Structured Review

Fisher Scientific phosphate buffered saline pbs solution
Phosphate Buffered Saline Pbs Solution, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphate buffered saline pbs solution/product/Fisher Scientific
Average 93 stars, based on 12 article reviews
Price from $9.99 to $1999.99
phosphate buffered saline pbs solution - by Bioz Stars, 2020-08
93/100 stars

Images

Related Articles

Flow Cytometry:

Article Title: Influence of buoyancy-driven flow on mass transfer in a two-stream microfluidic channel: Introduction of cryoprotective agents into cell suspensions
Article Snippet: .. Two streams flow through the device: a cell stream that consists of phosphate buffered saline (PBS) solution (Fischer Scientific, Walkersville, MD) with or without Jurkat cells (ATCC TIB-1522, Manassas, VA), and the donor stream consisting of PBS with DMSO (Sigma, St. Louis, MO). ..

Blocking Assay:

Article Title: Complement in Human Pre-implantation Embryos: Attack and Defense
Article Snippet: .. Confocal Microscopy Embryos were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) solution for 15 min at room temperature, washed with PBS and permeabilized using 0.5% Triton® X-100 (Fisher Scientific, Geel, Belgium) in PBS for 15 min, and blocked for unspecific staining using Ultra Vision Protein Block (Thermo Scientific, MI) for 8–10 min, all at room temperature. .. Primary antibodies, mouse anti-CD46 (GeneTex Inc., Irvine, California, US; 1:100), mouse anti-CD55 (IBGRL Research Products, Bristol, UK; 1:100), mouse anti-CD59 (IBGRL Research Products, Bristol, UK; 1:100), goat anti-factor H (Calbiochem, La Jolla, California; 1:100), mouse anti-C4bp (Quidel, San Diego, California; 1:100), rabbit anti-C1q (DAKO Denmark A/S, Glostrup, Denmark; 1:100), goat anti-C5 (Cappel, Organon Teknika Corp., West Chester, Pennsylvania; 1:300), rabbit anti-C3d (DAKO Denmark A/S, Glostrup, Denmark; 1:100), rabbit anti-C3c (DAKO Denmark A/S, Glostrup, Denmark; 1:100) were diluted in PBS + 0.1% Tween20 (Fisher Scientific, Geel, Belgium) and incubated over night at +4°C.

Transgenic Assay:

Article Title: Myogenic Conversion and Transcriptional Profiling of Embryonic Blastomeres in C. elegans
Article Snippet: .. Wild-type, transgenic, or RNAi treated gravid adult worms are transferred into 30 µl of phosphate buffered saline (PBS) solution in one of two wells of a hanging drop glass slide (e.g. Fisher Scientific, cat# 12-565B). .. A typical target number of embryos to collect is 80 at the 1- to 4-cell stage, thus 60–100 gravid adults are picked for dissection.

other:

Article Title: Direct patterning of gold nanoparticles using flexographic printing for biosensing applications
Article Snippet: Paraffin wax and Phosphate buffered saline (PBS) solution with pH 7.4 were purchased from Fisher Scientific (Loughborough, UK).

Article Title: Sodium Hypochlorite Treatment and Nitinol Performance for Medical Devices
Article Snippet: Briefly, specimens were placed into a deaerated phosphate-buffered saline (PBS) solution (Fisher Scientific) at 37 °C and allowed to equilibrate for 60 min while the open-circuit potential was recorded.

Article Title: Synthesis and characterization of CaSr-Metal Organic Frameworks for biodegradable orthopedic applications
Article Snippet: Phosphate Buffered Saline (PBS) solution without Mg and Ca (Fischer Scientific, Pittsburgh, PA) was added to the tube in 1 ml quantities.

Confocal Microscopy:

Article Title: Complement in Human Pre-implantation Embryos: Attack and Defense
Article Snippet: .. Confocal Microscopy Embryos were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) solution for 15 min at room temperature, washed with PBS and permeabilized using 0.5% Triton® X-100 (Fisher Scientific, Geel, Belgium) in PBS for 15 min, and blocked for unspecific staining using Ultra Vision Protein Block (Thermo Scientific, MI) for 8–10 min, all at room temperature. .. Primary antibodies, mouse anti-CD46 (GeneTex Inc., Irvine, California, US; 1:100), mouse anti-CD55 (IBGRL Research Products, Bristol, UK; 1:100), mouse anti-CD59 (IBGRL Research Products, Bristol, UK; 1:100), goat anti-factor H (Calbiochem, La Jolla, California; 1:100), mouse anti-C4bp (Quidel, San Diego, California; 1:100), rabbit anti-C1q (DAKO Denmark A/S, Glostrup, Denmark; 1:100), goat anti-C5 (Cappel, Organon Teknika Corp., West Chester, Pennsylvania; 1:300), rabbit anti-C3d (DAKO Denmark A/S, Glostrup, Denmark; 1:100), rabbit anti-C3c (DAKO Denmark A/S, Glostrup, Denmark; 1:100) were diluted in PBS + 0.1% Tween20 (Fisher Scientific, Geel, Belgium) and incubated over night at +4°C.

Staining:

Article Title: Complement in Human Pre-implantation Embryos: Attack and Defense
Article Snippet: .. Confocal Microscopy Embryos were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) solution for 15 min at room temperature, washed with PBS and permeabilized using 0.5% Triton® X-100 (Fisher Scientific, Geel, Belgium) in PBS for 15 min, and blocked for unspecific staining using Ultra Vision Protein Block (Thermo Scientific, MI) for 8–10 min, all at room temperature. .. Primary antibodies, mouse anti-CD46 (GeneTex Inc., Irvine, California, US; 1:100), mouse anti-CD55 (IBGRL Research Products, Bristol, UK; 1:100), mouse anti-CD59 (IBGRL Research Products, Bristol, UK; 1:100), goat anti-factor H (Calbiochem, La Jolla, California; 1:100), mouse anti-C4bp (Quidel, San Diego, California; 1:100), rabbit anti-C1q (DAKO Denmark A/S, Glostrup, Denmark; 1:100), goat anti-C5 (Cappel, Organon Teknika Corp., West Chester, Pennsylvania; 1:300), rabbit anti-C3d (DAKO Denmark A/S, Glostrup, Denmark; 1:100), rabbit anti-C3c (DAKO Denmark A/S, Glostrup, Denmark; 1:100) were diluted in PBS + 0.1% Tween20 (Fisher Scientific, Geel, Belgium) and incubated over night at +4°C.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 89
    Fisher Scientific dox solution
    Characterization of <t>FA-HP-β-CD-PEI/DOX/siRNA</t> FAM nanocomplexes by gel electrophoresis assay. Notes: The agarose gel electrophoresis showed the behavior of nanocomplexes with various N/P ratios (0, 1, 2, 4, 8, 16, and 32). When the N/P ratio was 16:1, the siRNA was retarded completely, and this N/P ratio was used in the following experiments. siRNA FAM Ex =488 nm, Em =535 nm, and the concentration was 100 nM. The gel was 1.5% and run for 10 minutes at 150 V. Abbreviations: DOX, doxorubicin; siRNA FAM , small interfering RNA link with FAM green fluorescent dye; FA, folic acid; HP-β-CD, hydroxypropyl-β-cyclodextrin; PEI, polyethylenimine; Ex, excitation; Em, emission.
    Dox Solution, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dox solution/product/Fisher Scientific
    Average 89 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    dox solution - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    92
    Fisher Scientific cold pbs solution
    Characterization of <t>FA-HP-β-CD-PEI/DOX/siRNA</t> FAM nanocomplexes by gel electrophoresis assay. Notes: The agarose gel electrophoresis showed the behavior of nanocomplexes with various N/P ratios (0, 1, 2, 4, 8, 16, and 32). When the N/P ratio was 16:1, the siRNA was retarded completely, and this N/P ratio was used in the following experiments. siRNA FAM Ex =488 nm, Em =535 nm, and the concentration was 100 nM. The gel was 1.5% and run for 10 minutes at 150 V. Abbreviations: DOX, doxorubicin; siRNA FAM , small interfering RNA link with FAM green fluorescent dye; FA, folic acid; HP-β-CD, hydroxypropyl-β-cyclodextrin; PEI, polyethylenimine; Ex, excitation; Em, emission.
    Cold Pbs Solution, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cold pbs solution/product/Fisher Scientific
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    cold pbs solution - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    88
    Fisher Scientific pbs pi
    Characterization of <t>FA-HP-β-CD-PEI/DOX/siRNA</t> FAM nanocomplexes by gel electrophoresis assay. Notes: The agarose gel electrophoresis showed the behavior of nanocomplexes with various N/P ratios (0, 1, 2, 4, 8, 16, and 32). When the N/P ratio was 16:1, the siRNA was retarded completely, and this N/P ratio was used in the following experiments. siRNA FAM Ex =488 nm, Em =535 nm, and the concentration was 100 nM. The gel was 1.5% and run for 10 minutes at 150 V. Abbreviations: DOX, doxorubicin; siRNA FAM , small interfering RNA link with FAM green fluorescent dye; FA, folic acid; HP-β-CD, hydroxypropyl-β-cyclodextrin; PEI, polyethylenimine; Ex, excitation; Em, emission.
    Pbs Pi, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs pi/product/Fisher Scientific
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pbs pi - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    Image Search Results


    Characterization of FA-HP-β-CD-PEI/DOX/siRNA FAM nanocomplexes by gel electrophoresis assay. Notes: The agarose gel electrophoresis showed the behavior of nanocomplexes with various N/P ratios (0, 1, 2, 4, 8, 16, and 32). When the N/P ratio was 16:1, the siRNA was retarded completely, and this N/P ratio was used in the following experiments. siRNA FAM Ex =488 nm, Em =535 nm, and the concentration was 100 nM. The gel was 1.5% and run for 10 minutes at 150 V. Abbreviations: DOX, doxorubicin; siRNA FAM , small interfering RNA link with FAM green fluorescent dye; FA, folic acid; HP-β-CD, hydroxypropyl-β-cyclodextrin; PEI, polyethylenimine; Ex, excitation; Em, emission.

    Journal: International Journal of Nanomedicine

    Article Title: Reversal of multidrug resistance in MCF-7/Adr cells by codelivery of doxorubicin and BCL2 siRNA using a folic acid-conjugated polyethylenimine hydroxypropyl-β-cyclodextrin nanocarrier

    doi: 10.2147/IJN.S67146

    Figure Lengend Snippet: Characterization of FA-HP-β-CD-PEI/DOX/siRNA FAM nanocomplexes by gel electrophoresis assay. Notes: The agarose gel electrophoresis showed the behavior of nanocomplexes with various N/P ratios (0, 1, 2, 4, 8, 16, and 32). When the N/P ratio was 16:1, the siRNA was retarded completely, and this N/P ratio was used in the following experiments. siRNA FAM Ex =488 nm, Em =535 nm, and the concentration was 100 nM. The gel was 1.5% and run for 10 minutes at 150 V. Abbreviations: DOX, doxorubicin; siRNA FAM , small interfering RNA link with FAM green fluorescent dye; FA, folic acid; HP-β-CD, hydroxypropyl-β-cyclodextrin; PEI, polyethylenimine; Ex, excitation; Em, emission.

    Article Snippet: Preparation and characterization of FA-HP-β-CD-PEI/DOX nanocomplexes Five milligrams of FA-HP-β-CD-PEI was mixed in a 5 mL DOX solution (phosphate-buffered saline [PBS], 0.5 mg/mL), followed by ultrasonic agitation in a type 60 Sonic Dismembrator (Fisher Scientific, Waltham, MA, USA) for 24 hours.

    Techniques: Nucleic Acid Electrophoresis, Agarose Gel Electrophoresis, Concentration Assay, Small Interfering RNA

    Cell viability of drug-loaded nanocomplexes (HP-β-CD-PEI/DOX, HP-β-CD-PEI/siRNA, FA-HP-β-CD-PEI/DOX, FA-HP-β-CD-PEI/siRNA, HP-β-CD-PEI/DOX/siRNA, and FA-HP-β-CD-PEI/DOX/siRNA). Notes: Untreated cells and free DOX were controls. Concentrations of DOX and siRNA were 0.5 μg/mL and 100 nM, respectively. Treatment time was 48 hours. Abbreviations: FA, folic acid; HP-β-CD, hydroxypropyl-β-cyclodextrin; PEI, polyethylenimine; DOX, doxorubicin; siRNA, small interfering RNA.

    Journal: International Journal of Nanomedicine

    Article Title: Reversal of multidrug resistance in MCF-7/Adr cells by codelivery of doxorubicin and BCL2 siRNA using a folic acid-conjugated polyethylenimine hydroxypropyl-β-cyclodextrin nanocarrier

    doi: 10.2147/IJN.S67146

    Figure Lengend Snippet: Cell viability of drug-loaded nanocomplexes (HP-β-CD-PEI/DOX, HP-β-CD-PEI/siRNA, FA-HP-β-CD-PEI/DOX, FA-HP-β-CD-PEI/siRNA, HP-β-CD-PEI/DOX/siRNA, and FA-HP-β-CD-PEI/DOX/siRNA). Notes: Untreated cells and free DOX were controls. Concentrations of DOX and siRNA were 0.5 μg/mL and 100 nM, respectively. Treatment time was 48 hours. Abbreviations: FA, folic acid; HP-β-CD, hydroxypropyl-β-cyclodextrin; PEI, polyethylenimine; DOX, doxorubicin; siRNA, small interfering RNA.

    Article Snippet: Preparation and characterization of FA-HP-β-CD-PEI/DOX nanocomplexes Five milligrams of FA-HP-β-CD-PEI was mixed in a 5 mL DOX solution (phosphate-buffered saline [PBS], 0.5 mg/mL), followed by ultrasonic agitation in a type 60 Sonic Dismembrator (Fisher Scientific, Waltham, MA, USA) for 24 hours.

    Techniques: Small Interfering RNA

    The release behavior of the DOX from the DOX-loaded nanocomplexes in different pH conditions (pH 5.0 and pH 7.4). Notes: When the DOX-loaded nanocomplexes were in the normal pH condition (pH 7.4), the DOX released from the nanocomplexes very slowly, and compared to the normal pH condition, the DOX released very quickly at pH 5.0 from the nanocomplexes, showing that the release of the DOX from the nanocarrier can be controlled by pH. DOX concentration of 0.5 μg/mL. Abbreviations: DOX, doxorubicin; siRNA, small interfering RNA; FA, folic acid; HP-β-CD, hydroxypropyl-β-cyclodextrin; PEI, polyethylenimine.

    Journal: International Journal of Nanomedicine

    Article Title: Reversal of multidrug resistance in MCF-7/Adr cells by codelivery of doxorubicin and BCL2 siRNA using a folic acid-conjugated polyethylenimine hydroxypropyl-β-cyclodextrin nanocarrier

    doi: 10.2147/IJN.S67146

    Figure Lengend Snippet: The release behavior of the DOX from the DOX-loaded nanocomplexes in different pH conditions (pH 5.0 and pH 7.4). Notes: When the DOX-loaded nanocomplexes were in the normal pH condition (pH 7.4), the DOX released from the nanocomplexes very slowly, and compared to the normal pH condition, the DOX released very quickly at pH 5.0 from the nanocomplexes, showing that the release of the DOX from the nanocarrier can be controlled by pH. DOX concentration of 0.5 μg/mL. Abbreviations: DOX, doxorubicin; siRNA, small interfering RNA; FA, folic acid; HP-β-CD, hydroxypropyl-β-cyclodextrin; PEI, polyethylenimine.

    Article Snippet: Preparation and characterization of FA-HP-β-CD-PEI/DOX nanocomplexes Five milligrams of FA-HP-β-CD-PEI was mixed in a 5 mL DOX solution (phosphate-buffered saline [PBS], 0.5 mg/mL), followed by ultrasonic agitation in a type 60 Sonic Dismembrator (Fisher Scientific, Waltham, MA, USA) for 24 hours.

    Techniques: Concentration Assay, Small Interfering RNA

    Schematic representation of DOX loading and BCL2 siRNA binding with the tumor targeting co-delivery nanocarrier (FA-HP-β-CD-PEI) for forming nanocomplexes to overcome MDR and enhance apoptosis in MCF-7/Adr tumor cells. Abbreviations: DOX, doxorubicin; siRNA, small interfering RNA; mRNA, messenger RNA; MDR, multidrug resistance; FA, folic acid; HP-β-CD, hydroxypropyl-β-cyclodextrin; PEI, polyethylenimine; RISC, RNA-induced silencing complex.

    Journal: International Journal of Nanomedicine

    Article Title: Reversal of multidrug resistance in MCF-7/Adr cells by codelivery of doxorubicin and BCL2 siRNA using a folic acid-conjugated polyethylenimine hydroxypropyl-β-cyclodextrin nanocarrier

    doi: 10.2147/IJN.S67146

    Figure Lengend Snippet: Schematic representation of DOX loading and BCL2 siRNA binding with the tumor targeting co-delivery nanocarrier (FA-HP-β-CD-PEI) for forming nanocomplexes to overcome MDR and enhance apoptosis in MCF-7/Adr tumor cells. Abbreviations: DOX, doxorubicin; siRNA, small interfering RNA; mRNA, messenger RNA; MDR, multidrug resistance; FA, folic acid; HP-β-CD, hydroxypropyl-β-cyclodextrin; PEI, polyethylenimine; RISC, RNA-induced silencing complex.

    Article Snippet: Preparation and characterization of FA-HP-β-CD-PEI/DOX nanocomplexes Five milligrams of FA-HP-β-CD-PEI was mixed in a 5 mL DOX solution (phosphate-buffered saline [PBS], 0.5 mg/mL), followed by ultrasonic agitation in a type 60 Sonic Dismembrator (Fisher Scientific, Waltham, MA, USA) for 24 hours.

    Techniques: Binding Assay, Small Interfering RNA

    TEM images and DLS measure of drug-loaded nanocomplexes. Notes: ( A ) TEM imaging of FA-HP-β-CD-PEI/DOX nanocomplexes; ( B ) TEM imaging of FA-HP-β-CD-PEI/DOX/siRNA nanocomplexes; ( C ) DLS of FA-HP-β-CD-PEI/DOX nanocomplexes; ( D ) DLS of FA-HP-β-CD-PEI/DOX/siRNA nanocomplexes. The N/P ratio was 16:1, and the concentration of siRNA was 100 nM. The scale bar 500 nm ( A , B ). Abbreviations: TEM, transmission electron microscopy; DLS, dynamic light scattering; DOX, doxorubicin; siRNA, small interfering RNA; FA, folic acid; HP-β-CD, hydroxypropyl-β-cyclodextrin; PEI, polyethylenimine.

    Journal: International Journal of Nanomedicine

    Article Title: Reversal of multidrug resistance in MCF-7/Adr cells by codelivery of doxorubicin and BCL2 siRNA using a folic acid-conjugated polyethylenimine hydroxypropyl-β-cyclodextrin nanocarrier

    doi: 10.2147/IJN.S67146

    Figure Lengend Snippet: TEM images and DLS measure of drug-loaded nanocomplexes. Notes: ( A ) TEM imaging of FA-HP-β-CD-PEI/DOX nanocomplexes; ( B ) TEM imaging of FA-HP-β-CD-PEI/DOX/siRNA nanocomplexes; ( C ) DLS of FA-HP-β-CD-PEI/DOX nanocomplexes; ( D ) DLS of FA-HP-β-CD-PEI/DOX/siRNA nanocomplexes. The N/P ratio was 16:1, and the concentration of siRNA was 100 nM. The scale bar 500 nm ( A , B ). Abbreviations: TEM, transmission electron microscopy; DLS, dynamic light scattering; DOX, doxorubicin; siRNA, small interfering RNA; FA, folic acid; HP-β-CD, hydroxypropyl-β-cyclodextrin; PEI, polyethylenimine.

    Article Snippet: Preparation and characterization of FA-HP-β-CD-PEI/DOX nanocomplexes Five milligrams of FA-HP-β-CD-PEI was mixed in a 5 mL DOX solution (phosphate-buffered saline [PBS], 0.5 mg/mL), followed by ultrasonic agitation in a type 60 Sonic Dismembrator (Fisher Scientific, Waltham, MA, USA) for 24 hours.

    Techniques: Transmission Electron Microscopy, Imaging, Concentration Assay, Transmission Assay, Electron Microscopy, Small Interfering RNA

    Cell uptake of FA-HP-β-CD-PEI/DOX/siRNA FAM nanocomplexes was evaluated by CSLM ( A ) and FCM ( B ) in MCF-7/Adr cells. Notes: The free DOX, HP-β-CD-PEI/DOX/siRNA FAM , and free FA + FA-HP-β-CD-PEI/DOX/siRNA FAM were used as contrast. The tumor-targeting nanocomplexes showed the strongest fluorescence in the experiments. Incubation time, 4 hours; siRNA, 100 nM; DOX, 0.5 μg/mL; blue, Hoechst 33342, Ex 405 nm, Em 435 nm; green, siRNA FAM , Ex 488 nm, Em 535 nm; red, DOX, Ex 488 nm, Em 595 nm. Scale bars 40 μm ( A ). Abbreviations: FA, folic acid; HP-β-CD, hydroxypropyl-β-cyclodextrin; PEI, polyethylenimine; DOX, doxorubicin; siRNA FAM , small interfering RNA link with FAM green fluorescent dye; CSLM, confocal scanning laser microscopy; FCM, flow cytometry; Ex, excitation; Em, emission.

    Journal: International Journal of Nanomedicine

    Article Title: Reversal of multidrug resistance in MCF-7/Adr cells by codelivery of doxorubicin and BCL2 siRNA using a folic acid-conjugated polyethylenimine hydroxypropyl-β-cyclodextrin nanocarrier

    doi: 10.2147/IJN.S67146

    Figure Lengend Snippet: Cell uptake of FA-HP-β-CD-PEI/DOX/siRNA FAM nanocomplexes was evaluated by CSLM ( A ) and FCM ( B ) in MCF-7/Adr cells. Notes: The free DOX, HP-β-CD-PEI/DOX/siRNA FAM , and free FA + FA-HP-β-CD-PEI/DOX/siRNA FAM were used as contrast. The tumor-targeting nanocomplexes showed the strongest fluorescence in the experiments. Incubation time, 4 hours; siRNA, 100 nM; DOX, 0.5 μg/mL; blue, Hoechst 33342, Ex 405 nm, Em 435 nm; green, siRNA FAM , Ex 488 nm, Em 535 nm; red, DOX, Ex 488 nm, Em 595 nm. Scale bars 40 μm ( A ). Abbreviations: FA, folic acid; HP-β-CD, hydroxypropyl-β-cyclodextrin; PEI, polyethylenimine; DOX, doxorubicin; siRNA FAM , small interfering RNA link with FAM green fluorescent dye; CSLM, confocal scanning laser microscopy; FCM, flow cytometry; Ex, excitation; Em, emission.

    Article Snippet: Preparation and characterization of FA-HP-β-CD-PEI/DOX nanocomplexes Five milligrams of FA-HP-β-CD-PEI was mixed in a 5 mL DOX solution (phosphate-buffered saline [PBS], 0.5 mg/mL), followed by ultrasonic agitation in a type 60 Sonic Dismembrator (Fisher Scientific, Waltham, MA, USA) for 24 hours.

    Techniques: Fluorescence, Incubation, Small Interfering RNA, Microscopy, Flow Cytometry, Cytometry

    Cell apoptosis of different transportation modalities of a fixed concentration of 0.5 μg/mL of DOX in MCF-7/Adr cells after 48 hours’ treatment with differently treated groups. Notes: The apoptosis of cells was detected by flow cytometry after staining with annexin V-FITC and a PI cell-apoptosis kit; the untreated cell group was used as a control. Cells negative for both annexin V-FITC and PI staining were classified as alive, while cells that stained positive for annexin V-FITC and negative for PI were classified as apoptotic. Cells that stained positive for PI were classified as necrotic. The concentration of BCL2 siRNA used was 100 nM. Abbreviations: DOX, doxorubicin; FITC, fluorescein isothiocyanate; PI, propidium iodide; FA, folic acid; HP-β-CD, hydroxypropyl-β-cyclodextrin; PEI, polyethylenimine; siRNA, small interfering RNA.

    Journal: International Journal of Nanomedicine

    Article Title: Reversal of multidrug resistance in MCF-7/Adr cells by codelivery of doxorubicin and BCL2 siRNA using a folic acid-conjugated polyethylenimine hydroxypropyl-β-cyclodextrin nanocarrier

    doi: 10.2147/IJN.S67146

    Figure Lengend Snippet: Cell apoptosis of different transportation modalities of a fixed concentration of 0.5 μg/mL of DOX in MCF-7/Adr cells after 48 hours’ treatment with differently treated groups. Notes: The apoptosis of cells was detected by flow cytometry after staining with annexin V-FITC and a PI cell-apoptosis kit; the untreated cell group was used as a control. Cells negative for both annexin V-FITC and PI staining were classified as alive, while cells that stained positive for annexin V-FITC and negative for PI were classified as apoptotic. Cells that stained positive for PI were classified as necrotic. The concentration of BCL2 siRNA used was 100 nM. Abbreviations: DOX, doxorubicin; FITC, fluorescein isothiocyanate; PI, propidium iodide; FA, folic acid; HP-β-CD, hydroxypropyl-β-cyclodextrin; PEI, polyethylenimine; siRNA, small interfering RNA.

    Article Snippet: Preparation and characterization of FA-HP-β-CD-PEI/DOX nanocomplexes Five milligrams of FA-HP-β-CD-PEI was mixed in a 5 mL DOX solution (phosphate-buffered saline [PBS], 0.5 mg/mL), followed by ultrasonic agitation in a type 60 Sonic Dismembrator (Fisher Scientific, Waltham, MA, USA) for 24 hours.

    Techniques: Concentration Assay, Flow Cytometry, Cytometry, Staining, Small Interfering RNA

    Western blot analysis showed the efficacy in suppressing BCL2 protein expression in MCF-7/Adr cells with different treatment groups (1, control; 2, naked siRNA; 3, HiPer-Fect/siRNA; 4, siPort NeoFX/siRNA; 5, HP-β-CD-PEI/siRNA; 6, HP-β-CD-PEI/DOX/siRNA; 7, FA-HP-β-CD-PEI/siRNA; 8, FA-HP-β-CD-PEI/DOX/siRNA). Notes: The purchased siRNA reagents (HiPerFect from Qiagen, siPort NeoFX from Invitrogen) were used as the positive control. A significant difference was observed between control and different treatment groups. Data shown here are the mean ± standard deviation of three independent experiments. Incubation, 72 hours; siRNA concentration, 100 nM. * P

    Journal: International Journal of Nanomedicine

    Article Title: Reversal of multidrug resistance in MCF-7/Adr cells by codelivery of doxorubicin and BCL2 siRNA using a folic acid-conjugated polyethylenimine hydroxypropyl-β-cyclodextrin nanocarrier

    doi: 10.2147/IJN.S67146

    Figure Lengend Snippet: Western blot analysis showed the efficacy in suppressing BCL2 protein expression in MCF-7/Adr cells with different treatment groups (1, control; 2, naked siRNA; 3, HiPer-Fect/siRNA; 4, siPort NeoFX/siRNA; 5, HP-β-CD-PEI/siRNA; 6, HP-β-CD-PEI/DOX/siRNA; 7, FA-HP-β-CD-PEI/siRNA; 8, FA-HP-β-CD-PEI/DOX/siRNA). Notes: The purchased siRNA reagents (HiPerFect from Qiagen, siPort NeoFX from Invitrogen) were used as the positive control. A significant difference was observed between control and different treatment groups. Data shown here are the mean ± standard deviation of three independent experiments. Incubation, 72 hours; siRNA concentration, 100 nM. * P

    Article Snippet: Preparation and characterization of FA-HP-β-CD-PEI/DOX nanocomplexes Five milligrams of FA-HP-β-CD-PEI was mixed in a 5 mL DOX solution (phosphate-buffered saline [PBS], 0.5 mg/mL), followed by ultrasonic agitation in a type 60 Sonic Dismembrator (Fisher Scientific, Waltham, MA, USA) for 24 hours.

    Techniques: Western Blot, Expressing, Positive Control, Standard Deviation, Incubation, Concentration Assay

    Morphological study of MCF-7/Adr cells after treatment with different modalities for 48 hours by Bio-TEM. Notes: TEM of MCF-7/Adr cells after treatment with different modalities showed the major ultrastructural changes for each sample. Concentrations of DOX and BCL2 siRNA were 0.5 μg/mL and 100 nM, respectively. Abbreviations: TEM, transmission electron microscopy; DOX, doxorubicin; siRNA, small interfering RNA; FA, folic acid; HP-β-CD, hydroxypropyl-β-cyclodextrin; PEI, polyethylenimine.

    Journal: International Journal of Nanomedicine

    Article Title: Reversal of multidrug resistance in MCF-7/Adr cells by codelivery of doxorubicin and BCL2 siRNA using a folic acid-conjugated polyethylenimine hydroxypropyl-β-cyclodextrin nanocarrier

    doi: 10.2147/IJN.S67146

    Figure Lengend Snippet: Morphological study of MCF-7/Adr cells after treatment with different modalities for 48 hours by Bio-TEM. Notes: TEM of MCF-7/Adr cells after treatment with different modalities showed the major ultrastructural changes for each sample. Concentrations of DOX and BCL2 siRNA were 0.5 μg/mL and 100 nM, respectively. Abbreviations: TEM, transmission electron microscopy; DOX, doxorubicin; siRNA, small interfering RNA; FA, folic acid; HP-β-CD, hydroxypropyl-β-cyclodextrin; PEI, polyethylenimine.

    Article Snippet: Preparation and characterization of FA-HP-β-CD-PEI/DOX nanocomplexes Five milligrams of FA-HP-β-CD-PEI was mixed in a 5 mL DOX solution (phosphate-buffered saline [PBS], 0.5 mg/mL), followed by ultrasonic agitation in a type 60 Sonic Dismembrator (Fisher Scientific, Waltham, MA, USA) for 24 hours.

    Techniques: Transmission Electron Microscopy, Transmission Assay, Electron Microscopy, Small Interfering RNA