phenylmethylsulfonyl fluoride  (Nacalai)

 
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    Nacalai phenylmethylsulfonyl fluoride
    Coagulation occurred after parasite egress from iRBCs. a – c (i) Western blotting analysis of coagulation. a “Med” and “Sup” indicate PDS- or NCS-containing medium and PDS- or NCS-cultured supernatant, respectively. b After the addition of protease inhibitors ( PMSF <t>phenylmethylsulfonyl</t> fluoride, DE dabigatran etexilate). c “Lysate” indicates the isolated parasite lysate prepared using the precipitation method. The isolated parasite lysate prepared from iRBCs cultured in NCS-containing medium was added to plasma. (i) The absence of fibrinogen indicated coagulation, save for in the isolated parasite lysate (lanes 3 and 4). (ii) Visual confirmation of coagulation. Tubes, shown in lanes 3, 4, 5, and 6 in (i), containing 100 µL of the reaction mixture were rotated at a 90° angle onto their sides after completion of the coagulation reaction. All experiments were performed in triplicate and representative data are shown
    Phenylmethylsulfonyl Fluoride, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenylmethylsulfonyl fluoride/product/Nacalai
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylmethylsulfonyl fluoride - by Bioz Stars, 2021-03
    86/100 stars

    Images

    1) Product Images from "The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity"

    Article Title: The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity

    Journal: Malaria Journal

    doi: 10.1186/s12936-020-03229-1

    Coagulation occurred after parasite egress from iRBCs. a – c (i) Western blotting analysis of coagulation. a “Med” and “Sup” indicate PDS- or NCS-containing medium and PDS- or NCS-cultured supernatant, respectively. b After the addition of protease inhibitors ( PMSF phenylmethylsulfonyl fluoride, DE dabigatran etexilate). c “Lysate” indicates the isolated parasite lysate prepared using the precipitation method. The isolated parasite lysate prepared from iRBCs cultured in NCS-containing medium was added to plasma. (i) The absence of fibrinogen indicated coagulation, save for in the isolated parasite lysate (lanes 3 and 4). (ii) Visual confirmation of coagulation. Tubes, shown in lanes 3, 4, 5, and 6 in (i), containing 100 µL of the reaction mixture were rotated at a 90° angle onto their sides after completion of the coagulation reaction. All experiments were performed in triplicate and representative data are shown
    Figure Legend Snippet: Coagulation occurred after parasite egress from iRBCs. a – c (i) Western blotting analysis of coagulation. a “Med” and “Sup” indicate PDS- or NCS-containing medium and PDS- or NCS-cultured supernatant, respectively. b After the addition of protease inhibitors ( PMSF phenylmethylsulfonyl fluoride, DE dabigatran etexilate). c “Lysate” indicates the isolated parasite lysate prepared using the precipitation method. The isolated parasite lysate prepared from iRBCs cultured in NCS-containing medium was added to plasma. (i) The absence of fibrinogen indicated coagulation, save for in the isolated parasite lysate (lanes 3 and 4). (ii) Visual confirmation of coagulation. Tubes, shown in lanes 3, 4, 5, and 6 in (i), containing 100 µL of the reaction mixture were rotated at a 90° angle onto their sides after completion of the coagulation reaction. All experiments were performed in triplicate and representative data are shown

    Techniques Used: Coagulation, Western Blot, Cell Culture, Isolation

    2) Product Images from "Trypsin is a multifunctional factor in spermatogenesis"

    Article Title: Trypsin is a multifunctional factor in spermatogenesis

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.0907631106

    Effects of trypsin on spermatogenesis in the Japanese eel in vitro. ( A ) Effect of anti-trypsinogen antibody (Anti-TN) treatment on DHP-induced DNA replication of germ cells in a germ cell/somatic cell coculture system. IC, Initial control; KT, 10 ng/mL; DHP, 10 ng/mL; +, with anti-TN; -, without anti-TN. ( B ) Effect of serine protease inhibitor treatment on DHP-induced DNA replication of germ cells in a germ cell/somatic cell coculture system. PM, Phenylmethylsulfonyl fluoride (PMSF); AEB, 4-(2-Aminoethyl)-benzenesulfonyl fluoride (AEBSF). ( C ) Effect of various concentrations of porcine trypsin on germ cell/somatic cell cocultures.
    Figure Legend Snippet: Effects of trypsin on spermatogenesis in the Japanese eel in vitro. ( A ) Effect of anti-trypsinogen antibody (Anti-TN) treatment on DHP-induced DNA replication of germ cells in a germ cell/somatic cell coculture system. IC, Initial control; KT, 10 ng/mL; DHP, 10 ng/mL; +, with anti-TN; -, without anti-TN. ( B ) Effect of serine protease inhibitor treatment on DHP-induced DNA replication of germ cells in a germ cell/somatic cell coculture system. PM, Phenylmethylsulfonyl fluoride (PMSF); AEB, 4-(2-Aminoethyl)-benzenesulfonyl fluoride (AEBSF). ( C ) Effect of various concentrations of porcine trypsin on germ cell/somatic cell cocultures.

    Techniques Used: In Vitro, Protease Inhibitor

    Localization of trypsin in eel sperm. ( A ) Immunohistochemical analysis of whole cells using an anti-eel trypsinogen antibody. Trypsin localizes at the sperm membrane and within the mitochondria in the caput end of the sperm head. (Scale bar, 10 μm.) ( B ) Gelatin zymography assay and Western blot analysis of the eel sperm membrane with an anti-trypsinogen antibody (Anti-TN). PMSF − and + indicate with or without Phenylmethylsulfonyl fluoride, respectively. Arrowheads indicate molecular markers ( Left ), trypsin activity assayed by zymography, or signal detection by Western blot analysis using the anti-eel trypsinogen antibody.
    Figure Legend Snippet: Localization of trypsin in eel sperm. ( A ) Immunohistochemical analysis of whole cells using an anti-eel trypsinogen antibody. Trypsin localizes at the sperm membrane and within the mitochondria in the caput end of the sperm head. (Scale bar, 10 μm.) ( B ) Gelatin zymography assay and Western blot analysis of the eel sperm membrane with an anti-trypsinogen antibody (Anti-TN). PMSF − and + indicate with or without Phenylmethylsulfonyl fluoride, respectively. Arrowheads indicate molecular markers ( Left ), trypsin activity assayed by zymography, or signal detection by Western blot analysis using the anti-eel trypsinogen antibody.

    Techniques Used: Immunohistochemistry, Zymography Assay, Western Blot, Activity Assay, Zymography

    3) Product Images from "The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity"

    Article Title: The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity

    Journal: Malaria Journal

    doi: 10.1186/s12936-020-03229-1

    Coagulation occurred after parasite egress from iRBCs. a – c (i) Western blotting analysis of coagulation. a “Med” and “Sup” indicate PDS- or NCS-containing medium and PDS- or NCS-cultured supernatant, respectively. b After the addition of protease inhibitors ( PMSF phenylmethylsulfonyl fluoride, DE dabigatran etexilate). c “Lysate” indicates the isolated parasite lysate prepared using the precipitation method. The isolated parasite lysate prepared from iRBCs cultured in NCS-containing medium was added to plasma. (i) The absence of fibrinogen indicated coagulation, save for in the isolated parasite lysate (lanes 3 and 4). (ii) Visual confirmation of coagulation. Tubes, shown in lanes 3, 4, 5, and 6 in (i), containing 100 µL of the reaction mixture were rotated at a 90° angle onto their sides after completion of the coagulation reaction. All experiments were performed in triplicate and representative data are shown
    Figure Legend Snippet: Coagulation occurred after parasite egress from iRBCs. a – c (i) Western blotting analysis of coagulation. a “Med” and “Sup” indicate PDS- or NCS-containing medium and PDS- or NCS-cultured supernatant, respectively. b After the addition of protease inhibitors ( PMSF phenylmethylsulfonyl fluoride, DE dabigatran etexilate). c “Lysate” indicates the isolated parasite lysate prepared using the precipitation method. The isolated parasite lysate prepared from iRBCs cultured in NCS-containing medium was added to plasma. (i) The absence of fibrinogen indicated coagulation, save for in the isolated parasite lysate (lanes 3 and 4). (ii) Visual confirmation of coagulation. Tubes, shown in lanes 3, 4, 5, and 6 in (i), containing 100 µL of the reaction mixture were rotated at a 90° angle onto their sides after completion of the coagulation reaction. All experiments were performed in triplicate and representative data are shown

    Techniques Used: Coagulation, Western Blot, Cell Culture, Isolation

    Related Articles

    Western Blot:

    Article Title: Cytoprotective role of the fatty acid binding protein 4 against oxidative and endoplasmic reticulum stress in 3T3-L1 adipocytes
    Article Snippet: PCR products were subjected to 2% agarose gel electrophoresis and the gels were viewed by means of an Image Analyzer (model LAS-4000mini, GE Healthcare, Tokyo, Japan) after staining with ethidium bromide. .. 4.5 Western blotting The cells were lysed with buffer containing 1% Triton X-100, 50 mM Tris–HCl (pH 7.5), 200 mM NaCl, 2 mM phenylmethylsulfonyl fluoride (PMSF), 1 × protease inhibitor cocktail (Nacalai tesque, Kyoto, Japan), and 1 × PhosSTOP Phosphatase inhibitor cocktail (Roche, Mannheim, Germany). .. The protein concentrations in cell lysates were determined with a BCA Protein Assay Kit (Pierce, Rockford, IL, USA).

    Protease Inhibitor:

    Article Title: Cytoprotective role of the fatty acid binding protein 4 against oxidative and endoplasmic reticulum stress in 3T3-L1 adipocytes
    Article Snippet: PCR products were subjected to 2% agarose gel electrophoresis and the gels were viewed by means of an Image Analyzer (model LAS-4000mini, GE Healthcare, Tokyo, Japan) after staining with ethidium bromide. .. 4.5 Western blotting The cells were lysed with buffer containing 1% Triton X-100, 50 mM Tris–HCl (pH 7.5), 200 mM NaCl, 2 mM phenylmethylsulfonyl fluoride (PMSF), 1 × protease inhibitor cocktail (Nacalai tesque, Kyoto, Japan), and 1 × PhosSTOP Phosphatase inhibitor cocktail (Roche, Mannheim, Germany). .. The protein concentrations in cell lysates were determined with a BCA Protein Assay Kit (Pierce, Rockford, IL, USA).

    Article Title: Prolyl Isomerase Pin1 Directly Regulates Calcium/Calmodulin-Dependent Protein Kinase II Activity in Mouse Brains
    Article Snippet: MultiGauge software (Fujifilm) was used to measure the bands semi-quantitatively. .. CaMKII Kinase Assay Mouse brains were lysed in extraction buffer (100 mM piperazine-N,N′-bis(2-ethanesulfonic acid) (PIPES), pH 6.9; 10 mM ethylenediamine tetraacetic acid (EDTA); 10 mM ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA); 1 mM phenylmethylsulfonyl fluoride (PMSF); 2 mM Na3 VO4 ; 2 mM NaF; 2 mM dithiothreitol (DTT); protease inhibitor cocktail (Nacalai Tesque Inc.). .. The N2a cells were transiently transfected with the flag-CaMKIIα and Pin1 (WT, W34A, R68/69A ) plasmids.

    Article Title: Cryo-EM structures of SERCA2b reveal the mechanism of regulation by the luminal extension tail
    Article Snippet: Purification of SERCA2b and SERCA2bT1032stopAll purification steps were performed at 4°C. .. Harvested cells were lysed using a Dounce Homogenizer and solubilized in a buffer containing 50 mM HEPES-NaOH (pH 7.0), 100 mM NaCl, 20% glycerol, 1 mM CaCl2, 1 mM MgCl2, 1 mM dithiothreitol (DTT), 1 mM phenylmethylsulfonyl fluoride (PMSF), and 1/100 Protease Inhibitor Cocktail (Nacalai). .. After homogenization, 1% (w/v) DDM was added to solubilize the membrane fraction.

    Article Title: Prolyl Isomerase Pin1 Directly Regulates Calcium/Calmodulin-Dependent Protein Kinase II Activity in Mouse Brains
    Article Snippet: MultiGauge software (Fujifilm) was used to measure the bands semi-quantitatively. .. Mouse brains were lysed in extraction buffer (100 mM piperazine-N,N′-bis(2-ethanesulfonic acid) (PIPES), pH 6.9; 10 mM ethylenediamine tetraacetic acid (EDTA); 10 mM ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA); 1 mM phenylmethylsulfonyl fluoride (PMSF); 2 mM Na3 VO4 ; 2 mM NaF; 2 mM dithiothreitol (DTT); protease inhibitor cocktail (Nacalai Tesque Inc.). .. The N2a cells were transiently transfected with the flag-CaMKIIα and Pin1 ( WT, W34A, R68/69A ) plasmids.

    Kinase Assay:

    Article Title: Prolyl Isomerase Pin1 Directly Regulates Calcium/Calmodulin-Dependent Protein Kinase II Activity in Mouse Brains
    Article Snippet: MultiGauge software (Fujifilm) was used to measure the bands semi-quantitatively. .. CaMKII Kinase Assay Mouse brains were lysed in extraction buffer (100 mM piperazine-N,N′-bis(2-ethanesulfonic acid) (PIPES), pH 6.9; 10 mM ethylenediamine tetraacetic acid (EDTA); 10 mM ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA); 1 mM phenylmethylsulfonyl fluoride (PMSF); 2 mM Na3 VO4 ; 2 mM NaF; 2 mM dithiothreitol (DTT); protease inhibitor cocktail (Nacalai Tesque Inc.). .. The N2a cells were transiently transfected with the flag-CaMKIIα and Pin1 (WT, W34A, R68/69A ) plasmids.

    Purification:

    Article Title: Expression of a functional oxygen-labile nitrogenase component in the mitochondrial matrix of aerobically grown yeast
    Article Snippet: The cells were collected under anaerobic conditions 20 h after galactose addition by using hollow fiber filtration followed by centrifugation at 4 °C and 4,000 r.c.f. for 5 min. .. yNifH purification S. cerevisiae cells (strains GF2, GF8, GF9, GF11, GF12 and GF13) were resuspended in anaerobic lysis buffer containing 100 mM Tris-HCl pH 8.0, 100 mM NaCl, 1 mM phenylmethylsulfonyl fluoride (PMSF), 1 μg ml−1 leupeptin, 2 mM sodium dithionite (DTH), 5 μg ml−1 DNaseI, and 1 mg g−1 cell of Zymolyase-20T (Nacalai Tesque). ..

    Lysis:

    Article Title: Expression of a functional oxygen-labile nitrogenase component in the mitochondrial matrix of aerobically grown yeast
    Article Snippet: The cells were collected under anaerobic conditions 20 h after galactose addition by using hollow fiber filtration followed by centrifugation at 4 °C and 4,000 r.c.f. for 5 min. .. yNifH purification S. cerevisiae cells (strains GF2, GF8, GF9, GF11, GF12 and GF13) were resuspended in anaerobic lysis buffer containing 100 mM Tris-HCl pH 8.0, 100 mM NaCl, 1 mM phenylmethylsulfonyl fluoride (PMSF), 1 μg ml−1 leupeptin, 2 mM sodium dithionite (DTH), 5 μg ml−1 DNaseI, and 1 mg g−1 cell of Zymolyase-20T (Nacalai Tesque). ..

    other:

    Article Title: A novel protocol for the preparation of active recombinant human pancreatic lipase from Escherichia coli
    Article Snippet: Phenylmethylsulfonyl fluoride (PMSF) was purchased from Nacalai Tesque, Inc. (Kyoto, Japan).

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    • phenylmethylsulfonyl fluoride  (Nacalai)
    86
    Nacalai phenylmethylsulfonyl fluoride
    Coagulation occurred after parasite egress from iRBCs. a – c (i) Western blotting analysis of coagulation. a “Med” and “Sup” indicate PDS- or NCS-containing medium and PDS- or NCS-cultured supernatant, respectively. b After the addition of protease inhibitors ( PMSF <t>phenylmethylsulfonyl</t> fluoride, DE dabigatran etexilate). c “Lysate” indicates the isolated parasite lysate prepared using the precipitation method. The isolated parasite lysate prepared from iRBCs cultured in NCS-containing medium was added to plasma. (i) The absence of fibrinogen indicated coagulation, save for in the isolated parasite lysate (lanes 3 and 4). (ii) Visual confirmation of coagulation. Tubes, shown in lanes 3, 4, 5, and 6 in (i), containing 100 µL of the reaction mixture were rotated at a 90° angle onto their sides after completion of the coagulation reaction. All experiments were performed in triplicate and representative data are shown
    Phenylmethylsulfonyl Fluoride, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenylmethylsulfonyl fluoride/product/Nacalai
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylmethylsulfonyl fluoride - by Bioz Stars, 2021-03
    86/100 stars
    		  Buy from Supplier
    m phenylmethylsulfonyl fluoride  (Nacalai)
    86
    Nacalai m phenylmethylsulfonyl fluoride
    Coagulation occurred after parasite egress from iRBCs. a – c (i) Western blotting analysis of coagulation. a “Med” and “Sup” indicate PDS- or NCS-containing medium and PDS- or NCS-cultured supernatant, respectively. b After the addition of protease inhibitors ( PMSF <t>phenylmethylsulfonyl</t> fluoride, DE dabigatran etexilate). c “Lysate” indicates the isolated parasite lysate prepared using the precipitation method. The isolated parasite lysate prepared from iRBCs cultured in NCS-containing medium was added to plasma. (i) The absence of fibrinogen indicated coagulation, save for in the isolated parasite lysate (lanes 3 and 4). (ii) Visual confirmation of coagulation. Tubes, shown in lanes 3, 4, 5, and 6 in (i), containing 100 µL of the reaction mixture were rotated at a 90° angle onto their sides after completion of the coagulation reaction. All experiments were performed in triplicate and representative data are shown
    M Phenylmethylsulfonyl Fluoride, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m phenylmethylsulfonyl fluoride/product/Nacalai
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m phenylmethylsulfonyl fluoride - by Bioz Stars, 2021-03
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Coagulation occurred after parasite egress from iRBCs. a – c (i) Western blotting analysis of coagulation. a “Med” and “Sup” indicate PDS- or NCS-containing medium and PDS- or NCS-cultured supernatant, respectively. b After the addition of protease inhibitors ( PMSF phenylmethylsulfonyl fluoride, DE dabigatran etexilate). c “Lysate” indicates the isolated parasite lysate prepared using the precipitation method. The isolated parasite lysate prepared from iRBCs cultured in NCS-containing medium was added to plasma. (i) The absence of fibrinogen indicated coagulation, save for in the isolated parasite lysate (lanes 3 and 4). (ii) Visual confirmation of coagulation. Tubes, shown in lanes 3, 4, 5, and 6 in (i), containing 100 µL of the reaction mixture were rotated at a 90° angle onto their sides after completion of the coagulation reaction. All experiments were performed in triplicate and representative data are shown

    Journal: Malaria Journal

    Article Title: The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity

    doi: 10.1186/s12936-020-03229-1

    Figure Lengend Snippet: Coagulation occurred after parasite egress from iRBCs. a – c (i) Western blotting analysis of coagulation. a “Med” and “Sup” indicate PDS- or NCS-containing medium and PDS- or NCS-cultured supernatant, respectively. b After the addition of protease inhibitors ( PMSF phenylmethylsulfonyl fluoride, DE dabigatran etexilate). c “Lysate” indicates the isolated parasite lysate prepared using the precipitation method. The isolated parasite lysate prepared from iRBCs cultured in NCS-containing medium was added to plasma. (i) The absence of fibrinogen indicated coagulation, save for in the isolated parasite lysate (lanes 3 and 4). (ii) Visual confirmation of coagulation. Tubes, shown in lanes 3, 4, 5, and 6 in (i), containing 100 µL of the reaction mixture were rotated at a 90° angle onto their sides after completion of the coagulation reaction. All experiments were performed in triplicate and representative data are shown

    Article Snippet: Phenylmethylsulfonyl fluoride

    Techniques: Coagulation, Western Blot, Cell Culture, Isolation

    Effects of trypsin on spermatogenesis in the Japanese eel in vitro. ( A ) Effect of anti-trypsinogen antibody (Anti-TN) treatment on DHP-induced DNA replication of germ cells in a germ cell/somatic cell coculture system. IC, Initial control; KT, 10 ng/mL; DHP, 10 ng/mL; +, with anti-TN; -, without anti-TN. ( B ) Effect of serine protease inhibitor treatment on DHP-induced DNA replication of germ cells in a germ cell/somatic cell coculture system. PM, Phenylmethylsulfonyl fluoride (PMSF); AEB, 4-(2-Aminoethyl)-benzenesulfonyl fluoride (AEBSF). ( C ) Effect of various concentrations of porcine trypsin on germ cell/somatic cell cocultures.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Trypsin is a multifunctional factor in spermatogenesis

    doi: 10.1073/pnas.0907631106

    Figure Lengend Snippet: Effects of trypsin on spermatogenesis in the Japanese eel in vitro. ( A ) Effect of anti-trypsinogen antibody (Anti-TN) treatment on DHP-induced DNA replication of germ cells in a germ cell/somatic cell coculture system. IC, Initial control; KT, 10 ng/mL; DHP, 10 ng/mL; +, with anti-TN; -, without anti-TN. ( B ) Effect of serine protease inhibitor treatment on DHP-induced DNA replication of germ cells in a germ cell/somatic cell coculture system. PM, Phenylmethylsulfonyl fluoride (PMSF); AEB, 4-(2-Aminoethyl)-benzenesulfonyl fluoride (AEBSF). ( C ) Effect of various concentrations of porcine trypsin on germ cell/somatic cell cocultures.

    Article Snippet: To further understand the function of trypsin during spermatogenesis, these cultures were exposed to porcine trypsin (Nacalai Tesque), Phenylmethylsulfonyl fluoride (PMSF; Nacalai Tesque), 4-(2-Aminoethyl)-benzenesulfonyl fluoride (AEBSF; Nacalai Tesque) or anti-eel trypsinogen antibodies.

    Techniques: In Vitro, Protease Inhibitor

    Localization of trypsin in eel sperm. ( A ) Immunohistochemical analysis of whole cells using an anti-eel trypsinogen antibody. Trypsin localizes at the sperm membrane and within the mitochondria in the caput end of the sperm head. (Scale bar, 10 μm.) ( B ) Gelatin zymography assay and Western blot analysis of the eel sperm membrane with an anti-trypsinogen antibody (Anti-TN). PMSF − and + indicate with or without Phenylmethylsulfonyl fluoride, respectively. Arrowheads indicate molecular markers ( Left ), trypsin activity assayed by zymography, or signal detection by Western blot analysis using the anti-eel trypsinogen antibody.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Trypsin is a multifunctional factor in spermatogenesis

    doi: 10.1073/pnas.0907631106

    Figure Lengend Snippet: Localization of trypsin in eel sperm. ( A ) Immunohistochemical analysis of whole cells using an anti-eel trypsinogen antibody. Trypsin localizes at the sperm membrane and within the mitochondria in the caput end of the sperm head. (Scale bar, 10 μm.) ( B ) Gelatin zymography assay and Western blot analysis of the eel sperm membrane with an anti-trypsinogen antibody (Anti-TN). PMSF − and + indicate with or without Phenylmethylsulfonyl fluoride, respectively. Arrowheads indicate molecular markers ( Left ), trypsin activity assayed by zymography, or signal detection by Western blot analysis using the anti-eel trypsinogen antibody.

    Article Snippet: To further understand the function of trypsin during spermatogenesis, these cultures were exposed to porcine trypsin (Nacalai Tesque), Phenylmethylsulfonyl fluoride (PMSF; Nacalai Tesque), 4-(2-Aminoethyl)-benzenesulfonyl fluoride (AEBSF; Nacalai Tesque) or anti-eel trypsinogen antibodies.

    Techniques: Immunohistochemistry, Zymography Assay, Western Blot, Activity Assay, Zymography