phenylmethylsulfonyl fluoride  (Thermo Fisher)


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    Name:
    Orion Industrial Fluoride Sensing Half Cell Electrode for use
    Description:
    Ensure accurate results for your most important measurements with Thermo Scientific Orion Industrial Ion Selective Electrodes
    Catalog Number:
    100029
    Price:
    None
    Category:
    Instruments and Equipment
    Applications:
    Lab Equipment
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    Thermo Fisher phenylmethylsulfonyl fluoride
    Ensure accurate results for your most important measurements with Thermo Scientific Orion Industrial Ion Selective Electrodes
    https://www.bioz.com/result/phenylmethylsulfonyl fluoride/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylmethylsulfonyl fluoride - by Bioz Stars, 2021-03
    86/100 stars

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    other:

    Article Title: Degradation of 4-fluorophenol by Arthrobacter sp. strain IF1
    Article Snippet: Concentrations of free fluoride in the culture supernatants were measured with a fluoride electrode (model 96-09, Thermo Russell, Scotland).

    Article Title: In vitro remineralization of enamel by polymeric amorphous calcium phosphate composite: Quantitative micro-radiographic study
    Article Snippet: Solution fluoride content was determined by utilizing fluoride specific electrode (No. 100029; Thermo Fisher Scientific Inc., Waltham, MA, USA).

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    Thermo Fisher immunoprecipitation buffer
    RNAi target genes are specifically enriched in spliceosome immunoprecipitations. ( A , top ) Anti-Flag Western blot showing that Flag-RNP-3 is efficiently immunoprecipitated from transgenic C. elegans native extracts. ( Bottom ) Total RNA electrophoresed on a 1% agarose/TBE gel. ( B ) qRT–PCR from an RNP-3::3xFlag RNA <t>immunoprecipitation</t> to monitor which subunits of the spliceosome are coimmunoprecipitated with RNP-3 (as indicated by the structural RNA components of each subunit: U1, U2, U4, U5, and U6 snRNAs). Nonspliceosomal structural RNAs 5S and 5.8S rRNAs were examined as negative controls. ( C ) ddPCR analysis monitoring the enrichment of either non-RNAi target (“conserved”) or RNAi target genes in RNP-3::3xFlag immunoprecipitations. Two experimental replicates are plotted together to show general trends of either enrichment or depletion of each transcript in RNP-3 immunoprecipitations.
    Immunoprecipitation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 1 article reviews
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    86
    Thermo Fisher tris edta buffer
    Methyl mercury exposure results in cortical accumulation of insoluble Tdp-43. Cortical tissue from mice exposed, by ingestion, to 0 ppm (no treatment, N  = 4) and 5 ppm methyl mercuric (II) chloride (MeHg, N  = 7) was fractionated by solubility. The sarkosyl insoluble (A) and total lysate (B) (TE: <t>Tris-EDTA)</t> fractions were immunoblotted and probed for endogenous Tdp-43. Upon systemic MeHg exposure, sarkosyl insoluble Tdp-43 (C) and high molecular weight (HMW) Tdp-43 (D) is significantly increased. Mean ±SEM; unpaired two-tailed t test, * p  
    Tris Edta Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher pmsf
    Methyl mercury exposure results in cortical accumulation of insoluble Tdp-43. Cortical tissue from mice exposed, by ingestion, to 0 ppm (no treatment, N  = 4) and 5 ppm methyl mercuric (II) chloride (MeHg, N  = 7) was fractionated by solubility. The sarkosyl insoluble (A) and total lysate (B) (TE: <t>Tris-EDTA)</t> fractions were immunoblotted and probed for endogenous Tdp-43. Upon systemic MeHg exposure, sarkosyl insoluble Tdp-43 (C) and high molecular weight (HMW) Tdp-43 (D) is significantly increased. Mean ±SEM; unpaired two-tailed t test, * p  
    Pmsf, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
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    pmsf - by Bioz Stars, 2021-03
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    RNAi target genes are specifically enriched in spliceosome immunoprecipitations. ( A , top ) Anti-Flag Western blot showing that Flag-RNP-3 is efficiently immunoprecipitated from transgenic C. elegans native extracts. ( Bottom ) Total RNA electrophoresed on a 1% agarose/TBE gel. ( B ) qRT–PCR from an RNP-3::3xFlag RNA immunoprecipitation to monitor which subunits of the spliceosome are coimmunoprecipitated with RNP-3 (as indicated by the structural RNA components of each subunit: U1, U2, U4, U5, and U6 snRNAs). Nonspliceosomal structural RNAs 5S and 5.8S rRNAs were examined as negative controls. ( C ) ddPCR analysis monitoring the enrichment of either non-RNAi target (“conserved”) or RNAi target genes in RNP-3::3xFlag immunoprecipitations. Two experimental replicates are plotted together to show general trends of either enrichment or depletion of each transcript in RNP-3 immunoprecipitations.

    Journal: Genes & Development

    Article Title: The surveillance of pre-mRNA splicing is an early step in C. elegans RNAi of endogenous genes

    doi: 10.1101/gad.311514.118

    Figure Lengend Snippet: RNAi target genes are specifically enriched in spliceosome immunoprecipitations. ( A , top ) Anti-Flag Western blot showing that Flag-RNP-3 is efficiently immunoprecipitated from transgenic C. elegans native extracts. ( Bottom ) Total RNA electrophoresed on a 1% agarose/TBE gel. ( B ) qRT–PCR from an RNP-3::3xFlag RNA immunoprecipitation to monitor which subunits of the spliceosome are coimmunoprecipitated with RNP-3 (as indicated by the structural RNA components of each subunit: U1, U2, U4, U5, and U6 snRNAs). Nonspliceosomal structural RNAs 5S and 5.8S rRNAs were examined as negative controls. ( C ) ddPCR analysis monitoring the enrichment of either non-RNAi target (“conserved”) or RNAi target genes in RNP-3::3xFlag immunoprecipitations. Two experimental replicates are plotted together to show general trends of either enrichment or depletion of each transcript in RNP-3 immunoprecipitations.

    Article Snippet: Worm pellets were ground to a fine powder in liquid nitrogen and dissolved in 12 mL of ice-cold immunoprecipitation buffer (supplemented with 1 mM DTT, 1× PMSF, 0.02 U/µL RNase inhibitor [RiboLock; Thermo Fisher], complete protease inhibitors [EDTA-free; Roche]).

    Techniques: Western Blot, Immunoprecipitation, Transgenic Assay, Quantitative RT-PCR

    Methyl mercury exposure results in cortical accumulation of insoluble Tdp-43. Cortical tissue from mice exposed, by ingestion, to 0 ppm (no treatment, N  = 4) and 5 ppm methyl mercuric (II) chloride (MeHg, N  = 7) was fractionated by solubility. The sarkosyl insoluble (A) and total lysate (B) (TE: Tris-EDTA) fractions were immunoblotted and probed for endogenous Tdp-43. Upon systemic MeHg exposure, sarkosyl insoluble Tdp-43 (C) and high molecular weight (HMW) Tdp-43 (D) is significantly increased. Mean ±SEM; unpaired two-tailed t test, * p  

    Journal: Toxicological Sciences

    Article Title: Heavy Metal Neurotoxicants Induce ALS-Linked TDP-43 Pathology

    doi: 10.1093/toxsci/kfy267

    Figure Lengend Snippet: Methyl mercury exposure results in cortical accumulation of insoluble Tdp-43. Cortical tissue from mice exposed, by ingestion, to 0 ppm (no treatment, N  = 4) and 5 ppm methyl mercuric (II) chloride (MeHg, N  = 7) was fractionated by solubility. The sarkosyl insoluble (A) and total lysate (B) (TE: Tris-EDTA) fractions were immunoblotted and probed for endogenous Tdp-43. Upon systemic MeHg exposure, sarkosyl insoluble Tdp-43 (C) and high molecular weight (HMW) Tdp-43 (D) is significantly increased. Mean ±SEM; unpaired two-tailed t test, * p  

    Article Snippet: A total of ∼100 mg of frozen cortical tissue was lysed by bead mill (Thermo) homogenization in Tris-EDTA buffer (10 mM Tris pH8; 1 mM EDTA; 1 mM PMSF; 1× HALT PIC, and PhosSTOP [Roche]).

    Techniques: Mouse Assay, Solubility, Molecular Weight, Two Tailed Test