ROS Assay:Article Title: Angelica sinensis polysaccharide prevents mitochondrial apoptosis by regulating the Treg/Th17 ratio in aplastic anemia
Article Snippet: .. A functional mitochondria isolation kit, mitochondrial outer membrane integrity testing kit, mitochondrial inner membrane integrity testing kit, purified mitochondrial cytochrome C oxidase activity assay kit, reactive oxygen species (ROS) assay kit and phosphate-buffered saline (PBS) were obtained from Wuhan Boster Biotechnology, Ltd. (Wuhan, China). .. The cell lysis buffer and horseradish peroxidase-conjugated secondary antibodies used for Western blotting were obtained from Beyotime Biotechnology (Shanghai, China).
Functional Assay:Article Title: Angelica sinensis polysaccharide prevents mitochondrial apoptosis by regulating the Treg/Th17 ratio in aplastic anemia
Article Snippet: .. A functional mitochondria isolation kit, mitochondrial outer membrane integrity testing kit, mitochondrial inner membrane integrity testing kit, purified mitochondrial cytochrome C oxidase activity assay kit, reactive oxygen species (ROS) assay kit and phosphate-buffered saline (PBS) were obtained from Wuhan Boster Biotechnology, Ltd. (Wuhan, China). .. The cell lysis buffer and horseradish peroxidase-conjugated secondary antibodies used for Western blotting were obtained from Beyotime Biotechnology (Shanghai, China).
Isolation:Article Title: Angelica sinensis polysaccharide prevents mitochondrial apoptosis by regulating the Treg/Th17 ratio in aplastic anemia
Article Snippet: .. A functional mitochondria isolation kit, mitochondrial outer membrane integrity testing kit, mitochondrial inner membrane integrity testing kit, purified mitochondrial cytochrome C oxidase activity assay kit, reactive oxygen species (ROS) assay kit and phosphate-buffered saline (PBS) were obtained from Wuhan Boster Biotechnology, Ltd. (Wuhan, China). .. The cell lysis buffer and horseradish peroxidase-conjugated secondary antibodies used for Western blotting were obtained from Beyotime Biotechnology (Shanghai, China).
Mouse Assay:Article Title: Short hairpin RNA attenuates liver fibrosis by regulating the PPAR-γ and NF-κB pathways in HBV-induced liver fibrosis in mice
Article Snippet: .. At the end of the experiment, mice were anesthetized with 2.5% avertin and perfused with cold PBS (pH 7.4) transcardially, followed by 4% paraformaldehyde (over 2 h at 25°C) in PBS (0.1 M, pH 7.4) (Boster Biological Technology Co., Ltd.) to fix tissues for immunohistochemistry (IHC). .. Livers were then collected for IHC analysis.
Immunohistochemistry:Article Title: Short hairpin RNA attenuates liver fibrosis by regulating the PPAR-γ and NF-κB pathways in HBV-induced liver fibrosis in mice
Article Snippet: .. At the end of the experiment, mice were anesthetized with 2.5% avertin and perfused with cold PBS (pH 7.4) transcardially, followed by 4% paraformaldehyde (over 2 h at 25°C) in PBS (0.1 M, pH 7.4) (Boster Biological Technology Co., Ltd.) to fix tissues for immunohistochemistry (IHC). .. Livers were then collected for IHC analysis.
Microscopy:Article Title: Association between FOXM1 and hedgehog signaling pathway in human cervical carcinoma by tissue microarray analysis
Article Snippet: .. Next, the slides were counterstained with hematoxylin (catalogue no. H9627, Sigma-Aldrich, St. Louis, MO, USA), rinsed several times with phosphate-buffered saline (PBS; Wuhan Boster Biological Technology, Ltd.) pH 7.4, dehydrated with a series of graded ethanol solutions, cleared using xylene (Sinopharm Group Co., Ltd.), and observed under an ECLIPSE E100 optical microscope (Nikon Corporation, Tokyo, Japan). .. To detect SHh and PTCH1 antigens, the endogenous peroxidase activity was blocked with rabbit serum (Shanghai Beiyi Bioequip Information Co., Ltd., Shanghai, China) for 30 min at room temperature, prior to incubating the tissue sections with the corresponding primary antibodies overnight at 4°C.
Avidin-Biotin Assay:Article Title: Alteration of Podocyte Protein Expression and Localization in the Early Stage of Various Hemodynamic Conditions
Article Snippet: .. After washing in phosphate buffered saline (PBS), the sections were repaired with sodium citrate buffer liquid at high pressure and incubated with PBS containing 5% normal bovine serum (Boster, Wuhan, China) for 1 h at 37 °C, followed by incubation in the primary antibodies at 4 °C overnight and corresponding secondary antibodies at 37 °C for 1 h. Then, the sections were incubated with a horseradish peroxidase (HRP)-conjugated avidin-biotin complex (ABC) for 20 min, and the staining was visualized with metal-enhanced 3,3′-diaminobenzidine (DAB) (Boster, Wuhan, China) for 5 min (ABC-DAB method). .. Finally, they were sealed with peucine and photographed under a light microscope or a confocal laser scanning microscope (FV10-ASW2.1Viewer).
Incubation:Article Title: Jinmaitong Ameliorates Diabetic Peripheral Neuropathy Through Suppressing TXNIP/NLRP3 Inflammasome Activation In The Streptozotocin-Induced Diabetic Rat Model
Article Snippet: .. After washing with phosphate-buffered saline with Tween-20 (PBST, pH 7.4), the sections were incubated in 3% H2 O2 solution and blocked with 10% normal goat serum (Boster, Wuhan, China) for 20 mins at room temperature. .. Then, the sections were incubated with TXNIP (1:100, Abcam, Cambridge, MA, USA), NLRP3 (1:200, Abcam, Cambridge, MA, USA), and IL-1β (1:150, R & D, Minneapolis, MN, USA) primary antibodies at 4°C overnight and subsequently incubated at room temperature for 30 mins with an HRP-conjugated secondary antibody (Dako, Glostrup, Denmark), followed by staining with a diaminobenzidine (DAB) solution (Dako, Glostrup, Denmark).
Article Title: Alteration of Podocyte Protein Expression and Localization in the Early Stage of Various Hemodynamic Conditions
Article Snippet: .. After washing in phosphate buffered saline (PBS), the sections were repaired with sodium citrate buffer liquid at high pressure and incubated with PBS containing 5% normal bovine serum (Boster, Wuhan, China) for 1 h at 37 °C, followed by incubation in the primary antibodies at 4 °C overnight and corresponding secondary antibodies at 37 °C for 1 h. Then, the sections were incubated with a horseradish peroxidase (HRP)-conjugated avidin-biotin complex (ABC) for 20 min, and the staining was visualized with metal-enhanced 3,3′-diaminobenzidine (DAB) (Boster, Wuhan, China) for 5 min (ABC-DAB method). .. Finally, they were sealed with peucine and photographed under a light microscope or a confocal laser scanning microscope (FV10-ASW2.1Viewer).
Article Title: SIP1 serves a role in HBx-induced liver cancer growth and metastasis
Article Snippet: .. Immunofluorescent staining Following rinsing with phosphate-buffered saline (PBS), the cells were fixed on coverslips with 4% paraformaldehyde, permeabilized with 0.5% Triton X-100, blocked with goat serum (Boster Biological Technology, Ltd., Wuhan, China; cat. no. AR0009) and subsequently incubated with the indicated primary antibody (anti-E-cadherin or anti-vimentin; 1:100 dilution) overnight at 4°C. .. Following washing three times, the cells were incubated with DyLight 549-Conjugated Goat anti-mouse secondary antibody (Abbkine Scientific Co., Ltd.; cat. no. A23310; 1:100 dilution) or DyLight 549-Conjugated Goat Anti-Rabbit Secondary Antibody (Abbkine Scientific Co., Ltd.; cat. no. A23320; 1:100 dilution) at 37°C for 1 h. The nuclei of the cells were stained with 10 µ g/ml DAPI at 37°C for 10 min.
Article Title: Sema7A, a brain immune regulator, regulates seizure activity in PTZ‐kindled epileptic rats, et al. Sema7A, a brain immune regulator, regulates seizure activity in PTZ‐kindled epileptic rats
Article Snippet: .. After being washed with phosphate‐buffered saline (PBS) three times, the sections were blocked in 5% goat serum (Boster Biological Technology) for 1 hour at room temperature and then incubated with a mixture of a rabbit polyclonal anti‐Sema7A antibody (1:50, GeneTex) and an anti‐neuron‐specific enolase (NSE) mouse monoclonal antibody (1:50, Proteintech) or with a mixture of a rabbit polyclonal anti‐Sema7A antibody (1:50) and mouse monoclonal anti‐glial fibrillary acidic protein (GFAP) antibody (1:100, Boster Biological Technology) at 4°C overnight. .. The sections were washed with PBS and incubated with a mixture of fluorescein isothiocyanate (FITC)‐conjugated goat anti‐rabbit IgG (1:100, Beyotime) and Cy3‐labeled goat anti‐mouse IgG (1:200, Beyotime, Shanghai, China) in the dark for 60 minutes at 37°C.
other:Article Title: A Dual-Modality MR/PA Imaging Contrast Agent Based on Ultrasmall Biopolymer Nanoparticles for Orthotopic Hepatocellular Carcinoma Imaging
Article Snippet: Gadolinium(III)chloride hexahydrate (GdCl3 ·6H2 O), sodium hydroxide (NaOH) and hydrochloric acid (37 wt% HCl) were acquired from Sinopharm Chemical Reagent Beijing Co., Ltd. Amine-PEG5000 -amine (NH2 -PEG5000 -NH2 , 5 kDa) was obtained from Shanghai Zzbio Co., Ltd. Phosphate-buffered saline (PBS) and 4,6-diamidino-2-phenylindole (DAPI) was purchased from Boster biological Technology Co., Ltd. Fluorescein isothiocyanate were acquired from ACROS (90%, FITC).
Activity Assay:Article Title: Angelica sinensis polysaccharide prevents mitochondrial apoptosis by regulating the Treg/Th17 ratio in aplastic anemia
Article Snippet: .. A functional mitochondria isolation kit, mitochondrial outer membrane integrity testing kit, mitochondrial inner membrane integrity testing kit, purified mitochondrial cytochrome C oxidase activity assay kit, reactive oxygen species (ROS) assay kit and phosphate-buffered saline (PBS) were obtained from Wuhan Boster Biotechnology, Ltd. (Wuhan, China). .. The cell lysis buffer and horseradish peroxidase-conjugated secondary antibodies used for Western blotting were obtained from Beyotime Biotechnology (Shanghai, China).
Staining:Article Title: Alteration of Podocyte Protein Expression and Localization in the Early Stage of Various Hemodynamic Conditions
Article Snippet: .. After washing in phosphate buffered saline (PBS), the sections were repaired with sodium citrate buffer liquid at high pressure and incubated with PBS containing 5% normal bovine serum (Boster, Wuhan, China) for 1 h at 37 °C, followed by incubation in the primary antibodies at 4 °C overnight and corresponding secondary antibodies at 37 °C for 1 h. Then, the sections were incubated with a horseradish peroxidase (HRP)-conjugated avidin-biotin complex (ABC) for 20 min, and the staining was visualized with metal-enhanced 3,3′-diaminobenzidine (DAB) (Boster, Wuhan, China) for 5 min (ABC-DAB method). .. Finally, they were sealed with peucine and photographed under a light microscope or a confocal laser scanning microscope (FV10-ASW2.1Viewer).
Article Title: SIP1 serves a role in HBx-induced liver cancer growth and metastasis
Article Snippet: .. Immunofluorescent staining Following rinsing with phosphate-buffered saline (PBS), the cells were fixed on coverslips with 4% paraformaldehyde, permeabilized with 0.5% Triton X-100, blocked with goat serum (Boster Biological Technology, Ltd., Wuhan, China; cat. no. AR0009) and subsequently incubated with the indicated primary antibody (anti-E-cadherin or anti-vimentin; 1:100 dilution) overnight at 4°C. .. Following washing three times, the cells were incubated with DyLight 549-Conjugated Goat anti-mouse secondary antibody (Abbkine Scientific Co., Ltd.; cat. no. A23310; 1:100 dilution) or DyLight 549-Conjugated Goat Anti-Rabbit Secondary Antibody (Abbkine Scientific Co., Ltd.; cat. no. A23320; 1:100 dilution) at 37°C for 1 h. The nuclei of the cells were stained with 10 µ g/ml DAPI at 37°C for 10 min.
Purification:Article Title: Angelica sinensis polysaccharide prevents mitochondrial apoptosis by regulating the Treg/Th17 ratio in aplastic anemia
Article Snippet: .. A functional mitochondria isolation kit, mitochondrial outer membrane integrity testing kit, mitochondrial inner membrane integrity testing kit, purified mitochondrial cytochrome C oxidase activity assay kit, reactive oxygen species (ROS) assay kit and phosphate-buffered saline (PBS) were obtained from Wuhan Boster Biotechnology, Ltd. (Wuhan, China). .. The cell lysis buffer and horseradish peroxidase-conjugated secondary antibodies used for Western blotting were obtained from Beyotime Biotechnology (Shanghai, China).
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