phosphatase inhibitors  (Thermo Fisher)


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    Name:
    Halt Phosphatase Inhibitor Cocktail
    Description:
    Thermo Scientific Halt Phosphatase Inhibitor Cocktail preserves the phosphorylation state of proteins during and after cell lysis or tissue protein extraction Features of Halt Phosphatase Inhibitor Cocktail • Devoid of protease inhibitors To protect against both proteases and phosphatases use Halt Protease and Phosphatase Inhibitor Cocktail • Dialyzable Either dialyze or desalt sample to effectively remove inhibitors from cell extracts before performing procedures that are discovered to be incompatible with cocktail components • Supplied as a 100X concentrate effective when used at a final concentration of 1X however samples that contain particularly high levels of phosphatase activity may require a final concentration of 2 3X • Compatibility compatible with most detergent based cell lysis reagents including all Thermo Scientific Pierce Cell Lysis Solutions • All in one format inhibits both serine threonine phosphatases and protein tyrosine phosphatases • Compatiblewith popular protein assays works with standard protein assays including BCA and coomassie Bradford assays • Convenient storage refrigerator 2 to 8°C storage saves valuable space in the freezer and provides for immediate use • Multiple sizes select a package size of the 100X cocktail that is ideal for any particular scale and workflow of protein extraction experiment Halt Phosphatase Inhibitor Cocktail protects phosphoproteins from serine threonine phosphatases as well as protein tyrosine phosphatases PTPs during cell lysis The cocktail contains a mixture of four inhibitors of broad specificity including sodium fluoride sodium orthovanadate sodium pyrophosphate and beta glycerophosphate Conveniently packaged in a 100X format the cocktail is stable at 4°C and is suitable for use with any cell or tissue extract The cocktail preserves protein phosphorylation in immunoprecipitation and kinase assays Phosphorylation and dephosphorylation comprise a molecular on off switch that regulates many key biological pathways within the cell including signal transduction cell division and apoptosis To effectively study phosphorylation activation states one must inactivate phosphatases to prevent their uncontrolled activity upon cell lysis Halt Phosphatase Inhibitor Cocktail accomplishes this task Related Products Pierce Phosphatase Inhibitor Mini Tablets
    Catalog Number:
    78420
    Price:
    None
    Applications:
    Cell Lysis & Fractionation|Protease and Phosphatase Inhibition|Protein Biology|Protein Purification & Isolation
    Category:
    Lab Reagents and Chemicals
    Buy from Supplier


    Structured Review

    Thermo Fisher phosphatase inhibitors
    Thermo Scientific Halt Phosphatase Inhibitor Cocktail preserves the phosphorylation state of proteins during and after cell lysis or tissue protein extraction Features of Halt Phosphatase Inhibitor Cocktail • Devoid of protease inhibitors To protect against both proteases and phosphatases use Halt Protease and Phosphatase Inhibitor Cocktail • Dialyzable Either dialyze or desalt sample to effectively remove inhibitors from cell extracts before performing procedures that are discovered to be incompatible with cocktail components • Supplied as a 100X concentrate effective when used at a final concentration of 1X however samples that contain particularly high levels of phosphatase activity may require a final concentration of 2 3X • Compatibility compatible with most detergent based cell lysis reagents including all Thermo Scientific Pierce Cell Lysis Solutions • All in one format inhibits both serine threonine phosphatases and protein tyrosine phosphatases • Compatiblewith popular protein assays works with standard protein assays including BCA and coomassie Bradford assays • Convenient storage refrigerator 2 to 8°C storage saves valuable space in the freezer and provides for immediate use • Multiple sizes select a package size of the 100X cocktail that is ideal for any particular scale and workflow of protein extraction experiment Halt Phosphatase Inhibitor Cocktail protects phosphoproteins from serine threonine phosphatases as well as protein tyrosine phosphatases PTPs during cell lysis The cocktail contains a mixture of four inhibitors of broad specificity including sodium fluoride sodium orthovanadate sodium pyrophosphate and beta glycerophosphate Conveniently packaged in a 100X format the cocktail is stable at 4°C and is suitable for use with any cell or tissue extract The cocktail preserves protein phosphorylation in immunoprecipitation and kinase assays Phosphorylation and dephosphorylation comprise a molecular on off switch that regulates many key biological pathways within the cell including signal transduction cell division and apoptosis To effectively study phosphorylation activation states one must inactivate phosphatases to prevent their uncontrolled activity upon cell lysis Halt Phosphatase Inhibitor Cocktail accomplishes this task Related Products Pierce Phosphatase Inhibitor Mini Tablets
    https://www.bioz.com/result/phosphatase inhibitors/product/Thermo Fisher
    Average 99 stars, based on 1380 article reviews
    Price from $9.99 to $1999.99
    phosphatase inhibitors - by Bioz Stars, 2020-09
    99/100 stars

    Images

    Related Articles

    Chromatography:

    Article Title: Bacterial Carriers for Glioblastoma Therapy
    Article Snippet: .. Tissue Homogenization prior to Liquid Chromatography-Tandem Mass Spectrometry at Emory Proteomics Core Lab Brain tissue was individually homogenized in 300 μL of urea lysis buffer (8 M urea, 100 mM NaHPO4 , pH 8.5) which had 3 μL (100× stock) of HALT protease and phosphatase inhibitor cocktail (Pierce). .. Homogenization was performed using a Bullet Blender (Next Advance) according to manufacturer protocols.

    Homogenization:

    Article Title: Bacterial Carriers for Glioblastoma Therapy
    Article Snippet: .. Tissue Homogenization prior to Liquid Chromatography-Tandem Mass Spectrometry at Emory Proteomics Core Lab Brain tissue was individually homogenized in 300 μL of urea lysis buffer (8 M urea, 100 mM NaHPO4 , pH 8.5) which had 3 μL (100× stock) of HALT protease and phosphatase inhibitor cocktail (Pierce). .. Homogenization was performed using a Bullet Blender (Next Advance) according to manufacturer protocols.

    Protease Inhibitor:

    Article Title: Functional modulation of insulin-like growth factor binding protein-3 expression in melanoma
    Article Snippet: .. Cell lysates were prepared in PBS containing 1x Halt protease inhibitor cocktail and 1x Halt phosphatase inhibitor cocktail (Pierce, Rockford, IL) centrifuged at 3500 r.p.m. for 10 min at 4°C. .. Proteins (10–15 ug) from each sample were subjected to SDS/polyacrylamide gel electrophoresis (PAGE) and transferred onto a nitrocellulose membrane.

    Article Title: Antigen Presentation Profiling Reveals Recognition of Lymphoma Immunoglobulin Neoantigens
    Article Snippet: .. In brief, cells were lysed in 8M urea, 150 mM NaCl, 5 mM DTT, 50 mM Tris pH 8 supplemented with Complete Protease Inhibitor Cocktail tablet (Roche) and 1x Halt™ Protease and Phosphatase Inhibitor Cocktail (ThermoFisher Scientific). .. Following lysis, the lysate was centrifuged at 13,200 rpm for 15 min, the supernatant was transferred to fresh tubes and a second round of centrifugation was performed.

    Article Title: Aspergillus fumigatus responds to natural killer (NK) cells with upregulation of stress related genes and inhibits the immunoregulatory function of NK cells
    Article Snippet: .. Cells were lysed with RIPA Lysis and Extraction Buffer Kit (Thermo Scientific) containing HALT Phosphatase and Protease Inhibitor Cocktails (both Thermo Scientific). .. Proteins were separated by SDS-PAGE using 4-15% Mini Protean TGX Precast Protein Gels (Bio-Rad).

    Incubation:

    Article Title: MG132‐induced progerin clearance is mediated by autophagy activation and splicing regulation
    Article Snippet: .. The supernatant was removed, and Dynabeads were incubated with 200 μl cell lysate (200 μg protein) extracted using NP40 Cell Lysis Buffer (Invitrogen) containing protease, Phosphatase Inhibitor Cocktail (Thermo Scientific), and 5 mM NEM, and the tubes were rotated at room temperature for 30 min. ..

    Mass Spectrometry:

    Article Title: Bacterial Carriers for Glioblastoma Therapy
    Article Snippet: .. Tissue Homogenization prior to Liquid Chromatography-Tandem Mass Spectrometry at Emory Proteomics Core Lab Brain tissue was individually homogenized in 300 μL of urea lysis buffer (8 M urea, 100 mM NaHPO4 , pH 8.5) which had 3 μL (100× stock) of HALT protease and phosphatase inhibitor cocktail (Pierce). .. Homogenization was performed using a Bullet Blender (Next Advance) according to manufacturer protocols.

    Western Blot:

    Article Title: LY3009120, a panRAF inhibitor, has significant anti-tumor activity in BRAF and KRAS mutant preclinical models of colorectal cancer
    Article Snippet: .. Tumor xenograft preparation for western blotting Tumors harvested upon study completion were briefly thawed and 20-30 mg of tissue sample was placed in a tube containing Lysing Matrix A (MP Biomedicals) and 650 μL of lysis buffer (XY buffer: 1% Triton X100, 25 mM Tris pH7.5, 150mM NaCl, 1mM EDTA/1mM EGTA) supplemented with 3X Halt protease/phosphatase inhibitors (Thermo Scientific). .. Samples were homogenized for 20 seconds in a FastPrep FP120 Cell Disrupter (Thermo Electron) and allowed to sit on ice for 1 hr.

    Lysis:

    Article Title: LY3009120, a panRAF inhibitor, has significant anti-tumor activity in BRAF and KRAS mutant preclinical models of colorectal cancer
    Article Snippet: .. Tumor xenograft preparation for western blotting Tumors harvested upon study completion were briefly thawed and 20-30 mg of tissue sample was placed in a tube containing Lysing Matrix A (MP Biomedicals) and 650 μL of lysis buffer (XY buffer: 1% Triton X100, 25 mM Tris pH7.5, 150mM NaCl, 1mM EDTA/1mM EGTA) supplemented with 3X Halt protease/phosphatase inhibitors (Thermo Scientific). .. Samples were homogenized for 20 seconds in a FastPrep FP120 Cell Disrupter (Thermo Electron) and allowed to sit on ice for 1 hr.

    Article Title: MG132‐induced progerin clearance is mediated by autophagy activation and splicing regulation
    Article Snippet: .. The supernatant was removed, and Dynabeads were incubated with 200 μl cell lysate (200 μg protein) extracted using NP40 Cell Lysis Buffer (Invitrogen) containing protease, Phosphatase Inhibitor Cocktail (Thermo Scientific), and 5 mM NEM, and the tubes were rotated at room temperature for 30 min. ..

    Article Title: Bacterial Carriers for Glioblastoma Therapy
    Article Snippet: .. Tissue Homogenization prior to Liquid Chromatography-Tandem Mass Spectrometry at Emory Proteomics Core Lab Brain tissue was individually homogenized in 300 μL of urea lysis buffer (8 M urea, 100 mM NaHPO4 , pH 8.5) which had 3 μL (100× stock) of HALT protease and phosphatase inhibitor cocktail (Pierce). .. Homogenization was performed using a Bullet Blender (Next Advance) according to manufacturer protocols.

    Article Title: Aspergillus fumigatus responds to natural killer (NK) cells with upregulation of stress related genes and inhibits the immunoregulatory function of NK cells
    Article Snippet: .. Cells were lysed with RIPA Lysis and Extraction Buffer Kit (Thermo Scientific) containing HALT Phosphatase and Protease Inhibitor Cocktails (both Thermo Scientific). .. Proteins were separated by SDS-PAGE using 4-15% Mini Protean TGX Precast Protein Gels (Bio-Rad).

    Article Title: AMP-activated Protein Kinase α2 Protects against Liver Injury from Metastasized Tumors via Reduced Glucose Deprivation-induced Oxidative Stress *
    Article Snippet: .. Samples of liver tissues or cells were homogenized in lysis buffer (Thermo Scientific) with the HALT protease and phosphatase inhibitor cocktails (Thermo Scientific), and then the homogenates were centrifuged at 12,000 × g for 30 min at 4 °C. .. After protein concentration was determined in supernatants by using the Bio-Rad protein assay based on the method of Bradford, protein samples were loaded on 10% SDS-PAGE and then transferred to a nitrocellulose membrane.

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  • 87
    Thermo Fisher gene exp ppp1r1b hs00259967 m1
    Neuroimaging analyses (structure and function) of common haplotype in <t>PPP1R1B</t> in control sample of white subjects. Top row shows haplotype effects on volume ( A ) or activation ( C and E ) in striatum; bottom row shows haplotype effects on structural ( B ) and functional ( D and F ) connectivity of striatum with prefrontal cortex. Structural MRI analyses (voxel-based morphometry): ( A ) significantly reduced volume in striatum ( P
    Gene Exp Ppp1r1b Hs00259967 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp ppp1r1b hs00259967 m1/product/Thermo Fisher
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp ppp1r1b hs00259967 m1 - by Bioz Stars, 2020-09
    87/100 stars
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    95
    Thermo Fisher aebsf protease inhibitor
    Trypsin digestion of Vip3Aa using inhibitors to stop the reaction. The reactions were stopped with the addition of irreversible trypsin protease inhibitors or urea. Loading buffer was added either immediately ( a ) or 10 min after addition of the inhibitors or the urea ( b ) and the samples heated and subjected to SDS-PAGE. Lanes 1: molecular weight markers; lanes 2: untreated protoxin; lanes 3: control with no inhibitors; lanes 4: 1 mM PMSF; lanes 5: 0.1 mM <t>TLCK;</t> lanes 6: 1 mM <t>AEBSF;</t> lanes 7: 10 mM E64; lanes 8: 8 M urea. Molecular weight markers are indicated in kDa.
    Aebsf Protease Inhibitor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aebsf protease inhibitor/product/Thermo Fisher
    Average 95 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    aebsf protease inhibitor - by Bioz Stars, 2020-09
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    94
    Thermo Fisher aprotinin
    Protease inhibitor precipitation zone. The <t>aprotinin</t> protein protection zones were visibly distinct under conditions of increased amounts of trypsin on the different protein containing plates. (A) Aprotinin (2 μl of 0.3 mM in sterile dH 2 0) with
    Aprotinin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aprotinin/product/Thermo Fisher
    Average 94 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    aprotinin - by Bioz Stars, 2020-09
    94/100 stars
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    Image Search Results


    Neuroimaging analyses (structure and function) of common haplotype in PPP1R1B in control sample of white subjects. Top row shows haplotype effects on volume ( A ) or activation ( C and E ) in striatum; bottom row shows haplotype effects on structural ( B ) and functional ( D and F ) connectivity of striatum with prefrontal cortex. Structural MRI analyses (voxel-based morphometry): ( A ) significantly reduced volume in striatum ( P

    Journal: Journal of Clinical Investigation

    Article Title: Genetic evidence implicating DARPP-32 in human frontostriatal structure, function, and cognition

    doi: 10.1172/JCI30413

    Figure Lengend Snippet: Neuroimaging analyses (structure and function) of common haplotype in PPP1R1B in control sample of white subjects. Top row shows haplotype effects on volume ( A ) or activation ( C and E ) in striatum; bottom row shows haplotype effects on structural ( B ) and functional ( D and F ) connectivity of striatum with prefrontal cortex. Structural MRI analyses (voxel-based morphometry): ( A ) significantly reduced volume in striatum ( P

    Article Snippet: Three probes were based on the common exon structure as found in full-length cDNA: exons 4 and 5 (type 9967; Hs00259967), 1 and 2 (type 10; Hs00938410_m1) and 5 and 6 (type 14; Hs00938414_m1); splice variant 5183, exons 2 and 5; and t-DARPP (type 16; Hs00938416_g1).

    Techniques: Activation Assay, Functional Assay, Magnetic Resonance Imaging

    Genetic variation in PPP1R1B.

    Journal: Journal of Clinical Investigation

    Article Title: Genetic evidence implicating DARPP-32 in human frontostriatal structure, function, and cognition

    doi: 10.1172/JCI30413

    Figure Lengend Snippet: Genetic variation in PPP1R1B.

    Article Snippet: Three probes were based on the common exon structure as found in full-length cDNA: exons 4 and 5 (type 9967; Hs00259967), 1 and 2 (type 10; Hs00938410_m1) and 5 and 6 (type 14; Hs00938414_m1); splice variant 5183, exons 2 and 5; and t-DARPP (type 16; Hs00938416_g1).

    Techniques:

    Effect of PPP1R1B haplotype on mRNA expression in postmortem human brain. ( A ) Exon position of primers used for quantitative PCR for isoform amplification, showing which isoform was amplified by which probe. ( B ) Effect of PPP1R1B haplotypes on mRNA expression for the common isoform. The PPP1R1B haplotype has an impact on expression of all probes assaying the expression of the full-length mRNA (type 14, P

    Journal: Journal of Clinical Investigation

    Article Title: Genetic evidence implicating DARPP-32 in human frontostriatal structure, function, and cognition

    doi: 10.1172/JCI30413

    Figure Lengend Snippet: Effect of PPP1R1B haplotype on mRNA expression in postmortem human brain. ( A ) Exon position of primers used for quantitative PCR for isoform amplification, showing which isoform was amplified by which probe. ( B ) Effect of PPP1R1B haplotypes on mRNA expression for the common isoform. The PPP1R1B haplotype has an impact on expression of all probes assaying the expression of the full-length mRNA (type 14, P

    Article Snippet: Three probes were based on the common exon structure as found in full-length cDNA: exons 4 and 5 (type 9967; Hs00259967), 1 and 2 (type 10; Hs00938410_m1) and 5 and 6 (type 14; Hs00938414_m1); splice variant 5183, exons 2 and 5; and t-DARPP (type 16; Hs00938416_g1).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Amplification

    Genetic variation in PPP1R1B.

    Journal: Journal of Clinical Investigation

    Article Title: Genetic evidence implicating DARPP-32 in human frontostriatal structure, function, and cognition

    doi: 10.1172/JCI30413

    Figure Lengend Snippet: Genetic variation in PPP1R1B.

    Article Snippet: Three probes were based on the common exon structure as found in full-length cDNA: exons 4 and 5 (type 9967; Hs00259967), 1 and 2 (type 10; Hs00938410_m1) and 5 and 6 (type 14; Hs00938414_m1); splice variant 5183, exons 2 and 5; and t-DARPP (type 16; Hs00938416_g1).

    Techniques:

    Trypsin digestion of Vip3Aa using inhibitors to stop the reaction. The reactions were stopped with the addition of irreversible trypsin protease inhibitors or urea. Loading buffer was added either immediately ( a ) or 10 min after addition of the inhibitors or the urea ( b ) and the samples heated and subjected to SDS-PAGE. Lanes 1: molecular weight markers; lanes 2: untreated protoxin; lanes 3: control with no inhibitors; lanes 4: 1 mM PMSF; lanes 5: 0.1 mM TLCK; lanes 6: 1 mM AEBSF; lanes 7: 10 mM E64; lanes 8: 8 M urea. Molecular weight markers are indicated in kDa.

    Journal: Toxins

    Article Title: Insights into the Structure of the Vip3Aa Insecticidal Protein by Protease Digestion Analysis

    doi: 10.3390/toxins9040131

    Figure Lengend Snippet: Trypsin digestion of Vip3Aa using inhibitors to stop the reaction. The reactions were stopped with the addition of irreversible trypsin protease inhibitors or urea. Loading buffer was added either immediately ( a ) or 10 min after addition of the inhibitors or the urea ( b ) and the samples heated and subjected to SDS-PAGE. Lanes 1: molecular weight markers; lanes 2: untreated protoxin; lanes 3: control with no inhibitors; lanes 4: 1 mM PMSF; lanes 5: 0.1 mM TLCK; lanes 6: 1 mM AEBSF; lanes 7: 10 mM E64; lanes 8: 8 M urea. Molecular weight markers are indicated in kDa.

    Article Snippet: The irreversible inhibitors used to stop the trypsin action, were: PMSF (phenylmethanesulfonyl fluoride, from SIGMA), TLCK (Nα-tosyl-l -lysine chloromethyl ketone hydrochloride, from SIGMA), AEBSF protease inhibitor (from ThermoFisher, Waltham, MA, USA), and E64 (trans-epoxysuccinyl-l -leucylamido (4-guanidino) butane, from SIGMA).

    Techniques: SDS Page, Molecular Weight

    Protease inhibitor precipitation zone. The aprotinin protein protection zones were visibly distinct under conditions of increased amounts of trypsin on the different protein containing plates. (A) Aprotinin (2 μl of 0.3 mM in sterile dH 2 0) with

    Journal: Journal of microbiological methods

    Article Title: A Culture-Based Method for Determining the Production of Secreted Protease Inhibitors

    doi: 10.1016/j.mimet.2014.02.019

    Figure Lengend Snippet: Protease inhibitor precipitation zone. The aprotinin protein protection zones were visibly distinct under conditions of increased amounts of trypsin on the different protein containing plates. (A) Aprotinin (2 μl of 0.3 mM in sterile dH 2 0) with

    Article Snippet: Protease inhibitors were α-2-macroglobulin (7.8 mg/ml in sterile water; Thermo Fisher), aprotinin (0.3 mM in sterile dH2 0; Thermo Fisher), leupeptin (10 mM in sterile dH2 0; Thermo Fisher), and bestatin (1 mM in methanol; Thermo Fisher) that were pipeted as 2.0 μl drops onto the surface of the protein containing plates and allowed to be absorbed into the plate and diffuse for 1 hour.

    Techniques: Protease Inhibitor