phosphate buffered saline pbs solution  (Cell Signaling Technology Inc)

 
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    Name:
    Phosphate Buffered Saline with Tween 20 PBST 20X
    Description:
    Phosphate Buffered Saline PBS solution with the detergent Tween 20 for use as a wash buffer and diluent for ELISA 1 X PBST contains 3 2 mM Na2HPO4 0 5 mM KH2PO4 1 3 mM KCl 135 mM NaCl 0 05 Tween 20 pH 7 4
    Catalog Number:
    9809
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    Buffers Dyes
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    Structured Review

    Cell Signaling Technology Inc phosphate buffered saline pbs solution
    Phosphate Buffered Saline PBS solution with the detergent Tween 20 for use as a wash buffer and diluent for ELISA 1 X PBST contains 3 2 mM Na2HPO4 0 5 mM KH2PO4 1 3 mM KCl 135 mM NaCl 0 05 Tween 20 pH 7 4
    https://www.bioz.com/result/phosphate buffered saline pbs solution/product/Cell Signaling Technology Inc
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    phosphate buffered saline pbs solution - by Bioz Stars, 2020-08
    99/100 stars

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    Incubation:

    Article Title: Hyperactive Ras/MAPK signaling is critical for tibial nonunion fracture in neurofibromin-deficient mice
    Article Snippet: .. After blocking in 5% nonfat dry milk in phosphate buffered saline tween-20 (PBST), the membranes were incubated with primary antibodies at 1:1000 dilution (phospho-ERK, total-ERK, β-Actin; Cell Signaling) in 5% milk in PBST overnight at 4°C. .. Following overnight exposure, the membranes were washed three times with PBST and incubated with secondary antibodies (anti-rabbit, anti-mouse, GE Healthcare UK Limited, UK) conjugated with horseradish peroxidase at 1:5000 dilution in 5% milk in PBST for 1 h at room temperature.

    Article Title: Chloroquine sensitizes MDA-MB-231 cells to osimertinib through autophagy–apoptosis crosstalk pathway
    Article Snippet: .. The PVDF membrane was blocked using 5% nonfat dry milk in 1⨰ PBST (9809S; Cell Signaling) for 60 mins at room temperature, incubated with primary antibodies overnight with gentle agitation at 4°C, washed with PBST, and incubated with secondary antibody for 1 hr at room temperature. .. Blots were imaged using chemiluminescence (ECL; Bio-Rad), and densitometric analysis was carried out with ImageLab to quantitate the levels of LC3B-II normalized with GAPDH.

    Article Title: Radiation mitigation of the intestinal acute radiation injury in mice by 1‐[(4‐nitrophenyl)sulfonyl]‐4‐phenylpiperazine, et al. Radiation mitigation of the intestinal acute radiation injury in mice by 1‐[(4‐nitrophenyl)sulfonyl]‐4‐phenylpiperazine
    Article Snippet: .. The slides rinsed in phosphate buffered saline containing 0.05% tween‐20 (PBST), and then incubated with a rabbit anti‐mouse Ki67 antibody (Cell Signaling, Danvers, MA, #12202; 1:200) or rabbit anti‐β‐catenin antibody (Cell Signaling, #9562; 1:50) at RT for 1 hour and with a rabbit anti‐Notch1‐ICD antibody (Abcam, #ab8925, 1:50, Cambridge, UK) overnight at 4°C. .. The slides were rinsed with PBST, and were incubated with secondary polymer at RT for 30 minutes; the polymers used were Dako EnVision + System‐HRP Labeled Polymer anti‐rabbit (Dako, Carpinteria, CA, #K4003) for Ki67 or MACH3 rabbit HRP polymer (Biocare Medical, Concord, California) for β‐catenin.

    Article Title: Antimicrobial activity and acetylcholinestrase inhibition of novel synthesized pyrimidine derivatives versus Candida albicans trafficking to brain and kidney
    Article Snippet: .. These proteins were separated then relocated into membranes of nitrocellulose. the membranes then blocked with 5% dry milk in phosphate buffered saline with 0.1% Tween20 then incubated overnight at 4 °C with primary COX-2 antibody (Cell Signaling Technology, Beverly, MA, USA) [ ]. .. 2.10 Statistical analysis Row data were analyzed and termed as means ± S E M. Statistics was completed by GraghPad Istat-3 program (GraphPad Inc., San Diego, CA).

    Article Title: Identification of Antibody Targets for Tuberculosis Serology using High-Density Nucleic Acid Programmable Protein Arrays *
    Article Snippet: .. After sealing the wells with a polystyrene membrane under 200 PSI pressure, we incubated the reactor for 2 h at 30 °C for expression and for 0.5 h at 15 °C for protein capture, followed by blocking with 5% skim milk in phosphate buffered saline with 0.2% tween 20 (PBST) for 30 min. Anti-GST murine monoclonal Ab (mAb; Cell signaling technology, Danvers, MA) was used to assess protein display followed by detection with Alexa 647-labeled Goat anti-mouse IgG (H+L) secondary Ab (A-21235, Thermo Fisher Scientific) ( ). ..

    Generated:

    Article Title: Pre-clinical pharmacology of AZD3965, a selective inhibitor of MCT1: DLBCL, NHL and Burkitt’s lymphoma anti-tumor activity
    Article Snippet: .. Membranes were blocked with 5% (w/v) non-fat milk in phosphate buffered saline + Tween 20 (3.2 mM Na2 HPO4 , 0.5 mM KH2 PO4 , 1.3 mM KCl, 135 mM NaCl, 0.05% Tween 20, pH 7.4) and probed overnight with antibodies for PARP (Cell Signaling Technology, Inc.), cleaved PARP (BD Biosciences), MCT1 (in-house generated polyclonal), MCT4 (in-house generated polyclonal) and β-actin loading control (Sigma). .. Membranes were then washed and incubated with horseradish peroxidase-tagged secondary antibodies, followed by detection on a Syngene ChemiGenius with Super Signal West Dura Chemiluminescence Substrate.

    Blocking Assay:

    Article Title: Hyperactive Ras/MAPK signaling is critical for tibial nonunion fracture in neurofibromin-deficient mice
    Article Snippet: .. After blocking in 5% nonfat dry milk in phosphate buffered saline tween-20 (PBST), the membranes were incubated with primary antibodies at 1:1000 dilution (phospho-ERK, total-ERK, β-Actin; Cell Signaling) in 5% milk in PBST overnight at 4°C. .. Following overnight exposure, the membranes were washed three times with PBST and incubated with secondary antibodies (anti-rabbit, anti-mouse, GE Healthcare UK Limited, UK) conjugated with horseradish peroxidase at 1:5000 dilution in 5% milk in PBST for 1 h at room temperature.

    Article Title: Complex regulation of the regulator of synaptic plasticity histone deacetylase 2 in the rodent dorsal horn after peripheral injury
    Article Snippet: .. After blocking for 1 h at 20–21 °C in 4% milk/0.1% phosphate‐buffered saline‐Tween20, the membranes were incubate with primary antibody (anti‐nNOS 1 : 2000, Cell Signalling, Danvers, MA, USA; Anti‐HDAC2 1 : 2000, Abcam, Cambridge, UK) O/N at 4°C. ..

    Article Title: Identification of Antibody Targets for Tuberculosis Serology using High-Density Nucleic Acid Programmable Protein Arrays *
    Article Snippet: .. After sealing the wells with a polystyrene membrane under 200 PSI pressure, we incubated the reactor for 2 h at 30 °C for expression and for 0.5 h at 15 °C for protein capture, followed by blocking with 5% skim milk in phosphate buffered saline with 0.2% tween 20 (PBST) for 30 min. Anti-GST murine monoclonal Ab (mAb; Cell signaling technology, Danvers, MA) was used to assess protein display followed by detection with Alexa 647-labeled Goat anti-mouse IgG (H+L) secondary Ab (A-21235, Thermo Fisher Scientific) ( ). ..

    Expressing:

    Article Title: Identification of Antibody Targets for Tuberculosis Serology using High-Density Nucleic Acid Programmable Protein Arrays *
    Article Snippet: .. After sealing the wells with a polystyrene membrane under 200 PSI pressure, we incubated the reactor for 2 h at 30 °C for expression and for 0.5 h at 15 °C for protein capture, followed by blocking with 5% skim milk in phosphate buffered saline with 0.2% tween 20 (PBST) for 30 min. Anti-GST murine monoclonal Ab (mAb; Cell signaling technology, Danvers, MA) was used to assess protein display followed by detection with Alexa 647-labeled Goat anti-mouse IgG (H+L) secondary Ab (A-21235, Thermo Fisher Scientific) ( ). ..

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    Cell Signaling Technology Inc 4 6 diamidino 2 phenylindole dapi solution solution cell signaling technology danvers ma usa
    Characterization of human primary prostate epithelial cell cultures. Notes: ( A ) Primary outgrowth of epithelial cells derived from a biopsy specimen. ( B ) Primary epithelial cell colony. ( C ) Characterization of epithelial cells: green fluorescence shows high-molecular-weight cytokeratins; blue fluorescence shows nuclei reactive with <t>4′,6-diamidino-2-phenylindole</t> <t>(DAPI).</t> No vimentin staining was observed in primary epithelial cell cultures (data not shown).
    4 6 Diamidino 2 Phenylindole Dapi Solution Solution Cell Signaling Technology Danvers Ma Usa, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    4 6 diamidino 2 phenylindole dapi solution solution cell signaling technology danvers ma usa - by Bioz Stars, 2020-08
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    Cell Signaling Technology Inc pbs blocking solution
    Characterization of human primary prostate epithelial cell cultures. Notes: ( A ) Primary outgrowth of epithelial cells derived from a biopsy specimen. ( B ) Primary epithelial cell colony. ( C ) Characterization of epithelial cells: green fluorescence shows high-molecular-weight cytokeratins; blue fluorescence shows nuclei reactive with <t>4′,6-diamidino-2-phenylindole</t> <t>(DAPI).</t> No vimentin staining was observed in primary epithelial cell cultures (data not shown).
    Pbs Blocking Solution, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc pbs t solution
    Characterization of human primary prostate epithelial cell cultures. Notes: ( A ) Primary outgrowth of epithelial cells derived from a biopsy specimen. ( B ) Primary epithelial cell colony. ( C ) Characterization of epithelial cells: green fluorescence shows high-molecular-weight cytokeratins; blue fluorescence shows nuclei reactive with <t>4′,6-diamidino-2-phenylindole</t> <t>(DAPI).</t> No vimentin staining was observed in primary epithelial cell cultures (data not shown).
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    Cell Signaling Technology Inc triton x 100 solution
    Characterization of human primary prostate epithelial cell cultures. Notes: ( A ) Primary outgrowth of epithelial cells derived from a biopsy specimen. ( B ) Primary epithelial cell colony. ( C ) Characterization of epithelial cells: green fluorescence shows high-molecular-weight cytokeratins; blue fluorescence shows nuclei reactive with <t>4′,6-diamidino-2-phenylindole</t> <t>(DAPI).</t> No vimentin staining was observed in primary epithelial cell cultures (data not shown).
    Triton X 100 Solution, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Characterization of human primary prostate epithelial cell cultures. Notes: ( A ) Primary outgrowth of epithelial cells derived from a biopsy specimen. ( B ) Primary epithelial cell colony. ( C ) Characterization of epithelial cells: green fluorescence shows high-molecular-weight cytokeratins; blue fluorescence shows nuclei reactive with 4′,6-diamidino-2-phenylindole (DAPI). No vimentin staining was observed in primary epithelial cell cultures (data not shown).

    Journal: Research and Reports in Urology

    Article Title: IL-8 secretion in primary cultures of prostate cells is associated with prostate cancer aggressiveness

    doi: 10.2147/RRU.S58643

    Figure Lengend Snippet: Characterization of human primary prostate epithelial cell cultures. Notes: ( A ) Primary outgrowth of epithelial cells derived from a biopsy specimen. ( B ) Primary epithelial cell colony. ( C ) Characterization of epithelial cells: green fluorescence shows high-molecular-weight cytokeratins; blue fluorescence shows nuclei reactive with 4′,6-diamidino-2-phenylindole (DAPI). No vimentin staining was observed in primary epithelial cell cultures (data not shown).

    Article Snippet: Slides were washed in PBS/0.025% Tween 20 three times for 5 minutes, after which the nuclei were stained by incubating the slides for 10 minutes in a 1 μg/mL 4′,6-diamidino-2-phenylindole (DAPI) solution (Cell Signaling Technology, Danvers, MA, USA [1 μg/mL in PBS]).

    Techniques: Derivative Assay, Fluorescence, Molecular Weight, Staining