phosphate buffer saline pbs  (Beyotime)

 
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    Name:
    GTP|Guanosine 5 triphosphate sodium salt hydrate
    Description:

    Catalog Number:
    ST1362-25MG
    Price:
    62.0
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    Structured Review

    Beyotime phosphate buffer saline pbs
    Calibration of CNT@DNA-Mn 3 (PO 4 ) 2 modified glass-carbon electrode for measurement of O 2 •− and electrochemical characterization of O 2 •− released by cells following UVR irradiation. a Cyclic voltammetry (CV) curves measured with <t>PBS</t> and PBS plus different concentrations of KO 2 (nM). a : 0, b : 5, c : 10, d : 20, e : 40, f : 60, g : 80, h : 100, i : 150, j : 200; inset: CV curves measured with PBS (black), PBS plus KO 2 (150 nM, blue), and KO 2 solution added with SOD (300 U mL − 1 , red). b Calibration curve for serial concentrations of O 2 •− . c O 2 •− CV curves of cells under irradiation, a. UV, b . <t>UVA,</t> c . UVB, d . Sham-irradiation control. d Histogram of peak current changes compared to sham-irradiated cells (black column, n = 3) and generation of O 2 •− upon irradiation with UV as quantified by the O 2 •− assay kit: increase of absorbance at 450 nm (grey column, n = 3). SOD: superoxide dismutase. UV (10.5 J cm − 2 = 105 kJ m − 2 ), UVA (10 J cm − 2 = 100 kJ m − 2 ), UVB (0.5 J cm − 2 = 5 kJ m − 2 )

    https://www.bioz.com/result/phosphate buffer saline pbs/product/Beyotime
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphate buffer saline pbs - by Bioz Stars, 2021-04
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    Images

    1) Product Images from "Label-free electrochemical sensor to investigate the effect of tocopherol on generation of superoxide ions following UV irradiation"

    Article Title: Label-free electrochemical sensor to investigate the effect of tocopherol on generation of superoxide ions following UV irradiation

    Journal: Journal of Biological Engineering

    doi: 10.1186/s13036-018-0099-2

    Calibration of CNT@DNA-Mn 3 (PO 4 ) 2 modified glass-carbon electrode for measurement of O 2 •− and electrochemical characterization of O 2 •− released by cells following UVR irradiation. a Cyclic voltammetry (CV) curves measured with PBS and PBS plus different concentrations of KO 2 (nM). a : 0, b : 5, c : 10, d : 20, e : 40, f : 60, g : 80, h : 100, i : 150, j : 200; inset: CV curves measured with PBS (black), PBS plus KO 2 (150 nM, blue), and KO 2 solution added with SOD (300 U mL − 1 , red). b Calibration curve for serial concentrations of O 2 •− . c O 2 •− CV curves of cells under irradiation, a. UV, b . UVA, c . UVB, d . Sham-irradiation control. d Histogram of peak current changes compared to sham-irradiated cells (black column, n = 3) and generation of O 2 •− upon irradiation with UV as quantified by the O 2 •− assay kit: increase of absorbance at 450 nm (grey column, n = 3). SOD: superoxide dismutase. UV (10.5 J cm − 2 = 105 kJ m − 2 ), UVA (10 J cm − 2 = 100 kJ m − 2 ), UVB (0.5 J cm − 2 = 5 kJ m − 2 )
    Figure Legend Snippet: Calibration of CNT@DNA-Mn 3 (PO 4 ) 2 modified glass-carbon electrode for measurement of O 2 •− and electrochemical characterization of O 2 •− released by cells following UVR irradiation. a Cyclic voltammetry (CV) curves measured with PBS and PBS plus different concentrations of KO 2 (nM). a : 0, b : 5, c : 10, d : 20, e : 40, f : 60, g : 80, h : 100, i : 150, j : 200; inset: CV curves measured with PBS (black), PBS plus KO 2 (150 nM, blue), and KO 2 solution added with SOD (300 U mL − 1 , red). b Calibration curve for serial concentrations of O 2 •− . c O 2 •− CV curves of cells under irradiation, a. UV, b . UVA, c . UVB, d . Sham-irradiation control. d Histogram of peak current changes compared to sham-irradiated cells (black column, n = 3) and generation of O 2 •− upon irradiation with UV as quantified by the O 2 •− assay kit: increase of absorbance at 450 nm (grey column, n = 3). SOD: superoxide dismutase. UV (10.5 J cm − 2 = 105 kJ m − 2 ), UVA (10 J cm − 2 = 100 kJ m − 2 ), UVB (0.5 J cm − 2 = 5 kJ m − 2 )

    Techniques Used: Modification, Irradiation

    2) Product Images from "Label-free electrochemical sensor to investigate the effect of tocopherol on generation of superoxide ions following UV irradiation"

    Article Title: Label-free electrochemical sensor to investigate the effect of tocopherol on generation of superoxide ions following UV irradiation

    Journal: Journal of Biological Engineering

    doi: 10.1186/s13036-018-0099-2

    Calibration of CNT@DNA-Mn 3 (PO 4 ) 2 modified glass-carbon electrode for measurement of O 2 •− and electrochemical characterization of O 2 •− released by cells following UVR irradiation. a Cyclic voltammetry (CV) curves measured with PBS and PBS plus different concentrations of KO 2 (nM). a : 0, b : 5, c : 10, d : 20, e : 40, f : 60, g : 80, h : 100, i : 150, j : 200; inset: CV curves measured with PBS (black), PBS plus KO 2 (150 nM, blue), and KO 2 solution added with SOD (300 U mL − 1 , red). b Calibration curve for serial concentrations of O 2 •− . c O 2 •− CV curves of cells under irradiation, a. UV, b . UVA, c . UVB, d . Sham-irradiation control. d Histogram of peak current changes compared to sham-irradiated cells (black column, n = 3) and generation of O 2 •− upon irradiation with UV as quantified by the O 2 •− assay kit: increase of absorbance at 450 nm (grey column, n = 3). SOD: superoxide dismutase. UV (10.5 J cm − 2 = 105 kJ m − 2 ), UVA (10 J cm − 2 = 100 kJ m − 2 ), UVB (0.5 J cm − 2 = 5 kJ m − 2 )
    Figure Legend Snippet: Calibration of CNT@DNA-Mn 3 (PO 4 ) 2 modified glass-carbon electrode for measurement of O 2 •− and electrochemical characterization of O 2 •− released by cells following UVR irradiation. a Cyclic voltammetry (CV) curves measured with PBS and PBS plus different concentrations of KO 2 (nM). a : 0, b : 5, c : 10, d : 20, e : 40, f : 60, g : 80, h : 100, i : 150, j : 200; inset: CV curves measured with PBS (black), PBS plus KO 2 (150 nM, blue), and KO 2 solution added with SOD (300 U mL − 1 , red). b Calibration curve for serial concentrations of O 2 •− . c O 2 •− CV curves of cells under irradiation, a. UV, b . UVA, c . UVB, d . Sham-irradiation control. d Histogram of peak current changes compared to sham-irradiated cells (black column, n = 3) and generation of O 2 •− upon irradiation with UV as quantified by the O 2 •− assay kit: increase of absorbance at 450 nm (grey column, n = 3). SOD: superoxide dismutase. UV (10.5 J cm − 2 = 105 kJ m − 2 ), UVA (10 J cm − 2 = 100 kJ m − 2 ), UVB (0.5 J cm − 2 = 5 kJ m − 2 )

    Techniques Used: Modification, Irradiation

    Related Articles

    Mouse Assay:

    Article Title: Ezrin/NF-κB Pathway Regulates EGF-induced Epithelial-Mesenchymal Transition (EMT), Metastasis, and Progression of Osteosarcoma
    Article Snippet: MG63 and U20S cells with silencing Ezrin were injected subcutaneously into the armpits of the mice according to the method described in a previous report [ ]. .. When the mice developed palpable xenografts (more than 0.5 cm diameter), we injected the tumor with phosphate-buffered solution (PBS) (Beyotime) or EGF (0.02 mg/kg) twice a week for 5 weeks. .. Eight weeks later, tumor xenografts were extracted, weighed, measured, and frozen in −178°C liquid nitrogen or harvested with tissue lysate buffer (Beyotime).

    Injection:

    Article Title: Ezrin/NF-κB Pathway Regulates EGF-induced Epithelial-Mesenchymal Transition (EMT), Metastasis, and Progression of Osteosarcoma
    Article Snippet: MG63 and U20S cells with silencing Ezrin were injected subcutaneously into the armpits of the mice according to the method described in a previous report [ ]. .. When the mice developed palpable xenografts (more than 0.5 cm diameter), we injected the tumor with phosphate-buffered solution (PBS) (Beyotime) or EGF (0.02 mg/kg) twice a week for 5 weeks. .. Eight weeks later, tumor xenografts were extracted, weighed, measured, and frozen in −178°C liquid nitrogen or harvested with tissue lysate buffer (Beyotime).

    other:

    Article Title: Fugan Wan alleviates hepatic fibrosis by inhibiting ACE/Ang II/AT-1R signaling pathway and enhancing ACE2/Ang 1-7/Mas signaling pathway in hepatic fibrosis rat models
    Article Snippet: The rats were anaesthetized using 3% pentobarbital sodium (Beyotime Biotech.

    Fluorescence:

    Article Title: Evaluation of plasma cytokine protein array profile: the highlighted PDGF-BB in rheumatoid arthritis.
    Article Snippet: The cytokines play critical roles in the complex pathogenesis of rheumatoid arthritis (RA), but the specific cytokines are still in need of being discovered. .. The cytokines play critical roles in the complex pathogenesis of rheumatoid arthritis (RA), but the specific cytokines are still in need of being discovered. .. The cytokines play critical roles in the complex pathogenesis of rheumatoid arthritis (RA), but the specific cytokines are still in need of being discovered.

    FACS:

    Article Title: Evaluation of plasma cytokine protein array profile: the highlighted PDGF-BB in rheumatoid arthritis.
    Article Snippet: The cytokines play critical roles in the complex pathogenesis of rheumatoid arthritis (RA), but the specific cytokines are still in need of being discovered. .. The cytokines play critical roles in the complex pathogenesis of rheumatoid arthritis (RA), but the specific cytokines are still in need of being discovered. .. The cytokines play critical roles in the complex pathogenesis of rheumatoid arthritis (RA), but the specific cytokines are still in need of being discovered.

    Protein Concentration:

    Article Title: Zishenwan Decreases Kidney Damage in Recurrent Urinary Tract Infection through the Inhibition of Toll-Like Receptor 4 Signal
    Article Snippet: Briefly, cells were collected, washed with PBS, and resuspended in 200 μ L binding buffer containing 5 μ L annexin V (10 μ g/mL) and 10 μ L PI (20 μ g/mL) for 30 min. Then samples were immediately analyzed using flow cytometry. .. Rat bladder smooth muscle cells and homogenate treated samples of tissues were lysed in RIPA (Beyotime, Shanghai, China) and the protein concentration was determined by a Bradford kit (Beyotime, Shanghai, China). .. The proteins were treated with SDS sample buffer at 100°C for 10 min and resolved by SDS-polyacrylamide gel electrophoresis (10% acrylamide) and transferred to polyvinylidene fluoride (PVDF) membrane.

    Western Blot:

    Article Title: Homozygous mutations in DZIP1 can induce asthenoteratospermia with severe MMAF
    Article Snippet: The empty and recombinant plasmids were transfected into HEK293T cells using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s recommendations. .. Western blottingTransfected cells and half of a mouse testis were homogenised in 200 µL RIPA (Beyotime) using a pellet pestle motor homogeniser and then heated at 100°C for 15 min. Lysates were fractionated by SDS-PAGE on 10% polyacrylamide gels, transferred to polyvinylidene fluoride membranes, and the membranes were blocked in TBST (3% Bovine serum albumin in tris-buffered saline with Tween-20) for 1 hour at room temperature (RT). .. Anti-DZIP1 antibody (mouse monoclonal, Santa Cruz) was diluted 1:1000 in TBST and incubated with the membranes overnight at 4°C.

    SDS Page:

    Article Title: Homozygous mutations in DZIP1 can induce asthenoteratospermia with severe MMAF
    Article Snippet: The empty and recombinant plasmids were transfected into HEK293T cells using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s recommendations. .. Western blottingTransfected cells and half of a mouse testis were homogenised in 200 µL RIPA (Beyotime) using a pellet pestle motor homogeniser and then heated at 100°C for 15 min. Lysates were fractionated by SDS-PAGE on 10% polyacrylamide gels, transferred to polyvinylidene fluoride membranes, and the membranes were blocked in TBST (3% Bovine serum albumin in tris-buffered saline with Tween-20) for 1 hour at room temperature (RT). .. Anti-DZIP1 antibody (mouse monoclonal, Santa Cruz) was diluted 1:1000 in TBST and incubated with the membranes overnight at 4°C.

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    Beyotime phosphate buffered solution pbs
    Reducing Ezrin suppressed metastasis of <t>EGF-injected</t> MG63 and U20S xenografts of BALB/c-nu mice. ( A ) BALB/c-mu mouse with osteosarcoma xenografts. ( B ) Liver and lung weights of tumorigenic mice. ( C ) HPRT mRNA level in livers and lungs of tumorigenic mice. ( D ) E-cadherin, vimentin, and p65 protein levels in livers and lungs of tumorigenic mice. (HPRT, hypoxanthine guanine phosphoribosyl transferase; <t>Con.+PBS,</t> tumor xenografts and PBS injection; siRNA+PBS, tumor xenografts with silencing Ezrin and PBS injection; Con.+EGF, tumor xenografts and EGF injection; siRNA+EGF, tumor xenografts with silencing Ezrin and EGF injection; * compared to Con.+PBS, P
    Phosphate Buffered Solution Pbs, supplied by Beyotime, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffered solution pbs/product/Beyotime
    Average 93 stars, based on 1 article reviews
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    99
    Beyotime goat anti rabbit igg
    Capillary electrophoresis (CE) based Western blot assay conducted for detection of recombinant LamB protein in protein extracts of transgenic Wolffia . (A) Gel-like image viewed from Western blot assay. Left to right: BTL: Biotinylated ladder WT: Un-transformed wild type plant, S1–S3: Transformed plants. Anti- c- myc mouse monoclonal antibody was used as a primary antibody and the <t>HRP-conjugated</t> mouse anti-mouse <t>IgG</t> antibody used as a secondary antibody. (B) Signal intensity (area) shown in Capillary electrophoresis (CE) based Western blot assay for detection of recombinant LamB protein in protein extracts from the fronds of wild type and transformed Wolffia . WT - Wild type Wolffia , S1–S3 transgenic Wolffia lines.
    Goat Anti Rabbit Igg, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Beyotime pbs
    <t>SHARPIN</t> is mainly expressed in the cytoplasm of cells in the normal tissues recruited, with the exception for the normal lung tissue. (A) Liver, (B) larynx, (C) pancreas, (D) kidney, (E) breast, (F) lung samples. (G) Formalin fixed and paraffin embedded normal skin sample incubated with anti-SHARPIN antibody and (H) normal skin sample incubated with <t>PBS,</t> which acted as the imunofluorescence negative control. Blue and green staining in figure of immunofluorescence indicate nucleus staining and SHARPIN positive staining of cells, respectively (A-H, original magnification, ×200). SHARPIN, Shank-associated RH domain-interacting protein; PBS, phosphate-buffered saline buffer.
    Pbs, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Beyotime phosphate buffered saline pbs
    Construction and characterization of pIA. ( A ) These DNA sequences were subcloned into the Kpn I, BamH I and Xho I sites of pVAX1 to construct pVAX1-IL22 (pIL-22) and pVAX1-IA (pIA) respectively. ( B ) Expression of the IL-22 and ApoA-I fusion protein was detected by western blot from the indicated amount in HEK-293T cells after <t>transfection</t> with pIA, pVAX1 vector, or <t>PBS</t> (control group). ( C ) Densitometric values were quantified and normalized to control ( n = 3; mean ± SD; ** P
    Phosphate Buffered Saline Pbs, supplied by Beyotime, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    Image Search Results


    Reducing Ezrin suppressed metastasis of EGF-injected MG63 and U20S xenografts of BALB/c-nu mice. ( A ) BALB/c-mu mouse with osteosarcoma xenografts. ( B ) Liver and lung weights of tumorigenic mice. ( C ) HPRT mRNA level in livers and lungs of tumorigenic mice. ( D ) E-cadherin, vimentin, and p65 protein levels in livers and lungs of tumorigenic mice. (HPRT, hypoxanthine guanine phosphoribosyl transferase; Con.+PBS, tumor xenografts and PBS injection; siRNA+PBS, tumor xenografts with silencing Ezrin and PBS injection; Con.+EGF, tumor xenografts and EGF injection; siRNA+EGF, tumor xenografts with silencing Ezrin and EGF injection; * compared to Con.+PBS, P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Ezrin/NF-κB Pathway Regulates EGF-induced Epithelial-Mesenchymal Transition (EMT), Metastasis, and Progression of Osteosarcoma

    doi: 10.12659/MSM.906945

    Figure Lengend Snippet: Reducing Ezrin suppressed metastasis of EGF-injected MG63 and U20S xenografts of BALB/c-nu mice. ( A ) BALB/c-mu mouse with osteosarcoma xenografts. ( B ) Liver and lung weights of tumorigenic mice. ( C ) HPRT mRNA level in livers and lungs of tumorigenic mice. ( D ) E-cadherin, vimentin, and p65 protein levels in livers and lungs of tumorigenic mice. (HPRT, hypoxanthine guanine phosphoribosyl transferase; Con.+PBS, tumor xenografts and PBS injection; siRNA+PBS, tumor xenografts with silencing Ezrin and PBS injection; Con.+EGF, tumor xenografts and EGF injection; siRNA+EGF, tumor xenografts with silencing Ezrin and EGF injection; * compared to Con.+PBS, P

    Article Snippet: When the mice developed palpable xenografts (more than 0.5 cm diameter), we injected the tumor with phosphate-buffered solution (PBS) (Beyotime) or EGF (0.02 mg/kg) twice a week for 5 weeks.

    Techniques: Injection, Mouse Assay

    Capillary electrophoresis (CE) based Western blot assay conducted for detection of recombinant LamB protein in protein extracts of transgenic Wolffia . (A) Gel-like image viewed from Western blot assay. Left to right: BTL: Biotinylated ladder WT: Un-transformed wild type plant, S1–S3: Transformed plants. Anti- c- myc mouse monoclonal antibody was used as a primary antibody and the HRP-conjugated mouse anti-mouse IgG antibody used as a secondary antibody. (B) Signal intensity (area) shown in Capillary electrophoresis (CE) based Western blot assay for detection of recombinant LamB protein in protein extracts from the fronds of wild type and transformed Wolffia . WT - Wild type Wolffia , S1–S3 transgenic Wolffia lines.

    Journal: Frontiers in Immunology

    Article Title: Expression of LamB Vaccine Antigen in Wolffia globosa (Duck Weed) Against Fish Vibriosis

    doi: 10.3389/fimmu.2020.01857

    Figure Lengend Snippet: Capillary electrophoresis (CE) based Western blot assay conducted for detection of recombinant LamB protein in protein extracts of transgenic Wolffia . (A) Gel-like image viewed from Western blot assay. Left to right: BTL: Biotinylated ladder WT: Un-transformed wild type plant, S1–S3: Transformed plants. Anti- c- myc mouse monoclonal antibody was used as a primary antibody and the HRP-conjugated mouse anti-mouse IgG antibody used as a secondary antibody. (B) Signal intensity (area) shown in Capillary electrophoresis (CE) based Western blot assay for detection of recombinant LamB protein in protein extracts from the fronds of wild type and transformed Wolffia . WT - Wild type Wolffia , S1–S3 transgenic Wolffia lines.

    Article Snippet: HRP conjugated with goat-anti rabbit IgG (1:1000 diluted in PBS-TS) was added and incubated for another 1 h at 37°C and was then reacted with o-Phenylenediamine (OPD) substrate (Beyotime) for 10 min.

    Techniques: Electrophoresis, Western Blot, Recombinant, Transgenic Assay, Transformation Assay

    SHARPIN is mainly expressed in the cytoplasm of cells in the normal tissues recruited, with the exception for the normal lung tissue. (A) Liver, (B) larynx, (C) pancreas, (D) kidney, (E) breast, (F) lung samples. (G) Formalin fixed and paraffin embedded normal skin sample incubated with anti-SHARPIN antibody and (H) normal skin sample incubated with PBS, which acted as the imunofluorescence negative control. Blue and green staining in figure of immunofluorescence indicate nucleus staining and SHARPIN positive staining of cells, respectively (A-H, original magnification, ×200). SHARPIN, Shank-associated RH domain-interacting protein; PBS, phosphate-buffered saline buffer.

    Journal: Oncology Letters

    Article Title: Shank-associated RH domain-interacting protein expression is upregulated in entodermal and mesodermal cancer or downregulated in ectodermal malignancy

    doi: 10.3892/ol.2018.9514

    Figure Lengend Snippet: SHARPIN is mainly expressed in the cytoplasm of cells in the normal tissues recruited, with the exception for the normal lung tissue. (A) Liver, (B) larynx, (C) pancreas, (D) kidney, (E) breast, (F) lung samples. (G) Formalin fixed and paraffin embedded normal skin sample incubated with anti-SHARPIN antibody and (H) normal skin sample incubated with PBS, which acted as the imunofluorescence negative control. Blue and green staining in figure of immunofluorescence indicate nucleus staining and SHARPIN positive staining of cells, respectively (A-H, original magnification, ×200). SHARPIN, Shank-associated RH domain-interacting protein; PBS, phosphate-buffered saline buffer.

    Article Snippet: The anti-SHARPIN antibody (BD Biosciences, Franklin Lakes, NJ, USA) was diluted (1:400) with PBS (Beyotime Institute of Biotechnology) and incubated for overnight at 4°C after decanting immunology staining blocking buffer.

    Techniques: Incubation, Negative Control, Staining, Immunofluorescence

    Construction and characterization of pIA. ( A ) These DNA sequences were subcloned into the Kpn I, BamH I and Xho I sites of pVAX1 to construct pVAX1-IL22 (pIL-22) and pVAX1-IA (pIA) respectively. ( B ) Expression of the IL-22 and ApoA-I fusion protein was detected by western blot from the indicated amount in HEK-293T cells after transfection with pIA, pVAX1 vector, or PBS (control group). ( C ) Densitometric values were quantified and normalized to control ( n = 3; mean ± SD; ** P

    Journal: Theranostics

    Article Title: Tethering Interleukin-22 to Apolipoprotein A-I Ameliorates Mice from Acetaminophen-induced Liver Injury

    doi: 10.7150/thno.20955

    Figure Lengend Snippet: Construction and characterization of pIA. ( A ) These DNA sequences were subcloned into the Kpn I, BamH I and Xho I sites of pVAX1 to construct pVAX1-IL22 (pIL-22) and pVAX1-IA (pIA) respectively. ( B ) Expression of the IL-22 and ApoA-I fusion protein was detected by western blot from the indicated amount in HEK-293T cells after transfection with pIA, pVAX1 vector, or PBS (control group). ( C ) Densitometric values were quantified and normalized to control ( n = 3; mean ± SD; ** P

    Article Snippet: The cells were harvested 1 day after transfection, washed three times with phosphate buffered saline (PBS), and lysed with cell lysis buffer (Beyotime Biotechnology, China).

    Techniques: Construct, IA, Expressing, Western Blot, Transfection, Plasmid Preparation