pfu dna polymerases  (Thermo Fisher)


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    Thermo Fisher pfu dna polymerases
    Pfu Dna Polymerases, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfu dna polymerases/product/Thermo Fisher
    Average 89 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    pfu dna polymerases - by Bioz Stars, 2020-05
    89/100 stars

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    Article Title: Rational design of Pleurotus eryngii versatile ligninolytic peroxidase for enhanced pH and thermal stability through structure-based protein engineering.
    Article Snippet: Pfu DNA polymerases from Fermentas (Pittsburgh, Pennsylvania, USA).

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    Thermo Fisher pfu dna polymerase
    Results of ‘RNA-induced <t>DNA</t> replication interference’ assays. a Effects of 27nt-RNAs using <t>Pfu</t> or Taq polymerases after end-point-PCR and agarose gel electrophoresis (top) or after qPCR (bottom). b Effects of 27nt-RNAs alone or in combination with PIWI1 on linear DNA amplification in a Klenow reaction were assayed via qPCR. c Hypothetical models on sequence-specific targeting through Argonaute/PIWI-RNA complexes (blue: IES, red: MDS, yellow: PIWI1, green: 27nt-RNA, orange: tethered transcript [ c1 only]): c1 According to the ‚nascent transcript model, 27nt-RNA/PIWI1 complexes could target tethered IES-originating transcripts, which would be reminiscent of observations made in divergent eukaryotes, such as S. pombe , C. elegans and A. thaliana ]). Alternatively, we assumed that 27nt-RNA/PIWI1 complexes could interact with dsDNA ( c2 ) or via base-pairing with ssDNA, possibly when it occurs in a replication bubble ( c3 ). d Mapping of mRNA reads purified 20 h PC on micronuclear model genes reveals that IES (red bars) are sharply omitted
    Pfu Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 542 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfu dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 542 article reviews
    Price from $9.99 to $1999.99
    pfu dna polymerase - by Bioz Stars, 2020-05
    99/100 stars
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    Results of ‘RNA-induced DNA replication interference’ assays. a Effects of 27nt-RNAs using Pfu or Taq polymerases after end-point-PCR and agarose gel electrophoresis (top) or after qPCR (bottom). b Effects of 27nt-RNAs alone or in combination with PIWI1 on linear DNA amplification in a Klenow reaction were assayed via qPCR. c Hypothetical models on sequence-specific targeting through Argonaute/PIWI-RNA complexes (blue: IES, red: MDS, yellow: PIWI1, green: 27nt-RNA, orange: tethered transcript [ c1 only]): c1 According to the ‚nascent transcript model, 27nt-RNA/PIWI1 complexes could target tethered IES-originating transcripts, which would be reminiscent of observations made in divergent eukaryotes, such as S. pombe , C. elegans and A. thaliana ]). Alternatively, we assumed that 27nt-RNA/PIWI1 complexes could interact with dsDNA ( c2 ) or via base-pairing with ssDNA, possibly when it occurs in a replication bubble ( c3 ). d Mapping of mRNA reads purified 20 h PC on micronuclear model genes reveals that IES (red bars) are sharply omitted

    Journal: Epigenetics & Chromatin

    Article Title: 27nt-RNAs guide histone variant deposition via ‘RNA-induced DNA replication interference’ and thus transmit parental genome partitioning in Stylonychia

    doi: 10.1186/s13072-018-0201-5

    Figure Lengend Snippet: Results of ‘RNA-induced DNA replication interference’ assays. a Effects of 27nt-RNAs using Pfu or Taq polymerases after end-point-PCR and agarose gel electrophoresis (top) or after qPCR (bottom). b Effects of 27nt-RNAs alone or in combination with PIWI1 on linear DNA amplification in a Klenow reaction were assayed via qPCR. c Hypothetical models on sequence-specific targeting through Argonaute/PIWI-RNA complexes (blue: IES, red: MDS, yellow: PIWI1, green: 27nt-RNA, orange: tethered transcript [ c1 only]): c1 According to the ‚nascent transcript model, 27nt-RNA/PIWI1 complexes could target tethered IES-originating transcripts, which would be reminiscent of observations made in divergent eukaryotes, such as S. pombe , C. elegans and A. thaliana ]). Alternatively, we assumed that 27nt-RNA/PIWI1 complexes could interact with dsDNA ( c2 ) or via base-pairing with ssDNA, possibly when it occurs in a replication bubble ( c3 ). d Mapping of mRNA reads purified 20 h PC on micronuclear model genes reveals that IES (red bars) are sharply omitted

    Article Snippet: Standard PCR conditions were as follows: 95 °C for 10 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 30 s], 72 °C for 10 min; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 45 s], 72 °C for 10 min. Quantitative PCR was performed on a Rotor Gene 6000 (Corbett Life Science) using QuantiTect SYBR green Taq polymerase Master Mix (Qiagen) or Pfu DNA polymerase (ThermoFisher) and SYBR green (Sigma-Aldrich).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Real-time Polymerase Chain Reaction, Amplification, Sequencing, Purification

    Cloning and sub-cloning of CD19 cDNA. A) Amplification of specific band for human CD19 cDNA using Pfu DNA polymerase; B) Colony-PCR reaction on eight white colonies (1-8) after blue/ white selection. C) Excision of 1701 bp band for human CD19 cDNA after double digestion of the construct using KpnI and HindIII restriction enzymes. Lanes (a) and (a’): undigested pGEMT-easy/CD19 construct, Lanes (b) partial and complete digestion by KpnI and Hind III, respectively and (b’): complete digestion by both KpnI and HindIII. SM: DNA size marker ( bp ). Asterisks (*) point the desired band.

    Journal: Avicenna Journal of Medical Biotechnology

    Article Title: Cloning and Expression of CD19, a Human B-Cell Marker in NIH-3T3 Cell Line

    doi:

    Figure Lengend Snippet: Cloning and sub-cloning of CD19 cDNA. A) Amplification of specific band for human CD19 cDNA using Pfu DNA polymerase; B) Colony-PCR reaction on eight white colonies (1-8) after blue/ white selection. C) Excision of 1701 bp band for human CD19 cDNA after double digestion of the construct using KpnI and HindIII restriction enzymes. Lanes (a) and (a’): undigested pGEMT-easy/CD19 construct, Lanes (b) partial and complete digestion by KpnI and Hind III, respectively and (b’): complete digestion by both KpnI and HindIII. SM: DNA size marker ( bp ). Asterisks (*) point the desired band.

    Article Snippet: For amplification of CD19 cDNA, PCR was performed in a 25 µl reaction solution contained 2.5 µl 10×PCR buffer, 1.5 µl 10 mM dNTPs, 1 µl of each primer (10 pmol/µl ), 0.5 µl Pfu DNA polymerase (10 U/µl ) (Thermo Fisher Scientific, Inc., MA, USA), 1 mM MgSO4 and 1 µl cDNA.

    Techniques: Clone Assay, Subcloning, Amplification, Polymerase Chain Reaction, Selection, Construct, Marker

    Results of ‘RNA-induced DNA replication interference’ assays. a Effects of 27nt-RNAs using Pfu or Taq polymerases after end-point-PCR and agarose gel electropho resis (top) or after qPCR (bottom). b Effects of 27nt-RNAs alone or in combination with PIWI1 on linear DNA amplification in a Klenow reaction were assayed via qPCR. c Hypothetical models on sequence-specific targeting through Argonaute/PIWI-RNA complexes (blue: IES, red: MDS, yellow: PIWI1, green: 27nt-RNA, orange: tethered transcript [ c1 only]): c1 According to the ‚nascent transcript model, 27nt-RNA/PIWI1 complexes could target tethered IES-originating transcripts, which would be reminiscent of observations made in divergent eukaryotes, such as S. pombe , C. elegans and A. thaliana (reviewed in [ 38 ]). Alternatively, we assumed that 27nt-RNA/PIWI1 complexes could interact with dsDNA ( c2 ) or via base-pairing with ssDNA, possibly when it occurs in a replication bubble ( c3 ). d Mapping of mRNA reads purified 20 h PC on micronuclear model genes reveals that IES (red bars) are sharply omitted

    Journal: Epigenetics & Chromatin

    Article Title: 27nt-RNAs guide histone variant deposition via ‘RNA-induced DNA replication interference’ and thus transmit parental genome partitioning in Stylonychia

    doi: 10.1186/s13072-018-0201-5

    Figure Lengend Snippet: Results of ‘RNA-induced DNA replication interference’ assays. a Effects of 27nt-RNAs using Pfu or Taq polymerases after end-point-PCR and agarose gel electropho resis (top) or after qPCR (bottom). b Effects of 27nt-RNAs alone or in combination with PIWI1 on linear DNA amplification in a Klenow reaction were assayed via qPCR. c Hypothetical models on sequence-specific targeting through Argonaute/PIWI-RNA complexes (blue: IES, red: MDS, yellow: PIWI1, green: 27nt-RNA, orange: tethered transcript [ c1 only]): c1 According to the ‚nascent transcript model, 27nt-RNA/PIWI1 complexes could target tethered IES-originating transcripts, which would be reminiscent of observations made in divergent eukaryotes, such as S. pombe , C. elegans and A. thaliana (reviewed in [ 38 ]). Alternatively, we assumed that 27nt-RNA/PIWI1 complexes could interact with dsDNA ( c2 ) or via base-pairing with ssDNA, possibly when it occurs in a replication bubble ( c3 ). d Mapping of mRNA reads purified 20 h PC on micronuclear model genes reveals that IES (red bars) are sharply omitted

    Article Snippet: Standard PCR conditions were as follows: 95 °C for 10 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 30 s], 72 °C for 10 min; alternatively, for the 856 bp PCNA amplicon the conditions were: 95 °C for 15 min, 30 cycles of [95 °C for 15 s, 60 °C for 30 s, 72 °C for 45 s], 72 °C for 10 min. Quantitative PCR was performed on a Rotor Gene 6000 (Corbett Life Science) using QuantiTect SYBR green Taq polymerase Master Mix (Qiagen) or Pfu DNA polymerase (ThermoFisher) and SYBR green (Sigma-Aldrich).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Real-time Polymerase Chain Reaction, Amplification, Sequencing, Purification