phosphate buffered saline pbs solution  (GE Healthcare)

 
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    Name:
    PBS without calcium magnesium
    Description:

    Catalog Number:
    sh30256.01
    Price:
    18.92 USD
    Size:
    500 mL
    Buy from Supplier


    Structured Review

    GE Healthcare phosphate buffered saline pbs solution

    https://www.bioz.com/result/phosphate buffered saline pbs solution/product/GE Healthcare
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    phosphate buffered saline pbs solution - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: Monitoring Chemokine Receptor Trafficking by Confocal Immunofluorescence Microscopy
    Article Snippet: .. HeLa cells; can be obtained from American Type Culture Collection (ATCC) DMEM (Hyclone #SH3002201) FBS (Fisher Scientific #03600511) 0.05% Trypsin–EDTA 1× (Life Technologies #25300054) Phosphate-buffered saline (PBS) 1× (Hyclone #SH30256.01) Mounting medium with DAPI (Vector Laboratories #H1200; Life Technologies Prolong Gold # ) Microcentrifuge tubes (Dot Scientific #010) Coverslips, No. 1.5 (Dot Scientific #MC22-15) Poly- l -lysine (Sigma #P1399) Formaldehyde (Sigma #X100) Triton X-100 (Sigma #F1635) Slides (Fisher Scientific #125442) 10 cm dishes (Falcon #353003) 6-well dishes (Falcon #353046) Primary antibodies against CXCR4 (BD Transduction Laboratories #BDB551852); LAMP2 (Developmental Studies Hybridoma Bank #H4B4), EEA1 (BD Transduction Laboratories #BDB610456), β-arrestin1 (kindly provided by Jeffrey L. Benovic, Thomas Jefferson University, Philadelphia, PA) Secondary antibodies (Molecular Probes); goat anti-rat IgG (H+L), F(ab′)2 Fragment (Alexa Fluor 649) #A21094; goat anti-mouse IgG (H+L), F(ab′)2 Fragment (Alexa Fluor 488 conjugate) # siRNA (Dharmacon) against Luciferase (P-002099), β-arrestin1 (siGenome SMARTpool #M-011971) Lipofectamine 3000 Transfection Reagent (Life Technologies #L3000008) Opti-MEM (Life Technologies #31985062) Hemacytometer (Fisher Scientific #0267154) Tissue paper (Fisher Scientific #06666A) Tweezers Lens paper Whatman filter paper Parafilm Confocal microscope equipped with 60× objective ..

    Luciferase:

    Article Title: Monitoring Chemokine Receptor Trafficking by Confocal Immunofluorescence Microscopy
    Article Snippet: .. HeLa cells; can be obtained from American Type Culture Collection (ATCC) DMEM (Hyclone #SH3002201) FBS (Fisher Scientific #03600511) 0.05% Trypsin–EDTA 1× (Life Technologies #25300054) Phosphate-buffered saline (PBS) 1× (Hyclone #SH30256.01) Mounting medium with DAPI (Vector Laboratories #H1200; Life Technologies Prolong Gold # ) Microcentrifuge tubes (Dot Scientific #010) Coverslips, No. 1.5 (Dot Scientific #MC22-15) Poly- l -lysine (Sigma #P1399) Formaldehyde (Sigma #X100) Triton X-100 (Sigma #F1635) Slides (Fisher Scientific #125442) 10 cm dishes (Falcon #353003) 6-well dishes (Falcon #353046) Primary antibodies against CXCR4 (BD Transduction Laboratories #BDB551852); LAMP2 (Developmental Studies Hybridoma Bank #H4B4), EEA1 (BD Transduction Laboratories #BDB610456), β-arrestin1 (kindly provided by Jeffrey L. Benovic, Thomas Jefferson University, Philadelphia, PA) Secondary antibodies (Molecular Probes); goat anti-rat IgG (H+L), F(ab′)2 Fragment (Alexa Fluor 649) #A21094; goat anti-mouse IgG (H+L), F(ab′)2 Fragment (Alexa Fluor 488 conjugate) # siRNA (Dharmacon) against Luciferase (P-002099), β-arrestin1 (siGenome SMARTpool #M-011971) Lipofectamine 3000 Transfection Reagent (Life Technologies #L3000008) Opti-MEM (Life Technologies #31985062) Hemacytometer (Fisher Scientific #0267154) Tissue paper (Fisher Scientific #06666A) Tweezers Lens paper Whatman filter paper Parafilm Confocal microscope equipped with 60× objective ..

    Protease Inhibitor:

    Article Title: Melatonin Preserves Blood-Brain Barrier Integrity and Permeability via Matrix Metalloproteinase-9 Inhibition
    Article Snippet: .. Rabbit anti ZO-1 (Cat # 617300), mouse anti ZO-1 (Cat # 339100), 0.25% Trypsin (1X), Opti-MEM (1X)/reduced serum medium, Dulbecco's modified Eagle’s medium (DMEM; with high glucose, HEPES, no phenol red (1X)), NuPAGE Novex® 10% Bis-Tris protein gels, NuPAGE® MOPS SDS Running Buffer, NuPAGE® Transfer Buffer, HyClone Dulbecco's phosphate buffered saline (PBS, without calcium, magnesium, or phenol red), TRIzol® Reagent, SuperScript® IV First-Strand Synthesis System, Halt® Protease Inhibitor Cocktail (100X), Pierce™ ECL Western Blotting substrate and rhodamine phalloidin were purchased from Thermo Fisher Scientific (Carlsbad, CA). .. Anti-MMP-9 antibody (cat # ab-3) was obtained from EMD Millipore (Billerica, MA).

    Article Title: Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma
    Article Snippet: .. Tissue dissociation and cell purification Tissues were transported in RPMI 1640 (Gibco, Cat. no. 11875–093) with 1 mM protease inhibitor (Solarbio, Cat. no. P6730) on ice to preserve viability, washed 2–3 times with phosphate buffered saline (PBS; Hyclone, Cat. no. SH30256.01), then minced on ice. .. We used dissociation enzyme cocktail 1 mg/ml Type VIII Collagenase (Sigma-Aldrich, Cat. no. C2139), 2 mg/ml Dispase II (Sigma-Aldrich, Cat. no.4942078001), 1 mg/ml Trypsin Inhibitor (Sigma-Aldrich, Cat. no. T6522) and 1 unit/ml DNase I (NEB, Cat. no. M0303S) dissolved in PBS with 5% Fetal Bovine Serum (FBS; Gibco, Cat. no. 16000–044) to digest the tissues.

    Microscopy:

    Article Title: Monitoring Chemokine Receptor Trafficking by Confocal Immunofluorescence Microscopy
    Article Snippet: .. HeLa cells; can be obtained from American Type Culture Collection (ATCC) DMEM (Hyclone #SH3002201) FBS (Fisher Scientific #03600511) 0.05% Trypsin–EDTA 1× (Life Technologies #25300054) Phosphate-buffered saline (PBS) 1× (Hyclone #SH30256.01) Mounting medium with DAPI (Vector Laboratories #H1200; Life Technologies Prolong Gold # ) Microcentrifuge tubes (Dot Scientific #010) Coverslips, No. 1.5 (Dot Scientific #MC22-15) Poly- l -lysine (Sigma #P1399) Formaldehyde (Sigma #X100) Triton X-100 (Sigma #F1635) Slides (Fisher Scientific #125442) 10 cm dishes (Falcon #353003) 6-well dishes (Falcon #353046) Primary antibodies against CXCR4 (BD Transduction Laboratories #BDB551852); LAMP2 (Developmental Studies Hybridoma Bank #H4B4), EEA1 (BD Transduction Laboratories #BDB610456), β-arrestin1 (kindly provided by Jeffrey L. Benovic, Thomas Jefferson University, Philadelphia, PA) Secondary antibodies (Molecular Probes); goat anti-rat IgG (H+L), F(ab′)2 Fragment (Alexa Fluor 649) #A21094; goat anti-mouse IgG (H+L), F(ab′)2 Fragment (Alexa Fluor 488 conjugate) # siRNA (Dharmacon) against Luciferase (P-002099), β-arrestin1 (siGenome SMARTpool #M-011971) Lipofectamine 3000 Transfection Reagent (Life Technologies #L3000008) Opti-MEM (Life Technologies #31985062) Hemacytometer (Fisher Scientific #0267154) Tissue paper (Fisher Scientific #06666A) Tweezers Lens paper Whatman filter paper Parafilm Confocal microscope equipped with 60× objective ..

    Purification:

    Article Title: Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma
    Article Snippet: .. Tissue dissociation and cell purification Tissues were transported in RPMI 1640 (Gibco, Cat. no. 11875–093) with 1 mM protease inhibitor (Solarbio, Cat. no. P6730) on ice to preserve viability, washed 2–3 times with phosphate buffered saline (PBS; Hyclone, Cat. no. SH30256.01), then minced on ice. .. We used dissociation enzyme cocktail 1 mg/ml Type VIII Collagenase (Sigma-Aldrich, Cat. no. C2139), 2 mg/ml Dispase II (Sigma-Aldrich, Cat. no.4942078001), 1 mg/ml Trypsin Inhibitor (Sigma-Aldrich, Cat. no. T6522) and 1 unit/ml DNase I (NEB, Cat. no. M0303S) dissolved in PBS with 5% Fetal Bovine Serum (FBS; Gibco, Cat. no. 16000–044) to digest the tissues.

    Concentration Assay:

    Article Title: A Synthetic Tetramer of Galectin-1 and Galectin-3 Amplifies Pro-apoptotic Signaling by Integrating the Activity of Both Galectins
    Article Snippet: .. For all apoptosis experiments, 100 μL of cells were aliquoted at 200,000 cells into round-bottom 12 × 75 mm culture test tubes (14-956-3D, ThermoFisher) and incubated with 100 μL of sterile 1x PBS (Hyclone™ SH30256) alone (i.e., untreated), G1, G3, G1 + G3, or G1/G3 Zipper in sterile 1x PBS (final galectin concentration depending on assay) in the presence or absence of 100 μM caspase-8 inhibitor Z-IETD-FMK (FMK007, R & D Systems), caspase-3/7 inhibitor I (218826, MilliporeSigma), ERK inhibitor U0126 (662005, MilliporeSigma), or JNK inhibitor II SP600125 (420119, MilliporeSigma) for 4 or 24 h at 37°C, 5% CO2 . .. Note, inhibitors were dissolved in American Chemical Society grade dimethyl sulfoxide (DMSO) and an equivalent amount of DMSO (1 μL or 0.5% final concentration) was added to all groups not receiving inhibitors as vehicle control.

    Article Title: Host Immune Response Triggered by Graphene Quantum-Dot-Mediated Photodynamic Therapy for Oral Squamous Cell Carcinoma
    Article Snippet: .. For observation of the cellular uptake of GQD-PEG, SCC 9 and human oral keratinocytes (HOK) were seeded in confocal Petri dishes (15 mm) at a density of 104 cells/mL and cultured for 24 h. Cells were then incubated with GQD-PEG or pristine GQD at a concentration of 50 μg/mL for 6 h and washed 3 times with phosphate-buffered saline (PBS; Hyclone, Logan, UT, USA). .. For the tracking of intracellular distribution, cells were stained with 4′,6-diamidino-2-phenylindole (DAPI) and observed by confocal laser scanning microscopy (CLSM; Zeiss, Jena, Germany).

    Incubation:

    Article Title: A Synthetic Tetramer of Galectin-1 and Galectin-3 Amplifies Pro-apoptotic Signaling by Integrating the Activity of Both Galectins
    Article Snippet: .. For all apoptosis experiments, 100 μL of cells were aliquoted at 200,000 cells into round-bottom 12 × 75 mm culture test tubes (14-956-3D, ThermoFisher) and incubated with 100 μL of sterile 1x PBS (Hyclone™ SH30256) alone (i.e., untreated), G1, G3, G1 + G3, or G1/G3 Zipper in sterile 1x PBS (final galectin concentration depending on assay) in the presence or absence of 100 μM caspase-8 inhibitor Z-IETD-FMK (FMK007, R & D Systems), caspase-3/7 inhibitor I (218826, MilliporeSigma), ERK inhibitor U0126 (662005, MilliporeSigma), or JNK inhibitor II SP600125 (420119, MilliporeSigma) for 4 or 24 h at 37°C, 5% CO2 . .. Note, inhibitors were dissolved in American Chemical Society grade dimethyl sulfoxide (DMSO) and an equivalent amount of DMSO (1 μL or 0.5% final concentration) was added to all groups not receiving inhibitors as vehicle control.

    Article Title: Host Immune Response Triggered by Graphene Quantum-Dot-Mediated Photodynamic Therapy for Oral Squamous Cell Carcinoma
    Article Snippet: .. For observation of the cellular uptake of GQD-PEG, SCC 9 and human oral keratinocytes (HOK) were seeded in confocal Petri dishes (15 mm) at a density of 104 cells/mL and cultured for 24 h. Cells were then incubated with GQD-PEG or pristine GQD at a concentration of 50 μg/mL for 6 h and washed 3 times with phosphate-buffered saline (PBS; Hyclone, Logan, UT, USA). .. For the tracking of intracellular distribution, cells were stained with 4′,6-diamidino-2-phenylindole (DAPI) and observed by confocal laser scanning microscopy (CLSM; Zeiss, Jena, Germany).

    Article Title: Exosomes derived from neural progenitor cells preserve photoreceptors during retinal degeneration by inactivating microglia
    Article Snippet: .. BV2 cells were seeded in the transwell chambers (Cat. No. MCRP24H48, Millipore) at a density of 2 × 104 cells/chamber or 1 × 105 cells/plate for 6 h before treatment with lipopolysaccharides (LPS, 1ug/mL) (Cat. No. L4516, Sigma) for 24 h. For further incubation with mNPC-exos, BV2 chambers were washed with warmed PBS (Cat. No. SH30256.01, Hyclone), moved into 661 W wells, added fresh mNPC-exos in the upper chamber and co-cultured till 48 h for further assay. .. Human NPC were isolated from human embryonic retina which was provided by the embryonic tissue bank of the Department of Obstetrics in Southwest Hospital [ ].

    Cell Culture:

    Article Title: Host Immune Response Triggered by Graphene Quantum-Dot-Mediated Photodynamic Therapy for Oral Squamous Cell Carcinoma
    Article Snippet: .. For observation of the cellular uptake of GQD-PEG, SCC 9 and human oral keratinocytes (HOK) were seeded in confocal Petri dishes (15 mm) at a density of 104 cells/mL and cultured for 24 h. Cells were then incubated with GQD-PEG or pristine GQD at a concentration of 50 μg/mL for 6 h and washed 3 times with phosphate-buffered saline (PBS; Hyclone, Logan, UT, USA). .. For the tracking of intracellular distribution, cells were stained with 4′,6-diamidino-2-phenylindole (DAPI) and observed by confocal laser scanning microscopy (CLSM; Zeiss, Jena, Germany).

    Modification:

    Article Title: Melatonin Preserves Blood-Brain Barrier Integrity and Permeability via Matrix Metalloproteinase-9 Inhibition
    Article Snippet: .. Rabbit anti ZO-1 (Cat # 617300), mouse anti ZO-1 (Cat # 339100), 0.25% Trypsin (1X), Opti-MEM (1X)/reduced serum medium, Dulbecco's modified Eagle’s medium (DMEM; with high glucose, HEPES, no phenol red (1X)), NuPAGE Novex® 10% Bis-Tris protein gels, NuPAGE® MOPS SDS Running Buffer, NuPAGE® Transfer Buffer, HyClone Dulbecco's phosphate buffered saline (PBS, without calcium, magnesium, or phenol red), TRIzol® Reagent, SuperScript® IV First-Strand Synthesis System, Halt® Protease Inhibitor Cocktail (100X), Pierce™ ECL Western Blotting substrate and rhodamine phalloidin were purchased from Thermo Fisher Scientific (Carlsbad, CA). .. Anti-MMP-9 antibody (cat # ab-3) was obtained from EMD Millipore (Billerica, MA).

    Western Blot:

    Article Title: Melatonin Preserves Blood-Brain Barrier Integrity and Permeability via Matrix Metalloproteinase-9 Inhibition
    Article Snippet: .. Rabbit anti ZO-1 (Cat # 617300), mouse anti ZO-1 (Cat # 339100), 0.25% Trypsin (1X), Opti-MEM (1X)/reduced serum medium, Dulbecco's modified Eagle’s medium (DMEM; with high glucose, HEPES, no phenol red (1X)), NuPAGE Novex® 10% Bis-Tris protein gels, NuPAGE® MOPS SDS Running Buffer, NuPAGE® Transfer Buffer, HyClone Dulbecco's phosphate buffered saline (PBS, without calcium, magnesium, or phenol red), TRIzol® Reagent, SuperScript® IV First-Strand Synthesis System, Halt® Protease Inhibitor Cocktail (100X), Pierce™ ECL Western Blotting substrate and rhodamine phalloidin were purchased from Thermo Fisher Scientific (Carlsbad, CA). .. Anti-MMP-9 antibody (cat # ab-3) was obtained from EMD Millipore (Billerica, MA).

    Immunofluorescence:

    Article Title: In vitro model of the glial scar
    Article Snippet: .. 2.5 Immunofluorescence StainingThe embedded astrocytes were fixed with 4% paraformaldehyde for an hour and washed with 1× PBS (phosphate-buffered saline, HyClone, SH30256.01) 6 times at 10 min intervals. .. Then, the method of freezing slice was used to obtain 40 µm thickness slices for the immunofluorescence staining of the embedded cells.

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    GE Healthcare strep tactin hrp detection solution
    Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a <t>Strep-Tactin®-HRP</t> conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).
    Strep Tactin Hrp Detection Solution, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/strep tactin hrp detection solution/product/GE Healthcare
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    strep tactin hrp detection solution - by Bioz Stars, 2021-01
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    99
    GE Healthcare phosphate buffered saline pbs solution
    Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a <t>Strep-Tactin®-HRP</t> conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).
    Phosphate Buffered Saline Pbs Solution, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffered saline pbs solution/product/GE Healthcare
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    phosphate buffered saline pbs solution - by Bioz Stars, 2021-01
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    92
    GE Healthcare pbs solution
    Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a <t>Strep-Tactin®-HRP</t> conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).
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    Average 92 stars, based on 3 article reviews
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    99
    GE Healthcare bovine serum albumin solution
    Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a <t>Strep-Tactin®-HRP</t> conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).
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    Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a Strep-Tactin®-HRP conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).

    Journal: PLoS ONE

    Article Title: Maltose-Binding Protein (MBP), a Secretion-Enhancing Tag for Mammalian Protein Expression Systems

    doi: 10.1371/journal.pone.0152386

    Figure Lengend Snippet: Enhanced expression of eGFP. HEK293 cells were transiently transfected with eGFP alone (basic) or fused to different expression tags. (A) All proteins contained a signal peptide sequence for secretion. The eGFP signals were visualized by fluorescence microscopy. (B) To quantify the secreted eGFP, the supernatants from the HEK293 cells were excited with a laser at 488 nm and the emission signal was detected at 509 nm (top). Densitometric analysis of secreted eGFP from HEK293 cells by western blot analysis using a Strep-Tactin®-HRP conjugate to detect Strep II tagged eGFP fusion proteins (bottom). (C) Without signal peptide, eGFP was transiently expressed intracellularly with or without fused MBP. eGFP signals were visualized by fluorescence microscopy. (a.u.: arbitrary unit).

    Article Snippet: The membrane was blocked with 1 x PBS, 0.5% Tween 20 supplemented with 3% BSA for 1 h. Then the block solution was discarded and Strep-Tactin®-HRP detection solution (1:100000 dilution in 1 x PBS, 0.1% Tween 20) was directly added to the membrane for 1 h. The membrane was then washed twice with 1 x PBS, 0.1% Tween-20 and twice with 1 x PBS for 1 min before the membrane was developed using ECL™ (GE Healthcare Europe GmbH, Freiburg, Germany, Cat. no.: RPN2232).

    Techniques: Expressing, Transfection, Sequencing, Fluorescence, Microscopy, Western Blot