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Amresco phenol free total rna purification kit
Boxplot summary of all data . Semilogarithmic boxplot breakdown of <t>RNA</t> yield for <t>totalRNA,</t> smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and 2100 Bioanalyzer electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
Phenol Free Total Rna Purification Kit, supplied by Amresco, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phenol free total rna purification kit/product/Amresco
Average 88 stars, based on 2 article reviews
Price from $9.99 to $1999.99
phenol free total rna purification kit - by Bioz Stars, 2020-09
88/100 stars

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1) Product Images from "MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits"

Article Title: MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits

Journal: BMC Research Notes

doi: 10.1186/1756-0500-4-217

Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and 2100 Bioanalyzer electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
Figure Legend Snippet: Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and 2100 Bioanalyzer electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.

Techniques Used: Software, Electrophoresis, Standard Deviation

1 Gyrodactylus salaris individual . Electropherogram (Experion) of totalRNA extracted from 1 Gyrodactylus salaris individual using the Phenol-free Total RNA Purification kit from Amresco (AMR). 1% of the extraction was loaded on an Experion RNA HighSens Chip. In Gyrodactylus salaris the 28S RNA is probably autocatalytically degraded as described previously for insects [ 22 , 23 ]
Figure Legend Snippet: 1 Gyrodactylus salaris individual . Electropherogram (Experion) of totalRNA extracted from 1 Gyrodactylus salaris individual using the Phenol-free Total RNA Purification kit from Amresco (AMR). 1% of the extraction was loaded on an Experion RNA HighSens Chip. In Gyrodactylus salaris the 28S RNA is probably autocatalytically degraded as described previously for insects [ 22 , 23 ]

Techniques Used: Purification, Chromatin Immunoprecipitation

100 Gyrodactylus salaris individuals comparison . Electropherograms (Experion) of totalRNA preparations from 100 Gyrodactylus salaris individuals using the AMB, EPI and ZR kits. 1% of each extraction was loaded on a Experion RNA HighSens Chip. The Z-scale illustrates the RQI/RIN values of the respective preparations. The AMB protocol yields high quality RNA whereas the EPI extract contains a substantial proportion of degraded RNA. The ZR extract is of high quality although the RQI/RIN values are low due to the unusual ratio of smallRNA and 18S/28S RNA.
Figure Legend Snippet: 100 Gyrodactylus salaris individuals comparison . Electropherograms (Experion) of totalRNA preparations from 100 Gyrodactylus salaris individuals using the AMB, EPI and ZR kits. 1% of each extraction was loaded on a Experion RNA HighSens Chip. The Z-scale illustrates the RQI/RIN values of the respective preparations. The AMB protocol yields high quality RNA whereas the EPI extract contains a substantial proportion of degraded RNA. The ZR extract is of high quality although the RQI/RIN values are low due to the unusual ratio of smallRNA and 18S/28S RNA.

Techniques Used: Chromatin Immunoprecipitation

Salmo salar RNA . Electropherogram (Experion) of totalRNA extracted from uninfected fish-tissue prepared with AMR. 1% of the extraction was loaded on an Experion RNA HighSens Chip. The ranges of the 18S, 28S, and small RNA species are indicated.
Figure Legend Snippet: Salmo salar RNA . Electropherogram (Experion) of totalRNA extracted from uninfected fish-tissue prepared with AMR. 1% of the extraction was loaded on an Experion RNA HighSens Chip. The ranges of the 18S, 28S, and small RNA species are indicated.

Techniques Used: Fluorescence In Situ Hybridization, Chromatin Immunoprecipitation

2) Product Images from "Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis"

Article Title: Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis

Journal: BMC Research Notes

doi: 10.1186/s13104-015-1726-3

Electropherogram of RNA elutes obtained using three different RNA extraction methods. a RNA extracted from glucose-supplemented cells using TRI Reagent; b RNA extracted from PE-supplemented cells using TRI Reagent; c RNA extracted from glucose-supplemented cells using RNeasy kit; d RNA extracted from PE-supplemented cells using RNeasy kit; e RNA extracted from glucose-supplemented cells using Phenol-free kit; f RNA extracted from glucose-supplemented cells using Phenol-free kit. NA not available
Figure Legend Snippet: Electropherogram of RNA elutes obtained using three different RNA extraction methods. a RNA extracted from glucose-supplemented cells using TRI Reagent; b RNA extracted from PE-supplemented cells using TRI Reagent; c RNA extracted from glucose-supplemented cells using RNeasy kit; d RNA extracted from PE-supplemented cells using RNeasy kit; e RNA extracted from glucose-supplemented cells using Phenol-free kit; f RNA extracted from glucose-supplemented cells using Phenol-free kit. NA not available

Techniques Used: RNA Extraction

3) Product Images from "MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits"

Article Title: MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits

Journal: BMC Research Notes

doi: 10.1186/1756-0500-4-217

1 Gyrodactylus salaris individual . Electropherogram (Experion) of totalRNA extracted from 1 Gyrodactylus salaris individual using the Phenol-free Total RNA Purification kit from Amresco (AMR). 1% of the extraction was loaded on an Experion RNA HighSens Chip. In Gyrodactylus salaris the 28S RNA is probably autocatalytically degraded as described previously for insects [ 22 , 23 ]
Figure Legend Snippet: 1 Gyrodactylus salaris individual . Electropherogram (Experion) of totalRNA extracted from 1 Gyrodactylus salaris individual using the Phenol-free Total RNA Purification kit from Amresco (AMR). 1% of the extraction was loaded on an Experion RNA HighSens Chip. In Gyrodactylus salaris the 28S RNA is probably autocatalytically degraded as described previously for insects [ 22 , 23 ]

Techniques Used: Purification, Chromatin Immunoprecipitation

4) Product Images from "MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits"

Article Title: MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits

Journal: BMC Research Notes

doi: 10.1186/1756-0500-4-217

Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and 2100 Bioanalyzer electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
Figure Legend Snippet: Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and 2100 Bioanalyzer electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.

Techniques Used: Software, Electrophoresis, Standard Deviation

1 Gyrodactylus salaris individual . Electropherogram (Experion) of totalRNA extracted from 1 Gyrodactylus salaris individual using the Phenol-free Total RNA Purification kit from Amresco (AMR). 1% of the extraction was loaded on an Experion RNA HighSens Chip. In Gyrodactylus salaris the 28S RNA is probably autocatalytically degraded as described previously for insects [ 22 , 23 ]
Figure Legend Snippet: 1 Gyrodactylus salaris individual . Electropherogram (Experion) of totalRNA extracted from 1 Gyrodactylus salaris individual using the Phenol-free Total RNA Purification kit from Amresco (AMR). 1% of the extraction was loaded on an Experion RNA HighSens Chip. In Gyrodactylus salaris the 28S RNA is probably autocatalytically degraded as described previously for insects [ 22 , 23 ]

Techniques Used: Purification, Chromatin Immunoprecipitation

100 Gyrodactylus salaris individuals comparison . Electropherograms (Experion) of totalRNA preparations from 100 Gyrodactylus salaris individuals using the AMB, EPI and ZR kits. 1% of each extraction was loaded on a Experion RNA HighSens Chip. The Z-scale illustrates the RQI/RIN values of the respective preparations. The AMB protocol yields high quality RNA whereas the EPI extract contains a substantial proportion of degraded RNA. The ZR extract is of high quality although the RQI/RIN values are low due to the unusual ratio of smallRNA and 18S/28S RNA.
Figure Legend Snippet: 100 Gyrodactylus salaris individuals comparison . Electropherograms (Experion) of totalRNA preparations from 100 Gyrodactylus salaris individuals using the AMB, EPI and ZR kits. 1% of each extraction was loaded on a Experion RNA HighSens Chip. The Z-scale illustrates the RQI/RIN values of the respective preparations. The AMB protocol yields high quality RNA whereas the EPI extract contains a substantial proportion of degraded RNA. The ZR extract is of high quality although the RQI/RIN values are low due to the unusual ratio of smallRNA and 18S/28S RNA.

Techniques Used: Chromatin Immunoprecipitation

Salmo salar RNA . Electropherogram (Experion) of totalRNA extracted from uninfected fish-tissue prepared with AMR. 1% of the extraction was loaded on an Experion RNA HighSens Chip. The ranges of the 18S, 28S, and small RNA species are indicated.
Figure Legend Snippet: Salmo salar RNA . Electropherogram (Experion) of totalRNA extracted from uninfected fish-tissue prepared with AMR. 1% of the extraction was loaded on an Experion RNA HighSens Chip. The ranges of the 18S, 28S, and small RNA species are indicated.

Techniques Used: Fluorescence In Situ Hybridization, Chromatin Immunoprecipitation

5) Product Images from "Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis"

Article Title: Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis

Journal: BMC Research Notes

doi: 10.1186/s13104-015-1726-3

Electropherogram of RNA elutes obtained using three different RNA extraction methods. a RNA extracted from glucose-supplemented cells using TRI Reagent; b RNA extracted from PE-supplemented cells using TRI Reagent; c RNA extracted from glucose-supplemented cells using RNeasy kit; d RNA extracted from PE-supplemented cells using RNeasy kit; e RNA extracted from glucose-supplemented cells using Phenol-free kit; f RNA extracted from glucose-supplemented cells using Phenol-free kit. NA not available
Figure Legend Snippet: Electropherogram of RNA elutes obtained using three different RNA extraction methods. a RNA extracted from glucose-supplemented cells using TRI Reagent; b RNA extracted from PE-supplemented cells using TRI Reagent; c RNA extracted from glucose-supplemented cells using RNeasy kit; d RNA extracted from PE-supplemented cells using RNeasy kit; e RNA extracted from glucose-supplemented cells using Phenol-free kit; f RNA extracted from glucose-supplemented cells using Phenol-free kit. NA not available

Techniques Used: RNA Extraction

6) Product Images from "Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis"

Article Title: Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis

Journal: BMC Research Notes

doi: 10.1186/s13104-015-1726-3

Electropherogram of RNA elutes obtained using three different RNA extraction methods. a RNA extracted from glucose-supplemented cells using TRI Reagent; b RNA extracted from PE-supplemented cells using TRI Reagent; c RNA extracted from glucose-supplemented cells using RNeasy kit; d RNA extracted from PE-supplemented cells using RNeasy kit; e RNA extracted from glucose-supplemented cells using Phenol-free kit; f RNA extracted from glucose-supplemented cells using Phenol-free kit. NA not available
Figure Legend Snippet: Electropherogram of RNA elutes obtained using three different RNA extraction methods. a RNA extracted from glucose-supplemented cells using TRI Reagent; b RNA extracted from PE-supplemented cells using TRI Reagent; c RNA extracted from glucose-supplemented cells using RNeasy kit; d RNA extracted from PE-supplemented cells using RNeasy kit; e RNA extracted from glucose-supplemented cells using Phenol-free kit; f RNA extracted from glucose-supplemented cells using Phenol-free kit. NA not available

Techniques Used: RNA Extraction

7) Product Images from "MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits"

Article Title: MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits

Journal: BMC Research Notes

doi: 10.1186/1756-0500-4-217

Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and 2100 Bioanalyzer electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
Figure Legend Snippet: Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and 2100 Bioanalyzer electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.

Techniques Used: Software, Electrophoresis, Standard Deviation

1 Gyrodactylus salaris individual . Electropherogram (Experion) of totalRNA extracted from 1 Gyrodactylus salaris individual using the Phenol-free Total RNA Purification kit from Amresco (AMR). 1% of the extraction was loaded on an Experion RNA HighSens Chip. In Gyrodactylus salaris the 28S RNA is probably autocatalytically degraded as described previously for insects [ 22 , 23 ]
Figure Legend Snippet: 1 Gyrodactylus salaris individual . Electropherogram (Experion) of totalRNA extracted from 1 Gyrodactylus salaris individual using the Phenol-free Total RNA Purification kit from Amresco (AMR). 1% of the extraction was loaded on an Experion RNA HighSens Chip. In Gyrodactylus salaris the 28S RNA is probably autocatalytically degraded as described previously for insects [ 22 , 23 ]

Techniques Used: Purification, Chromatin Immunoprecipitation

100 Gyrodactylus salaris individuals comparison . Electropherograms (Experion) of totalRNA preparations from 100 Gyrodactylus salaris individuals using the AMB, EPI and ZR kits. 1% of each extraction was loaded on a Experion RNA HighSens Chip. The Z-scale illustrates the RQI/RIN values of the respective preparations. The AMB protocol yields high quality RNA whereas the EPI extract contains a substantial proportion of degraded RNA. The ZR extract is of high quality although the RQI/RIN values are low due to the unusual ratio of smallRNA and 18S/28S RNA.
Figure Legend Snippet: 100 Gyrodactylus salaris individuals comparison . Electropherograms (Experion) of totalRNA preparations from 100 Gyrodactylus salaris individuals using the AMB, EPI and ZR kits. 1% of each extraction was loaded on a Experion RNA HighSens Chip. The Z-scale illustrates the RQI/RIN values of the respective preparations. The AMB protocol yields high quality RNA whereas the EPI extract contains a substantial proportion of degraded RNA. The ZR extract is of high quality although the RQI/RIN values are low due to the unusual ratio of smallRNA and 18S/28S RNA.

Techniques Used: Chromatin Immunoprecipitation

Salmo salar RNA . Electropherogram (Experion) of totalRNA extracted from uninfected fish-tissue prepared with AMR. 1% of the extraction was loaded on an Experion RNA HighSens Chip. The ranges of the 18S, 28S, and small RNA species are indicated.
Figure Legend Snippet: Salmo salar RNA . Electropherogram (Experion) of totalRNA extracted from uninfected fish-tissue prepared with AMR. 1% of the extraction was loaded on an Experion RNA HighSens Chip. The ranges of the 18S, 28S, and small RNA species are indicated.

Techniques Used: Fluorescence In Situ Hybridization, Chromatin Immunoprecipitation

Related Articles

RNA Extraction:

Article Title: Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis
Article Snippet: .. RNA extraction RNA extraction was done with cell pellets containing 1 × 109 cells from three independent biological replicates of each condition using three different kits/reagent: TRI Reagent, RNeasy Mini kit and Phenol-free total RNA purification kit. .. TRI reagentTotal RNA extraction was performed according to the manufacturer’s protocol with slight modification.

Purification:

Article Title: MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits
Article Snippet: .. We evaluated 1 salt precipitation method (MasterPure™ Complete RNA Purification Kit, Epicentre), 2 Phenol based extraction methods (mirVana Kit, Ambion, and Trizol Plus Kit, Invitrogen), 1 paramagnetic bead extraction method (RNA Tissue kit, GeneMole) and 2 purification methods based on spin column chromatography using a proprietary resin as separation matrix (Phenol-free Total RNA Purification Kit, Amresco, and ZR MicroPrep Kit, Zymo Research). ..

Article Title: Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis
Article Snippet: .. With this in mind, we evaluated the performance of Phenol-free total RNA purification kit (Amresco) in comparison with TRI Reagent (MRC) and RNeasy Mini (Qiagen) for the extraction of total RNA of Pseudomonas aeruginosa which was grown in glucose-supplemented (control) and polyethylene-supplemented (growth-limiting condition) minimal medium. .. All three extraction methods were coupled with an in-house DNase I treatment before the yield, integrity and size distribution of the purified RNA were assessed.

Article Title: MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits
Article Snippet: .. Careful choice of totalRNA extraction kit is crucial, and in our comparison, the best results for totalRNA containing smallRNAs were obtained with the Phenol-free Total RNA Purification Kit (Amresco). .. However the highest amount of miRNAs with a yield of 9.6 ng for one individual could be extracted using the ZR RNA MicroPrep kit (Zymo Research), which is also the only kit that allows elution with a volume as little as 6 μl, one order of magnitude smaller than for the other kits and recommended as input for some downstream NGS applications (8 μl, ScriptMiner™ Small RNA-Seq Library Preparation Kit, Lit.

Article Title: MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits
Article Snippet: .. The Phenol-free Total RNA Purification Kit (Amresco) showed the highest totalRNA yield, but the best miRNA/totalRNA ratio was obtained with the ZR MicroPrep Kit (Zymo Research). .. It was not possible to extract electrophoretically detectable miRNAs from Gyrodactylus salaris with the RNA Tissue Kit (GeneMole) or the Trizol Plus Kit (Invitrogen).

Article Title: MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits
Article Snippet: .. Ribonucleic acid extraction Six commercially available kits were compared: MasterPure™ Complete RNA Purification Kit (Epicentre, EPI), Molestrips totalRNA basic for the GeneMole extraction robot (GeneMole, GM), Phenol-free Total RNA Purification Kit (Amresco, AMR), ZR RNA MicroPrep kit (Zymo Research, ZR), mirVana Kit (Ambion, AMB), and the Trizol Plus Kit (Invitrogen, INV). .. The selected kits represent four different extraction strategies for total RNA: rapid desalting and precipitation (EPI), solid phase extraction on silica-coated magnetic beads (GM), solid phase extraction on filter membranes (ZR, AMR) and sequential organic and solid phase extractions (AMB and INV).

Article Title: Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis
Article Snippet: .. RNA extraction RNA extraction was done with cell pellets containing 1 × 109 cells from three independent biological replicates of each condition using three different kits/reagent: TRI Reagent, RNeasy Mini kit and Phenol-free total RNA purification kit. .. TRI reagentTotal RNA extraction was performed according to the manufacturer’s protocol with slight modification.

Article Title: Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis
Article Snippet: .. RNeasy mini kit and phenol-free total RNA purification kit The cell pellet was reconstituted with 400 µl of sterile minimal media. .. Then, 800 µl RNAprotect Bacteria Reagent (Qiagen, USA) was added and the contents in the tube were vortexed prior to a 5 min incubation at room temperature (RT).

Article Title: Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis
Article Snippet: .. While Fromm et al. [ ] had shown that the Phenol-free total RNA purification kit (Amresco, USA) is capable of yielding low and high molecular weight transcripts from an ectoparasite, the suitability and performance of these RNA extracts were not evaluated using RNA-Seq. .. Determining the performance of Phenol-free RNA extracts in terms of transcriptome coverage and depth would especially benefit researchers who are interested in studying the expression of high and low abundance functional (mostly large mRNAs) and regulatory RNAs (often small RNAs) in a single sequencing run.

RNA Sequencing Assay:

Article Title: Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis
Article Snippet: .. While Fromm et al. [ ] had shown that the Phenol-free total RNA purification kit (Amresco, USA) is capable of yielding low and high molecular weight transcripts from an ectoparasite, the suitability and performance of these RNA extracts were not evaluated using RNA-Seq. .. Determining the performance of Phenol-free RNA extracts in terms of transcriptome coverage and depth would especially benefit researchers who are interested in studying the expression of high and low abundance functional (mostly large mRNAs) and regulatory RNAs (often small RNAs) in a single sequencing run.

Molecular Weight:

Article Title: Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis
Article Snippet: .. While Fromm et al. [ ] had shown that the Phenol-free total RNA purification kit (Amresco, USA) is capable of yielding low and high molecular weight transcripts from an ectoparasite, the suitability and performance of these RNA extracts were not evaluated using RNA-Seq. .. Determining the performance of Phenol-free RNA extracts in terms of transcriptome coverage and depth would especially benefit researchers who are interested in studying the expression of high and low abundance functional (mostly large mRNAs) and regulatory RNAs (often small RNAs) in a single sequencing run.

Column Chromatography:

Article Title: MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits
Article Snippet: .. We evaluated 1 salt precipitation method (MasterPure™ Complete RNA Purification Kit, Epicentre), 2 Phenol based extraction methods (mirVana Kit, Ambion, and Trizol Plus Kit, Invitrogen), 1 paramagnetic bead extraction method (RNA Tissue kit, GeneMole) and 2 purification methods based on spin column chromatography using a proprietary resin as separation matrix (Phenol-free Total RNA Purification Kit, Amresco, and ZR MicroPrep Kit, Zymo Research). ..

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    Amresco phenol free total rna purification kit
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of <t>RNA</t> yield for <t>totalRNA,</t> smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and 2100 Bioanalyzer electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    Phenol Free Total Rna Purification Kit, supplied by Amresco, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenol free total rna purification kit/product/Amresco
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    phenol free total rna purification kit - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

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    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and 2100 Bioanalyzer electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.

    Journal: BMC Research Notes

    Article Title: MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits

    doi: 10.1186/1756-0500-4-217

    Figure Lengend Snippet: Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and 2100 Bioanalyzer electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.

    Article Snippet: Careful choice of totalRNA extraction kit is crucial, and in our comparison, the best results for totalRNA containing smallRNAs were obtained with the Phenol-free Total RNA Purification Kit (Amresco).

    Techniques: Software, Electrophoresis, Standard Deviation

    1 Gyrodactylus salaris individual . Electropherogram (Experion) of totalRNA extracted from 1 Gyrodactylus salaris individual using the Phenol-free Total RNA Purification kit from Amresco (AMR). 1% of the extraction was loaded on an Experion RNA HighSens Chip. In Gyrodactylus salaris the 28S RNA is probably autocatalytically degraded as described previously for insects [ 22 , 23 ]

    Journal: BMC Research Notes

    Article Title: MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits

    doi: 10.1186/1756-0500-4-217

    Figure Lengend Snippet: 1 Gyrodactylus salaris individual . Electropherogram (Experion) of totalRNA extracted from 1 Gyrodactylus salaris individual using the Phenol-free Total RNA Purification kit from Amresco (AMR). 1% of the extraction was loaded on an Experion RNA HighSens Chip. In Gyrodactylus salaris the 28S RNA is probably autocatalytically degraded as described previously for insects [ 22 , 23 ]

    Article Snippet: Careful choice of totalRNA extraction kit is crucial, and in our comparison, the best results for totalRNA containing smallRNAs were obtained with the Phenol-free Total RNA Purification Kit (Amresco).

    Techniques: Purification, Chromatin Immunoprecipitation

    100 Gyrodactylus salaris individuals comparison . Electropherograms (Experion) of totalRNA preparations from 100 Gyrodactylus salaris individuals using the AMB, EPI and ZR kits. 1% of each extraction was loaded on a Experion RNA HighSens Chip. The Z-scale illustrates the RQI/RIN values of the respective preparations. The AMB protocol yields high quality RNA whereas the EPI extract contains a substantial proportion of degraded RNA. The ZR extract is of high quality although the RQI/RIN values are low due to the unusual ratio of smallRNA and 18S/28S RNA.

    Journal: BMC Research Notes

    Article Title: MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits

    doi: 10.1186/1756-0500-4-217

    Figure Lengend Snippet: 100 Gyrodactylus salaris individuals comparison . Electropherograms (Experion) of totalRNA preparations from 100 Gyrodactylus salaris individuals using the AMB, EPI and ZR kits. 1% of each extraction was loaded on a Experion RNA HighSens Chip. The Z-scale illustrates the RQI/RIN values of the respective preparations. The AMB protocol yields high quality RNA whereas the EPI extract contains a substantial proportion of degraded RNA. The ZR extract is of high quality although the RQI/RIN values are low due to the unusual ratio of smallRNA and 18S/28S RNA.

    Article Snippet: Careful choice of totalRNA extraction kit is crucial, and in our comparison, the best results for totalRNA containing smallRNAs were obtained with the Phenol-free Total RNA Purification Kit (Amresco).

    Techniques: Chromatin Immunoprecipitation

    Salmo salar RNA . Electropherogram (Experion) of totalRNA extracted from uninfected fish-tissue prepared with AMR. 1% of the extraction was loaded on an Experion RNA HighSens Chip. The ranges of the 18S, 28S, and small RNA species are indicated.

    Journal: BMC Research Notes

    Article Title: MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits

    doi: 10.1186/1756-0500-4-217

    Figure Lengend Snippet: Salmo salar RNA . Electropherogram (Experion) of totalRNA extracted from uninfected fish-tissue prepared with AMR. 1% of the extraction was loaded on an Experion RNA HighSens Chip. The ranges of the 18S, 28S, and small RNA species are indicated.

    Article Snippet: Careful choice of totalRNA extraction kit is crucial, and in our comparison, the best results for totalRNA containing smallRNAs were obtained with the Phenol-free Total RNA Purification Kit (Amresco).

    Techniques: Fluorescence In Situ Hybridization, Chromatin Immunoprecipitation

    Electropherogram of RNA elutes obtained using three different RNA extraction methods. a RNA extracted from glucose-supplemented cells using TRI Reagent; b RNA extracted from PE-supplemented cells using TRI Reagent; c RNA extracted from glucose-supplemented cells using RNeasy kit; d RNA extracted from PE-supplemented cells using RNeasy kit; e RNA extracted from glucose-supplemented cells using Phenol-free kit; f RNA extracted from glucose-supplemented cells using Phenol-free kit. NA not available

    Journal: BMC Research Notes

    Article Title: Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis

    doi: 10.1186/s13104-015-1726-3

    Figure Lengend Snippet: Electropherogram of RNA elutes obtained using three different RNA extraction methods. a RNA extracted from glucose-supplemented cells using TRI Reagent; b RNA extracted from PE-supplemented cells using TRI Reagent; c RNA extracted from glucose-supplemented cells using RNeasy kit; d RNA extracted from PE-supplemented cells using RNeasy kit; e RNA extracted from glucose-supplemented cells using Phenol-free kit; f RNA extracted from glucose-supplemented cells using Phenol-free kit. NA not available

    Article Snippet: RNeasy mini kit and phenol-free total RNA purification kit The cell pellet was reconstituted with 400 µl of sterile minimal media.

    Techniques: RNA Extraction