Journal: The American Journal of Pathology
Article Title: Association of B7-1 Co-Stimulation with the Development of Graft Arterial Disease
Figure Lengend Snippet: Representative panels and bar graphs showing the results of RPA. Ten micrograms of total RNA from each heart graft or normal heart was analyzed, using multiprobe RPA kit mCK-1 (containing DNA templates for IL-4, IL-5, IL-10, IL-13, IL-15, IL-9, IL-2, IL-6, IFN-γ, L32, and GAPDH). A: Representative gel images analyzed by mCK-1. B: Bar graph showing the results of densitometric analysis for IL-4 gene expression in the allografts ( n = 3 for each group). IL-4 gene expression decreased significantly in the allografts in B7-2 −/− and B7-1/B7-2 −/− recipients compared with wild-type recipients. C: Bar graph showing the results of densitometric analysis for IFN-γ gene expression in the allografts ( n = 3 for each group). IFN-γ gene expression was comparable in all allograft groups.
Article Snippet: Purified anti-CD45R/B220 antibody (RA3-6B2), purified anti-CD11b antibody (M1/70), purified anti-CD16/CD32 antibody (Fc-block; clone 2.4G2), purified anti-CD28 antibody (37.51), purified anti-CD4 antibody (RM4-5), purified anti-CD8 antibody (53–6.7), fluorescein isothiocyanate (FITC)-conjugated anti-CD11b antibody (M1/70), FITC-conjugated anti-I-Ab antibody (25-9-17), FITC-conjugated isotype-matched IgG controls, biotinylated anti-B7-1 antibody (16-10A1), biotinylated anti-B7-2 antibody (GL1), biotinylated anti-CD40 antibody (3/23), biotinylated anti-CD40 ligand antibody (MR1), biotinylated anti-I-Ab antibody (25-9-17), biotinylated isotype-matched IgG controls, phycoerythrin-conjugated streptavidin, and the Riboquant multiprobe RPA kit (mCK-1 and mCK-5) were obtained from PharMingen (San Diego, CA).
Techniques: Recombinase Polymerase Amplification, Expressing