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Stratagene pfuturbo cx hotstart dna polymerase
Pfuturbo Cx Hotstart Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 90/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pfuturbo cx hotstart dna polymerase/product/Stratagene
Average 90 stars, based on 29 article reviews
Price from $9.99 to $1999.99
pfuturbo cx hotstart dna polymerase - by Bioz Stars, 2020-07
90/100 stars

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Polymerase Chain Reaction:

Article Title: Hierarchical Clustering of Breast Cancer Methylomes Revealed Differentially Methylated and Expressed Breast Cancer Genes
Article Snippet: .. Four separate PCR were performed for each library using PfuTurbo Cx Hotstart DNA polymerase (Stratagene) and then pooling the enrichment products following by gel purification. .. PCR-amplified library was quantified by quantitative PCR and the library size was determined on an Agilent 2100 Bioanalyzer with High Sensitivity DNA chip.

Article Title: UCE: A uracil excision (USER(TM))-based toolbox for transformation of cereals
Article Snippet: .. According to the manufacturer the PfuTurbo Cx hotstart DNA polymerase, which is used for this purpose, has a rather low error rate (1.3 × 10-6 mutations per base per duplication) and can amplify 1 kb PCR products with 97.4% of the product error free (PfuTurbo Cx Hotstart DNA Polymerase INSTRUCTION MANUAL Catalog #600410, #600412, and #600414, Stratagene). .. This means that cloning of 5 kb amplification product will give 0.9745 ≈ 88% error free clones.

Article Title: Bisulfite sequencing of chromatin immunoprecipitated DNA (BisChIP-seq) directly informs methylation status of histone-modified DNA
Article Snippet: .. Triplicate 50-μL PCR reactions were set up as follows: 5 μL of DNA, 1.25 μL of dNTPs (10 mM) (Illumina #1000564), 1 μL of PCR primer PE 1.0 (Illumina Part #1001783), and 1 μL of PCR primer PE 2.0 (Illumina Part #1001784), in 1× PfuTurbo Cx reaction buffer (Stratagene #6000410) and 1 μL of PfuTurbo Cx Hotstart DNA polymerase (2.5 U/μL; Stratagene #600410). .. The PCR reaction was performed for 5 min at 95°C, 30 sec at 98°C, followed by 14 cycles of 10 sec at 98°C, 30 sec at 65°C, 30 sec at 72°C, for 14 cycles, then 5 min at 72°C; and hold at 4°C.

Article Title: The Genetic Basis of Constitutive and Herbivore-Induced ESP-Independent Nitrile Formation in Arabidopsis 1The Genetic Basis of Constitutive and Herbivore-Induced ESP-Independent Nitrile Formation in Arabidopsis 1 [W]The Genetic Basis of Constitutive and Herbivore-Induced ESP-Independent Nitrile Formation in Arabidopsis 1 [W] [OA]
Article Snippet: .. PCR was done in a total volume of 25 μ L of PCR buffer containing 1.25 units of PfuTurbo Cx Hotstart DNA polymerase (Stratagene), 20 to 200 ng of cDNA or 20 ng of plasmid DNA, 200 μ m dNTPs, and 10 to 20 pmol of each primer as detailed in Supplemental Table S4. .. PCR products were cloned into pET52(b)+, which had been modified and prepared for USER cloning as described ( ) and verified by sequencing.

Article Title: Replication timing and epigenome remodelling are associated with the nature of chromosomal rearrangements in cancer
Article Snippet: .. Converted libraries were enriched in five independent PCR reactions for 10 cycles using PfuTurbo Cx Hotstart DNA polymerase (Stratagene, #STG600410). .. The five independent reactions were pooled and purified using the MinElute PCR Purification Kit (QIAGEN, #28004).

Article Title: Genome-wide mapping of DNA methylation: a quantitative technology comparison
Article Snippet: .. The final bisulfite-converted DNA was eluted with 2× 20µl pre-heated (65°C) EB buffer. (vi) To determine the minimum number of PCR cycles for final library enrichment, analytical (10µl) PCR reactions containing 0.5µl of bisulfite-treated DNA, 0.2µM each of Illumina PCR primers LPX1.1 and 2.1 and 0.5U PfuTurbo Cx Hotstart DNA polymerase (Stratagene) were set up. ..

Gel Purification:

Article Title: Hierarchical Clustering of Breast Cancer Methylomes Revealed Differentially Methylated and Expressed Breast Cancer Genes
Article Snippet: .. Four separate PCR were performed for each library using PfuTurbo Cx Hotstart DNA polymerase (Stratagene) and then pooling the enrichment products following by gel purification. .. PCR-amplified library was quantified by quantitative PCR and the library size was determined on an Agilent 2100 Bioanalyzer with High Sensitivity DNA chip.

Amplification:

Article Title: Role of DNMT3B in the regulation of early neural and neural crest specifiers
Article Snippet: .. The library was then bisulfite converted using the EZ DNA Methylation-Direct kit (Zymo) according to the manufacturer's instructions and amplified for 12 cycles using 2.5 U PfuTurbo Cx Hotstart DNA Polymerase (Stratagene) and the standard amplification protocol (Illumina). .. The library's quality was assessed on a Bioanalyzer (Agilent) and sequenced (100 bp, single-ended) on an Illumina HiSeq 2000.

Plasmid Preparation:

Article Title: The Genetic Basis of Constitutive and Herbivore-Induced ESP-Independent Nitrile Formation in Arabidopsis 1The Genetic Basis of Constitutive and Herbivore-Induced ESP-Independent Nitrile Formation in Arabidopsis 1 [W]The Genetic Basis of Constitutive and Herbivore-Induced ESP-Independent Nitrile Formation in Arabidopsis 1 [W] [OA]
Article Snippet: .. PCR was done in a total volume of 25 μ L of PCR buffer containing 1.25 units of PfuTurbo Cx Hotstart DNA polymerase (Stratagene), 20 to 200 ng of cDNA or 20 ng of plasmid DNA, 200 μ m dNTPs, and 10 to 20 pmol of each primer as detailed in Supplemental Table S4. .. PCR products were cloned into pET52(b)+, which had been modified and prepared for USER cloning as described ( ) and verified by sequencing.

Methylation:

Article Title: Role of DNMT3B in the regulation of early neural and neural crest specifiers
Article Snippet: .. The library was then bisulfite converted using the EZ DNA Methylation-Direct kit (Zymo) according to the manufacturer's instructions and amplified for 12 cycles using 2.5 U PfuTurbo Cx Hotstart DNA Polymerase (Stratagene) and the standard amplification protocol (Illumina). .. The library's quality was assessed on a Bioanalyzer (Agilent) and sequenced (100 bp, single-ended) on an Illumina HiSeq 2000.

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    Stratagene pfuturbo cx hotstart dna polymerase pfucx
    Overview of the USER cloning technique. A PacI cassette containing USER vector (upper left corner) is digested with PacI and Nt.BbvCI to generate 8 nt single-stranded 3′ overhangs. A PCR fragment amplified with compatible uracil-containing primers by the <t>PfuTurbo</t> ® C x <t>Hotstart</t> <t>DNA</t> polymerase is mixed with USER™ enzyme mix (removing uracils, pink) and the linearized vector. The mixture is incubated 20 min at 37°C and 20 min at 25°C, and the hybridized product is ready to be transformed into E.coli without prior ligation. Nt.BbvCI recognition sites are marked in tan, PacI recognition sites are marked in light blue. Yellow and green mark single base differences between the generated 3′ overhangs, which are responsible for the directional insertion of the PCR fragment.
    Pfuturbo Cx Hotstart Dna Polymerase Pfucx, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfuturbo cx hotstart dna polymerase pfucx/product/Stratagene
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pfuturbo cx hotstart dna polymerase pfucx - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    90
    Stratagene pfuturbo cx hotstart dna polymerase
    Overview of the USER cloning technique. A PacI cassette containing USER vector (upper left corner) is digested with PacI and Nt.BbvCI to generate 8 nt single-stranded 3′ overhangs. A PCR fragment amplified with compatible uracil-containing primers by the <t>PfuTurbo</t> ® C x <t>Hotstart</t> <t>DNA</t> polymerase is mixed with USER™ enzyme mix (removing uracils, pink) and the linearized vector. The mixture is incubated 20 min at 37°C and 20 min at 25°C, and the hybridized product is ready to be transformed into E.coli without prior ligation. Nt.BbvCI recognition sites are marked in tan, PacI recognition sites are marked in light blue. Yellow and green mark single base differences between the generated 3′ overhangs, which are responsible for the directional insertion of the PCR fragment.
    Pfuturbo Cx Hotstart Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 90/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfuturbo cx hotstart dna polymerase/product/Stratagene
    Average 90 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    pfuturbo cx hotstart dna polymerase - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

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    Overview of the USER cloning technique. A PacI cassette containing USER vector (upper left corner) is digested with PacI and Nt.BbvCI to generate 8 nt single-stranded 3′ overhangs. A PCR fragment amplified with compatible uracil-containing primers by the PfuTurbo ® C x Hotstart DNA polymerase is mixed with USER™ enzyme mix (removing uracils, pink) and the linearized vector. The mixture is incubated 20 min at 37°C and 20 min at 25°C, and the hybridized product is ready to be transformed into E.coli without prior ligation. Nt.BbvCI recognition sites are marked in tan, PacI recognition sites are marked in light blue. Yellow and green mark single base differences between the generated 3′ overhangs, which are responsible for the directional insertion of the PCR fragment.

    Journal: Nucleic Acids Research

    Article Title: Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments

    doi: 10.1093/nar/gkl635

    Figure Lengend Snippet: Overview of the USER cloning technique. A PacI cassette containing USER vector (upper left corner) is digested with PacI and Nt.BbvCI to generate 8 nt single-stranded 3′ overhangs. A PCR fragment amplified with compatible uracil-containing primers by the PfuTurbo ® C x Hotstart DNA polymerase is mixed with USER™ enzyme mix (removing uracils, pink) and the linearized vector. The mixture is incubated 20 min at 37°C and 20 min at 25°C, and the hybridized product is ready to be transformed into E.coli without prior ligation. Nt.BbvCI recognition sites are marked in tan, PacI recognition sites are marked in light blue. Yellow and green mark single base differences between the generated 3′ overhangs, which are responsible for the directional insertion of the PCR fragment.

    Article Snippet: PCR conditions PCR with the following DNA polymerases: HotMaster™ Taq DNA Polymerase (Eppendorf), Platinum® Taq DNA Polymerase (Invitrogen), Pwo DNA Polymerase (Roche), Phusion™ DNA Polymerase (Finnzymes), and PfuTurbo® Cx Hotstart DNA polymerase (PfuCx) (Stratagene) was performed according to manufacturers' instructions on pBAD-TOPO® (Invitrogen) containing the gene, At5g43440 (Accession no. AY143873).

    Techniques: Clone Assay, Plasmid Preparation, Polymerase Chain Reaction, Amplification, Incubation, Transformation Assay, Ligation, Generated

    Characteristics and applications of the USER technique. ( a ) A comparison of the ability of DNA polymerases to amplify DNA fragments using uracil-free (-dU) and uracil-containing primers (+dU). Hm Taq: HotMaster™ Taq DNA Polymerase, Taq: Platinum ® Taq DNA Polymerase, Pwo: Pwo DNA Polymerase, Phusion: Phusion™ DNA Polymerase, PfuCx: PfuTurbo ® C x Hotstart DNA polymerase. ( b ) Functional expression of A.thaliana glucose transporter AtSTP1 in X.laevis oocytes from the Xenopus -specific USER compatible vector, pNB1u. H 2 O: control oocytes injected with water; STP1: oocytes injected with AtSTP1 cRNA. ( c ) Functional expression of the cyano fluorescence protein in leaf and root of A.thaliana from the CaMV 35S promoter using the USER compatible pCAMBIA230035Su vector (35S:CFP). Control: empty pCAMBIA230035Su.

    Journal: Nucleic Acids Research

    Article Title: Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments

    doi: 10.1093/nar/gkl635

    Figure Lengend Snippet: Characteristics and applications of the USER technique. ( a ) A comparison of the ability of DNA polymerases to amplify DNA fragments using uracil-free (-dU) and uracil-containing primers (+dU). Hm Taq: HotMaster™ Taq DNA Polymerase, Taq: Platinum ® Taq DNA Polymerase, Pwo: Pwo DNA Polymerase, Phusion: Phusion™ DNA Polymerase, PfuCx: PfuTurbo ® C x Hotstart DNA polymerase. ( b ) Functional expression of A.thaliana glucose transporter AtSTP1 in X.laevis oocytes from the Xenopus -specific USER compatible vector, pNB1u. H 2 O: control oocytes injected with water; STP1: oocytes injected with AtSTP1 cRNA. ( c ) Functional expression of the cyano fluorescence protein in leaf and root of A.thaliana from the CaMV 35S promoter using the USER compatible pCAMBIA230035Su vector (35S:CFP). Control: empty pCAMBIA230035Su.

    Article Snippet: PCR conditions PCR with the following DNA polymerases: HotMaster™ Taq DNA Polymerase (Eppendorf), Platinum® Taq DNA Polymerase (Invitrogen), Pwo DNA Polymerase (Roche), Phusion™ DNA Polymerase (Finnzymes), and PfuTurbo® Cx Hotstart DNA polymerase (PfuCx) (Stratagene) was performed according to manufacturers' instructions on pBAD-TOPO® (Invitrogen) containing the gene, At5g43440 (Accession no. AY143873).

    Techniques: Functional Assay, Expressing, Plasmid Preparation, Injection, Fluorescence