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Stratagene pfuturbo cx hotstart
Pfuturbo Cx Hotstart, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pfuturbo cx hotstart/product/Stratagene
Average 85 stars, based on 2 article reviews
Price from $9.99 to $1999.99
pfuturbo cx hotstart - by Bioz Stars, 2020-10
85/100 stars

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High Performance Liquid Chromatography:

Article Title: A one-step method for quantitative determination of uracil in DNA by real-time PCR
Article Snippet: .. Reaction mixture was in a final 10 µl volume and contained 0.05 units of PfuTurbo® Hotstart (Pfu WT-pol) or PfuTurbo® Cx Hotstart (Pfu V93Q-pol) DNA polymerase (Stratagene), 0.175 µM of each primers (Eurofins MWG Operon, HPLC grade), 200 µM of each dNTP (Fermentas), 0.5 µl EvaGreen 20× (Biotium), 30 nM Passive Reference Dye (Stratagene) and 1 µl of DNA template from one of the dilution series. .. Reactions were performed in the reaction buffer provided by the manufacturer for the PfuTurbo® Hotstart or PfuTurbo® Cx Hotstart DNA polymerases (Stratagene).

Polymerase Chain Reaction:

Article Title: UCE: A uracil excision (USER(TM))-based toolbox for transformation of cereals
Article Snippet: .. Amplification of PCR products for single-step cloning of single or multiple DNA fragments All PCR reactions of fragments to be cloned into a USER™ site was performed using the Pyrococus furiosis (Pfu) DNA polymerase PfuTurbo Cx or PfuTurbo Cx hotstart (Stratagene) in a total volume of 50 μl (5 μl Buffer supplied by the manufacturer, 5 μl dNTP 2 mM each, 5 μl forward primer, 5 μl reverse primer, 26.5 ddH2 O, 2.5 μl template plasmid DNA 1 ng/μl, 1 μl Cx DNA polymerase). .. Certain bigger fragments needed double or triple amount of DNA polymerase for PCR to work correctly.

Article Title: The patatin-like lipase family in Gallus gallus
Article Snippet: .. PCR amplification was performed with a T3000 Thermocycler (Biometra) with touch-down program using Phusion Hot Start- (Finnzymes), DyNAzyme EXT- (Finnzymes), or PfuTurbo Cx Hotstart -(Stratagene) DNA polymerase with chicken adipose tissue cDNA as template. .. In touch-down PCR, the following conditions were used during the amplification cycles: 3 cycles at annealing temperature 3°C above the melting temperature of the primer with lower value; 3 cycles at the melting temperature; 34 cycles at 3°C below the melting temperature.

Amplification:

Article Title: UCE: A uracil excision (USER(TM))-based toolbox for transformation of cereals
Article Snippet: .. Amplification of PCR products for single-step cloning of single or multiple DNA fragments All PCR reactions of fragments to be cloned into a USER™ site was performed using the Pyrococus furiosis (Pfu) DNA polymerase PfuTurbo Cx or PfuTurbo Cx hotstart (Stratagene) in a total volume of 50 μl (5 μl Buffer supplied by the manufacturer, 5 μl dNTP 2 mM each, 5 μl forward primer, 5 μl reverse primer, 26.5 ddH2 O, 2.5 μl template plasmid DNA 1 ng/μl, 1 μl Cx DNA polymerase). .. Certain bigger fragments needed double or triple amount of DNA polymerase for PCR to work correctly.

Article Title: The patatin-like lipase family in Gallus gallus
Article Snippet: .. PCR amplification was performed with a T3000 Thermocycler (Biometra) with touch-down program using Phusion Hot Start- (Finnzymes), DyNAzyme EXT- (Finnzymes), or PfuTurbo Cx Hotstart -(Stratagene) DNA polymerase with chicken adipose tissue cDNA as template. .. In touch-down PCR, the following conditions were used during the amplification cycles: 3 cycles at annealing temperature 3°C above the melting temperature of the primer with lower value; 3 cycles at the melting temperature; 34 cycles at 3°C below the melting temperature.

Plasmid Preparation:

Article Title: UCE: A uracil excision (USER(TM))-based toolbox for transformation of cereals
Article Snippet: .. Amplification of PCR products for single-step cloning of single or multiple DNA fragments All PCR reactions of fragments to be cloned into a USER™ site was performed using the Pyrococus furiosis (Pfu) DNA polymerase PfuTurbo Cx or PfuTurbo Cx hotstart (Stratagene) in a total volume of 50 μl (5 μl Buffer supplied by the manufacturer, 5 μl dNTP 2 mM each, 5 μl forward primer, 5 μl reverse primer, 26.5 ddH2 O, 2.5 μl template plasmid DNA 1 ng/μl, 1 μl Cx DNA polymerase). .. Certain bigger fragments needed double or triple amount of DNA polymerase for PCR to work correctly.

Clone Assay:

Article Title: UCE: A uracil excision (USER(TM))-based toolbox for transformation of cereals
Article Snippet: .. Amplification of PCR products for single-step cloning of single or multiple DNA fragments All PCR reactions of fragments to be cloned into a USER™ site was performed using the Pyrococus furiosis (Pfu) DNA polymerase PfuTurbo Cx or PfuTurbo Cx hotstart (Stratagene) in a total volume of 50 μl (5 μl Buffer supplied by the manufacturer, 5 μl dNTP 2 mM each, 5 μl forward primer, 5 μl reverse primer, 26.5 ddH2 O, 2.5 μl template plasmid DNA 1 ng/μl, 1 μl Cx DNA polymerase). .. Certain bigger fragments needed double or triple amount of DNA polymerase for PCR to work correctly.

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  • 85
    Stratagene pfuturbo cx hotstart dna polymerase pfucx
    Overview of the USER cloning technique. A PacI cassette containing USER vector (upper left corner) is digested with PacI and Nt.BbvCI to generate 8 nt single-stranded 3′ overhangs. A PCR fragment amplified with compatible uracil-containing primers by the <t>PfuTurbo</t> ® C x <t>Hotstart</t> <t>DNA</t> polymerase is mixed with USER™ enzyme mix (removing uracils, pink) and the linearized vector. The mixture is incubated 20 min at 37°C and 20 min at 25°C, and the hybridized product is ready to be transformed into E.coli without prior ligation. Nt.BbvCI recognition sites are marked in tan, PacI recognition sites are marked in light blue. Yellow and green mark single base differences between the generated 3′ overhangs, which are responsible for the directional insertion of the PCR fragment.
    Pfuturbo Cx Hotstart Dna Polymerase Pfucx, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfuturbo cx hotstart dna polymerase pfucx/product/Stratagene
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pfuturbo cx hotstart dna polymerase pfucx - by Bioz Stars, 2020-10
    85/100 stars
      Buy from Supplier

    90
    Stratagene pfuturbo cx hotstart dna polymerase
    Overview of the USER cloning technique. A PacI cassette containing USER vector (upper left corner) is digested with PacI and Nt.BbvCI to generate 8 nt single-stranded 3′ overhangs. A PCR fragment amplified with compatible uracil-containing primers by the <t>PfuTurbo</t> ® C x <t>Hotstart</t> <t>DNA</t> polymerase is mixed with USER™ enzyme mix (removing uracils, pink) and the linearized vector. The mixture is incubated 20 min at 37°C and 20 min at 25°C, and the hybridized product is ready to be transformed into E.coli without prior ligation. Nt.BbvCI recognition sites are marked in tan, PacI recognition sites are marked in light blue. Yellow and green mark single base differences between the generated 3′ overhangs, which are responsible for the directional insertion of the PCR fragment.
    Pfuturbo Cx Hotstart Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 90/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfuturbo cx hotstart dna polymerase/product/Stratagene
    Average 90 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    pfuturbo cx hotstart dna polymerase - by Bioz Stars, 2020-10
    90/100 stars
      Buy from Supplier

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    Overview of the USER cloning technique. A PacI cassette containing USER vector (upper left corner) is digested with PacI and Nt.BbvCI to generate 8 nt single-stranded 3′ overhangs. A PCR fragment amplified with compatible uracil-containing primers by the PfuTurbo ® C x Hotstart DNA polymerase is mixed with USER™ enzyme mix (removing uracils, pink) and the linearized vector. The mixture is incubated 20 min at 37°C and 20 min at 25°C, and the hybridized product is ready to be transformed into E.coli without prior ligation. Nt.BbvCI recognition sites are marked in tan, PacI recognition sites are marked in light blue. Yellow and green mark single base differences between the generated 3′ overhangs, which are responsible for the directional insertion of the PCR fragment.

    Journal: Nucleic Acids Research

    Article Title: Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments

    doi: 10.1093/nar/gkl635

    Figure Lengend Snippet: Overview of the USER cloning technique. A PacI cassette containing USER vector (upper left corner) is digested with PacI and Nt.BbvCI to generate 8 nt single-stranded 3′ overhangs. A PCR fragment amplified with compatible uracil-containing primers by the PfuTurbo ® C x Hotstart DNA polymerase is mixed with USER™ enzyme mix (removing uracils, pink) and the linearized vector. The mixture is incubated 20 min at 37°C and 20 min at 25°C, and the hybridized product is ready to be transformed into E.coli without prior ligation. Nt.BbvCI recognition sites are marked in tan, PacI recognition sites are marked in light blue. Yellow and green mark single base differences between the generated 3′ overhangs, which are responsible for the directional insertion of the PCR fragment.

    Article Snippet: PCR conditions PCR with the following DNA polymerases: HotMaster™ Taq DNA Polymerase (Eppendorf), Platinum® Taq DNA Polymerase (Invitrogen), Pwo DNA Polymerase (Roche), Phusion™ DNA Polymerase (Finnzymes), and PfuTurbo® Cx Hotstart DNA polymerase (PfuCx) (Stratagene) was performed according to manufacturers' instructions on pBAD-TOPO® (Invitrogen) containing the gene, At5g43440 (Accession no. AY143873).

    Techniques: Clone Assay, Plasmid Preparation, Polymerase Chain Reaction, Amplification, Incubation, Transformation Assay, Ligation, Generated

    Characteristics and applications of the USER technique. ( a ) A comparison of the ability of DNA polymerases to amplify DNA fragments using uracil-free (-dU) and uracil-containing primers (+dU). Hm Taq: HotMaster™ Taq DNA Polymerase, Taq: Platinum ® Taq DNA Polymerase, Pwo: Pwo DNA Polymerase, Phusion: Phusion™ DNA Polymerase, PfuCx: PfuTurbo ® C x Hotstart DNA polymerase. ( b ) Functional expression of A.thaliana glucose transporter AtSTP1 in X.laevis oocytes from the Xenopus -specific USER compatible vector, pNB1u. H 2 O: control oocytes injected with water; STP1: oocytes injected with AtSTP1 cRNA. ( c ) Functional expression of the cyano fluorescence protein in leaf and root of A.thaliana from the CaMV 35S promoter using the USER compatible pCAMBIA230035Su vector (35S:CFP). Control: empty pCAMBIA230035Su.

    Journal: Nucleic Acids Research

    Article Title: Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments

    doi: 10.1093/nar/gkl635

    Figure Lengend Snippet: Characteristics and applications of the USER technique. ( a ) A comparison of the ability of DNA polymerases to amplify DNA fragments using uracil-free (-dU) and uracil-containing primers (+dU). Hm Taq: HotMaster™ Taq DNA Polymerase, Taq: Platinum ® Taq DNA Polymerase, Pwo: Pwo DNA Polymerase, Phusion: Phusion™ DNA Polymerase, PfuCx: PfuTurbo ® C x Hotstart DNA polymerase. ( b ) Functional expression of A.thaliana glucose transporter AtSTP1 in X.laevis oocytes from the Xenopus -specific USER compatible vector, pNB1u. H 2 O: control oocytes injected with water; STP1: oocytes injected with AtSTP1 cRNA. ( c ) Functional expression of the cyano fluorescence protein in leaf and root of A.thaliana from the CaMV 35S promoter using the USER compatible pCAMBIA230035Su vector (35S:CFP). Control: empty pCAMBIA230035Su.

    Article Snippet: PCR conditions PCR with the following DNA polymerases: HotMaster™ Taq DNA Polymerase (Eppendorf), Platinum® Taq DNA Polymerase (Invitrogen), Pwo DNA Polymerase (Roche), Phusion™ DNA Polymerase (Finnzymes), and PfuTurbo® Cx Hotstart DNA polymerase (PfuCx) (Stratagene) was performed according to manufacturers' instructions on pBAD-TOPO® (Invitrogen) containing the gene, At5g43440 (Accession no. AY143873).

    Techniques: Functional Assay, Expressing, Plasmid Preparation, Injection, Fluorescence