phorbol 12 myristate 13 acetate pma  (Valiant)

 
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  • 92
    Name:
    4α Phorbol 12 myristate 13 acetate
    Description:
    4Α Phorbol 12 Myristate 13 Acetate
    Catalog Number:
    0215898205
    Price:
    950.25
    Category:
    Life Sciences Biochemicals
    Size:
    5 mg
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    Structured Review

    Valiant phorbol 12 myristate 13 acetate pma
    4α Phorbol 12 myristate 13 acetate
    4Α Phorbol 12 Myristate 13 Acetate
    https://www.bioz.com/result/phorbol 12 myristate 13 acetate pma/product/Valiant
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phorbol 12 myristate 13 acetate pma - by Bioz Stars, 2021-05
    92/100 stars

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    Incubation:

    Article Title: Mast Cell Cytonemes as a Defense Mechanism against Coxiella burnetii
    Article Snippet: .. MCs and neutrophils (1 × 106 cells/well) were incubated in 24-well plates pretreated with fibronectin (1 mg/well; Life Technologies) for 3 h. Adherent cells were stimulated with 100 µg/ml LPS, 25 nM phorbol-12-myristate-13-acetate (PMA) (MP Biomedicals), or bacteria (bacterium-to-cell ratio of 25 and 50 bacteria per cell for S. aureus and C. burnetii , respectively) at 37°C. .. The roles of CD36 and TLR4 were studied using MCs pretreated with 5 µg/ml CD36-blocking antibodies (Abs) (mouse IgG2a; Thermo Fisher Scientific) or 10 µg/ml of the TLR4 inhibitor polymyxin B sulfate (Sigma-Aldrich) for 10 min.

    Binding Assay:

    Article Title: Steroidal Alkaloids as an Emerging Therapeutic Alternative for Investigation of Their Immunosuppressive and Hepatoprotective Potential
    Article Snippet: Hitachi UV-3200 spectrophotometer, IR Jasco A-302 model spectrophotometer, Mass spectrometer, model Jeol HX-110, BrukerAvanceAM-400 and AC-300 NMR spectrometer were used for the current study. .. The material and instrument used for assay were lithium-heparin blood collection tube with an internal vacuum for suck the blood (BD Biosciences), serum of fetal bovine from Thermo Scientific Hyclone, Rosewell Park Memorial Institute-1640 (RPMI-1640) Medium from Mediatech, Inc. (USA), Phytohemagglutin (PHA) from Sigma-Aldrich (USA), antibiotic penicillin/streptomycin and separation medium for lymphocytes (LSM) obtained from Invitrogen (USA), 3H-thymidine obtained from Amersham (UK), filters made of glass fiber obtained from Conncetorate AG (Switzerland), trypan blue from Amresco (USA), phorbol-12-myristate-13-acetate (PMA) from MP Biomedicals (France), Streptavidin-HRP, clear high -binding, enzyme-linked immunosorbent assay (ELISA) kit and plate sealer, microplates made from polystyrene, reagent pack as a substrate containing H2 O2 and TMB (Tetramethylbenzidine) from R & D system, Inc. (USA). .. Carbon tetrachloride (CCl4 ), and gelatin, olive oil from Sigma Chemical, Co. (USA), dimethyl sulfoxide (DMSO) from ThermoFisher Scientific (Loughborough, UK), for cell viability [3-(4,5-dimethylthiazole-2-yl)-2-5-diphenyltetrazolium bromide] (MTT) used obtained from ThermoFisher Scientific (Loughborough, UK).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Steroidal Alkaloids as an Emerging Therapeutic Alternative for Investigation of Their Immunosuppressive and Hepatoprotective Potential
    Article Snippet: Hitachi UV-3200 spectrophotometer, IR Jasco A-302 model spectrophotometer, Mass spectrometer, model Jeol HX-110, BrukerAvanceAM-400 and AC-300 NMR spectrometer were used for the current study. .. The material and instrument used for assay were lithium-heparin blood collection tube with an internal vacuum for suck the blood (BD Biosciences), serum of fetal bovine from Thermo Scientific Hyclone, Rosewell Park Memorial Institute-1640 (RPMI-1640) Medium from Mediatech, Inc. (USA), Phytohemagglutin (PHA) from Sigma-Aldrich (USA), antibiotic penicillin/streptomycin and separation medium for lymphocytes (LSM) obtained from Invitrogen (USA), 3H-thymidine obtained from Amersham (UK), filters made of glass fiber obtained from Conncetorate AG (Switzerland), trypan blue from Amresco (USA), phorbol-12-myristate-13-acetate (PMA) from MP Biomedicals (France), Streptavidin-HRP, clear high -binding, enzyme-linked immunosorbent assay (ELISA) kit and plate sealer, microplates made from polystyrene, reagent pack as a substrate containing H2 O2 and TMB (Tetramethylbenzidine) from R & D system, Inc. (USA). .. Carbon tetrachloride (CCl4 ), and gelatin, olive oil from Sigma Chemical, Co. (USA), dimethyl sulfoxide (DMSO) from ThermoFisher Scientific (Loughborough, UK), for cell viability [3-(4,5-dimethylthiazole-2-yl)-2-5-diphenyltetrazolium bromide] (MTT) used obtained from ThermoFisher Scientific (Loughborough, UK).

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  • 92
    Valiant phorbol 12 myristate 13 acetate
    Effects of S. thermophilus ST28 on percentages of Th17 (a) and inflammatory DC (b) in LPLs from DSS-induced colitis mice. (a) After the experiments described in Figure 3 , LPLs from DSS-induced mice orally administered ST28 or ATCC 19258 were incubated with <t>phorbol-12-myristate-13-acetate,</t> ionomycin, and brefeldin A and applied to flow cytometry to measure intracellular IL-17. (b) Separately, flow cytometric analysis of surface marker CD86 was performed using freshly prepared LPLs. LPLs from untreated DSS-induced colitis mice (−) and healthy control mice (Cont) were also assayed. ND, not detected.
    Phorbol 12 Myristate 13 Acetate, supplied by Valiant, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phorbol 12 myristate 13 acetate/product/Valiant
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phorbol 12 myristate 13 acetate - by Bioz Stars, 2021-05
    92/100 stars
      Buy from Supplier

    88
    Valiant pma treated
    Effects of S. thermophilus ST28 on percentages of Th17 (a) and inflammatory DC (b) in LPLs from DSS-induced colitis mice. (a) After the experiments described in Figure 3 , LPLs from DSS-induced mice orally administered ST28 or ATCC 19258 were incubated with <t>phorbol-12-myristate-13-acetate,</t> ionomycin, and brefeldin A and applied to flow cytometry to measure intracellular IL-17. (b) Separately, flow cytometric analysis of surface marker CD86 was performed using freshly prepared LPLs. LPLs from untreated DSS-induced colitis mice (−) and healthy control mice (Cont) were also assayed. ND, not detected.
    Pma Treated, supplied by Valiant, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pma treated/product/Valiant
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pma treated - by Bioz Stars, 2021-05
    88/100 stars
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    Image Search Results


    Effects of S. thermophilus ST28 on percentages of Th17 (a) and inflammatory DC (b) in LPLs from DSS-induced colitis mice. (a) After the experiments described in Figure 3 , LPLs from DSS-induced mice orally administered ST28 or ATCC 19258 were incubated with phorbol-12-myristate-13-acetate, ionomycin, and brefeldin A and applied to flow cytometry to measure intracellular IL-17. (b) Separately, flow cytometric analysis of surface marker CD86 was performed using freshly prepared LPLs. LPLs from untreated DSS-induced colitis mice (−) and healthy control mice (Cont) were also assayed. ND, not detected.

    Journal: Journal of Biomedicine and Biotechnology

    Article Title: Streptococcus thermophilus ST28 Ameliorates Colitis in Mice Partially by Suppression of Inflammatory Th17 Cells

    doi: 10.1155/2011/378417

    Figure Lengend Snippet: Effects of S. thermophilus ST28 on percentages of Th17 (a) and inflammatory DC (b) in LPLs from DSS-induced colitis mice. (a) After the experiments described in Figure 3 , LPLs from DSS-induced mice orally administered ST28 or ATCC 19258 were incubated with phorbol-12-myristate-13-acetate, ionomycin, and brefeldin A and applied to flow cytometry to measure intracellular IL-17. (b) Separately, flow cytometric analysis of surface marker CD86 was performed using freshly prepared LPLs. LPLs from untreated DSS-induced colitis mice (−) and healthy control mice (Cont) were also assayed. ND, not detected.

    Article Snippet: Flow Cytometric Analysis of Splenocytes The splenocytes (1 × 106 cells/mL) were incubated at 37°C for 4 hours in RPMI 1640 medium containing 0.5 μ g/mL phorbol-12-myristate-13-acetate (MP Biomedicals, Aurora, Ohio, USA), 1 μ g/mL ionomycin (Wako Pure Chemical Industries, Osaka, Japan), and 3 μ g/mL brefeldin-A (Wako Pure Chemical Industries).

    Techniques: Mouse Assay, Incubation, Flow Cytometry, Cytometry, Marker