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U73122 PLC pharmacological blockers u73122
Involvement of TRPC 5 in Ca 2+ influx. NG 108‐15 cells were transfected with TRPC 5‐specific sh RNA or control sh RNA for 48 hr. Suppression of TRPC 5 was demonstrated with RT ‐ PCR (a), immunoblotting (b), and immunocytochemical staining (c). (d) [Ca 2+ ] i measurement showing inhibition of galectin‐1 (Gal‐1)‐induced [Ca 2+ ] i elevation by TRPC 5‐specific sh RNA but not control sh RNA . (e) Cells were treated with the phospholipase C ( PLC ) inhibitor <t>U73122</t> (1 μM), the PI (3)K inhibitors LY 294002 (5 μM) and wortmannin (2 μM), showing inhibition of Gal‐1‐induced Ca 2+ response.
Pharmacological Blockers U73122, supplied by U73122 PLC, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pharmacological blockers u73122 - by Bioz Stars, 2020-11
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gal‐1‐induced neuritogenesis

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1) Product Images from "Functional interplay between ganglioside GM1 and cross‐linking galectin‐1 induces axon‐like neuritogenesis via integrin‐based signaling and TRPC5‐dependent Ca2+ influx"

Article Title: Functional interplay between ganglioside GM1 and cross‐linking galectin‐1 induces axon‐like neuritogenesis via integrin‐based signaling and TRPC5‐dependent Ca2+ influx

Journal: Journal of Neurochemistry

doi: 10.1111/jnc.13418

Involvement of TRPC 5 in Ca 2+ influx. NG 108‐15 cells were transfected with TRPC 5‐specific sh RNA or control sh RNA for 48 hr. Suppression of TRPC 5 was demonstrated with RT ‐ PCR (a), immunoblotting (b), and immunocytochemical staining (c). (d) [Ca 2+ ] i measurement showing inhibition of galectin‐1 (Gal‐1)‐induced [Ca 2+ ] i elevation by TRPC 5‐specific sh RNA but not control sh RNA . (e) Cells were treated with the phospholipase C ( PLC ) inhibitor U73122 (1 μM), the PI (3)K inhibitors LY 294002 (5 μM) and wortmannin (2 μM), showing inhibition of Gal‐1‐induced Ca 2+ response.
Figure Legend Snippet: Involvement of TRPC 5 in Ca 2+ influx. NG 108‐15 cells were transfected with TRPC 5‐specific sh RNA or control sh RNA for 48 hr. Suppression of TRPC 5 was demonstrated with RT ‐ PCR (a), immunoblotting (b), and immunocytochemical staining (c). (d) [Ca 2+ ] i measurement showing inhibition of galectin‐1 (Gal‐1)‐induced [Ca 2+ ] i elevation by TRPC 5‐specific sh RNA but not control sh RNA . (e) Cells were treated with the phospholipase C ( PLC ) inhibitor U73122 (1 μM), the PI (3)K inhibitors LY 294002 (5 μM) and wortmannin (2 μM), showing inhibition of Gal‐1‐induced Ca 2+ response.

Techniques Used: Transfection, Reverse Transcription Polymerase Chain Reaction, Staining, Inhibition, Planar Chromatography

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Article Title: Functional interplay between ganglioside GM1 and cross‐linking galectin‐1 induces axon‐like neuritogenesis via integrin‐based signaling and TRPC5‐dependent Ca2+ influx
Article Snippet: .. For testing involvement of PLC, PI(3)K and TRPC5 channels in Gal‐1‐induced neuritogenesis, pharmacological blockers U73122 (PLC, 1–5 μM), wortmannin (PI(3)K, 1–5 μM), LY294002 (PI(3)K, 1–5 μM) and SK & F 96365 (TRP channel, 10–50 μM) were co‐applied. ..

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    U73122 PLC pharmacological blockers u73122
    Involvement of TRPC 5 in Ca 2+ influx. NG 108‐15 cells were transfected with TRPC 5‐specific sh RNA or control sh RNA for 48 hr. Suppression of TRPC 5 was demonstrated with RT ‐ PCR (a), immunoblotting (b), and immunocytochemical staining (c). (d) [Ca 2+ ] i measurement showing inhibition of galectin‐1 (Gal‐1)‐induced [Ca 2+ ] i elevation by TRPC 5‐specific sh RNA but not control sh RNA . (e) Cells were treated with the phospholipase C ( PLC ) inhibitor <t>U73122</t> (1 μM), the PI (3)K inhibitors LY 294002 (5 μM) and wortmannin (2 μM), showing inhibition of Gal‐1‐induced Ca 2+ response.
    Pharmacological Blockers U73122, supplied by U73122 PLC, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pharmacological blockers u73122/product/U73122 PLC
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pharmacological blockers u73122 - by Bioz Stars, 2020-11
    88/100 stars
      Buy from Supplier

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    Involvement of TRPC 5 in Ca 2+ influx. NG 108‐15 cells were transfected with TRPC 5‐specific sh RNA or control sh RNA for 48 hr. Suppression of TRPC 5 was demonstrated with RT ‐ PCR (a), immunoblotting (b), and immunocytochemical staining (c). (d) [Ca 2+ ] i measurement showing inhibition of galectin‐1 (Gal‐1)‐induced [Ca 2+ ] i elevation by TRPC 5‐specific sh RNA but not control sh RNA . (e) Cells were treated with the phospholipase C ( PLC ) inhibitor U73122 (1 μM), the PI (3)K inhibitors LY 294002 (5 μM) and wortmannin (2 μM), showing inhibition of Gal‐1‐induced Ca 2+ response.

    Journal: Journal of Neurochemistry

    Article Title: Functional interplay between ganglioside GM1 and cross‐linking galectin‐1 induces axon‐like neuritogenesis via integrin‐based signaling and TRPC5‐dependent Ca2+ influx

    doi: 10.1111/jnc.13418

    Figure Lengend Snippet: Involvement of TRPC 5 in Ca 2+ influx. NG 108‐15 cells were transfected with TRPC 5‐specific sh RNA or control sh RNA for 48 hr. Suppression of TRPC 5 was demonstrated with RT ‐ PCR (a), immunoblotting (b), and immunocytochemical staining (c). (d) [Ca 2+ ] i measurement showing inhibition of galectin‐1 (Gal‐1)‐induced [Ca 2+ ] i elevation by TRPC 5‐specific sh RNA but not control sh RNA . (e) Cells were treated with the phospholipase C ( PLC ) inhibitor U73122 (1 μM), the PI (3)K inhibitors LY 294002 (5 μM) and wortmannin (2 μM), showing inhibition of Gal‐1‐induced Ca 2+ response.

    Article Snippet: For testing involvement of PLC, PI(3)K and TRPC5 channels in Gal‐1‐induced neuritogenesis, pharmacological blockers U73122 (PLC, 1–5 μM), wortmannin (PI(3)K, 1–5 μM), LY294002 (PI(3)K, 1–5 μM) and SK & F 96365 (TRP channel, 10–50 μM) were co‐applied.

    Techniques: Transfection, Reverse Transcription Polymerase Chain Reaction, Staining, Inhibition, Planar Chromatography