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Amresco phenylmethanesulfonyl fluoride
Effect of CdTe QDs of different sizes on autophagy in yeast at different time points. Notes: Cells transformed with a plasmid encoding GFP-Atg8 were treated with ( A ) 50 nmol/L CdTe QDs or 100 ng/mL rapamycin or ( B ) <t>phenylmethanesulfonyl</t> fluoride (PMSF), which was added at 0 hour and 16 hours to reach a final concentration of 2 mmol/L, and photographed at 4 hours, 8 hours, 16 hours, and 42 hours using fluorescence microscopy. Scale bars: 5 µm. Abbreviations: CdTe QDs, cadmium telluride quantum dots; G-CdTe, green-emitting CdTe; O-CdTe, orange-emitting CdTe.
Phenylmethanesulfonyl Fluoride, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Inhibition of autophagy contributes to the toxicity of cadmium telluride quantum dots in Saccharomyces cerevisiae"

Article Title: Inhibition of autophagy contributes to the toxicity of cadmium telluride quantum dots in Saccharomyces cerevisiae

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S108636

Effect of CdTe QDs of different sizes on autophagy in yeast at different time points. Notes: Cells transformed with a plasmid encoding GFP-Atg8 were treated with ( A ) 50 nmol/L CdTe QDs or 100 ng/mL rapamycin or ( B ) phenylmethanesulfonyl fluoride (PMSF), which was added at 0 hour and 16 hours to reach a final concentration of 2 mmol/L, and photographed at 4 hours, 8 hours, 16 hours, and 42 hours using fluorescence microscopy. Scale bars: 5 µm. Abbreviations: CdTe QDs, cadmium telluride quantum dots; G-CdTe, green-emitting CdTe; O-CdTe, orange-emitting CdTe.
Figure Legend Snippet: Effect of CdTe QDs of different sizes on autophagy in yeast at different time points. Notes: Cells transformed with a plasmid encoding GFP-Atg8 were treated with ( A ) 50 nmol/L CdTe QDs or 100 ng/mL rapamycin or ( B ) phenylmethanesulfonyl fluoride (PMSF), which was added at 0 hour and 16 hours to reach a final concentration of 2 mmol/L, and photographed at 4 hours, 8 hours, 16 hours, and 42 hours using fluorescence microscopy. Scale bars: 5 µm. Abbreviations: CdTe QDs, cadmium telluride quantum dots; G-CdTe, green-emitting CdTe; O-CdTe, orange-emitting CdTe.

Techniques Used: Transformation Assay, Plasmid Preparation, Concentration Assay, Fluorescence, Microscopy

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Concentration Assay:

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Article Snippet: Protein expression and purification ApoO protein was expressed in BL21 (DE3) cells, which were grown on Luria-Bertani medium with 50 μg/ml ampicillin. .. Isopropyl β-d-thiogalactoside (IPTG) was added to a final concentration of 0.1 mM to induce protein expression, and the culture was incubated at 37°C with shaking for 4 h. Cells were pelleted at 5,000 g for 15 min at 4°C and resuspended in 40 ml of ice-cold imidazole buffer (20 mM imidazole, 500 mM NaCl, and 20 mM phosphate buffer, pH 6.6) supplemented with 1 mM phenylmethanesulfonyl fluoride (Amresco), 1 mM MgCl2, and 0.2 mg/ml lysozyme. .. The cells were disrupted by sonication and insoluble proteins were pelleted by centrifugation at 5,000 g for 30 min at 4°C.

Expressing:

Article Title: Plasma apolipoprotein O level increased in the patients with acute coronary syndrome
Article Snippet: Protein expression and purification ApoO protein was expressed in BL21 (DE3) cells, which were grown on Luria-Bertani medium with 50 μg/ml ampicillin. .. Isopropyl β-d-thiogalactoside (IPTG) was added to a final concentration of 0.1 mM to induce protein expression, and the culture was incubated at 37°C with shaking for 4 h. Cells were pelleted at 5,000 g for 15 min at 4°C and resuspended in 40 ml of ice-cold imidazole buffer (20 mM imidazole, 500 mM NaCl, and 20 mM phosphate buffer, pH 6.6) supplemented with 1 mM phenylmethanesulfonyl fluoride (Amresco), 1 mM MgCl2, and 0.2 mg/ml lysozyme. .. The cells were disrupted by sonication and insoluble proteins were pelleted by centrifugation at 5,000 g for 30 min at 4°C.

Incubation:

Article Title: Plasma apolipoprotein O level increased in the patients with acute coronary syndrome
Article Snippet: Protein expression and purification ApoO protein was expressed in BL21 (DE3) cells, which were grown on Luria-Bertani medium with 50 μg/ml ampicillin. .. Isopropyl β-d-thiogalactoside (IPTG) was added to a final concentration of 0.1 mM to induce protein expression, and the culture was incubated at 37°C with shaking for 4 h. Cells were pelleted at 5,000 g for 15 min at 4°C and resuspended in 40 ml of ice-cold imidazole buffer (20 mM imidazole, 500 mM NaCl, and 20 mM phosphate buffer, pH 6.6) supplemented with 1 mM phenylmethanesulfonyl fluoride (Amresco), 1 mM MgCl2, and 0.2 mg/ml lysozyme. .. The cells were disrupted by sonication and insoluble proteins were pelleted by centrifugation at 5,000 g for 30 min at 4°C.

Article Title: Vitamin A Supplementation in Early Life Enhances the Intestinal Immune Response of Rats with Gestational Vitamin A Deficiency by Increasing the Number of Immune Cells
Article Snippet: Detection of IgA in the feces The concentration of secretory IgA (sIgA) in the intestine was measured using an ELISA kit (Bethyl Lab, Inc., USA) according to the manufacturer's instructions. .. Briefly, according to the method described by Frossard , 250 mg of feces obtained from the ileocecum of rats was resuspended and incubated in 1 ml of 0.01 M PBS containing 1% fetal bovine serum (FBS) and 0.1 mM phenylmethanesulfonyl fluoride (PMSF, Solarbio, Amresco) for 1 hr at room temperature. .. The concentration of total sIgA in the supernatant was determined using an ELISA kit.

Western Blot:

Article Title: Novel noninvasive quantification of penile corpus cavernosum lesions in hyperlipidemia-induced erectile dysfunction in rabbits by two-dimensional shear-wave elastography
Article Snippet: The slides were photographed using a Nikon Microphot-FXA microscope (Nikon Co. Ltd., Kogaku, Tokyo, Japan). .. Western blot analysis Frozen samples were washed three times in PBS (Beyotime Biotechnology Co. Ltd., Shanghai, China), cut into small pieces, then suspended in 1 mmol l−1 phenylmethanesulfonyl fluoride (PMSF; Amresco Inc., Huston, TX, USA) and radioimmunoprecipitation (RIPA) buffer (Beyotime Biotechnology Co. Ltd.) on ice. .. Thirty minutes later, the lysed homogenate was centrifuged at 10 000 g for 5 min and the supernatant was collected for protein measurement using a BCA kit (Beyotime Biotechnology Co. Ltd.).

Activation Assay:

Article Title: Identification and Characterization of Paramyosin from Cyst Wall of Metacercariae Implicated Protective Efficacy against Clonorchis sinensis Infection
Article Snippet: 0.001% trypsin (Promega, Wisconsin,USA) in physiological saline was employed as excystation stimulus in vitro . .. After activation, cyst wall of metacercariae was immediately collected in RIPA lysis buffer (Amresco, Solon, USA), added with 1 mM Phenylmethanesulfonyl fluoride (PMSF, Sigma, St. Louis, USA). .. The cyst wall was placed on ice in RIPA lysis buffer and then subjected to centrifugation at 8,000 g at 4°C for 15 min to remove the sediment, 4-fold cold acetone (containing 0.07% β-mercaptoethanol) was added to the supernatant and stored at −20°C overnight.

Lysis:

Article Title: Identification and Characterization of Paramyosin from Cyst Wall of Metacercariae Implicated Protective Efficacy against Clonorchis sinensis Infection
Article Snippet: 0.001% trypsin (Promega, Wisconsin,USA) in physiological saline was employed as excystation stimulus in vitro . .. After activation, cyst wall of metacercariae was immediately collected in RIPA lysis buffer (Amresco, Solon, USA), added with 1 mM Phenylmethanesulfonyl fluoride (PMSF, Sigma, St. Louis, USA). .. The cyst wall was placed on ice in RIPA lysis buffer and then subjected to centrifugation at 8,000 g at 4°C for 15 min to remove the sediment, 4-fold cold acetone (containing 0.07% β-mercaptoethanol) was added to the supernatant and stored at −20°C overnight.

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  • 86
    Amresco phenylmethanesulfonyl fluoride
    Effect of CdTe QDs of different sizes on autophagy in yeast at different time points. Notes: Cells transformed with a plasmid encoding GFP-Atg8 were treated with ( A ) 50 nmol/L CdTe QDs or 100 ng/mL rapamycin or ( B ) <t>phenylmethanesulfonyl</t> fluoride (PMSF), which was added at 0 hour and 16 hours to reach a final concentration of 2 mmol/L, and photographed at 4 hours, 8 hours, 16 hours, and 42 hours using fluorescence microscopy. Scale bars: 5 µm. Abbreviations: CdTe QDs, cadmium telluride quantum dots; G-CdTe, green-emitting CdTe; O-CdTe, orange-emitting CdTe.
    Phenylmethanesulfonyl Fluoride, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenylmethanesulfonyl fluoride/product/Amresco
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylmethanesulfonyl fluoride - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Amresco protease inhibitor phenylmethanesulfonyl fluoride
    Effect of CdTe QDs of different sizes on autophagy in yeast at different time points. Notes: Cells transformed with a plasmid encoding GFP-Atg8 were treated with ( A ) 50 nmol/L CdTe QDs or 100 ng/mL rapamycin or ( B ) <t>phenylmethanesulfonyl</t> fluoride (PMSF), which was added at 0 hour and 16 hours to reach a final concentration of 2 mmol/L, and photographed at 4 hours, 8 hours, 16 hours, and 42 hours using fluorescence microscopy. Scale bars: 5 µm. Abbreviations: CdTe QDs, cadmium telluride quantum dots; G-CdTe, green-emitting CdTe; O-CdTe, orange-emitting CdTe.
    Protease Inhibitor Phenylmethanesulfonyl Fluoride, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease inhibitor phenylmethanesulfonyl fluoride/product/Amresco
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protease inhibitor phenylmethanesulfonyl fluoride - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Amresco phenylmethyl sulfonyl fluoride
    Effect of CdTe QDs of different sizes on autophagy in yeast at different time points. Notes: Cells transformed with a plasmid encoding GFP-Atg8 were treated with ( A ) 50 nmol/L CdTe QDs or 100 ng/mL rapamycin or ( B ) <t>phenylmethanesulfonyl</t> fluoride (PMSF), which was added at 0 hour and 16 hours to reach a final concentration of 2 mmol/L, and photographed at 4 hours, 8 hours, 16 hours, and 42 hours using fluorescence microscopy. Scale bars: 5 µm. Abbreviations: CdTe QDs, cadmium telluride quantum dots; G-CdTe, green-emitting CdTe; O-CdTe, orange-emitting CdTe.
    Phenylmethyl Sulfonyl Fluoride, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenylmethyl sulfonyl fluoride/product/Amresco
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylmethyl sulfonyl fluoride - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Amresco phenylmethanesulfonyl fluoride pmsf
    Effect of CdTe QDs of different sizes on autophagy in yeast at different time points. Notes: Cells transformed with a plasmid encoding GFP-Atg8 were treated with ( A ) 50 nmol/L CdTe QDs or 100 ng/mL rapamycin or ( B ) <t>phenylmethanesulfonyl</t> fluoride (PMSF), which was added at 0 hour and 16 hours to reach a final concentration of 2 mmol/L, and photographed at 4 hours, 8 hours, 16 hours, and 42 hours using fluorescence microscopy. Scale bars: 5 µm. Abbreviations: CdTe QDs, cadmium telluride quantum dots; G-CdTe, green-emitting CdTe; O-CdTe, orange-emitting CdTe.
    Phenylmethanesulfonyl Fluoride Pmsf, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenylmethanesulfonyl fluoride pmsf/product/Amresco
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylmethanesulfonyl fluoride pmsf - by Bioz Stars, 2021-03
    86/100 stars
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    Effect of CdTe QDs of different sizes on autophagy in yeast at different time points. Notes: Cells transformed with a plasmid encoding GFP-Atg8 were treated with ( A ) 50 nmol/L CdTe QDs or 100 ng/mL rapamycin or ( B ) phenylmethanesulfonyl fluoride (PMSF), which was added at 0 hour and 16 hours to reach a final concentration of 2 mmol/L, and photographed at 4 hours, 8 hours, 16 hours, and 42 hours using fluorescence microscopy. Scale bars: 5 µm. Abbreviations: CdTe QDs, cadmium telluride quantum dots; G-CdTe, green-emitting CdTe; O-CdTe, orange-emitting CdTe.

    Journal: International Journal of Nanomedicine

    Article Title: Inhibition of autophagy contributes to the toxicity of cadmium telluride quantum dots in Saccharomyces cerevisiae

    doi: 10.2147/IJN.S108636

    Figure Lengend Snippet: Effect of CdTe QDs of different sizes on autophagy in yeast at different time points. Notes: Cells transformed with a plasmid encoding GFP-Atg8 were treated with ( A ) 50 nmol/L CdTe QDs or 100 ng/mL rapamycin or ( B ) phenylmethanesulfonyl fluoride (PMSF), which was added at 0 hour and 16 hours to reach a final concentration of 2 mmol/L, and photographed at 4 hours, 8 hours, 16 hours, and 42 hours using fluorescence microscopy. Scale bars: 5 µm. Abbreviations: CdTe QDs, cadmium telluride quantum dots; G-CdTe, green-emitting CdTe; O-CdTe, orange-emitting CdTe.

    Article Snippet: Phenylmethanesulfonyl fluoride was obtained from Amresco (Solon, OH, USA).

    Techniques: Transformation Assay, Plasmid Preparation, Concentration Assay, Fluorescence, Microscopy