phosphate buffered saline pbs solution  (Boster Bio)


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    Name:
    Phosphate Buffered Saline PBS Powder
    Description:
    Boster†s Phosphate Buffered Saline PBS Powder is a dehydrated raw material reagent intended to be used in the making of prepared media products by reconstitution in distilled water
    Catalog Number:
    AR0030
    Price:
    3.0
    Conjugate:
    No
    Size:
    1 pack 2000 ml
    Category:
    Buffers In Dry Powder form
    		Buy from Supplier

    Structured Review

    Boster Bio phosphate buffered saline pbs solution
    Boster†s Phosphate Buffered Saline PBS Powder is a dehydrated raw material reagent intended to be used in the making of prepared media products by reconstitution in distilled water
    https://www.bioz.com/result/phosphate buffered saline pbs solution/product/Boster Bio
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphate buffered saline pbs solution - by Bioz Stars, 2021-01
    99/100 stars

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    Images

    Related Articles

    ROS Assay:

    Article Title: Angelica sinensis polysaccharide prevents mitochondrial apoptosis by regulating the Treg/Th17 ratio in aplastic anemia
    Article Snippet: .. A functional mitochondria isolation kit, mitochondrial outer membrane integrity testing kit, mitochondrial inner membrane integrity testing kit, purified mitochondrial cytochrome C oxidase activity assay kit, reactive oxygen species (ROS) assay kit and phosphate-buffered saline (PBS) were obtained from Wuhan Boster Biotechnology, Ltd. (Wuhan, China). .. The cell lysis buffer and horseradish peroxidase-conjugated secondary antibodies used for Western blotting were obtained from Beyotime Biotechnology (Shanghai, China).

    Functional Assay:

    Article Title: Angelica sinensis polysaccharide prevents mitochondrial apoptosis by regulating the Treg/Th17 ratio in aplastic anemia
    Article Snippet: .. A functional mitochondria isolation kit, mitochondrial outer membrane integrity testing kit, mitochondrial inner membrane integrity testing kit, purified mitochondrial cytochrome C oxidase activity assay kit, reactive oxygen species (ROS) assay kit and phosphate-buffered saline (PBS) were obtained from Wuhan Boster Biotechnology, Ltd. (Wuhan, China). .. The cell lysis buffer and horseradish peroxidase-conjugated secondary antibodies used for Western blotting were obtained from Beyotime Biotechnology (Shanghai, China).

    Isolation:

    Article Title: Angelica sinensis polysaccharide prevents mitochondrial apoptosis by regulating the Treg/Th17 ratio in aplastic anemia
    Article Snippet: .. A functional mitochondria isolation kit, mitochondrial outer membrane integrity testing kit, mitochondrial inner membrane integrity testing kit, purified mitochondrial cytochrome C oxidase activity assay kit, reactive oxygen species (ROS) assay kit and phosphate-buffered saline (PBS) were obtained from Wuhan Boster Biotechnology, Ltd. (Wuhan, China). .. The cell lysis buffer and horseradish peroxidase-conjugated secondary antibodies used for Western blotting were obtained from Beyotime Biotechnology (Shanghai, China).

    Mouse Assay:

    Article Title: Short hairpin RNA attenuates liver fibrosis by regulating the PPAR-γ and NF-κB pathways in HBV-induced liver fibrosis in mice
    Article Snippet: .. At the end of the experiment, mice were anesthetized with 2.5% avertin and perfused with cold PBS (pH 7.4) transcardially, followed by 4% paraformaldehyde (over 2 h at 25°C) in PBS (0.1 M, pH 7.4) (Boster Biological Technology Co., Ltd.) to fix tissues for immunohistochemistry (IHC). .. Livers were then collected for IHC analysis.

    Immunohistochemistry:

    Article Title: Short hairpin RNA attenuates liver fibrosis by regulating the PPAR-γ and NF-κB pathways in HBV-induced liver fibrosis in mice
    Article Snippet: .. At the end of the experiment, mice were anesthetized with 2.5% avertin and perfused with cold PBS (pH 7.4) transcardially, followed by 4% paraformaldehyde (over 2 h at 25°C) in PBS (0.1 M, pH 7.4) (Boster Biological Technology Co., Ltd.) to fix tissues for immunohistochemistry (IHC). .. Livers were then collected for IHC analysis.

    Microscopy:

    Article Title: Association between FOXM1 and hedgehog signaling pathway in human cervical carcinoma by tissue microarray analysis
    Article Snippet: .. Next, the slides were counterstained with hematoxylin (catalogue no. H9627, Sigma-Aldrich, St. Louis, MO, USA), rinsed several times with phosphate-buffered saline (PBS; Wuhan Boster Biological Technology, Ltd.) pH 7.4, dehydrated with a series of graded ethanol solutions, cleared using xylene (Sinopharm Group Co., Ltd.), and observed under an ECLIPSE E100 optical microscope (Nikon Corporation, Tokyo, Japan). .. To detect SHh and PTCH1 antigens, the endogenous peroxidase activity was blocked with rabbit serum (Shanghai Beiyi Bioequip Information Co., Ltd., Shanghai, China) for 30 min at room temperature, prior to incubating the tissue sections with the corresponding primary antibodies overnight at 4°C.

    Avidin-Biotin Assay:

    Article Title: Alteration of Podocyte Protein Expression and Localization in the Early Stage of Various Hemodynamic Conditions
    Article Snippet: .. After washing in phosphate buffered saline (PBS), the sections were repaired with sodium citrate buffer liquid at high pressure and incubated with PBS containing 5% normal bovine serum (Boster, Wuhan, China) for 1 h at 37 °C, followed by incubation in the primary antibodies at 4 °C overnight and corresponding secondary antibodies at 37 °C for 1 h. Then, the sections were incubated with a horseradish peroxidase (HRP)-conjugated avidin-biotin complex (ABC) for 20 min, and the staining was visualized with metal-enhanced 3,3′-diaminobenzidine (DAB) (Boster, Wuhan, China) for 5 min (ABC-DAB method). .. Finally, they were sealed with peucine and photographed under a light microscope or a confocal laser scanning microscope (FV10-ASW2.1Viewer).

    Incubation:

    Article Title: Jinmaitong Ameliorates Diabetic Peripheral Neuropathy Through Suppressing TXNIP/NLRP3 Inflammasome Activation In The Streptozotocin-Induced Diabetic Rat Model
    Article Snippet: .. After washing with phosphate-buffered saline with Tween-20 (PBST, pH 7.4), the sections were incubated in 3% H2 O2 solution and blocked with 10% normal goat serum (Boster, Wuhan, China) for 20 mins at room temperature. .. Then, the sections were incubated with TXNIP (1:100, Abcam, Cambridge, MA, USA), NLRP3 (1:200, Abcam, Cambridge, MA, USA), and IL-1β (1:150, R & D, Minneapolis, MN, USA) primary antibodies at 4°C overnight and subsequently incubated at room temperature for 30 mins with an HRP-conjugated secondary antibody (Dako, Glostrup, Denmark), followed by staining with a diaminobenzidine (DAB) solution (Dako, Glostrup, Denmark).

    Article Title: Alteration of Podocyte Protein Expression and Localization in the Early Stage of Various Hemodynamic Conditions
    Article Snippet: .. After washing in phosphate buffered saline (PBS), the sections were repaired with sodium citrate buffer liquid at high pressure and incubated with PBS containing 5% normal bovine serum (Boster, Wuhan, China) for 1 h at 37 °C, followed by incubation in the primary antibodies at 4 °C overnight and corresponding secondary antibodies at 37 °C for 1 h. Then, the sections were incubated with a horseradish peroxidase (HRP)-conjugated avidin-biotin complex (ABC) for 20 min, and the staining was visualized with metal-enhanced 3,3′-diaminobenzidine (DAB) (Boster, Wuhan, China) for 5 min (ABC-DAB method). .. Finally, they were sealed with peucine and photographed under a light microscope or a confocal laser scanning microscope (FV10-ASW2.1Viewer).

    Article Title: SIP1 serves a role in HBx-induced liver cancer growth and metastasis
    Article Snippet: .. Immunofluorescent staining Following rinsing with phosphate-buffered saline (PBS), the cells were fixed on coverslips with 4% paraformaldehyde, permeabilized with 0.5% Triton X-100, blocked with goat serum (Boster Biological Technology, Ltd., Wuhan, China; cat. no. AR0009) and subsequently incubated with the indicated primary antibody (anti-E-cadherin or anti-vimentin; 1:100 dilution) overnight at 4°C. .. Following washing three times, the cells were incubated with DyLight 549-Conjugated Goat anti-mouse secondary antibody (Abbkine Scientific Co., Ltd.; cat. no. A23310; 1:100 dilution) or DyLight 549-Conjugated Goat Anti-Rabbit Secondary Antibody (Abbkine Scientific Co., Ltd.; cat. no. A23320; 1:100 dilution) at 37°C for 1 h. The nuclei of the cells were stained with 10 µ g/ml DAPI at 37°C for 10 min.

    Article Title: Sema7A, a brain immune regulator, regulates seizure activity in PTZ‐kindled epileptic rats, et al. Sema7A, a brain immune regulator, regulates seizure activity in PTZ‐kindled epileptic rats
    Article Snippet: .. After being washed with phosphate‐buffered saline (PBS) three times, the sections were blocked in 5% goat serum (Boster Biological Technology) for 1 hour at room temperature and then incubated with a mixture of a rabbit polyclonal anti‐Sema7A antibody (1:50, GeneTex) and an anti‐neuron‐specific enolase (NSE) mouse monoclonal antibody (1:50, Proteintech) or with a mixture of a rabbit polyclonal anti‐Sema7A antibody (1:50) and mouse monoclonal anti‐glial fibrillary acidic protein (GFAP) antibody (1:100, Boster Biological Technology) at 4°C overnight. .. The sections were washed with PBS and incubated with a mixture of fluorescein isothiocyanate (FITC)‐conjugated goat anti‐rabbit IgG (1:100, Beyotime) and Cy3‐labeled goat anti‐mouse IgG (1:200, Beyotime, Shanghai, China) in the dark for 60 minutes at 37°C.

    other:

    Article Title: A Dual-Modality MR/PA Imaging Contrast Agent Based on Ultrasmall Biopolymer Nanoparticles for Orthotopic Hepatocellular Carcinoma Imaging
    Article Snippet: Gadolinium(III)chloride hexahydrate (GdCl3 ·6H2 O), sodium hydroxide (NaOH) and hydrochloric acid (37 wt% HCl) were acquired from Sinopharm Chemical Reagent Beijing Co., Ltd. Amine-PEG5000 -amine (NH2 -PEG5000 -NH2 , 5 kDa) was obtained from Shanghai Zzbio Co., Ltd. Phosphate-buffered saline (PBS) and 4,6-diamidino-2-phenylindole (DAPI) was purchased from Boster biological Technology Co., Ltd. Fluorescein isothiocyanate were acquired from ACROS (90%, FITC).

    Activity Assay:

    Article Title: Angelica sinensis polysaccharide prevents mitochondrial apoptosis by regulating the Treg/Th17 ratio in aplastic anemia
    Article Snippet: .. A functional mitochondria isolation kit, mitochondrial outer membrane integrity testing kit, mitochondrial inner membrane integrity testing kit, purified mitochondrial cytochrome C oxidase activity assay kit, reactive oxygen species (ROS) assay kit and phosphate-buffered saline (PBS) were obtained from Wuhan Boster Biotechnology, Ltd. (Wuhan, China). .. The cell lysis buffer and horseradish peroxidase-conjugated secondary antibodies used for Western blotting were obtained from Beyotime Biotechnology (Shanghai, China).

    Staining:

    Article Title: Alteration of Podocyte Protein Expression and Localization in the Early Stage of Various Hemodynamic Conditions
    Article Snippet: .. After washing in phosphate buffered saline (PBS), the sections were repaired with sodium citrate buffer liquid at high pressure and incubated with PBS containing 5% normal bovine serum (Boster, Wuhan, China) for 1 h at 37 °C, followed by incubation in the primary antibodies at 4 °C overnight and corresponding secondary antibodies at 37 °C for 1 h. Then, the sections were incubated with a horseradish peroxidase (HRP)-conjugated avidin-biotin complex (ABC) for 20 min, and the staining was visualized with metal-enhanced 3,3′-diaminobenzidine (DAB) (Boster, Wuhan, China) for 5 min (ABC-DAB method). .. Finally, they were sealed with peucine and photographed under a light microscope or a confocal laser scanning microscope (FV10-ASW2.1Viewer).

    Article Title: SIP1 serves a role in HBx-induced liver cancer growth and metastasis
    Article Snippet: .. Immunofluorescent staining Following rinsing with phosphate-buffered saline (PBS), the cells were fixed on coverslips with 4% paraformaldehyde, permeabilized with 0.5% Triton X-100, blocked with goat serum (Boster Biological Technology, Ltd., Wuhan, China; cat. no. AR0009) and subsequently incubated with the indicated primary antibody (anti-E-cadherin or anti-vimentin; 1:100 dilution) overnight at 4°C. .. Following washing three times, the cells were incubated with DyLight 549-Conjugated Goat anti-mouse secondary antibody (Abbkine Scientific Co., Ltd.; cat. no. A23310; 1:100 dilution) or DyLight 549-Conjugated Goat Anti-Rabbit Secondary Antibody (Abbkine Scientific Co., Ltd.; cat. no. A23320; 1:100 dilution) at 37°C for 1 h. The nuclei of the cells were stained with 10 µ g/ml DAPI at 37°C for 10 min.

    Purification:

    Article Title: Angelica sinensis polysaccharide prevents mitochondrial apoptosis by regulating the Treg/Th17 ratio in aplastic anemia
    Article Snippet: .. A functional mitochondria isolation kit, mitochondrial outer membrane integrity testing kit, mitochondrial inner membrane integrity testing kit, purified mitochondrial cytochrome C oxidase activity assay kit, reactive oxygen species (ROS) assay kit and phosphate-buffered saline (PBS) were obtained from Wuhan Boster Biotechnology, Ltd. (Wuhan, China). .. The cell lysis buffer and horseradish peroxidase-conjugated secondary antibodies used for Western blotting were obtained from Beyotime Biotechnology (Shanghai, China).

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    • mouse anti vimentin antibody solution  (Boster Bio)
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    Boster Bio mouse anti vimentin antibody solution
    Expression of cell-specific marker in somatic cells derived from ear skin of buffalo. a Immunofluorescent staining of <t>vimentin</t> for fibroblasts, b negative control for vimentin (50 µm)
    Mouse Anti Vimentin Antibody Solution, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1 article reviews
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    pancreatic tissues fresh pancreatic tissue  (Boster Bio)
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    Boster Bio pancreatic tissues fresh pancreatic tissue
    Nuclear factor kappa-light-chain-enhancer of activated B cells and transforming growth factor beta expression in <t>pancreatic</t> <t>tissues.</t> A: Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and transforming growth factor beta (TGF-β) expression in pancreatic acinar cells and inflammatory cells; B: Immunohistochemistry assay for NF-κB expression; C: Immunohistochemistry assay for TGF-β expression. Negative: Blue; Positive: Yellow-brown. The results are presented as the mean ± SD. a P
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    phosphate buffer solution pbs  (Boster Bio)
    99
    Boster Bio phosphate buffer solution pbs
    Nuclear factor kappa-light-chain-enhancer of activated B cells and transforming growth factor beta expression in <t>pancreatic</t> <t>tissues.</t> A: Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and transforming growth factor beta (TGF-β) expression in pancreatic acinar cells and inflammatory cells; B: Immunohistochemistry assay for NF-κB expression; C: Immunohistochemistry assay for TGF-β expression. Negative: Blue; Positive: Yellow-brown. The results are presented as the mean ± SD. a P
    Phosphate Buffer Solution Pbs, supplied by Boster Bio, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffer solution pbs/product/Boster Bio
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    phosphate buffer solution pbs - by Bioz Stars, 2021-01
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Expression of cell-specific marker in somatic cells derived from ear skin of buffalo. a Immunofluorescent staining of vimentin for fibroblasts, b negative control for vimentin (50 µm)

    Journal: Cytotechnology

    Article Title: A comparative study on efficiency of adult fibroblasts and amniotic fluid-derived stem cells as donor cells for production of hand-made cloned buffalo (Bubalus bubalis) embryos

    doi: 10.1007/s10616-014-9805-1

    Figure Lengend Snippet: Expression of cell-specific marker in somatic cells derived from ear skin of buffalo. a Immunofluorescent staining of vimentin for fibroblasts, b negative control for vimentin (50 µm)

    Article Snippet: Samples were then treated with an acetone–methanol (1:1) solution for 15–20 min at room temperature and incubated in a penetrant solution (PBS with 1 % BSA and 0.1 % Tween-20) for 1 h. Subsequently, these were incubated in mouse anti-vimentin antibody solution (1:200 diluted with PBS; BM0135, BOSTER, Pleasanton, CA, USA) and mouse anti-keratin antibody solution (1:200 diluted with PBS; BM0030, BOSTER) overnight at 4 °C.

    Techniques: Expressing, Marker, Derivative Assay, Staining, Negative Control

    Nuclear factor kappa-light-chain-enhancer of activated B cells and transforming growth factor beta expression in pancreatic tissues. A: Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and transforming growth factor beta (TGF-β) expression in pancreatic acinar cells and inflammatory cells; B: Immunohistochemistry assay for NF-κB expression; C: Immunohistochemistry assay for TGF-β expression. Negative: Blue; Positive: Yellow-brown. The results are presented as the mean ± SD. a P

    Journal: World Journal of Gastroenterology

    Article Title: Sheng-jiang powder ameliorates obesity-induced pancreatic inflammatory injury via stimulating activation of the AMPK signalling pathway in rats

    doi: 10.3748/wjg.v24.i39.4448

    Figure Lengend Snippet: Nuclear factor kappa-light-chain-enhancer of activated B cells and transforming growth factor beta expression in pancreatic tissues. A: Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and transforming growth factor beta (TGF-β) expression in pancreatic acinar cells and inflammatory cells; B: Immunohistochemistry assay for NF-κB expression; C: Immunohistochemistry assay for TGF-β expression. Negative: Blue; Positive: Yellow-brown. The results are presented as the mean ± SD. a P

    Article Snippet: Histopathological analysis of pancreatic tissues Fresh pancreatic tissue samples were fixed with 40 g/L paraformaldehyde (AR1068, BOSTER, Wuhan, China), embedded in paraffin, sectioned into 5 μm sections, and stained with haematoxylin and eosin.

    Techniques: Expressing, Immunohistochemistry

    Histological images and pathologic scores of pancreatic tissues from the three experimental groups. A: Pathological images of the pancreatic tissues (× 200); B: Histological scores of the pancreatic tissues. The results are presented as the mean ± SD. a P

    Journal: World Journal of Gastroenterology

    Article Title: Sheng-jiang powder ameliorates obesity-induced pancreatic inflammatory injury via stimulating activation of the AMPK signalling pathway in rats

    doi: 10.3748/wjg.v24.i39.4448

    Figure Lengend Snippet: Histological images and pathologic scores of pancreatic tissues from the three experimental groups. A: Pathological images of the pancreatic tissues (× 200); B: Histological scores of the pancreatic tissues. The results are presented as the mean ± SD. a P

    Article Snippet: Histopathological analysis of pancreatic tissues Fresh pancreatic tissue samples were fixed with 40 g/L paraformaldehyde (AR1068, BOSTER, Wuhan, China), embedded in paraffin, sectioned into 5 μm sections, and stained with haematoxylin and eosin.

    Techniques: