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GE Healthcare phi29 dna polymerase
Phi29 Dna Polymerase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phi29 dna polymerase/product/GE Healthcare
Average 99 stars, based on 4 article reviews
Price from $9.99 to $1999.99
phi29 dna polymerase - by Bioz Stars, 2020-09
99/100 stars

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Polymerase Chain Reaction:

Article Title: Enhanced NADH Metabolism Involves Colistin-Induced Killing of Bacillus subtilis and Paenibacillus polymyxa
Article Snippet: .. PCR amplification reactions were performed in a final volume of 50 µL containing 20 ng genomic DNA, 100 nM each of primers, 62.5 µM each of dNTPs, 50 mM KCl, 10 mM Tris-HCl, 1.5 mM MgCl2 and 1 U Taq polymerase (Amersham Biosciences, Piscataway, BJ, USA). .. PCR amplification consisted of denaturation at 95 °C for 5 min, followed by 35 cycles of 30 s at 95 °C, 30 s at 55 °C, 2 min at 72 °C, and a final extension step at 72 °C for 10 min. At the end of the reaction, the reaction mixture was cooled to 4 °C to await further manipulations.

Article Title: A Recombination Hotspot in a Schizophrenia-Associated Region of GABRB2
Article Snippet: .. Amplification of a 760-bp DNA fragment that included SNPs S1 to S5 was performed in a 20 µl PCR mixture containing 100 ng sperm or genomic DNA, 75 nM of each of primers B2I8-214F and B2I8-214R , 50 nM of each dNTP, 2.5 mM MgCl2 and 1 U Taq DNA polymerase (Amersham). .. Stringent PCR conditions using increased annealing temperatures and a minimum number of reaction cycles were employed to prevent PCR jumping.

Article Title: dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase
Article Snippet: .. For this purpose, Taq polymerase-dependent PCR amplification in the presence of minor amounts (5%) of dUTPs labelled with differently charged Cy3 and Cy5 analogues was investigated. dUTPs labelled with electroneutral Cy3 and Cy5 analogues were used by Taq polymerase approximately one order of magnitude more effectively than dUTPs labelled with negatively charged Cy3 or Cy5 fluorophore analogues, including Amersham Cy3-dUTP and Cy5-dUTP, each of which carries negatively charged fluorophores. ..

Activity Assay:

Article Title: dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase
Article Snippet: .. This activity was not observed for Taq polymerase when dUTPs labelled with negatively charged Cy3 and Cy5 dye analogues, dU(Cy3a−)TP, dU(Cy5a−)TP and Amersham Cy5-dUTP, were used (Figures and and ). .. Nucleotidyl transferase activity of Taq polymerase in the presence of fluorescently labelled dUTPs: mass spectral analysis of primer extension products Figure illustrates the results of a mass spectral analysis of P1-(Cy3a−) primer extension products obtained using the M5 template and natural dNTPs or dNTPs in which dTTP was completely replaced by dU(Cy5a1±)TP (mass spectra 1 and 2, respectively).

Article Title: Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome.
Article Snippet: .. We have synthesized a 582,970-base pair Mycoplasma genitalium genome. .. We have synthesized a 582,970-base pair Mycoplasma genitalium genome.

Article Title: Acyclic and dideoxy terminator preferences denote divergent sugar recognition by archaeon and Taq DNA polymerases
Article Snippet: .. Taq DNA polymerase (Amersham Pharmacia Biotech, Piscataway, NJ) and its F667Y derivative Thermo Sequenase (Amersham Pharmacia Biotech) naturally lack this activity, while the archaeon DNA polymerases have been genetically modified within the conserved exonuclease motif DIE to AIA to eliminate this activity ( – ). .. Vent and Deep Vent DNA polymerases were from New England Biolabs and Pfu DNA polymerase was from Stratagene (La Jolla, CA).

Amplification:

Article Title: Enhanced NADH Metabolism Involves Colistin-Induced Killing of Bacillus subtilis and Paenibacillus polymyxa
Article Snippet: .. PCR amplification reactions were performed in a final volume of 50 µL containing 20 ng genomic DNA, 100 nM each of primers, 62.5 µM each of dNTPs, 50 mM KCl, 10 mM Tris-HCl, 1.5 mM MgCl2 and 1 U Taq polymerase (Amersham Biosciences, Piscataway, BJ, USA). .. PCR amplification consisted of denaturation at 95 °C for 5 min, followed by 35 cycles of 30 s at 95 °C, 30 s at 55 °C, 2 min at 72 °C, and a final extension step at 72 °C for 10 min. At the end of the reaction, the reaction mixture was cooled to 4 °C to await further manipulations.

Article Title: A Recombination Hotspot in a Schizophrenia-Associated Region of GABRB2
Article Snippet: .. Amplification of a 760-bp DNA fragment that included SNPs S1 to S5 was performed in a 20 µl PCR mixture containing 100 ng sperm or genomic DNA, 75 nM of each of primers B2I8-214F and B2I8-214R , 50 nM of each dNTP, 2.5 mM MgCl2 and 1 U Taq DNA polymerase (Amersham). .. Stringent PCR conditions using increased annealing temperatures and a minimum number of reaction cycles were employed to prevent PCR jumping.

Article Title: dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase
Article Snippet: .. For this purpose, Taq polymerase-dependent PCR amplification in the presence of minor amounts (5%) of dUTPs labelled with differently charged Cy3 and Cy5 analogues was investigated. dUTPs labelled with electroneutral Cy3 and Cy5 analogues were used by Taq polymerase approximately one order of magnitude more effectively than dUTPs labelled with negatively charged Cy3 or Cy5 fluorophore analogues, including Amersham Cy3-dUTP and Cy5-dUTP, each of which carries negatively charged fluorophores. ..

Genetically Modified:

Article Title: Acyclic and dideoxy terminator preferences denote divergent sugar recognition by archaeon and Taq DNA polymerases
Article Snippet: .. Taq DNA polymerase (Amersham Pharmacia Biotech, Piscataway, NJ) and its F667Y derivative Thermo Sequenase (Amersham Pharmacia Biotech) naturally lack this activity, while the archaeon DNA polymerases have been genetically modified within the conserved exonuclease motif DIE to AIA to eliminate this activity ( – ). .. Vent and Deep Vent DNA polymerases were from New England Biolabs and Pfu DNA polymerase was from Stratagene (La Jolla, CA).

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    GE Healthcare phi29 dna polymerase
    Step-by-step schematic between MitoSV-seq and conventional methods. Conventional methods have focused primarily on brain homogenate, bulk cortical neurons (CNs), or single DA neurons isolated by MACS or LCM, followed by PCR amplification of mtDNA using site-specific primers designed mainly for the D-loop or major arc. Amplified PCR fragments are usually purified from agarose gels and processed for NGS library preparation and sequencing. MitoSV-seq benefits from FACS in characterising and isolating single cells from a heterogeneous pool of cells using cell-specific markers. Single cells are sorted and used directly for the amplification step. Concatemers from circular mtDNA are generated using RHs and <t>phi29</t> DNA polymerase in a rolling circle manner. Addition of our custom PC allows to us to first set up the analytical pipeline to find a known PC deletion. High-molecular-weight concatemers are digested and used for NGS library preparation and ultra-deep sequencing. MACS, magnetic cell separation; LCM, laser capture microdissection; PCR; polymerase chain reaction; FACS; fluorescence-activated cell sorting; RHs, random hexamers; RCA, rolling circle amplification; PC, positive control.
    Phi29 Dna Polymerase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phi29 dna polymerase/product/GE Healthcare
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    phi29 dna polymerase - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    85
    GE Healthcare phi29 polymerase amplification viral dna
    The human genomic <t>DNA</t> levels were reduced by pre-treatment. Comparing the effects of different pre-treatment methods on the level of human gDNA (measured as ß-actin), in a clinical sample. A HSV1 + skin lesion-sample was pre-treated with centrifugation and filtration only, or with centrifugation, filtration and DNase treatment. The ß-actin content was measured by real-time PCR. (A) Centrifugation+filtration: Ct = 22; centrifugation+filtration+DNase treatment: Ct = 32; ΔCt = 10 which equals a 1000-fold decrease in signal. Negative control was water used as a PCR control. (B) Time-course for <t>Phi29-amplification</t> of pre-treated clinical sample (centrifugation+filtration+DNase) using GenomiPhi (GE Healthcare). Fold increase in signal plotted versus amplification time for HSV1 and ß-actin.
    Phi29 Polymerase Amplification Viral Dna, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phi29 polymerase amplification viral dna/product/GE Healthcare
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phi29 polymerase amplification viral dna - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Step-by-step schematic between MitoSV-seq and conventional methods. Conventional methods have focused primarily on brain homogenate, bulk cortical neurons (CNs), or single DA neurons isolated by MACS or LCM, followed by PCR amplification of mtDNA using site-specific primers designed mainly for the D-loop or major arc. Amplified PCR fragments are usually purified from agarose gels and processed for NGS library preparation and sequencing. MitoSV-seq benefits from FACS in characterising and isolating single cells from a heterogeneous pool of cells using cell-specific markers. Single cells are sorted and used directly for the amplification step. Concatemers from circular mtDNA are generated using RHs and phi29 DNA polymerase in a rolling circle manner. Addition of our custom PC allows to us to first set up the analytical pipeline to find a known PC deletion. High-molecular-weight concatemers are digested and used for NGS library preparation and ultra-deep sequencing. MACS, magnetic cell separation; LCM, laser capture microdissection; PCR; polymerase chain reaction; FACS; fluorescence-activated cell sorting; RHs, random hexamers; RCA, rolling circle amplification; PC, positive control.

    Journal: EBioMedicine

    Article Title: Identification of unique and shared mitochondrial DNA mutations in neurodegeneration and cancer by single-cell mitochondrial DNA structural variation sequencing (MitoSV-seq)

    doi: 10.1016/j.ebiom.2020.102868

    Figure Lengend Snippet: Step-by-step schematic between MitoSV-seq and conventional methods. Conventional methods have focused primarily on brain homogenate, bulk cortical neurons (CNs), or single DA neurons isolated by MACS or LCM, followed by PCR amplification of mtDNA using site-specific primers designed mainly for the D-loop or major arc. Amplified PCR fragments are usually purified from agarose gels and processed for NGS library preparation and sequencing. MitoSV-seq benefits from FACS in characterising and isolating single cells from a heterogeneous pool of cells using cell-specific markers. Single cells are sorted and used directly for the amplification step. Concatemers from circular mtDNA are generated using RHs and phi29 DNA polymerase in a rolling circle manner. Addition of our custom PC allows to us to first set up the analytical pipeline to find a known PC deletion. High-molecular-weight concatemers are digested and used for NGS library preparation and ultra-deep sequencing. MACS, magnetic cell separation; LCM, laser capture microdissection; PCR; polymerase chain reaction; FACS; fluorescence-activated cell sorting; RHs, random hexamers; RCA, rolling circle amplification; PC, positive control.

    Article Snippet: RCA is performed on the PC and cell lysate using RHs, dNTPs (deoxyribonucleotide triphosphates), and Phi29 DNA polymerase to produce high-molecular-weight concatemers.

    Techniques: Isolation, Magnetic Cell Separation, Laser Capture Microdissection, Polymerase Chain Reaction, Amplification, Purification, Next-Generation Sequencing, Sequencing, FACS, Generated, Molecular Weight, Fluorescence, Positive Control

    The human genomic DNA levels were reduced by pre-treatment. Comparing the effects of different pre-treatment methods on the level of human gDNA (measured as ß-actin), in a clinical sample. A HSV1 + skin lesion-sample was pre-treated with centrifugation and filtration only, or with centrifugation, filtration and DNase treatment. The ß-actin content was measured by real-time PCR. (A) Centrifugation+filtration: Ct = 22; centrifugation+filtration+DNase treatment: Ct = 32; ΔCt = 10 which equals a 1000-fold decrease in signal. Negative control was water used as a PCR control. (B) Time-course for Phi29-amplification of pre-treated clinical sample (centrifugation+filtration+DNase) using GenomiPhi (GE Healthcare). Fold increase in signal plotted versus amplification time for HSV1 and ß-actin.

    Journal: PLoS ONE

    Article Title: The Microbial Detection Array Combined with Random Phi29-Amplification Used as a Diagnostic Tool for Virus Detection in Clinical Samples

    doi: 10.1371/journal.pone.0022631

    Figure Lengend Snippet: The human genomic DNA levels were reduced by pre-treatment. Comparing the effects of different pre-treatment methods on the level of human gDNA (measured as ß-actin), in a clinical sample. A HSV1 + skin lesion-sample was pre-treated with centrifugation and filtration only, or with centrifugation, filtration and DNase treatment. The ß-actin content was measured by real-time PCR. (A) Centrifugation+filtration: Ct = 22; centrifugation+filtration+DNase treatment: Ct = 32; ΔCt = 10 which equals a 1000-fold decrease in signal. Negative control was water used as a PCR control. (B) Time-course for Phi29-amplification of pre-treated clinical sample (centrifugation+filtration+DNase) using GenomiPhi (GE Healthcare). Fold increase in signal plotted versus amplification time for HSV1 and ß-actin.

    Article Snippet: Phi29 polymerase amplification Viral DNA was amplified by Phi29 polymerase amplification using GenomiPhi V2 Amplification kit (GE Healthcare) or Repli-g Midi kit (typical yield ∼40 µg from a 50 µl reaction, Qiagen), following manufacturer's protocols.

    Techniques: Centrifugation, Filtration, Real-time Polymerase Chain Reaction, Negative Control, Polymerase Chain Reaction, Amplification