pgl2 basic pgl2b luciferase reporter gene vector  (Promega)

 
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    Structured Review

    Promega pgl2 basic pgl2b luciferase reporter gene vector
    ESE-1 decreases COL2A1 promoter activity and enhances IL-1β-dependent inhibition. The C-28/I2 chondrocytes were cotransfected with <t>pGL2B</t> or with <t>pGL2-COL2</t> together with pCI empty vector or with (A) increasing amounts of pCI-ESE1 (50, 100, and
    Pgl2 Basic Pgl2b Luciferase Reporter Gene Vector, supplied by Promega, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgl2 basic pgl2b luciferase reporter gene vector/product/Promega
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pgl2 basic pgl2b luciferase reporter gene vector - by Bioz Stars, 2020-04
    87/100 stars

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    Images

    1) Product Images from "ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes"

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes

    Journal:

    doi: 10.1002/jcp.21338

    ESE-1 decreases COL2A1 promoter activity and enhances IL-1β-dependent inhibition. The C-28/I2 chondrocytes were cotransfected with pGL2B or with pGL2-COL2 together with pCI empty vector or with (A) increasing amounts of pCI-ESE1 (50, 100, and
    Figure Legend Snippet: ESE-1 decreases COL2A1 promoter activity and enhances IL-1β-dependent inhibition. The C-28/I2 chondrocytes were cotransfected with pGL2B or with pGL2-COL2 together with pCI empty vector or with (A) increasing amounts of pCI-ESE1 (50, 100, and

    Techniques Used: Activity Assay, Inhibition, Plasmid Preparation

    Related Articles

    Clone Assay:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: .. The COL2A1 sequence spanning –577 to +3,428 bp ( ; ) was cloned into the pGL2-basic (pGL2B) luciferase reporter gene vector (Promega) to generate the pGL2-COL2 construct containing approximately 4 kb of 5′-flanking sequence. .. Mutation of the translation start site ATG to CTG in the pGL2-COL2 construct did not increase the activity indicating that splicing occurred correctly and did not interfere with translation of the luciferase reporter.

    Luciferase:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: .. The COL2A1 sequence spanning –577 to +3,428 bp ( ; ) was cloned into the pGL2-basic (pGL2B) luciferase reporter gene vector (Promega) to generate the pGL2-COL2 construct containing approximately 4 kb of 5′-flanking sequence. .. Mutation of the translation start site ATG to CTG in the pGL2-COL2 construct did not increase the activity indicating that splicing occurred correctly and did not interfere with translation of the luciferase reporter.

    Ligation:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: The COL2A1 sequence spanning –577 to +3,428 bp ( ; ) was cloned into the pGL2-basic (pGL2B) luciferase reporter gene vector (Promega) to generate the pGL2-COL2 construct containing approximately 4 kb of 5′-flanking sequence. .. Deletion constructs containing –577/+125 bp, –403/+125 bp, –131/+125 bp, –90/+125 bp were prepared by restriction enzyme digestion of pGL2-COL2 at PstI, NheI, NcoI, ApaI, and PvuII sites, respectively, followed by ligation.

    Mutagenesis:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: The COL2A1 sequence spanning –577 to +3,428 bp ( ; ) was cloned into the pGL2-basic (pGL2B) luciferase reporter gene vector (Promega) to generate the pGL2-COL2 construct containing approximately 4 kb of 5′-flanking sequence. .. Mutation of the translation start site ATG to CTG in the pGL2-COL2 construct did not increase the activity indicating that splicing occurred correctly and did not interfere with translation of the luciferase reporter.

    Construct:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: .. The COL2A1 sequence spanning –577 to +3,428 bp ( ; ) was cloned into the pGL2-basic (pGL2B) luciferase reporter gene vector (Promega) to generate the pGL2-COL2 construct containing approximately 4 kb of 5′-flanking sequence. .. Mutation of the translation start site ATG to CTG in the pGL2-COL2 construct did not increase the activity indicating that splicing occurred correctly and did not interfere with translation of the luciferase reporter.

    Sequencing:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: .. The COL2A1 sequence spanning –577 to +3,428 bp ( ; ) was cloned into the pGL2-basic (pGL2B) luciferase reporter gene vector (Promega) to generate the pGL2-COL2 construct containing approximately 4 kb of 5′-flanking sequence. .. Mutation of the translation start site ATG to CTG in the pGL2-COL2 construct did not increase the activity indicating that splicing occurred correctly and did not interfere with translation of the luciferase reporter.

    Polymerase Chain Reaction:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: The COL2A1 sequence spanning –577 to +3,428 bp ( ; ) was cloned into the pGL2-basic (pGL2B) luciferase reporter gene vector (Promega) to generate the pGL2-COL2 construct containing approximately 4 kb of 5′-flanking sequence. .. Mutations in ETS sites in the pGL2-COL2 construct were generated using the PCR-based QuikChange™ Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA).

    Generated:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: The COL2A1 sequence spanning –577 to +3,428 bp ( ; ) was cloned into the pGL2-basic (pGL2B) luciferase reporter gene vector (Promega) to generate the pGL2-COL2 construct containing approximately 4 kb of 5′-flanking sequence. .. Mutations in ETS sites in the pGL2-COL2 construct were generated using the PCR-based QuikChange™ Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA).

    Activity Assay:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: The COL2A1 sequence spanning –577 to +3,428 bp ( ; ) was cloned into the pGL2-basic (pGL2B) luciferase reporter gene vector (Promega) to generate the pGL2-COL2 construct containing approximately 4 kb of 5′-flanking sequence. .. Mutation of the translation start site ATG to CTG in the pGL2-COL2 construct did not increase the activity indicating that splicing occurred correctly and did not interfere with translation of the luciferase reporter.

    Expressing:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: Paragraph title: Luciferase reporter constructs and expression plasmids ... The COL2A1 sequence spanning –577 to +3,428 bp ( ; ) was cloned into the pGL2-basic (pGL2B) luciferase reporter gene vector (Promega) to generate the pGL2-COL2 construct containing approximately 4 kb of 5′-flanking sequence.

    CTG Assay:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: The COL2A1 sequence spanning –577 to +3,428 bp ( ; ) was cloned into the pGL2-basic (pGL2B) luciferase reporter gene vector (Promega) to generate the pGL2-COL2 construct containing approximately 4 kb of 5′-flanking sequence. .. Mutation of the translation start site ATG to CTG in the pGL2-COL2 construct did not increase the activity indicating that splicing occurred correctly and did not interfere with translation of the luciferase reporter.

    Plasmid Preparation:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: .. The COL2A1 sequence spanning –577 to +3,428 bp ( ; ) was cloned into the pGL2-basic (pGL2B) luciferase reporter gene vector (Promega) to generate the pGL2-COL2 construct containing approximately 4 kb of 5′-flanking sequence. .. Mutation of the translation start site ATG to CTG in the pGL2-COL2 construct did not increase the activity indicating that splicing occurred correctly and did not interfere with translation of the luciferase reporter.

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  • News
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  • Team
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  • Contact
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  • 87
    Promega pgl2 basic pgl2b luciferase reporter gene vector
    ESE-1 decreases COL2A1 promoter activity and enhances IL-1β-dependent inhibition. The C-28/I2 chondrocytes were cotransfected with <t>pGL2B</t> or with <t>pGL2-COL2</t> together with pCI empty vector or with (A) increasing amounts of pCI-ESE1 (50, 100, and
    Pgl2 Basic Pgl2b Luciferase Reporter Gene Vector, supplied by Promega, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgl2 basic pgl2b luciferase reporter gene vector/product/Promega
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pgl2 basic pgl2b luciferase reporter gene vector - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

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    ESE-1 decreases COL2A1 promoter activity and enhances IL-1β-dependent inhibition. The C-28/I2 chondrocytes were cotransfected with pGL2B or with pGL2-COL2 together with pCI empty vector or with (A) increasing amounts of pCI-ESE1 (50, 100, and

    Journal:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes

    doi: 10.1002/jcp.21338

    Figure Lengend Snippet: ESE-1 decreases COL2A1 promoter activity and enhances IL-1β-dependent inhibition. The C-28/I2 chondrocytes were cotransfected with pGL2B or with pGL2-COL2 together with pCI empty vector or with (A) increasing amounts of pCI-ESE1 (50, 100, and

    Article Snippet: The COL2A1 sequence spanning –577 to +3,428 bp ( ; ) was cloned into the pGL2-basic (pGL2B) luciferase reporter gene vector (Promega) to generate the pGL2-COL2 construct containing approximately 4 kb of 5′-flanking sequence.

    Techniques: Activity Assay, Inhibition, Plasmid Preparation