phosphate buffer saline pbs  (Millipore)

 
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    Name:
    Phosphate Buffered Saline
    Description:
    Phosphate Buffered Saline PBS is routinely used as a wash buffer in Western blot and immunoprecipitation IP procedures as well as during sample preparation of cell lysates for use in general molecular biology applications
    Catalog Number:
    p7059
    Price:
    None
    Applications:
    Suitable for use in wash buffers for Western blots and IP, as well as cell lysate sample preparation.
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    Structured Review

    Millipore phosphate buffer saline pbs
    Phosphate Buffered Saline PBS is routinely used as a wash buffer in Western blot and immunoprecipitation IP procedures as well as during sample preparation of cell lysates for use in general molecular biology applications
    https://www.bioz.com/result/phosphate buffer saline pbs/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphate buffer saline pbs - by Bioz Stars, 2021-03
    97/100 stars

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    Injection:

    Article Title: Type II Collagen Induces Peripheral Tolerance in BALB/c Mice via the Generation of CD8+ T Regulatory Cells
    Article Snippet: BALB/c mice were anesthetized by isoflurane anesthesia (2–3% isoflurane with oxygen supply). .. About 50–100 µg of CII in PBS (Phosphate-Buffered Saline) was injected (in 5 µl; Sigma, St. Louis, MO) into the AC of the eye. .. Mice receiving PBS alone via intracameral injection (in the AC of the eye) were used as controls.

    other:

    Article Title: Fabrication and Optimization of Bilayered Nanoporous Anodic Alumina Structures as Multi-Point Interferometric Sensing Platform
    Article Snippet: Oxalic acid (C2 H2 O4 ), perchloric acid (HClO4 ), phosphoric acid (H3 PO4 ), chromic acid (H2 CrO4 ), (3-aminopropyl)trimethoxysilane (APTES), hydrogen peroxide (H2 O2 ), glutaraldehyde (CH2 (CH2 CHO)2 —GTA), sodium hydroxide (NaOH), human serum albumin (HSA), quercetin (C15 H10 O7 ), and phosphate buffer saline (PBS) were purchased from Sigma-Aldrich (Australia).

    Mouse Assay:

    Article Title: Suppression of Nicotine-Induced Pathophysiology by an Adenovirus Hexon-Based Antinicotine Vaccine
    Article Snippet: .. To determine the effect of the vaccine on mice sensitized to nicotine, nonvaccinated naive C57BL/6 male mice ( n =16/group) were administered either saline (PBS) or nicotine [(−) nicotine hydrogen tartrate (Sigma), 200 μL, 0.5 mg/kg wt] subcutaneously in nape of neck, daily, 10 times over the course of 2 weeks ( ). .. The mice were monitored for nicotine-induced changes in activity using infrared beam–equipped open field chambers (20×20 cm chamber, AccuScan Instruments, Columbus, OH).

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  • 97
    Millipore facs blocking buffer
    CR8-treatment triggers apoptosis in murine and human hepatic stellate cell lines. Murine GRX and human LX-2 cells were treated for 48 h with increasing concentrations of CR8 as indicated. Dimethyl sulfoxide (DMSO) treatment alone (0 nM) served as control. ( a ) Schematic overview of the experimental design. ( b ) GRX (upper panels) and LX-2 (lower panels) cells were investigated by brightfield microscopy for cell density and morphology at a magnification of 100×. Arrows highlight round-shaped cells, indicative for cell death. ( c , d ) Cells were stained with fluorescence-labelled antibodies against cleaved poly (ADP-ribose)-polymerase 1 <t>(PARP-1)</t> and analyzed via flow cytometry, in particular fluorescence-associated cell sorting <t>(FACS).</t> ( c ) Representative FACS-plots of cleaved PARP-1 positive (% of single cells) GRX (upper panels) and LX-2 (lower panels) cells after treatment with increasing concentrations of CR8. ( d ) Quantification of cleaved PARP-1 positive GRX (left panel) and LX-2 (right panel) cells (% of single cells) from n = 6 independent FACS experiments. Data are shown as fold induction compared to controls. ( e ) Specific caspase-3 enzyme activity in GRX (left panel, n = 4) and LX-2 (right panel, n = 3) cells after CR8 treatment. Values are given as arbitrary fluorescence units (AFU)/μg protein/h and are calculated as fold induction in comparison to controls. Data reflect the mean of at least n = 3 independent experiments, unless otherwise indicated. * p
    Facs Blocking Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/facs blocking buffer/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    facs blocking buffer - by Bioz Stars, 2021-03
    97/100 stars
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    97
    Millipore pbs solution
    a) Electrical benchtop recording output from the Ag <t>NWs</t> microelectrodes, with 10 Hz, 20 mV peak-to-peak sine wave input. b) PSD of the signals recorded by the Ag NWs microelectrodes in (a). c) Soak test of the Ag NWs microelectrodes in a <t>PBS</t> solution at 37 °C. Z0 is the impedance at day 0, whereas Z represents the impedance at a specific day. d) CV curves of the Ag NWs microelectrodes at various scan rates.
    Pbs Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs solution/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbs solution - by Bioz Stars, 2021-03
    97/100 stars
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    97
    Millipore blue fluorescent nucleic acid stain dapi
    Morphology of the three main types of hemocytes in 5 th instar P. napi larvae: (A) plasmatocytes, (B) granulocytes, and (C) early and mature oenocytoids. Hemocytes of P. napi larvae after in vivo phagocytosis of E. coli (AlexaFluor488, green): (D) plasmatocyte (indicated by P ) and granulocytes (indicated by G ), (E) and early oenocytoid. (F) Staining of an oenocytoid with PPO antibody (green). Cell nuclei are stained with <t>DAPI</t> (blue) and the cytoskeleton of A,B,D,E F with <t>phalloidin</t> (red). Scalebar = 15 um. Images taken using epifluorescent microscope (C) and confocal microscope (A,B,D,E F).
    Blue Fluorescent Nucleic Acid Stain Dapi, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/blue fluorescent nucleic acid stain dapi/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    blue fluorescent nucleic acid stain dapi - by Bioz Stars, 2021-03
    97/100 stars
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    99
    Millipore fitc phalloidin
    Epifluorescence microscopy. M1 ( a ) and M2 ( b ) MDMs labelled with <t>FITC-phalloidin</t> to stain cytoskeletal actin (in green), and DAPI nuclear counterstain (in blue). Scale bar: 100 μm.
    Fitc Phalloidin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc phalloidin/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fitc phalloidin - by Bioz Stars, 2021-03
    99/100 stars
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    Image Search Results


    CR8-treatment triggers apoptosis in murine and human hepatic stellate cell lines. Murine GRX and human LX-2 cells were treated for 48 h with increasing concentrations of CR8 as indicated. Dimethyl sulfoxide (DMSO) treatment alone (0 nM) served as control. ( a ) Schematic overview of the experimental design. ( b ) GRX (upper panels) and LX-2 (lower panels) cells were investigated by brightfield microscopy for cell density and morphology at a magnification of 100×. Arrows highlight round-shaped cells, indicative for cell death. ( c , d ) Cells were stained with fluorescence-labelled antibodies against cleaved poly (ADP-ribose)-polymerase 1 (PARP-1) and analyzed via flow cytometry, in particular fluorescence-associated cell sorting (FACS). ( c ) Representative FACS-plots of cleaved PARP-1 positive (% of single cells) GRX (upper panels) and LX-2 (lower panels) cells after treatment with increasing concentrations of CR8. ( d ) Quantification of cleaved PARP-1 positive GRX (left panel) and LX-2 (right panel) cells (% of single cells) from n = 6 independent FACS experiments. Data are shown as fold induction compared to controls. ( e ) Specific caspase-3 enzyme activity in GRX (left panel, n = 4) and LX-2 (right panel, n = 3) cells after CR8 treatment. Values are given as arbitrary fluorescence units (AFU)/μg protein/h and are calculated as fold induction in comparison to controls. Data reflect the mean of at least n = 3 independent experiments, unless otherwise indicated. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Pharmacological Inhibition of Cyclin-Dependent Kinases Triggers Anti-Fibrotic Effects in Hepatic Stellate Cells In Vitro

    doi: 10.3390/ijms21093267

    Figure Lengend Snippet: CR8-treatment triggers apoptosis in murine and human hepatic stellate cell lines. Murine GRX and human LX-2 cells were treated for 48 h with increasing concentrations of CR8 as indicated. Dimethyl sulfoxide (DMSO) treatment alone (0 nM) served as control. ( a ) Schematic overview of the experimental design. ( b ) GRX (upper panels) and LX-2 (lower panels) cells were investigated by brightfield microscopy for cell density and morphology at a magnification of 100×. Arrows highlight round-shaped cells, indicative for cell death. ( c , d ) Cells were stained with fluorescence-labelled antibodies against cleaved poly (ADP-ribose)-polymerase 1 (PARP-1) and analyzed via flow cytometry, in particular fluorescence-associated cell sorting (FACS). ( c ) Representative FACS-plots of cleaved PARP-1 positive (% of single cells) GRX (upper panels) and LX-2 (lower panels) cells after treatment with increasing concentrations of CR8. ( d ) Quantification of cleaved PARP-1 positive GRX (left panel) and LX-2 (right panel) cells (% of single cells) from n = 6 independent FACS experiments. Data are shown as fold induction compared to controls. ( e ) Specific caspase-3 enzyme activity in GRX (left panel, n = 4) and LX-2 (right panel, n = 3) cells after CR8 treatment. Values are given as arbitrary fluorescence units (AFU)/μg protein/h and are calculated as fold induction in comparison to controls. Data reflect the mean of at least n = 3 independent experiments, unless otherwise indicated. * p

    Article Snippet: Co-staining of cells was performed using a mix of fluorescence-labeled antibodies against pH3-AL647 (1:50, Cell Signalling Technology, Danver, MA, USA) and cleaved PARP-PE (1:50, BD Biosciences, San Jose, CA, USA) diluted in FACS-blocking buffer (mixture of 0.66% human/rabbit/mouse serum, Sigma-Aldrich, and 1% Bovine Serum Albumin, Sigma-Aldrich in 1× PBS) for 30 min at 4 °C.

    Techniques: Microscopy, Staining, Fluorescence, Flow Cytometry, FACS, Activity Assay

    a) Electrical benchtop recording output from the Ag NWs microelectrodes, with 10 Hz, 20 mV peak-to-peak sine wave input. b) PSD of the signals recorded by the Ag NWs microelectrodes in (a). c) Soak test of the Ag NWs microelectrodes in a PBS solution at 37 °C. Z0 is the impedance at day 0, whereas Z represents the impedance at a specific day. d) CV curves of the Ag NWs microelectrodes at various scan rates.

    Journal: bioRxiv

    Article Title: Flexible and transparent silver nanowire structures for multifunctional electrical and optical biointerfacing

    doi: 10.1101/2020.10.10.334755

    Figure Lengend Snippet: a) Electrical benchtop recording output from the Ag NWs microelectrodes, with 10 Hz, 20 mV peak-to-peak sine wave input. b) PSD of the signals recorded by the Ag NWs microelectrodes in (a). c) Soak test of the Ag NWs microelectrodes in a PBS solution at 37 °C. Z0 is the impedance at day 0, whereas Z represents the impedance at a specific day. d) CV curves of the Ag NWs microelectrodes at various scan rates.

    Article Snippet: CV tests were measured within a potential window from −0.8 to 0.8 V. The electrochemical measurements used a three-electrode configuration in a 1 × PBS solution (Sigma-Aldrich), where the Ag NWs microelectrodes, a platinum electrode, and an Ag/AgCl electrode served as the working, counter, and reference electrodes, respectively.

    Techniques:

    Morphology of the three main types of hemocytes in 5 th instar P. napi larvae: (A) plasmatocytes, (B) granulocytes, and (C) early and mature oenocytoids. Hemocytes of P. napi larvae after in vivo phagocytosis of E. coli (AlexaFluor488, green): (D) plasmatocyte (indicated by P ) and granulocytes (indicated by G ), (E) and early oenocytoid. (F) Staining of an oenocytoid with PPO antibody (green). Cell nuclei are stained with DAPI (blue) and the cytoskeleton of A,B,D,E F with phalloidin (red). Scalebar = 15 um. Images taken using epifluorescent microscope (C) and confocal microscope (A,B,D,E F).

    Journal: bioRxiv

    Article Title: Geographic variation in hemocyte diversity and phagocytic propensity shows a diffuse genomic signature in the green veined white butterfly

    doi: 10.1101/790782

    Figure Lengend Snippet: Morphology of the three main types of hemocytes in 5 th instar P. napi larvae: (A) plasmatocytes, (B) granulocytes, and (C) early and mature oenocytoids. Hemocytes of P. napi larvae after in vivo phagocytosis of E. coli (AlexaFluor488, green): (D) plasmatocyte (indicated by P ) and granulocytes (indicated by G ), (E) and early oenocytoid. (F) Staining of an oenocytoid with PPO antibody (green). Cell nuclei are stained with DAPI (blue) and the cytoskeleton of A,B,D,E F with phalloidin (red). Scalebar = 15 um. Images taken using epifluorescent microscope (C) and confocal microscope (A,B,D,E F).

    Article Snippet: To distinguish morphological features and reveal the nuclei the cells were treated with Phalloidin Rhodamine (Biotium, 3:1000 dilution in PBS) together with blue-fluorescent nucleic acid stain DAPI (Sigma-Aldrich, 1:1000 dilution in PBS) for 10 minutes in darkness, RT.

    Techniques: In Vivo, Staining, Microscopy

    Epifluorescence microscopy. M1 ( a ) and M2 ( b ) MDMs labelled with FITC-phalloidin to stain cytoskeletal actin (in green), and DAPI nuclear counterstain (in blue). Scale bar: 100 μm.

    Journal: Scientific Reports

    Article Title: Classification of M1/M2-polarized human macrophages by label-free hyperspectral reflectance confocal microscopy and multivariate analysis

    doi: 10.1038/s41598-017-08121-8

    Figure Lengend Snippet: Epifluorescence microscopy. M1 ( a ) and M2 ( b ) MDMs labelled with FITC-phalloidin to stain cytoskeletal actin (in green), and DAPI nuclear counterstain (in blue). Scale bar: 100 μm.

    Article Snippet: After washing, cytoskeletal actin was labelled with FITC-phalloidin (Sigma-Aldrich, 1:400 in PBS for 45 min), and nuclei were counterstained with DAPI (Thermo Fisher Scientific, 1:10000 in PBS for 15 min).

    Techniques: Epifluorescence Microscopy, Staining