial cells; PBS, phosphate-buffered saline; TRX-C6-LP, TRX-20-modified Coumarin-6-loaded liposomes.
    Pbs Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 369 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs solution/product/Thermo Fisher
    Average 94 stars, based on 369 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Dynamics of Bacteriocytes and Associated Nuclei during Whitefly Embryogenesis (A) Localization of bacteriocytes and associated nuclei in eggs at 0, 4, 5–6, 7, and 8 days post-oviposition, revealed by Hoechst 33342 staining of DNA. The bacterial symbionts that pack the cytoplasm of bacteriocytes are evident in the bacteriocyte periphery. bc, bacteriocyte; red arrow, bacteriocyte nucleus; green arrow, egg nucleus. (B) Bacteriocyte volume in eggs at 0, 4, 5–6, 7, and 8 days post-oviposition. Bacteriocyte volume was calculated from the diameter, assuming that the cell is a sphere, and it varied significantly with time (ANOVA: F 4,24 = 25.06, p

    Journal: Current Biology

    Article Title: Maternal Inheritance of a Single Somatic Animal Cell Displayed by the Bacteriocyte in the Whitefly Bemisia tabaci

    doi: 10.1016/j.cub.2017.12.041

    Figure Lengend Snippet: Dynamics of Bacteriocytes and Associated Nuclei during Whitefly Embryogenesis (A) Localization of bacteriocytes and associated nuclei in eggs at 0, 4, 5–6, 7, and 8 days post-oviposition, revealed by Hoechst 33342 staining of DNA. The bacterial symbionts that pack the cytoplasm of bacteriocytes are evident in the bacteriocyte periphery. bc, bacteriocyte; red arrow, bacteriocyte nucleus; green arrow, egg nucleus. (B) Bacteriocyte volume in eggs at 0, 4, 5–6, 7, and 8 days post-oviposition. Bacteriocyte volume was calculated from the diameter, assuming that the cell is a sphere, and it varied significantly with time (ANOVA: F 4,24 = 25.06, p

    Article Snippet: Eggs deposited at day 0 were dechorionated by 60% Clorox bleach (3.6% hypochlorite) in PBS for 5 min and then wash with PBS twice, fixed by 4% paraformaldehyde (PFA) at room temperature for 1 h, and then permeabilized with 0.1% Triton X-100 in PBS for 1.5 h. The samples were incubated with Hoechst 33342 (10 μg ml-1 in PBS, Thermo Scientific) at room temperature for 20 min. After the dechorionation treatment, the nuclei in the embryos at late embryogenesis did not stain well.

    Techniques: Staining

    Humoral and cellular immune responses of the mice from the prophylactic bioassay utilizing the oral cancer orthotopic model consisting of C3H/He mice challenged with the AT-84 E7 Luc cell line. Groups of five female C3H/He mice were immunized with one of the following treatments: 100 μ g of KLH or FLH, 50 μ g of KLH or FLH plus 10 μ g of QS-21, and 10 μ g of QS-21 or 100 μ l of PBS. At 10 and 25 days, serum samples were taken to determine the titers of IgG subclasses by indirect ELISA. DTH determination was performed on day 15 of the bioassay. (a) Total anti-hemocyanin IgG titers in mouse sera. Data are shown as means ± SEM. Anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with QS-21 at day 10 and at day 21. ∗∗∗∗ P

    Journal: Journal of Immunology Research

    Article Title: Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer

    doi: 10.1155/2019/7076942

    Figure Lengend Snippet: Humoral and cellular immune responses of the mice from the prophylactic bioassay utilizing the oral cancer orthotopic model consisting of C3H/He mice challenged with the AT-84 E7 Luc cell line. Groups of five female C3H/He mice were immunized with one of the following treatments: 100 μ g of KLH or FLH, 50 μ g of KLH or FLH plus 10 μ g of QS-21, and 10 μ g of QS-21 or 100 μ l of PBS. At 10 and 25 days, serum samples were taken to determine the titers of IgG subclasses by indirect ELISA. DTH determination was performed on day 15 of the bioassay. (a) Total anti-hemocyanin IgG titers in mouse sera. Data are shown as means ± SEM. Anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with QS-21 at day 10 and at day 21. ∗∗∗∗ P

    Article Snippet: Subsequently, 100 μ l of a 1% PBS-casein solution containing goat anti-mouse IgG (H + L) serum labeled with alkaline phosphatase (ALP; Thermo Scientific) was added at a 1 : 2500 dilution and incubated for 30 minutes at 37°C.

    Techniques: Mouse Assay, Indirect ELISA

    Specific humoral and cellular immune responses in C57BL/6 mice immunized with hemocyanins in combination with adjuvants. Groups of three female C57BL/6 mice were immunized subcutaneously on days 1 and 16 with one of the following treatments: 50 μ g of KLH, CCH, or FLH alone or in combination with 100 μ g of alum or 10 μ g of QS-21 in a 1 : 1 (vol/vol) ratio with AddaVax, all in a total volume of 100 μ l of PBS (vehicle). A serum sample was taken from each animal on day 37 after the second immunization for analysis by indirect ELISA. (a) Total anti-hemocyanin IgG titers in mouse sera. Values are presented as means ± SEM. The anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with an adjuvant. ∗∗∗∗ P

    Journal: Journal of Immunology Research

    Article Title: Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer

    doi: 10.1155/2019/7076942

    Figure Lengend Snippet: Specific humoral and cellular immune responses in C57BL/6 mice immunized with hemocyanins in combination with adjuvants. Groups of three female C57BL/6 mice were immunized subcutaneously on days 1 and 16 with one of the following treatments: 50 μ g of KLH, CCH, or FLH alone or in combination with 100 μ g of alum or 10 μ g of QS-21 in a 1 : 1 (vol/vol) ratio with AddaVax, all in a total volume of 100 μ l of PBS (vehicle). A serum sample was taken from each animal on day 37 after the second immunization for analysis by indirect ELISA. (a) Total anti-hemocyanin IgG titers in mouse sera. Values are presented as means ± SEM. The anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with an adjuvant. ∗∗∗∗ P

    Article Snippet: Subsequently, 100 μ l of a 1% PBS-casein solution containing goat anti-mouse IgG (H + L) serum labeled with alkaline phosphatase (ALP; Thermo Scientific) was added at a 1 : 2500 dilution and incubated for 30 minutes at 37°C.

    Techniques: Mouse Assay, Indirect ELISA

    Confocal micrographs showing the uptake by MCs of C6-LP ( A ), 6%-TRX-C6-LP ( B ), 11%-TRX-C6-LP ( C ), and 14%-TRX-C6-LP ( D ) after 24 h incubation (C6: green). Notes: MCs were incubated with C6-LP (control) or TRX-C6-LPs at a lipid concentration of 0.8 mg/mL for 2 h in serum-free RPMI 1640 at 37°C and then washed with PBS to terminate the uptake process. After fixation in 10% neutral buffer formalin, the cells were counterstained with DAPI (blue) for observation under a laser confocal microscope. Magnification ×400. Abbreviations: C6, Coumarin-6; C6-LP, Coumarin-6-loaded liposomes; DAPI, dihydrochloride; MCs, mesangial cells; PBS, phosphate-buffered saline; TRX-C6-LP, TRX-20-modified Coumarin-6-loaded liposomes.

    Journal: International Journal of Nanomedicine

    Article Title: Renal-targeted delivery of triptolide by entrapment in pegylated TRX-20-modified liposomes

    doi: 10.2147/IJN.S141095

    Figure Lengend Snippet: Confocal micrographs showing the uptake by MCs of C6-LP ( A ), 6%-TRX-C6-LP ( B ), 11%-TRX-C6-LP ( C ), and 14%-TRX-C6-LP ( D ) after 24 h incubation (C6: green). Notes: MCs were incubated with C6-LP (control) or TRX-C6-LPs at a lipid concentration of 0.8 mg/mL for 2 h in serum-free RPMI 1640 at 37°C and then washed with PBS to terminate the uptake process. After fixation in 10% neutral buffer formalin, the cells were counterstained with DAPI (blue) for observation under a laser confocal microscope. Magnification ×400. Abbreviations: C6, Coumarin-6; C6-LP, Coumarin-6-loaded liposomes; DAPI, dihydrochloride; MCs, mesangial cells; PBS, phosphate-buffered saline; TRX-C6-LP, TRX-20-modified Coumarin-6-loaded liposomes.

    Article Snippet: After enzymatic digestions with collagenase IV (0.1% w/v) at 37°C in PBS solution for 20–45 min, the MC suspensions were obtained and cultured in RPMI 1640 medium containing 20% heat-inactivated fetal bovine serum, 2 μg/mL insulin, 300 μg/L transferrin, 100 U/mL penicillin, and 100 U/mL streptomycin at 37°C in a humidified 5% (v/v) CO2 incubator (Thermo Scientific, Marietta, OH, USA).

    Techniques: Incubation, Concentration Assay, Microscopy, Modification