phenylmethanesulfonyl fluoride solution  (Millipore)


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  • 99
    Name:
    Phenylmethanesulfonyl fluoride solution
    Description:

    Catalog Number:
    93482
    Price:
    None
    Applications:
    Phenylmethanesulfonyl fluoride solution has been used as a component in the cell lysate buffer in breast adenocarcinoma cell line, fibrosarcoma cell line and D5 hindbrain and spinal cord neural progenitors.
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    Structured Review

    Millipore phenylmethanesulfonyl fluoride solution
    Phenylmethanesulfonyl fluoride solution

    https://www.bioz.com/result/phenylmethanesulfonyl fluoride solution/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylmethanesulfonyl fluoride solution - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Latent myostatin has significant activity and this activity is controlled more efficiently by WFIKKN1 than by WFIKKN2
    Article Snippet: .. Protein precipitates were removed by centrifugation at 3500 g for 15 min and the protein solution was dialyzed against 3 × 3 L of 100 mm Tris/HCl, 100 μm phenylmethanesulfonyl fluoride and 0.005% 2-mercaptoethanol (pH 8.0) at 4 °C for 36 h. Precipitates were removed by centrifugation at 3500 g for 15 min and the protein solution was then concentrated on an Amicon stirred ultrafiltration cell (EMD Millipore, Billerica, MA USA) and applied to a 20-mL Strep-Tactin Sepharose column. ..

    Radio Immunoprecipitation:

    Article Title: An effector of a necrotrophic fungal pathogen targets the calcium‐sensing receptor in chloroplasts to inhibit host resistance, et al. An effector of a necrotrophic fungal pathogen targets the calcium sensing receptor in chloroplasts to inhibit host resistance
    Article Snippet: .. 4.3 Protein extraction, western blot, IP, and LC‐MS/MS assays For protein extraction, plant tissue was ground in liquid nitrogen and mixed with an equal volume of radio immunoprecipitation assay (RIPA) lysis buffer (Beyotime) with 1 mM phenylmethanesulfonyl fluoride and 1% proteinase inhibitor cocktail (Sigma), then incubated on ice for 30 min and centrifuged at 13,000 × g for 15 min at 4 °C. .. The supernatant was transferred to a new tube and boiled in sodium dodecyl sulphate (SDS) loading buffer for 5 min. Proteins were separated by SDS‐polyacrylamide gel electrophoresis (PAGE) (12%) followed by electroblotting onto a 0.22 μm polyvinylidene fluoride membrane (Millipore) with a Trans‐Blot SD Semi‐Dry Electrophoretic Transfer Cell (Bio‐Rad).

    Article Title: Lactobacillus rhamnosus GR-1 Ameliorates Escherichia coli-Induced Inflammation and Cell Damage via Attenuation of ASC-Independent NLRP3 Inflammasome Activation
    Article Snippet: .. At 2, 4, and 6 h postinfection, BMECs were lysed in 1 ml of lysis buffer, composed of 1 ml of radioimmunoprecipitation assay buffer, 5 μl of protease inhibitor cocktail, and 5 μl of phenylmethanesulfonyl fluoride (Sigma-Aldrich). .. The resulting lysates were centrifuged at 13,000 × g for 10 min at 4°C to pellet insoluble material, and the supernatants were used for Western blot analysis.

    Protease Inhibitor:

    Article Title: Rpph1 Upregulates CDC42 Expression and Promotes Hippocampal Neuron Dendritic Spine Formation by Competing with miR-330-5p
    Article Snippet: .. Western Blotting Protein samples were lysed in RIPA buffer with a protease inhibitor cocktail (Sangon) and 1 mM phenylmethanesulfonyl fluoride (Sigma). ..

    Article Title: The Role of Na+/Ca2+ Exchanger 1 in Maintaining Ductus Arteriosus Patency
    Article Snippet: .. Protein extraction and Western blotting assay Human DA smooth muscle cells and mouse DA tissues were lysed in a RIPA buffer containing a protease inhibitor cocktail tablet (Roche Diagnostics, Indianapolis, USA) and Phenylmethanesulfonyl fluoride (Sigma). .. Twenty micrograms of total protein were electrophoresed on a 10% SDS-PAGE gel, transferred onto a nitrocellulose blotting membrane, blocked, and then incubated overnight with antibodies against NCX1(Sigma, USA) and GAPDH (Bioworld, China).

    Article Title: MiR-375 Regulation of LDHB Plays Distinct Roles in Polyomavirus-Positive and -Negative Merkel Cell Carcinoma
    Article Snippet: .. Cells were harvested and lysed using NP-40 lysis buffer (FNN0021; Life Technologies), supplemented with 1 mM of phenylmethanesulfonyl fluoride (PMSF, Sigma-Aldrich) and protease inhibitor (complete protease inhibitor cocktail; Roche Diagnostics GmbH). .. Protein concentrations were measured using the PierceTM BCA Protein assay kit (Thermo Fisher scientific, Inc., Waltham, MA, USA).

    Article Title: Lactobacillus rhamnosus GR-1 Ameliorates Escherichia coli-Induced Inflammation and Cell Damage via Attenuation of ASC-Independent NLRP3 Inflammasome Activation
    Article Snippet: .. At 2, 4, and 6 h postinfection, BMECs were lysed in 1 ml of lysis buffer, composed of 1 ml of radioimmunoprecipitation assay buffer, 5 μl of protease inhibitor cocktail, and 5 μl of phenylmethanesulfonyl fluoride (Sigma-Aldrich). .. The resulting lysates were centrifuged at 13,000 × g for 10 min at 4°C to pellet insoluble material, and the supernatants were used for Western blot analysis.

    Incubation:

    Article Title: An effector of a necrotrophic fungal pathogen targets the calcium‐sensing receptor in chloroplasts to inhibit host resistance, et al. An effector of a necrotrophic fungal pathogen targets the calcium sensing receptor in chloroplasts to inhibit host resistance
    Article Snippet: .. 4.3 Protein extraction, western blot, IP, and LC‐MS/MS assays For protein extraction, plant tissue was ground in liquid nitrogen and mixed with an equal volume of radio immunoprecipitation assay (RIPA) lysis buffer (Beyotime) with 1 mM phenylmethanesulfonyl fluoride and 1% proteinase inhibitor cocktail (Sigma), then incubated on ice for 30 min and centrifuged at 13,000 × g for 15 min at 4 °C. .. The supernatant was transferred to a new tube and boiled in sodium dodecyl sulphate (SDS) loading buffer for 5 min. Proteins were separated by SDS‐polyacrylamide gel electrophoresis (PAGE) (12%) followed by electroblotting onto a 0.22 μm polyvinylidene fluoride membrane (Millipore) with a Trans‐Blot SD Semi‐Dry Electrophoretic Transfer Cell (Bio‐Rad).

    Protein Extraction:

    Article Title: The Role of Na+/Ca2+ Exchanger 1 in Maintaining Ductus Arteriosus Patency
    Article Snippet: .. Protein extraction and Western blotting assay Human DA smooth muscle cells and mouse DA tissues were lysed in a RIPA buffer containing a protease inhibitor cocktail tablet (Roche Diagnostics, Indianapolis, USA) and Phenylmethanesulfonyl fluoride (Sigma). .. Twenty micrograms of total protein were electrophoresed on a 10% SDS-PAGE gel, transferred onto a nitrocellulose blotting membrane, blocked, and then incubated overnight with antibodies against NCX1(Sigma, USA) and GAPDH (Bioworld, China).

    Article Title: An effector of a necrotrophic fungal pathogen targets the calcium‐sensing receptor in chloroplasts to inhibit host resistance, et al. An effector of a necrotrophic fungal pathogen targets the calcium sensing receptor in chloroplasts to inhibit host resistance
    Article Snippet: .. 4.3 Protein extraction, western blot, IP, and LC‐MS/MS assays For protein extraction, plant tissue was ground in liquid nitrogen and mixed with an equal volume of radio immunoprecipitation assay (RIPA) lysis buffer (Beyotime) with 1 mM phenylmethanesulfonyl fluoride and 1% proteinase inhibitor cocktail (Sigma), then incubated on ice for 30 min and centrifuged at 13,000 × g for 15 min at 4 °C. .. The supernatant was transferred to a new tube and boiled in sodium dodecyl sulphate (SDS) loading buffer for 5 min. Proteins were separated by SDS‐polyacrylamide gel electrophoresis (PAGE) (12%) followed by electroblotting onto a 0.22 μm polyvinylidene fluoride membrane (Millipore) with a Trans‐Blot SD Semi‐Dry Electrophoretic Transfer Cell (Bio‐Rad).

    Western Blot:

    Article Title: Rpph1 Upregulates CDC42 Expression and Promotes Hippocampal Neuron Dendritic Spine Formation by Competing with miR-330-5p
    Article Snippet: .. Western Blotting Protein samples were lysed in RIPA buffer with a protease inhibitor cocktail (Sangon) and 1 mM phenylmethanesulfonyl fluoride (Sigma). ..

    Article Title: The Role of Na+/Ca2+ Exchanger 1 in Maintaining Ductus Arteriosus Patency
    Article Snippet: .. Protein extraction and Western blotting assay Human DA smooth muscle cells and mouse DA tissues were lysed in a RIPA buffer containing a protease inhibitor cocktail tablet (Roche Diagnostics, Indianapolis, USA) and Phenylmethanesulfonyl fluoride (Sigma). .. Twenty micrograms of total protein were electrophoresed on a 10% SDS-PAGE gel, transferred onto a nitrocellulose blotting membrane, blocked, and then incubated overnight with antibodies against NCX1(Sigma, USA) and GAPDH (Bioworld, China).

    Article Title: An effector of a necrotrophic fungal pathogen targets the calcium‐sensing receptor in chloroplasts to inhibit host resistance, et al. An effector of a necrotrophic fungal pathogen targets the calcium sensing receptor in chloroplasts to inhibit host resistance
    Article Snippet: .. 4.3 Protein extraction, western blot, IP, and LC‐MS/MS assays For protein extraction, plant tissue was ground in liquid nitrogen and mixed with an equal volume of radio immunoprecipitation assay (RIPA) lysis buffer (Beyotime) with 1 mM phenylmethanesulfonyl fluoride and 1% proteinase inhibitor cocktail (Sigma), then incubated on ice for 30 min and centrifuged at 13,000 × g for 15 min at 4 °C. .. The supernatant was transferred to a new tube and boiled in sodium dodecyl sulphate (SDS) loading buffer for 5 min. Proteins were separated by SDS‐polyacrylamide gel electrophoresis (PAGE) (12%) followed by electroblotting onto a 0.22 μm polyvinylidene fluoride membrane (Millipore) with a Trans‐Blot SD Semi‐Dry Electrophoretic Transfer Cell (Bio‐Rad).

    Lysis:

    Article Title: Phloretin exerts hypoglycemic effect in streptozotocin-induced diabetic rats and improves insulin resistance in vitro
    Article Snippet: .. Skeletal muscle tissue homogenates or total cells were lysed in RIPA lysis buffer (pH 8.0, 50 mmol/L Tris, 150 mmol/L NaCl, 0.02% sodium azide, 0.1% SDS, 1% NP-40, 0.5% sodium deoxycholate, 1 mmol/L EDTA) and soon supplemented with a phenylmethanesulfonyl fluoride (1 mmol/L; Sigma), protease, and phosphatase inhibitor cocktail (100×; Thermo Fisher Scientific, Waltham, MA, USA). .. Equal amounts of protein samples were separated by 10% (v/v) sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a polyvinylidene difluoride (PVDF) membrane.

    Article Title: MiR-375 Regulation of LDHB Plays Distinct Roles in Polyomavirus-Positive and -Negative Merkel Cell Carcinoma
    Article Snippet: .. Cells were harvested and lysed using NP-40 lysis buffer (FNN0021; Life Technologies), supplemented with 1 mM of phenylmethanesulfonyl fluoride (PMSF, Sigma-Aldrich) and protease inhibitor (complete protease inhibitor cocktail; Roche Diagnostics GmbH). .. Protein concentrations were measured using the PierceTM BCA Protein assay kit (Thermo Fisher scientific, Inc., Waltham, MA, USA).

    Article Title: An effector of a necrotrophic fungal pathogen targets the calcium‐sensing receptor in chloroplasts to inhibit host resistance, et al. An effector of a necrotrophic fungal pathogen targets the calcium sensing receptor in chloroplasts to inhibit host resistance
    Article Snippet: .. 4.3 Protein extraction, western blot, IP, and LC‐MS/MS assays For protein extraction, plant tissue was ground in liquid nitrogen and mixed with an equal volume of radio immunoprecipitation assay (RIPA) lysis buffer (Beyotime) with 1 mM phenylmethanesulfonyl fluoride and 1% proteinase inhibitor cocktail (Sigma), then incubated on ice for 30 min and centrifuged at 13,000 × g for 15 min at 4 °C. .. The supernatant was transferred to a new tube and boiled in sodium dodecyl sulphate (SDS) loading buffer for 5 min. Proteins were separated by SDS‐polyacrylamide gel electrophoresis (PAGE) (12%) followed by electroblotting onto a 0.22 μm polyvinylidene fluoride membrane (Millipore) with a Trans‐Blot SD Semi‐Dry Electrophoretic Transfer Cell (Bio‐Rad).

    Article Title: Lactobacillus rhamnosus GR-1 Ameliorates Escherichia coli-Induced Inflammation and Cell Damage via Attenuation of ASC-Independent NLRP3 Inflammasome Activation
    Article Snippet: .. At 2, 4, and 6 h postinfection, BMECs were lysed in 1 ml of lysis buffer, composed of 1 ml of radioimmunoprecipitation assay buffer, 5 μl of protease inhibitor cocktail, and 5 μl of phenylmethanesulfonyl fluoride (Sigma-Aldrich). .. The resulting lysates were centrifuged at 13,000 × g for 10 min at 4°C to pellet insoluble material, and the supernatants were used for Western blot analysis.

    Article Title: Preclinical studies reveal that LSD1 inhibition results in tumor growth arrest in lung adenocarcinoma independently of driver mutations
    Article Snippet: .. 2.4 Immunoblotting Cells were lysed by three freeze‐thaw cycles using cell lysis buffer (New England Biolabs Ipswich, MA, USA) supplemented with 1 mm phenylmethanesulfonyl fluoride (Sigma‐Aldrich). .. Subsequently, protein concentrations were determined using the Pierce™ BCA Protein Assay Kit (Thermo Fisher, Waltham, MA, USA).

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    Millipore phenylmethanesulfonyl fluoride
    Latent myostatin binds WFIKKN1 but not WFIKKN2. In Ni 2+ -affinity pull-down assays, 1 μ m latent myostatin was incubated for 1 h with 2 μ m His-tagged WFIKKN1 or 2 μ m His-tagged WFIKKN2 in NaCl/P i containing 50 m m imidazole, 0.1% Tween-20 and 100 μ m <t>phenylmethanesulfonyl</t> fluoride (pH 7.5), and the solutions were then mixed with 20 μL of Ni 2+ –nitrilotriacetic acid resin. After 15 min of agitation, the resin was washed with NaCl/P i , 50 m m imidazole, 0.5% Tween-20 and 100 μ m phenylmethanesulfonyl fluoride (pH 7.5), and the bound proteins were eluted with NaCl/P i and 500 m m imidazole (pH 7.5). The eluted samples were analyzed by SDS/PAGE, and the proteins were visualized by staining with Coomassie Brilliant Blue and by western blotting with specific antibodies against myostatin prodomain (anti-prodomain) and against mature myostatin (anti-myostatin). LM, latent myostatin. The numbers indicate the molecular mass values of proteins of the Low Molecular Weight Calibration Kit. In the upper panel, the proteins were visualized by staining with Coomassie Brilliant Blue; in the lower panels, the proteins were visualized by western blotting.
    Phenylmethanesulfonyl Fluoride, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenylmethanesulfonyl fluoride/product/Millipore
    Average 99 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    phenylmethanesulfonyl fluoride - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    85
    Millipore pmsf stock solution
    Latent myostatin binds WFIKKN1 but not WFIKKN2. In Ni 2+ -affinity pull-down assays, 1 μ m latent myostatin was incubated for 1 h with 2 μ m His-tagged WFIKKN1 or 2 μ m His-tagged WFIKKN2 in NaCl/P i containing 50 m m imidazole, 0.1% Tween-20 and 100 μ m <t>phenylmethanesulfonyl</t> fluoride (pH 7.5), and the solutions were then mixed with 20 μL of Ni 2+ –nitrilotriacetic acid resin. After 15 min of agitation, the resin was washed with NaCl/P i , 50 m m imidazole, 0.5% Tween-20 and 100 μ m phenylmethanesulfonyl fluoride (pH 7.5), and the bound proteins were eluted with NaCl/P i and 500 m m imidazole (pH 7.5). The eluted samples were analyzed by SDS/PAGE, and the proteins were visualized by staining with Coomassie Brilliant Blue and by western blotting with specific antibodies against myostatin prodomain (anti-prodomain) and against mature myostatin (anti-myostatin). LM, latent myostatin. The numbers indicate the molecular mass values of proteins of the Low Molecular Weight Calibration Kit. In the upper panel, the proteins were visualized by staining with Coomassie Brilliant Blue; in the lower panels, the proteins were visualized by western blotting.
    Pmsf Stock Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmsf stock solution/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pmsf stock solution - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Latent myostatin binds WFIKKN1 but not WFIKKN2. In Ni 2+ -affinity pull-down assays, 1 μ m latent myostatin was incubated for 1 h with 2 μ m His-tagged WFIKKN1 or 2 μ m His-tagged WFIKKN2 in NaCl/P i containing 50 m m imidazole, 0.1% Tween-20 and 100 μ m phenylmethanesulfonyl fluoride (pH 7.5), and the solutions were then mixed with 20 μL of Ni 2+ –nitrilotriacetic acid resin. After 15 min of agitation, the resin was washed with NaCl/P i , 50 m m imidazole, 0.5% Tween-20 and 100 μ m phenylmethanesulfonyl fluoride (pH 7.5), and the bound proteins were eluted with NaCl/P i and 500 m m imidazole (pH 7.5). The eluted samples were analyzed by SDS/PAGE, and the proteins were visualized by staining with Coomassie Brilliant Blue and by western blotting with specific antibodies against myostatin prodomain (anti-prodomain) and against mature myostatin (anti-myostatin). LM, latent myostatin. The numbers indicate the molecular mass values of proteins of the Low Molecular Weight Calibration Kit. In the upper panel, the proteins were visualized by staining with Coomassie Brilliant Blue; in the lower panels, the proteins were visualized by western blotting.

    Journal: The Febs Journal

    Article Title: Latent myostatin has significant activity and this activity is controlled more efficiently by WFIKKN1 than by WFIKKN2

    doi: 10.1111/febs.12377

    Figure Lengend Snippet: Latent myostatin binds WFIKKN1 but not WFIKKN2. In Ni 2+ -affinity pull-down assays, 1 μ m latent myostatin was incubated for 1 h with 2 μ m His-tagged WFIKKN1 or 2 μ m His-tagged WFIKKN2 in NaCl/P i containing 50 m m imidazole, 0.1% Tween-20 and 100 μ m phenylmethanesulfonyl fluoride (pH 7.5), and the solutions were then mixed with 20 μL of Ni 2+ –nitrilotriacetic acid resin. After 15 min of agitation, the resin was washed with NaCl/P i , 50 m m imidazole, 0.5% Tween-20 and 100 μ m phenylmethanesulfonyl fluoride (pH 7.5), and the bound proteins were eluted with NaCl/P i and 500 m m imidazole (pH 7.5). The eluted samples were analyzed by SDS/PAGE, and the proteins were visualized by staining with Coomassie Brilliant Blue and by western blotting with specific antibodies against myostatin prodomain (anti-prodomain) and against mature myostatin (anti-myostatin). LM, latent myostatin. The numbers indicate the molecular mass values of proteins of the Low Molecular Weight Calibration Kit. In the upper panel, the proteins were visualized by staining with Coomassie Brilliant Blue; in the lower panels, the proteins were visualized by western blotting.

    Article Snippet: Protein precipitates were removed by centrifugation at 3500 g for 15 min and the protein solution was dialyzed against 3 × 3 L of 100 mm Tris/HCl, 100 μm phenylmethanesulfonyl fluoride and 0.005% 2-mercaptoethanol (pH 8.0) at 4 °C for 36 h. Precipitates were removed by centrifugation at 3500 g for 15 min and the protein solution was then concentrated on an Amicon stirred ultrafiltration cell (EMD Millipore, Billerica, MA USA) and applied to a 20-mL Strep-Tactin Sepharose column.

    Techniques: Incubation, SDS Page, Staining, Western Blot, Molecular Weight