pfu polymerase  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Thermo Fisher pfu polymerase
    Pfu Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfu polymerase/product/Thermo Fisher
    Average 93 stars, based on 143 article reviews
    Price from $9.99 to $1999.99
    pfu polymerase - by Bioz Stars, 2020-05
    93/100 stars

    Images

    Related Articles

    other:

    Article Title:
    Article Snippet: Mutations in a transgene were detected by PCR cloning using Pfu polymerase (Agilent Technologies), and eGFP primers Fwd2 (5′-TTTCTGCTGCTGCTTGCGTACGG-3′) and Rev6 (5′-GCCGTTCTTC­TGCTTGTCGGC­CATGATATAG-3′) were used for PCR cloning with Pfu polymerase at 95°C for 30 s, 58°C for 30 s, and 72°C for 90 s for 24 cycles and cloned into a PCR cloning kit (Zero Blunt TOPO; Invitrogen).

    Article Title:
    Article Snippet: Full-length recombinant C. muridarum IncA ( ) and CPAF ( ) were produced by amplifying full-length coding sequences with primers ( ) using Pfu polymerase (Promega, Alexandria, NSW, Australia) and hotstart PCR conditions of 95 °C for 2 min, addition of Pfu polymerase, then 35 cycles of 95 °C for 1 min, 60 °C for 1 min and 74 °C for 5 min. Amplicons were purified using Purelink PCR purification columns (Invitrogen) and restriction digested with BamHI/EcoRI (IncA) or BamHI/KpnI (CPAF) for 1 h at 37 °C.

    Article Title:
    Article Snippet: Mutations in knock-in clones were detected by PCR cloning using Pfu polymerase (Agilent Technologies), and primers pk64 and pk10 (Table S1) were used for PCR cloning with Pfu polymerase at 95°C for 30 s, 67°C for 30 s, and 72°C for 160 s for 25 cycles, and cloned with a PCR cloning kit (Zero Blunt TOPO; Invitrogen).

    Article Title:
    Article Snippet: Mutations in knock-in clones were detected by PCR cloning using Pfu polymerase (Agilent Technologies), and primers PK64 and PK65 (see Table S1 in the supplemental material) were used for PCR cloning with Pfu polymerase at 95°C for 30 s, 67°C for 30 s, and 72°C for 160 s for 25 cycles and cloning in a PCR cloning kit (Zero Blunt Topo; Invitrogen).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher pfu dna polymerase
    Results of ‘RNA-induced <t>DNA</t> replication interference’ assays. a Effects of 27nt-RNAs using <t>Pfu</t> or Taq polymerases after end-point-PCR and agarose gel electrophoresis (top) or after qPCR (bottom). b Effects of 27nt-RNAs alone or in combination with PIWI1 on linear DNA amplification in a Klenow reaction were assayed via qPCR. c Hypothetical models on sequence-specific targeting through Argonaute/PIWI-RNA complexes (blue: IES, red: MDS, yellow: PIWI1, green: 27nt-RNA, orange: tethered transcript [ c1 only]): c1 According to the ‚nascent transcript model, 27nt-RNA/PIWI1 complexes could target tethered IES-originating transcripts, which would be reminiscent of observations made in divergent eukaryotes, such as S. pombe , C. elegans and A. thaliana ]). Alternatively, we assumed that 27nt-RNA/PIWI1 complexes could interact with dsDNA ( c2 ) or via base-pairing with ssDNA, possibly when it occurs in a replication bubble ( c3 ). d Mapping of mRNA reads purified 20 h PC on micronuclear model genes reveals that IES (red bars) are sharply omitted
    Pfu Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 542 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfu dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 542 article reviews
    Price from $9.99 to $1999.99
    pfu dna polymerase - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    Results of ‘RNA-induced DNA replication interference’ assays. a Effects of 27nt-RNAs using Pfu or Taq polymerases after end-point-PCR and agarose gel electrophoresis (top) or after qPCR (bottom). b Effects of 27nt-RNAs alone or in combination with PIWI1 on linear DNA amplification in a Klenow reaction were assayed via qPCR. c Hypothetical models on sequence-specific targeting through Argonaute/PIWI-RNA complexes (blue: IES, red: MDS, yellow: PIWI1, green: 27nt-RNA, orange: tethered transcript [ c1 only]): c1 According to the ‚nascent transcript model, 27nt-RNA/PIWI1 complexes could target tethered IES-originating transcripts, which would be reminiscent of observations made in divergent eukaryotes, such as S. pombe , C. elegans and A. thaliana ]). Alternatively, we assumed that 27nt-RNA/PIWI1 complexes could interact with dsDNA ( c2 ) or via base-pairing with ssDNA, possibly when it occurs in a replication bubble ( c3 ). d Mapping of mRNA reads purified 20 h PC on micronuclear model genes reveals that IES (red bars) are sharply omitted

    Journal: Epigenetics & Chromatin

    Article Title: 27nt-RNAs guide histone variant deposition via ‘RNA-induced DNA replication interference’ and thus transmit parental genome partitioning in Stylonychia

    doi: 10.1186/s13072-018-0201-5

    Figure Lengend Snippet: Results of ‘RNA-induced DNA replication interference’ assays. a Effects of 27nt-RNAs using Pfu or Taq polymerases after end-point-PCR and agarose gel electrophoresis (top) or after qPCR (bottom). b Effects of 27nt-RNAs alone or in combination with PIWI1 on linear DNA amplification in a Klenow reaction were assayed via qPCR. c Hypothetical models on sequence-specific targeting through Argonaute/PIWI-RNA complexes (blue: IES, red: MDS, yellow: PIWI1, green: 27nt-RNA, orange: tethered transcript [ c1 only]): c1 According to the ‚nascent transcript model, 27nt-RNA/PIWI1 complexes could target tethered IES-originating transcripts, which would be reminiscent of observations made in divergent eukaryotes, such as S. pombe , C. elegans and A. thaliana ]). Alternatively, we assumed that 27nt-RNA/PIWI1 complexes could interact with dsDNA ( c2 ) or via base-pairing with ssDNA, possibly when it occurs in a replication bubble ( c3 ). d Mapping of mRNA reads purified 20 h PC on micronuclear model genes reveals that IES (red bars) are sharply omitted

    Article Snippet: Standard PCR conditions were as follows: 95 °C for 10 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 30 s], 72 °C for 10 min; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 45 s], 72 °C for 10 min. Quantitative PCR was performed on a Rotor Gene 6000 (Corbett Life Science) using QuantiTect SYBR green Taq polymerase Master Mix (Qiagen) or Pfu DNA polymerase (ThermoFisher) and SYBR green (Sigma-Aldrich).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Real-time Polymerase Chain Reaction, Amplification, Sequencing, Purification

    Cloning and sub-cloning of CD19 cDNA. A) Amplification of specific band for human CD19 cDNA using Pfu DNA polymerase; B) Colony-PCR reaction on eight white colonies (1-8) after blue/ white selection. C) Excision of 1701 bp band for human CD19 cDNA after double digestion of the construct using KpnI and HindIII restriction enzymes. Lanes (a) and (a’): undigested pGEMT-easy/CD19 construct, Lanes (b) partial and complete digestion by KpnI and Hind III, respectively and (b’): complete digestion by both KpnI and HindIII. SM: DNA size marker ( bp ). Asterisks (*) point the desired band.

    Journal: Avicenna Journal of Medical Biotechnology

    Article Title: Cloning and Expression of CD19, a Human B-Cell Marker in NIH-3T3 Cell Line

    doi:

    Figure Lengend Snippet: Cloning and sub-cloning of CD19 cDNA. A) Amplification of specific band for human CD19 cDNA using Pfu DNA polymerase; B) Colony-PCR reaction on eight white colonies (1-8) after blue/ white selection. C) Excision of 1701 bp band for human CD19 cDNA after double digestion of the construct using KpnI and HindIII restriction enzymes. Lanes (a) and (a’): undigested pGEMT-easy/CD19 construct, Lanes (b) partial and complete digestion by KpnI and Hind III, respectively and (b’): complete digestion by both KpnI and HindIII. SM: DNA size marker ( bp ). Asterisks (*) point the desired band.

    Article Snippet: For amplification of CD19 cDNA, PCR was performed in a 25 µl reaction solution contained 2.5 µl 10×PCR buffer, 1.5 µl 10 mM dNTPs, 1 µl of each primer (10 pmol/µl ), 0.5 µl Pfu DNA polymerase (10 U/µl ) (Thermo Fisher Scientific, Inc., MA, USA), 1 mM MgSO4 and 1 µl cDNA.

    Techniques: Clone Assay, Subcloning, Amplification, Polymerase Chain Reaction, Selection, Construct, Marker

    Results of ‘RNA-induced DNA replication interference’ assays. a Effects of 27nt-RNAs using Pfu or Taq polymerases after end-point-PCR and agarose gel electropho resis (top) or after qPCR (bottom). b Effects of 27nt-RNAs alone or in combination with PIWI1 on linear DNA amplification in a Klenow reaction were assayed via qPCR. c Hypothetical models on sequence-specific targeting through Argonaute/PIWI-RNA complexes (blue: IES, red: MDS, yellow: PIWI1, green: 27nt-RNA, orange: tethered transcript [ c1 only]): c1 According to the ‚nascent transcript model, 27nt-RNA/PIWI1 complexes could target tethered IES-originating transcripts, which would be reminiscent of observations made in divergent eukaryotes, such as S. pombe , C. elegans and A. thaliana (reviewed in [ 38 ]). Alternatively, we assumed that 27nt-RNA/PIWI1 complexes could interact with dsDNA ( c2 ) or via base-pairing with ssDNA, possibly when it occurs in a replication bubble ( c3 ). d Mapping of mRNA reads purified 20 h PC on micronuclear model genes reveals that IES (red bars) are sharply omitted

    Journal: Epigenetics & Chromatin

    Article Title: 27nt-RNAs guide histone variant deposition via ‘RNA-induced DNA replication interference’ and thus transmit parental genome partitioning in Stylonychia

    doi: 10.1186/s13072-018-0201-5

    Figure Lengend Snippet: Results of ‘RNA-induced DNA replication interference’ assays. a Effects of 27nt-RNAs using Pfu or Taq polymerases after end-point-PCR and agarose gel electropho resis (top) or after qPCR (bottom). b Effects of 27nt-RNAs alone or in combination with PIWI1 on linear DNA amplification in a Klenow reaction were assayed via qPCR. c Hypothetical models on sequence-specific targeting through Argonaute/PIWI-RNA complexes (blue: IES, red: MDS, yellow: PIWI1, green: 27nt-RNA, orange: tethered transcript [ c1 only]): c1 According to the ‚nascent transcript model, 27nt-RNA/PIWI1 complexes could target tethered IES-originating transcripts, which would be reminiscent of observations made in divergent eukaryotes, such as S. pombe , C. elegans and A. thaliana (reviewed in [ 38 ]). Alternatively, we assumed that 27nt-RNA/PIWI1 complexes could interact with dsDNA ( c2 ) or via base-pairing with ssDNA, possibly when it occurs in a replication bubble ( c3 ). d Mapping of mRNA reads purified 20 h PC on micronuclear model genes reveals that IES (red bars) are sharply omitted

    Article Snippet: Standard PCR conditions were as follows: 95 °C for 10 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 30 s], 72 °C for 10 min; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 45 s], 72 °C for 10 min. Quantitative PCR was performed on a Rotor Gene 6000 (Corbett Life Science) using QuantiTect SYBR green Taq polymerase Master Mix (Qiagen) or Pfu DNA polymerase (ThermoFisher) and SYBR green (Sigma-Aldrich).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Real-time Polymerase Chain Reaction, Amplification, Sequencing, Purification