phenylmethanesulfonyl fluoride pmsf  (Beyotime)

 
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    Beyotime phenylmethanesulfonyl fluoride pmsf

    https://www.bioz.com/result/phenylmethanesulfonyl fluoride pmsf/product/Beyotime
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylmethanesulfonyl fluoride pmsf - by Bioz Stars, 2021-03
    97/100 stars

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    Related Articles

    Western Blot:

    Article Title: Ang-2 promotes lung cancer metastasis by increasing epithelial-mesenchymal transition
    Article Snippet: The expression and gene transcription of Ang-2 in human lung Beas-2B cells, lung cancer SPC-A-1, NCI-1650, A549, and NCI-1975 cell lines were analyzed at protein level by Western blotting and at mRNA level by quantitative real-time reverse transcription PCR (qRT-PCR). .. Western blotting Phenylmethyl sulfonyl fluoride (PMSF), Radio Immunoprecipitation Assay (RIPA) lysis buffer, and Bicinchoninic Acid (BCA) Protein Assay kit were obtained from the Beyotime Institute of Biotechnology, Shanghai, China. .. Cells were harvested and washed with cold PBS twice, then lysed on ice in RIPA lysis buffer (1×PBS, 1% NP-40, 0.1% sodium dodecylsulfate (SDS), 5 mM EDTA, 0.5% sodium deoxycholate, and 1 mM sodium orthovanadate) that contained 100 μg/mL PMSF and protease inhibitors (KeyGen, Nanjing, China).

    Article Title: LncRNA MALAT-1 promotes growth and metastasis of epithelial ovarian cancer via sponging microrna-22
    Article Snippet: .. Western blotsTotal protein was extracted from cell samples using RIPA Lysis Buffer (Beyotime, China) complemented with PMSF (Beyotime, China), followed by SDS-PAGE separation and electro-transfer onto PVDF membranes. .. After blocking the membranes using 5% skimmed milk, they were incubated with anti-c-myc (#18583, 1:1000 dilution), E-cadherin (#14472, 1:1000 dilution), Vimentin (#5741, 1:1000 dilution), and Snai1 (#3879, 1:1000 dilution) primary antibodies at 4°C overnight and then corresponding HRP-conjugated secondary antibodies (anti-rabbit, #14708, 1:2000 dilution; anti-mouse, #14708, 1:3000 dilution) at room temperature for 2 h. The protein expressions were visualized using ECL kit (Beyotime, China).

    Article Title: Inhibition of copper transporter 1 prevents α-synuclein pathology and alleviates nigrostriatal degeneration in AAV-based mouse model of Parkinson's disease
    Article Snippet: Yeast strains carrying the galactose-inducible Ctr1 constructs were pre-grown in SC medium with 1% raffinose and induced with 2% galactose. .. 2.9 Western blot analysisCell samples were rinsed three times with sterile PBS and were scraped into radioimmunoprecipitation assay lysis buffer (Beyotime) containing 2 mM PMSF (Beyotime) and 1% protease inhibitor cocktail (Sigma-Aldrich) incubated for 30 min at 4 °C. .. Mice tissues frozen and stored in a -80 °C refrigerator were fully immersed in the radioimmunoprecipitation assay lysis buffer containing 2 mM PMSF and 1% protease inhibitor cocktail, homogenated by ultrasonic crashing, and centrifuged at 15,000 rpm for 30 min at 4 °C.

    Article Title: Phytochemicals protect L02 cells against hepatotoxicity induced by emodin via the Nrf2 signaling pathway the Nrf2 signaling pathway †Electronic supplementary information (ESI) available. See DOI: 10.1039/c9tx00220k
    Article Snippet: .. Western blotting analysisA total of 500 μL of phenylmethylsulfonyl fluoride (PMSF)-containing radioimmunoprecipitation assay (RIPA) buffer (Beyotime, Hangzhou, CHINA) was added to the cells, and they were lysed on ice for 30 min. .. The protein concentrations were measured using a bicinchoninic acid (BCA) protein assay kit according to the manufacturer's instructions (Thermo Scientific, LA, USA).

    Radio Immunoprecipitation:

    Article Title: Ang-2 promotes lung cancer metastasis by increasing epithelial-mesenchymal transition
    Article Snippet: The expression and gene transcription of Ang-2 in human lung Beas-2B cells, lung cancer SPC-A-1, NCI-1650, A549, and NCI-1975 cell lines were analyzed at protein level by Western blotting and at mRNA level by quantitative real-time reverse transcription PCR (qRT-PCR). .. Western blotting Phenylmethyl sulfonyl fluoride (PMSF), Radio Immunoprecipitation Assay (RIPA) lysis buffer, and Bicinchoninic Acid (BCA) Protein Assay kit were obtained from the Beyotime Institute of Biotechnology, Shanghai, China. .. Cells were harvested and washed with cold PBS twice, then lysed on ice in RIPA lysis buffer (1×PBS, 1% NP-40, 0.1% sodium dodecylsulfate (SDS), 5 mM EDTA, 0.5% sodium deoxycholate, and 1 mM sodium orthovanadate) that contained 100 μg/mL PMSF and protease inhibitors (KeyGen, Nanjing, China).

    Article Title: Inhibition of copper transporter 1 prevents α-synuclein pathology and alleviates nigrostriatal degeneration in AAV-based mouse model of Parkinson's disease
    Article Snippet: Yeast strains carrying the galactose-inducible Ctr1 constructs were pre-grown in SC medium with 1% raffinose and induced with 2% galactose. .. 2.9 Western blot analysisCell samples were rinsed three times with sterile PBS and were scraped into radioimmunoprecipitation assay lysis buffer (Beyotime) containing 2 mM PMSF (Beyotime) and 1% protease inhibitor cocktail (Sigma-Aldrich) incubated for 30 min at 4 °C. .. Mice tissues frozen and stored in a -80 °C refrigerator were fully immersed in the radioimmunoprecipitation assay lysis buffer containing 2 mM PMSF and 1% protease inhibitor cocktail, homogenated by ultrasonic crashing, and centrifuged at 15,000 rpm for 30 min at 4 °C.

    Article Title: The Roles of Reactive Oxygen Species and Nitric Oxide in Perfluorooctanoic Acid-Induced Developmental Cardiotoxicity and l-Carnitine Mediated Protection
    Article Snippet: For p65, the cytoplasmic and nuclear protein extractions were prepared with the nuclear and cytoplasm protein extraction kit (KGP1100, KeyGenBioTECH, Nanjing, China) following the manufacturer provided protocol. .. For iNOS, independent samples were extracted with a radio immunoprecipitation assay buffer (RIPA) supplemented with 1:100 PMSF (Beyotime, Beijing, China). ..

    Article Title: Phytochemicals protect L02 cells against hepatotoxicity induced by emodin via the Nrf2 signaling pathway the Nrf2 signaling pathway †Electronic supplementary information (ESI) available. See DOI: 10.1039/c9tx00220k
    Article Snippet: .. Western blotting analysisA total of 500 μL of phenylmethylsulfonyl fluoride (PMSF)-containing radioimmunoprecipitation assay (RIPA) buffer (Beyotime, Hangzhou, CHINA) was added to the cells, and they were lysed on ice for 30 min. .. The protein concentrations were measured using a bicinchoninic acid (BCA) protein assay kit according to the manufacturer's instructions (Thermo Scientific, LA, USA).

    Article Title: A Simple System for Differentiation of Functional Intestinal Stem Cell-like Cells from Bone Marrow Mesenchymal Stem Cells
    Article Snippet: For miRNA analysis, rat U6 was used to normalize target miRNA expression. .. Cells were rinsed with PBS (pH 7.4) and lysed in radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime, Shanghai, China) with freshly added PMSF (Beyotime, Shanghai, China) for 20 min on ice. .. Sonication was used to facilitate cell lysis.

    Lysis:

    Article Title: Ang-2 promotes lung cancer metastasis by increasing epithelial-mesenchymal transition
    Article Snippet: The expression and gene transcription of Ang-2 in human lung Beas-2B cells, lung cancer SPC-A-1, NCI-1650, A549, and NCI-1975 cell lines were analyzed at protein level by Western blotting and at mRNA level by quantitative real-time reverse transcription PCR (qRT-PCR). .. Western blotting Phenylmethyl sulfonyl fluoride (PMSF), Radio Immunoprecipitation Assay (RIPA) lysis buffer, and Bicinchoninic Acid (BCA) Protein Assay kit were obtained from the Beyotime Institute of Biotechnology, Shanghai, China. .. Cells were harvested and washed with cold PBS twice, then lysed on ice in RIPA lysis buffer (1×PBS, 1% NP-40, 0.1% sodium dodecylsulfate (SDS), 5 mM EDTA, 0.5% sodium deoxycholate, and 1 mM sodium orthovanadate) that contained 100 μg/mL PMSF and protease inhibitors (KeyGen, Nanjing, China).

    Article Title: LncRNA MALAT-1 promotes growth and metastasis of epithelial ovarian cancer via sponging microrna-22
    Article Snippet: .. Western blotsTotal protein was extracted from cell samples using RIPA Lysis Buffer (Beyotime, China) complemented with PMSF (Beyotime, China), followed by SDS-PAGE separation and electro-transfer onto PVDF membranes. .. After blocking the membranes using 5% skimmed milk, they were incubated with anti-c-myc (#18583, 1:1000 dilution), E-cadherin (#14472, 1:1000 dilution), Vimentin (#5741, 1:1000 dilution), and Snai1 (#3879, 1:1000 dilution) primary antibodies at 4°C overnight and then corresponding HRP-conjugated secondary antibodies (anti-rabbit, #14708, 1:2000 dilution; anti-mouse, #14708, 1:3000 dilution) at room temperature for 2 h. The protein expressions were visualized using ECL kit (Beyotime, China).

    Article Title: Alteration of Androgen Receptor Protein Stability by Triptolide in LNCaP Cells
    Article Snippet: .. After co-treatment with triptolide and ALLM (50 μM and 100 μM), PMSF (100 μM) or BAPTA-AM (10 μM) for 24 h, the cells were lysed with RIPA lysis buffer (Beyotime Biotechnology, Haimen, Jiangsu, China) and the protein concentration was detected with BCA kit (Beyotime Biotechnology, Haimen, Jiangsu, China). ..

    Article Title: Inhibition of copper transporter 1 prevents α-synuclein pathology and alleviates nigrostriatal degeneration in AAV-based mouse model of Parkinson's disease
    Article Snippet: Yeast strains carrying the galactose-inducible Ctr1 constructs were pre-grown in SC medium with 1% raffinose and induced with 2% galactose. .. 2.9 Western blot analysisCell samples were rinsed three times with sterile PBS and were scraped into radioimmunoprecipitation assay lysis buffer (Beyotime) containing 2 mM PMSF (Beyotime) and 1% protease inhibitor cocktail (Sigma-Aldrich) incubated for 30 min at 4 °C. .. Mice tissues frozen and stored in a -80 °C refrigerator were fully immersed in the radioimmunoprecipitation assay lysis buffer containing 2 mM PMSF and 1% protease inhibitor cocktail, homogenated by ultrasonic crashing, and centrifuged at 15,000 rpm for 30 min at 4 °C.

    Article Title: Trans-Ferulic Acid-4-β-Glucoside Alleviates Cold-Induced Oxidative Stress and Promotes Cold Tolerance
    Article Snippet: .. The brown adipose tissue homogenate of mice was mixed with RIPA lysate (Beyotime Biotechnology, shanghai, China), protease inhibitor (Roche, Basel, Switzerland), and PMSF (Beyotime Biotechnology, shanghai, China) (100:4:1), and the mixture was placed on ice for 15 min to carry out cell lysis. .. Next, we measured protein concentration and added 5× loading buffer (Cwbiotech, Beijing, China), boiled the sample for 7 min, and loaded the proteins for electrophoresis.

    Article Title: A Simple System for Differentiation of Functional Intestinal Stem Cell-like Cells from Bone Marrow Mesenchymal Stem Cells
    Article Snippet: For miRNA analysis, rat U6 was used to normalize target miRNA expression. .. Cells were rinsed with PBS (pH 7.4) and lysed in radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime, Shanghai, China) with freshly added PMSF (Beyotime, Shanghai, China) for 20 min on ice. .. Sonication was used to facilitate cell lysis.

    BIA-KA:

    Article Title: Ang-2 promotes lung cancer metastasis by increasing epithelial-mesenchymal transition
    Article Snippet: The expression and gene transcription of Ang-2 in human lung Beas-2B cells, lung cancer SPC-A-1, NCI-1650, A549, and NCI-1975 cell lines were analyzed at protein level by Western blotting and at mRNA level by quantitative real-time reverse transcription PCR (qRT-PCR). .. Western blotting Phenylmethyl sulfonyl fluoride (PMSF), Radio Immunoprecipitation Assay (RIPA) lysis buffer, and Bicinchoninic Acid (BCA) Protein Assay kit were obtained from the Beyotime Institute of Biotechnology, Shanghai, China. .. Cells were harvested and washed with cold PBS twice, then lysed on ice in RIPA lysis buffer (1×PBS, 1% NP-40, 0.1% sodium dodecylsulfate (SDS), 5 mM EDTA, 0.5% sodium deoxycholate, and 1 mM sodium orthovanadate) that contained 100 μg/mL PMSF and protease inhibitors (KeyGen, Nanjing, China).

    Article Title: Alteration of Androgen Receptor Protein Stability by Triptolide in LNCaP Cells
    Article Snippet: .. After co-treatment with triptolide and ALLM (50 μM and 100 μM), PMSF (100 μM) or BAPTA-AM (10 μM) for 24 h, the cells were lysed with RIPA lysis buffer (Beyotime Biotechnology, Haimen, Jiangsu, China) and the protein concentration was detected with BCA kit (Beyotime Biotechnology, Haimen, Jiangsu, China). ..

    SDS Page:

    Article Title: LncRNA MALAT-1 promotes growth and metastasis of epithelial ovarian cancer via sponging microrna-22
    Article Snippet: .. Western blotsTotal protein was extracted from cell samples using RIPA Lysis Buffer (Beyotime, China) complemented with PMSF (Beyotime, China), followed by SDS-PAGE separation and electro-transfer onto PVDF membranes. .. After blocking the membranes using 5% skimmed milk, they were incubated with anti-c-myc (#18583, 1:1000 dilution), E-cadherin (#14472, 1:1000 dilution), Vimentin (#5741, 1:1000 dilution), and Snai1 (#3879, 1:1000 dilution) primary antibodies at 4°C overnight and then corresponding HRP-conjugated secondary antibodies (anti-rabbit, #14708, 1:2000 dilution; anti-mouse, #14708, 1:3000 dilution) at room temperature for 2 h. The protein expressions were visualized using ECL kit (Beyotime, China).

    Protein Concentration:

    Article Title: Alteration of Androgen Receptor Protein Stability by Triptolide in LNCaP Cells
    Article Snippet: .. After co-treatment with triptolide and ALLM (50 μM and 100 μM), PMSF (100 μM) or BAPTA-AM (10 μM) for 24 h, the cells were lysed with RIPA lysis buffer (Beyotime Biotechnology, Haimen, Jiangsu, China) and the protein concentration was detected with BCA kit (Beyotime Biotechnology, Haimen, Jiangsu, China). ..

    Protease Inhibitor:

    Article Title: Inhibition of copper transporter 1 prevents α-synuclein pathology and alleviates nigrostriatal degeneration in AAV-based mouse model of Parkinson's disease
    Article Snippet: Yeast strains carrying the galactose-inducible Ctr1 constructs were pre-grown in SC medium with 1% raffinose and induced with 2% galactose. .. 2.9 Western blot analysisCell samples were rinsed three times with sterile PBS and were scraped into radioimmunoprecipitation assay lysis buffer (Beyotime) containing 2 mM PMSF (Beyotime) and 1% protease inhibitor cocktail (Sigma-Aldrich) incubated for 30 min at 4 °C. .. Mice tissues frozen and stored in a -80 °C refrigerator were fully immersed in the radioimmunoprecipitation assay lysis buffer containing 2 mM PMSF and 1% protease inhibitor cocktail, homogenated by ultrasonic crashing, and centrifuged at 15,000 rpm for 30 min at 4 °C.

    Article Title: Trans-Ferulic Acid-4-β-Glucoside Alleviates Cold-Induced Oxidative Stress and Promotes Cold Tolerance
    Article Snippet: .. The brown adipose tissue homogenate of mice was mixed with RIPA lysate (Beyotime Biotechnology, shanghai, China), protease inhibitor (Roche, Basel, Switzerland), and PMSF (Beyotime Biotechnology, shanghai, China) (100:4:1), and the mixture was placed on ice for 15 min to carry out cell lysis. .. Next, we measured protein concentration and added 5× loading buffer (Cwbiotech, Beijing, China), boiled the sample for 7 min, and loaded the proteins for electrophoresis.

    Incubation:

    Article Title: Inhibition of copper transporter 1 prevents α-synuclein pathology and alleviates nigrostriatal degeneration in AAV-based mouse model of Parkinson's disease
    Article Snippet: Yeast strains carrying the galactose-inducible Ctr1 constructs were pre-grown in SC medium with 1% raffinose and induced with 2% galactose. .. 2.9 Western blot analysisCell samples were rinsed three times with sterile PBS and were scraped into radioimmunoprecipitation assay lysis buffer (Beyotime) containing 2 mM PMSF (Beyotime) and 1% protease inhibitor cocktail (Sigma-Aldrich) incubated for 30 min at 4 °C. .. Mice tissues frozen and stored in a -80 °C refrigerator were fully immersed in the radioimmunoprecipitation assay lysis buffer containing 2 mM PMSF and 1% protease inhibitor cocktail, homogenated by ultrasonic crashing, and centrifuged at 15,000 rpm for 30 min at 4 °C.

    Mouse Assay:

    Article Title: Trans-Ferulic Acid-4-β-Glucoside Alleviates Cold-Induced Oxidative Stress and Promotes Cold Tolerance
    Article Snippet: .. The brown adipose tissue homogenate of mice was mixed with RIPA lysate (Beyotime Biotechnology, shanghai, China), protease inhibitor (Roche, Basel, Switzerland), and PMSF (Beyotime Biotechnology, shanghai, China) (100:4:1), and the mixture was placed on ice for 15 min to carry out cell lysis. .. Next, we measured protein concentration and added 5× loading buffer (Cwbiotech, Beijing, China), boiled the sample for 7 min, and loaded the proteins for electrophoresis.

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  • 97
    Beyotime pmsf
    Western blotting for inducible nitric oxide synthase (iNOS) in ED19 chicken embryo hearts and hatchling chicken hearts. Fertile chicken eggs were air cell injected with vehicle, PFOA 2 mg/kg, or PFOA 2 mg/kg + l -carnitine 100 mg/kg, and then incubated to ED19 or hatch. At the desired stage, hearts were collected, protein was extracted with <t>RIPA</t> buffer supplemented with <t>PMSF</t> and then assessed with western blotting, and the expressions were normalized to control animals. ( A ) iNOS relative expression levels of ED19 chicken embryo hearts; ( B ) iNOS relative expression levels of hatchling chicken embryo hearts. N = 3 per group. * Statistically different from control group ( p
    Pmsf, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmsf/product/Beyotime
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pmsf - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    98
    Beyotime pmsf ripa
    Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% <t>PMSF-RIPA</t> and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P
    Pmsf Ripa, supplied by Beyotime, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmsf ripa/product/Beyotime
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pmsf ripa - by Bioz Stars, 2021-03
    98/100 stars
      Buy from Supplier

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    Western blotting for inducible nitric oxide synthase (iNOS) in ED19 chicken embryo hearts and hatchling chicken hearts. Fertile chicken eggs were air cell injected with vehicle, PFOA 2 mg/kg, or PFOA 2 mg/kg + l -carnitine 100 mg/kg, and then incubated to ED19 or hatch. At the desired stage, hearts were collected, protein was extracted with RIPA buffer supplemented with PMSF and then assessed with western blotting, and the expressions were normalized to control animals. ( A ) iNOS relative expression levels of ED19 chicken embryo hearts; ( B ) iNOS relative expression levels of hatchling chicken embryo hearts. N = 3 per group. * Statistically different from control group ( p

    Journal: International Journal of Molecular Sciences

    Article Title: The Roles of Reactive Oxygen Species and Nitric Oxide in Perfluorooctanoic Acid-Induced Developmental Cardiotoxicity and l-Carnitine Mediated Protection

    doi: 10.3390/ijms18061229

    Figure Lengend Snippet: Western blotting for inducible nitric oxide synthase (iNOS) in ED19 chicken embryo hearts and hatchling chicken hearts. Fertile chicken eggs were air cell injected with vehicle, PFOA 2 mg/kg, or PFOA 2 mg/kg + l -carnitine 100 mg/kg, and then incubated to ED19 or hatch. At the desired stage, hearts were collected, protein was extracted with RIPA buffer supplemented with PMSF and then assessed with western blotting, and the expressions were normalized to control animals. ( A ) iNOS relative expression levels of ED19 chicken embryo hearts; ( B ) iNOS relative expression levels of hatchling chicken embryo hearts. N = 3 per group. * Statistically different from control group ( p

    Article Snippet: For iNOS, independent samples were extracted with a radio immunoprecipitation assay buffer (RIPA) supplemented with 1:100 PMSF (Beyotime, Beijing, China).

    Techniques: Western Blot, Injection, Incubation, Expressing

    ALLM blocked the AR down-regulation in the presence of triptolide. ( A ) LNCaP cells were co-treated with triptolide (100 nM) and ALLM (100 μM) or PMSF (100 μM) for 24 h, the AR protein level was analyzed by western blot assay; ( B ) The relative density of AR (normalized with GAPDH in western blot assay) after LNCaP cells were co-treated with triptolide (100 nM) and ALLM (100 μM) or PMSF (100 μM) for 24 h; ( C ) The western bolt assay of AR protein levels in LNCaP cells after the cells were co-treated with triptolide (100 nM) and ALLM (50 μM); ( D ) The relative density of AR (normalized with triptolide) after the cells were co-treated with triptolide (100 nM) and ALLM (50 and 100 μM). (* p

    Journal: Medicina

    Article Title: Alteration of Androgen Receptor Protein Stability by Triptolide in LNCaP Cells

    doi: 10.3390/medicina54030039

    Figure Lengend Snippet: ALLM blocked the AR down-regulation in the presence of triptolide. ( A ) LNCaP cells were co-treated with triptolide (100 nM) and ALLM (100 μM) or PMSF (100 μM) for 24 h, the AR protein level was analyzed by western blot assay; ( B ) The relative density of AR (normalized with GAPDH in western blot assay) after LNCaP cells were co-treated with triptolide (100 nM) and ALLM (100 μM) or PMSF (100 μM) for 24 h; ( C ) The western bolt assay of AR protein levels in LNCaP cells after the cells were co-treated with triptolide (100 nM) and ALLM (50 μM); ( D ) The relative density of AR (normalized with triptolide) after the cells were co-treated with triptolide (100 nM) and ALLM (50 and 100 μM). (* p

    Article Snippet: After co-treatment with triptolide and ALLM (50 μM and 100 μM), PMSF (100 μM) or BAPTA-AM (10 μM) for 24 h, the cells were lysed with RIPA lysis buffer (Beyotime Biotechnology, Haimen, Jiangsu, China) and the protein concentration was detected with BCA kit (Beyotime Biotechnology, Haimen, Jiangsu, China).

    Techniques: Western Blot

    Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% PMSF-RIPA and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via Notch Signaling and Strengthens Dendritic Cell Mediated T Lymphocyte Cytotoxicity on Colon Cancer Cell CT26-WT

    doi: 10.1155/2018/2305683

    Figure Lengend Snippet: Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% PMSF-RIPA and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P

    Article Snippet: Western Blotting Assay Total protein product was extracted by incubating cells with 1% PMSF-RIPA (Catl. number ST506, Catl. number P0013B, Beyotime Biotechnology, China) and then centrifuged at 12000 rpm for 10 min. β -Actin was selected as the internal reference protein.

    Techniques: Incubation, Purification, RNA Extraction, Expressing, Polymerase Chain Reaction, Western Blot

    Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% PMSF-RIPA and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via Notch Signaling and Strengthens Dendritic Cell Mediated T Lymphocyte Cytotoxicity on Colon Cancer Cell CT26-WT

    doi: 10.1155/2018/2305683

    Figure Lengend Snippet: Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% PMSF-RIPA and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P

    Article Snippet: Total protein product was extracted by incubating cells with 1% PMSF-RIPA (Catl. number ST506, Catl. number P0013B, Beyotime Biotechnology, China) and then centrifuged at 12000 rpm for 10 min. β -Actin was selected as the internal reference protein.

    Techniques: Incubation, Purification, RNA Extraction, Expressing, Polymerase Chain Reaction, Western Blot