phosphate buffered saline pbs solution  (Thermo Fisher)


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  • 99
    Name:
    Phosphate Buffered Saline
    Description:
    Recover microorganisms from multiple sample types including rinse samples from clinical instruments and environmental monitoring samples by creating a pH balanced environment with Thermo Scientific Phosphate Buffered Saline with Poly 80 PBST a buffer solution commonly used in biological research
    Catalog Number:
    r112502
    Price:
    None
    Applications:
    Blood Culture|Clinical|Clinical Microbiology
    Category:
    Cell Culture Transfection Reagents
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    Structured Review

    Thermo Fisher phosphate buffered saline pbs solution
    Inertio-elastic focusing of bioparticles based on deformability and shape ( a ) Deformation statistics of WBCs in <t>PBS,</t> a low-molecular weight (357 <t>kDa)</t> HA solution and a high-molecular weight (1,650 kDa) HA solution. The magnitude of WBC stretching is expressed in terms of aspect ratio AR = a x /a z . Scale bar, 10 µm. The error bars indicate the s.d. in the WBC aspect ratio at each flow rate. ( b ) LEF and particle trajectory analysis images of WBCs in PBS, 357 kDa HA solution and 1,650 kDa HA solution at Q = 13 ml min −1 . ( c ) Particle trajectory analysis (PTA) images of anisotropic PEG particles in 1,650 kDa HA solution at Q = 20 ml min −1 . Dashed red lines indicate channel centerline. Scale bar, 30 µm. Measurements of lateral position z and instantaneous orientation angle θ are plotted for each PEG particle in water (blue) and in the HA solution (green).
    Recover microorganisms from multiple sample types including rinse samples from clinical instruments and environmental monitoring samples by creating a pH balanced environment with Thermo Scientific Phosphate Buffered Saline with Poly 80 PBST a buffer solution commonly used in biological research
    https://www.bioz.com/result/phosphate buffered saline pbs solution/product/Thermo Fisher
    Average 99 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    phosphate buffered saline pbs solution - by Bioz Stars, 2021-01
    99/100 stars

    Images

    1) Product Images from "Inertio-elastic focusing of bioparticles in microchannels at high throughput"

    Article Title: Inertio-elastic focusing of bioparticles in microchannels at high throughput

    Journal: Nature communications

    doi: 10.1038/ncomms5120

    Inertio-elastic focusing of bioparticles based on deformability and shape ( a ) Deformation statistics of WBCs in PBS, a low-molecular weight (357 kDa) HA solution and a high-molecular weight (1,650 kDa) HA solution. The magnitude of WBC stretching is expressed in terms of aspect ratio AR = a x /a z . Scale bar, 10 µm. The error bars indicate the s.d. in the WBC aspect ratio at each flow rate. ( b ) LEF and particle trajectory analysis images of WBCs in PBS, 357 kDa HA solution and 1,650 kDa HA solution at Q = 13 ml min −1 . ( c ) Particle trajectory analysis (PTA) images of anisotropic PEG particles in 1,650 kDa HA solution at Q = 20 ml min −1 . Dashed red lines indicate channel centerline. Scale bar, 30 µm. Measurements of lateral position z and instantaneous orientation angle θ are plotted for each PEG particle in water (blue) and in the HA solution (green).
    Figure Legend Snippet: Inertio-elastic focusing of bioparticles based on deformability and shape ( a ) Deformation statistics of WBCs in PBS, a low-molecular weight (357 kDa) HA solution and a high-molecular weight (1,650 kDa) HA solution. The magnitude of WBC stretching is expressed in terms of aspect ratio AR = a x /a z . Scale bar, 10 µm. The error bars indicate the s.d. in the WBC aspect ratio at each flow rate. ( b ) LEF and particle trajectory analysis images of WBCs in PBS, 357 kDa HA solution and 1,650 kDa HA solution at Q = 13 ml min −1 . ( c ) Particle trajectory analysis (PTA) images of anisotropic PEG particles in 1,650 kDa HA solution at Q = 20 ml min −1 . Dashed red lines indicate channel centerline. Scale bar, 30 µm. Measurements of lateral position z and instantaneous orientation angle θ are plotted for each PEG particle in water (blue) and in the HA solution (green).

    Techniques Used: Molecular Weight, Flow Cytometry

    2) Product Images from "Periodic and simultaneous quantification of blood viscosity and red blood cell aggregation using a microfluidic platform under in-vitro closed-loop circulation"

    Article Title: Periodic and simultaneous quantification of blood viscosity and red blood cell aggregation using a microfluidic platform under in-vitro closed-loop circulation

    Journal: Biomicrofluidics

    doi: 10.1063/1.5017052

    Schematics for the periodic and simultaneous measurement of blood viscosity RBC aggregation using a microfluidic platform under in-vitro closed-loop circulation. (A) Schematics of the experimental setup including an in-vitro closed-loop circulation and a microfluidic platform. (a) In-vitro closed-loop circulation established by connecting several components (peristaltic pump, ACU, FD, and reservoir) with polyethylene tubes (L 5 , L 6 , L 7 , L 8 , and L 9 ) in series. After the reservoir was filled with blood (10 ml), a peristaltic pump was set to ω = 15 rpm. The air cavity in the ACU was adjusted to 2.5 ml. A specific concentration of dextran solution (i.e., C dextran = 100 mg/ml) was delivered into the reservoir by operating the syringe pump. (b) A microfluidic platform including a microfluidic device (VA), PV, and syringe pump. The outlet of the FD was connected to the inlet (B) of the VA with a polyethylene tube (L 2 ). To control the blood flow from the FD to the VA, a PV was installed in front of the inlet (B). 1× PBS solution was supplied into inlet (A) with a syringe pump. (B) Flow-rate profile of two fluids with a syringe pump (i.e., Q PBS and Q Dextran ) and PV operation (i.e., open and close) for each period. (C) Quantification of image intensity (⟨I⟩) and blood flow-rate (Q μ PIV ). (a) Quantification of the image intensity (⟨I⟩) within the ROI (484 × 150 pixels) in the upper channel. (b) Quantification of blood velocity fields with a time-resolved micro-PIV technique. The blood flow-rate (Q μ PIV ) was quantified by multiplying averaged blood velocity (⟨U⟩) by the cross-sectional area (A c ) (i.e., Q μ PIV = ⟨U⟩·A c ). (c) A simple fluidic circuit with discrete elements including fluidic resistances (R P , R B ), and flow rates (Q PBS , Q Blood ). The interface between two fluids was evaluated by converting the gray image into a binary image. (D) For a preliminary demonstration, blood (Hct = 50%) was prepared by adding normal RBCs to autologous plasma. Here, the period was set to T = 400 s. The inset shows microscopic images at a specific time (t) [(a) t = 10 s, (b) t = 100 s, (c) t = 150 s, (d) t = 200 s, (e) t = 300 s, and (f) t = 400 s]. During each period, at constant blood flow (i.e., 0
    Figure Legend Snippet: Schematics for the periodic and simultaneous measurement of blood viscosity RBC aggregation using a microfluidic platform under in-vitro closed-loop circulation. (A) Schematics of the experimental setup including an in-vitro closed-loop circulation and a microfluidic platform. (a) In-vitro closed-loop circulation established by connecting several components (peristaltic pump, ACU, FD, and reservoir) with polyethylene tubes (L 5 , L 6 , L 7 , L 8 , and L 9 ) in series. After the reservoir was filled with blood (10 ml), a peristaltic pump was set to ω = 15 rpm. The air cavity in the ACU was adjusted to 2.5 ml. A specific concentration of dextran solution (i.e., C dextran = 100 mg/ml) was delivered into the reservoir by operating the syringe pump. (b) A microfluidic platform including a microfluidic device (VA), PV, and syringe pump. The outlet of the FD was connected to the inlet (B) of the VA with a polyethylene tube (L 2 ). To control the blood flow from the FD to the VA, a PV was installed in front of the inlet (B). 1× PBS solution was supplied into inlet (A) with a syringe pump. (B) Flow-rate profile of two fluids with a syringe pump (i.e., Q PBS and Q Dextran ) and PV operation (i.e., open and close) for each period. (C) Quantification of image intensity (⟨I⟩) and blood flow-rate (Q μ PIV ). (a) Quantification of the image intensity (⟨I⟩) within the ROI (484 × 150 pixels) in the upper channel. (b) Quantification of blood velocity fields with a time-resolved micro-PIV technique. The blood flow-rate (Q μ PIV ) was quantified by multiplying averaged blood velocity (⟨U⟩) by the cross-sectional area (A c ) (i.e., Q μ PIV = ⟨U⟩·A c ). (c) A simple fluidic circuit with discrete elements including fluidic resistances (R P , R B ), and flow rates (Q PBS , Q Blood ). The interface between two fluids was evaluated by converting the gray image into a binary image. (D) For a preliminary demonstration, blood (Hct = 50%) was prepared by adding normal RBCs to autologous plasma. Here, the period was set to T = 400 s. The inset shows microscopic images at a specific time (t) [(a) t = 10 s, (b) t = 100 s, (c) t = 150 s, (d) t = 200 s, (e) t = 300 s, and (f) t = 400 s]. During each period, at constant blood flow (i.e., 0

    Techniques Used: In Vitro, Concentration Assay, Flow Cytometry

    Related Articles

    Fluorescence:

    Article Title: Identification of Major Capsid Protein as a Potential Biomarker of Grouper Iridovirus-Infected Cells Using Aptamers Selected by SELEX
    Article Snippet: .. Co-localization of Target and Aptamer by Confocal Fluorescence Imaging TAMRA-labeled aptamer Q5c was incubating with SGIV-infected with GS cells in 35-mm glass-bottom culture dish (Cellvis) at 4°C for 40 min. After being washed three times with PBS, cells were incubated with anti-MCP serum and FITC-labeled goat anti-mouse antibodies (FITC-labeled anti-MCP, Thermo Fisher Scientific) for immunofluorescence staining, as described previously ( ). .. TAMRA-labeled random ssDNA library (Lib) incubated with SGIV-infected GS cells served as the control group.

    Quantitative RT-PCR:

    Article Title: Infection and Rapid Transmission of SARS-CoV-2 in Ferrets
    Article Snippet: .. Quantitative Real-Time RT-PCR (qRT-PCR) to Detect SARS-CoV-2 RNA Collected ferret secretions were resuspended with cold phosphate-buffered saline (PBS) containing antibiotics (5% penicillin/streptomycin; GIBCO). .. For virus titration, total RNA was extracted from the collected samples using the RNeasy Mini® kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions.

    Mouse Assay:

    Article Title: CD4+ Virtual Memory: Antigen-inexperienced T cells reside in the naïve, regulatory, and memory T cell compartments at similar frequencies, implications for autoimmunity
    Article Snippet: .. Female BALB/c mice were immunized subcutaneously with 10μg of HEL (Sigma) or PBS (Gibco) emulsified in CFA (DIFCO) or IFA. .. Draining lymph nodes were harvested and single-cell suspensions were prepared in petri dishes containing DME (GIBCO).

    Incubation:

    Article Title: Identification of Major Capsid Protein as a Potential Biomarker of Grouper Iridovirus-Infected Cells Using Aptamers Selected by SELEX
    Article Snippet: .. Co-localization of Target and Aptamer by Confocal Fluorescence Imaging TAMRA-labeled aptamer Q5c was incubating with SGIV-infected with GS cells in 35-mm glass-bottom culture dish (Cellvis) at 4°C for 40 min. After being washed three times with PBS, cells were incubated with anti-MCP serum and FITC-labeled goat anti-mouse antibodies (FITC-labeled anti-MCP, Thermo Fisher Scientific) for immunofluorescence staining, as described previously ( ). .. TAMRA-labeled random ssDNA library (Lib) incubated with SGIV-infected GS cells served as the control group.

    Article Title: Novel ?-Secretase Cleavage of ?-Amyloid Precursor Protein in the Endoplasmic Reticulum/Intermediate Compartment of NT2N Cells
    Article Snippet: .. After rinsing the cultures twice with PBS, the NT2N neurons were incubated on ice for 20 min with PBS, with 10 μg/ml of trypsin in PBS alone (Life Technologies, Inc.), or with 10 μg/ml trypsin and 0.1% Triton X-100 in PBS. ..

    Article Title: High Fat Diet Causes Depletion of Intestinal Eosinophils Associated with Intestinal Permeability
    Article Snippet: .. 2 x 106 cells were then incubated with 1:1000 dilution of Live dead aqua (Invitrogen, NY) and 1:100 dilution of anti CD16/32 antibody (eBioscience, San Diego, CA, 93) for 30 minutes on ice in PBS, washed twice and incubated with the following antibody panel in PBS containing 2%FCS, 2mM EDTA: CD45 NC605 (30-F11), F4/80PECy7 (BM8) (from eBioscience, San Diego, CA), CD11b-FITC (M170), Siglec F-PE (E50-2440), CD11c-APC or CD11c-v450 (HL3) (from BD Biosciences, San Jose, CA) and MHCII APCCy7 (Biolegend, San Diego, CA, M5/114.15.2). .. Cells were fixed overnight in BD stabilizing fixative (BD Biosciences, San Jose, CA), washed twice and acquired on a BD Canto RUO flow cytometer.

    Imaging:

    Article Title: Identification of Major Capsid Protein as a Potential Biomarker of Grouper Iridovirus-Infected Cells Using Aptamers Selected by SELEX
    Article Snippet: .. Co-localization of Target and Aptamer by Confocal Fluorescence Imaging TAMRA-labeled aptamer Q5c was incubating with SGIV-infected with GS cells in 35-mm glass-bottom culture dish (Cellvis) at 4°C for 40 min. After being washed three times with PBS, cells were incubated with anti-MCP serum and FITC-labeled goat anti-mouse antibodies (FITC-labeled anti-MCP, Thermo Fisher Scientific) for immunofluorescence staining, as described previously ( ). .. TAMRA-labeled random ssDNA library (Lib) incubated with SGIV-infected GS cells served as the control group.

    Staining:

    Article Title: Identification of Major Capsid Protein as a Potential Biomarker of Grouper Iridovirus-Infected Cells Using Aptamers Selected by SELEX
    Article Snippet: .. Co-localization of Target and Aptamer by Confocal Fluorescence Imaging TAMRA-labeled aptamer Q5c was incubating with SGIV-infected with GS cells in 35-mm glass-bottom culture dish (Cellvis) at 4°C for 40 min. After being washed three times with PBS, cells were incubated with anti-MCP serum and FITC-labeled goat anti-mouse antibodies (FITC-labeled anti-MCP, Thermo Fisher Scientific) for immunofluorescence staining, as described previously ( ). .. TAMRA-labeled random ssDNA library (Lib) incubated with SGIV-infected GS cells served as the control group.

    Immunofluorescence:

    Article Title: Identification of Major Capsid Protein as a Potential Biomarker of Grouper Iridovirus-Infected Cells Using Aptamers Selected by SELEX
    Article Snippet: .. Co-localization of Target and Aptamer by Confocal Fluorescence Imaging TAMRA-labeled aptamer Q5c was incubating with SGIV-infected with GS cells in 35-mm glass-bottom culture dish (Cellvis) at 4°C for 40 min. After being washed three times with PBS, cells were incubated with anti-MCP serum and FITC-labeled goat anti-mouse antibodies (FITC-labeled anti-MCP, Thermo Fisher Scientific) for immunofluorescence staining, as described previously ( ). .. TAMRA-labeled random ssDNA library (Lib) incubated with SGIV-infected GS cells served as the control group.

    Article Title: CD4+ Virtual Memory: Antigen-inexperienced T cells reside in the naïve, regulatory, and memory T cell compartments at similar frequencies, implications for autoimmunity
    Article Snippet: .. Female BALB/c mice were immunized subcutaneously with 10μg of HEL (Sigma) or PBS (Gibco) emulsified in CFA (DIFCO) or IFA. .. Draining lymph nodes were harvested and single-cell suspensions were prepared in petri dishes containing DME (GIBCO).

    SPR Assay:

    Article Title: Promiscuous Binding of Karyopherinβ1 Modulates FG Nucleoporin Barrier Function and Expedites NTF2 Transport Kinetics
    Article Snippet: .. A four-flow cell Biacore instrument (T100; GE Healthcare) was used to measure SPR at 25°C in PBS, pH 7.2 (GIBCO by Life Technologies), as previously detailed ( ). ..

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  • 99
    Thermo Fisher to pro 3 iodide
    Endogenous PRA1 does not strictly localize to the Golgi in mature C57Bl/6J photoreceptor cells. (A) Schematic showing PRA1 topology. (B) PRA1 partially co-localizes with both the Golgi marker GM130, and the ribbon synapse marker Ribeye (C) as indicated by solid arrowheads. Images are representative of three non-adjacent sections from four retinas and are compiled from a single 1 μm z-section. Insets are nine-fold magnifications of an area within the displayed images, highlighted with a dashed-line. Scale bar: 10 μm. Green: PRA1, Magenta: Organelle markers, Blue: Nuclear labeling via <t>TO-PRO-3,</t> White: Co-localization, OS: Outer segment, IS: Inner segment, ONL: Outer nuclear layer, OPL: Outer plexiform layer.
    To Pro 3 Iodide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/to pro 3 iodide/product/Thermo Fisher
    Average 99 stars, based on 142 article reviews
    Price from $9.99 to $1999.99
    to pro 3 iodide - by Bioz Stars, 2021-01
    99/100 stars
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    92
    Thermo Fisher pbs casein solution
    Humoral and cellular immune responses of the mice from the prophylactic bioassay utilizing the oral cancer orthotopic model consisting of C3H/He mice challenged with the AT-84 E7 Luc cell line. Groups of five female C3H/He mice were immunized with one of the following treatments: 100 μ g of KLH or FLH, 50 μ g of KLH or FLH plus 10 μ g of QS-21, and 10 μ g of QS-21 or 100 μ l of <t>PBS.</t> At 10 and 25 days, serum samples were taken to determine the titers of <t>IgG</t> subclasses by indirect ELISA. DTH determination was performed on day 15 of the bioassay. (a) Total anti-hemocyanin IgG titers in mouse sera. Data are shown as means ± SEM. Anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with QS-21 at day 10 and at day 21. ∗∗∗∗ P
    Pbs Casein Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs casein solution/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbs casein solution - by Bioz Stars, 2021-01
    92/100 stars
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    92
    Thermo Fisher pbs solution
    Confocal micrographs showing the uptake by MCs of C6-LP ( A ), 6%-TRX-C6-LP ( B ), 11%-TRX-C6-LP ( C ), and 14%-TRX-C6-LP ( D ) after 24 h incubation (C6: green). Notes: MCs were incubated with C6-LP (control) or TRX-C6-LPs at a lipid concentration of 0.8 mg/mL for 2 h in serum-free RPMI 1640 at <t>37°C</t> and then washed with <t>PBS</t> to terminate the uptake process. After fixation in 10% neutral buffer formalin, the cells were counterstained with DAPI (blue) for observation under a laser confocal microscope. Magnification ×400. Abbreviations: C6, Coumarin-6; C6-LP, Coumarin-6-loaded liposomes; DAPI, dihydrochloride; MCs, mesangial cells; PBS, phosphate-buffered saline; TRX-C6-LP, TRX-20-modified Coumarin-6-loaded liposomes.
    Pbs Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 369 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs solution/product/Thermo Fisher
    Average 92 stars, based on 369 article reviews
    Price from $9.99 to $1999.99
    pbs solution - by Bioz Stars, 2021-01
    92/100 stars
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    Image Search Results


    Endogenous PRA1 does not strictly localize to the Golgi in mature C57Bl/6J photoreceptor cells. (A) Schematic showing PRA1 topology. (B) PRA1 partially co-localizes with both the Golgi marker GM130, and the ribbon synapse marker Ribeye (C) as indicated by solid arrowheads. Images are representative of three non-adjacent sections from four retinas and are compiled from a single 1 μm z-section. Insets are nine-fold magnifications of an area within the displayed images, highlighted with a dashed-line. Scale bar: 10 μm. Green: PRA1, Magenta: Organelle markers, Blue: Nuclear labeling via TO-PRO-3, White: Co-localization, OS: Outer segment, IS: Inner segment, ONL: Outer nuclear layer, OPL: Outer plexiform layer.

    Journal: PLoS ONE

    Article Title: Di-arginine and FFAT-like motifs retain a subpopulation of PRA1 at ER-mitochondria membrane contact sites

    doi: 10.1371/journal.pone.0243075

    Figure Lengend Snippet: Endogenous PRA1 does not strictly localize to the Golgi in mature C57Bl/6J photoreceptor cells. (A) Schematic showing PRA1 topology. (B) PRA1 partially co-localizes with both the Golgi marker GM130, and the ribbon synapse marker Ribeye (C) as indicated by solid arrowheads. Images are representative of three non-adjacent sections from four retinas and are compiled from a single 1 μm z-section. Insets are nine-fold magnifications of an area within the displayed images, highlighted with a dashed-line. Scale bar: 10 μm. Green: PRA1, Magenta: Organelle markers, Blue: Nuclear labeling via TO-PRO-3, White: Co-localization, OS: Outer segment, IS: Inner segment, ONL: Outer nuclear layer, OPL: Outer plexiform layer.

    Article Snippet: For retinal sections, after the application of secondary antibodies, the nucleus was stained with TO-PRO-3 iodide (1:500 in phosphate buffered saline (PBS), ThermoFisher Scientific Cat #T3605) for 15 minutes.

    Techniques: Marker, Labeling

    Humoral and cellular immune responses of the mice from the prophylactic bioassay utilizing the oral cancer orthotopic model consisting of C3H/He mice challenged with the AT-84 E7 Luc cell line. Groups of five female C3H/He mice were immunized with one of the following treatments: 100 μ g of KLH or FLH, 50 μ g of KLH or FLH plus 10 μ g of QS-21, and 10 μ g of QS-21 or 100 μ l of PBS. At 10 and 25 days, serum samples were taken to determine the titers of IgG subclasses by indirect ELISA. DTH determination was performed on day 15 of the bioassay. (a) Total anti-hemocyanin IgG titers in mouse sera. Data are shown as means ± SEM. Anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with QS-21 at day 10 and at day 21. ∗∗∗∗ P

    Journal: Journal of Immunology Research

    Article Title: Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer

    doi: 10.1155/2019/7076942

    Figure Lengend Snippet: Humoral and cellular immune responses of the mice from the prophylactic bioassay utilizing the oral cancer orthotopic model consisting of C3H/He mice challenged with the AT-84 E7 Luc cell line. Groups of five female C3H/He mice were immunized with one of the following treatments: 100 μ g of KLH or FLH, 50 μ g of KLH or FLH plus 10 μ g of QS-21, and 10 μ g of QS-21 or 100 μ l of PBS. At 10 and 25 days, serum samples were taken to determine the titers of IgG subclasses by indirect ELISA. DTH determination was performed on day 15 of the bioassay. (a) Total anti-hemocyanin IgG titers in mouse sera. Data are shown as means ± SEM. Anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with QS-21 at day 10 and at day 21. ∗∗∗∗ P

    Article Snippet: Subsequently, 100 μ l of a 1% PBS-casein solution containing goat anti-mouse IgG (H + L) serum labeled with alkaline phosphatase (ALP; Thermo Scientific) was added at a 1 : 2500 dilution and incubated for 30 minutes at 37°C.

    Techniques: Mouse Assay, Indirect ELISA

    Specific humoral and cellular immune responses in C57BL/6 mice immunized with hemocyanins in combination with adjuvants. Groups of three female C57BL/6 mice were immunized subcutaneously on days 1 and 16 with one of the following treatments: 50 μ g of KLH, CCH, or FLH alone or in combination with 100 μ g of alum or 10 μ g of QS-21 in a 1 : 1 (vol/vol) ratio with AddaVax, all in a total volume of 100 μ l of PBS (vehicle). A serum sample was taken from each animal on day 37 after the second immunization for analysis by indirect ELISA. (a) Total anti-hemocyanin IgG titers in mouse sera. Values are presented as means ± SEM. The anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with an adjuvant. ∗∗∗∗ P

    Journal: Journal of Immunology Research

    Article Title: Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer

    doi: 10.1155/2019/7076942

    Figure Lengend Snippet: Specific humoral and cellular immune responses in C57BL/6 mice immunized with hemocyanins in combination with adjuvants. Groups of three female C57BL/6 mice were immunized subcutaneously on days 1 and 16 with one of the following treatments: 50 μ g of KLH, CCH, or FLH alone or in combination with 100 μ g of alum or 10 μ g of QS-21 in a 1 : 1 (vol/vol) ratio with AddaVax, all in a total volume of 100 μ l of PBS (vehicle). A serum sample was taken from each animal on day 37 after the second immunization for analysis by indirect ELISA. (a) Total anti-hemocyanin IgG titers in mouse sera. Values are presented as means ± SEM. The anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with an adjuvant. ∗∗∗∗ P

    Article Snippet: Subsequently, 100 μ l of a 1% PBS-casein solution containing goat anti-mouse IgG (H + L) serum labeled with alkaline phosphatase (ALP; Thermo Scientific) was added at a 1 : 2500 dilution and incubated for 30 minutes at 37°C.

    Techniques: Mouse Assay, Indirect ELISA

    Confocal micrographs showing the uptake by MCs of C6-LP ( A ), 6%-TRX-C6-LP ( B ), 11%-TRX-C6-LP ( C ), and 14%-TRX-C6-LP ( D ) after 24 h incubation (C6: green). Notes: MCs were incubated with C6-LP (control) or TRX-C6-LPs at a lipid concentration of 0.8 mg/mL for 2 h in serum-free RPMI 1640 at 37°C and then washed with PBS to terminate the uptake process. After fixation in 10% neutral buffer formalin, the cells were counterstained with DAPI (blue) for observation under a laser confocal microscope. Magnification ×400. Abbreviations: C6, Coumarin-6; C6-LP, Coumarin-6-loaded liposomes; DAPI, dihydrochloride; MCs, mesangial cells; PBS, phosphate-buffered saline; TRX-C6-LP, TRX-20-modified Coumarin-6-loaded liposomes.

    Journal: International Journal of Nanomedicine

    Article Title: Renal-targeted delivery of triptolide by entrapment in pegylated TRX-20-modified liposomes

    doi: 10.2147/IJN.S141095

    Figure Lengend Snippet: Confocal micrographs showing the uptake by MCs of C6-LP ( A ), 6%-TRX-C6-LP ( B ), 11%-TRX-C6-LP ( C ), and 14%-TRX-C6-LP ( D ) after 24 h incubation (C6: green). Notes: MCs were incubated with C6-LP (control) or TRX-C6-LPs at a lipid concentration of 0.8 mg/mL for 2 h in serum-free RPMI 1640 at 37°C and then washed with PBS to terminate the uptake process. After fixation in 10% neutral buffer formalin, the cells were counterstained with DAPI (blue) for observation under a laser confocal microscope. Magnification ×400. Abbreviations: C6, Coumarin-6; C6-LP, Coumarin-6-loaded liposomes; DAPI, dihydrochloride; MCs, mesangial cells; PBS, phosphate-buffered saline; TRX-C6-LP, TRX-20-modified Coumarin-6-loaded liposomes.

    Article Snippet: After enzymatic digestions with collagenase IV (0.1% w/v) at 37°C in PBS solution for 20–45 min, the MC suspensions were obtained and cultured in RPMI 1640 medium containing 20% heat-inactivated fetal bovine serum, 2 μg/mL insulin, 300 μg/L transferrin, 100 U/mL penicillin, and 100 U/mL streptomycin at 37°C in a humidified 5% (v/v) CO2 incubator (Thermo Scientific, Marietta, OH, USA).

    Techniques: Incubation, Concentration Assay, Microscopy, Modification