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ATGL functions upstream of <t>PGF</t> <t>2α</t> . (A,B) Percentage of S10B follicles developing in the IVEM assay. In A, data are normalized to wild-type ( yw ) development for the following genotypes: ATGL −/+ ( bmm 1 /+ ), pxt −/+ ( pxt f01000 /+ ) and ATGL/pxt ( bmm 1 /pxt f01000 ). In B, wild-type ( yw ) and ATGL −/− ( bmm 1 /bmm 1 ) follicles were treated with control (ethanol), 1.5 mM aspirin in ethanol, and/or 20 nM PGF 2α analog (fluprostenol, Flu). * P <0.05, ** P <0.005 (unpaired, two-tailed t -test). Error bars represent s.d. ATGL and Pxt interact genetically, as ATGL/pxt follicle development is reduced by ∼50% compared with the ATGL −/+ and pxt −/+ controls (A). Aspirin treatment or loss of ATGL reduces the percentage of follicles developing in control media. In both cases, this is suppressed by exogenously supplying a PGF 2α analog (B).
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ATGL functions upstream of <t>PGF</t> <t>2α</t> . (A,B) Percentage of S10B follicles developing in the IVEM assay. In A, data are normalized to wild-type ( yw ) development for the following genotypes: ATGL −/+ ( bmm 1 /+ ), pxt −/+ ( pxt f01000 /+ ) and ATGL/pxt ( bmm 1 /pxt f01000 ). In B, wild-type ( yw ) and ATGL −/− ( bmm 1 /bmm 1 ) follicles were treated with control (ethanol), 1.5 mM aspirin in ethanol, and/or 20 nM PGF 2α analog (fluprostenol, Flu). * P <0.05, ** P <0.005 (unpaired, two-tailed t -test). Error bars represent s.d. ATGL and Pxt interact genetically, as ATGL/pxt follicle development is reduced by ∼50% compared with the ATGL −/+ and pxt −/+ controls (A). Aspirin treatment or loss of ATGL reduces the percentage of follicles developing in control media. In both cases, this is suppressed by exogenously supplying a PGF 2α analog (B).
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ATGL functions upstream of <t>PGF</t> <t>2α</t> . (A,B) Percentage of S10B follicles developing in the IVEM assay. In A, data are normalized to wild-type ( yw ) development for the following genotypes: ATGL −/+ ( bmm 1 /+ ), pxt −/+ ( pxt f01000 /+ ) and ATGL/pxt ( bmm 1 /pxt f01000 ). In B, wild-type ( yw ) and ATGL −/− ( bmm 1 /bmm 1 ) follicles were treated with control (ethanol), 1.5 mM aspirin in ethanol, and/or 20 nM PGF 2α analog (fluprostenol, Flu). * P <0.05, ** P <0.005 (unpaired, two-tailed t -test). Error bars represent s.d. ATGL and Pxt interact genetically, as ATGL/pxt follicle development is reduced by ∼50% compared with the ATGL −/+ and pxt −/+ controls (A). Aspirin treatment or loss of ATGL reduces the percentage of follicles developing in control media. In both cases, this is suppressed by exogenously supplying a PGF 2α analog (B).
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ATGL functions upstream of <t>PGF</t> <t>2α</t> . (A,B) Percentage of S10B follicles developing in the IVEM assay. In A, data are normalized to wild-type ( yw ) development for the following genotypes: ATGL −/+ ( bmm 1 /+ ), pxt −/+ ( pxt f01000 /+ ) and ATGL/pxt ( bmm 1 /pxt f01000 ). In B, wild-type ( yw ) and ATGL −/− ( bmm 1 /bmm 1 ) follicles were treated with control (ethanol), 1.5 mM aspirin in ethanol, and/or 20 nM PGF 2α analog (fluprostenol, Flu). * P <0.05, ** P <0.005 (unpaired, two-tailed t -test). Error bars represent s.d. ATGL and Pxt interact genetically, as ATGL/pxt follicle development is reduced by ∼50% compared with the ATGL −/+ and pxt −/+ controls (A). Aspirin treatment or loss of ATGL reduces the percentage of follicles developing in control media. In both cases, this is suppressed by exogenously supplying a PGF 2α analog (B).
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ATGL functions upstream of <t>PGF</t> <t>2α</t> . (A,B) Percentage of S10B follicles developing in the IVEM assay. In A, data are normalized to wild-type ( yw ) development for the following genotypes: ATGL −/+ ( bmm 1 /+ ), pxt −/+ ( pxt f01000 /+ ) and ATGL/pxt ( bmm 1 /pxt f01000 ). In B, wild-type ( yw ) and ATGL −/− ( bmm 1 /bmm 1 ) follicles were treated with control (ethanol), 1.5 mM aspirin in ethanol, and/or 20 nM PGF 2α analog (fluprostenol, Flu). * P <0.05, ** P <0.005 (unpaired, two-tailed t -test). Error bars represent s.d. ATGL and Pxt interact genetically, as ATGL/pxt follicle development is reduced by ∼50% compared with the ATGL −/+ and pxt −/+ controls (A). Aspirin treatment or loss of ATGL reduces the percentage of follicles developing in control media. In both cases, this is suppressed by exogenously supplying a PGF 2α analog (B).
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Image Search Results


ATGL functions upstream of PGF 2α . (A,B) Percentage of S10B follicles developing in the IVEM assay. In A, data are normalized to wild-type ( yw ) development for the following genotypes: ATGL −/+ ( bmm 1 /+ ), pxt −/+ ( pxt f01000 /+ ) and ATGL/pxt ( bmm 1 /pxt f01000 ). In B, wild-type ( yw ) and ATGL −/− ( bmm 1 /bmm 1 ) follicles were treated with control (ethanol), 1.5 mM aspirin in ethanol, and/or 20 nM PGF 2α analog (fluprostenol, Flu). * P <0.05, ** P <0.005 (unpaired, two-tailed t -test). Error bars represent s.d. ATGL and Pxt interact genetically, as ATGL/pxt follicle development is reduced by ∼50% compared with the ATGL −/+ and pxt −/+ controls (A). Aspirin treatment or loss of ATGL reduces the percentage of follicles developing in control media. In both cases, this is suppressed by exogenously supplying a PGF 2α analog (B).

Journal: Development (Cambridge, England)

Article Title: Adipose triglyceride lipase promotes prostaglandin-dependent actin remodeling by regulating substrate release from lipid droplets

doi: 10.1242/dev.201516

Figure Lengend Snippet: ATGL functions upstream of PGF 2α . (A,B) Percentage of S10B follicles developing in the IVEM assay. In A, data are normalized to wild-type ( yw ) development for the following genotypes: ATGL −/+ ( bmm 1 /+ ), pxt −/+ ( pxt f01000 /+ ) and ATGL/pxt ( bmm 1 /pxt f01000 ). In B, wild-type ( yw ) and ATGL −/− ( bmm 1 /bmm 1 ) follicles were treated with control (ethanol), 1.5 mM aspirin in ethanol, and/or 20 nM PGF 2α analog (fluprostenol, Flu). * P <0.05, ** P <0.005 (unpaired, two-tailed t -test). Error bars represent s.d. ATGL and Pxt interact genetically, as ATGL/pxt follicle development is reduced by ∼50% compared with the ATGL −/+ and pxt −/+ controls (A). Aspirin treatment or loss of ATGL reduces the percentage of follicles developing in control media. In both cases, this is suppressed by exogenously supplying a PGF 2α analog (B).

Article Snippet: For the exogenous PGF 2α analog [Fluprostanol (Flu), Cayman Chemical Company] experiments, the standard IVEM was performed and stock solutions of 10 μM Flu and 0.5 M aspirin (Cayman Chemical Company) were prepared in 100% ethanol.

Techniques: Control, Two Tailed Test