phosphate buffered saline pbs solution  (Boster Bio)

 
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    Name:
    Phosphate Buffered Saline PBS Powder
    Description:
    Boster†s Phosphate Buffered Saline PBS Powder is a dehydrated raw material reagent intended to be used in the making of prepared media products by reconstitution in distilled water
    Catalog Number:
    AR0030
    Price:
    3.0
    Conjugate:
    No
    Size:
    1 pack 2000 ml
    Category:
    Buffers In Dry Powder form
    		Buy from Supplier

    Structured Review

    Boster Bio phosphate buffered saline pbs solution
    Boster†s Phosphate Buffered Saline PBS Powder is a dehydrated raw material reagent intended to be used in the making of prepared media products by reconstitution in distilled water
    https://www.bioz.com/result/phosphate buffered saline pbs solution/product/Boster Bio
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphate buffered saline pbs solution - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    ROS Assay:

    Article Title: Angelica sinensis polysaccharide prevents mitochondrial apoptosis by regulating the Treg/Th17 ratio in aplastic anemia
    Article Snippet: .. A functional mitochondria isolation kit, mitochondrial outer membrane integrity testing kit, mitochondrial inner membrane integrity testing kit, purified mitochondrial cytochrome C oxidase activity assay kit, reactive oxygen species (ROS) assay kit and phosphate-buffered saline (PBS) were obtained from Wuhan Boster Biotechnology, Ltd. (Wuhan, China). .. The cell lysis buffer and horseradish peroxidase-conjugated secondary antibodies used for Western blotting were obtained from Beyotime Biotechnology (Shanghai, China).

    Functional Assay:

    Article Title: Angelica sinensis polysaccharide prevents mitochondrial apoptosis by regulating the Treg/Th17 ratio in aplastic anemia
    Article Snippet: .. A functional mitochondria isolation kit, mitochondrial outer membrane integrity testing kit, mitochondrial inner membrane integrity testing kit, purified mitochondrial cytochrome C oxidase activity assay kit, reactive oxygen species (ROS) assay kit and phosphate-buffered saline (PBS) were obtained from Wuhan Boster Biotechnology, Ltd. (Wuhan, China). .. The cell lysis buffer and horseradish peroxidase-conjugated secondary antibodies used for Western blotting were obtained from Beyotime Biotechnology (Shanghai, China).

    Binding Assay:

    Article Title: Expression of TLR2/4 in the sperm‐storing oviduct of the Chinese soft‐shelled turtle Pelodiscus sinensis during hibernation season
    Article Snippet: .. After three washes in the phosphate‐buffered saline (PBS), the slides were incubated in citrate buffer solution in boiling water for 15 min. After washing three times with PBS, the slides were incubated in the 5% BSA solution from the sABC Immunohistochemistry Kit (Boster, China) for 30 min in a humidified chamber to block nonspecific binding. .. The sections were incubated at 4°C for 15 h with 200 μ g/mL of a rabbit polyclonal antibody to human, mouse, and rat TLR4 (Boster, China).

    Isolation:

    Article Title: Angelica sinensis polysaccharide prevents mitochondrial apoptosis by regulating the Treg/Th17 ratio in aplastic anemia
    Article Snippet: .. A functional mitochondria isolation kit, mitochondrial outer membrane integrity testing kit, mitochondrial inner membrane integrity testing kit, purified mitochondrial cytochrome C oxidase activity assay kit, reactive oxygen species (ROS) assay kit and phosphate-buffered saline (PBS) were obtained from Wuhan Boster Biotechnology, Ltd. (Wuhan, China). .. The cell lysis buffer and horseradish peroxidase-conjugated secondary antibodies used for Western blotting were obtained from Beyotime Biotechnology (Shanghai, China).

    Immunohistochemistry:

    Article Title: Expression of TLR2/4 in the sperm‐storing oviduct of the Chinese soft‐shelled turtle Pelodiscus sinensis during hibernation season
    Article Snippet: .. After three washes in the phosphate‐buffered saline (PBS), the slides were incubated in citrate buffer solution in boiling water for 15 min. After washing three times with PBS, the slides were incubated in the 5% BSA solution from the sABC Immunohistochemistry Kit (Boster, China) for 30 min in a humidified chamber to block nonspecific binding. .. The sections were incubated at 4°C for 15 h with 200 μ g/mL of a rabbit polyclonal antibody to human, mouse, and rat TLR4 (Boster, China).

    Blocking Assay:

    Article Title: Expression of TLR2/4 in the sperm‐storing oviduct of the Chinese soft‐shelled turtle Pelodiscus sinensis during hibernation season
    Article Snippet: .. After three washes in the phosphate‐buffered saline (PBS), the slides were incubated in citrate buffer solution in boiling water for 15 min. After washing three times with PBS, the slides were incubated in the 5% BSA solution from the sABC Immunohistochemistry Kit (Boster, China) for 30 min in a humidified chamber to block nonspecific binding. .. The sections were incubated at 4°C for 15 h with 200 μ g/mL of a rabbit polyclonal antibody to human, mouse, and rat TLR4 (Boster, China).

    Avidin-Biotin Assay:

    Article Title: Alteration of Podocyte Protein Expression and Localization in the Early Stage of Various Hemodynamic Conditions
    Article Snippet: .. After washing in phosphate buffered saline (PBS), the sections were repaired with sodium citrate buffer liquid at high pressure and incubated with PBS containing 5% normal bovine serum (Boster, Wuhan, China) for 1 h at 37 °C, followed by incubation in the primary antibodies at 4 °C overnight and corresponding secondary antibodies at 37 °C for 1 h. Then, the sections were incubated with a horseradish peroxidase (HRP)-conjugated avidin-biotin complex (ABC) for 20 min, and the staining was visualized with metal-enhanced 3,3′-diaminobenzidine (DAB) (Boster, Wuhan, China) for 5 min (ABC-DAB method). .. Finally, they were sealed with peucine and photographed under a light microscope or a confocal laser scanning microscope (FV10-ASW2.1Viewer).

    Incubation:

    Article Title: Expression of TLR2/4 in the sperm‐storing oviduct of the Chinese soft‐shelled turtle Pelodiscus sinensis during hibernation season
    Article Snippet: .. After three washes in the phosphate‐buffered saline (PBS), the slides were incubated in citrate buffer solution in boiling water for 15 min. After washing three times with PBS, the slides were incubated in the 5% BSA solution from the sABC Immunohistochemistry Kit (Boster, China) for 30 min in a humidified chamber to block nonspecific binding. .. The sections were incubated at 4°C for 15 h with 200 μ g/mL of a rabbit polyclonal antibody to human, mouse, and rat TLR4 (Boster, China).

    Article Title: Alteration of Podocyte Protein Expression and Localization in the Early Stage of Various Hemodynamic Conditions
    Article Snippet: .. After washing in phosphate buffered saline (PBS), the sections were repaired with sodium citrate buffer liquid at high pressure and incubated with PBS containing 5% normal bovine serum (Boster, Wuhan, China) for 1 h at 37 °C, followed by incubation in the primary antibodies at 4 °C overnight and corresponding secondary antibodies at 37 °C for 1 h. Then, the sections were incubated with a horseradish peroxidase (HRP)-conjugated avidin-biotin complex (ABC) for 20 min, and the staining was visualized with metal-enhanced 3,3′-diaminobenzidine (DAB) (Boster, Wuhan, China) for 5 min (ABC-DAB method). .. Finally, they were sealed with peucine and photographed under a light microscope or a confocal laser scanning microscope (FV10-ASW2.1Viewer).

    Article Title: Isolation and Characterization of Two Kinds of Stem Cells from the Same Human Skin Back Sample with Therapeutic Potential in Spinal Cord Injury
    Article Snippet: .. Skin tissues were washed with cold phosphate-buffered saline (PBS, Boster, Wu Han, China, China), cut into 4–6-mm2 pieces and incubated in trypsin (Invitrogen, Carlsbad, CA) overnight at 4°C. .. Epidermis was manually removed and the dermis was cut into 1-mm3 pieces and incubated in trypsin for 30 minutes at 37°C.

    Article Title: Sema7A, a brain immune regulator, regulates seizure activity in PTZ‐kindled epileptic rats, et al. Sema7A, a brain immune regulator, regulates seizure activity in PTZ‐kindled epileptic rats
    Article Snippet: .. After being washed with phosphate‐buffered saline (PBS) three times, the sections were blocked in 5% goat serum (Boster Biological Technology) for 1 hour at room temperature and then incubated with a mixture of a rabbit polyclonal anti‐Sema7A antibody (1:50, GeneTex) and an anti‐neuron‐specific enolase (NSE) mouse monoclonal antibody (1:50, Proteintech) or with a mixture of a rabbit polyclonal anti‐Sema7A antibody (1:50) and mouse monoclonal anti‐glial fibrillary acidic protein (GFAP) antibody (1:100, Boster Biological Technology) at 4°C overnight. .. The sections were washed with PBS and incubated with a mixture of fluorescein isothiocyanate (FITC)‐conjugated goat anti‐rabbit IgG (1:100, Beyotime) and Cy3‐labeled goat anti‐mouse IgG (1:200, Beyotime, Shanghai, China) in the dark for 60 minutes at 37°C.

    other:

    Article Title: A Dual-Modality MR/PA Imaging Contrast Agent Based on Ultrasmall Biopolymer Nanoparticles for Orthotopic Hepatocellular Carcinoma Imaging
    Article Snippet: Gadolinium(III)chloride hexahydrate (GdCl3 ·6H2 O), sodium hydroxide (NaOH) and hydrochloric acid (37 wt% HCl) were acquired from Sinopharm Chemical Reagent Beijing Co., Ltd. Amine-PEG5000 -amine (NH2 -PEG5000 -NH2 , 5 kDa) was obtained from Shanghai Zzbio Co., Ltd. Phosphate-buffered saline (PBS) and 4,6-diamidino-2-phenylindole (DAPI) was purchased from Boster biological Technology Co., Ltd. Fluorescein isothiocyanate were acquired from ACROS (90%, FITC).

    Activity Assay:

    Article Title: Angelica sinensis polysaccharide prevents mitochondrial apoptosis by regulating the Treg/Th17 ratio in aplastic anemia
    Article Snippet: .. A functional mitochondria isolation kit, mitochondrial outer membrane integrity testing kit, mitochondrial inner membrane integrity testing kit, purified mitochondrial cytochrome C oxidase activity assay kit, reactive oxygen species (ROS) assay kit and phosphate-buffered saline (PBS) were obtained from Wuhan Boster Biotechnology, Ltd. (Wuhan, China). .. The cell lysis buffer and horseradish peroxidase-conjugated secondary antibodies used for Western blotting were obtained from Beyotime Biotechnology (Shanghai, China).

    Microscopy:

    Article Title: Association between FOXM1 and hedgehog signaling pathway in human cervical carcinoma by tissue microarray analysis
    Article Snippet: .. Next, the slides were counterstained with hematoxylin (catalogue no. H9627, Sigma-Aldrich, St. Louis, MO, USA), rinsed several times with phosphate-buffered saline (PBS; Wuhan Boster Biological Technology, Ltd.) pH 7.4, dehydrated with a series of graded ethanol solutions, cleared using xylene (Sinopharm Group Co., Ltd.), and observed under an ECLIPSE E100 optical microscope (Nikon Corporation, Tokyo, Japan). .. To detect SHh and PTCH1 antigens, the endogenous peroxidase activity was blocked with rabbit serum (Shanghai Beiyi Bioequip Information Co., Ltd., Shanghai, China) for 30 min at room temperature, prior to incubating the tissue sections with the corresponding primary antibodies overnight at 4°C.

    Staining:

    Article Title: Alteration of Podocyte Protein Expression and Localization in the Early Stage of Various Hemodynamic Conditions
    Article Snippet: .. After washing in phosphate buffered saline (PBS), the sections were repaired with sodium citrate buffer liquid at high pressure and incubated with PBS containing 5% normal bovine serum (Boster, Wuhan, China) for 1 h at 37 °C, followed by incubation in the primary antibodies at 4 °C overnight and corresponding secondary antibodies at 37 °C for 1 h. Then, the sections were incubated with a horseradish peroxidase (HRP)-conjugated avidin-biotin complex (ABC) for 20 min, and the staining was visualized with metal-enhanced 3,3′-diaminobenzidine (DAB) (Boster, Wuhan, China) for 5 min (ABC-DAB method). .. Finally, they were sealed with peucine and photographed under a light microscope or a confocal laser scanning microscope (FV10-ASW2.1Viewer).

    Purification:

    Article Title: Angelica sinensis polysaccharide prevents mitochondrial apoptosis by regulating the Treg/Th17 ratio in aplastic anemia
    Article Snippet: .. A functional mitochondria isolation kit, mitochondrial outer membrane integrity testing kit, mitochondrial inner membrane integrity testing kit, purified mitochondrial cytochrome C oxidase activity assay kit, reactive oxygen species (ROS) assay kit and phosphate-buffered saline (PBS) were obtained from Wuhan Boster Biotechnology, Ltd. (Wuhan, China). .. The cell lysis buffer and horseradish peroxidase-conjugated secondary antibodies used for Western blotting were obtained from Beyotime Biotechnology (Shanghai, China).

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    Boster Bio mouse anti vimentin antibody solution
    Expression of cell-specific marker in somatic cells derived from ear skin of buffalo. a Immunofluorescent staining of <t>vimentin</t> for fibroblasts, b negative control for vimentin (50 µm)
    Mouse Anti Vimentin Antibody Solution, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti vimentin antibody solution/product/Boster Bio
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti vimentin antibody solution - by Bioz Stars, 2020-08
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    pbs solution  (Boster Bio)
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    Boster Bio pbs solution
    Expression of cell-specific marker in somatic cells derived from ear skin of buffalo. a Immunofluorescent staining of <t>vimentin</t> for fibroblasts, b negative control for vimentin (50 µm)
    Pbs Solution, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs solution/product/Boster Bio
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    pbs solution - by Bioz Stars, 2020-08
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    phosphate buffer solution pbs  (Boster Bio)
    91
    Boster Bio phosphate buffer solution pbs
    Expression of cell-specific marker in somatic cells derived from ear skin of buffalo. a Immunofluorescent staining of <t>vimentin</t> for fibroblasts, b negative control for vimentin (50 µm)
    Phosphate Buffer Solution Pbs, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffer solution pbs/product/Boster Bio
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    phosphate buffer solution pbs - by Bioz Stars, 2020-08
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    Expression of cell-specific marker in somatic cells derived from ear skin of buffalo. a Immunofluorescent staining of vimentin for fibroblasts, b negative control for vimentin (50 µm)

    Journal: Cytotechnology

    Article Title: A comparative study on efficiency of adult fibroblasts and amniotic fluid-derived stem cells as donor cells for production of hand-made cloned buffalo (Bubalus bubalis) embryos

    doi: 10.1007/s10616-014-9805-1

    Figure Lengend Snippet: Expression of cell-specific marker in somatic cells derived from ear skin of buffalo. a Immunofluorescent staining of vimentin for fibroblasts, b negative control for vimentin (50 µm)

    Article Snippet: Samples were then treated with an acetone–methanol (1:1) solution for 15–20 min at room temperature and incubated in a penetrant solution (PBS with 1 % BSA and 0.1 % Tween-20) for 1 h. Subsequently, these were incubated in mouse anti-vimentin antibody solution (1:200 diluted with PBS; BM0135, BOSTER, Pleasanton, CA, USA) and mouse anti-keratin antibody solution (1:200 diluted with PBS; BM0030, BOSTER) overnight at 4 °C.

    Techniques: Expressing, Marker, Derivative Assay, Staining, Negative Control