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pgl 3- basic vector  (Promega)

 
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    Structured Review

    Promega pgl 3- basic vector
    Pgl 3 Basic Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl+3+basic+vector/pgl3+basic/pm38360124-80-27-31
    Average 90 stars, based on 1 article reviews
    pgl 3- basic vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: PCP4/PEP19 upregulates aromatase gene expression via CYP19A1 promoter I.1 in human breast cancer SK-BR-3 cells
    Article Snippet: .. The pGL 3 basic vector (Promega, Maddison, WI) was used as a control for luciferase assay. .. For knockdown experiments, pre-designed siRNAs were used for knockdown of PCP4/PEP19 (ID: HSS181928), ESR1 (ID: VHS40913) and Stealth RNAi siRNA Negative Control was used as negative control (Thermo Fisher Scientific, Waltham, MA).

    Article Title: LINC00174 is an oncogenic lncRNA of hepatocellular carcinoma and regulates miR-320/S100A10 axis.
    Article Snippet: Department of Second Gastroenterology, The First Affiliated Hospital of Jiamusi University, Jiamusi, China Department of Urology, The First Affiliated Hospital of Jiamusi University, Jiamusi, China Department of Vascular Surgery, The First Affiliated Hospital of Jiamusi University, Jiamusi, China Intensive Care Unit, The First Affiliated Hospital of Jiamusi University, Jiamusi, China Department of Blood Transfusion, Affiliated Hospital of Xiangnan University, Chenzhou, China

    Control:

    Article Title: PCP4/PEP19 upregulates aromatase gene expression via CYP19A1 promoter I.1 in human breast cancer SK-BR-3 cells
    Article Snippet: .. The pGL 3 basic vector (Promega, Maddison, WI) was used as a control for luciferase assay. .. For knockdown experiments, pre-designed siRNAs were used for knockdown of PCP4/PEP19 (ID: HSS181928), ESR1 (ID: VHS40913) and Stealth RNAi siRNA Negative Control was used as negative control (Thermo Fisher Scientific, Waltham, MA).

    Luciferase:

    Article Title: PCP4/PEP19 upregulates aromatase gene expression via CYP19A1 promoter I.1 in human breast cancer SK-BR-3 cells
    Article Snippet: .. The pGL 3 basic vector (Promega, Maddison, WI) was used as a control for luciferase assay. .. For knockdown experiments, pre-designed siRNAs were used for knockdown of PCP4/PEP19 (ID: HSS181928), ESR1 (ID: VHS40913) and Stealth RNAi siRNA Negative Control was used as negative control (Thermo Fisher Scientific, Waltham, MA).

    Clone Assay:

    Article Title: New alternative promoter in regulation of the oct-1 human gene transcription
    Article Snippet: For the first time, the presence of a new alternative promoter in the gene of the oct 1 transcription factor from which a previously unknown mRNA isoform Oct 1X, with 5' terminus different from the previ ously described isoforms, was demonstrated.. The nucleotide sequence of the Oct 1X cDNA was determined and the presence of a long open reading frame which starts with the first ATG codon of the second exon was demonstrated.. A protein shortened on its N terminus is assumed to be the product of a new mRNA isoform.

    Mutagenesis:

    Article Title: LINC00174 is an oncogenic lncRNA of hepatocellular carcinoma and regulates miR-320/S100A10 axis.
    Article Snippet: Department of Second Gastroenterology, The First Affiliated Hospital of Jiamusi University, Jiamusi, China Department of Urology, The First Affiliated Hospital of Jiamusi University, Jiamusi, China Department of Vascular Surgery, The First Affiliated Hospital of Jiamusi University, Jiamusi, China Intensive Care Unit, The First Affiliated Hospital of Jiamusi University, Jiamusi, China Department of Blood Transfusion, Affiliated Hospital of Xiangnan University, Chenzhou, China

    Sequencing:

    Article Title: LINC00174 is an oncogenic lncRNA of hepatocellular carcinoma and regulates miR-320/S100A10 axis.
    Article Snippet: Department of Second Gastroenterology, The First Affiliated Hospital of Jiamusi University, Jiamusi, China Department of Urology, The First Affiliated Hospital of Jiamusi University, Jiamusi, China Department of Vascular Surgery, The First Affiliated Hospital of Jiamusi University, Jiamusi, China Intensive Care Unit, The First Affiliated Hospital of Jiamusi University, Jiamusi, China Department of Blood Transfusion, Affiliated Hospital of Xiangnan University, Chenzhou, China

    Transfection:

    Article Title: LINC00174 is an oncogenic lncRNA of hepatocellular carcinoma and regulates miR-320/S100A10 axis.
    Article Snippet: Department of Second Gastroenterology, The First Affiliated Hospital of Jiamusi University, Jiamusi, China Department of Urology, The First Affiliated Hospital of Jiamusi University, Jiamusi, China Department of Vascular Surgery, The First Affiliated Hospital of Jiamusi University, Jiamusi, China Intensive Care Unit, The First Affiliated Hospital of Jiamusi University, Jiamusi, China Department of Blood Transfusion, Affiliated Hospital of Xiangnan University, Chenzhou, China

    Negative Control:

    Article Title: LINC00174 is an oncogenic lncRNA of hepatocellular carcinoma and regulates miR-320/S100A10 axis.
    Article Snippet: Department of Second Gastroenterology, The First Affiliated Hospital of Jiamusi University, Jiamusi, China Department of Urology, The First Affiliated Hospital of Jiamusi University, Jiamusi, China Department of Vascular Surgery, The First Affiliated Hospital of Jiamusi University, Jiamusi, China Intensive Care Unit, The First Affiliated Hospital of Jiamusi University, Jiamusi, China Department of Blood Transfusion, Affiliated Hospital of Xiangnan University, Chenzhou, China



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    Image Search Results


    The (−2436/+149) region of the PAK3 promoter, containing several putative cJun/AP- binding sites, was cloned into the pGL 3 -Basic reporter vector. A: Luciferase promoter reporter assays performed in control cells, Rat1a-GFP, and Rat1a-J4 cells transiently transfected with empty vector, pGL 3 -Basic, and PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). Cells were grown in the absence and presence of doxycycline. B: Luciferase promoter reporter assays performed in the parental rat fibroblast cell line, Rat1a, transfected with either pCMV-cJun or empty pCMV vector with the PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). A plasmid containing four AP-1 binding sites, 4 X AP-1-Luc, was used as a control showing cJun activation. C: Luciferase reporter promoter assays for deletion constructs of the PAK3 promoter region (−2436/+149), (−2329/+149), (−684/+149) and (−179/+149) as well as a (−179/+149) with a mutated cJun/AP-1 binding site at position (+52/+60), in the presence and absence of doxycycline-induced cJun/AP-1 expression. D: Luciferase reporter assays, using the (−179/+149) PAK3 promoter construct, showing the effect of transient cJun inhibition on PAK3 promoter activity in the absence and presence of doxycycline induced cJun/AP-1 over-expression. Results show the mean±S.E. of experiments performed in triplicate and repeated at least three times. (*p≤0.05 and **p≤0.01).

    Journal: PLoS ONE

    Article Title: p21-Activated Kinase 3 (PAK3) Is an AP-1 Regulated Gene Contributing to Actin Organisation and Migration of Transformed Fibroblasts

    doi: 10.1371/journal.pone.0066892

    Figure Lengend Snippet: The (−2436/+149) region of the PAK3 promoter, containing several putative cJun/AP- binding sites, was cloned into the pGL 3 -Basic reporter vector. A: Luciferase promoter reporter assays performed in control cells, Rat1a-GFP, and Rat1a-J4 cells transiently transfected with empty vector, pGL 3 -Basic, and PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). Cells were grown in the absence and presence of doxycycline. B: Luciferase promoter reporter assays performed in the parental rat fibroblast cell line, Rat1a, transfected with either pCMV-cJun or empty pCMV vector with the PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). A plasmid containing four AP-1 binding sites, 4 X AP-1-Luc, was used as a control showing cJun activation. C: Luciferase reporter promoter assays for deletion constructs of the PAK3 promoter region (−2436/+149), (−2329/+149), (−684/+149) and (−179/+149) as well as a (−179/+149) with a mutated cJun/AP-1 binding site at position (+52/+60), in the presence and absence of doxycycline-induced cJun/AP-1 expression. D: Luciferase reporter assays, using the (−179/+149) PAK3 promoter construct, showing the effect of transient cJun inhibition on PAK3 promoter activity in the absence and presence of doxycycline induced cJun/AP-1 over-expression. Results show the mean±S.E. of experiments performed in triplicate and repeated at least three times. (*p≤0.05 and **p≤0.01).

    Article Snippet: PCR using high fidelity Expand Plus DNA Polymerase (Roche) was performed on rat genomic DNA, the products were sub-cloned into the pGEM-T Easy vector (Promega) and excised for cloning into the luciferase reported vector, pGL 3 -Basic (Promega) using Mlu1 and Xho1 restriction enzymes.

    Techniques: Binding Assay, Clone Assay, Plasmid Preparation, Luciferase, Transfection, Construct, Activation Assay, Expressing, Inhibition, Activity Assay, Over Expression