phosphate buffered saline pbs solution  (GE Healthcare)

 
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    GE Healthcare phosphate buffered saline pbs solution
    Phosphate Buffered Saline Pbs Solution, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffered saline pbs solution/product/GE Healthcare
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    phosphate buffered saline pbs solution - by Bioz Stars, 2020-08
    92/100 stars

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    Related Articles

    Modification:

    Article Title: Reduced graphene oxide triggered epithelial-mesenchymal transition in A549 cells
    Article Snippet: .. Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DME/F-12) and phosphate-buffered saline (PBS) solution were obtained from HyClone (Logan, Utah, USA). .. Fetal bovine serum (FBS)was from Gibco Invitrogen (CA, USA).

    other:

    Article Title: Bioinspired neuron-like electronics
    Article Snippet: Released NeuE probes were rinsed with deionized (DI) water, soaked in an aqueous solution of poly-D-lysine (1.0 mg/ml, MW 70,000– 150,000, Sigma-Aldrich) for 24 h and then transferred to 1× phosphate buffered saline (PBS) solution (HyClone, GE Healthcare Life Sciences).

    Article Title: In Vivo Gene Activity of Human Mesenchymal Stem Cells After Scaffold-Mediated Local Transplantation
    Article Snippet: The marrow suspension was collected in a syringe containing 6000 U/mL heparin and was mixed with phosphate-buffered saline (PBS) solution in a 1:1 volume ratio and centrifuged at 2500 rpm for 10 min. After aspiration of the upper PBS layer, the marrow suspension was layered on Ficoll-paque (Amersham Biosciences, Uppsala, Sweden) in a 1:5 ratio and centrifuged at 1200 g for 30 min. Nucleated cells concentrated at the interface were collected and washed with PBS.

    Article Title: Differential Protein Expression between Chondrogenic Differentiated MSCs, Undifferentiated MSCs and Adult Chondroctyes Derived from Oryctolagus cuniculus in vitro
    Article Snippet: Briefly, 2 mL of bone marrow was diluted with Phosphate buffered saline (PBS) solution (1:2) before being loaded onto 3 mL Ficoll-paque (GE Healthcare-Amersham Biosciences, Piscataway, NJ).

    Article Title: Impact of collection, isolation and storage methodology of circulating microvesicles on flow cytometric analysis
    Article Snippet: Phosphate-buffered saline (PBS) solution (SH30256.01B) was purchased from HyClone Corporation (Logan, UT, USA), and filtered through a 0.22-µm filter (EMD Millipore, Billerica, MA, USA) to minimize interference from particles in PBS.

    Article Title: Combinatorial Co-Encapsulation of Hydrophobic Molecules in Poly(lactide-co-glycolide) Microparticles
    Article Snippet: Phosphate buffered saline (PBS) solution (Hyclone, UT, USA) was used as the aqueous phase to form the emulsions while propylene carbonate (PC) (Fisher Scientific, NJ, USA) was used as an organic solvent to dissolve PLGA polymer.

    Imaging:

    Article Title: Five colour variants of bright luminescent protein for real-time multicolour bioimaging
    Article Snippet: .. Initially, the E. coli colonies were poured with a phosphate-buffered saline (PBS) solution supplemented with 5 μM coelenterazine-h and examined with an LAS-1000 luminescence imaging system (GE Healthcare). ..

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    • strep tactin hrp detection solution  (GE Healthcare)
    90
    GE Healthcare strep tactin hrp detection solution
    Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a <t>Strep-Tactin®-HRP</t> conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).
    Strep Tactin Hrp Detection Solution, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/strep tactin hrp detection solution/product/GE Healthcare
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    strep tactin hrp detection solution - by Bioz Stars, 2020-08
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    bovine serum albumin solution  (GE Healthcare)
    91
    GE Healthcare bovine serum albumin solution
    Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a <t>Strep-Tactin®-HRP</t> conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).
    Bovine Serum Albumin Solution, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bovine serum albumin solution/product/GE Healthcare
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bovine serum albumin solution - by Bioz Stars, 2020-08
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    pbs solution  (GE Healthcare)
    92
    GE Healthcare pbs solution
    Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a <t>Strep-Tactin®-HRP</t> conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).
    Pbs Solution, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs solution/product/GE Healthcare
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pbs solution - by Bioz Stars, 2020-08
    92/100 stars
    		  Buy from Supplier
    buffer solution pbs  (GE Healthcare)
    93
    GE Healthcare buffer solution pbs
    Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a <t>Strep-Tactin®-HRP</t> conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).
    Buffer Solution Pbs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/buffer solution pbs/product/GE Healthcare
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    buffer solution pbs - by Bioz Stars, 2020-08
    93/100 stars
    		  Buy from Supplier

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    Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a Strep-Tactin®-HRP conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).

    Journal: PLoS ONE

    Article Title: Maltose-Binding Protein (MBP), a Secretion-Enhancing Tag for Mammalian Protein Expression Systems

    doi: 10.1371/journal.pone.0152386

    Figure Lengend Snippet: Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a Strep-Tactin®-HRP conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).

    Article Snippet: The membrane was blocked with 1 x PBS, 0.5% Tween 20 supplemented with 3% BSA for 1 h. Then the block solution was discarded and Strep-Tactin®-HRP detection solution (1:100000 dilution in 1 x PBS, 0.1% Tween 20) was directly added to the membrane for 1 h. The membrane was then washed twice with 1 x PBS, 0.1% Tween-20 and twice with 1 x PBS for 1 min before the membrane was developed using ECL™ (GE Healthcare Europe GmbH, Freiburg, Germany, Cat. no.: RPN2232).

    Techniques: Expressing, Transfection, Sequencing, Fluorescence, Microscopy, Western Blot