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Promega pfu dnapol
Pfu Dnapol, supplied by Promega, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pfu dnapol/product/Promega
Average 87 stars, based on 1 article reviews
Price from $9.99 to $1999.99
pfu dnapol - by Bioz Stars, 2020-04
87/100 stars

Related Products / Commonly Used Together

fix7 bac
extension pcr
hcmv ul55 orf
c-terminal twin-strep-tag

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Related Articles

BAC Assay:

Article Title: Enhanced Expression of Full-Length Human Cytomegalovirus Fusion Protein in Non-Swelling Baculovirus-Infected Cells with a Minimal Fed-Batch Strategy
Article Snippet: .. Primers listed in have been used to amplify the HCMV UL55 ORF from FIX7 BAC , replacing the wt stop codon with an in-frame 93 bp-long sequence coding for a C-terminal Twin-Strep-tag® by 5′ extension PCR with Pfu DNApol (Promega). .. The DNA sequence coding for the tag was manually adapted based on Gene Designer 2.0 software (DNA 2.0 Inc.).

Infection:

Article Title: Enhanced Expression of Full-Length Human Cytomegalovirus Fusion Protein in Non-Swelling Baculovirus-Infected Cells with a Minimal Fed-Batch Strategy
Article Snippet: Rocking WAVE 20/50EHT Bioreactor™ (50 L bag for 25 L culture volume, GE Healthcare) was used for up-scaling FL-gB production in High Five and operated via UBICON at 10 to 22 rpm with 7° rocking angle and 0.1 to 0.3 Liter·min−1 aeration rate; 300x concentrated cysteine feed was prepared in culture medium and provided in a continuous fashion through a gravimetric controlled pump at 100 mL·day−1 starting at 24 hours after the infection. .. Primers listed in have been used to amplify the HCMV UL55 ORF from FIX7 BAC , replacing the wt stop codon with an in-frame 93 bp-long sequence coding for a C-terminal Twin-Strep-tag® by 5′ extension PCR with Pfu DNApol (Promega).

Sequencing:

Article Title: Enhanced Expression of Full-Length Human Cytomegalovirus Fusion Protein in Non-Swelling Baculovirus-Infected Cells with a Minimal Fed-Batch Strategy
Article Snippet: .. Primers listed in have been used to amplify the HCMV UL55 ORF from FIX7 BAC , replacing the wt stop codon with an in-frame 93 bp-long sequence coding for a C-terminal Twin-Strep-tag® by 5′ extension PCR with Pfu DNApol (Promega). .. The DNA sequence coding for the tag was manually adapted based on Gene Designer 2.0 software (DNA 2.0 Inc.).

Generated:

Article Title: Enhanced Expression of Full-Length Human Cytomegalovirus Fusion Protein in Non-Swelling Baculovirus-Infected Cells with a Minimal Fed-Batch Strategy
Article Snippet: Primers listed in have been used to amplify the HCMV UL55 ORF from FIX7 BAC , replacing the wt stop codon with an in-frame 93 bp-long sequence coding for a C-terminal Twin-Strep-tag® by 5′ extension PCR with Pfu DNApol (Promega). .. After sequence validation of the resulting UL55strep2x, recombinant Ac MNPV expressing Strep-tagged FL-gB under polh promoter (termed bBst2x) was generated by Tn7-mediated transposition according to Bac-to-Bac® system guidelines (Life Technologies).

Expressing:

Article Title: Enhanced Expression of Full-Length Human Cytomegalovirus Fusion Protein in Non-Swelling Baculovirus-Infected Cells with a Minimal Fed-Batch Strategy
Article Snippet: Primers listed in have been used to amplify the HCMV UL55 ORF from FIX7 BAC , replacing the wt stop codon with an in-frame 93 bp-long sequence coding for a C-terminal Twin-Strep-tag® by 5′ extension PCR with Pfu DNApol (Promega). .. After sequence validation of the resulting UL55strep2x, recombinant Ac MNPV expressing Strep-tagged FL-gB under polh promoter (termed bBst2x) was generated by Tn7-mediated transposition according to Bac-to-Bac® system guidelines (Life Technologies).

Polymerase Chain Reaction:

Article Title: Enhanced Expression of Full-Length Human Cytomegalovirus Fusion Protein in Non-Swelling Baculovirus-Infected Cells with a Minimal Fed-Batch Strategy
Article Snippet: .. Primers listed in have been used to amplify the HCMV UL55 ORF from FIX7 BAC , replacing the wt stop codon with an in-frame 93 bp-long sequence coding for a C-terminal Twin-Strep-tag® by 5′ extension PCR with Pfu DNApol (Promega). .. The DNA sequence coding for the tag was manually adapted based on Gene Designer 2.0 software (DNA 2.0 Inc.).

Recombinant:

Article Title: Enhanced Expression of Full-Length Human Cytomegalovirus Fusion Protein in Non-Swelling Baculovirus-Infected Cells with a Minimal Fed-Batch Strategy
Article Snippet: Paragraph title: Cell Cultures and Recombinant Baculovirus ... Primers listed in have been used to amplify the HCMV UL55 ORF from FIX7 BAC , replacing the wt stop codon with an in-frame 93 bp-long sequence coding for a C-terminal Twin-Strep-tag® by 5′ extension PCR with Pfu DNApol (Promega).

Software:

Article Title: Enhanced Expression of Full-Length Human Cytomegalovirus Fusion Protein in Non-Swelling Baculovirus-Infected Cells with a Minimal Fed-Batch Strategy
Article Snippet: Primers listed in have been used to amplify the HCMV UL55 ORF from FIX7 BAC , replacing the wt stop codon with an in-frame 93 bp-long sequence coding for a C-terminal Twin-Strep-tag® by 5′ extension PCR with Pfu DNApol (Promega). .. The DNA sequence coding for the tag was manually adapted based on Gene Designer 2.0 software (DNA 2.0 Inc.).

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  • 87
    Promega pfu dna polymerase amplified pcr
    FoxO6 directly binds to an upstream A/T-rich element in the PGC-1 α promoter ( A and B ) Bacteria expressed MBP or MBP-FoxO6 (0.5 μg) was used to bind 32 P-labelled <t>DNA</t> probes spanning the region −2252 to −1843 in EMSA. Cold probe and Gapdh DNA (×100) were included in the reaction to test the binding specificity of MBP-FoxO6 to each probe. ( C ) DNase I footprinting of MBP-FoxO6-binding sites in PGC-1 α promoter. 32 P-labelled DNA probes (−2252 to 1843) was digested with DNAse I in the presence and absence of MBP or MBP-FoxO6 proteins before resolved on a 6% sequencing gel. The signals on the gel were viewed by autoradiography. The corresponding position of each base in the probe is shown to the left. The images of both short (16 h, left panel) and long (48 h, right panel) exposures of a representative gel are shown here. ( D ) CHIP assay using antibodies against FoxO6 or non-specific IgG. Precipitated chromatin was amplified by primer sets targeting different regions of the PGC-1 α promoter. The priming sites are shown on the top panel. NTC, no template control for <t>PCR.</t> The upstream priming sites (−99875 to −99642) were used as a negative control of CHIP assay.
    Pfu Dna Polymerase Amplified Pcr, supplied by Promega, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfu dna polymerase amplified pcr/product/Promega
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pfu dna polymerase amplified pcr - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    88
    Promega proof reading pfu dna polymerase
    CEBPA binds to and activates the HK3 and the KLF5 promoters. Schematic representation of a 6 kb human HK3 (a) and a 5 kb human KLF5 (c) genomic regions retrieved from an online database. MatInspector software predicted two putative CEBPA binding sites (squares) in the <t>DNA</t> sequences analyzed. In vivo binding of CEBPA to these CEBPA consensus sites in the HK3 (b) or KLF5 (d) genomic regions was shown by chromatin immunoprecipitation (ChIP) in NB4 APL cells. As a negative control for the different pull downs, absence of GAPDH amplification is shown. *unspecific band, primer dimer. Two HK3 (e–f) and one KLF5 genomic region (g) containing the CEBPA binding sites were PCR amplified from genomic DNA of NB4 cells using proof reading <t>Pfu</t> DNA polymerase and cloned into the pGL4.10-basic vector. H1299 cells were transiently transfected with 40 ng of either HK3 promoter reporter construct A (e), construct A with mutated CEBPA binding site (f, wild-type GAAAGAC, mutated GGTCGAC) or the KLF5 promoter reporter construct (g), together with pcDNA3.1 empty vector or increasing concentrations (40–80–120 ng) (e,g) or 80 ng of CEBPA expression vector (f). The promoter activity is shown as relative light units (RLU) relative to pcDNA3.1 control transfected cells. Results are the means ± s.d. of at least triplicate transfections. MWU: **p
    Proof Reading Pfu Dna Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 469 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proof reading pfu dna polymerase/product/Promega
    Average 88 stars, based on 469 article reviews
    Price from $9.99 to $1999.99
    proof reading pfu dna polymerase - by Bioz Stars, 2020-04
    88/100 stars
      Buy from Supplier

    Image Search Results


    FoxO6 directly binds to an upstream A/T-rich element in the PGC-1 α promoter ( A and B ) Bacteria expressed MBP or MBP-FoxO6 (0.5 μg) was used to bind 32 P-labelled DNA probes spanning the region −2252 to −1843 in EMSA. Cold probe and Gapdh DNA (×100) were included in the reaction to test the binding specificity of MBP-FoxO6 to each probe. ( C ) DNase I footprinting of MBP-FoxO6-binding sites in PGC-1 α promoter. 32 P-labelled DNA probes (−2252 to 1843) was digested with DNAse I in the presence and absence of MBP or MBP-FoxO6 proteins before resolved on a 6% sequencing gel. The signals on the gel were viewed by autoradiography. The corresponding position of each base in the probe is shown to the left. The images of both short (16 h, left panel) and long (48 h, right panel) exposures of a representative gel are shown here. ( D ) CHIP assay using antibodies against FoxO6 or non-specific IgG. Precipitated chromatin was amplified by primer sets targeting different regions of the PGC-1 α promoter. The priming sites are shown on the top panel. NTC, no template control for PCR. The upstream priming sites (−99875 to −99642) were used as a negative control of CHIP assay.

    Journal: Bioscience Reports

    Article Title: FoxO6 and PGC-1? form a regulatory loop in myogenic cells

    doi: 10.1042/BSR20130031

    Figure Lengend Snippet: FoxO6 directly binds to an upstream A/T-rich element in the PGC-1 α promoter ( A and B ) Bacteria expressed MBP or MBP-FoxO6 (0.5 μg) was used to bind 32 P-labelled DNA probes spanning the region −2252 to −1843 in EMSA. Cold probe and Gapdh DNA (×100) were included in the reaction to test the binding specificity of MBP-FoxO6 to each probe. ( C ) DNase I footprinting of MBP-FoxO6-binding sites in PGC-1 α promoter. 32 P-labelled DNA probes (−2252 to 1843) was digested with DNAse I in the presence and absence of MBP or MBP-FoxO6 proteins before resolved on a 6% sequencing gel. The signals on the gel were viewed by autoradiography. The corresponding position of each base in the probe is shown to the left. The images of both short (16 h, left panel) and long (48 h, right panel) exposures of a representative gel are shown here. ( D ) CHIP assay using antibodies against FoxO6 or non-specific IgG. Precipitated chromatin was amplified by primer sets targeting different regions of the PGC-1 α promoter. The priming sites are shown on the top panel. NTC, no template control for PCR. The upstream priming sites (−99875 to −99642) were used as a negative control of CHIP assay.

    Article Snippet: Reporters containing other regions of the PGC-1 α promoter were created by inserting pfu DNA polymerase-amplified PCR products into the SmaI site of pGL2-tk-enhancer vector (Promega).

    Techniques: Pyrolysis Gas Chromatography, Binding Assay, Footprinting, Sequencing, Autoradiography, Chromatin Immunoprecipitation, Amplification, Polymerase Chain Reaction, Negative Control

    CEBPA binds to and activates the HK3 and the KLF5 promoters. Schematic representation of a 6 kb human HK3 (a) and a 5 kb human KLF5 (c) genomic regions retrieved from an online database. MatInspector software predicted two putative CEBPA binding sites (squares) in the DNA sequences analyzed. In vivo binding of CEBPA to these CEBPA consensus sites in the HK3 (b) or KLF5 (d) genomic regions was shown by chromatin immunoprecipitation (ChIP) in NB4 APL cells. As a negative control for the different pull downs, absence of GAPDH amplification is shown. *unspecific band, primer dimer. Two HK3 (e–f) and one KLF5 genomic region (g) containing the CEBPA binding sites were PCR amplified from genomic DNA of NB4 cells using proof reading Pfu DNA polymerase and cloned into the pGL4.10-basic vector. H1299 cells were transiently transfected with 40 ng of either HK3 promoter reporter construct A (e), construct A with mutated CEBPA binding site (f, wild-type GAAAGAC, mutated GGTCGAC) or the KLF5 promoter reporter construct (g), together with pcDNA3.1 empty vector or increasing concentrations (40–80–120 ng) (e,g) or 80 ng of CEBPA expression vector (f). The promoter activity is shown as relative light units (RLU) relative to pcDNA3.1 control transfected cells. Results are the means ± s.d. of at least triplicate transfections. MWU: **p

    Journal: Scientific Reports

    Article Title: CEBPA-dependent HK3 and KLF5 expression in primary AML and during AML differentiation

    doi: 10.1038/srep04261

    Figure Lengend Snippet: CEBPA binds to and activates the HK3 and the KLF5 promoters. Schematic representation of a 6 kb human HK3 (a) and a 5 kb human KLF5 (c) genomic regions retrieved from an online database. MatInspector software predicted two putative CEBPA binding sites (squares) in the DNA sequences analyzed. In vivo binding of CEBPA to these CEBPA consensus sites in the HK3 (b) or KLF5 (d) genomic regions was shown by chromatin immunoprecipitation (ChIP) in NB4 APL cells. As a negative control for the different pull downs, absence of GAPDH amplification is shown. *unspecific band, primer dimer. Two HK3 (e–f) and one KLF5 genomic region (g) containing the CEBPA binding sites were PCR amplified from genomic DNA of NB4 cells using proof reading Pfu DNA polymerase and cloned into the pGL4.10-basic vector. H1299 cells were transiently transfected with 40 ng of either HK3 promoter reporter construct A (e), construct A with mutated CEBPA binding site (f, wild-type GAAAGAC, mutated GGTCGAC) or the KLF5 promoter reporter construct (g), together with pcDNA3.1 empty vector or increasing concentrations (40–80–120 ng) (e,g) or 80 ng of CEBPA expression vector (f). The promoter activity is shown as relative light units (RLU) relative to pcDNA3.1 control transfected cells. Results are the means ± s.d. of at least triplicate transfections. MWU: **p

    Article Snippet: Human HK3 and KLF5 promoter reporter assays and mutagenesis Two HK3 promoter regions and one KLF5 promoter region containing the CEBPA binding sites were PCR amplified from genomic DNA of NB4 APL cells using proof reading Pfu DNA polymerase (Promega, Dübendorf, Switzerland) and cloned into the pGL4.10-basic vector (Promega).

    Techniques: Software, Binding Assay, In Vivo, Chromatin Immunoprecipitation, Negative Control, Amplification, Polymerase Chain Reaction, Clone Assay, Plasmid Preparation, Transfection, Construct, Expressing, Activity Assay

    (a) Schematic presentation of the bipolar pyrimidine operon. The approximate position of oligonucleotides used as primers in the RT-PCRs and their polarity are indicated by small arrows. (b, c, d, and e) Analysis by agarose gel electrophoresis of double-stranded DNA fragments generated in the RT-PCRs performed with S. acidocaldarius total RNA, Expand reverse transcriptase (when indicated), Pfu DNA polymerase, and different combinations of primers, as indicated. MW, molecular size markers.

    Journal: Journal of Bacteriology

    Article Title: Genes of De Novo Pyrimidine Biosynthesis from the Hyperthermoacidophilic Crenarchaeote Sulfolobus acidocaldarius: Novel Organization in a Bipolar Operon

    doi: 10.1128/JB.184.16.4430-4441.2002

    Figure Lengend Snippet: (a) Schematic presentation of the bipolar pyrimidine operon. The approximate position of oligonucleotides used as primers in the RT-PCRs and their polarity are indicated by small arrows. (b, c, d, and e) Analysis by agarose gel electrophoresis of double-stranded DNA fragments generated in the RT-PCRs performed with S. acidocaldarius total RNA, Expand reverse transcriptase (when indicated), Pfu DNA polymerase, and different combinations of primers, as indicated. MW, molecular size markers.

    Article Snippet: Three-microliter cDNA aliquots were used as a template in the PCR amplification step with different combinations of oligonucleotide pairs (0.6 μM each) in a total volume of 50 μl, with a 0.2 mM concentration (each) of the four deoxynucleoside triphosphates and 1.5 U of Pfu DNA polymerase (Promega) and in the commercial buffer.

    Techniques: Agarose Gel Electrophoresis, Generated

    CNA as terminator of polymerization. A) Set of primers used for each PCR. Forward primer was 32 P labelled to detect only the sense strand. The lengths of the PCR products expected are indicated. B) Denaturing polyacrylamide gel electrophoresis obtained for each set of primers with Taq DNA polymerase (1–5) and Pfu DNA polymerase (6–10). For each polymerase, the PCR were performed with different reverse primers: R W (lanes 1, 10); R CNA (lanes 2, 9); R 2CNA (lanes 3, 8); R 2Peg (lanes 4, 7); R (lanes 5, 6). The length of the mains fragments is indicated. Inset: gel overexposed.

    Journal: PLoS ONE

    Article Title: ?,?-D-Constrained Nucleic Acids Are Strong Terminators of Thermostable DNA Polymerases in Polymerase Chain Reaction

    doi: 10.1371/journal.pone.0025510

    Figure Lengend Snippet: CNA as terminator of polymerization. A) Set of primers used for each PCR. Forward primer was 32 P labelled to detect only the sense strand. The lengths of the PCR products expected are indicated. B) Denaturing polyacrylamide gel electrophoresis obtained for each set of primers with Taq DNA polymerase (1–5) and Pfu DNA polymerase (6–10). For each polymerase, the PCR were performed with different reverse primers: R W (lanes 1, 10); R CNA (lanes 2, 9); R 2CNA (lanes 3, 8); R 2Peg (lanes 4, 7); R (lanes 5, 6). The length of the mains fragments is indicated. Inset: gel overexposed.

    Article Snippet: Pfu DNA polymerase was purchased from Promega (Madison, USA).

    Techniques: Polymerase Chain Reaction, Polyacrylamide Gel Electrophoresis