pha  (Vector Laboratories)


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    Structured Review

    Vector Laboratories pha
    Pha, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pha/product/Vector Laboratories
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pha - by Bioz Stars, 2022-07
    95/100 stars

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  • 86
    Vector Laboratories pha e
    Lectin histochemical staining profiles in sections of OA cartilage. (a) Binding of PNA to complex chondrons of a severely degenerated cartilage region could be completely blocked with lactose (inset) ascertaining carbohydrate-specific binding. (b) Omission of the incubation step with biotinylated LEA (first-step reagent) from processing excluded probe-independent signal generation. (c, d) ConA staining: staining pattern of MS ≤4 regions included chondrocytes in deep zones of cartilage (c) . Intense staining of matrix and chondrons (inset) in MS ≥9 regions (d) . (e) PSA staining: positivity of chondrons (inset) and matrix, predominantly in MS ≥9 cartilage. (f, g) <t>PHA-E</t> staining: whereas MS ≤4 regions were negative (f) , MS ≥9 areas (g) presented positive chondrons (inset) and matrix. (h) PHA-L staining: binding sites were restricted to chondrons (insert) and matrix of MS ≥9 cartilage. (i-j) VAA staining: whereas the chondrons of MS ≤4 areas were negative (i) , reactivity was observed both in chondrons (inset) and matrix of MS ≥9 cartilage (j) . (k) LEA staining: reactivity for chondrons (inset) and matrix of MS ≥9 regions. (l) MAA-I staining: reactivity included chondrons (inset) and matrix of MS ≥9 cartilage. (m, n) SNA staining: weak staining of matrix and no staining of chondrons in MS ≤4 cartilage (m) , whereas both chondrons (inset) and matrix were positive in MS ≥9 regions (n) . (o) <t>DBA</t> staining: positivity in chondrons (inset) and matrix of MS ≥9 cartilage. (p, q) PNA staining: positive chondrocytes sparely distributed in the deeper zones of MS ≤4 cartilage ( p ; arrows, inset). In MS ≥9 cartilage (q) , intense matrix staining was observed, whereas chondrons were mostly negative (inset). (r) JAC staining: absent in chondrons (inset), but present in superficial zones of MS ≥9 cartilage. Bars in inserts of d,e,g,l,n,p,q,r: 50μm. Bars in inserts of h , j , k , o : 100μm. MS, Mankin score; OA, osteoarthritis.
    Pha E, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pha e/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pha e - by Bioz Stars, 2022-07
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    93
    Vector Laboratories fluorescein labeled l pha
    CTD110.6 recognizes both O- GlcNAc and terminal β-GlcNAc on cell surface glycoproteins. A , N- ), of swainsonine. B , binding of <t>L-PHA</t> ( upper ) and mAb CTD110.6 ( lower ) to fixed cells. Lec2 and Lec8 cells were cultured with or without 5 μg/ml swainsonine ( SW ) for 4 days. Cells (4 × 10 5 ) were incubated with 20 μg/ml fluorescein-labeled L-PHA or 40 μg/ml CTD110.6 on ice for 20 min followed by 10 μg/ml Cy5-conjugated anti-mouse IgM antibody. Gray profiles , secondary Abs; thin line , control cells; bold line , swainsonine-treated cells. Representative results are from two independent experiments.
    Fluorescein Labeled L Pha, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Vector Laboratories phaseolus vulgaris leucoagglutinin
    High glucose-stimulated UT-A1 sialylation is blocked by a PKC inhibitor. (A and B) UT-A1-MDCK cells were preincubated with 5.5 mM glucose medium for 24 hours and then incubated with DMEM containing 5.5 or 25 mM glucose or 25 mM L-glucose for another 24 hours. Cells were lysed in RIPA buffer, equal amounts of total cell lysates were used for GNL and SNA lectin pull-down assay, and UT-A1 protein expression was analyzed by Western blot. (A) Total or (B) lectin-precipitated UT-A1 was examined by Western blot. (C) UT-A1 MDCK cells were treated as above, except for that one group had a 2-hour pretreatment with 10 µ M chelerythrine (Chel). The bar graph shows band densities as fold of control (means±SDs; n =3). Ctrl, control; DSL, datura stramonium lectin; IB, immunoblot; PHA, <t>phaseolus</t> <t>vulgaris</t> <t>leucoagglutinin;</t> Tomato, lycopersicum esculentum lectin; WGA, wheat germ agglutinin. * P
    Phaseolus Vulgaris Leucoagglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phaseolus vulgaris leucoagglutinin/product/Vector Laboratories
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    92
    Vector Laboratories biotinylated pha l
    Loss of MGAT4A activity does not affect GlcNAc-β-1,6 branching. A , biosynthetic pathway of tetraantennary branched N -glycans. Binding sites of <t>PHA-L</t> are indicated. B , analysis of PHA-L binding to the Consortium of Functional Glycomics ( CFG ) glycan array v. 5.0 indicates a preference for GlcNAc-β-1,6 branched products. The graph shows the data for 100 μg/ml <t>biotinylated-PHA-L</t> (Vector Laboratories) binding to glycans bearing only β-1,4 branching (β -(1,4) ) and the corresponding β-1,6 glycans (β -(1,6) ). Data are given as fluorescence intensity. C , knockdown of MGAT4A in MCF-7 cells shows no significant change in MGAT4B or MGAT5 mRNA levels by qRT-PCR. The graph shows -fold change mRNA expression compared with NTC. n = 3 biological replicates. Error bars represent the S.D. D , fluorescence microscopy of PHA-L stained MGAT4A-KD and NTC cells. No significant loss of PHA-L binding were observed. Images shown are representative of two biological replicates, five images per replicate.
    Biotinylated Pha L, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated pha l/product/Vector Laboratories
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Lectin histochemical staining profiles in sections of OA cartilage. (a) Binding of PNA to complex chondrons of a severely degenerated cartilage region could be completely blocked with lactose (inset) ascertaining carbohydrate-specific binding. (b) Omission of the incubation step with biotinylated LEA (first-step reagent) from processing excluded probe-independent signal generation. (c, d) ConA staining: staining pattern of MS ≤4 regions included chondrocytes in deep zones of cartilage (c) . Intense staining of matrix and chondrons (inset) in MS ≥9 regions (d) . (e) PSA staining: positivity of chondrons (inset) and matrix, predominantly in MS ≥9 cartilage. (f, g) PHA-E staining: whereas MS ≤4 regions were negative (f) , MS ≥9 areas (g) presented positive chondrons (inset) and matrix. (h) PHA-L staining: binding sites were restricted to chondrons (insert) and matrix of MS ≥9 cartilage. (i-j) VAA staining: whereas the chondrons of MS ≤4 areas were negative (i) , reactivity was observed both in chondrons (inset) and matrix of MS ≥9 cartilage (j) . (k) LEA staining: reactivity for chondrons (inset) and matrix of MS ≥9 regions. (l) MAA-I staining: reactivity included chondrons (inset) and matrix of MS ≥9 cartilage. (m, n) SNA staining: weak staining of matrix and no staining of chondrons in MS ≤4 cartilage (m) , whereas both chondrons (inset) and matrix were positive in MS ≥9 regions (n) . (o) DBA staining: positivity in chondrons (inset) and matrix of MS ≥9 cartilage. (p, q) PNA staining: positive chondrocytes sparely distributed in the deeper zones of MS ≤4 cartilage ( p ; arrows, inset). In MS ≥9 cartilage (q) , intense matrix staining was observed, whereas chondrons were mostly negative (inset). (r) JAC staining: absent in chondrons (inset), but present in superficial zones of MS ≥9 cartilage. Bars in inserts of d,e,g,l,n,p,q,r: 50μm. Bars in inserts of h , j , k , o : 100μm. MS, Mankin score; OA, osteoarthritis.

    Journal: Arthritis Research & Therapy

    Article Title: Glycophenotyping of osteoarthritic cartilage and chondrocytes by RT-qPCR, mass spectrometry, histochemistry with plant/human lectins and lectin localization with a glycoprotein

    doi: 10.1186/ar4330

    Figure Lengend Snippet: Lectin histochemical staining profiles in sections of OA cartilage. (a) Binding of PNA to complex chondrons of a severely degenerated cartilage region could be completely blocked with lactose (inset) ascertaining carbohydrate-specific binding. (b) Omission of the incubation step with biotinylated LEA (first-step reagent) from processing excluded probe-independent signal generation. (c, d) ConA staining: staining pattern of MS ≤4 regions included chondrocytes in deep zones of cartilage (c) . Intense staining of matrix and chondrons (inset) in MS ≥9 regions (d) . (e) PSA staining: positivity of chondrons (inset) and matrix, predominantly in MS ≥9 cartilage. (f, g) PHA-E staining: whereas MS ≤4 regions were negative (f) , MS ≥9 areas (g) presented positive chondrons (inset) and matrix. (h) PHA-L staining: binding sites were restricted to chondrons (insert) and matrix of MS ≥9 cartilage. (i-j) VAA staining: whereas the chondrons of MS ≤4 areas were negative (i) , reactivity was observed both in chondrons (inset) and matrix of MS ≥9 cartilage (j) . (k) LEA staining: reactivity for chondrons (inset) and matrix of MS ≥9 regions. (l) MAA-I staining: reactivity included chondrons (inset) and matrix of MS ≥9 cartilage. (m, n) SNA staining: weak staining of matrix and no staining of chondrons in MS ≤4 cartilage (m) , whereas both chondrons (inset) and matrix were positive in MS ≥9 regions (n) . (o) DBA staining: positivity in chondrons (inset) and matrix of MS ≥9 cartilage. (p, q) PNA staining: positive chondrocytes sparely distributed in the deeper zones of MS ≤4 cartilage ( p ; arrows, inset). In MS ≥9 cartilage (q) , intense matrix staining was observed, whereas chondrons were mostly negative (inset). (r) JAC staining: absent in chondrons (inset), but present in superficial zones of MS ≥9 cartilage. Bars in inserts of d,e,g,l,n,p,q,r: 50μm. Bars in inserts of h , j , k , o : 100μm. MS, Mankin score; OA, osteoarthritis.

    Article Snippet: The plant lectins DBA, JAC, LEA, MAA-I, PHA-E, PHA-L, PNA and SNA were obtained as biotinylated probes from Vector Labs Burlingame, CA, USA.

    Techniques: Staining, Binding Assay, Incubation, Mass Spectrometry

    CTD110.6 recognizes both O- GlcNAc and terminal β-GlcNAc on cell surface glycoproteins. A , N- ), of swainsonine. B , binding of L-PHA ( upper ) and mAb CTD110.6 ( lower ) to fixed cells. Lec2 and Lec8 cells were cultured with or without 5 μg/ml swainsonine ( SW ) for 4 days. Cells (4 × 10 5 ) were incubated with 20 μg/ml fluorescein-labeled L-PHA or 40 μg/ml CTD110.6 on ice for 20 min followed by 10 μg/ml Cy5-conjugated anti-mouse IgM antibody. Gray profiles , secondary Abs; thin line , control cells; bold line , swainsonine-treated cells. Representative results are from two independent experiments.

    Journal: The Journal of Biological Chemistry

    Article Title: Antibodies That Detect O-Linked β-d-N-Acetylglucosamine on the Extracellular Domain of Cell Surface Glycoproteins *

    doi: 10.1074/jbc.M113.492512

    Figure Lengend Snippet: CTD110.6 recognizes both O- GlcNAc and terminal β-GlcNAc on cell surface glycoproteins. A , N- ), of swainsonine. B , binding of L-PHA ( upper ) and mAb CTD110.6 ( lower ) to fixed cells. Lec2 and Lec8 cells were cultured with or without 5 μg/ml swainsonine ( SW ) for 4 days. Cells (4 × 10 5 ) were incubated with 20 μg/ml fluorescein-labeled L-PHA or 40 μg/ml CTD110.6 on ice for 20 min followed by 10 μg/ml Cy5-conjugated anti-mouse IgM antibody. Gray profiles , secondary Abs; thin line , control cells; bold line , swainsonine-treated cells. Representative results are from two independent experiments.

    Article Snippet: Washed cells were incubated with 50 μl of binding buffer containing 1 μg of fluorescein-labeled L-PHA ( Phaseolus vulgaris leukoagglutinin; Vector, Burlingame, CA) or 2 μg of CTD110.6 mAb, followed by incubation with 50 μl of binding buffer containing 0.5 μg of Cy5-conjugated anti-mouse IgM.

    Techniques: Binding Assay, Cell Culture, Incubation, Labeling

    High glucose-stimulated UT-A1 sialylation is blocked by a PKC inhibitor. (A and B) UT-A1-MDCK cells were preincubated with 5.5 mM glucose medium for 24 hours and then incubated with DMEM containing 5.5 or 25 mM glucose or 25 mM L-glucose for another 24 hours. Cells were lysed in RIPA buffer, equal amounts of total cell lysates were used for GNL and SNA lectin pull-down assay, and UT-A1 protein expression was analyzed by Western blot. (A) Total or (B) lectin-precipitated UT-A1 was examined by Western blot. (C) UT-A1 MDCK cells were treated as above, except for that one group had a 2-hour pretreatment with 10 µ M chelerythrine (Chel). The bar graph shows band densities as fold of control (means±SDs; n =3). Ctrl, control; DSL, datura stramonium lectin; IB, immunoblot; PHA, phaseolus vulgaris leucoagglutinin; Tomato, lycopersicum esculentum lectin; WGA, wheat germ agglutinin. * P

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: Activation of Protein Kinase C-α and Src Kinase Increases Urea Transporter A1 α-2, 6 Sialylation

    doi: 10.1681/ASN.2014010026

    Figure Lengend Snippet: High glucose-stimulated UT-A1 sialylation is blocked by a PKC inhibitor. (A and B) UT-A1-MDCK cells were preincubated with 5.5 mM glucose medium for 24 hours and then incubated with DMEM containing 5.5 or 25 mM glucose or 25 mM L-glucose for another 24 hours. Cells were lysed in RIPA buffer, equal amounts of total cell lysates were used for GNL and SNA lectin pull-down assay, and UT-A1 protein expression was analyzed by Western blot. (A) Total or (B) lectin-precipitated UT-A1 was examined by Western blot. (C) UT-A1 MDCK cells were treated as above, except for that one group had a 2-hour pretreatment with 10 µ M chelerythrine (Chel). The bar graph shows band densities as fold of control (means±SDs; n =3). Ctrl, control; DSL, datura stramonium lectin; IB, immunoblot; PHA, phaseolus vulgaris leucoagglutinin; Tomato, lycopersicum esculentum lectin; WGA, wheat germ agglutinin. * P

    Article Snippet: Agarose-bound concanavalin A, wheat germ agglutinin, SNA, GNL, datura stramonium lectin, phaseolus vulgaris leucoagglutinin, and Tomato lectin (Lycopersicum esculentum lectin) were purchased from Vector Laboratories (Burlingame, CA).

    Techniques: Incubation, Pull Down Assay, Expressing, Western Blot, Whole Genome Amplification

    PKC activator increases UT-A1 glycan sialylation. (A) UT-A1 MDCK cells were treated with 2 µ M PDBu for 24 hours. The cells were lysed in RIPA buffer, equal amounts of total lysates were incubated with agarose-conjugated lectins, and then, they were immunoblotted with antibody to UT-A1. (B) Kidney IMCD suspensions prepared from Sprague–Dawley rats were treated with 2 µ M PDBu for 6 hours. The cell membrane lipid rafts were prepared by a 5%–40% sucrose gradient ultracentrifugation. Fractions 2–5 were collected for lectin pull-down assay with different lectins. The lectin-precipitated samples were then analyzed for UT-A1 protein expression by Western blot. The bar graph shows band densities as fold of control (means±SDs; n =3). Con A, concanavalin A; Ctrl, control; DSL, datura stramonium lectin; IB, immunoblot; PHA, phaseolus vulgaris leucoagglutinin; Tomato, lycopersicum esculentum lectin; WGA, wheat germ agglutinin. ** P

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: Activation of Protein Kinase C-α and Src Kinase Increases Urea Transporter A1 α-2, 6 Sialylation

    doi: 10.1681/ASN.2014010026

    Figure Lengend Snippet: PKC activator increases UT-A1 glycan sialylation. (A) UT-A1 MDCK cells were treated with 2 µ M PDBu for 24 hours. The cells were lysed in RIPA buffer, equal amounts of total lysates were incubated with agarose-conjugated lectins, and then, they were immunoblotted with antibody to UT-A1. (B) Kidney IMCD suspensions prepared from Sprague–Dawley rats were treated with 2 µ M PDBu for 6 hours. The cell membrane lipid rafts were prepared by a 5%–40% sucrose gradient ultracentrifugation. Fractions 2–5 were collected for lectin pull-down assay with different lectins. The lectin-precipitated samples were then analyzed for UT-A1 protein expression by Western blot. The bar graph shows band densities as fold of control (means±SDs; n =3). Con A, concanavalin A; Ctrl, control; DSL, datura stramonium lectin; IB, immunoblot; PHA, phaseolus vulgaris leucoagglutinin; Tomato, lycopersicum esculentum lectin; WGA, wheat germ agglutinin. ** P

    Article Snippet: Agarose-bound concanavalin A, wheat germ agglutinin, SNA, GNL, datura stramonium lectin, phaseolus vulgaris leucoagglutinin, and Tomato lectin (Lycopersicum esculentum lectin) were purchased from Vector Laboratories (Burlingame, CA).

    Techniques: Incubation, Pull Down Assay, Expressing, Western Blot, Whole Genome Amplification

    Loss of MGAT4A activity does not affect GlcNAc-β-1,6 branching. A , biosynthetic pathway of tetraantennary branched N -glycans. Binding sites of PHA-L are indicated. B , analysis of PHA-L binding to the Consortium of Functional Glycomics ( CFG ) glycan array v. 5.0 indicates a preference for GlcNAc-β-1,6 branched products. The graph shows the data for 100 μg/ml biotinylated-PHA-L (Vector Laboratories) binding to glycans bearing only β-1,4 branching (β -(1,4) ) and the corresponding β-1,6 glycans (β -(1,6) ). Data are given as fluorescence intensity. C , knockdown of MGAT4A in MCF-7 cells shows no significant change in MGAT4B or MGAT5 mRNA levels by qRT-PCR. The graph shows -fold change mRNA expression compared with NTC. n = 3 biological replicates. Error bars represent the S.D. D , fluorescence microscopy of PHA-L stained MGAT4A-KD and NTC cells. No significant loss of PHA-L binding were observed. Images shown are representative of two biological replicates, five images per replicate.

    Journal: The Journal of Biological Chemistry

    Article Title: MicroRNA-424 Predicts a Role for β-1,4 Branched Glycosylation in Cell Cycle Progression *

    doi: 10.1074/jbc.M115.672220

    Figure Lengend Snippet: Loss of MGAT4A activity does not affect GlcNAc-β-1,6 branching. A , biosynthetic pathway of tetraantennary branched N -glycans. Binding sites of PHA-L are indicated. B , analysis of PHA-L binding to the Consortium of Functional Glycomics ( CFG ) glycan array v. 5.0 indicates a preference for GlcNAc-β-1,6 branched products. The graph shows the data for 100 μg/ml biotinylated-PHA-L (Vector Laboratories) binding to glycans bearing only β-1,4 branching (β -(1,4) ) and the corresponding β-1,6 glycans (β -(1,6) ). Data are given as fluorescence intensity. C , knockdown of MGAT4A in MCF-7 cells shows no significant change in MGAT4B or MGAT5 mRNA levels by qRT-PCR. The graph shows -fold change mRNA expression compared with NTC. n = 3 biological replicates. Error bars represent the S.D. D , fluorescence microscopy of PHA-L stained MGAT4A-KD and NTC cells. No significant loss of PHA-L binding were observed. Images shown are representative of two biological replicates, five images per replicate.

    Article Snippet: Samples were then blocked with 5% bovine serum albumin in HBSS (2 ml, 30 min) and incubated with primary antibodies or lectins diluted in HBSS as follows for 1 h at room temperature: α-galectin-3 (1:250), α-E-cadherin (1:500), biotinylated-PHA-L (1:500, Vector Laboratories).

    Techniques: Activity Assay, Binding Assay, Functional Assay, Plasmid Preparation, Fluorescence, Quantitative RT-PCR, Expressing, Microscopy, Staining