Flow Cytometry:Article Title: Influence of buoyancy-driven flow on mass transfer in a two-stream microfluidic channel: Introduction of cryoprotective agents into cell suspensions
Article Snippet: .. Two streams flow through the device: a cell stream that consists of phosphate buffered saline (PBS) solution (Fischer Scientific, Walkersville, MD) with or without Jurkat cells (ATCC TIB-1522, Manassas, VA), and the donor stream consisting of PBS with DMSO (Sigma, St. Louis, MO). ..
Blocking Assay:Article Title: Complement in Human Pre-implantation Embryos: Attack and Defense
Article Snippet: .. Confocal Microscopy Embryos were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) solution for 15 min at room temperature, washed with PBS and permeabilized using 0.5% Triton® X-100 (Fisher Scientific, Geel, Belgium) in PBS for 15 min, and blocked for unspecific staining using Ultra Vision Protein Block (Thermo Scientific, MI) for 8–10 min, all at room temperature. .. Primary antibodies, mouse anti-CD46 (GeneTex Inc., Irvine, California, US; 1:100), mouse anti-CD55 (IBGRL Research Products, Bristol, UK; 1:100), mouse anti-CD59 (IBGRL Research Products, Bristol, UK; 1:100), goat anti-factor H (Calbiochem, La Jolla, California; 1:100), mouse anti-C4bp (Quidel, San Diego, California; 1:100), rabbit anti-C1q (DAKO Denmark A/S, Glostrup, Denmark; 1:100), goat anti-C5 (Cappel, Organon Teknika Corp., West Chester, Pennsylvania; 1:300), rabbit anti-C3d (DAKO Denmark A/S, Glostrup, Denmark; 1:100), rabbit anti-C3c (DAKO Denmark A/S, Glostrup, Denmark; 1:100) were diluted in PBS + 0.1% Tween20 (Fisher Scientific, Geel, Belgium) and incubated over night at +4°C.
Transgenic Assay:Article Title: Myogenic Conversion and Transcriptional Profiling of Embryonic Blastomeres in C. elegans
Article Snippet: .. Wild-type, transgenic, or RNAi treated gravid adult worms are transferred into 30 µl of phosphate buffered saline (PBS) solution in one of two wells of a hanging drop glass slide (e.g. Fisher Scientific, cat# 12-565B). .. A typical target number of embryos to collect is 80 at the 1- to 4-cell stage, thus 60–100 gravid adults are picked for dissection.
other:Article Title: Direct patterning of gold nanoparticles using flexographic printing for biosensing applications
Article Snippet: Paraffin wax and Phosphate buffered saline (PBS) solution with pH 7.4 were purchased from Fisher Scientific (Loughborough, UK).
Article Title: Sodium Hypochlorite Treatment and Nitinol Performance for Medical Devices
Article Snippet: Briefly, specimens were placed into a deaerated phosphate-buffered saline (PBS) solution (Fisher Scientific) at 37 °C and allowed to equilibrate for 60 min while the open-circuit potential was recorded.
Article Title: Synthesis and characterization of CaSr-Metal Organic Frameworks for biodegradable orthopedic applications
Article Snippet: Phosphate Buffered Saline (PBS) solution without Mg and Ca (Fischer Scientific, Pittsburgh, PA) was added to the tube in 1 ml quantities.
Confocal Microscopy:Article Title: Complement in Human Pre-implantation Embryos: Attack and Defense
Article Snippet: .. Confocal Microscopy Embryos were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) solution for 15 min at room temperature, washed with PBS and permeabilized using 0.5% Triton® X-100 (Fisher Scientific, Geel, Belgium) in PBS for 15 min, and blocked for unspecific staining using Ultra Vision Protein Block (Thermo Scientific, MI) for 8–10 min, all at room temperature. .. Primary antibodies, mouse anti-CD46 (GeneTex Inc., Irvine, California, US; 1:100), mouse anti-CD55 (IBGRL Research Products, Bristol, UK; 1:100), mouse anti-CD59 (IBGRL Research Products, Bristol, UK; 1:100), goat anti-factor H (Calbiochem, La Jolla, California; 1:100), mouse anti-C4bp (Quidel, San Diego, California; 1:100), rabbit anti-C1q (DAKO Denmark A/S, Glostrup, Denmark; 1:100), goat anti-C5 (Cappel, Organon Teknika Corp., West Chester, Pennsylvania; 1:300), rabbit anti-C3d (DAKO Denmark A/S, Glostrup, Denmark; 1:100), rabbit anti-C3c (DAKO Denmark A/S, Glostrup, Denmark; 1:100) were diluted in PBS + 0.1% Tween20 (Fisher Scientific, Geel, Belgium) and incubated over night at +4°C.
Staining:Article Title: Complement in Human Pre-implantation Embryos: Attack and Defense
Article Snippet: .. Confocal Microscopy Embryos were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) solution for 15 min at room temperature, washed with PBS and permeabilized using 0.5% Triton® X-100 (Fisher Scientific, Geel, Belgium) in PBS for 15 min, and blocked for unspecific staining using Ultra Vision Protein Block (Thermo Scientific, MI) for 8–10 min, all at room temperature. .. Primary antibodies, mouse anti-CD46 (GeneTex Inc., Irvine, California, US; 1:100), mouse anti-CD55 (IBGRL Research Products, Bristol, UK; 1:100), mouse anti-CD59 (IBGRL Research Products, Bristol, UK; 1:100), goat anti-factor H (Calbiochem, La Jolla, California; 1:100), mouse anti-C4bp (Quidel, San Diego, California; 1:100), rabbit anti-C1q (DAKO Denmark A/S, Glostrup, Denmark; 1:100), goat anti-C5 (Cappel, Organon Teknika Corp., West Chester, Pennsylvania; 1:300), rabbit anti-C3d (DAKO Denmark A/S, Glostrup, Denmark; 1:100), rabbit anti-C3c (DAKO Denmark A/S, Glostrup, Denmark; 1:100) were diluted in PBS + 0.1% Tween20 (Fisher Scientific, Geel, Belgium) and incubated over night at +4°C.
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