phosphate buffered saline pbs solution  (Bio-Rad)

 
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    Name:
    Phosphate Buffered Saline PBS
    Description:
    11 34 g makes 1 L reconstituted buffer
    Catalog Number:
    31098
    Price:
    None
    Category:
    Systemic Autoimmune Testing
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    Structured Review

    Bio-Rad phosphate buffered saline pbs solution
    Phosphate Buffered Saline PBS
    11 34 g makes 1 L reconstituted buffer
    https://www.bioz.com/result/phosphate buffered saline pbs solution/product/Bio-Rad
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    phosphate buffered saline pbs solution - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Red blood cells are dynamic reservoirs of cytokines
    Article Snippet: .. Binding of recombinant cytokines to RBCs Bio-Plex recombinant cytokine standards (27-plex and 21-plex human cytokine panels, Bio-Rad, Hercules, CA) contain cytokines at variable concentrations so these were reconstituted and diluted in PBS according to manufacturers instructions to produce standard-3 (27-plex and 21-plex human cytokine panels, Bio-Rad). .. Bio-Plex standard-3 was then diluted 1:8 in PBS with RBCs at a final concentration of 400 × 106 cells/mL and the cell suspension was incubated for 24 hours (37 °C).

    Purification:

    Article Title: Mapping Protective Regions on a Three-Dimensional Model of the Moraxella catarrhalis Vaccine Antigen Oligopeptide Permease A
    Article Snippet: .. In brief, 1 mg of His-tagged OppA was purified, buffer exchanged into 1 ml of 1× PBS (Bio-Rad, Hercules, CA), and sent to Covance (Denver, PA) to perform a 59-day immunization protocol. .. Each of 2 animals was given an initial subcutaneous injection of 250 μg OppA emulsified (1:1 [vol/vol]) in complete Freund's adjuvant.

    Article Title: LidA, a Translocated Substrate of the Legionella pneumophila Type IV Secretion System, Interferes with the Early Secretory Pathway
    Article Snippet: .. Sixty microliters of postexponential L. pneumophila expressing green fluorescent protein (GFP) was washed once and resuspended in 5 ml of phosphate-buffered saline (PBS) containing either 1% Triton X-100 (Bio-Rad), 1% CHAPS {3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate; Sigma}, 1% octylglucoside (Calbiochem), or 1% purified digitonin (Calbiochem) and incubated for 20 min at room temperature with intermittent vortexing. ..

    Incubation:

    Article Title: LidA, a Translocated Substrate of the Legionella pneumophila Type IV Secretion System, Interferes with the Early Secretory Pathway
    Article Snippet: .. Sixty microliters of postexponential L. pneumophila expressing green fluorescent protein (GFP) was washed once and resuspended in 5 ml of phosphate-buffered saline (PBS) containing either 1% Triton X-100 (Bio-Rad), 1% CHAPS {3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate; Sigma}, 1% octylglucoside (Calbiochem), or 1% purified digitonin (Calbiochem) and incubated for 20 min at room temperature with intermittent vortexing. ..

    Article Title: The development and use of an ELISA-based method to follow the distribution of cellulase monocomponents during the hydrolysis of pretreated corn stover
    Article Snippet: .. Following another washing step, the third antibody, a commercial GAR-AP (Bio-Rad) diluted in PBS with 1% (w/v) BSA, was added to the wells and incubated for another hour. .. After a final washing step, 35 mg/ml of p -nitrophenylphosphate (Bio-Rad), a substrate for alkaline phosphatase (AP), was added to the wells and the plate was incubated at room temperature for 30 minutes or until sufficient colour had developed.

    Expressing:

    Article Title: LidA, a Translocated Substrate of the Legionella pneumophila Type IV Secretion System, Interferes with the Early Secretory Pathway
    Article Snippet: .. Sixty microliters of postexponential L. pneumophila expressing green fluorescent protein (GFP) was washed once and resuspended in 5 ml of phosphate-buffered saline (PBS) containing either 1% Triton X-100 (Bio-Rad), 1% CHAPS {3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate; Sigma}, 1% octylglucoside (Calbiochem), or 1% purified digitonin (Calbiochem) and incubated for 20 min at room temperature with intermittent vortexing. ..

    Recombinant:

    Article Title: Red blood cells are dynamic reservoirs of cytokines
    Article Snippet: .. Bio-Plex recombinant cytokine standards (27-plex and 21-plex human cytokine panels, Bio-Rad, Hercules, CA) contain cytokines at variable concentrations so these were reconstituted and diluted in PBS according to manufacturers instructions to produce standard-3 (27-plex and 21-plex human cytokine panels, Bio-Rad). .. Bio-Plex standard-3 was then diluted 1:8 in PBS with RBCs at a final concentration of 400 × 106 cells/mL and the cell suspension was incubated for 24 hours (37 °C).

    Article Title: Red blood cells are dynamic reservoirs of cytokines
    Article Snippet: .. Binding of recombinant cytokines to RBCs Bio-Plex recombinant cytokine standards (27-plex and 21-plex human cytokine panels, Bio-Rad, Hercules, CA) contain cytokines at variable concentrations so these were reconstituted and diluted in PBS according to manufacturers instructions to produce standard-3 (27-plex and 21-plex human cytokine panels, Bio-Rad). .. Bio-Plex standard-3 was then diluted 1:8 in PBS with RBCs at a final concentration of 400 × 106 cells/mL and the cell suspension was incubated for 24 hours (37 °C).

    DC Protein Assay:

    Article Title: Confounding factors of ultrafiltration and protein analysis in extracellular vesicle research
    Article Snippet: .. A dilution series of 0 to 10% of Optiprep in PBS was obtained as a standard curve for performing absorbance readings with DC Protein assay (Bio-Rad, Hercules, California, USA), according to manufacturer’s instructions. ..

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  • 92
    Bio-Rad pbs solution
    a SDS-PAGE of blank LN homogenate, free dye, <t>bevacizumab</t> in <t>PBS</t> (2 μg), bevacizumab–800CW conjugate (2 μg) in PBS and bevacizumab–800CW conjugate (2 μg) spiked in blank mouse lymph node
    Pbs Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs solution/product/Bio-Rad
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    pbs solution - by Bioz Stars, 2020-08
    92/100 stars
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    93
    Bio-Rad alamarblue
    Knockdown of IGF1R enhanced the inhibitory efficacy of sorafenib in HCC cells by inhibiting AKT. (A) HCC cells were treated with sorafenib (1.25 μM for Hep3B, 2.5 μM for HepG2, and 5 μM for Huh7) for 24 hours. Expressions of p‐IGF1R, IGF1R, p‐AKT (ser473), AKT, p‐ERK, ERK, and GAPDH proteins were examined by western blotting. (B) Expressions of IGF1R, p‐AKT (ser473), AKT, p‐ERK, ERK, and GAPDH proteins were examined by western blotting in Hep3B cells infected with IGF1R shRNAs and scrambled shRNA lentiviral particles. (C) Cell proliferation was analyzed by the <t>alamarBlue</t> assay in Hep3B cells infected with IGF1R shRNAs and scrambled shRNA lentiviral particles and then treated with 1.25 μM sorafenib. (D) Expressions of IGF1R, AKT, and GAPDH proteins were examined by western blotting in Hep3B cells infected with scrambled shRNA, IGF1R shRNA, constitutively active AKT, or both lentiviral particles. (E) Cell proliferation was analyzed by the alamarBlue assay in IGF1R knockdown Hep3B cells infected with control or constitutively active AKT lentiviral particles and then treated with 1.25 μM sorafenib. Each experiment was repeated at least 3 times. Abbreviations: GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; RFU, relative fluorescence unit; shScr, short hairpin scrambled. Values in C and E were mean ± SD (n = 3 in each group).
    Alamarblue, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alamarblue/product/Bio-Rad
    Average 93 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    alamarblue - by Bioz Stars, 2020-08
    93/100 stars
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    92
    Bio-Rad sterile pbs solution
    Mice treated with 5 mg/kg of A6 compound exhibit a decreased lifespan and an increased PrP Sc burden. ( a ) Table summarizing the different groups of animals: prion inoculations, treatment performed, number of sick animals out of total number of animals (*means that in this group, one mouse was sacrificed while asymptomatic) and median survival time ± IQR (interquartile range). (b) Kaplan-Meier survival curves of mice intra-cerebrally (i.c.) inoculated with 5 μL of <t>22L</t> prions, and treated with 50 μL of DMSO (n = 9, blue) or with 5 mg/kg of A6 (n = 9, red) by intraperitoneal (i.p.) route, (+22L + DM versus +22L + A6–5: non–parametric Mantel-Cox log-rank test, ** p- value = 0,0067). Healthy control mice, inoculated with 5 μL of <t>PBS,</t> were treated with 5 mg/kg of A6 (n = 5, black dashed, −22L + A6–5) or non-treated (n = 5, brown, −22L + DM). (c) Left panel: PET-blots analysis of coronal sections of control healthy mice non-inoculated with prions and treated with 5 mg/kg of A6 (−22L + A6–5) (1) ; mice inoculated with 22L prions and either treated with DMSO (+22L + DM) (2) or treated with A6 (+22L + A6–5) (3) . Mice were all sacrificed at the same time (149, 149 and 147 dpi respectively) either in an asymptomatic or sick stage. Right panel: PET-blots analysis of coronal sections of sick mice: +22L + DM (4) and +22L + A6–5 (5) sacrificed at the same time (162 and 163 dpi, respectively). Sections were labelled with SAF84 antibody to detect PrP Sc and revealed with Vectastain ABC-AmP kit.
    Sterile Pbs Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sterile pbs solution/product/Bio-Rad
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    sterile pbs solution - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    a SDS-PAGE of blank LN homogenate, free dye, bevacizumab in PBS (2 μg), bevacizumab–800CW conjugate (2 μg) in PBS and bevacizumab–800CW conjugate (2 μg) spiked in blank mouse lymph node

    Journal: The AAPS Journal

    Article Title: Pharmacokinetics, Lymph Node Uptake, and Mechanistic PK Model of Near-Infrared Dye-Labeled Bevacizumab After IV and SC Administration in Mice

    doi: 10.1208/s12248-012-9342-9

    Figure Lengend Snippet: a SDS-PAGE of blank LN homogenate, free dye, bevacizumab in PBS (2 μg), bevacizumab–800CW conjugate (2 μg) in PBS and bevacizumab–800CW conjugate (2 μg) spiked in blank mouse lymph node

    Article Snippet: Briefly, 8 μL of 500 μg/mL of bevacizumab IRDye 800CW conjugate or 500 μg/mL of bevacizumab in PBS solution was mixed with 12 μL of sample buffer (Bio-Rad, 2% SDS w/v , 25% glycerol v/v , 0.001% bromophenol blue w/v , 62.5 mM Tris–HCl, pH 6.8) with 5% β-mercaptoethanol, and 10 μL of each mixture was loaded and separated on a 10% polyacrylamide gel.

    Techniques: SDS Page

    Knockdown of IGF1R enhanced the inhibitory efficacy of sorafenib in HCC cells by inhibiting AKT. (A) HCC cells were treated with sorafenib (1.25 μM for Hep3B, 2.5 μM for HepG2, and 5 μM for Huh7) for 24 hours. Expressions of p‐IGF1R, IGF1R, p‐AKT (ser473), AKT, p‐ERK, ERK, and GAPDH proteins were examined by western blotting. (B) Expressions of IGF1R, p‐AKT (ser473), AKT, p‐ERK, ERK, and GAPDH proteins were examined by western blotting in Hep3B cells infected with IGF1R shRNAs and scrambled shRNA lentiviral particles. (C) Cell proliferation was analyzed by the alamarBlue assay in Hep3B cells infected with IGF1R shRNAs and scrambled shRNA lentiviral particles and then treated with 1.25 μM sorafenib. (D) Expressions of IGF1R, AKT, and GAPDH proteins were examined by western blotting in Hep3B cells infected with scrambled shRNA, IGF1R shRNA, constitutively active AKT, or both lentiviral particles. (E) Cell proliferation was analyzed by the alamarBlue assay in IGF1R knockdown Hep3B cells infected with control or constitutively active AKT lentiviral particles and then treated with 1.25 μM sorafenib. Each experiment was repeated at least 3 times. Abbreviations: GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; RFU, relative fluorescence unit; shScr, short hairpin scrambled. Values in C and E were mean ± SD (n = 3 in each group).

    Journal: Hepatology Communications

    Article Title: Inhibition of insulin‐like growth factor 1 receptor enhances the efficacy of sorafenib in inhibiting hepatocellular carcinoma cell growth and survival

    doi: 10.1002/hep4.1181

    Figure Lengend Snippet: Knockdown of IGF1R enhanced the inhibitory efficacy of sorafenib in HCC cells by inhibiting AKT. (A) HCC cells were treated with sorafenib (1.25 μM for Hep3B, 2.5 μM for HepG2, and 5 μM for Huh7) for 24 hours. Expressions of p‐IGF1R, IGF1R, p‐AKT (ser473), AKT, p‐ERK, ERK, and GAPDH proteins were examined by western blotting. (B) Expressions of IGF1R, p‐AKT (ser473), AKT, p‐ERK, ERK, and GAPDH proteins were examined by western blotting in Hep3B cells infected with IGF1R shRNAs and scrambled shRNA lentiviral particles. (C) Cell proliferation was analyzed by the alamarBlue assay in Hep3B cells infected with IGF1R shRNAs and scrambled shRNA lentiviral particles and then treated with 1.25 μM sorafenib. (D) Expressions of IGF1R, AKT, and GAPDH proteins were examined by western blotting in Hep3B cells infected with scrambled shRNA, IGF1R shRNA, constitutively active AKT, or both lentiviral particles. (E) Cell proliferation was analyzed by the alamarBlue assay in IGF1R knockdown Hep3B cells infected with control or constitutively active AKT lentiviral particles and then treated with 1.25 μM sorafenib. Each experiment was repeated at least 3 times. Abbreviations: GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; RFU, relative fluorescence unit; shScr, short hairpin scrambled. Values in C and E were mean ± SD (n = 3 in each group).

    Article Snippet: At different time points, culture media was removed and alamarBlue (BUF012A; Bio‐Rad, Hercules, CA) solution (1:10 dilution in phosphate‐buffered saline) was added to the cells.

    Techniques: Western Blot, Infection, shRNA, Alamar Blue Assay, Fluorescence

    Ceritinib suppressed HCC cell growth by inhibiting the IGF1R/AKT pathway. (A) HCC cells were treated with ceritinib (0.5 μM for Hep3B, 1 μM for HepG2, and 2 μM for Huh7) for 24 hours. Expressions of p‐IGF1R, IGF1R, p‐AKT (ser473), AKT, p‐ERK, ERK, and GAPDH proteins were examined by western blotting. (B) HCC cells were treated with ceritinib at different doses for 48 hours. Cell proliferation was analyzed by the alamarBlue assay. (C) Cell proliferation was analyzed by the alamarBlue assay in Hep3B cells infected with IGF1R shRNAs and scrambled shRNA lentiviral particles. (D) Hep3B cells infected with control or constitutively active AKT lentiviral particles were treated with 0.5 μM ceritinib for 48 hours. Cells were then cultured for 14 days and stained with 0.5% crystal violet. (E) Cell proliferation was analyzed by the alamarBlue assay in IGF1R knockdown Hep3B cells infected with control or constitutively active AKT lentiviral particles. Each experiment was repeated at least 3 times. Abbreviations: DMSO, dimethyl sulfoxide; GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; RFU, relative fluorescence unit. Values in B, C, D, and E were mean ± SD (n = 3 in each group).

    Journal: Hepatology Communications

    Article Title: Inhibition of insulin‐like growth factor 1 receptor enhances the efficacy of sorafenib in inhibiting hepatocellular carcinoma cell growth and survival

    doi: 10.1002/hep4.1181

    Figure Lengend Snippet: Ceritinib suppressed HCC cell growth by inhibiting the IGF1R/AKT pathway. (A) HCC cells were treated with ceritinib (0.5 μM for Hep3B, 1 μM for HepG2, and 2 μM for Huh7) for 24 hours. Expressions of p‐IGF1R, IGF1R, p‐AKT (ser473), AKT, p‐ERK, ERK, and GAPDH proteins were examined by western blotting. (B) HCC cells were treated with ceritinib at different doses for 48 hours. Cell proliferation was analyzed by the alamarBlue assay. (C) Cell proliferation was analyzed by the alamarBlue assay in Hep3B cells infected with IGF1R shRNAs and scrambled shRNA lentiviral particles. (D) Hep3B cells infected with control or constitutively active AKT lentiviral particles were treated with 0.5 μM ceritinib for 48 hours. Cells were then cultured for 14 days and stained with 0.5% crystal violet. (E) Cell proliferation was analyzed by the alamarBlue assay in IGF1R knockdown Hep3B cells infected with control or constitutively active AKT lentiviral particles. Each experiment was repeated at least 3 times. Abbreviations: DMSO, dimethyl sulfoxide; GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; RFU, relative fluorescence unit. Values in B, C, D, and E were mean ± SD (n = 3 in each group).

    Article Snippet: At different time points, culture media was removed and alamarBlue (BUF012A; Bio‐Rad, Hercules, CA) solution (1:10 dilution in phosphate‐buffered saline) was added to the cells.

    Techniques: Western Blot, Alamar Blue Assay, Infection, shRNA, Cell Culture, Staining, Fluorescence

    Knockdown of IGF1R enhanced the inhibitory efficacy of sorafenib in HCC cells by inhibiting AKT. (A) HCC cells were treated with sorafenib (1.25 μM for Hep3B, 2.5 μM for HepG2, and 5 μM for Huh7) for 24 hours. Expressions of p‐IGF1R, IGF1R, p‐AKT (ser473), AKT, p‐ERK, ERK, and GAPDH proteins were examined by western blotting. (B) Expressions of IGF1R, p‐AKT (ser473), AKT, p‐ERK, ERK, and GAPDH proteins were examined by western blotting in Hep3B cells infected with IGF1R shRNAs and scrambled shRNA lentiviral particles. (C) Cell proliferation was analyzed by the alamarBlue assay in Hep3B cells infected with IGF1R shRNAs and scrambled shRNA lentiviral particles and then treated with 1.25 μM sorafenib. (D) Expressions of IGF1R, AKT, and GAPDH proteins were examined by western blotting in Hep3B cells infected with scrambled shRNA, IGF1R shRNA, constitutively active AKT, or both lentiviral particles. (E) Cell proliferation was analyzed by the alamarBlue assay in IGF1R knockdown Hep3B cells infected with control or constitutively active AKT lentiviral particles and then treated with 1.25 μM sorafenib. Each experiment was repeated at least 3 times. Abbreviations: GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; RFU, relative fluorescence unit; shScr, short hairpin scrambled. Values in C and E were mean ± SD (n = 3 in each group).

    Journal: Hepatology Communications

    Article Title: Inhibition of insulin‐like growth factor 1 receptor enhances the efficacy of sorafenib in inhibiting hepatocellular carcinoma cell growth and survival

    doi: 10.1002/hep4.1181

    Figure Lengend Snippet: Knockdown of IGF1R enhanced the inhibitory efficacy of sorafenib in HCC cells by inhibiting AKT. (A) HCC cells were treated with sorafenib (1.25 μM for Hep3B, 2.5 μM for HepG2, and 5 μM for Huh7) for 24 hours. Expressions of p‐IGF1R, IGF1R, p‐AKT (ser473), AKT, p‐ERK, ERK, and GAPDH proteins were examined by western blotting. (B) Expressions of IGF1R, p‐AKT (ser473), AKT, p‐ERK, ERK, and GAPDH proteins were examined by western blotting in Hep3B cells infected with IGF1R shRNAs and scrambled shRNA lentiviral particles. (C) Cell proliferation was analyzed by the alamarBlue assay in Hep3B cells infected with IGF1R shRNAs and scrambled shRNA lentiviral particles and then treated with 1.25 μM sorafenib. (D) Expressions of IGF1R, AKT, and GAPDH proteins were examined by western blotting in Hep3B cells infected with scrambled shRNA, IGF1R shRNA, constitutively active AKT, or both lentiviral particles. (E) Cell proliferation was analyzed by the alamarBlue assay in IGF1R knockdown Hep3B cells infected with control or constitutively active AKT lentiviral particles and then treated with 1.25 μM sorafenib. Each experiment was repeated at least 3 times. Abbreviations: GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; RFU, relative fluorescence unit; shScr, short hairpin scrambled. Values in C and E were mean ± SD (n = 3 in each group).

    Article Snippet: At different time points, culture media was removed and alamarBlue (BUF012A; Bio‐Rad, Hercules, CA) solution (1:10 dilution in phosphate‐buffered saline) was added to the cells.

    Techniques: Western Blot, Infection, shRNA, Alamar Blue Assay, Fluorescence

    Ceritinib suppressed HCC cell growth by inhibiting the IGF1R/AKT pathway. (A) HCC cells were treated with ceritinib (0.5 μM for Hep3B, 1 μM for HepG2, and 2 μM for Huh7) for 24 hours. Expressions of p‐IGF1R, IGF1R, p‐AKT (ser473), AKT, p‐ERK, ERK, and GAPDH proteins were examined by western blotting. (B) HCC cells were treated with ceritinib at different doses for 48 hours. Cell proliferation was analyzed by the alamarBlue assay. (C) Cell proliferation was analyzed by the alamarBlue assay in Hep3B cells infected with IGF1R shRNAs and scrambled shRNA lentiviral particles. (D) Hep3B cells infected with control or constitutively active AKT lentiviral particles were treated with 0.5 μM ceritinib for 48 hours. Cells were then cultured for 14 days and stained with 0.5% crystal violet. (E) Cell proliferation was analyzed by the alamarBlue assay in IGF1R knockdown Hep3B cells infected with control or constitutively active AKT lentiviral particles. Each experiment was repeated at least 3 times. Abbreviations: DMSO, dimethyl sulfoxide; GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; RFU, relative fluorescence unit. Values in B, C, D, and E were mean ± SD (n = 3 in each group).

    Journal: Hepatology Communications

    Article Title: Inhibition of insulin‐like growth factor 1 receptor enhances the efficacy of sorafenib in inhibiting hepatocellular carcinoma cell growth and survival

    doi: 10.1002/hep4.1181

    Figure Lengend Snippet: Ceritinib suppressed HCC cell growth by inhibiting the IGF1R/AKT pathway. (A) HCC cells were treated with ceritinib (0.5 μM for Hep3B, 1 μM for HepG2, and 2 μM for Huh7) for 24 hours. Expressions of p‐IGF1R, IGF1R, p‐AKT (ser473), AKT, p‐ERK, ERK, and GAPDH proteins were examined by western blotting. (B) HCC cells were treated with ceritinib at different doses for 48 hours. Cell proliferation was analyzed by the alamarBlue assay. (C) Cell proliferation was analyzed by the alamarBlue assay in Hep3B cells infected with IGF1R shRNAs and scrambled shRNA lentiviral particles. (D) Hep3B cells infected with control or constitutively active AKT lentiviral particles were treated with 0.5 μM ceritinib for 48 hours. Cells were then cultured for 14 days and stained with 0.5% crystal violet. (E) Cell proliferation was analyzed by the alamarBlue assay in IGF1R knockdown Hep3B cells infected with control or constitutively active AKT lentiviral particles. Each experiment was repeated at least 3 times. Abbreviations: DMSO, dimethyl sulfoxide; GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; RFU, relative fluorescence unit. Values in B, C, D, and E were mean ± SD (n = 3 in each group).

    Article Snippet: At different time points, culture media was removed and alamarBlue (BUF012A; Bio‐Rad, Hercules, CA) solution (1:10 dilution in phosphate‐buffered saline) was added to the cells.

    Techniques: Western Blot, Alamar Blue Assay, Infection, shRNA, Cell Culture, Staining, Fluorescence

    Mice treated with 5 mg/kg of A6 compound exhibit a decreased lifespan and an increased PrP Sc burden. ( a ) Table summarizing the different groups of animals: prion inoculations, treatment performed, number of sick animals out of total number of animals (*means that in this group, one mouse was sacrificed while asymptomatic) and median survival time ± IQR (interquartile range). (b) Kaplan-Meier survival curves of mice intra-cerebrally (i.c.) inoculated with 5 μL of 22L prions, and treated with 50 μL of DMSO (n = 9, blue) or with 5 mg/kg of A6 (n = 9, red) by intraperitoneal (i.p.) route, (+22L + DM versus +22L + A6–5: non–parametric Mantel-Cox log-rank test, ** p- value = 0,0067). Healthy control mice, inoculated with 5 μL of PBS, were treated with 5 mg/kg of A6 (n = 5, black dashed, −22L + A6–5) or non-treated (n = 5, brown, −22L + DM). (c) Left panel: PET-blots analysis of coronal sections of control healthy mice non-inoculated with prions and treated with 5 mg/kg of A6 (−22L + A6–5) (1) ; mice inoculated with 22L prions and either treated with DMSO (+22L + DM) (2) or treated with A6 (+22L + A6–5) (3) . Mice were all sacrificed at the same time (149, 149 and 147 dpi respectively) either in an asymptomatic or sick stage. Right panel: PET-blots analysis of coronal sections of sick mice: +22L + DM (4) and +22L + A6–5 (5) sacrificed at the same time (162 and 163 dpi, respectively). Sections were labelled with SAF84 antibody to detect PrP Sc and revealed with Vectastain ABC-AmP kit.

    Journal: Scientific Reports

    Article Title: Low doses of bioherbicide favour prion aggregation and propagation in vivo

    doi: 10.1038/s41598-018-25966-9

    Figure Lengend Snippet: Mice treated with 5 mg/kg of A6 compound exhibit a decreased lifespan and an increased PrP Sc burden. ( a ) Table summarizing the different groups of animals: prion inoculations, treatment performed, number of sick animals out of total number of animals (*means that in this group, one mouse was sacrificed while asymptomatic) and median survival time ± IQR (interquartile range). (b) Kaplan-Meier survival curves of mice intra-cerebrally (i.c.) inoculated with 5 μL of 22L prions, and treated with 50 μL of DMSO (n = 9, blue) or with 5 mg/kg of A6 (n = 9, red) by intraperitoneal (i.p.) route, (+22L + DM versus +22L + A6–5: non–parametric Mantel-Cox log-rank test, ** p- value = 0,0067). Healthy control mice, inoculated with 5 μL of PBS, were treated with 5 mg/kg of A6 (n = 5, black dashed, −22L + A6–5) or non-treated (n = 5, brown, −22L + DM). (c) Left panel: PET-blots analysis of coronal sections of control healthy mice non-inoculated with prions and treated with 5 mg/kg of A6 (−22L + A6–5) (1) ; mice inoculated with 22L prions and either treated with DMSO (+22L + DM) (2) or treated with A6 (+22L + A6–5) (3) . Mice were all sacrificed at the same time (149, 149 and 147 dpi respectively) either in an asymptomatic or sick stage. Right panel: PET-blots analysis of coronal sections of sick mice: +22L + DM (4) and +22L + A6–5 (5) sacrificed at the same time (162 and 163 dpi, respectively). Sections were labelled with SAF84 antibody to detect PrP Sc and revealed with Vectastain ABC-AmP kit.

    Article Snippet: Brains from a terminally sick mouse (infected with 22L prions) or from a non-infected mouse were homogenized in 10% (w/v) sterile PBS solution using microbead-containing tubes and a Ribolysor apparatus (Biorad, Marnes la Coquette, France).

    Techniques: Mouse Assay, Positron Emission Tomography