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Agilent technologies pgc 1α probe
Reduced number of main arteries and veins in <t>PGC-1α</t> −/− mice. A : Flat-mounted retinas of WT and PGC-1α knockout littermates at P13.5 were costained with isolectin B4 (green) and smooth-muscle actin (red) to differentiate veins (v) and arteries (a). The number of main arteries and veins was reduced in PGC-1α −/− ( B ) (mean ± SD, n = 7 to 11) * P
Pgc 1α Probe, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgc 1α probe/product/Agilent technologies
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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86/100 stars

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1) Product Images from "PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina"

Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

Journal: The American Journal of Pathology

doi: 10.1016/j.ajpath.2012.09.003

Reduced number of main arteries and veins in PGC-1α −/− mice. A : Flat-mounted retinas of WT and PGC-1α knockout littermates at P13.5 were costained with isolectin B4 (green) and smooth-muscle actin (red) to differentiate veins (v) and arteries (a). The number of main arteries and veins was reduced in PGC-1α −/− ( B ) (mean ± SD, n = 7 to 11) * P
Figure Legend Snippet: Reduced number of main arteries and veins in PGC-1α −/− mice. A : Flat-mounted retinas of WT and PGC-1α knockout littermates at P13.5 were costained with isolectin B4 (green) and smooth-muscle actin (red) to differentiate veins (v) and arteries (a). The number of main arteries and veins was reduced in PGC-1α −/− ( B ) (mean ± SD, n = 7 to 11) * P

Techniques Used: Pyrolysis Gas Chromatography, Mouse Assay, Knock-Out

PGC-1α regulates VEGFA in retinal cells. RGC5 ( A ), 661W ( B ), ARPE-19 ( C ), and MIO-M1 ( D ) were infected with adenovirus expressing mouse PGC-1α or GFP (as negative control). Forty-eight hours postinfection, RNA was isolated and gene expression measured by qPCR. VEGFA was up-regulated two- to threefold in all cells. MIO-M1 were infected with retrovirus carrying vacant or mouse PGC-1α plasmids. PGC-1α expression at the protein level was elevated with induction of its target genes ( E ). PGC-1α–dependent up-regulation ( F ) and secretion ( G ) of VEGF in MIO-M1 cells is enhanced by hypoxia. PGC-1α knockdown by shRNA inhibits VEGFA expression in normoxic and hypoxic conditions ( H ). * P
Figure Legend Snippet: PGC-1α regulates VEGFA in retinal cells. RGC5 ( A ), 661W ( B ), ARPE-19 ( C ), and MIO-M1 ( D ) were infected with adenovirus expressing mouse PGC-1α or GFP (as negative control). Forty-eight hours postinfection, RNA was isolated and gene expression measured by qPCR. VEGFA was up-regulated two- to threefold in all cells. MIO-M1 were infected with retrovirus carrying vacant or mouse PGC-1α plasmids. PGC-1α expression at the protein level was elevated with induction of its target genes ( E ). PGC-1α–dependent up-regulation ( F ) and secretion ( G ) of VEGF in MIO-M1 cells is enhanced by hypoxia. PGC-1α knockdown by shRNA inhibits VEGFA expression in normoxic and hypoxic conditions ( H ). * P

Techniques Used: Pyrolysis Gas Chromatography, Infection, Expressing, Negative Control, Isolation, Real-time Polymerase Chain Reaction, shRNA

PGC-1α −/− mice are protected against OIR. Oxygen-induced retinopathy (OIR) was induced by exposing PGC-1α +/+ (WT) and PGC-1α −/− mouse pups to 75% oxygen from P7 to P12. A and B : At P17, retinas were harvested and stained with isolectin-B4. The vaso-obliterated areas ( yellow outline ) and neovascular areas (marked in white) were quantified using Photoshop CS4 software. Loss of PGC-1α did not affect the avascular zone but significantly reduced the neovascular area ( n = 7 to 9). ** P
Figure Legend Snippet: PGC-1α −/− mice are protected against OIR. Oxygen-induced retinopathy (OIR) was induced by exposing PGC-1α +/+ (WT) and PGC-1α −/− mouse pups to 75% oxygen from P7 to P12. A and B : At P17, retinas were harvested and stained with isolectin-B4. The vaso-obliterated areas ( yellow outline ) and neovascular areas (marked in white) were quantified using Photoshop CS4 software. Loss of PGC-1α did not affect the avascular zone but significantly reduced the neovascular area ( n = 7 to 9). ** P

Techniques Used: Pyrolysis Gas Chromatography, Mouse Assay, Staining, Software

Delayed retinal vascular development and decreased capillary density in PGC-1α −/− mice. Developmental retinal vessels outgrowth was evaluated at P4 on isolectin-B4 (green) and/or collagen IV (red) stained flat-mounts ( A ) and quantified ( B ). PGC-1α −/− pups showed reduced vascular radius ( red arrows ) and area compared to WT littermates ( A and B ). High-magnification micrographs of the vascular front revealed a decreased number of tip cells ( asterisks ) in PGC-1α −/− compared to WT ( A ). Vascular density was quantified on collagen IV–stained P4 retina flat-mounts and revealed a significant decrease of capillary density in PGC-1α −/− mice ( n = 5 to 7). * P
Figure Legend Snippet: Delayed retinal vascular development and decreased capillary density in PGC-1α −/− mice. Developmental retinal vessels outgrowth was evaluated at P4 on isolectin-B4 (green) and/or collagen IV (red) stained flat-mounts ( A ) and quantified ( B ). PGC-1α −/− pups showed reduced vascular radius ( red arrows ) and area compared to WT littermates ( A and B ). High-magnification micrographs of the vascular front revealed a decreased number of tip cells ( asterisks ) in PGC-1α −/− compared to WT ( A ). Vascular density was quantified on collagen IV–stained P4 retina flat-mounts and revealed a significant decrease of capillary density in PGC-1α −/− mice ( n = 5 to 7). * P

Techniques Used: Pyrolysis Gas Chromatography, Mouse Assay, Staining

Region-specific regulation of PGC-1α expression during OIR. A : Schematic representation of the OIR model. B : qPCR quantification of PGC-1α and VEGFA mRNA in total retinas from P12 to P17 in control (white bars) and OIR (black bars) WT mice. C : Representative pictures of retinal sections before (Pre LCM) and after (Post LCM) laser capture microdissection. D : mRNA quantification by qPCR from laser-captured ONL, INL, and GCL of P17 control and OIR retinas (log y axis). Results are expressed as RE (relative expression) normalized to housekeeping genes (mean ± SEM, n = 3 to 4). * P
Figure Legend Snippet: Region-specific regulation of PGC-1α expression during OIR. A : Schematic representation of the OIR model. B : qPCR quantification of PGC-1α and VEGFA mRNA in total retinas from P12 to P17 in control (white bars) and OIR (black bars) WT mice. C : Representative pictures of retinal sections before (Pre LCM) and after (Post LCM) laser capture microdissection. D : mRNA quantification by qPCR from laser-captured ONL, INL, and GCL of P17 control and OIR retinas (log y axis). Results are expressed as RE (relative expression) normalized to housekeeping genes (mean ± SEM, n = 3 to 4). * P

Techniques Used: Pyrolysis Gas Chromatography, Expressing, Real-time Polymerase Chain Reaction, Mouse Assay, Laser Capture Microdissection

PGC-1α expression in the retina and during retinal development. A : RNA from the indicated adult murine tissues was extracted and subjected to RT-qPCR for PGC-1α. B – D : RNA from retinas of WT mice at age postnatal day 1 (P1) to P17 were harvested and subjected to RT-qPCR. The expression levels of PGC-1α ( B ) and its downstream target genes, CYCS and MCAD ( C ), were increased with development with a surge from P3 to P7. The developmental expression curve of PGC-1α also correlated with VEGFA, PDGFB, and ANG2 expression during the early stage of vascular outgrowth (up to P7). However, high PGC-1α expression is maintained during the later stage of retinal development, whereas VEGFA expression is repressed ( D ). * P
Figure Legend Snippet: PGC-1α expression in the retina and during retinal development. A : RNA from the indicated adult murine tissues was extracted and subjected to RT-qPCR for PGC-1α. B – D : RNA from retinas of WT mice at age postnatal day 1 (P1) to P17 were harvested and subjected to RT-qPCR. The expression levels of PGC-1α ( B ) and its downstream target genes, CYCS and MCAD ( C ), were increased with development with a surge from P3 to P7. The developmental expression curve of PGC-1α also correlated with VEGFA, PDGFB, and ANG2 expression during the early stage of vascular outgrowth (up to P7). However, high PGC-1α expression is maintained during the later stage of retinal development, whereas VEGFA expression is repressed ( D ). * P

Techniques Used: Pyrolysis Gas Chromatography, Expressing, Quantitative RT-PCR, Mouse Assay

Normal ocular and retinal structures in PGC-1α −/− mice. A and B : H E-stained sagittal ocular sections from 8-week-old PGC-1α +/+ (WT) and PGC-1α −/− mice. A : Low magnification showing normal ocular structures; scale bars = 500 μm. B : Higher magnification showing no abnormal retinal organization or layers thickness in PGC-1α −/− mice; scale bars = 50 μm. C : Representative pictograms of SD-OCT recordings from WT and PGC-1α −/− mice, the ONL is indicated by double-ended arrows . Scale bars = 100 μm. D : ONL thickness from OCT recordings were quantified every 100 μm from the optic nerve head (OD) (mean ± SD, n = 3 to 6). GCL, ganglion cell layer; INL, inner nuclear layer; IS, inner segment; ONL, outer nuclear layer; OS, outer segment.
Figure Legend Snippet: Normal ocular and retinal structures in PGC-1α −/− mice. A and B : H E-stained sagittal ocular sections from 8-week-old PGC-1α +/+ (WT) and PGC-1α −/− mice. A : Low magnification showing normal ocular structures; scale bars = 500 μm. B : Higher magnification showing no abnormal retinal organization or layers thickness in PGC-1α −/− mice; scale bars = 50 μm. C : Representative pictograms of SD-OCT recordings from WT and PGC-1α −/− mice, the ONL is indicated by double-ended arrows . Scale bars = 100 μm. D : ONL thickness from OCT recordings were quantified every 100 μm from the optic nerve head (OD) (mean ± SD, n = 3 to 6). GCL, ganglion cell layer; INL, inner nuclear layer; IS, inner segment; ONL, outer nuclear layer; OS, outer segment.

Techniques Used: Pyrolysis Gas Chromatography, Mouse Assay, Staining

Related Articles

Plasmid Preparation:

Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina
Article Snippet: .. PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin. .. Flat-mounted retinas were hybridized and stained following the method previously described, with some modifications.

Subcloning:

Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina
Article Snippet: .. PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin. .. Flat-mounted retinas were hybridized and stained following the method previously described, with some modifications.

Pyrolysis Gas Chromatography:

Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina
Article Snippet: .. PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin. .. Flat-mounted retinas were hybridized and stained following the method previously described, with some modifications.

Labeling:

Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina
Article Snippet: .. PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin. .. Flat-mounted retinas were hybridized and stained following the method previously described, with some modifications.

In Vitro:

Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina
Article Snippet: .. PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin. .. Flat-mounted retinas were hybridized and stained following the method previously described, with some modifications.

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    Agilent technologies pgc 1α probe
    Reduced number of main arteries and veins in <t>PGC-1α</t> −/− mice. A : Flat-mounted retinas of WT and PGC-1α knockout littermates at P13.5 were costained with isolectin B4 (green) and smooth-muscle actin (red) to differentiate veins (v) and arteries (a). The number of main arteries and veins was reduced in PGC-1α −/− ( B ) (mean ± SD, n = 7 to 11) * P
    Pgc 1α Probe, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgc 1α probe/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pgc 1α probe - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

    91
    Agilent technologies pgc 1α mutant cdna
    <t>PGC-1α</t> knockdown inhibits T3-induced dendritic growth in Purkinje cells. (A) HEK293T cells expressing mouse PGC-1α were transfected with PGC-1α-scramble (scr) or short hairpin RNA (shRNA) construct and analyzed by western blotting with anti-PGC-1α and anti-β-actin antibodies. (B,C) Cultured Purkinje cells were transfected with PGC-1α-shRNA or scr-shRNA (control) constructs and stained for Calbindin and PGC-1α (B) or Calbindin and cytochrome C oxidase IV (COX-IV) (C) at 10 DIV. Scale bars, 10 μm. (D) Representative images of Purkinje cells transfected with scr shRNA (control), PGC-1α shRNA (PGC-1α shRNA), or PGC-1α shRNA plus an shRNA-resistant mutant of PGC-1α (shRNA + rescue). Cells were cultured in the presence of 10 nM T3 until 10 DIV and immunostained with anti-Calbindin and anti-PDH antibodies. Boxed regions in the upper panels are enlarged in lower panels. Scale bars, 20 μm. (E–G) Quantitative analyses of the total dendritic length (E) , number of dendritic branches (F) and PDH signal (G) . Data represent mean ± SEM, N = 30 for each point, ** p
    Pgc 1α Mutant Cdna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgc 1α mutant cdna/product/Agilent technologies
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pgc 1α mutant cdna - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    86
    Agilent technologies mouse pgc 1α cdna
    Reduced number of main arteries and veins in <t>PGC-1α</t> −/− mice. A : Flat-mounted retinas of WT and PGC-1α knockout littermates at P13.5 were costained with isolectin B4 (green) and smooth-muscle actin (red) to differentiate veins (v) and arteries (a). The number of main arteries and veins was reduced in PGC-1α −/− ( B ) (mean ± SD, n = 7 to 11) * P
    Mouse Pgc 1α Cdna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse pgc 1α cdna/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse pgc 1α cdna - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

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    Reduced number of main arteries and veins in PGC-1α −/− mice. A : Flat-mounted retinas of WT and PGC-1α knockout littermates at P13.5 were costained with isolectin B4 (green) and smooth-muscle actin (red) to differentiate veins (v) and arteries (a). The number of main arteries and veins was reduced in PGC-1α −/− ( B ) (mean ± SD, n = 7 to 11) * P

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: Reduced number of main arteries and veins in PGC-1α −/− mice. A : Flat-mounted retinas of WT and PGC-1α knockout littermates at P13.5 were costained with isolectin B4 (green) and smooth-muscle actin (red) to differentiate veins (v) and arteries (a). The number of main arteries and veins was reduced in PGC-1α −/− ( B ) (mean ± SD, n = 7 to 11) * P

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Mouse Assay, Knock-Out

    PGC-1α regulates VEGFA in retinal cells. RGC5 ( A ), 661W ( B ), ARPE-19 ( C ), and MIO-M1 ( D ) were infected with adenovirus expressing mouse PGC-1α or GFP (as negative control). Forty-eight hours postinfection, RNA was isolated and gene expression measured by qPCR. VEGFA was up-regulated two- to threefold in all cells. MIO-M1 were infected with retrovirus carrying vacant or mouse PGC-1α plasmids. PGC-1α expression at the protein level was elevated with induction of its target genes ( E ). PGC-1α–dependent up-regulation ( F ) and secretion ( G ) of VEGF in MIO-M1 cells is enhanced by hypoxia. PGC-1α knockdown by shRNA inhibits VEGFA expression in normoxic and hypoxic conditions ( H ). * P

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: PGC-1α regulates VEGFA in retinal cells. RGC5 ( A ), 661W ( B ), ARPE-19 ( C ), and MIO-M1 ( D ) were infected with adenovirus expressing mouse PGC-1α or GFP (as negative control). Forty-eight hours postinfection, RNA was isolated and gene expression measured by qPCR. VEGFA was up-regulated two- to threefold in all cells. MIO-M1 were infected with retrovirus carrying vacant or mouse PGC-1α plasmids. PGC-1α expression at the protein level was elevated with induction of its target genes ( E ). PGC-1α–dependent up-regulation ( F ) and secretion ( G ) of VEGF in MIO-M1 cells is enhanced by hypoxia. PGC-1α knockdown by shRNA inhibits VEGFA expression in normoxic and hypoxic conditions ( H ). * P

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Infection, Expressing, Negative Control, Isolation, Real-time Polymerase Chain Reaction, shRNA

    PGC-1α −/− mice are protected against OIR. Oxygen-induced retinopathy (OIR) was induced by exposing PGC-1α +/+ (WT) and PGC-1α −/− mouse pups to 75% oxygen from P7 to P12. A and B : At P17, retinas were harvested and stained with isolectin-B4. The vaso-obliterated areas ( yellow outline ) and neovascular areas (marked in white) were quantified using Photoshop CS4 software. Loss of PGC-1α did not affect the avascular zone but significantly reduced the neovascular area ( n = 7 to 9). ** P

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: PGC-1α −/− mice are protected against OIR. Oxygen-induced retinopathy (OIR) was induced by exposing PGC-1α +/+ (WT) and PGC-1α −/− mouse pups to 75% oxygen from P7 to P12. A and B : At P17, retinas were harvested and stained with isolectin-B4. The vaso-obliterated areas ( yellow outline ) and neovascular areas (marked in white) were quantified using Photoshop CS4 software. Loss of PGC-1α did not affect the avascular zone but significantly reduced the neovascular area ( n = 7 to 9). ** P

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Mouse Assay, Staining, Software

    Delayed retinal vascular development and decreased capillary density in PGC-1α −/− mice. Developmental retinal vessels outgrowth was evaluated at P4 on isolectin-B4 (green) and/or collagen IV (red) stained flat-mounts ( A ) and quantified ( B ). PGC-1α −/− pups showed reduced vascular radius ( red arrows ) and area compared to WT littermates ( A and B ). High-magnification micrographs of the vascular front revealed a decreased number of tip cells ( asterisks ) in PGC-1α −/− compared to WT ( A ). Vascular density was quantified on collagen IV–stained P4 retina flat-mounts and revealed a significant decrease of capillary density in PGC-1α −/− mice ( n = 5 to 7). * P

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: Delayed retinal vascular development and decreased capillary density in PGC-1α −/− mice. Developmental retinal vessels outgrowth was evaluated at P4 on isolectin-B4 (green) and/or collagen IV (red) stained flat-mounts ( A ) and quantified ( B ). PGC-1α −/− pups showed reduced vascular radius ( red arrows ) and area compared to WT littermates ( A and B ). High-magnification micrographs of the vascular front revealed a decreased number of tip cells ( asterisks ) in PGC-1α −/− compared to WT ( A ). Vascular density was quantified on collagen IV–stained P4 retina flat-mounts and revealed a significant decrease of capillary density in PGC-1α −/− mice ( n = 5 to 7). * P

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Mouse Assay, Staining

    Region-specific regulation of PGC-1α expression during OIR. A : Schematic representation of the OIR model. B : qPCR quantification of PGC-1α and VEGFA mRNA in total retinas from P12 to P17 in control (white bars) and OIR (black bars) WT mice. C : Representative pictures of retinal sections before (Pre LCM) and after (Post LCM) laser capture microdissection. D : mRNA quantification by qPCR from laser-captured ONL, INL, and GCL of P17 control and OIR retinas (log y axis). Results are expressed as RE (relative expression) normalized to housekeeping genes (mean ± SEM, n = 3 to 4). * P

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: Region-specific regulation of PGC-1α expression during OIR. A : Schematic representation of the OIR model. B : qPCR quantification of PGC-1α and VEGFA mRNA in total retinas from P12 to P17 in control (white bars) and OIR (black bars) WT mice. C : Representative pictures of retinal sections before (Pre LCM) and after (Post LCM) laser capture microdissection. D : mRNA quantification by qPCR from laser-captured ONL, INL, and GCL of P17 control and OIR retinas (log y axis). Results are expressed as RE (relative expression) normalized to housekeeping genes (mean ± SEM, n = 3 to 4). * P

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Expressing, Real-time Polymerase Chain Reaction, Mouse Assay, Laser Capture Microdissection

    PGC-1α expression in the retina and during retinal development. A : RNA from the indicated adult murine tissues was extracted and subjected to RT-qPCR for PGC-1α. B – D : RNA from retinas of WT mice at age postnatal day 1 (P1) to P17 were harvested and subjected to RT-qPCR. The expression levels of PGC-1α ( B ) and its downstream target genes, CYCS and MCAD ( C ), were increased with development with a surge from P3 to P7. The developmental expression curve of PGC-1α also correlated with VEGFA, PDGFB, and ANG2 expression during the early stage of vascular outgrowth (up to P7). However, high PGC-1α expression is maintained during the later stage of retinal development, whereas VEGFA expression is repressed ( D ). * P

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: PGC-1α expression in the retina and during retinal development. A : RNA from the indicated adult murine tissues was extracted and subjected to RT-qPCR for PGC-1α. B – D : RNA from retinas of WT mice at age postnatal day 1 (P1) to P17 were harvested and subjected to RT-qPCR. The expression levels of PGC-1α ( B ) and its downstream target genes, CYCS and MCAD ( C ), were increased with development with a surge from P3 to P7. The developmental expression curve of PGC-1α also correlated with VEGFA, PDGFB, and ANG2 expression during the early stage of vascular outgrowth (up to P7). However, high PGC-1α expression is maintained during the later stage of retinal development, whereas VEGFA expression is repressed ( D ). * P

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Expressing, Quantitative RT-PCR, Mouse Assay

    Normal ocular and retinal structures in PGC-1α −/− mice. A and B : H E-stained sagittal ocular sections from 8-week-old PGC-1α +/+ (WT) and PGC-1α −/− mice. A : Low magnification showing normal ocular structures; scale bars = 500 μm. B : Higher magnification showing no abnormal retinal organization or layers thickness in PGC-1α −/− mice; scale bars = 50 μm. C : Representative pictograms of SD-OCT recordings from WT and PGC-1α −/− mice, the ONL is indicated by double-ended arrows . Scale bars = 100 μm. D : ONL thickness from OCT recordings were quantified every 100 μm from the optic nerve head (OD) (mean ± SD, n = 3 to 6). GCL, ganglion cell layer; INL, inner nuclear layer; IS, inner segment; ONL, outer nuclear layer; OS, outer segment.

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: Normal ocular and retinal structures in PGC-1α −/− mice. A and B : H E-stained sagittal ocular sections from 8-week-old PGC-1α +/+ (WT) and PGC-1α −/− mice. A : Low magnification showing normal ocular structures; scale bars = 500 μm. B : Higher magnification showing no abnormal retinal organization or layers thickness in PGC-1α −/− mice; scale bars = 50 μm. C : Representative pictograms of SD-OCT recordings from WT and PGC-1α −/− mice, the ONL is indicated by double-ended arrows . Scale bars = 100 μm. D : ONL thickness from OCT recordings were quantified every 100 μm from the optic nerve head (OD) (mean ± SD, n = 3 to 6). GCL, ganglion cell layer; INL, inner nuclear layer; IS, inner segment; ONL, outer nuclear layer; OS, outer segment.

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Mouse Assay, Staining

    PGC-1α knockdown inhibits T3-induced dendritic growth in Purkinje cells. (A) HEK293T cells expressing mouse PGC-1α were transfected with PGC-1α-scramble (scr) or short hairpin RNA (shRNA) construct and analyzed by western blotting with anti-PGC-1α and anti-β-actin antibodies. (B,C) Cultured Purkinje cells were transfected with PGC-1α-shRNA or scr-shRNA (control) constructs and stained for Calbindin and PGC-1α (B) or Calbindin and cytochrome C oxidase IV (COX-IV) (C) at 10 DIV. Scale bars, 10 μm. (D) Representative images of Purkinje cells transfected with scr shRNA (control), PGC-1α shRNA (PGC-1α shRNA), or PGC-1α shRNA plus an shRNA-resistant mutant of PGC-1α (shRNA + rescue). Cells were cultured in the presence of 10 nM T3 until 10 DIV and immunostained with anti-Calbindin and anti-PDH antibodies. Boxed regions in the upper panels are enlarged in lower panels. Scale bars, 20 μm. (E–G) Quantitative analyses of the total dendritic length (E) , number of dendritic branches (F) and PDH signal (G) . Data represent mean ± SEM, N = 30 for each point, ** p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Thyroid Hormone Induces PGC-1α during Dendritic Outgrowth in Mouse Cerebellar Purkinje Cells

    doi: 10.3389/fncel.2017.00133

    Figure Lengend Snippet: PGC-1α knockdown inhibits T3-induced dendritic growth in Purkinje cells. (A) HEK293T cells expressing mouse PGC-1α were transfected with PGC-1α-scramble (scr) or short hairpin RNA (shRNA) construct and analyzed by western blotting with anti-PGC-1α and anti-β-actin antibodies. (B,C) Cultured Purkinje cells were transfected with PGC-1α-shRNA or scr-shRNA (control) constructs and stained for Calbindin and PGC-1α (B) or Calbindin and cytochrome C oxidase IV (COX-IV) (C) at 10 DIV. Scale bars, 10 μm. (D) Representative images of Purkinje cells transfected with scr shRNA (control), PGC-1α shRNA (PGC-1α shRNA), or PGC-1α shRNA plus an shRNA-resistant mutant of PGC-1α (shRNA + rescue). Cells were cultured in the presence of 10 nM T3 until 10 DIV and immunostained with anti-Calbindin and anti-PDH antibodies. Boxed regions in the upper panels are enlarged in lower panels. Scale bars, 20 μm. (E–G) Quantitative analyses of the total dendritic length (E) , number of dendritic branches (F) and PDH signal (G) . Data represent mean ± SEM, N = 30 for each point, ** p

    Article Snippet: PGC-1α mutant cDNA, which contains three silent mutations introduced in the respective short hairpin RNA (shRNA) target sequence, was generated using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies).

    Techniques: Pyrolysis Gas Chromatography, Expressing, Transfection, shRNA, Construct, Western Blot, Cell Culture, Staining, Mutagenesis

    PGC-1α overexpression enhances dendritic outgrowth of Purkinje cells in the absence of T3. (A,B) The morphology of Purkinje cells transfected with tdTomato (control) or PGC-1α-mCherry (+PGC-1α). Cells were cultured with (B) or without (A) T3 and stained for Calbindin at 10 DIV. Scale bars, 20 μm. PGC-1α overexpression induced dendritic outgrowth in the absence of T3 (A) , but not in the presence of T3 (B) . (C–E) Quantitative analyses of total dendritic length (C) branch numbers (D) and PDH signal (E) . Purkinje cells expressing tdTomato (control) or PGC-1α were cultured with or without T3 treatment. N = 30 for all data points. Data represent mean ± SEM, *** p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Thyroid Hormone Induces PGC-1α during Dendritic Outgrowth in Mouse Cerebellar Purkinje Cells

    doi: 10.3389/fncel.2017.00133

    Figure Lengend Snippet: PGC-1α overexpression enhances dendritic outgrowth of Purkinje cells in the absence of T3. (A,B) The morphology of Purkinje cells transfected with tdTomato (control) or PGC-1α-mCherry (+PGC-1α). Cells were cultured with (B) or without (A) T3 and stained for Calbindin at 10 DIV. Scale bars, 20 μm. PGC-1α overexpression induced dendritic outgrowth in the absence of T3 (A) , but not in the presence of T3 (B) . (C–E) Quantitative analyses of total dendritic length (C) branch numbers (D) and PDH signal (E) . Purkinje cells expressing tdTomato (control) or PGC-1α were cultured with or without T3 treatment. N = 30 for all data points. Data represent mean ± SEM, *** p

    Article Snippet: PGC-1α mutant cDNA, which contains three silent mutations introduced in the respective short hairpin RNA (shRNA) target sequence, was generated using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies).

    Techniques: Pyrolysis Gas Chromatography, Over Expression, Transfection, Cell Culture, Staining, Expressing

    Knockdown of PGC-1α inhibits dendritic outgrowth in vivo Purkinje cells. (A) Representative images of Purkinje cells transfected with scr shRNA (control) or PGC-1α shRNA construct. Scale bar, 20 μm. (B) Dual color images of GFP derived from shRNA constructs (green) and immunostaining with anti-PGC-1α (magenta). Scale bar, 20 μm. (C,D) Quantitative analyses of the total dendritic length (C) and number of dendritic branches (D) in Purkinje cells expressing scr shRNA (control) or PGC-1α shRNA constructs. Data represent mean ± SEM, N = 12 cells from three mice, *** p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Thyroid Hormone Induces PGC-1α during Dendritic Outgrowth in Mouse Cerebellar Purkinje Cells

    doi: 10.3389/fncel.2017.00133

    Figure Lengend Snippet: Knockdown of PGC-1α inhibits dendritic outgrowth in vivo Purkinje cells. (A) Representative images of Purkinje cells transfected with scr shRNA (control) or PGC-1α shRNA construct. Scale bar, 20 μm. (B) Dual color images of GFP derived from shRNA constructs (green) and immunostaining with anti-PGC-1α (magenta). Scale bar, 20 μm. (C,D) Quantitative analyses of the total dendritic length (C) and number of dendritic branches (D) in Purkinje cells expressing scr shRNA (control) or PGC-1α shRNA constructs. Data represent mean ± SEM, N = 12 cells from three mice, *** p

    Article Snippet: PGC-1α mutant cDNA, which contains three silent mutations introduced in the respective short hairpin RNA (shRNA) target sequence, was generated using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies).

    Techniques: Pyrolysis Gas Chromatography, In Vivo, Transfection, shRNA, Construct, Derivative Assay, Immunostaining, Expressing, Mouse Assay

    PGC-1α expression in the developing cerebellar cortex. (A) Shape changes of Purkinje cell dendrites during postnatal development. (B,C) Sagittal cerebellar sections were immunostained for PGC-1α and Calbindin at different ages of development and were observed at low (B) and high (C) magnification. Scale bars, 40 μm (B) and 20 μm (C) . PGC-1α was predominantly detected in the Purkinje cells from P7. EGL, external granule layer; ML, molecular layer; PCL, Purkinje cell layer; IGL, internal granule layer; WM, white matter.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Thyroid Hormone Induces PGC-1α during Dendritic Outgrowth in Mouse Cerebellar Purkinje Cells

    doi: 10.3389/fncel.2017.00133

    Figure Lengend Snippet: PGC-1α expression in the developing cerebellar cortex. (A) Shape changes of Purkinje cell dendrites during postnatal development. (B,C) Sagittal cerebellar sections were immunostained for PGC-1α and Calbindin at different ages of development and were observed at low (B) and high (C) magnification. Scale bars, 40 μm (B) and 20 μm (C) . PGC-1α was predominantly detected in the Purkinje cells from P7. EGL, external granule layer; ML, molecular layer; PCL, Purkinje cell layer; IGL, internal granule layer; WM, white matter.

    Article Snippet: PGC-1α mutant cDNA, which contains three silent mutations introduced in the respective short hairpin RNA (shRNA) target sequence, was generated using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies).

    Techniques: Pyrolysis Gas Chromatography, Expressing

    PGC-1α expression in Purkinje cells is downregulated in hypothyroid mice. (A–C) PGC-1α expression was compared at P7 (A) , P9 (B) and P14 (C) . Representative images from four mice in each condition are shown. Dendritic growth of Purkinje cells is retarded in the hypothyroid condition. At P14 (C) , the EGL is thicker in hypothyroid animals compared to control animals. Each section was immunostained with anti-Calbindin and anti-PGC-1α. DNA was counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bars, 40 μm. (D) Quantitative comparison of PGC-1α expression in Purkinje cells in control (euthyroid) and hypothyroid animals. Data represent mean ± SEM, N = 15 cells from four mice from two independent experiments for each points, * p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Thyroid Hormone Induces PGC-1α during Dendritic Outgrowth in Mouse Cerebellar Purkinje Cells

    doi: 10.3389/fncel.2017.00133

    Figure Lengend Snippet: PGC-1α expression in Purkinje cells is downregulated in hypothyroid mice. (A–C) PGC-1α expression was compared at P7 (A) , P9 (B) and P14 (C) . Representative images from four mice in each condition are shown. Dendritic growth of Purkinje cells is retarded in the hypothyroid condition. At P14 (C) , the EGL is thicker in hypothyroid animals compared to control animals. Each section was immunostained with anti-Calbindin and anti-PGC-1α. DNA was counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bars, 40 μm. (D) Quantitative comparison of PGC-1α expression in Purkinje cells in control (euthyroid) and hypothyroid animals. Data represent mean ± SEM, N = 15 cells from four mice from two independent experiments for each points, * p

    Article Snippet: PGC-1α mutant cDNA, which contains three silent mutations introduced in the respective short hairpin RNA (shRNA) target sequence, was generated using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies).

    Techniques: Pyrolysis Gas Chromatography, Expressing, Mouse Assay

    PGC-1α expression is induced in cultured Purkinje cells by T3 treatment. (A) Cerebellar cells were cultured in the presence of 10 nM T3 and then immunostained with anti-Calbindin and PGC-1α at the indicated day in culture. Boxed regions in the upper panels are enlarged in lower panels. PGC-1α expression is gradually increased in Purkinje cells (arrows) from 6 DIV. Scale bars, 20 μm. (B) Quantitative comparison of PGC-1α expression in Purkinje cells cultured with (black dots and line) or without (gray dots and line) T3. Data represent mean ± SEM, N = 15 cells for each points, *** p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Thyroid Hormone Induces PGC-1α during Dendritic Outgrowth in Mouse Cerebellar Purkinje Cells

    doi: 10.3389/fncel.2017.00133

    Figure Lengend Snippet: PGC-1α expression is induced in cultured Purkinje cells by T3 treatment. (A) Cerebellar cells were cultured in the presence of 10 nM T3 and then immunostained with anti-Calbindin and PGC-1α at the indicated day in culture. Boxed regions in the upper panels are enlarged in lower panels. PGC-1α expression is gradually increased in Purkinje cells (arrows) from 6 DIV. Scale bars, 20 μm. (B) Quantitative comparison of PGC-1α expression in Purkinje cells cultured with (black dots and line) or without (gray dots and line) T3. Data represent mean ± SEM, N = 15 cells for each points, *** p

    Article Snippet: PGC-1α mutant cDNA, which contains three silent mutations introduced in the respective short hairpin RNA (shRNA) target sequence, was generated using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies).

    Techniques: Pyrolysis Gas Chromatography, Expressing, Cell Culture

    Molecular perturbation of PGC-1α inhibits dendritic outgrowth and mitochondrial activity in Purkinje cells. (A) Representative images of Purkinje cells overexpressing EGFP (control), EGFP-NRF1DN or FLAG-RIP140. Cells were stained for Calbindin at 10 DIV. Scale bar, 20 μm. (B–D) Quantitative analyses of the total dendritic length (B) , number of dendritic branches (C) and PDH signal (D) . N = 40 cells for control, 30 cells for NRF1DN and 30 cells for RIP140. Data represent mean ± SEM, ** p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Thyroid Hormone Induces PGC-1α during Dendritic Outgrowth in Mouse Cerebellar Purkinje Cells

    doi: 10.3389/fncel.2017.00133

    Figure Lengend Snippet: Molecular perturbation of PGC-1α inhibits dendritic outgrowth and mitochondrial activity in Purkinje cells. (A) Representative images of Purkinje cells overexpressing EGFP (control), EGFP-NRF1DN or FLAG-RIP140. Cells were stained for Calbindin at 10 DIV. Scale bar, 20 μm. (B–D) Quantitative analyses of the total dendritic length (B) , number of dendritic branches (C) and PDH signal (D) . N = 40 cells for control, 30 cells for NRF1DN and 30 cells for RIP140. Data represent mean ± SEM, ** p

    Article Snippet: PGC-1α mutant cDNA, which contains three silent mutations introduced in the respective short hairpin RNA (shRNA) target sequence, was generated using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies).

    Techniques: Pyrolysis Gas Chromatography, Activity Assay, Staining

    Reduced number of main arteries and veins in PGC-1α −/− mice. A : Flat-mounted retinas of WT and PGC-1α knockout littermates at P13.5 were costained with isolectin B4 (green) and smooth-muscle actin (red) to differentiate veins (v) and arteries (a). The number of main arteries and veins was reduced in PGC-1α −/− ( B ) (mean ± SD, n = 7 to 11) * P

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: Reduced number of main arteries and veins in PGC-1α −/− mice. A : Flat-mounted retinas of WT and PGC-1α knockout littermates at P13.5 were costained with isolectin B4 (green) and smooth-muscle actin (red) to differentiate veins (v) and arteries (a). The number of main arteries and veins was reduced in PGC-1α −/− ( B ) (mean ± SD, n = 7 to 11) * P

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Mouse Assay, Knock-Out

    PGC-1α regulates VEGFA in retinal cells. RGC5 ( A ), 661W ( B ), ARPE-19 ( C ), and MIO-M1 ( D ) were infected with adenovirus expressing mouse PGC-1α or GFP (as negative control). Forty-eight hours postinfection, RNA was isolated and gene expression measured by qPCR. VEGFA was up-regulated two- to threefold in all cells. MIO-M1 were infected with retrovirus carrying vacant or mouse PGC-1α plasmids. PGC-1α expression at the protein level was elevated with induction of its target genes ( E ). PGC-1α–dependent up-regulation ( F ) and secretion ( G ) of VEGF in MIO-M1 cells is enhanced by hypoxia. PGC-1α knockdown by shRNA inhibits VEGFA expression in normoxic and hypoxic conditions ( H ). * P

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: PGC-1α regulates VEGFA in retinal cells. RGC5 ( A ), 661W ( B ), ARPE-19 ( C ), and MIO-M1 ( D ) were infected with adenovirus expressing mouse PGC-1α or GFP (as negative control). Forty-eight hours postinfection, RNA was isolated and gene expression measured by qPCR. VEGFA was up-regulated two- to threefold in all cells. MIO-M1 were infected with retrovirus carrying vacant or mouse PGC-1α plasmids. PGC-1α expression at the protein level was elevated with induction of its target genes ( E ). PGC-1α–dependent up-regulation ( F ) and secretion ( G ) of VEGF in MIO-M1 cells is enhanced by hypoxia. PGC-1α knockdown by shRNA inhibits VEGFA expression in normoxic and hypoxic conditions ( H ). * P

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Infection, Expressing, Negative Control, Isolation, Real-time Polymerase Chain Reaction, shRNA

    PGC-1α −/− mice are protected against OIR. Oxygen-induced retinopathy (OIR) was induced by exposing PGC-1α +/+ (WT) and PGC-1α −/− mouse pups to 75% oxygen from P7 to P12. A and B : At P17, retinas were harvested and stained with isolectin-B4. The vaso-obliterated areas ( yellow outline ) and neovascular areas (marked in white) were quantified using Photoshop CS4 software. Loss of PGC-1α did not affect the avascular zone but significantly reduced the neovascular area ( n = 7 to 9). ** P

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: PGC-1α −/− mice are protected against OIR. Oxygen-induced retinopathy (OIR) was induced by exposing PGC-1α +/+ (WT) and PGC-1α −/− mouse pups to 75% oxygen from P7 to P12. A and B : At P17, retinas were harvested and stained with isolectin-B4. The vaso-obliterated areas ( yellow outline ) and neovascular areas (marked in white) were quantified using Photoshop CS4 software. Loss of PGC-1α did not affect the avascular zone but significantly reduced the neovascular area ( n = 7 to 9). ** P

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Mouse Assay, Staining, Software

    Delayed retinal vascular development and decreased capillary density in PGC-1α −/− mice. Developmental retinal vessels outgrowth was evaluated at P4 on isolectin-B4 (green) and/or collagen IV (red) stained flat-mounts ( A ) and quantified ( B ). PGC-1α −/− pups showed reduced vascular radius ( red arrows ) and area compared to WT littermates ( A and B ). High-magnification micrographs of the vascular front revealed a decreased number of tip cells ( asterisks ) in PGC-1α −/− compared to WT ( A ). Vascular density was quantified on collagen IV–stained P4 retina flat-mounts and revealed a significant decrease of capillary density in PGC-1α −/− mice ( n = 5 to 7). * P

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: Delayed retinal vascular development and decreased capillary density in PGC-1α −/− mice. Developmental retinal vessels outgrowth was evaluated at P4 on isolectin-B4 (green) and/or collagen IV (red) stained flat-mounts ( A ) and quantified ( B ). PGC-1α −/− pups showed reduced vascular radius ( red arrows ) and area compared to WT littermates ( A and B ). High-magnification micrographs of the vascular front revealed a decreased number of tip cells ( asterisks ) in PGC-1α −/− compared to WT ( A ). Vascular density was quantified on collagen IV–stained P4 retina flat-mounts and revealed a significant decrease of capillary density in PGC-1α −/− mice ( n = 5 to 7). * P

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Mouse Assay, Staining

    Region-specific regulation of PGC-1α expression during OIR. A : Schematic representation of the OIR model. B : qPCR quantification of PGC-1α and VEGFA mRNA in total retinas from P12 to P17 in control (white bars) and OIR (black bars) WT mice. C : Representative pictures of retinal sections before (Pre LCM) and after (Post LCM) laser capture microdissection. D : mRNA quantification by qPCR from laser-captured ONL, INL, and GCL of P17 control and OIR retinas (log y axis). Results are expressed as RE (relative expression) normalized to housekeeping genes (mean ± SEM, n = 3 to 4). * P

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: Region-specific regulation of PGC-1α expression during OIR. A : Schematic representation of the OIR model. B : qPCR quantification of PGC-1α and VEGFA mRNA in total retinas from P12 to P17 in control (white bars) and OIR (black bars) WT mice. C : Representative pictures of retinal sections before (Pre LCM) and after (Post LCM) laser capture microdissection. D : mRNA quantification by qPCR from laser-captured ONL, INL, and GCL of P17 control and OIR retinas (log y axis). Results are expressed as RE (relative expression) normalized to housekeeping genes (mean ± SEM, n = 3 to 4). * P

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Expressing, Real-time Polymerase Chain Reaction, Mouse Assay, Laser Capture Microdissection

    PGC-1α expression in the retina and during retinal development. A : RNA from the indicated adult murine tissues was extracted and subjected to RT-qPCR for PGC-1α. B – D : RNA from retinas of WT mice at age postnatal day 1 (P1) to P17 were harvested and subjected to RT-qPCR. The expression levels of PGC-1α ( B ) and its downstream target genes, CYCS and MCAD ( C ), were increased with development with a surge from P3 to P7. The developmental expression curve of PGC-1α also correlated with VEGFA, PDGFB, and ANG2 expression during the early stage of vascular outgrowth (up to P7). However, high PGC-1α expression is maintained during the later stage of retinal development, whereas VEGFA expression is repressed ( D ). * P

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: PGC-1α expression in the retina and during retinal development. A : RNA from the indicated adult murine tissues was extracted and subjected to RT-qPCR for PGC-1α. B – D : RNA from retinas of WT mice at age postnatal day 1 (P1) to P17 were harvested and subjected to RT-qPCR. The expression levels of PGC-1α ( B ) and its downstream target genes, CYCS and MCAD ( C ), were increased with development with a surge from P3 to P7. The developmental expression curve of PGC-1α also correlated with VEGFA, PDGFB, and ANG2 expression during the early stage of vascular outgrowth (up to P7). However, high PGC-1α expression is maintained during the later stage of retinal development, whereas VEGFA expression is repressed ( D ). * P

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Expressing, Quantitative RT-PCR, Mouse Assay

    Normal ocular and retinal structures in PGC-1α −/− mice. A and B : H E-stained sagittal ocular sections from 8-week-old PGC-1α +/+ (WT) and PGC-1α −/− mice. A : Low magnification showing normal ocular structures; scale bars = 500 μm. B : Higher magnification showing no abnormal retinal organization or layers thickness in PGC-1α −/− mice; scale bars = 50 μm. C : Representative pictograms of SD-OCT recordings from WT and PGC-1α −/− mice, the ONL is indicated by double-ended arrows . Scale bars = 100 μm. D : ONL thickness from OCT recordings were quantified every 100 μm from the optic nerve head (OD) (mean ± SD, n = 3 to 6). GCL, ganglion cell layer; INL, inner nuclear layer; IS, inner segment; ONL, outer nuclear layer; OS, outer segment.

    Journal: The American Journal of Pathology

    Article Title: PGC-1? Regulates Normal and Pathological Angiogenesis in the Retina

    doi: 10.1016/j.ajpath.2012.09.003

    Figure Lengend Snippet: Normal ocular and retinal structures in PGC-1α −/− mice. A and B : H E-stained sagittal ocular sections from 8-week-old PGC-1α +/+ (WT) and PGC-1α −/− mice. A : Low magnification showing normal ocular structures; scale bars = 500 μm. B : Higher magnification showing no abnormal retinal organization or layers thickness in PGC-1α −/− mice; scale bars = 50 μm. C : Representative pictograms of SD-OCT recordings from WT and PGC-1α −/− mice, the ONL is indicated by double-ended arrows . Scale bars = 100 μm. D : ONL thickness from OCT recordings were quantified every 100 μm from the optic nerve head (OD) (mean ± SD, n = 3 to 6). GCL, ganglion cell layer; INL, inner nuclear layer; IS, inner segment; ONL, outer nuclear layer; OS, outer segment.

    Article Snippet: PGC-1α probe was obtained by subcloning a 661-bp region of the mouse PGC-1α cDNA (nucleotides 646 to 1307) into the pBluescript KS+ vector (Stratagene/Agilent Technologies, Santa Clara, CA) and transcribed in vitro to produce antisense and sense riboprobes labeled with digoxigenin.

    Techniques: Pyrolysis Gas Chromatography, Mouse Assay, Staining